• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Csp

General Information
Symbol	Dmel\Csp	Species	D. melanogaster
Name	Cysteine string protein	Annotation symbol	CG6395
Feature type	protein_coding_gene	FlyBase ID	FBgn0004179
Gene Model Status	Current	Stock availability	15 publicly available
Also Known As	Dcsp, l(3)03988, ab49, csp32
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	79D3-79D3	Sequence location	3L:22,260,668..22,266,845 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Cysteine string protein is referred to in FlyBase by the symbol Dmel\Csp (CG6395, FBgn0004179). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: startle response; brain morphogenesis; exocytosis; locomotion involved in locomotory behavior. 58 alleles are reported. The phenotypes of these alleles are annotated with: eye; interommatidial bristle; neuromuscular junction; embryonic/larval neuromuscular junction; wing; ommatidium. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: DnaJ domain; DnaJ domain, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, adult spermathecae, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, adult heart, larval/adult fat body, larval trachea, adult ovary, adult testis, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1636651_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Csp. Gene sequence location is 3L:22260668..22266845.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 79D3-79D3   Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
Experimentally Determined Cytological Location
Cytogenetic map Notes References 79E1-79E2     (Zinsmaier, 1998.4.1) 79E1-79E2   (determined by in situ hybridisation)   (Spradling et al., 1999) 79E1-79E2   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999) 79E-79E   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Csp for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Csp-RA FBtr0078493  NM_079489   2832   223 Csp-RB FBtr0078494  NM_168949   1842   249 Csp-RC FBtr0078495  NM_168950   2226   228 Csp-RD FBtr0334746  NM_001275277   1498   244 Csp-RE FBtr0334747  NM_001275278   2895   244
Additional Transcript Data & Comments
Reported size (kB)	2.9, 2.3 (northern blot) (Zinsmaier et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Csp-PA   FBpp0078145   23.8   223   5.03     NP_524213   AAF51817 Csp-PB   FBpp0078146   26.9   249   5.32     NP_730713   AAF51816 Csp-PC   FBpp0078147   24.6   228   5.04     NP_730714   AAN12195 Csp-PD   FBpp0306787   26.2   244   5.30     NP_001262206   AGB94899 Csp-PE   FBpp0306788   26.2   244   5.30     NP_001262207   AGB94900
Additional Polypeptide Data & Comments
Reported size (kDa)	249, 223 (aa); 34, 32 (kD observed); 27, 24 (kD predicted) (Zinsmaier et al., 1990)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DnaJ domain, conserved site (IPR018253) DnaJ domain (IPR001623)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202659   AA247010   AA263633   AA439907   AA440606   AA440832   AA538849   AA567160   AA567533   AA694674   AA696269   AA697940   AA817468   AA817608   AA940649   AA951337   AE014296 AAF51816   AE014296 AAF51817   AE014296 AAN12195   AF057167 AAD09428   AF057167 AAD09430   AF057167 AAD09431   AE014296 AGB94899   AE014296 AGB94900   AI107970   AI258660   AI295100   AI456738   AI512951   AI516919   AI531175   AQ026193   AQ073777   AQ254596   AW944216   AX664476   AY059457 AAL13363   BF494041   BG632963   BG638811   BI163046   BI163751   BI164415   BI164773   BI164815   BI166704   BI167734   BI169104   BI170009   BI170883   BI173285   BI173286   BI213455   BI216709   BI228877   BI229679   BI229698   BI229712   BI232685   BI234529   BI234974   BI238824   BI240253   BI240789   BI243453   BI368457   BI370447   BI373326   BI373886   BI375661   BI376044   BI587720   BI635153   BP556773   BT088851   BT100236   CK659009   CO177532   CO178341   CO178785   CO179279   CO179452   CO187213   CO187492   CO188244   CO188528   CO190790   CO192211   CO196435   CO196588   CO262297   CO262665   CO262823   CO262844   CO263404   CO264011   CO264748   CO267252   CO272341   CO273926   CO274518   CO287349   CO287471   CO287962   CO289488   CO289503   CO289628   CO290071   CO290722   CO293589   CO293613   CO295704   CO296985   CO303296   CO304575   CO304800   CO307128   CO310256   CO313415   CO314433   CO314913   CO316775   CO316837   CO316871   CO320655   CO322264   CO324814   CO329464   CO329830   CO331022   CZ467398   CZ467599   CZ475564   CZ484195   EC056850   EC056853   EC060810   EC060990   EC068609   EC069084   EC069290   EC072087   EC081139   EC081643   EC081790   EC081977   EC082763   EC088413   EC195904   EC196043   EC196158   EC196632   EC196695   EC198079   EC200880   EC201292   EC203212   EC204033   EC206419   EC207332   EC208078   EC208232   EC210506   EC213184   EC214199   EC215935   EC218366   EC219103   EC219128   EC219489   EC224657   EC226493   EC227802   EC227996   EC228623   EC229040   EC229449   EC230065   EC231125   EC233010   EC235998   EC236572   EC237631   EC238521   EC239257   EC239382   EC240820   EC242560   EC242697   EC244218   EC246444   EC249862   EC250707   EC256261   EV581340   EV586379   EV588349   M63008 AAA28431   M63421 AAA28432
UniProtKB/Swiss-Prot	Q03751
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage   adult head   (Zinsmaier et al., 1990) radioisotope in situ Stage Tissue/Position (including subcellular localization) Reference adult stage   adult brain   (Zinsmaier et al., 1990) retina   (Zinsmaier et al., 1990)
Additional Descriptive Data
	Csp transcripts are detected in the retina and in the entire brain cellular rind. (Zinsmaier et al., 1990) Csp transcripts are detected in adult head RNA on northern blots. (Zinsmaier et al., 1990)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference larval stage   neuromuscular junction   (Zinsmaier et al., 1994) third instar larval stage   embryonic/larval neuromuscular junction   (Zelhof et al., 2001) Comment:presynaptic marker embryonic/larval neuromuscular junction   • terminal bouton (Bronk et al., 2001) embryonic/larval central nervous system   (Mohtashami et al., 2001) adult stage   lamina neuropil   (Zinsmaier et al., 1994) photoreceptor cell   (Zinsmaier et al., 1994) neuron   • synaptic vesicle (Zinsmaier et al., 1994) bouton   (Zinsmaier et al., 1994) lobula plate   (Zinsmaier et al., 1994) neuromuscular junction of adult thorax   (Zinsmaier et al., 1994) photoreceptor cell   • cytoplasm of terminal bouton (Hiesinger et al., 2001) lamina neuropil | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R1 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R2 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R3 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R4 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R5 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R6 | restricted   (Hamanaka and Meinertzhagen, 2010) medulla   (Zinsmaier et al., 1994) medulla | restricted   (Hamanaka and Meinertzhagen, 2010)
Additional Descriptive Data
	Csp protein is enriched in the neuropil of the larval ventral ganglion, where synapses occur. (Mohtashami et al., 2001) Csp is expressed in central brain neuropils. It is broadly seen in the R1-R6 terminals, but appears especially to line the borders facing the cartridge interior. Using immunoelectron microscopy, Csp is detected in the photoreceptor cells close to the T-bar ribbon surrounded by synaptic vesicles. (Hamanaka and Meinertzhagen, 2010) Csp protein is detected presynaptically at larval and adult neuromuscular junctions. Synaptic boutons are strongly stained. In the CNS, Synapse rich regions of the neuropil are stained, but the surrounding cell bodies are not. One isoform is detected predominantly in photoreceptor terminals but not at motor nerve terminals. Csp protein is thought to be associated with synaptic vesicles. (Zinsmaier et al., 1994)
Marker for
	synaptic vesicle
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010) synaptic vesicle inferred from direct assay (Jiao et al., 2010) terminal bouton inferred from direct assay (Loveall and Deitcher, 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, adult spermathecae, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, adult heart, larval/adult fat body, larval trachea, adult ovary, adult testis, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1134.15 Larval Midgut    926 Larval Hindgut    736.4 Larval Malpighian Tubules    553.7 Larval Fat Body    239.6 Larval Salivary Gland    810.5 Larval Trachea    493.775 Larval Carcass    476.95 Adult Head    588.2 Adult Eye    905.35 Adult Brain    1093.9 Adult Thoracic-Abdominal Ganglion    1385.6 Adult Crop    444.8 Adult Midgut    571.4 Adult Hindgut    456.8 Adult Malpighian Tubules    911.5 Adult Fat Body    305.4 Adult Salivary Gland    707 Adult Heart    214.85 Adult VirginFemale Spermatheca    698.9 Adult InseminatedFemale Spermatheca    993.1 Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland    772 Adult Carcass    189 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1134.15) Larval Midgut  (926) Larval Hindgut  (736.4) Larval Malpighian Tubules  (553.7) Larval Fat Body    239.6 Larval Salivary Gland  (810.5) Larval Trachea    493.775 Larval Carcass    476.95 Adult Head  (588.2) Adult Eye  (905.35) Adult Brain  (1093.9) Adult Thoracic-Abdominal Ganglion  (1385.6) Adult Crop    444.8 Adult Midgut  (571.4) Adult Hindgut    456.8 Adult Malpighian Tubules  (911.5) Adult Fat Body    305.4 Adult Salivary Gland  (707) Adult Heart    214.85 Adult VirginFemale Spermatheca  (698.9) Adult InseminatedFemale Spermatheca  (993.1) Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland  (772) Adult Carcass    189 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1134.15) Larval Midgut    926 Larval Hindgut    736.4 Larval Malpighian Tubules    553.7 Larval Fat Body    239.6 Larval Salivary Gland    810.5 Larval Trachea    493.775 Larval Carcass    476.95 Adult Head    588.2 Adult Eye    905.35 Adult Brain  (1093.9) Adult Thoracic-Abdominal Ganglion  (1385.6) Adult Crop    444.8 Adult Midgut    571.4 Adult Hindgut    456.8 Adult Malpighian Tubules    911.5 Adult Fat Body    305.4 Adult Salivary Gland    707 Adult Heart    214.85 Adult VirginFemale Spermatheca    698.9 Adult InseminatedFemale Spermatheca    993.1 Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland    772 Adult Carcass    189 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1134.15 Larval Midgut    926 Larval Hindgut    736.4 Larval Malpighian Tubules    553.7 Larval Fat Body    239.6 Larval Salivary Gland    810.5 Larval Trachea    493.775 Larval Carcass    476.95 Adult Head    588.2 Adult Eye    905.35 Adult Brain    1093.9 Adult Thoracic-Abdominal Ganglion    1385.6 Adult Crop    444.8 Adult Midgut    571.4 Adult Hindgut    456.8 Adult Malpighian Tubules    911.5 Adult Fat Body    305.4 Adult Salivary Gland    707 Adult Heart    214.85 Adult VirginFemale Spermatheca    698.9 Adult InseminatedFemale Spermatheca    993.1 Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland    772 Adult Carcass    189 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1134.15 Larval Midgut    926 Larval Hindgut    736.4 Larval Malpighian Tubules    553.7 Larval Fat Body    239.6 Larval Salivary Gland    810.5 Larval Trachea    493.775 Larval Carcass    476.95 Adult Head    588.2 Adult Eye    905.35 Adult Brain    1093.9 Adult Thoracic-Abdominal Ganglion    1385.6 Adult Crop    444.8 Adult Midgut    571.4 Adult Hindgut    456.8 Adult Malpighian Tubules    911.5 Adult Fat Body    305.4 Adult Salivary Gland    707 Adult Heart    214.85 Adult VirginFemale Spermatheca    698.9 Adult InseminatedFemale Spermatheca    993.1 Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland    772 Adult Carcass    189 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1134.15) Larval Midgut  (926) Larval Hindgut  736.4 Larval Malpighian Tubules  553.7 Larval Fat Body    239.6 Larval Salivary Gland  810.5 Larval Trachea    493.775 Larval Carcass    476.95 Adult Head  588.2 Adult Eye  (905.35) Adult Brain  (1093.9) Adult Thoracic-Abdominal Ganglion  (1385.6) Adult Crop    444.8 Adult Midgut  571.4 Adult Hindgut    456.8 Adult Malpighian Tubules  (911.5) Adult Fat Body    305.4 Adult Salivary Gland  707 Adult Heart    214.85 Adult VirginFemale Spermatheca  698.9 Adult InseminatedFemale Spermatheca  (993.1) Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland  772 Adult Carcass    189 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1134.15 Larval Midgut    926 Larval Hindgut    736.4 Larval Malpighian Tubules    553.7 Larval Fat Body    239.6 Larval Salivary Gland    810.5 Larval Trachea    493.775 Larval Carcass    476.95 Adult Head    588.2 Adult Eye    905.35 Adult Brain  1093.9 Adult Thoracic-Abdominal Ganglion  1385.6 Adult Crop    444.8 Adult Midgut    571.4 Adult Hindgut    456.8 Adult Malpighian Tubules    911.5 Adult Fat Body    305.4 Adult Salivary Gland    707 Adult Heart    214.85 Adult VirginFemale Spermatheca    698.9 Adult InseminatedFemale Spermatheca    993.1 Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland    772 Adult Carcass    189 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1134.15 Larval Midgut    926 Larval Hindgut    736.4 Larval Malpighian Tubules    553.7 Larval Fat Body    239.6 Larval Salivary Gland    810.5 Larval Trachea    493.775 Larval Carcass    476.95 Adult Head    588.2 Adult Eye    905.35 Adult Brain    1093.9 Adult Thoracic-Abdominal Ganglion    1385.6 Adult Crop    444.8 Adult Midgut    571.4 Adult Hindgut    456.8 Adult Malpighian Tubules    911.5 Adult Fat Body    305.4 Adult Salivary Gland    707 Adult Heart    214.85 Adult VirginFemale Spermatheca    698.9 Adult InseminatedFemale Spermatheca    993.1 Adult Ovary    417.2 Adult Testis    102.4 Adult Male Accessory Gland    772 Adult Carcass    189 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    588.2   NA  Eye    905.35   NA  Brain    1093.9   1134.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1385.6   NA  Crop    444.8   926  Midgut    571.4   736.4  Hindgut    456.8   553.7  Malpighian Tubules    911.5   239.6  Fat Body    305.4   810.5  Salivary Gland    707   NA  Heart    214.85   493.775  Trachea    NA   NA  VirginFemale Spermatheca    698.9   NA  InseminatedFemale Spermatheca    993.1   NA  Ovary    417.2   NA  Testis    102.4   NA  Male Accessory Gland    772   476.95  Carcass    189 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    68 central nervous system, pupae P8    94 head, virgin 1-day female    75 head, virgin 4-day female    51 head, virgin 20-day female    35 head, mated 1-day female    60 head, mated 4-day female    48 head, mated 20-day female    33 head, mated 1-day male    108 head, mated 4-day male    93 head, mated 20-day male    80 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    48 digestive system, larvae L3 wandering    53 digestive system, 1-day adult    44 digestive system, 4-day adult    43 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    29 fat body, pupae P8    36 carcass, larvae L3 wandering    43 carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female    92 ovary, mated 4-day female    72 testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (49) central nervous system, larvae L3  (68) central nervous system, pupae P8  (94) head, virgin 1-day female  (75) head, virgin 4-day female  (51) head, virgin 20-day female  (35) head, mated 1-day female  (60) head, mated 4-day female  (48) head, mated 20-day female  (33) head, mated 1-day male  (108) head, mated 4-day male  (93) head, mated 20-day male  (80) salivary gland, larvae L3 wandering    18 salivary gland, white prepupae  (48) digestive system, larvae L3 wandering  (53) digestive system, 1-day adult  (44) digestive system, 4-day adult  (43) digestive system, 20-day adult  (39) fat body, larvae L3 wandering    7 fat body, white prepupae  (29) fat body, pupae P8  (36) carcass, larvae L3 wandering  (43) carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female  (92) ovary, mated 4-day female  (72) testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    68 central nervous system, pupae P8    94 head, virgin 1-day female    75 head, virgin 4-day female    51 head, virgin 20-day female    35 head, mated 1-day female    60 head, mated 4-day female    48 head, mated 20-day female    33 head, mated 1-day male  (108) head, mated 4-day male    93 head, mated 20-day male    80 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    48 digestive system, larvae L3 wandering    53 digestive system, 1-day adult    44 digestive system, 4-day adult    43 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    29 fat body, pupae P8    36 carcass, larvae L3 wandering    43 carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female    92 ovary, mated 4-day female    72 testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    68 central nervous system, pupae P8    94 head, virgin 1-day female    75 head, virgin 4-day female    51 head, virgin 20-day female    35 head, mated 1-day female    60 head, mated 4-day female    48 head, mated 20-day female    33 head, mated 1-day male    108 head, mated 4-day male    93 head, mated 20-day male    80 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    48 digestive system, larvae L3 wandering    53 digestive system, 1-day adult    44 digestive system, 4-day adult    43 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    29 fat body, pupae P8    36 carcass, larvae L3 wandering    43 carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female    92 ovary, mated 4-day female    72 testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    68 central nervous system, pupae P8    94 head, virgin 1-day female    75 head, virgin 4-day female    51 head, virgin 20-day female    35 head, mated 1-day female    60 head, mated 4-day female    48 head, mated 20-day female    33 head, mated 1-day male    108 head, mated 4-day male    93 head, mated 20-day male    80 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    48 digestive system, larvae L3 wandering    53 digestive system, 1-day adult    44 digestive system, 4-day adult    43 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    29 fat body, pupae P8    36 carcass, larvae L3 wandering    43 carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female    92 ovary, mated 4-day female    72 testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (49) central nervous system, larvae L3  (68) central nervous system, pupae P8  (94) head, virgin 1-day female  (75) head, virgin 4-day female  (51) head, virgin 20-day female  (35) head, mated 1-day female  (60) head, mated 4-day female  (48) head, mated 20-day female  (33) head, mated 1-day male  (108) head, mated 4-day male  (93) head, mated 20-day male  (80) salivary gland, larvae L3 wandering    18 salivary gland, white prepupae  (48) digestive system, larvae L3 wandering  (53) digestive system, 1-day adult  (44) digestive system, 4-day adult  (43) digestive system, 20-day adult  (39) fat body, larvae L3 wandering    7 fat body, white prepupae  29 fat body, pupae P8  (36) carcass, larvae L3 wandering  (43) carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female  (92) ovary, mated 4-day female  (72) testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    68 central nervous system, pupae P8    94 head, virgin 1-day female    75 head, virgin 4-day female    51 head, virgin 20-day female    35 head, mated 1-day female    60 head, mated 4-day female    48 head, mated 20-day female    33 head, mated 1-day male  108 head, mated 4-day male    93 head, mated 20-day male    80 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    48 digestive system, larvae L3 wandering    53 digestive system, 1-day adult    44 digestive system, 4-day adult    43 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    29 fat body, pupae P8    36 carcass, larvae L3 wandering    43 carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female    92 ovary, mated 4-day female    72 testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    68 central nervous system, pupae P8    94 head, virgin 1-day female    75 head, virgin 4-day female    51 head, virgin 20-day female    35 head, mated 1-day female    60 head, mated 4-day female    48 head, mated 20-day female    33 head, mated 1-day male    108 head, mated 4-day male    93 head, mated 20-day male    80 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    48 digestive system, larvae L3 wandering    53 digestive system, 1-day adult    44 digestive system, 4-day adult    43 digestive system, 20-day adult    39 fat body, larvae L3 wandering    7 fat body, white prepupae    29 fat body, pupae P8    36 carcass, larvae L3 wandering    43 carcass, 1-day adult    20 carcass, 4-day adult    22 carcass, 20-day adult    18 ovary, virgin 4-day female    92 ovary, mated 4-day female    72 testis, mated 4-day male    24 accessory gland, mated 4-day male    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    90 embryo 04-06hr    71 embryo 06-08hr    68 embryo 08-10hr    58 embryo 10-12hr    29 embryo 12-14hr    42 embryo 14-16hr    31 embryo 16-18hr    48 embryo 18-20hr    40 embryo 20-22hr    56 embryo 22-24hr    64 larva L1    38 larva L2    33 larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    42 white prepupae new    43 white prepupae 12hr    41 white prepupae 24hr    44 pupae 2d postWPP    43 pupae 3d postWPP    36 pupae 4d postWPP    29 adult male 01day    33 adult male 05day    33 adult male 30day    26 adult female 01day    32 adult female 05day    48 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (64) embryo 02-04hr  (90) embryo 04-06hr  (71) embryo 06-08hr  (68) embryo 08-10hr  (58) embryo 10-12hr  (29) embryo 12-14hr  (42) embryo 14-16hr  (31) embryo 16-18hr  (48) embryo 18-20hr  (40) embryo 20-22hr  (56) embryo 22-24hr  (64) larva L1  (38) larva L2  (33) larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  (30) larva L3 puffstage 7-9  (42) white prepupae new  (43) white prepupae 12hr  (41) white prepupae 24hr  (44) pupae 2d postWPP  (43) pupae 3d postWPP  (36) pupae 4d postWPP  (29) adult male 01day  (33) adult male 05day  (33) adult male 30day  26 adult female 01day  (32) adult female 05day  (48) adult female 30day  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    90 embryo 04-06hr    71 embryo 06-08hr    68 embryo 08-10hr    58 embryo 10-12hr    29 embryo 12-14hr    42 embryo 14-16hr    31 embryo 16-18hr    48 embryo 18-20hr    40 embryo 20-22hr    56 embryo 22-24hr    64 larva L1    38 larva L2    33 larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    42 white prepupae new    43 white prepupae 12hr    41 white prepupae 24hr    44 pupae 2d postWPP    43 pupae 3d postWPP    36 pupae 4d postWPP    29 adult male 01day    33 adult male 05day    33 adult male 30day    26 adult female 01day    32 adult female 05day    48 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    90 embryo 04-06hr    71 embryo 06-08hr    68 embryo 08-10hr    58 embryo 10-12hr    29 embryo 12-14hr    42 embryo 14-16hr    31 embryo 16-18hr    48 embryo 18-20hr    40 embryo 20-22hr    56 embryo 22-24hr    64 larva L1    38 larva L2    33 larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    42 white prepupae new    43 white prepupae 12hr    41 white prepupae 24hr    44 pupae 2d postWPP    43 pupae 3d postWPP    36 pupae 4d postWPP    29 adult male 01day    33 adult male 05day    33 adult male 30day    26 adult female 01day    32 adult female 05day    48 adult female 30day    47 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    90 embryo 04-06hr    71 embryo 06-08hr    68 embryo 08-10hr    58 embryo 10-12hr    29 embryo 12-14hr    42 embryo 14-16hr    31 embryo 16-18hr    48 embryo 18-20hr    40 embryo 20-22hr    56 embryo 22-24hr    64 larva L1    38 larva L2    33 larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    42 white prepupae new    43 white prepupae 12hr    41 white prepupae 24hr    44 pupae 2d postWPP    43 pupae 3d postWPP    36 pupae 4d postWPP    29 adult male 01day    33 adult male 05day    33 adult male 30day    26 adult female 01day    32 adult female 05day    48 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (64) embryo 02-04hr  (90) embryo 04-06hr  (71) embryo 06-08hr  (68) embryo 08-10hr  (58) embryo 10-12hr  29 embryo 12-14hr  (42) embryo 14-16hr  31 embryo 16-18hr  (48) embryo 18-20hr  (40) embryo 20-22hr  (56) embryo 22-24hr  (64) larva L1  (38) larva L2  (33) larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  30 larva L3 puffstage 7-9  (42) white prepupae new  (43) white prepupae 12hr  (41) white prepupae 24hr  (44) pupae 2d postWPP  (43) pupae 3d postWPP  (36) pupae 4d postWPP  29 adult male 01day  (33) adult male 05day  (33) adult male 30day  26 adult female 01day  32 adult female 05day  (48) adult female 30day  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    90 embryo 04-06hr    71 embryo 06-08hr    68 embryo 08-10hr    58 embryo 10-12hr    29 embryo 12-14hr    42 embryo 14-16hr    31 embryo 16-18hr    48 embryo 18-20hr    40 embryo 20-22hr    56 embryo 22-24hr    64 larva L1    38 larva L2    33 larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    42 white prepupae new    43 white prepupae 12hr    41 white prepupae 24hr    44 pupae 2d postWPP    43 pupae 3d postWPP    36 pupae 4d postWPP    29 adult male 01day    33 adult male 05day    33 adult male 30day    26 adult female 01day    32 adult female 05day    48 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    90 embryo 04-06hr    71 embryo 06-08hr    68 embryo 08-10hr    58 embryo 10-12hr    29 embryo 12-14hr    42 embryo 14-16hr    31 embryo 16-18hr    48 embryo 18-20hr    40 embryo 20-22hr    56 embryo 22-24hr    64 larva L1    38 larva L2    33 larva L3 12hr old    22 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    42 white prepupae new    43 white prepupae 12hr    41 white prepupae 24hr    44 pupae 2d postWPP    43 pupae 3d postWPP    36 pupae 4d postWPP    29 adult male 01day    33 adult male 05day    33 adult male 30day    26 adult female 01day    32 adult female 05day    48 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    52 embryonic 1182-4H    53 embryonic GM2    165 embryonic Kc167    39 embryonic S1    43 embryonic S3    81 leg disc CME L1    35 wing disc CME-W2    79 wing disc ML-DmD8    21 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    21 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    41 mixed discs ML-DmD4-c1    42 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    89 ovary fGS/OSS    36 ovary OSC    39 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (70) Schneider line 2 Sg4  (52) embryonic 1182-4H  (53) embryonic GM2  (165) embryonic Kc167  (39) embryonic S1  (43) embryonic S3  (81) leg disc CME L1  (35) wing disc CME-W2  (79) wing disc ML-DmD8    21 wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  (53) wing disc ML-DmD21    21 wing disc ML-DmD32  (44) haltere disc ML-DmD17-c3  (61) eye-antennal disc ML-DmD11  (34) antennal disc ML-DmD20-c5  (41) mixed discs ML-DmD4-c1  (42) CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  (62) tumorous blood cells mbn2  (89) ovary fGS/OSS  (36) ovary OSC  (39) ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    52 embryonic 1182-4H    53 embryonic GM2  (165) embryonic Kc167    39 embryonic S1    43 embryonic S3    81 leg disc CME L1    35 wing disc CME-W2    79 wing disc ML-DmD8    21 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    21 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    41 mixed discs ML-DmD4-c1    42 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    89 ovary fGS/OSS    36 ovary OSC    39 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    52 embryonic 1182-4H    53 embryonic GM2    165 embryonic Kc167    39 embryonic S1    43 embryonic S3    81 leg disc CME L1    35 wing disc CME-W2    79 wing disc ML-DmD8    21 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    21 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    41 mixed discs ML-DmD4-c1    42 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    89 ovary fGS/OSS    36 ovary OSC    39 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    52 embryonic 1182-4H    53 embryonic GM2    165 embryonic Kc167    39 embryonic S1    43 embryonic S3    81 leg disc CME L1    35 wing disc CME-W2    79 wing disc ML-DmD8    21 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    21 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    41 mixed discs ML-DmD4-c1    42 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    89 ovary fGS/OSS    36 ovary OSC    39 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (70) Schneider line 2 Sg4  (52) embryonic 1182-4H  (53) embryonic GM2  (165) embryonic Kc167  (39) embryonic S1  (43) embryonic S3  (81) leg disc CME L1  (35) wing disc CME-W2  (79) wing disc ML-DmD8    21 wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  (53) wing disc ML-DmD21    21 wing disc ML-DmD32  (44) haltere disc ML-DmD17-c3  (61) eye-antennal disc ML-DmD11  (34) antennal disc ML-DmD20-c5  (41) mixed discs ML-DmD4-c1  (42) CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  (62) tumorous blood cells mbn2  (89) ovary fGS/OSS  (36) ovary OSC  (39) ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    52 embryonic 1182-4H    53 embryonic GM2  (165) embryonic Kc167    39 embryonic S1    43 embryonic S3    81 leg disc CME L1    35 wing disc CME-W2    79 wing disc ML-DmD8    21 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    21 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    41 mixed discs ML-DmD4-c1    42 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    89 ovary fGS/OSS    36 ovary OSC    39 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    52 embryonic 1182-4H    53 embryonic GM2    165 embryonic Kc167    39 embryonic S1    43 embryonic S3    81 leg disc CME L1    35 wing disc CME-W2    79 wing disc ML-DmD8    21 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    21 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    41 mixed discs ML-DmD4-c1    42 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    89 ovary fGS/OSS    36 ovary OSC    39 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    46 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    66 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    43 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult  (34) Cadmium 50 mM 6 hrs, larvae L3  (31) Cadmium 50 mM 12 hrs, larvae L3  (46) Cadmium 50 mM 48 hrs, 4-day adult  (41) Cadmium 100 mM 48 hrs, 4-day adult  (66) Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult  (61) Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult  (50) Ethanol 2.5% 3 hrs, larvae L3  (48) Ethanol 5% 3 hrs, larvae L3  (42) Ethanol 10% 3 hrs, larvae L3  (27) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (28) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (60) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (56) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3  (43) Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    46 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    66 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    43 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    46 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    66 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    43 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    46 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    66 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    43 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult  (34) Cadmium 50 mM 6 hrs, larvae L3  31 Cadmium 50 mM 12 hrs, larvae L3  (46) Cadmium 50 mM 48 hrs, 4-day adult  (41) Cadmium 100 mM 48 hrs, 4-day adult  (66) Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult  (61) Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult  (50) Ethanol 2.5% 3 hrs, larvae L3  (48) Ethanol 5% 3 hrs, larvae L3  (42) Ethanol 10% 3 hrs, larvae L3  27 Caffeine 1.5 mg/ml 4 hrs, larvae L3  28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (60) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (56) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3  (43) Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    46 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    66 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    43 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    16 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    46 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    66 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    43 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004179 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6395-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal CspCB02217 lethal | embryonic stage | heat sensitive, with Scer\GAL4elav-C155 Csp1.Scer\UAS lethal | embryonic stage | heat sensitive, with Scer\GAL4hs.PB Csp1.Scer\UAS lethal | larval stage | heat sensitive, with Scer\GAL4elav-C155 Csp2.Scer\UAS Csp3.Scer\UAS lethal | larval stage | heat sensitive, with Scer\GAL4hs.PB Csp2.Scer\UAS Csp3.Scer\UAS lethal | partially | recessive | heat sensitive CspU1 lethal | recessive Csp03988 partially lethal - majority die Csp03988 partially lethal - majority die | recessive Csp03988 CspR1 CspU1 CspX1 viable CspK16 CspU1 CspU2 CspU3 CspU4 viable, with Scer\GAL4pnr-MD237 CspGD10571 CspGD16551 viable | reduced | heat sensitive CspX1 CspX2 Other Phenotypes Allele behavior defective, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS courtship behavior defective | male CspX1 hypoactive, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS neurophysiology defective CspR1 CspU1 CspX1 neurophysiology defective | heat sensitive CspR1 CspX1 neurophysiology defective | larval stage | recessive CspR1 neurophysiology defective | recessive CspE16 neurophysiology defective | third instar larval stage (with CspU1) CspX1 neurophysiology defective | third instar larval stage (with CspX1) CspU1 optomotor response defective | recessive CspU1w paralytic | adult stage | heat sensitive CspU1 paralytic | conditional | recessive CspU1 paralytic | heat sensitive CspE1 CspE2 CspR1 CspU1 CspX1 paralytic | recessive | heat sensitive CspU1 CspU1w CspU2 CspU3 CspU4 CspX1 CspX2 short lived, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS short lived (with CspU1) CspU1w short lived (with CspU1w) CspU1 short lived | recessive CspU1 uncoordinated | adult stage, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS uncoordinated | recessive CspU1 visible, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS wild-type Csp+t12.3 Phenotype manifest in Allele axon | larval stage CspU1 CspX1 embryonic/larval neuromuscular junction (with CspU1) CspX1 embryonic/larval neuromuscular junction (with CspX1) CspU1 eye, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS germline cell | male, with Scer\GAL4ptc-559.1 CspEP659 interommatidial bristle, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS neuromuscular junction CspU1 CspX1 neuromuscular junction | larval stage CspR1 ommatidium, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS wing, with Scer\GAL4elav-C155 Csp1.Scer\UAS Csp2.Scer\UAS Csp3.Scer\UAS
Classical Alleles ( 37 )
For All Classical Alleles Show
Allele of Csp Class Mutagen Stocks Known lesion Cspc01730 piggyBac transposase 1 -- Cspc01985 piggyBac transposase 1 -- Cspd03979 1 -- Cspd07162 P-element activity 1 Yes CspEY22488 P-element activity 1 -- Cspf01725 piggyBac transposase 1 -- CspNP2813 1 -- CspX1 heat sensitive loss of function allele gamma ray 1 Yes Csp03988 P-element activity 0 -- CspCB02217 P-element activity 0 Yes CspDG29203 P-element activity 0 -- CspE16 loss of function allele 0 -- CspE1 ethyl methanesulfonate 0 -- CspE2 ethyl methanesulfonate 0 -- CspE46 loss of function allele 0 Yes CspE95 hypomorphic allele - genetic evidence 0 Yes CspEP3141 P-element activity 0 -- CspEP659 P-element activity 0 -- CspGS9167 0 Yes CspK16 0 Yes CspP1 P-element activity 0 Yes CspR1 loss of function allele, heat sensitive loss of function allele 0 Yes CspR2 Delta2-3 0 Yes CspS3 loss of function allele 0 Yes CspU1 unequal recombination 0 Yes CspU1w P-element activity 0 Yes CspU2 heat-treatment 0 Yes CspU3 heat-treatment 0 -- CspU4 heat-treatment 0 -- Cspunspecified 0 -- CspX18 0 Yes CspX2 gamma ray 0 Yes CspX4 gamma ray 0 -- CspX5 gamma ray 0 -- CspX6 gamma ray 0 -- CspX7 gamma ray 0 -- Cspμ1 0 --
Alleles Carried on Transgenic Constructs ( 21 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Csp Class Mutagen Stocks Known lesion CspGD10571 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CspGD16551 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CspHMS00046 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CspJF01235 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CspJF01462 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CspKK109431 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Csp+t12.3 in vitro construct - genomic fragment 0 Yes Csp1.Scer\UAS in vitro construct - regulatory fusion 0 Yes Csp2.Scer\UAS in vitro construct - regulatory fusion 0 Yes Csp3.Scer\UAS in vitro construct - regulatory fusion 0 Yes CspClp in vitro construct - amino acid replacement in vitro construct - deletion 0 Yes CspCslp in vitro construct - deletion 0 Yes CspCΔ27 in vitro construct - deletion 0 Yes CsplcDNA1 in vitro construct - regulatory fusion 0 Yes CspLΔ8 in vitro construct - deletion 0 Yes CspNIG.6395R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes CspscDNA1 in vitro construct - regulatory fusion 0 Yes CspScsp in vitro construct - deletion 0 Yes CspSsp.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes CspSsp in vitro construct - amino acid replacement 0 Yes CsptCH322-06D09 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Csp-X3 (Eberle et al., 1998) In(3)CspR2 (Eberle et al., 1998)
Not disrupted in	Df(3L)Pc-MK (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10571} NA P{GD16551} NA P{KK109431} NA P{NIG.6395R} NA P{TRiP.HMS00046} NA P{TRiP.JF01235} NA P{TRiP.JF01462} NA P{UAS-Csp.1} NA P{UAS-Csp.2} NA P{UAS-Csp.3} NA P{UAS-Csp.Ssp} NA characterization construct P{Csp12.3} NA P{CspClp} NA P{CspCslp} NA P{CspCΔ27} NA P{CsplcDNA1} NA P{CspLΔ8} NA P{CspscDNA1} NA P{CspScsp} NA P{CspSsp} NA P{UAS-n-sybTMD-csp2} NA PBac{CH322-06D09} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}CspP1 No P{lacW}srpk79DP1 No P{PZ}Csp03988 No insertion of mobile activating element P{EP}CspEP659 NA P{EP}CspEP3141 NA P{EPgy2}CspEY22488 NA P{GSV6}CspGS9167 NA P{XP}Cspd03979 NA P{XP}Cspd07162 NA miscellaneous insertions P{3'lacW}CspX1 NA P{?lacW}CspU1 NA P{PTT-GB}CspCB02217 NA P{wHy}Aats-ileDG09701 NA P{wHy}CspDG29203 NA PBac{PB}Cspc01730 NA PBac{PB}Cspc01985 NA PBac{WH}Cspf01725 NA roo{}CspU2 NA insertion of enhancer trap binary system P{GawB}CspNP2813 No
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
brain morphogenesis inferred from mutant phenotype (Yamamoto et al., 2008) exocytosis inferred from mutant phenotype (Dawson-Scully et al., 2000) locomotion involved in locomotory behavior inferred from mutant phenotype (Yamamoto et al., 2008) startle response inferred from mutant phenotype (Yamamoto et al., 2008)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010) synaptic vesicle inferred from direct assay (Jiao et al., 2010) terminal bouton inferred from direct assay (Loveall and Deitcher, 2010)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
neurotransmitter secretion traceable author statement (Stimson and Ramaswami, 1999) protein folding non-traceable author statement (Littleton et al., 1999) synaptic vesicle exocytosis inferred from sequence or structural similarity (Littleton, 2000) synaptic vesicle uncoating traceable author statement (Stimson and Ramaswami, 1999) vesicle-mediated transport traceable author statement (Stimson and Ramaswami, 1999)
Cellular Component
CV term Evidence References
plasma membrane inferred from sequence or structural similarity (Zinsmaier et al., 1990) synaptic vesicle traceable author statement (Stimson and Ramaswami, 1999)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Csp - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Arc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Arp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Arpc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Arpc4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Arpc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG10887 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG11876 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG12262 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG12567 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG1458 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG14722 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG15098 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG2070 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG2691 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG31694 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG31739 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG4278 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG4291 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG6543 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG6724 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG7033 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG7834 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG8801 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG9253 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - CG9281 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Cyp12d1-p anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Dp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Droj2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Ect4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Gnf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - HIP-R anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hlc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hsp22 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hsp27 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hsp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hsp70Aa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Hsp70Ba anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Ip259 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Nurf-38 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - P32 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - PNUTS anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Prosα2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Prosα6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Prosα7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Prosβ3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Prosβ5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Prp31 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - R anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Rbm13 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - RhoGAP18B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - RpS10a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - RpS10b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - RpS29 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Rpt1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Rs1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Srp19 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Srp72 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Surf6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Tapδ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - Trap1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - U2af38 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - ball anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - cg anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - eIF6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - mRpL2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - me31B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - mod(mdg4) anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - par-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - ref(2)P anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - sgl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - sqh anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Csp - tws anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Csp allele Gene References Cspunspecified Hsc70-4 (Wenniger et al., 1998) CspX1 Hsc70-4 (Bronk et al., 2001) unspecified pbl (Gregory et al., 2007) Syx1A (Nie et al., 1999)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65696 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004179 DroID - A comprehensive database of gene and protein interactions. • FBgn0004179 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40459
Orthologs
OrthoDB Orthologs (51) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6RNBVP Dipteran inclusive ortholog search EOG68PMV8 Insect inclusive ortholog search EOG6M385Q Arthropod inclusive ortholog search EOG69GHZP Metazoa inclusive ortholog search EOG6J74KX
Orthologs in Drosophila Species (EOG6RNBVP)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Csp     Drosophila simulans   Dsim\GD17653 Y   Drosophila sechellia   Dsec\GM22437 Y   Drosophila erecta   Dere\GG16248 Y   Drosophila yakuba   Dyak\GE22607 Y   Drosophila ananassae   Dana\GF10972 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19562 Y   Drosophila persimilis   Dper\GL20793 Y   Drosophila willistoni   Dwil\GK17108 Y   Drosophila virilis   Dvir\GJ11627 Y   Drosophila mojavensis   Dmoj\GI11373 Y   Drosophila grimshawi   Dgri\GH16687 Y
Orthologs in non-Drosophila Dipterans (EOG68PMV8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001569   Anopheles gambiae Malaria mosquito  Agam\AGAP007620   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017369
Orthologs in non-Dipteran Insects (EOG6M385Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49934   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001423   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH24405   Atta cephalotes Leafcutter ant  Acep\ACEP21685   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22517   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18786   Linepithema humile Argentine ant  Lhum\LH19636   Pogonomyrmex barbatus Red harvester ant  Pbar\PB10655   Solenopsis invicta Red fire ant  Sinv\SINV11496   Acyrthosiphon pisum Pea aphid  Apim\ACYPI072174   Bombyx mori Silkmoth  Bmor\BGIBMGA007808   Pediculus humanus Human body louse  Phum\PHUM247130   Tribolium castaneum Red flour beetle  Tcas\GLEAN_15301
Orthologs in non-Insect Arthropods (EOG69GHZP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_110882   Daphnia pulex Water flea  Dpux\JGI_V11_308045   Ixodes scapularis Deer tick  Isca\ISCW015127
Orthologs in non-Arthropod Metazoa (EOG6J74KX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\dnj-14   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016611gn   Danio rerio Zebrafish  Drer\dnajc5aa   Danio rerio Zebrafish  Drer\dnajc5b   Danio rerio Zebrafish  Drer\dnajc5gb   Danio rerio Zebrafish  Drer\dnajc5ab   Danio rerio Zebrafish  Drer\dnajc5g   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000013034   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000030170   Gallus gallus Domestic chicken  Ggal\DNAJC5B   Gallus gallus Domestic chicken  Ggal\DNAJC5   Mus musculus House mouse  Mmus\Dnajc5   Mus musculus House mouse  Mmus\Dnajc5b   Mus musculus House mouse  Mmus\Dnajc5g   Rattus norvegicus Norway rat  Rnor\Dnajc5b   Rattus norvegicus Norway rat  Rnor\Dnajc5g   Rattus norvegicus Norway rat  Rnor\Dnajc5   Homo sapiens Human  Hsap\DNAJC5   Homo sapiens Human  Hsap\FLJ40417   Homo sapiens Human  Hsap\DNAJC5B
Human Orthologs (3)
Gene OMIM HGNC Hsap\DNAJC5     Hsap\FLJ40417     Hsap\DNAJC5B
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD17653 Drosophila sechellia Dsec\GM22437 Drosophila erecta Dere\GG16248 Drosophila yakuba Dyak\GE22607 Drosophila ananassae Dana\GF10972 Drosophila pseudoobscura pseudoobscura Dpse\GA19562 Drosophila persimilis Dper\GL20793 Drosophila willistoni Dwil\GK17108 Drosophila virilis Dvir\GJ11627 Drosophila mojavensis Dmoj\GI11373 Drosophila grimshawi Dgri\GH16687
Stocks & Reagents
Stocks Listed in FlyBase ( 15 )
Bloomington	32035 CspX1/TM3, Sb1 31290 y1 v1; P{TRiP.JF01235}attP2 31669 y1 v1; P{TRiP.JF01462}attP2
Harvard	- P{XP}Cspd03979 - P{XP}Cspd07162 - PBac{PB}Cspc01730 - PBac{PB}Cspc01985 - PBac{WH}Cspf01725
VDRC	v103201 P{KK109431}VIE-260B v47208 w1118; P{GD16551}v47208 v34167 w1118; P{GD10571}v34167/TM3 v34168 w1118; P{GD10571}v34168/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR48E10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 175 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD06525 SD02108
Other clones	DPiM_LD06525 IP16506
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02448 GH05881 GH17293 GH27523 GM01170 GM01223 GM05405 HL01409 HL03501 LD02834 LD06113 LD06963 LD14593 LD15439 LD15738 LD18471 LD22172 LD22944 LD23591 LD31750 LD45111 LP01907 LP08632 LP16408 RE02343 RE03243 RE04082 RE04517 RE04563 RE06906 RE08185 RE10082 RE11343 RE12486 RE16075 RE16076 RE18860 RE23932 RE26534 RE27512 RE27524 RE27548 RE28789 RE30240 RE30757 RE36954 RE37714 RE41103 RE41893 RE54429 RE56967 RE60586 RE61317 RE63524 RE63965 RH28574 SD16406
Other clones	11685 13004 28768 29296 31276 31389 37402 62159 66782 74082 77695 79014 82240 98462 117421 119167 120956 123069 149656 161684 161733 161831 176517 182714 190962 191131 191354 194414 214567.45 CK01.1A.G3 CK01.1B.G7 CK01.10A.D4 CK01.19C.B4 CK01.27A.F2 CK01.29A.F3 CK01.31C.E7 CK01.33D.E5 CK01.51C.D7 CK01.61D.E2 CK01.66A.C8 CK01.71A.D6 CK01.72C.C12 CK01.75B.A10 CK01.76A.H7 CK01.85A.G3 CK01.90D.D8 CK01.91C.G1 CK01.91D.E1 CK01.96C.D2 CK01.98C.G11 CK01.99D.E1 CK01.102A.B6 CK01.102D.B8 CK01.102D.E11 CK01.105D.A8 CK01.114A.D3 Dmel_dig_010835_0722_0732 Dmel_neb_007601_0121_3618 Dmel_neb_012265_0185_0514 EC33360 EK004414 EK006328 EK027313 EK030015 EK038723 EK040816 EK049649 EK053744 EK056447 EK057542 EK058935 EK059432 EK065624 EK065935 EK067426 EK071148 EK071209 EK071625 EK073004 EK074945 EK085004 EK086039 EK086440 EK086518 EK088436 EK106807 EK112646 EK114329 EK137151 EK140242 EK149240 EK187752 EK194911 EK203803 EK203840 EK211360 EK215615 EK220918 EK232903 EK236224 EK238355 EK240920 EK245201 EK246246 EK253702 EK265833 EK266022 EK266066 EK286831 EK292915 EK293957 EK297264 FGM305D12 LD36856
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004179 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40459
Antibody Information
	monoclonal (Zinsmaier et al., 1994)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Csp CG6395 (Beasley, 1999.11) Source for identity of: Csp CG6395 (Worley, 1999.11)
Source for database merge of	Source for merge of: Csp anon-WO02059370.50 (FlyBase, 1996-) Source for merge of: Csp l(3)03988 (Spradling et al., 1999)
Additional comments	Source for merge of Csp anon-WO02059370.50 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Csp has multiple functions at synaptic terminals. Csp plays a direct role in exocytosis downstream from Ca2+ entry. Csp seems to increase the Ca2+ sensitivity of the exocytotic machinery. (Dawson-Scully et al., 2000) Mutations in Csp affect calcium secretion coupling of evoked exocytosis but not vesicle recycling. (Ranjan et al., 1998) Fluorescent Ca-sensitive dye, Ca Crimson, is used to monitor presynaptic Ca dynamics. (Umbach et al., 1998) An analysis of the excitatory postsynaptic currents from depolarized motor nerve terminals leads to the conclusion that Csp protein is involved in neurotransmitter release and helps to synchronise evoked neurotransmitter release at nerve terminals. (Heckmann et al., 1997) Biochemical characterisation of Csp suggests that fatty acyl groups do not form the main anchor of Csp in membranes as the complete removal of this modification does not dissociate Csp from the membrane. (van de Goor and Kelly, 1996) n-syb and Csp proteins are targetted to synaptic vesicles. (van de Goor et al., 1995) Neuromuscular transmission is impaired presynaptically in Csp mutant larvae. Csp mutations disrupt depolarisation-secretion coupling, depolarisation fails to trigger transmitter release. This disruption explains the cellular basis of the temperature sensitive paralysis of the mutant larvae. (Umbach et al., 1994) A RNA helicase, member of the DEAD box protein family, is the adjacent transcript unit to Csp that expresses two RNA transcripts. (Zinsmaier et al., 1994) Csp gene product is required for stabilizing the components of the neurotransmitter release machinery, lack of Csp gene product causes block of synaptic transmissions at high temperatures. (Zinsmaier et al., 1994) Csp expression pattern has been studied: MAB ab49 antibodies were used to screen cDNA expression libraries to identify clones coding for recognized protein fragments. (Zinsmaier et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079489 NM_168949 NM_168950 NM_001275277 NM_001275278 RefSeq (Proteins) • NP_524213 NP_730713 NP_730714 NP_001262206 NP_001262207 DDBJ / EMBL / GenBank • AA567160 AAA28431 AAA28432 AAD09428 AAD09430 AAD09431 AAF51816 AAF51817 AAL13363 AAN12195 AF057167 AGB94899 AGB94900 AI531175 AQ026193 AQ073777 AQ254596 AW944216 AX664476 AY059457 CZ467398 CZ467599 CZ475564 CZ484195 M63008 M63421 Entrez Gene - A searchable database of RefSeq genes. • 40459 UniProtKB/Swiss-Prot • Q03751
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DnaJ domain, conserved site (IPR018253) DnaJ domain (IPR001623)
Linkouts
	BioGRID - A database of protein and genetic interactions • 65696 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004179 DroID - A comprehensive database of gene and protein interactions. • FBgn0004179 DRSC - Results from RNAi screens. • FBgn0004179 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004179 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6395-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004179 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40459 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40459 modMine - Data generated by the modENCODE project. • FBgn0004179
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	ab49 (Botella et al., 2003, Song et al., 2002) anon-WO02059370.50   CG6395 (Verstreken and Bellen, 2000.9.4, Whitfield, 2000.4.28, Keleman et al., 2009.8.5, Khanna et al., 2010, Ni et al., 2008, Ni et al., 2010.12.1) CSP (Fabian-Fine et al., 2003, Wucherpfennig et al., 2003, Koh et al., 2004, Verstreken et al., 2003, Guichet et al., 2002, Mathew et al., 2002, Verstreken et al., 2002, Gunawardena and Goldstein, 2001, Bronk et al., 2001, Lloyd et al., 2000, Zinsmaier, 1998.4.1, Beumer et al., 1999, Reist et al., 1998, Gonzalez-Gaitan and Jackle, 1997, Linial and Parnas, 1996, Bate and Broadie, 1995, Dudu et al., 2006, Ataman, 2006, Wagh et al., 2006, Jin et al., 2012, Rivlin et al., 2004, Liebl et al., 2008, Miśkiewicz et al., 2011, Bao et al., 2007, Chouhan et al., 2010, Chan et al., 2011, Mosca and Schwarz, 2010, Barkus et al., 2008, Wu et al., 2005, Stowers and Isacoff, 2007, Huang et al., 2006, Martin et al., 2005, Murthy et al., 2005, Chen et al., 2002, Aravamudan and Broadie, 2003, Wang et al., 2011, Zeng et al., 2007, Lorenzo et al., 2010, Kumar et al., 2009, Brooks et al., 2011, Chu et al., 2006, Besse et al., 2007, Consoulas et al., 2005, Rezával et al., 2008, Baqri et al., 2006, Rister and Heisenberg, 2006, Toda et al., 2008, Fischer et al., 2009, Edwards and Meinertzhagen, 2009, Gilestro et al., 2009, Ren et al., 2009, Mast et al., 2008, Caygill and Johnston, 2008, Rolls et al., 2007, Zhang et al., 2009, Wagner et al., 2007, Johnson et al., 2009, Bao et al., 2005, Hamanaka et al., 2010, Ratnaparkhi et al., 2008, Ayyub et al., 2005, Borycz et al., 2008, Loveall and Deitcher, 2010, Jiao et al., 2010, Zhang et al., 2007, Hamanaka and Meinertzhagen, 2010, Paddock et al., 2011, Lee et al., 2010, Liu et al., 2011, Uytterhoeven et al., 2011, Moua et al., 2011, Matos et al., 2000, Fei et al., 2010, Williamson et al., 2010, Henthorn et al., 2011, Dimitroff et al., 2012, Füger et al., 2012, Kim et al., 2012, Beck et al., 2012, Vijayakrishnan et al., 2010) csp (Wellington and Zinsmaier, 2005, McCabe et al., 2003, Estes et al., 2003, Botella et al., 2003, Barclay et al., 2002, Hebbar and Fernandes, 2002, Bronk et al., 2001, Saitoe et al., 2001, Saitoe et al., 2001, Verstreken and Bellen, 2001, Bellen, 2000.12.25, Hiesinger et al., 2001, Nie et al., 1999, Wu et al., 1999, Rohrbough et al., 1999, Stimson et al., 1998, Eberle et al., 1998, Peixoto and Hall, 1998, Wenniger et al., 1998, Wu and Bellen, 1997, Ranjan et al., 1998, Umbach and Gundersen, 1997, Heckmann et al., 1997, Parfitt et al., 1995, Estes et al., 1996, Littleton et al., 1995, Zinsmaier et al., 1993, Eberle et al., 1993, Umbach et al., 1994, Zinsmaier et al., 1994, Zinsmaier and Benzer, 1991, Ohyama et al., 2007, Bronk et al., 2005, Serpe and O'Connor, 2006, Bugga et al., 2009, Mudiganti et al., 2010, Besse et al., 2007, Dickman and Davis, 2009, Estes et al., 2000, Scantlebury et al., 2007, Mohtashami et al., 2001, Sarthi and Elefant, 2011) Csp (Gregory et al., 2007, Takaesu et al., 2006, Koh et al., 2007, Buszczak et al., 2007, Arnold et al., 2004, Wong et al., 2005, Goold and Davis, 2007, Yamamoto et al., 2008, Kerman et al., 2008, Ayroles et al., 2009, Dierick and Greenspan, 2006, Lin et al., 2009, Mozer and Sandstrom, 2012, Blard et al., 2007, Chen et al., 2008, Venken et al., 2009, Fuentes-Medel et al., 2009, Johnson et al., 2009, Cherry and Perrimon, 2004, Nieratschker et al., 2009, Gleason, 2005, Rossetto et al., 2011, Li et al., 2010, Cook et al., 2012, Bridon et al., 2012, Yamamoto et al., 2009, Japanese National Institute of Genetics, 2012.5.21) csp29 (Zinsmaier, 1993.1.19) csp32 (Zinsmaier, 1993.1.19) CSP32 (Iliopoulos et al., 1997) Cys (Gleason and Ritchie, 2004) D.M.CSP (Becker et al., 2001) Dcsp (Pahud et al., 1998, van de Goor and Kelly, 1996, van de Goor et al., 1995, Ganetzky, 1994) D-CSP (Buchner and Gundersen, 1997) DCSP-1 (Reichmuth et al., 1995) DSP/DNAJC5 (Wishart et al., 2012) l(3)03988 (Berkeley Drosophila Genome Project, 1993.6.1, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, ) MAB ab49 (Reichmuth et al., 1995)
Name Synonym	cysteine string protein (Verstreken and Bellen, 2002, Acharya et al., 2006, Barkus et al., 2008, Arnold et al., 2004, Stowers and Isacoff, 2007, Huang et al., 2006, Martin et al., 2005, Haghnia et al., 2007, Chen et al., 2002, Lorenzo et al., 2010, Hebbar et al., 2006, Toda et al., 2008, Edwards and Meinertzhagen, 2009, Ren et al., 2009, Caygill and Johnston, 2008, Rolls et al., 2007, Zhang et al., 2009, Cherry and Perrimon, 2004, Estes et al., 2000, Hamanaka et al., 2010, Nieratschker et al., 2009, Borycz et al., 2008, Loveall and Deitcher, 2010, de Haro et al., 2010, Scantlebury et al., 2007, Henthorn et al., 2011, Nahm et al., 2010, Bridon et al., 2012, Kim et al., 2012, Beck et al., 2012) Cysteine string protein (Koh et al., 2007, Yamamoto et al., 2008, Shalaby et al., 2009, Blard et al., 2007, Fuentes-Medel et al., 2009, Jordan et al., 2007, Fei et al., 2010, Gleason, 2005, Sarthi and Elefant, 2011, Yamamoto et al., 2009) Cysteine String Protein (Andrews et al., 2002, Mosca and Schwarz, 2010, Aravamudan and Broadie, 2003, Chu et al., 2006, Mozer and Sandstrom, 2012, Johnson et al., 2009, Ayyub et al., 2005, Dimitroff et al., 2012) cysteine-string protein (Bao et al., 2007, Chouhan et al., 2010, Wu et al., 2005, Bao et al., 2005, Liu et al., 2011, Lanson et al., 2011, Vijayakrishnan et al., 2010) Cysteine string protein 3 (Khanna et al., 2010) cystein string protein (Kurshan et al., 2009, Rezával et al., 2008, Baqri et al., 2006, Gilestro et al., 2009, Cook et al., 2012)
Secondary FlyBase IDs
	FBgn0010827 FBgn0066009
References ( 273 )
Generate a list of	All references relating to this gene ( 273 )
List References by type	Research paper  ( 205 ) Supplementary material  ( 7 ) Review  ( 14 ) Personal communication to FlyBase  ( 17 ) Abstract  ( 17 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 4 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Patent  ( 1 ) Letter  ( 1 )
Recent research papers ( 22 )
	Beck et al., 2012, J. Neurosci. 32(20): 7058--7073 Regulation of Fasciclin II and Synaptic Terminal Development by the Splicing Factor Beag. [FBrf0218385] Bridon et al., 2012, J. Proteome Res. 11(2): 927--940 Improvement of Phosphoproteome Analyses Using FAIMS and Decision Tree Fragmentation. Application to the Insulin Signaling Pathway in Drosophila melanogaster S2 Cells. [FBrf0217365] Cook et al., 2012, PLoS ONE 7(9): e44440 Increased RhoA Prenylation in the loechrig (loe) Mutant Leads to Progressive Neurodegeneration. [FBrf0219441] Dimitroff et al., 2012, PLoS ONE 7(2): e30722 Diet and Energy-Sensing Inputs Affect TorC1-Mediated Axon Misrouting but Not TorC2-Directed Synapse Growth in a Drosophila Model of Tuberous Sclerosis. [FBrf0217461] Füger et al., 2012, PLoS Genet. 8(11): e1003066 Spastic Paraplegia Mutation N256S in the Neuronal Microtubule Motor KIF5A Disrupts Axonal Transport in a Drosophila HSP Model. [FBrf0220146] Jin et al., 2012, PLoS ONE 7(7): e40912 Similarities of Drosophila rab GTPases Based on Expression Profiling: Completion and Analysis of the rab-Gal4 Kit. [FBrf0219021] Kim et al., 2012, Genes Dev. 26(9): 974--987 Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction. [FBrf0218161] Lagisz et al., 2012, Heredity 108(6): 602--608 Two distinct genomic regions, harbouring the period and fruitless genes, affect male courtship song in Drosophila montana. [FBrf0218390] Mozer and Sandstrom, 2012, Mol. Cell. Neurosci. 51(3-4): 89--100 Drosophila neuroligin 1 regulates synaptic growth and function in response to activity and phosphoinositide-3-kinase. [FBrf0219839] Wishart et al., 2012, PLoS Genet. 8(8): e1002936 Combining comparative proteomics and molecular genetics uncovers regulators of synaptic and axonal stability and degeneration in vivo. [FBrf0219374] Brooks et al., 2011, Neuron 72(2): 316--329 A putative vesicular transporter expressed in Drosophila mushroom bodies that mediates sexual behavior may define a neurotransmitter system. [FBrf0216456] Chan et al., 2011, Curr. Biol. 21(20): 1704--1715 Systematic discovery of rab GTPases with synaptic functions in Drosophila. [FBrf0216517] Henthorn et al., 2011, Mol. Biol. Cell 22(21): 4038--4046 A role for kinesin heavy chain in controlling vesicle transport into dendrites in Drosophila. [FBrf0216511] Lanson et al., 2011, Hum. Mol. Genet. 20(13): 2510--2523 A Drosophila model of FUS-related neurodegeneration reveals genetic interaction between FUS and TDP-43. [FBrf0213887] Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Miśkiewicz et al., 2011, Neuron 72(5): 776--788 ELP3 Controls Active Zone Morphology by Acetylating the ELKS Family Member Bruchpilot. [FBrf0216903] Moua et al., 2011, Development 138(6): 1087--1092 Kinesin-1 tail autoregulation and microtubule-binding regions function in saltatory transport but not ooplasmic streaming. [FBrf0213072] Paddock et al., 2011, J. Neurosci. 31(6): 2248--2257 Membrane penetration by synaptotagmin is required for coupling calcium binding to vesicle fusion in vivo. [FBrf0212995] Rossetto et al., 2011, Hum. Mol. Genet. 20(21): 4248--4257 Defhc1.1, a homologue of the juvenile myoclonic gene EFHC1, modulates architecture and basal activity of the neuromuscular junction in Drosophila. [FBrf0216330] Sarthi and Elefant, 2011, PLoS ONE 6(10): e26202 dTip60 HAT Activity Controls Synaptic Bouton Expansion at the Drosophila Neuromuscular Junction. [FBrf0216592] Uytterhoeven et al., 2011, Cell 145(1): 117--132 Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins. [FBrf0213384] Wang et al., 2011, J. Clin. Invest. 121(10): 4118--4126 The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span. [FBrf0216240] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...