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Gene Dmel\nonA

General Information
Symbol	Dmel\nonA	Species	D. melanogaster
Name	no on or off transient A	Annotation symbol	CG4211
Feature type	protein_coding_gene	FlyBase ID	FBgn0004227
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	diss, non-A, Bj6
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	14B18-14C1	Sequence location	X:16,255,602..16,265,580 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene no on or off transient A is referred to in FlyBase by the symbol Dmel\nonA (CG4211, FBgn0004227). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: poly-pyrimidine tract binding; mRNA binding. There is experimental evidence that it is involved in the biological process: male courtship behavior, veined wing generated song production; visual perception. 42 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: NOPS; Nucleotide-binding, alpha-beta plait; RNA recognition motif domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Gene sequence location is X:16255602..16265580.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	nonA: no on or off transient-A (J.C. Hall) Defective optomotor responses and phototaxis, with the former being especially defective (Heisenberg, 1972; Heisenberg and Buchner, 1977; Kulkarni et al., 1988). Poor orientation behavior in Y-maze (Bulthoff, 1982a, b). nonA5 flies exhibit specific lack of responses to front-to-back moving visual stimuli, whereas reaction to back-to-front motion is intact (Heisenberg, 1972). Physiologically, there are reduced or absent light-on and light-off transient spikes in electroretinogram, whereas photoreceptor potential is normal (Hotta and Benzer, 1970; Pak et al., 1970; Heisenberg, 1971; Kulkarni et al., 1988; Jones and Rubin, 1990). Larval visual response (re negative phototaxis) normal [Hotta and Keng, 1984, Animal Behavior: Neurophysiological and Ethological Approaches (Aoki et al., eds.). Springer-Verlag, Berlin, pp. 49-60]. Courtship song is abnormal, as influenced by one, possibly two, mutant alleles; nonA9 (originally diss) males produce abnormal song, regarding pulses relatively late in trains of such song sounds (each one resulting from a bout of wing vibration); the abnormalities are polycyclicity (Kulkarni et al., 1988) and anomalous intra-pulse frequency components (Wheeler et al., 1989); pulses early in trains, or throughout short ones, are nearly normal; courtship hum sounds manifest irregular sine waves, though their fundamental frequencies are normal (Wheeler et al., 1989). nonA9 by itself is also an optomotor-defective/ERG-abnormal mutant (Kulkarni et al., 1988) and fails to complement other mutant alleles with regard to these visual phenotypes (Rendahl, Kulkarni, and Hall, unpublished); but these heterozygotes, in a homozygous tra genetic background, sing normally (with the possible exception of nonA2/nonA9, Rendahl et al., unpublished), as do males hemizygous for alleles isolated as visual mutants.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 14B18-14C1   Limits computationally determined from genome sequence between P{EP}Cyp1EP1073&P{EP}Dsp1EP355 and P{EP}EP1493EP1493
Experimentally Determined Cytological Location
Cytogenetic map Notes References 14B-14C   (determined by in situ hybridisation)   (Jones and Rubin, 1990) 14C1-14C2   (determined by in situ hybridisation)   (von Besser et al., 1990)
Experimentally Determined Recombination Data
Location	1-56 (Heisenberg, 1971) 1-52 (Kulkarni et al., 1988) 1-52.3 (Pak et al., 1970)
Left of (cM)	f (Kulkarni et al., 1988)
Right of (cM)	v g sd (Kulkarni et al., 1988)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nonA for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nonA-RA FBtr0074232  NM_078643   3747   700 nonA-RB FBtr0074233  NM_167505   2358   742 nonA-RC FBtr0100586  NM_001014747   5008   698 nonA-RD FBtr0340313     6746   700
Additional Transcript Data & Comments
Reported size (kB)	4.0, 3.0, 2.8 (northern blot) (Jones and Rubin, 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nonA-PA   FBpp0074011   77.0   700   9.97     NP_523367   AAF48597 nonA-PB   FBpp0074012   82.0   742   9.67     NP_727962   AAN09398 nonA-PC   FBpp0100043   77.0   698   9.80     NP_001014747   AAX52501 nonA-PD   FBpp0309274   77.0   700   9.97
Additional Polypeptide Data & Comments
Reported size (kDa)	82 (kD observed) (Frasch and Saumweber, 1989) 82 (kD) (Buchenau et al., 1993) 80 (kD) (Rendahl et al., 1992)
Comments	form II (FlyBase, 1992-) form I (FlyBase, 1992-) The RNA-binding motif of nonA protein is most similar to those of Sxl protein, the polyA-binding proteins, and tra2 protein. (Rendahl et al., 1992) The two nonA proteins differ only in the C-terminal 33 (or 35) amino acids. Transformation experiments show that the 700aa protein is required for the function of nonA in vision. (Jones and Rubin, 1990, Jones and Rubin, 1990)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677) NOPS (IPR012975)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202891   AA390377   AA390674   AA392433   AA440059   AA440710   AA541091   AA735332   AA735358   AA803774   AA951461   AE014298 AAF48597   AE014298 AAN09398   AE014298 AAX52501   AI107825   AI261127   AI403744   AI516954   AI946267   AJ296020   AY119651 AAM50305   BI165074   BI170675   BI171224   BI171887   BI231436   BI237162   BI241210   BI241781   BI242498   BI243530   BI352240   BI355039   BI370475   BI371402   BI485481   BI563879   BI574489   BI586657   BI617816   BI621111   BI624956   BI626400   BI632244   BP558476   BT149890   CK135245   CK659799   CK660496   CO186040   CO188661   CO190746   CO196295   CO265768   CO267002   CO271671   CO271745   CO275800   CO275981   CO276690   CO277169   CO278288   CO287779   CO287835   CO292897   CO293834   CO294796   CO295486   CO296747   CO297677   CO304218   CO307499   CO309181   CO312984   CO314782   CO316768   CO317088   CO317962   CO319179   CO322973   CO323362   CO324300   CO328209   CO330872   CO331511   CO331930   CO335863   CO340025   CO340484   CO340667   CO341157   CO345056   CZ490682   DN154191   EC058604   EC058870   EC060182   EC060233   EC063316   EC066120   EC066521   EC067517   EC074980   EC075361   EC079652   EC079981   EC084552   EC086606   EC086969   EC089203   EC090221   EC194514   EC196125   EC200175   EC200649   EC200656   EC205218   EC208924   EC213884   EC213893   EC216400   EC216478   EC217576   EC220464   EC224807   EC227427   EC233140   EC236033   EC236344   EC237421   EC240702   EC241149   EC247248   EC247345   EC252677   EC253310   EC254708   EC255441   EC257049   EC260544   EC263182   EL886004   GH945749   GH946663   GH948146   GH954586   GH958169   GH958627   GH972024   GH972394   GH979378   GH981046   M33496 AAA03214   M33496 AAA03215   X55902 CAA39395   Z31966   Z32275
UniProtKB/Swiss-Prot	Q04047
UniProtKB/TrEMBL	Q8IR16
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | ubiquitous   (Rendahl et al., 1992)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | ubiquitous   (Rendahl et al., 1992)   (Frasch and Saumweber, 1989) embryonic stage 5     (Frasch and Saumweber, 1989) third instar larval stage   embryonic/larval salivary gland   (Frasch and Saumweber, 1989) pupal stage   organism | ubiquitous   (Rendahl et al., 1992) adult stage & oogenesis   nurse cell   (Frasch and Saumweber, 1989) follicle cell   (Frasch and Saumweber, 1989) oocyte   (Frasch and Saumweber, 1989) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 5   organism | ubiquitous & uniform   • cytoplasm (Rendahl et al., 1992) larval stage   organism | ubiquitous   (Rendahl et al., 1992) late third instar larval stage   embryonic/larval brain   (Rendahl et al., 1992) ventral nerve cord   (Rendahl et al., 1992) adult stage   organism | ubiquitous   (Rendahl et al., 1992) eye photoreceptor cell   • nucleus (Rendahl et al., 1992) lamina   • nucleus (Rendahl et al., 1992) lobula complex   • nucleus (Rendahl et al., 1992) first optic chiasm   • nucleus (Rendahl et al., 1992) medulla   • nucleus (Rendahl et al., 1992) adult central complex   • nucleus (Rendahl et al., 1992) cortex region of thoracico-abdominal ganglion   • nucleus (Rendahl et al., 1992) adult gut   • nucleus (Rendahl et al., 1992) skeletal muscle of thorax   • nucleus (Rendahl et al., 1992) adult stage & oogenesis   oocyte | ubiquitous   (Rendahl et al., 1992) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- larval stage   organism   (Rendahl et al., 1992) adult stage | male   head   (Rendahl et al., 1992) adult stage | female   head   (Rendahl et al., 1992)
Additional Descriptive Data
	nonA protein is distributed ubiquitously in oocytes, embryos, larvae, pupae, and adults. (Rendahl et al., 1992) During oogenesis, observed in nuclei of follicle cell, nurse cells, and the oocyte. In embryos, observed in nuclei of cells at cellular blastoderm; expressed throughout embryonic development. In larval salivary glands, localized to polytene chromosomes. (Buchenau et al., 1993, Frasch and Saumweber, 1989)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) nucleus inferred from direct assay (Buchenau et al., 1993, Rendahl et al., 1992) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    37 central nervous system, pupae P8    28 head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3  (37) central nervous system, pupae P8  (28) head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    37 central nervous system, pupae P8    28 head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    37 central nervous system, pupae P8    28 head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    37 central nervous system, pupae P8    28 head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3  (37) central nervous system, pupae P8  28 head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    37 central nervous system, pupae P8    28 head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    37 central nervous system, pupae P8    28 head, virgin 1-day female    12 head, virgin 4-day female    11 head, virgin 20-day female    7 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    14 head, mated 4-day male    17 head, mated 20-day male    15 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    5 fat body, white prepupae    15 fat body, pupae P8    16 carcass, larvae L3 wandering    24 carcass, 1-day adult    5 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    17 ovary, mated 4-day female    19 testis, mated 4-day male    3 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    32 embryo 04-06hr    42 embryo 06-08hr    44 embryo 08-10hr    42 embryo 10-12hr    31 embryo 12-14hr    29 embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr  (32) embryo 04-06hr  (42) embryo 06-08hr  (44) embryo 08-10hr  (42) embryo 10-12hr  (31) embryo 12-14hr  (29) embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    32 embryo 04-06hr    42 embryo 06-08hr    44 embryo 08-10hr    42 embryo 10-12hr    31 embryo 12-14hr    29 embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    32 embryo 04-06hr    42 embryo 06-08hr    44 embryo 08-10hr    42 embryo 10-12hr    31 embryo 12-14hr    29 embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    32 embryo 04-06hr    42 embryo 06-08hr    44 embryo 08-10hr    42 embryo 10-12hr    31 embryo 12-14hr    29 embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr  32 embryo 04-06hr  (42) embryo 06-08hr  (44) embryo 08-10hr  (42) embryo 10-12hr  31 embryo 12-14hr  29 embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    32 embryo 04-06hr    42 embryo 06-08hr    44 embryo 08-10hr    42 embryo 10-12hr    31 embryo 12-14hr    29 embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    32 embryo 04-06hr    42 embryo 06-08hr    44 embryo 08-10hr    42 embryo 10-12hr    31 embryo 12-14hr    29 embryo 14-16hr    23 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    12 embryo 22-24hr    12 larva L1    7 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    18 white prepupae new    22 white prepupae 12hr    18 white prepupae 24hr    24 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    9 adult female 05day    13 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    10 embryonic 1182-4H    15 embryonic GM2    10 embryonic Kc167    10 embryonic S1    12 embryonic S3    21 leg disc CME L1    10 wing disc CME-W2    9 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    12 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    13 ovary fGS/OSS    23 ovary OSC    20 ovary OSS    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004227 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4211-RB;CG4211-RA;CG4211-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal nonA211S12 lethal, with Scer\GAL4Mef2.PR nonAGD11253 nonAGD15594 lethal | partially nonAE442A.F423A nonAK337A.F339A nonAR548C nonAR548K nonAY305A.N308A viable nonA+t11 nonAf00870 nonAS381A.N382A viable, with Scer\GAL4pnr-MD237 nonAGD11253 Sterility Allele fertile nonAf00870 Other Phenotypes Allele behavior defective nonA5 nonA9 nonA9e2-10 nonARRM1-RNP2 courtship behavior defective nonA9 nonA211S12 nonAE442A.F423A nonAK337A.F339A nonAR548C nonAR548K nonAY305A.N308A courtship behavior defective | male nonA9 courtship behavior defective | male | recessive nonA9 flight defective, with Scer\GAL4elav.PLu nonAGD11253 nonAGD15594 locomotor behavior defective, with Scer\GAL4elav.PLu nonAGD11253 nonAGD15594 neurophysiology defective nonA3 optomotor response defective nonA9 song defective nonA9 visual behavior defective nonA2 nonA5 nonA9 nonA9e2-10 nonA211S12 nonAE442A.F423A nonAK337A.F339A nonAR548C nonAR548K nonAY305A.N308A wild-type nonA+t11 nonA+tXhR11 nonAhs.PR Phenotype manifest in Allele
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of nonA Class Mutagen Stocks Known lesion nonA4b18 X ray 2 Yes nonAf00870 piggyBac transposase 2 -- nonA9 amorphic allele - genetic evidence, hypomorphic allele - genetic evidence ethyl nitrosourea 1 Yes nonAf07992 piggyBac transposase 1 -- nonA9e2-10 amorphic allele - genetic evidence X ray 0 Yes nonA1 0 -- nonA2 loss of function allele, hypomorphic allele - genetic evidence 0 Yes nonA3 hypomorphic allele - genetic evidence 0 -- nonA4 hypomorphic allele - genetic evidence 0 -- nonA5 loss of function allele, hypomorphic allele - genetic evidence 0 Yes nonA6 0 -- nonA7 0 -- nonA8 0 -- nonACPTI002223 piggyBac transposase 0 Yes nonACPTI003091 piggyBac transposase 0 Yes nonAunspecified 0 --
Alleles Carried on Transgenic Constructs ( 26 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nonA Class Mutagen Stocks Known lesion nonAGD11253 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes nonAGD15594 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nonAKK108107 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nonA+t11 in vitro construct - genomic fragment 0 Yes nonA+t16 in vitro construct - genomic fragment 0 Yes nonA+tXhR11 in vitro construct - genomic fragment 0 Yes nonA1-STOP in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes nonA2-STOP in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes nonA211S12 in vitro construct - deletion 0 Yes nonA235B12 in vitro construct - deletion 0 Yes nonA235R11-SacII in vitro construct 0 Yes nonAE442A.F423A in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonAhs.PR in vitro construct - regulatory fusion 0 Yes nonAK337A.F339A in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonANIG.4211R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes nonAR548C in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonAR548K in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonARRM1-RNP1+2 in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonARRM1-RNP2 in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonARRM2-RNP1+2 in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonAS381A.N382A in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonAScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes nonAstopI in vitro construct - amino acid replacement 0 Yes nonAstopII in vitro construct - amino acid replacement 0 Yes nonAY305A.N308A in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes nonAΔ2bp in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)4b18 (Thackeray et al., 1998, Stanewsky et al., 1993) Df(1)E150 (Kulkarni et al., 1988, Jones and Rubin, 1990) Df(1)l32 (Kulkarni et al., 1988, Stanewsky et al., 1993, Banga et al., 1995) Df(1)r75c (Jones and Rubin, 1990) Df(1)XR5 (Stanewsky et al., 1993) Df(1)XR10 (Stanewsky et al., 1993) Df(1)XR14 (Stanewsky et al., 1993) Df(1)XR38 (Stanewsky et al., 1993) Dp(1;4)80f29d (Jones and Rubin, 1990) Dp(1;4)81j23a (Jones and Rubin, 1990) Dp(1;4)81k19b (Kulkarni et al., 1988) Dp(1;4)82b6w (Kulkarni et al., 1988) Dp(1;4)82c3k (Kulkarni et al., 1988) Dp(1;4)exd81h24b (Jones and Rubin, 1990) T(1;4)9e2-10 (Stanewsky et al., 1993, Rendahl et al., 1996)
Duplicated in	Dp(1;2)r+75c (Kulkarni et al., 1988, Banga et al., 1995) Dp(1;4)80f18c (Banga et al., 1995) Dp(1;4)81l8g (Banga et al., 1995) Dp(1;4)81l12h (Banga et al., 1995) Dp(1;4)r+l (Kulkarni et al., 1988)
Not disrupted in	Df(1)6b4 (Stanewsky et al., 1993) Df(1)C246 (Kulkarni et al., 1988) Df(1)D7 (Kulkarni et al., 1988, Stanewsky et al., 1993) Df(1)g (Kulkarni et al., 1988) Df(1)KA9 (Kulkarni et al., 1988) Df(1)r-D1 (Stanewsky et al., 1993, Banga et al., 1995) Df(1)sd72b (Kulkarni et al., 1988) Df(1)XR25 (Stanewsky et al., 1993) Df(1)XR26 (Stanewsky et al., 1993) Df(1)XR53 (Stanewsky et al., 1993) Dp(1;4)80f18c (Kulkarni et al., 1988) Dp(1;4)81k21e (Jones and Rubin, 1990) Dp(1;4)81l12h (Jones and Rubin, 1990) Dp(1;Y)shi+3 (Stanewsky et al., 1993) In(1)2-4-1-1 (Kulkarni et al., 1988) In(1)D30 (Kulkarni et al., 1988)
Not duplicated in	Dp(1;3)f+71b (Kulkarni et al., 1988)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11253} NA P{GD15594} NA P{KK108107} NA P{NIG.4211R} NA P{UAS-nonA.S} NA heat-shock construct P{hsp-nonA} NA characterization construct P{1-STOP} NA P{2-STOP} NA P{132XhR11} NA P{211S12} NA P{235B12} NA P{235R11} NA P{235R11-SacII} NA P{270XS16} NA P{E442A.F423A} NA P{K337A.F339A} NA P{nonAstopI} NA P{nonAstopII} NA P{nonAΔ2bp} NA P{nonA-GAL4.Kpn1} NA P{nonA-GAL4.Kpn3} NA P{nonA-GAL4.Kpn4} NA P{nonA-GAL4.Kpn5} NA P{nonA-GAL4.Kpn7} NA P{R548C} NA P{R548K} NA P{RRM1-RNP1+2} NA P{RRM1-RNP2} NA P{RRM2-RNP1+2} NA P{S381A.N382A} NA P{Y305A.N308A} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{754.P.FSVS-0}nonACPTI003091 NA PBac{768.FSVS-0}nonACPTI002223 NA PBac{WH}nonAf00870 NA PBac{WH}nonAf07992 NA
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
male courtship behavior, veined wing generated song production inferred from mutant phenotype (Rendahl et al., 1992) visual perception inferred from mutant phenotype (Rendahl et al., 1992)
Cellular Component
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) nucleus inferred from direct assay (Buchenau et al., 1993, Rendahl et al., 1992) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) non-traceable author statement (Swiss-Prot Project Members, 1996.6.1) traceable author statement (Greenspan and Ferveur, 2000) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-) poly-pyrimidine tract binding inferred from sequence or structural similarity with NCBI_gi:4826998 (FlyBase, 1992-)
Biological Process
CV term Evidence References
male courtship behavior, veined wing generated song production non-traceable author statement (Sokolowski, 2001) traceable author statement (Greenspan and Ferveur, 2000) mRNA splicing, via spliceosome inferred by curator from GO:0071011|GO:0071013 (Herold et al., 2009) inferred from sequence or structural similarity with NCBI_gi:4826998 (FlyBase, 1992-) phototaxis non-traceable author statement (Sokolowski, 2001) visual behavior non-traceable author statement (Sokolowski, 2001) visual perception non-traceable author statement (Swiss-Prot Project Members, 1996.6.1) traceable author statement (Greenspan and Ferveur, 2000)
Cellular Component
CV term Evidence References
spliceosomal complex inferred from sequence or structural similarity with NCBI_gi:4826998 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
nonA - bl anti bait coimmunoprecipitation, western blot (Hovemann et al., 2000) nonA - lack anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nonA allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58943 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004227 DroID - A comprehensive database of gene and protein interactions. • FBgn0004227 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32603
Orthologs
OrthoDB Orthologs (62) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HHPND Dipteran inclusive ortholog search EOG6R50BJ Insect inclusive ortholog search EOG66Q5P7 Arthropod inclusive ortholog search EOG63BK4H Metazoa inclusive ortholog search EOG68WQVP
Orthologs in Drosophila Species (EOG6HHPND)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\nonA     Drosophila melanogaster fruit fly  Dmel\nonA-l     Drosophila sechellia   Dsec\GM13430 Y Y Drosophila sechellia   Dsec\GM15178   Y Drosophila erecta   Dere\GG21764   Y Drosophila erecta   Dere\GG17959 Y Y Drosophila yakuba   Dyak\GE17267   Y Drosophila yakuba   Dyak\GE24585   Y Drosophila ananassae   Dana\GF19266   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA23435   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA27079   Y Drosophila persimilis   Dper\GL23854   Y Drosophila willistoni   Dwil\GK25061 Y Y Drosophila virilis   Dvir\nonA Y Y Drosophila mojavensis   Dmoj\GI14459 Y Y Drosophila grimshawi   Dgri\GH12694 Y Y
Orthologs in non-Drosophila Dipterans (EOG6R50BJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL017116 Y Anopheles gambiae Malaria mosquito  Agam\AGAP003794 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006106 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006107 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006108 Y
Orthologs in non-Dipteran Insects (EOG66Q5P7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB54990 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009677 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11789 Y Atta cephalotes Leafcutter ant  Acep\ACEP27358 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO20132 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20046 Y Linepithema humile Argentine ant  Lhum\LH13133 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17412 Y Solenopsis invicta Red fire ant  Sinv\SINV11994 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI004173 Y Bombyx mori Silkmoth  Bmor\BGIBMGA005177 Y Pediculus humanus Human body louse  Phum\PHUM487590 Y Tribolium castaneum Red flour beetle  Tcas\TC006331 Y Tribolium castaneum Red flour beetle  Tcas\TC001621 Y
Orthologs in non-Insect Arthropods (EOG63BK4H)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_204255 Y Ixodes scapularis Deer tick  Isca\ISCW001740 Y Ixodes scapularis Deer tick  Isca\ISCW001739 Y
Orthologs in non-Arthropod Metazoa (EOG68WQVP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\psf-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015145gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027191gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027192gn Y Danio rerio Zebrafish  Drer\pspc1 Y Danio rerio Zebrafish  Drer\nono Y Danio rerio Zebrafish  Drer\sfpq Y Xenopus tropicalis Western clawed frog  Xtro\sfpq Y Xenopus tropicalis Western clawed frog  Xtro\nono Y Gallus gallus Domestic chicken  Ggal\SFPQ Y Gallus gallus Domestic chicken  Ggal\NONO Y Gallus gallus Domestic chicken  Ggal\PSPC1 Y Mus musculus House mouse  Mmus\Sfpq Y Mus musculus House mouse  Mmus\Pspc1 Y Mus musculus House mouse  Mmus\Nono Y Rattus norvegicus Norway rat  Rnor\Nono Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022823 Y Rattus norvegicus Norway rat  Rnor\Pspc1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000043047 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000030425 Y Rattus norvegicus Norway rat  Rnor\Sfpq Y Homo sapiens Human  Hsap\NONO Y Homo sapiens Human  Hsap\ENSG00000121390 Y Homo sapiens Human  Hsap\SFPQ Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\NONO     Hsap\ENSG00000121390     Hsap\SFPQ
AAA Orthologs (6) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM13430 Drosophila erecta Dere\GG17959 Drosophila willistoni Dwil\GK25061 Drosophila virilis Dvir\nonA Drosophila mojavensis Dmoj\GI14459 Drosophila grimshawi Dgri\GH12694
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	18380 w1118 PBac{WH}nonAf00870 125 Df(1)4b18, y1 cv1 v1 nonA4b18 f1 car1/FM0
Harvard	- PBac{WH}nonAf00870 - PBac{WH}nonAf07992
Kyoto	109075 nonA9 / FM7c 108891 Df(1)4b18, y1 cv1 v1 nonA4b18 f1 car1/Basc
VDRC	v100723 P{KK108107}VIE-260B v26441 w1118; P{GD11253}v26441 v26442 w1118; P{GD11253}v26442 v33901 w1118; P{GD15594}v33901/TM3
Genomic Clones ( 2 )
	BACR13G13 BACR47D09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 143 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE58280
Other clones	RH34608
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH05682 GH23247 GH27563 GM14237 GM18488 GM29292 LD03307 LD09112 LD11296 LD11715 LD15138 LD15575 LD20883 LD21122 LD21176 LD31930 LP05229 LP17816 LP19117 RE04881 RE12212 RE12943 RE13777 RE21905 RE33335 RE38232 RE38947 RE39853 RE41191 RE57110 RE68813 RH24239 RH26738 RH48264 RH52408 RH64729 RH67329 SD25948
Other clones	672 1675 17434 36883 57004 63506 63547 65892 70354 72283 74852 76037 76817 77735 78247 81537 82403 105573 105717 117782 149070 157136 161660 175344 190853 192961 194032 214211.40 214349.32 214409.45 214411.40 214481.33 214543.28 273030 274448 542809 CK01.8D.H10 CK01.14C.C10 CK01.17C.G2 CK01.21B.H1 CK01.49B.E1 CK01.83D.D2 CK01.84A.G1 CK01.94C.A2 CK01.113C.E5 CK01.145A.E11 CK01.170A.E3 EK002815 EK015342 EK041417 EK049461 EK066745 EK066925 EK104218 EK104507 EK118703 EK119318 EK121840 EK123226 EK129640 EK143609 EK148222 EK170326 EK189325 EK190319 EK193156 EK194705 EK201156 EK204943 EK207705 EK210059 EK215039 EK230855 EK237760 EK244129 EK254710 EK260557 EK265825 EK267103 EK269907 EK276464 EK296340 EK300019 EK302037 EN11357 EP08745 EP09910 EP10734 EP11106 EP11950 EP21218 FGM315C02 IP17308 RP004028648 RP004041443 RP004111396 RP004200690 RP004217704 RP004227447 RP004235858 RP004250715 RP004397968 RP004403760
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004227 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32603
Antibody Information
	monoclonal (Frasch and Saumweber, 1989) polyclonal (Rendahl et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Dissection of the functional domains of nonA. (Rendahl et al., 1996) "Gene order: In direction of increasing cytology: Cyp1- nonA?" was stated as revision. (Rutherford, 1996.11.4) The RRM motifs of nonA have been analysed by in vitro mutagenesis. RRM1 mutants cause optomotor blindness and an absence of transient spikes in the ERG. RRM2 mutants cause mildly mutant song pulses and trains. (Stanewsky et al., 1996) Spatial distribution of nonA protein was followed by confocal laser scanning microscopy in the nuclei of living cells throughout embryogenesis. Active uptake of nonA protein from cytoplasm to nucleus was observed from nuclear cycle 14 onwards. Significant differences between the distribution in fixed and living embryos were noted. The nonA protein was localized in the nuclei of living embryos at discrete sites, mostly at the periphery and sometimes tightly clustered. Observations suggest that specific protein complexes are mobile and associate with active interphase chromatin. (Buchenau et al., 1993) A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs. (Kim and Baker, 1993) Germline clonal analysis demonstrates that nonA has an essential function in the female germline. (Stanewsky et al., 1993) Murine NonO is homologous to nonA, essential for visual processing. (Yang et al., 1993) Mutants exhibit defective courtship song. (Bernstein et al., 1992) Expression patterns and nonA protein structure have been determined. (Rendahl et al., 1992) nonA locus has been molecularly cloned, mRNA transcripts characterized and nonA mutant phenotypes rescued by P element mediated transformation. (Jones and Rubin, 1990) nonA binds to the chromosome within 2.5kb 5' of the start of Sgs4 transcription. (Saumweber et al., 1990) nonA has been cloned and sequenced and its expression pattern has been analysed. (von Besser et al., 1990) Aberrant intervals between song pulses were observed in nonA mutant and cac, nonA double mutant songs. Males displayed a defect in song hums manifested by an irregular sine wave, although the fundamental frequencies were normal. (Wheeler et al., 1989) Mosaic analysis (Hotta and Benzer, 1970) suggested that ERG defects map to photoreceptors; but too few gynandromorphs were analyzed to rule out an optic-lobe 'focus', which is more likely to be the focus for visual-movement-response abnormalities (especially those reported by Heisenberg, 1972). Two genomic clones, whose distal endpoints are just distal to nonA-rescuing DNA fragments, rescue each of two non-allelic lethals <up>l(1)14Cc and l(1)14Ca</up> that map to the same cytogenetic interval as does nonA; transformation with overlapping genomic clones indicates an order from left to right of l(1)14Cc l(1)14Ca nonA (Jones and Rubin, 1990). Both lethals complement the ERG defects of nonA5 (Jones and Rubin, 1990) and complement the song abnormality of nonA9 (Kulkarni and Hall, unpublished).   Defective optomotor responses and phototaxis, with the former being especially defective (Heisenberg, 1972; Heisenberg and Buchner, 1977; Kulkarni, Steinlauf and Hall, 1988). Poor orientation behavior in Y-maze (Bulthoff, 1982). nonA5 flies exhibit specific lack of responses to front-to-back moving visual stimuli, whereas reaction to back-to-front motion is intact (Heisenberg, 1972). Physiologically, there are reduced or absent light-on and light-off transient spikes in electroretinogram, whereas photoreceptor potential is normal (Hotta and Benzer, 1970; Pak, Grossfield and Arnold, 1970; Heisenberg, 1971; Kulkarni, Steinlauf and Hall, 1988; Jones and Rubin, 1990). Larval visual response (re negative phototaxis) normal (Hotta and Keng, 1984). Courtship song is abnormal, as influenced by one, possibly two, mutant alleles; nonA9 (originally diss) males produce abnormal song, regarding pulses relatively late in trains of such song sounds (each one resulting from a bout of wing vibration); the abnormalities are polycyclicity (Kulkarni, Steinlauf and Hall, 1988) and anomalous intra-pulse frequency components (Wheeler et al., 1989); pulses early in trains, or throughout short ones, are nearly normal; courtship hum sounds manifest irregular sine waves, though their fundamental frequencies are normal (Wheeler et al., 1989). nonA9 by itself is also an optomotor-defective/ERG-abnormal mutant (Kulkarni et al., 1988) and fails to complement other mutant alleles with regard to these visual phenotypes (Rendahl, Jones, Kulkarni, Bagully and Hall, 1992); but these heterozygotes, in a homozygous tra genetic background, sing normally (with the possible exception of nonA2/nonA9, Rendahl et al., 1992), as do males hemizygous for alleles isolated as visual mutants.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078643 NM_167505 NM_001014747 RefSeq (Proteins) • NP_523367 NP_727962 NP_001014747 DDBJ / EMBL / GenBank • AA735332 AAA03214 AAA03215 AAF48597 AAF48597.2 AAM50305 AAN09398 AAN09398.1 AAX52501 AI946267 AJ296020 AY119651 BI371402 CAA39395 CZ490682 M33496 X55902 Z31966 Z32275 Entrez Gene - A searchable database of RefSeq genes. • 32603 UniProtKB/Swiss-Prot • Q04047 UniProtKB/TrEMBL • Q8IR16
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677) NOPS (IPR012975)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58943 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004227 DroID - A comprehensive database of gene and protein interactions. • FBgn0004227 DRSC - Results from RNAi screens. • FBgn0004227 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004227 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4211-RB;CG4211-RA;CG4211-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004227 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32603 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32603 modMine - Data generated by the modENCODE project. • FBgn0004227
Synonyms & Secondary IDs ( 20 )
Reported As
Symbol Synonym	Bj6 (Lehmann and Korge, 1995, Saumweber et al., 1990, von Besser et al., 1990, Herold et al., 2009) Bj6-1 (Kim and Baker, 1993) CG4211 (Peifer, 2002.5.14, Mohammad et al., 2009, Larschan et al., 2007, Keleman et al., 2009.8.5, Herold et al., 2009, Zhan et al., 2011) diss (Billeter et al., 2002, Yamamoto and Nakano, 1999, Yamamoto and Nakano, 1998, Yamamoto et al., 1997, Hall, 1994, Wheeler et al., 1989, Kulkarni et al., 1988, Mohammad et al., 2009, Hall, 2003, Kulkarni et al., 1988) nonA (Huttunen et al., 2002, Legube et al., 2006, Hall, 2002, Vicente-Crespo et al., 2008, Dietzl et al., 2007, Lindsley and Zimm, 1992, Hall, 2003, Ruedi and Hughes, 2009, Mosca and Schwarz, 2010, Gleason, 2005, Japanese National Institute of Genetics, 2012.5.21) non-A (Campesan et al., 2001, Orgad et al., 1989, Houseley et al., 2005, Jepson and Reenan, 2009) NonA (Reim et al., 1999, Onorati et al., 2011, Wang et al., 2005, Kiesler et al., 2003, Hurt et al., 2009, Hessle et al., 2009, Kozlova et al., 2006, Singh and Lakhotia, 2012) nonA/diss (Greenspan and Ferveur, 2000) opm2   p54nrb (Wang et al., 2005) x14
Name Synonym	Bj6-monoclonal-antibody   dissonance (Kulkarni et al., 1988, Hall, 2003) non-on-transient-A (Hall, 2003) no on or off transient A   no on or off transient-A (Lindsley and Zimm, 1992) no-on-transient A (Huttunen et al., 2002, Sambandan et al., 2008, Fitzpatrick, 2004, Jones, 1993.11.10) no-on transient A (Saumweber, 1990.9.12, Gleason, 2005) no-on-transient-A (Hall, 2002) Positive spike II group
Secondary FlyBase IDs
	FBgn0000188 FBgn0002957
References ( 125 )
Generate a list of	All references relating to this gene ( 125 )
List References by type	Research paper  ( 69 ) Supplementary material  ( 5 ) Review  ( 14 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 16 ) Abstract  ( 6 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 5 ) Book  ( 1 ) Stock list  ( 1 ) Thesis  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 2 ) Letter  ( 1 )
Recent research papers ( 4 )
	Dwivedi et al., 2012, PLoS ONE 7(5): e37113 In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications. [FBrf0218341] Singh and Lakhotia, 2012, J. Biosci., Bangalore 37(4): 659--678 The hnRNP A1 homolog Hrp36 is essential for normal development, female fecundity, omega speckle formation and stress tolerance in Drosophila melanogaster. [FBrf0219285] Onorati et al., 2011, PLoS Genet. 7(5): e1002096 The ISWI Chromatin Remodeler Organizes the hsrω ncRNA–Containing Omega Speckle Nuclear Compartments. [FBrf0213856] Zhan et al., 2011, Gene 470(1-2): 1--6 Evolution of alternative splicing in newly evolved genes of Drosophila. [FBrf0212278] Show all research papers ...
Recent reviews ( 1 )
	Lakhotia, 2012, Wiley Interdiscip. Rev. RNA 3(6): 779--796 Long non-coding RNAs coordinate cellular responses to stress. [FBrf0219838] Show all reviews ...