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Gene Dmel\Hrb87F

General Information
Symbol	Dmel\Hrb87F	Species	D. melanogaster
Name	Heterogeneous nuclear ribonucleoprotein at 87F	Annotation symbol	CG12749
Feature type	protein_coding_gene	FlyBase ID	FBgn0004237
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	hrp36, P11
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	87F7-87F7	Sequence location	3R:9,482,568..9,486,253 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heterogeneous nuclear ribonucleoprotein at 87F is referred to in FlyBase by the symbol Dmel\Hrb87F (CG12749, FBgn0004237). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding. There is experimental evidence that it is involved in the biological process: mitosis; regulation of alternative mRNA splicing, via spliceosome; compound eye morphogenesis; neurogenesis. 17 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, adult testis, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639118_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Hrb87F. Gene sequence location is 3R:9482568..9486253.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hrb2: hnRNA-binding protein 2 (S. Haynes) Homology to the mammalian A and B hnRNP proteins based on amino acid sequence inferred from nucleotide sequence.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87F7-87F7   Limits computationally determined from genome sequence between P{PZ}CtBP03463 and P{lacW}B52s2249&P{lacW}flflL4179
Experimentally Determined Cytological Location
Cytogenetic map Notes References 85C-85D     (Hovemann, 1991.2.28) 87F-87F   (determined by in situ hybridisation)   (Matunis et al., 1992) 87F-87F   (determined by in situ hybridisation)   (Haynes et al., 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hrb87F for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.47 Low-frequency RNA-Seq exon junction(s) not annotated. DGC clone GH05625 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hrb87F-RA FBtr0082851  NM_057459   2281   385 Hrb87F-RC FBtr0300590  NM_001170131   1810   385 Hrb87F-RD FBtr0310621  NM_001275609   1587   385 Hrb87F-RE FBtr0334495  NM_057458   2759   385
Additional Transcript Data & Comments
Reported size (kB)	2.3, 1.8 (compiled cDNA) (Hovemann et al., 1991) 2.2, 1.7 (northern blot) (Haynes et al., 1991) 1.1 (longest cDNA) (Matunis et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hrb87F-PA   FBpp0082316   39.5   385   9.46     NP_476807   AAF54967 Hrb87F-PC   FBpp0289817   39.5   385   9.46     NP_001163602   ACZ94899 Hrb87F-PD   FBpp0302741   39.5   385   9.46     NP_001262538   AGB95920 Hrb87F-PE   FBpp0306562   39.5   385   9.46     NP_476806   AAN13574
Additional Polypeptide Data & Comments
Reported size (kDa)	386 (aa); 40 (kD predicted) (Haynes et al., 1991) 386 (aa); 38 (kD observed); 40 (kD predicted) (Hovemann et al., 1991) 326 (aa); 34 (kD observed); 34 (kD predicted) (Matunis et al., 1992) 36 (kD observed) (Matunis et al., 1992)
Comments	One of a couple of protein products. (FlyBase, 1992-, FlyBase, 1992-) hnRNP-specific mAbs were obtained and used to purify hnRNP complexes from S2 cells. hnRNP proteins were identified on the basis of nucleoplasmic localization, association with nascent transcripts, crosslinking to poly(A)-containing RNA in living cells, and amino acid sequence. Staining of polytene chromosomes reveals that Hrb87F protein is associated with actively transcribing loci. (Matunis et al., 1992) Hrb87F protein has two amino-terminal RNP-CS RNA-binding domains and a carboxy-terminal glycine-rich auxiliary domain. (Matunis et al., 1992) Staining of polytene chromosomes with antibody against Hrb87F protein reveals a unique banding pattern. (Hovemann et al., 1991)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201135   AA201547   AA202812   AA246324   AA264275   AA540341   AA735103   AA801903   AA802948   AA820273   AA942284   AA949001   AA949288   AA949883   AA949982   AA950457   AA951216   AA978720   AA978730   AE014297 AAF54967   AE014297 AAN13574   AC007646   AE014297 ACZ94899   AE014297 AGB95920   AI107775   AI238813   AI297345   AI297577   AI403482   AI406171   AI456731   AI512455   AI514449   AI515942   AI516442   AI518330   AI519398   AI945591   BE978416   BF485586   BF504861   BG634489   BH854519   BH854520   BI165340   BI166019   BI170721   BI171678   BI173260   BI173553   BI215933   BI227925   BI228786   BI231042   BI352515   BI352701   BI354215   BI363058   BI363792   BI365356   BI374233   BI374585   BI481748   BI482932   BI484335   BI578809   BI579193   BI580372   BI632985   BI636885   BI637908   BI639519   BI641129   BT011153   BT012315   BT126124   CK661959   CK662214   CK662374   CO186935   CO191224   CO267120   CO271753   CO274789   CO285213   CO292383   CO292536   CO294131   CO297347   CO301332   CO306609   CO310712   CO311979   CO319608   CO325594   CO329856   CO330688   CO332495   DN153991   EC066970   EC078875   EC083571   EC084414   EC087863   EC089875   EC091179   EC194910   EC196403   EC199086   EC199997   EC201878   EC202249   EC203053   EC211202   EC212787   EC227934   EC232089   EC232798   EC232937   EC235427   EC235842   EC236631   EC246949   EC247032   EC250546   EC251145   ES690563   ES690565   ES690567   ES690577   ES690598   ES690614   ES692939   EV577008   EV579218   EV592423   EV598101   GH941151   GH944497   GH945419   GH945689   GH954130   GH958160   GH962368   GH962641   GH969540   GH977779   GH978692   GH978959   GH979040   GH980122   GH980336   GH983972   X54803 CAA38574   X58183 CAA41170   X58184   X59691 CAA42212   X62636 CAA44502
UniProtKB/Swiss-Prot	P48810
UniProtKB/TrEMBL	E1JIK0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Haynes et al., 1991) oogenesis & adult stage | female   ovary   (Haynes et al., 1991)
Additional Descriptive Data
	2.3kb Hrb87F transcripts are expressed throughout development. Transcripts are abundant in ovaries and early embryos, decline in late embryos and early larvae, and then increase again from the second larval instar stage. (Haynes et al., 1991)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
chromatin inferred from direct assay (Prasanth et al., 2000) nucleoplasm inferred from direct assay (Matunis et al., 1992) nucleus inferred from direct assay (Prasanth et al., 2000) omega speckle inferred from direct assay (Prasanth et al., 2000) polytene chromosome puff inferred from direct assay (Buszczak and Spradling, 2006, Matunis et al., 1992) ribonucleoprotein complex inferred from direct assay (Matunis et al., 1992) inferred from physical interaction with sqd (Matunis et al., 1992)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, adult testis, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1389.725 Larval Midgut    436.5 Larval Hindgut    668.7 Larval Malpighian Tubules    992.1 Larval Fat Body    498.8 Larval Salivary Gland    1093.6 Larval Trachea    847.75 Larval Carcass    950.925 Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland    594.1 Adult Heart    345.075 Adult VirginFemale Spermatheca    833.6 Adult InseminatedFemale Spermatheca    868.3 Adult Ovary    913.9 Adult Testis    126.9 Adult Male Accessory Gland    544.7 Adult Carcass    261.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1389.725) Larval Midgut    436.5 Larval Hindgut  (668.7) Larval Malpighian Tubules  (992.1) Larval Fat Body    498.8 Larval Salivary Gland  (1093.6) Larval Trachea  (847.75) Larval Carcass  (950.925) Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland  (594.1) Adult Heart    345.075 Adult VirginFemale Spermatheca  (833.6) Adult InseminatedFemale Spermatheca  (868.3) Adult Ovary  (913.9) Adult Testis    126.9 Adult Male Accessory Gland  (544.7) Adult Carcass    261.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1389.725) Larval Midgut    436.5 Larval Hindgut    668.7 Larval Malpighian Tubules    992.1 Larval Fat Body    498.8 Larval Salivary Gland  (1093.6) Larval Trachea    847.75 Larval Carcass    950.925 Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland    594.1 Adult Heart    345.075 Adult VirginFemale Spermatheca    833.6 Adult InseminatedFemale Spermatheca    868.3 Adult Ovary    913.9 Adult Testis    126.9 Adult Male Accessory Gland    544.7 Adult Carcass    261.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1389.725 Larval Midgut    436.5 Larval Hindgut    668.7 Larval Malpighian Tubules    992.1 Larval Fat Body    498.8 Larval Salivary Gland    1093.6 Larval Trachea    847.75 Larval Carcass    950.925 Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland    594.1 Adult Heart    345.075 Adult VirginFemale Spermatheca    833.6 Adult InseminatedFemale Spermatheca    868.3 Adult Ovary    913.9 Adult Testis    126.9 Adult Male Accessory Gland    544.7 Adult Carcass    261.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1389.725 Larval Midgut    436.5 Larval Hindgut    668.7 Larval Malpighian Tubules    992.1 Larval Fat Body    498.8 Larval Salivary Gland    1093.6 Larval Trachea    847.75 Larval Carcass    950.925 Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland    594.1 Adult Heart    345.075 Adult VirginFemale Spermatheca    833.6 Adult InseminatedFemale Spermatheca    868.3 Adult Ovary    913.9 Adult Testis    126.9 Adult Male Accessory Gland    544.7 Adult Carcass    261.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1389.725) Larval Midgut    436.5 Larval Hindgut  668.7 Larval Malpighian Tubules  (992.1) Larval Fat Body    498.8 Larval Salivary Gland  (1093.6) Larval Trachea  (847.75) Larval Carcass  (950.925) Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland  594.1 Adult Heart    345.075 Adult VirginFemale Spermatheca  (833.6) Adult InseminatedFemale Spermatheca  (868.3) Adult Ovary  (913.9) Adult Testis    126.9 Adult Male Accessory Gland  544.7 Adult Carcass    261.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1389.725 Larval Midgut    436.5 Larval Hindgut    668.7 Larval Malpighian Tubules    992.1 Larval Fat Body    498.8 Larval Salivary Gland  1093.6 Larval Trachea    847.75 Larval Carcass    950.925 Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland    594.1 Adult Heart    345.075 Adult VirginFemale Spermatheca    833.6 Adult InseminatedFemale Spermatheca    868.3 Adult Ovary    913.9 Adult Testis    126.9 Adult Male Accessory Gland    544.7 Adult Carcass    261.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1389.725 Larval Midgut    436.5 Larval Hindgut    668.7 Larval Malpighian Tubules    992.1 Larval Fat Body    498.8 Larval Salivary Gland    1093.6 Larval Trachea    847.75 Larval Carcass    950.925 Adult Head    288.3 Adult Eye    337.125 Adult Brain    371.4 Adult Thoracic-Abdominal Ganglion    405 Adult Crop    406.1 Adult Midgut    334.9 Adult Hindgut    353.4 Adult Malpighian Tubules    300.5 Adult Fat Body    379.1 Adult Salivary Gland    594.1 Adult Heart    345.075 Adult VirginFemale Spermatheca    833.6 Adult InseminatedFemale Spermatheca    868.3 Adult Ovary    913.9 Adult Testis    126.9 Adult Male Accessory Gland    544.7 Adult Carcass    261.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    288.3   NA  Eye    337.125   NA  Brain    371.4   1389.725  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    405   NA  Crop    406.1   436.5  Midgut    334.9   668.7  Hindgut    353.4   992.1  Malpighian Tubules    300.5   498.8  Fat Body    379.1   1093.6  Salivary Gland    594.1   NA  Heart    345.075   847.75  Trachea    NA   NA  VirginFemale Spermatheca    833.6   NA  InseminatedFemale Spermatheca    868.3   NA  Ovary    913.9   NA  Testis    126.9   NA  Male Accessory Gland    544.7   950.925  Carcass    261.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    157 central nervous system, larvae L3    180 central nervous system, pupae P8    45 head, virgin 1-day female    25 head, virgin 4-day female    26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female    30 head, mated 20-day female    26 head, mated 1-day male    24 head, mated 4-day male    40 head, mated 20-day male    56 salivary gland, larvae L3 wandering    44 salivary gland, white prepupae    169 digestive system, larvae L3 wandering    58 digestive system, 1-day adult    27 digestive system, 4-day adult    47 digestive system, 20-day adult    31 fat body, larvae L3 wandering    22 fat body, white prepupae    71 fat body, pupae P8    50 carcass, larvae L3 wandering    176 carcass, 1-day adult    22 carcass, 4-day adult    36 carcass, 20-day adult    24 ovary, virgin 4-day female    165 ovary, mated 4-day female    184 testis, mated 4-day male    47 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (157) central nervous system, larvae L3  (180) central nervous system, pupae P8  (45) head, virgin 1-day female    25 head, virgin 4-day female  26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female  (30) head, mated 20-day female  26 head, mated 1-day male    24 head, mated 4-day male  (40) head, mated 20-day male  (56) salivary gland, larvae L3 wandering  (44) salivary gland, white prepupae  (169) digestive system, larvae L3 wandering  (58) digestive system, 1-day adult  (27) digestive system, 4-day adult  (47) digestive system, 20-day adult  (31) fat body, larvae L3 wandering    22 fat body, white prepupae  (71) fat body, pupae P8  (50) carcass, larvae L3 wandering  (176) carcass, 1-day adult    22 carcass, 4-day adult  (36) carcass, 20-day adult    24 ovary, virgin 4-day female  (165) ovary, mated 4-day female  (184) testis, mated 4-day male  (47) accessory gland, mated 4-day male  (32) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (157) central nervous system, larvae L3  (180) central nervous system, pupae P8    45 head, virgin 1-day female    25 head, virgin 4-day female    26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female    30 head, mated 20-day female    26 head, mated 1-day male    24 head, mated 4-day male    40 head, mated 20-day male    56 salivary gland, larvae L3 wandering    44 salivary gland, white prepupae  (169) digestive system, larvae L3 wandering    58 digestive system, 1-day adult    27 digestive system, 4-day adult    47 digestive system, 20-day adult    31 fat body, larvae L3 wandering    22 fat body, white prepupae    71 fat body, pupae P8    50 carcass, larvae L3 wandering  (176) carcass, 1-day adult    22 carcass, 4-day adult    36 carcass, 20-day adult    24 ovary, virgin 4-day female  (165) ovary, mated 4-day female  (184) testis, mated 4-day male    47 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    157 central nervous system, larvae L3    180 central nervous system, pupae P8    45 head, virgin 1-day female    25 head, virgin 4-day female    26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female    30 head, mated 20-day female    26 head, mated 1-day male    24 head, mated 4-day male    40 head, mated 20-day male    56 salivary gland, larvae L3 wandering    44 salivary gland, white prepupae    169 digestive system, larvae L3 wandering    58 digestive system, 1-day adult    27 digestive system, 4-day adult    47 digestive system, 20-day adult    31 fat body, larvae L3 wandering    22 fat body, white prepupae    71 fat body, pupae P8    50 carcass, larvae L3 wandering    176 carcass, 1-day adult    22 carcass, 4-day adult    36 carcass, 20-day adult    24 ovary, virgin 4-day female    165 ovary, mated 4-day female    184 testis, mated 4-day male    47 accessory gland, mated 4-day male    32 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    157 central nervous system, larvae L3    180 central nervous system, pupae P8    45 head, virgin 1-day female    25 head, virgin 4-day female    26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female    30 head, mated 20-day female    26 head, mated 1-day male    24 head, mated 4-day male    40 head, mated 20-day male    56 salivary gland, larvae L3 wandering    44 salivary gland, white prepupae    169 digestive system, larvae L3 wandering    58 digestive system, 1-day adult    27 digestive system, 4-day adult    47 digestive system, 20-day adult    31 fat body, larvae L3 wandering    22 fat body, white prepupae    71 fat body, pupae P8    50 carcass, larvae L3 wandering    176 carcass, 1-day adult    22 carcass, 4-day adult    36 carcass, 20-day adult    24 ovary, virgin 4-day female    165 ovary, mated 4-day female    184 testis, mated 4-day male    47 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (157) central nervous system, larvae L3  (180) central nervous system, pupae P8  (45) head, virgin 1-day female    25 head, virgin 4-day female  26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female  30 head, mated 20-day female  26 head, mated 1-day male    24 head, mated 4-day male  (40) head, mated 20-day male  (56) salivary gland, larvae L3 wandering  (44) salivary gland, white prepupae  (169) digestive system, larvae L3 wandering  (58) digestive system, 1-day adult  27 digestive system, 4-day adult  (47) digestive system, 20-day adult  31 fat body, larvae L3 wandering    22 fat body, white prepupae  (71) fat body, pupae P8  (50) carcass, larvae L3 wandering  (176) carcass, 1-day adult    22 carcass, 4-day adult  (36) carcass, 20-day adult    24 ovary, virgin 4-day female  (165) ovary, mated 4-day female  (184) testis, mated 4-day male  (47) accessory gland, mated 4-day male  32 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (157) central nervous system, larvae L3  (180) central nervous system, pupae P8    45 head, virgin 1-day female    25 head, virgin 4-day female    26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female    30 head, mated 20-day female    26 head, mated 1-day male    24 head, mated 4-day male    40 head, mated 20-day male    56 salivary gland, larvae L3 wandering    44 salivary gland, white prepupae  (169) digestive system, larvae L3 wandering    58 digestive system, 1-day adult    27 digestive system, 4-day adult    47 digestive system, 20-day adult    31 fat body, larvae L3 wandering    22 fat body, white prepupae    71 fat body, pupae P8    50 carcass, larvae L3 wandering  (176) carcass, 1-day adult    22 carcass, 4-day adult    36 carcass, 20-day adult    24 ovary, virgin 4-day female  (165) ovary, mated 4-day female  (184) testis, mated 4-day male    47 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    157 central nervous system, larvae L3    180 central nervous system, pupae P8    45 head, virgin 1-day female    25 head, virgin 4-day female    26 head, virgin 20-day female    20 head, mated 1-day female    24 head, mated 4-day female    30 head, mated 20-day female    26 head, mated 1-day male    24 head, mated 4-day male    40 head, mated 20-day male    56 salivary gland, larvae L3 wandering    44 salivary gland, white prepupae    169 digestive system, larvae L3 wandering    58 digestive system, 1-day adult    27 digestive system, 4-day adult    47 digestive system, 20-day adult    31 fat body, larvae L3 wandering    22 fat body, white prepupae    71 fat body, pupae P8    50 carcass, larvae L3 wandering    176 carcass, 1-day adult    22 carcass, 4-day adult    36 carcass, 20-day adult    24 ovary, virgin 4-day female    165 ovary, mated 4-day female    184 testis, mated 4-day male    47 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    220 embryo 02-04hr    300 embryo 04-06hr    314 embryo 06-08hr    362 embryo 08-10hr    246 embryo 10-12hr    139 embryo 12-14hr    130 embryo 14-16hr    123 embryo 16-18hr    70 embryo 18-20hr    41 embryo 20-22hr    53 embryo 22-24hr    44 larva L1    69 larva L2    95 larva L3 12hr old    62 larva L3 puffstage 1-2    59 larva L3 puffstage 3-6    121 larva L3 puffstage 7-9    169 white prepupae new    166 white prepupae 12hr    89 white prepupae 24hr    126 pupae 2d postWPP    66 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    43 adult male 05day    64 adult male 30day    75 adult female 01day    90 adult female 05day    146 adult female 30day    147 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (220) embryo 02-04hr  (300) embryo 04-06hr  (314) embryo 06-08hr  (362) embryo 08-10hr  (246) embryo 10-12hr  (139) embryo 12-14hr  (130) embryo 14-16hr  (123) embryo 16-18hr  (70) embryo 18-20hr  (41) embryo 20-22hr  (53) embryo 22-24hr  (44) larva L1  (69) larva L2  (95) larva L3 12hr old  (62) larva L3 puffstage 1-2  (59) larva L3 puffstage 3-6  (121) larva L3 puffstage 7-9  (169) white prepupae new  (166) white prepupae 12hr  (89) white prepupae 24hr  (126) pupae 2d postWPP  (66) pupae 3d postWPP  (32) pupae 4d postWPP  (38) adult male 01day  (43) adult male 05day  (64) adult male 30day  (75) adult female 01day  (90) adult female 05day  (146) adult female 30day  (147) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (220) embryo 02-04hr  (300) embryo 04-06hr  (314) embryo 06-08hr  (362) embryo 08-10hr  (246) embryo 10-12hr  (139) embryo 12-14hr  (130) embryo 14-16hr  (123) embryo 16-18hr    70 embryo 18-20hr    41 embryo 20-22hr    53 embryo 22-24hr    44 larva L1    69 larva L2    95 larva L3 12hr old    62 larva L3 puffstage 1-2    59 larva L3 puffstage 3-6  (121) larva L3 puffstage 7-9  (169) white prepupae new  (166) white prepupae 12hr    89 white prepupae 24hr  (126) pupae 2d postWPP    66 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    43 adult male 05day    64 adult male 30day    75 adult female 01day    90 adult female 05day  (146) adult female 30day  (147) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    220 embryo 02-04hr    300 embryo 04-06hr    314 embryo 06-08hr    362 embryo 08-10hr    246 embryo 10-12hr    139 embryo 12-14hr    130 embryo 14-16hr    123 embryo 16-18hr    70 embryo 18-20hr    41 embryo 20-22hr    53 embryo 22-24hr    44 larva L1    69 larva L2    95 larva L3 12hr old    62 larva L3 puffstage 1-2    59 larva L3 puffstage 3-6    121 larva L3 puffstage 7-9    169 white prepupae new    166 white prepupae 12hr    89 white prepupae 24hr    126 pupae 2d postWPP    66 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    43 adult male 05day    64 adult male 30day    75 adult female 01day    90 adult female 05day    146 adult female 30day    147 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    220 embryo 02-04hr    300 embryo 04-06hr    314 embryo 06-08hr    362 embryo 08-10hr    246 embryo 10-12hr    139 embryo 12-14hr    130 embryo 14-16hr    123 embryo 16-18hr    70 embryo 18-20hr    41 embryo 20-22hr    53 embryo 22-24hr    44 larva L1    69 larva L2    95 larva L3 12hr old    62 larva L3 puffstage 1-2    59 larva L3 puffstage 3-6    121 larva L3 puffstage 7-9    169 white prepupae new    166 white prepupae 12hr    89 white prepupae 24hr    126 pupae 2d postWPP    66 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    43 adult male 05day    64 adult male 30day    75 adult female 01day    90 adult female 05day    146 adult female 30day    147 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (220) embryo 02-04hr  (300) embryo 04-06hr  (314) embryo 06-08hr  (362) embryo 08-10hr  (246) embryo 10-12hr  (139) embryo 12-14hr  (130) embryo 14-16hr  (123) embryo 16-18hr  (70) embryo 18-20hr  (41) embryo 20-22hr  (53) embryo 22-24hr  (44) larva L1  (69) larva L2  (95) larva L3 12hr old  (62) larva L3 puffstage 1-2  (59) larva L3 puffstage 3-6  (121) larva L3 puffstage 7-9  (169) white prepupae new  (166) white prepupae 12hr  (89) white prepupae 24hr  (126) pupae 2d postWPP  (66) pupae 3d postWPP  32 pupae 4d postWPP  (38) adult male 01day  (43) adult male 05day  (64) adult male 30day  (75) adult female 01day  (90) adult female 05day  (146) adult female 30day  (147) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (220) embryo 02-04hr  (300) embryo 04-06hr  (314) embryo 06-08hr  (362) embryo 08-10hr  (246) embryo 10-12hr  139 embryo 12-14hr  130 embryo 14-16hr  123 embryo 16-18hr    70 embryo 18-20hr    41 embryo 20-22hr    53 embryo 22-24hr    44 larva L1    69 larva L2    95 larva L3 12hr old    62 larva L3 puffstage 1-2    59 larva L3 puffstage 3-6  121 larva L3 puffstage 7-9  (169) white prepupae new  (166) white prepupae 12hr    89 white prepupae 24hr  126 pupae 2d postWPP    66 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    43 adult male 05day    64 adult male 30day    75 adult female 01day    90 adult female 05day  (146) adult female 30day  (147) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    220 embryo 02-04hr    300 embryo 04-06hr    314 embryo 06-08hr    362 embryo 08-10hr    246 embryo 10-12hr    139 embryo 12-14hr    130 embryo 14-16hr    123 embryo 16-18hr    70 embryo 18-20hr    41 embryo 20-22hr    53 embryo 22-24hr    44 larva L1    69 larva L2    95 larva L3 12hr old    62 larva L3 puffstage 1-2    59 larva L3 puffstage 3-6    121 larva L3 puffstage 7-9    169 white prepupae new    166 white prepupae 12hr    89 white prepupae 24hr    126 pupae 2d postWPP    66 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    43 adult male 05day    64 adult male 30day    75 adult female 01day    90 adult female 05day    146 adult female 30day    147 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    187 Schneider line 2 Sg4    123 embryonic 1182-4H    166 embryonic GM2    95 embryonic Kc167    58 embryonic S1    170 embryonic S3    143 leg disc CME L1    160 wing disc CME-W2    118 wing disc ML-DmD8    133 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    180 wing disc ML-DmD21    153 wing disc ML-DmD32    157 haltere disc ML-DmD17-c3    171 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    80 mixed discs ML-DmD4-c1    168 CNS ML-DmBG1-c1    162 CNS ML-DmBG2-c2    102 tumorous blood cells mbn2    121 ovary fGS/OSS    216 ovary OSC    165 ovary OSS    192 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (187) Schneider line 2 Sg4  (123) embryonic 1182-4H  (166) embryonic GM2  (95) embryonic Kc167  (58) embryonic S1  (170) embryonic S3  (143) leg disc CME L1  (160) wing disc CME-W2  (118) wing disc ML-DmD8  (133) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (180) wing disc ML-DmD21  (153) wing disc ML-DmD32  (157) haltere disc ML-DmD17-c3  (171) eye-antennal disc ML-DmD11  (123) antennal disc ML-DmD20-c5  (80) mixed discs ML-DmD4-c1  (168) CNS ML-DmBG1-c1  (162) CNS ML-DmBG2-c2  (102) tumorous blood cells mbn2  (121) ovary fGS/OSS  (216) ovary OSC  (165) ovary OSS  (192) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (187) Schneider line 2 Sg4  (123) embryonic 1182-4H  (166) embryonic GM2    95 embryonic Kc167    58 embryonic S1  (170) embryonic S3  (143) leg disc CME L1  (160) wing disc CME-W2  (118) wing disc ML-DmD8  (133) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (180) wing disc ML-DmD21  (153) wing disc ML-DmD32  (157) haltere disc ML-DmD17-c3  (171) eye-antennal disc ML-DmD11  (123) antennal disc ML-DmD20-c5    80 mixed discs ML-DmD4-c1  (168) CNS ML-DmBG1-c1  (162) CNS ML-DmBG2-c2  102 tumorous blood cells mbn2  (121) ovary fGS/OSS  (216) ovary OSC  (165) ovary OSS  (192) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    187 Schneider line 2 Sg4    123 embryonic 1182-4H    166 embryonic GM2    95 embryonic Kc167    58 embryonic S1    170 embryonic S3    143 leg disc CME L1    160 wing disc CME-W2    118 wing disc ML-DmD8    133 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    180 wing disc ML-DmD21    153 wing disc ML-DmD32    157 haltere disc ML-DmD17-c3    171 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    80 mixed discs ML-DmD4-c1    168 CNS ML-DmBG1-c1    162 CNS ML-DmBG2-c2    102 tumorous blood cells mbn2    121 ovary fGS/OSS    216 ovary OSC    165 ovary OSS    192 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    187 Schneider line 2 Sg4    123 embryonic 1182-4H    166 embryonic GM2    95 embryonic Kc167    58 embryonic S1    170 embryonic S3    143 leg disc CME L1    160 wing disc CME-W2    118 wing disc ML-DmD8    133 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    180 wing disc ML-DmD21    153 wing disc ML-DmD32    157 haltere disc ML-DmD17-c3    171 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    80 mixed discs ML-DmD4-c1    168 CNS ML-DmBG1-c1    162 CNS ML-DmBG2-c2    102 tumorous blood cells mbn2    121 ovary fGS/OSS    216 ovary OSC    165 ovary OSS    192 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (187) Schneider line 2 Sg4  (123) embryonic 1182-4H  (166) embryonic GM2  (95) embryonic Kc167  (58) embryonic S1  (170) embryonic S3  (143) leg disc CME L1  (160) wing disc CME-W2  (118) wing disc ML-DmD8  (133) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (180) wing disc ML-DmD21  (153) wing disc ML-DmD32  (157) haltere disc ML-DmD17-c3  (171) eye-antennal disc ML-DmD11  (123) antennal disc ML-DmD20-c5  (80) mixed discs ML-DmD4-c1  (168) CNS ML-DmBG1-c1  (162) CNS ML-DmBG2-c2  (102) tumorous blood cells mbn2  (121) ovary fGS/OSS  (216) ovary OSC  (165) ovary OSS  (192) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (187) Schneider line 2 Sg4  123 embryonic 1182-4H  (166) embryonic GM2    95 embryonic Kc167    58 embryonic S1  (170) embryonic S3  143 leg disc CME L1  (160) wing disc CME-W2  118 wing disc ML-DmD8  133 wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (180) wing disc ML-DmD21  (153) wing disc ML-DmD32  (157) haltere disc ML-DmD17-c3  (171) eye-antennal disc ML-DmD11  123 antennal disc ML-DmD20-c5    80 mixed discs ML-DmD4-c1  (168) CNS ML-DmBG1-c1  (162) CNS ML-DmBG2-c2  102 tumorous blood cells mbn2  121 ovary fGS/OSS  (216) ovary OSC  (165) ovary OSS  (192) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    187 Schneider line 2 Sg4    123 embryonic 1182-4H    166 embryonic GM2    95 embryonic Kc167    58 embryonic S1    170 embryonic S3    143 leg disc CME L1    160 wing disc CME-W2    118 wing disc ML-DmD8    133 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    180 wing disc ML-DmD21    153 wing disc ML-DmD32    157 haltere disc ML-DmD17-c3    171 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    80 mixed discs ML-DmD4-c1    168 CNS ML-DmBG1-c1    162 CNS ML-DmBG2-c2    102 tumorous blood cells mbn2    121 ovary fGS/OSS    216 ovary OSC    165 ovary OSS    192 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    96 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    34 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    71 Cadmium 100 mM 48 hrs, 4-day adult    118 Copper 0.5 mM 12 hrs, larvae L3    72 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    79 Zinc 4.5 mM 48 hrs, 4-day adult    70 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    31 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    113 Paraquat 5 mM 48 hrs, 4-day adult    103 Paraquat 10 mM 48 hrs, 4-day adult    80 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (119) cold shock, 4-day adult  (96) heat shock, 4-day adult  (74) Cadmium 50 mM 6 hrs, larvae L3  (34) Cadmium 50 mM 12 hrs, larvae L3  (44) Cadmium 50 mM 48 hrs, 4-day adult  (71) Cadmium 100 mM 48 hrs, 4-day adult  (118) Copper 0.5 mM 12 hrs, larvae L3  (72) Copper 15 mM 48 hrs, 4-day adult  (104) Zinc 5 mM 12 hrs, larvae L3  (79) Zinc 4.5 mM 48 hrs, 4-day adult  (70) Ethanol 2.5% 3 hrs, larvae L3  (110) Ethanol 5% 3 hrs, larvae L3  (31) Ethanol 10% 3 hrs, larvae L3  (35) Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (102) Caffeine 25 mg/ml 48 hrs, 4-day adult  (113) Paraquat 5 mM 48 hrs, 4-day adult  (103) Paraquat 10 mM 48 hrs, 4-day adult  (80) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  (29) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (119) cold shock, 4-day adult    96 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    34 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    71 Cadmium 100 mM 48 hrs, 4-day adult  (118) Copper 0.5 mM 12 hrs, larvae L3    72 Copper 15 mM 48 hrs, 4-day adult  104 Zinc 5 mM 12 hrs, larvae L3    79 Zinc 4.5 mM 48 hrs, 4-day adult    70 Ethanol 2.5% 3 hrs, larvae L3  (110) Ethanol 5% 3 hrs, larvae L3    31 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  102 Caffeine 25 mg/ml 48 hrs, 4-day adult  (113) Paraquat 5 mM 48 hrs, 4-day adult  103 Paraquat 10 mM 48 hrs, 4-day adult    80 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    96 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    34 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    71 Cadmium 100 mM 48 hrs, 4-day adult    118 Copper 0.5 mM 12 hrs, larvae L3    72 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    79 Zinc 4.5 mM 48 hrs, 4-day adult    70 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    31 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    113 Paraquat 5 mM 48 hrs, 4-day adult    103 Paraquat 10 mM 48 hrs, 4-day adult    80 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    96 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    34 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    71 Cadmium 100 mM 48 hrs, 4-day adult    118 Copper 0.5 mM 12 hrs, larvae L3    72 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    79 Zinc 4.5 mM 48 hrs, 4-day adult    70 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    31 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    113 Paraquat 5 mM 48 hrs, 4-day adult    103 Paraquat 10 mM 48 hrs, 4-day adult    80 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (119) cold shock, 4-day adult  (96) heat shock, 4-day adult  (74) Cadmium 50 mM 6 hrs, larvae L3  (34) Cadmium 50 mM 12 hrs, larvae L3  (44) Cadmium 50 mM 48 hrs, 4-day adult  (71) Cadmium 100 mM 48 hrs, 4-day adult  (118) Copper 0.5 mM 12 hrs, larvae L3  (72) Copper 15 mM 48 hrs, 4-day adult  (104) Zinc 5 mM 12 hrs, larvae L3  (79) Zinc 4.5 mM 48 hrs, 4-day adult  (70) Ethanol 2.5% 3 hrs, larvae L3  (110) Ethanol 5% 3 hrs, larvae L3  31 Ethanol 10% 3 hrs, larvae L3  (35) Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (102) Caffeine 25 mg/ml 48 hrs, 4-day adult  (113) Paraquat 5 mM 48 hrs, 4-day adult  (103) Paraquat 10 mM 48 hrs, 4-day adult  (80) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  29 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  119 cold shock, 4-day adult    96 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    34 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    71 Cadmium 100 mM 48 hrs, 4-day adult  118 Copper 0.5 mM 12 hrs, larvae L3    72 Copper 15 mM 48 hrs, 4-day adult  104 Zinc 5 mM 12 hrs, larvae L3    79 Zinc 4.5 mM 48 hrs, 4-day adult    70 Ethanol 2.5% 3 hrs, larvae L3  110 Ethanol 5% 3 hrs, larvae L3    31 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  102 Caffeine 25 mg/ml 48 hrs, 4-day adult  113 Paraquat 5 mM 48 hrs, 4-day adult  103 Paraquat 10 mM 48 hrs, 4-day adult    80 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    96 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    34 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    71 Cadmium 100 mM 48 hrs, 4-day adult    118 Copper 0.5 mM 12 hrs, larvae L3    72 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    79 Zinc 4.5 mM 48 hrs, 4-day adult    70 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    31 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    113 Paraquat 5 mM 48 hrs, 4-day adult    103 Paraquat 10 mM 48 hrs, 4-day adult    80 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004237 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12749-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Hrb87FGD7818 viable Hrb87FCC00189 Hrb87FY1217 Other Phenotypes Allele neuroanatomy defective | larval stage Hrb87FGD7818 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage Hrb87FGD7818 ganglion mother cell | larval stage Hrb87FGD7818
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Hrb87F Class Mutagen Stocks Known lesion Hrb87FBG02743 1 -- Hrb87Fc02654 1 -- Hrb87FKG02089 P-element activity 1 -- Hrb87F28 Delta2-3 0 Yes Hrb87F5-HA-1584 0 -- Hrb87F7 Delta2-3 0 Yes Hrb87FCC00189 P-element activity 0 Yes Hrb87FY1217 wild-type allele P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hrb87F Class Mutagen Stocks Known lesion Hrb87FGD7818 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hrb87FJF01250 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hrb87FJF01757 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hrb87FKK108162 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hrb87Fhs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes Hrb87FNIG.12749R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Hrb87FScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes Hrb87FtHa in vitro construct - genomic fragment 0 Yes Hrb87FtHb in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)B5228 (Peng and Mount, 1995) Df(3R)B52R1 (Peng and Mount, 1995) Df(3R)Exel6169 (Ryder, 2004.4.26) Df(3R)Hrb87F (Zu et al., 1996, Sengupta and Lakhotia, 2006, Singh and Lakhotia, 2012, Mallik and Lakhotia, 2010) Df(3R)Hrb87F7 (Peng and Mount, 1995) Df(3R)l26c (Peng and Mount, 1995) Df(3R)urd (Peng and Mount, 1995)
Not disrupted in	Df(3R)red3l (Peng and Mount, 1995) Df(3R)ry27 (Peng and Mount, 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7818} NA P{KK108162} NA P{NIG.12749R} NA P{TRiP.JF01250} NA P{TRiP.JF01757} NA P{UAS-Hrb87F.S} NA heat-shock construct P{FLAG/HRB87F} NA characterization construct P{Hrb87F.Hb} NA P{Hrb87F} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}Hrb87FBG02743 NA P{PTT-GC}Hrb87FCC00189 NA P{RS5}Hrb87F5-HA-1584 NA P{SUPor-P}Hrb87FKG02089 NA PBac{PB}Hrb87Fc02654 NA insertion of enhancer trap P{lacW}Hrb87FY1217 No
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding inferred from direct assay (Borah et al., 2009)
Biological Process
CV term Evidence References
compound eye morphogenesis inferred from genetic interaction with human CELF1 (Sofola et al., 2007) mitosis inferred from mutant phenotype (Ducat et al., 2008) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) regulation of alternative mRNA splicing, via spliceosome inferred from mutant phenotype (Park et al., 2004, Borah et al., 2009)
Cellular Component
CV term Evidence References
chromatin inferred from direct assay (Prasanth et al., 2000) nucleoplasm inferred from direct assay (Matunis et al., 1992) nucleus inferred from direct assay (Prasanth et al., 2000) omega speckle inferred from direct assay (Prasanth et al., 2000) polytene chromosome puff inferred from direct assay (Buszczak and Spradling, 2006, Matunis et al., 1992) ribonucleoprotein complex inferred from direct assay (Matunis et al., 1992) inferred from physical interaction with sqd (Matunis et al., 1992)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
nucleus inferred by curator from GO:0000381 (Park et al., 2004) ribonucleoprotein complex inferred from sequence or structural similarity with human HNRPA1 (Matunis et al., 1992) non-traceable author statement (Hovemann, 1991.2.28, Swiss-Prot Project Members, 1996.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hrb87F - Hrb98DE experimental knowledge based (Guruharsha et al., 2011) Hrb87F - bl anti bait coimmunoprecipitation, western blot (Hovemann et al., 2000) Hrb87F - glo experimental knowledge based (Guruharsha et al., 2011) Hrb87F - nej anti bait coimmunoprecipitation, western blot (Mallik and Lakhotia, 2010)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hrb87F allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 71477 DroID - A comprehensive database of gene and protein interactions. • FBgn0004237 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48535
Orthologs
OrthoDB Orthologs (154) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6D53J0 Dipteran inclusive ortholog search EOG6W3SX3 Insect inclusive ortholog search EOG65TBJ9 Arthropod inclusive ortholog search EOG6X69RF Metazoa inclusive ortholog search EOG6G20CW
Orthologs in Drosophila Species (EOG6D53J0)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hrb87F     Drosophila sechellia   Dsec\GM25918 Y   Drosophila erecta   Dere\GG17033 Y   Drosophila yakuba   Dyak\GE24426 Y   Drosophila ananassae   Dana\GF17368 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA26465 Y   Drosophila willistoni   Dwil\GK13334 Y   Drosophila virilis   Dvir\GJ23111 Y   Drosophila mojavensis   Dmoj\GI10692 Y   Drosophila grimshawi   Dgri\GH19058 Y
Orthologs in non-Drosophila Dipterans (EOG6W3SX3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010467 Y Anopheles gambiae Malaria mosquito  Agam\AGAP002374 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001612 Y
Orthologs in non-Dipteran Insects (EOG65TBJ9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB41392 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG008807 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19277 Y Atta cephalotes Leafcutter ant  Acep\ACEP15101 Y Linepithema humile Argentine ant  Lhum\LH16171 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17027 Y Solenopsis invicta Red fire ant  Sinv\SINV24695 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009542 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001865 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002624 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI003661 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002060 Y Bombyx mori Silkmoth  Bmor\BGIBMGA007822 Y Bombyx mori Silkmoth  Bmor\BGIBMGA006405 Y Pediculus humanus Human body louse  Phum\PHUM050860 Y Pediculus humanus Human body louse  Phum\PHUM451750 Y Tribolium castaneum Red flour beetle  Tcas\TC004556 Y
Orthologs in non-Insect Arthropods (EOG6X69RF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_307331 Y Ixodes scapularis Deer tick  Isca\ISCW011438 Y
Orthologs in non-Arthropod Metazoa (EOG6G20CW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\sqd-1 Y Caenorhabditis elegans Nematode  Cele\hrp-1 Y Caenorhabditis elegans Nematode  Cele\H28G03.1 Y Caenorhabditis elegans Nematode  Cele\msi-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022512gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008172gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015676gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020274gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026906gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_002003gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007318gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017609gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024306gn Y Danio rerio Zebrafish  Drer\hnrnpd Y Danio rerio Zebrafish  Drer\hnrnpa1 Y Danio rerio Zebrafish  Drer\zgc:66127 Y Danio rerio Zebrafish  Drer\dazap1 Y Danio rerio Zebrafish  Drer\msi2a Y Danio rerio Zebrafish  Drer\hnrnpab Y Danio rerio Zebrafish  Drer\msi2b Y Danio rerio Zebrafish  Drer\hnrnpa0l Y Danio rerio Zebrafish  Drer\hnrpdl Y Danio rerio Zebrafish  Drer\zgc:77366 Y Danio rerio Zebrafish  Drer\msi1 Y Danio rerio Zebrafish  Drer\zgc:77052 Y Danio rerio Zebrafish  Drer\hnrnpa3 Y Danio rerio Zebrafish  Drer\hnrnpa0 Y Xenopus tropicalis Western clawed frog  Xtro\dazap1 Y Xenopus tropicalis Western clawed frog  Xtro\msi1 Y Xenopus tropicalis Western clawed frog  Xtro\hnrnpd Y Xenopus tropicalis Western clawed frog  Xtro\hnrnpa2b1 Y Xenopus tropicalis Western clawed frog  Xtro\hnrpdl Y Xenopus tropicalis Western clawed frog  Xtro\hnrnpa0 Y Xenopus tropicalis Western clawed frog  Xtro\hnrnpa1 Y Xenopus tropicalis Western clawed frog  Xtro\hnrnpa3 Y Xenopus tropicalis Western clawed frog  Xtro\hnrnpab Y Gallus gallus Domestic chicken  Ggal\DAZAP1 Y Gallus gallus Domestic chicken  Ggal\HNRNPA0 Y Gallus gallus Domestic chicken  Ggal\HNRNPA2B1 Y Gallus gallus Domestic chicken  Ggal\HNRNPAB Y Gallus gallus Domestic chicken  Ggal\HNRNPA3 Y Gallus gallus Domestic chicken  Ggal\HNRPDL Y Gallus gallus Domestic chicken  Ggal\MSI2 Y Gallus gallus Domestic chicken  Ggal\HNRNPD Y Gallus gallus Domestic chicken  Ggal\MSI2 Y Mus musculus House mouse  Mmus\Gm6793 Y Mus musculus House mouse  Mmus\Dazap1 Y Mus musculus House mouse  Mmus\Hnrnpa3 Y Mus musculus House mouse  Mmus\Hnrpdl Y Mus musculus House mouse  Mmus\Hnrnpa1 Y Mus musculus House mouse  Mmus\Gm7551 Y Mus musculus House mouse  Mmus\Gm8991 Y Mus musculus House mouse  Mmus\Msi1 Y Mus musculus House mouse  Mmus\ENSMUSG00000004980 Y Mus musculus House mouse  Mmus\Hnrnpab Y Mus musculus House mouse  Mmus\Rbm31y Y Mus musculus House mouse  Mmus\Hnrnpa0 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031608 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021254 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000013650 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000030250 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032168 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000023438 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031972 Y Rattus norvegicus Norway rat  Rnor\Msi1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031316 Y Rattus norvegicus Norway rat  Rnor\Hnrpdl Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021280 Y Rattus norvegicus Norway rat  Rnor\RGD1564416 Y Rattus norvegicus Norway rat  Rnor\Msi2 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000025891 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024652 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042093 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021929 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032584 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000017651 Y Rattus norvegicus Norway rat  Rnor\Dazap1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029641 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000039436 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000005951 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000007252 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000027915 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021986 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024895 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042377 Y Rattus norvegicus Norway rat  Rnor\Hnrnpa2b1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000011100 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000005671 Y Rattus norvegicus Norway rat  Rnor\Hnrnpa1 Y Rattus norvegicus Norway rat  Rnor\Hnrpd Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000017364 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029434 Y Rattus norvegicus Norway rat  Rnor\Hnrnpab Y Rattus norvegicus Norway rat  Rnor\LOC100360986 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000039876 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029531 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033211 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042924 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000043173 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000039983 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038905 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033182 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000027605 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022655 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000025482 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029898 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000030901 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032616 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000027006 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038392 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022285 Y Homo sapiens Human  Hsap\HNRNPA0 Y Homo sapiens Human  Hsap\DAZAP1 Y Homo sapiens Human  Hsap\HNRNPA2B1 Y Homo sapiens Human  Hsap\ENSG00000197451 Y Homo sapiens Human  Hsap\HNRNPD Y Homo sapiens Human  Hsap\MSI2 Y Homo sapiens Human  Hsap\HNRNPA1L2 Y Homo sapiens Human  Hsap\ENSG00000135486 Y Homo sapiens Human  Hsap\HNRNPA3 Y Homo sapiens Human  Hsap\HNRPDL Y Homo sapiens Human  Hsap\MSI1 Y
Human Orthologs (11)
Gene OMIM HGNC Hsap\HNRNPA0     Hsap\DAZAP1     Hsap\HNRNPA2B1     Hsap\ENSG00000197451     Hsap\HNRNPD     Hsap\MSI2     Hsap\HNRNPA1L2     Hsap\ENSG00000135486     Hsap\HNRNPA3     Hsap\HNRPDL     Hsap\MSI1
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM25918 Drosophila erecta Dere\GG17033 Drosophila yakuba Dyak\GE24426 Drosophila ananassae Dana\GF17368 Drosophila pseudoobscura pseudoobscura Dpse\GA26465 Drosophila willistoni Dwil\GK13334 Drosophila virilis Dvir\GJ23111 Drosophila mojavensis Dmoj\GI10692 Drosophila grimshawi Dgri\GH19058
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	12869 w1118; P{GT1}Hrb87FBG02743 31244 y1 v1; P{TRiP.JF01757}attP2 31472 y1 v1; P{TRiP.JF01250}attP2/TM3, Ser1 14414 y1 w67c23; ry506 P{SUPor-P}Hrb87FKG02089
Harvard	- PBac{PB}Hrb87Fc02654
VDRC	v100732 P{KK108162}VIE-260B v51759 w1118; P{GD7818}v51759
Genomic Clones ( 1 )
	BACR03J04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 135 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH05625 LD32727
Other clones	MIP15350
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT06910 AT19510 AT30712 GH14932 GH22933 GH26386 GM03560 GM06625 GM19619 GM20443 GM26859 LD01025 LD03134 LD03807 LD04368 LD05381 LD07995 LD19802 LD23806 LD26395 LD27730 LD28121 LD28568 LD29935 LD30259 LD31565 LD32751 LD36848 LD37811 LD38986 LD40958 LD41654 LD42313 LD44335 LP11627 LP11908 LP21807 LP22257 LP22532 RE05213 RE06038 RE12271 RE13503 RE16046 RE16489 RE17928 RE22710 RE25316 RE26430 RE47773 RE48739 RE50639 RE61773 RE62218 RE64349 RE65741 RE67422 RE72738 RE73182 RE74556 SD18727 SD20056 SD22114 SD24333 SD26901
Other clones	13667 28163 30120 34402 36467 52732 54523 70074 70947 72118 75160 76758 82891 99744 101751 104057 119983 174568 179713 191317 193054 276952 bs79e07 CK01.1B.D1 CK01.26A.F4 CK01.92D.E3 CK01.168A.F7 Dmel_dig_010243_0647_0743 Dmel_dig_018035_0680_1087 Dmel_neb_014001_0842_3574 Dmel_neb_015954_0581_1215 EC30471 EK024841 EK104516 EK115512 EK147553 EK148755 EK168519 EK180732 EK196903 EK200056 EK200411 EK206057 EK216748 EK222332 EK227551 EK251554 EK279324 EK294034 EK295922 EK303644 RP004041757 RP004119385 RP004203445 RP004228141 RP004230455 RP004240306 RP004242180 RP004243362 RP004253693 RP004253860 RP004258353 RP004270140 RP004279341 RP004440203 RP004522328 RP004526419
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004237 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48535
Antibody Information
	monoclonal (Matunis et al., 1992) polyclonal (Hovemann et al., 1991, Blanchette et al., 2009, Borah et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Hrb87F CG12749 (Bayraktaroglu, 2000.4.12) Source for merge of: Hrb87F Hrb85CD (Mount, 2000.10.26)
Additional comments
Other Comments
	Absence of Hrb87F affects starvation and thermal stress tolerance. (Singh and Lakhotia, 2012) Loss of Hrb87F slows development, with a significant reduction in adult life span, decreased female fecundity and high sensitivity to starvation and thermal stresses. In the absence of Hrb87F, the nucleoplasmic omega speckles are nearly completely disrupted. (Singh and Lakhotia, 2012) The consequences of HRB overexpression and absence in living flies is examined. Overexpression of Hrb87F does alter the splicing of Ddc pre-mRNA, indicating the overexpressed protein can reproduce the in vitro effect of excess protein. (Zu et al., 1996) Complete deletion of Hrb87F has no obvious defect on viability or fertility, but when in combined with hypodosage of Hrb98DE leads to reduced male and female fertility. (Haynes et al., 1995) The distribution of the hnRNP protein Hrb87F on nascent transcripts from polytene chromosomes has been studied. (Matunis et al., 1993) An Hrb87F cDNA clone has been isolated and sequenced. (Matunis et al., 1992) Isolated from an early embryonic cDNA library under reduced stringency conditions, using an Hrb98DE cDNA probe. Some Canton S strains have a 412 element insertion in the 3' untranslated region. (Haynes et al., 1991) Hrb87F has been cloned and sequenced. (Haynes et al., 1991, Hovemann et al., 1991) 412 element inserted in 3' UTR in some Canton S lines   Homology to the mammalian A and B hnRNP proteins based on amino acid sequence inferred from nucleotide sequence.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057458 NM_057459 NM_001170131 NM_001275609 RefSeq (Proteins) • NP_476806 NP_476807 NP_001163602 NP_001262538 DDBJ / EMBL / GenBank • AA978720 AAF54967 AAN13574 AC007646 ACZ94899 AGB95920 AI107775 AI945591 BH854519 BH854520 CAA38574 CAA41170 CAA42212 CAA44502 X54803 X58183 X58184 X59691 X62636 Entrez Gene - A searchable database of RefSeq genes. • 48535 UniProtKB/Swiss-Prot • P48810 UniProtKB/TrEMBL • E1JIK0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677)
Linkouts
	BioGRID - A database of protein and genetic interactions • 71477 DroID - A comprehensive database of gene and protein interactions. • FBgn0004237 DRSC - Results from RNAi screens. • FBgn0004237 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004237 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12749-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004237 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48535 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48535 modMine - Data generated by the modENCODE project. • FBgn0004237
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	CG12749 (Brody et al., 2002, Ducat et al., 2008, Andersen and Tapon, 2008, Pisa et al., 2009, Keleman et al., 2009.8.5, McIntyre et al., 2006, Ni et al., 2008, Cammarato et al., 2011, Olson et al., 2007) hnRNP36 (Ni et al., 2006) hnRNP A2/B1 (Tan et al., 2012) Hrb2   Hrb85CD   Hrb87f (Clouse and Schupbach, 2003, Sofola et al., 2007, Onorati et al., 2011) Hrb87F (Sengupta and Lakhotia, 2006, Rasmussen and Kellis, 2007, Sofola et al., 2007, Buszczak et al., 2007, Manita et al., 2011, Hurt et al., 2009, Pisa et al., 2009, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2009, Piacentini et al., 2009, McIntyre et al., 2006, Baig et al., 2010, Mallik and Lakhotia, 2010, Shukla and Tapadia, 2011, Neumüller et al., 2011, Olson et al., 2007, Tan et al., 2012, Japanese National Institute of Genetics, 2012.5.21) HRB87F (Sofola et al., 2007, Akanksha et al., 2008) HRB87F/hrp36 (Beyer et al., 1996) Hrb87Fa (Burnette et al., 1999, ) hrp36 (Park et al., 2004, Weeks et al., 1993, Matunis et al., 1993, Matunis et al., 1992, Matunis et al., 1992, Sofola et al., 2007, Graveley et al., 2008, Blanchette et al., 2009, Borah et al., 2009, Olson et al., 2007) HRP36 (Turnage et al., 2000) Hrp36 (Lakhotia et al., 1999, Hessle et al., 2009, Verma et al., 2012, Singh and Lakhotia, 2012) hrp40 (Reim et al., 1999) P11 (Hovemann et al., 2000, Lakhotia et al., 1999, Hovemann, 1991.2.28, Hovemann, 1991.2.28, Hovemann et al., 1991, Buszczak and Spradling, 2006) P11/Hrb87F (Reim et al., 1999) p38 (Reim et al., 1999) Q14 (Lakhotia et al., 1999) Q16 (Lakhotia et al., 1999)
Name Synonym	Heterogeneous nuclear ribonucleoprotein at 85CD   Heterogeneous nuclear ribonucleoprotein at 87F (Pisa et al., 2009, Baig et al., 2010, Cammarato et al., 2011) hnRNA-binding protein 2
Secondary FlyBase IDs
	FBgn0010230
References ( 99 )
Generate a list of	All references relating to this gene ( 99 )
List References by type	Research paper  ( 61 ) Supplementary material  ( 6 ) Review  ( 3 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 10 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 10 )
	Crawford et al., 2012, Cell Death Differ. 19(12): 2040--2048 Conservation of caspase substrates across metazoans suggests hierarchical importance of signaling pathways over specific targets and cleavage site motifs in apoptosis. [FBrf0220233] Dwivedi et al., 2012, PLoS ONE 7(5): e37113 In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications. [FBrf0218341] Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134 Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342] Singh and Lakhotia, 2012, J. Biosci., Bangalore 37(4): 659--678 The hnRNP A1 homolog Hrp36 is essential for normal development, female fecundity, omega speckle formation and stress tolerance in Drosophila melanogaster. [FBrf0219285] Tan et al., 2012, Hum. Mol. Genet. 21(1): 57--65 Retrotransposon activation contributes to fragile X premutation rCGG-mediated neurodegeneration. [FBrf0216915] Verma et al., 2012, genesis 50(2): 86--101 DNApol‐ϵ gene is indispensable for the survival and growth of Drosophila melanogaster. [FBrf0217413] Manita et al., 2011, Bioorg. Med. Chem. 19(24): 7690--7697 Camptothecin (CPT) directly binds to human heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and inhibits the hnRNP A1/topoisomerase I interaction. [FBrf0216744] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Onorati et al., 2011, PLoS Genet. 7(5): e1002096 The ISWI Chromatin Remodeler Organizes the hsrω ncRNA–Containing Omega Speckle Nuclear Compartments. [FBrf0213856] Shukla and Tapadia, 2011, Europ. J. Cell Biol. 90(1): 72--80 Differential localization and processing of apoptotic proteins in Malpighian tubules of Drosophila during metamorphosis. [FBrf0212365] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...