Diptericin, Dpt, Dipt, Dip, DiptA
Gene model reviewed during 5.50
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DptA using the Feature Mapper tool.
Dpt transcripts are expressed in response to inoculation with a bacterial suspension. They are detected in adult males 2 hours after inoculation and reach a peak at 4 hours after inoculation. A similar response was observed in larvae and late pupae. A low level of Dpt transcripts was observed in untreated pupae and adults.
GBrowse - Visual display of RNA-Seq signalsView Dmel\DptA in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: DptA Dpt
Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. Shows alteration in expression in a Clk mutant background.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Ecol\lacZDpt.PR adults are pricked with a sterile needle dipped in culture pellets of various living microorganisms (distinct bacterial strains, fungal spores or hyphae). Pricking results in a low but clearly detectable expression of all antimicrobial genes and these genes are induced above the background level by specific classes of microorganism.
Antibacterial genes are not induced in L.boulardi resistant strains of Drosophila suggesting they cannot be involved directly in the antiparasitoid response.
The κB motifs of Dpt and CecA1 are not functionally equivalent. Mutants carrying no copies of dl and a single copy of Dif retain their full capacity to express the Dpt and CecA1 genes in response to bacterial challenge.
Genes encoding antibacterial peptides are regulated in a manner distinct from that of Drs, encoding the antifungal peptide.
The increase, or maturation, of Dpt promoter activity during the third larval instar in response to bacterial challenge is ecdysteroid- dependent, as demonstrated using wild type and ecd1 or dor22 mutant larvae.
Transcription is not strongly induced by hymenopteran parasitoids.
A 45kD protein that binds sequence-specifically to the Dpt promoter GAAANN motif in fat body and blood cell extracts has been identified.
Ecol\lacZ reporter gene constructs demonstrate that a 2.2kb upstream sequence can only confer full bacterial inducibility when it carries both 17bp motifs in the proximal promoter region.
Analysis of the Dpt promoter defined two 17bp repeats essential for LPS inducibility. Addition of extra copies of the 17bp repeat increases the level of transcription on LPS induction. A specific DNA-protein binding activity present only in induced blood cells and fat body suggests an induction mechanism similar to that of mammalian NF-κB.
Dpt shows at least 4 distinct expression phases: young larvae, late third instar larvae, pupae and adults. The complexity may be related to the presence of multiple copies of response elements in the Dpt upstream sequences.
Isolated from a cDNA library using probes complementary to residues 38-43 of Phormia diptericin A.