General Information
Symbol
Dmel\Rdl
Species
D. melanogaster
Name
Resistant to dieldrin
Annotation Symbol
CG10537
Feature Type
FlyBase ID
FBgn0004244
Gene Model Status
Stock Availability
Gene Snapshot
Resistant to dieldrin is a widely-expressed ligand-gated chloride channel which mediates the fast inhibitory effects of GABA. It has been shown to have roles in the circuits underlying visual processing, odor coding, learning and memory, sleep and courtship behavior. [Date last reviewed: 2016-06-30]
Also Known As
GABAAR, GABAA-R, DmRdl, LCCH1, GABAA receptor
Genomic Location
Cytogenetic map
Sequence location
3L:9,145,838..9,182,149 [-]
Recombination map
3-28
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. (P25123)
Molecular Function (see GO section for details)
Summaries
Gene Group Membership
GABA-GATED CHLORIDE CHANNEL SUBUNITS -
GABA-gated chloride channels mediate synaptic inhibition and regulate cellular excitability. GABA is the principal inhibitory neurotransmitter in the insect nervous system. Upon GABA binding the pore will open to allow chloride anions to pass, leading to hyperpolarization. (Adapted from FBrf0232136 and FBrf0228637).
UniProt Contributed Function Data
GABA, an inhibitory neurotransmitter, mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel.
(UniProt, P25123)
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
5

Please see the GBrowse view of Dmel\Rdl or the JBrowse view of Dmel\Rdl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.40
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0076534
3683
606
FBtr0076536
8583
606
FBtr0309054
3821
579
FBtr0333834
8583
606
FBtr0333835
8583
606
FBtr0333836
8589
608
Additional Transcript Data and Comments
Reported size (kB)
2.1 (longest cDNA)
2.066 (longest cDNA)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0076261
65.7
606
9.46
FBpp0076263
65.8
606
9.27
FBpp0301075
62.9
579
9.45
FBpp0305968
65.6
606
9.37
FBpp0305969
65.7
606
9.46
FBpp0305970
66.0
608
9.46
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

606 aa isoforms: Rdl-PA, Rdl-PF
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
This GABA subunit functions efficiently as a homooligomeric complex in Xenopus oocytes. The receptors formed by this subunit exhibit unique pharmacological properties when compared with vertebrate A and ρ receptors.
one of 3 alternative start sites on one transcript
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rdl using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (27 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR002289
(assigned by InterPro )
traceable author statement
Biological Process (14 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
Cellular Component (9 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
non-traceable author statement
(assigned by UniProt )
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000434994
(assigned by GO_Central )
Expression Data
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Rdl protein is expressed in the neuronal processes that overlap the insulin producing cells (IPC), a subset of the dorsomedial neurosecretory cells of the pars intercerebralis.
Rdl is expressed strongly in the soma of l-LNv neurons and at much lower levels in s-LNv neurons. Rdl puncta are also seen close to LNv terminals of the accessory medulla and in PDF varicosities formed by l-LNv neurites in the optic lobe.
Rdl protein is expressed in the small and large Pdf-positive LNv neurons and possibly in the fifth Pdf-negative s-LNv.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rdl in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 22 )
Transgenic Constructs ( 28 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rdl
Allele of Rdl
Mutagen
Associated Transgenic Construct
Stocks
Transgenic constructs containing regulatory region of Rdl
GAL4 construct
Name
Expression Data
reporter construct
Deletions and Duplications ( 14 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (25)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
4 of 15
Yes
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
 
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
 
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
 
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (24)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
4 of 15
Yes
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (22)
4 of 13
Yes
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (11)
3 of 12
Yes
No
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (32)
4 of 15
Yes
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (23)
13 of 15
Yes
Yes
6 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (20)
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190791 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915047D )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0553 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X047C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0805 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 1 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please look at the Interaction Group reports for full details of the physical interactions
    protein-protein
    Interacting group
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-28
    Cytogenetic map
    Sequence location
    3L:9,145,838..9,182,149 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    67A1-67A1
    Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    66F-66F
    (determined by in situ hybridisation)
    66F1-67A1
    (determined by in situ hybridisation)
    66F-67A2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (27)
    Genomic Clones (33)
    cDNA Clones (42)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Additional comments
      Other Comments
      4kb transcript identified during molecular analysis of the Rdl genomic region.
      The actions of a volatile anaesthetic on a GABA-activated chloride channel (Rdl) and the implications of a channel directed point mutation (conferring insensitivity to PTX) on the modulation of the inhibitory currents by isoflurane are studied.
      Although Rdl and Lcch3 can coassemble in heterologous expression systems they may not be found in the same tissues in the nervous system.
      Pharmacological characterisation of Rdl.
      The distribution of Rdl-like subunits in the nervous system is investigated using an antibody raised against the region of Rdl.
      In situ hybridisation reveals the Rdl message and protein are highly expressed in the developing central nervous system, Rdl GABA subunits are confined to the CNS.
      Polyclonal antibody staining shows Rdl protein to be confined to the CNS in adult flies.
      Experiments with picrotoxin and four of its analogues suggest that interactions of convulsants with homo-oligomeric and multimeric GABA receptors are qualitatively similar. The Rdl homo-oligomer exhibits a pharmacological profile resembling that of native GABA receptors.
      Subunit composition determines picrotoxin and bicuculline sensitivity of GABA receptors.
      The Rdl product GABA receptor exhibits a unique pharmacological profile and functions efficiently as a homo-oligomer.
      Rdl encodes a GABA-activated chloride channel with novel pharmacology in comparison to mammalian GABA receptors, and functions efficiently as a homomer.
      When Rdl cRNA is expressed in Xenopus oocytes the product forms a functional GABA-receptor homo-oligomer.
      Rdl is stably expressed in a culture cell line and pharmacological and electrophysiological aspects of the encoded receptor are characterised.
      Rdl mutations manifest cyclodiene resistance through two different mechanisms. The A302S mutation weakens drug binding to the antagonist-favoured (desensitized) conformation by a structural change at the drug binding site. It also destabilizes the antagonist-favoured conformation in an allosteric sense. The global association of resistance with the A302S mutation suggests that insecticides have selected residue 302 for replacement because of its unique ability to influence both of these functions. The location of alanine 302 in the sequence of Rdl supports a mechanism of action in which convulsants such as picrotoxin bind within the channel lumen, and induce a conformational change to the desensitized state.
      Cloning and characterisation of the complete coding region of a cDNA corresponding to Rdl.
      The functional expression of both cyclodiene susceptible and resistant Rdl homomultimers of two alternative splice forms is studied in a baculovirus insect cell system.
      Rdl shows extensive alternative splicing.
      Rdl confers insensitivity to cyclodiene insecticides. Sequence analysis of the insensitive allele has allowed the molecular basis of channel insensitivity to be identified.
      The same mutation was found in many resistant populations in D.melanogaster. An identical mutation, or another substitution in the same amino acid position in the D.simulans gene has been identified in resistant populations of D.simulans.
      The genomic organisation of the Rdl locus has been determined.
      Susceptibility to cyclodiene is related to the ratio of susceptible and resistant alleles, not to the absolute number of either.
      The Rdl GABA receptor has been cloned via a chromosomal walk and allowed manipulation of the resistance status of an individual to cyclodiene via germ-line transformation.
      Inhibitory Rdl and Grd gene products are encoded by a superfamily of related genes.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 65 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Linkouts
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Synonyms and Secondary IDs (26)
      Reported As
      Symbol Synonym
      GABAr
      Rdl
      (Perry and Batterham, 2018, Zhang et al., 2018, Cheung and Scott, 2017, Choi et al., 2017, Fisher et al., 2017, Franco et al., 2017, Li et al., 2017, Seugnet et al., 2017, Suh et al., 2017, Tomita et al., 2017, Transgenic RNAi Project members, 2017-, Crocker et al., 2016, Ffrench-Constant et al., 2016, Graham et al., 2016, Hong et al., 2016, Pavlou et al., 2016, Aradska et al., 2015, Diao et al., 2015, Dissel et al., 2015, Donelson and Sanyal, 2015, Fisher et al., 2015, Gene Disruption Project members, 2015-, Haynes et al., 2015, Kern et al., 2015, Omelyanchuk et al., 2015, Petruccelli et al., 2015, Ashwal-Fluss et al., 2014, Li et al., 2014, Liu et al., 2014, Liu et al., 2014, Remnant et al., 2014, Singh et al., 2014, Ganguly and Lee, 2013, Ghezzi et al., 2013, Kuehn and Duch, 2013, Lei et al., 2013, Potdar and Sheeba, 2013, Remnant et al., 2013, Stocker et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Paranjpe et al., 2012, Ren et al., 2012, Rodriguez et al., 2012, Ruben et al., 2012, Sudhakaran et al., 2012, Tsubouchi et al., 2012, Keegan et al., 2011, Rolls, 2011, Stilwell, 2011.4.18, Hekmat-Scafe et al., 2010, Knipple and Soderlund, 2010, Busch et al., 2009, Chung et al., 2009, Jones et al., 2009, Liu et al., 2009, Okada et al., 2009, Seugnet et al., 2009, Agosto et al., 2008, Hoopfer et al., 2008, Parisky et al., 2008, Yang et al., 2008, Christensen et al., 2007.10.29, Liu et al., 2007, Narusuye et al., 2007, Raghu et al., 2007, D'Hulst et al., 2006, Gresens et al., 2006.7.11, Hekmat-Scafe et al., 2006, Hosie et al., 2006, Iovchev et al., 2006, Stilwell et al., 2006, Sanchez-Soriano and Prokop, 2005, Witte et al., 2002, Hosie et al., 1995)
      Secondary FlyBase IDs
      • FBgn0010390
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (283)