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Gene Dmel\18w

General Information
Symbol	Dmel\18w	Species	D. melanogaster
Name	18 wheeler	Annotation symbol	CG8896
Feature type	protein_coding_gene	FlyBase ID	FBgn0004364
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	tlr
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	56F8-56F8	Sequence location	2R:15,999,016..16,004,437 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene 18 wheeler is referred to in FlyBase by the symbol Dmel\18w (CG8896, FBgn0004364). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: transmembrane signaling receptor activity. There is experimental evidence that it is involved in the biological process: immune response; cell adhesion; anatomical structure morphogenesis; antibacterial humoral response. 22 alleles are reported. The phenotypes of these alleles are annotated with: leg; eye; wing disc; antenna; adult abdomen; haltere; mesothoracic tergum; wing; ventral thoracic disc. It has one annotated transcript and one annotated polypeptide. Protein features are: Cysteine-rich flanking region, C-terminal; Leucine rich repeat 5; Leucine-rich repeat; Leucine-rich repeat, typical subtype; Leucine-rich repeat-containing N-terminal; Toll/interleukin-1 receptor homology (TIR) domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval trachea, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult hindgut, adult heart, adult fat body, adult salivary gland, adult ovary, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1626509_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of 18w. Gene sequence location is 2R:15999016..16004437.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 56F8-56F8   Limits computationally determined from genome sequence between P{lacW}l(2)k08002k08002 and P{lacW}Ate1k10809
Experimentally Determined Cytological Location
Cytogenetic map Notes References 56F6-56F9   (determined by in situ hybridisation)   (Spradling et al., 1999) 56F8-56F12   (determined by in situ hybridisation)   (Eldon and Bellen, 1992.4.17) 56F6-56F9     (BDGP Project Members, 1994-1999) 56F6-56F9   (determined by in situ hybridisation)   (Eldon and Bellen, 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\18w for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) 18w-RA FBtr0086309  NM_057466   5422   1385
Additional Transcript Data & Comments
Reported size (kB)	5.6 (northern blot) (Eldon et al., 1994, Chiang and Beachy, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein 18w-PA   FBpp0085620   154.8   1385   6.43     NP_476814   AAF57509
Additional Polypeptide Data & Comments
Reported size (kDa)	1389 (aa); 155 (kD predicted) (Eldon et al., 1994) 1385 (aa) (Chiang and Beachy, 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Toll/interleukin-1 receptor homology (TIR) domain (IPR000157) Cysteine-rich flanking region, C-terminal (IPR000483) Leucine-rich repeat (IPR001611) Leucine-rich repeat, typical subtype (IPR003591) Leucine-rich repeat-containing N-terminal (IPR000372) Leucine rich repeat 5 (IPR026906)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA802497   AE013599 AAF57509   AC004428   AC005286   AI403917   AQ025714   AY051592 AAK93016   BT125653   CO177657   CO276202   CO276905   CO280745   CO284814   CO326089   CO328338   DQ138938 ABA86544   EC084810   EC209091   EC220361   EC221965   EC222769   EC224860   EC254204   EC257922   L23171 AAA79208   S76155 AAB33383
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A1ZBR2 Q2XXS4 Q24591 Q7JPR9 Q961H0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435997 2R:15,990,593..15,991,303 evidence=Reporter construct (in vivo) (Junion et al., 2012, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage 8   amnioproctodeal invagination   (Eldon et al., 1994) embryonic stage 11   salivary gland body primordium   (Eldon et al., 1994) embryonic stage 12   labral segment   (Eldon et al., 1994) embryonic/larval posterior spiracle   (Eldon et al., 1994) embryonic stage 12 -- 17   tracheal system   (Eldon et al., 1994) embryonic stage 15 -- 16   anal pad   (Eldon et al., 1994) embryonic/larval pharynx | dorsal   (Eldon et al., 1994) adult midgut   (Eldon et al., 1994) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi central brain anlage in statu nascendi   (Fisher et al., 2012) dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi embryonic stage 5   organism | even pair rule expression pattern   (Chiang and Beachy, 1994) embryonic stage 6 -- 10   organism | segment polarity expression pattern   (Eldon et al., 1994) embryonic stage 7 -- 8   dorsal head epidermis anlage   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 8   cephalic furrow   (Eldon et al., 1994) embryonic stage 9 -- 10   dorsal ectoderm   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) Comment:reported as dorsal epidermis anlage dorsal head epidermis primordium   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 11   ventral nerve cord primordium   (Chiang and Beachy, 1994) tracheal pit   (Eldon et al., 1994) ventral midline   (Eldon et al., 1994) embryonic stage 11 -- 12   clypeo-labral primordium   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 12   anal plate   (Eldon et al., 1994) embryonic stage 12 -- 17   embryonic/larval dorsal vessel   (Eldon et al., 1994) embryonic/larval salivary gland   (Eldon et al., 1994) embryonic stage 13 -- 16   atrium   (Fisher et al., 2012) embryonic anal pad   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) labral segment   (Fisher et al., 2012) embryonic stage 15   embryonic/larval hindgut   (Eldon et al., 1994) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Chiang and Beachy, 1994, Tauszig et al., 2000) embryonic stage 7 -- 11   organism | segment polarity expression pattern   (Chiang and Beachy, 1994) embryonic stage 12 -- 17   labral segment   (Chiang and Beachy, 1994) embryonic/larval anterior spiracle   (Chiang and Beachy, 1994) embryonic/larval posterior spiracle   (Chiang and Beachy, 1994) tracheal pit   (Chiang and Beachy, 1994) embryonic stage 14 -- 17   haltere disc | presumptive   (Chiang and Beachy, 1994) anal plate   (Chiang and Beachy, 1994) wing disc | presumptive   (Chiang and Beachy, 1994) embryonic stage 16   maxillary palp sense organ   (Chiang and Beachy, 1994) embryonic/larval dorsal vessel   (Chiang and Beachy, 1994) antennal sense organ   (Chiang and Beachy, 1994)
Additional Descriptive Data
	18w transcripts are expressed in all stages of development tested. They are expressed in a complex pattern during embryogenesis. Expression is first observed just prior to cellular blastoderm formation in three stripes in the anterior portion of the embryo and a broader posterior domain. Subsequently, stripes are seen in the central region of the embryo. At late cellular blastoderm, secondary stripes appear leading to the transient expression of 18 stripes during early gastrulation and 20 stripes at stage 10. 18w expression overlaps the wg and en stripes and spans the parasegmental boundary. At maximum germ band extension, expression is observed along the ventral midline in small clusters of cells posterior to each stripe. Staining is also observed in salivary gland placodes, in tracheal pits, and in five areas in the presumptive head region. During germ band retraction, transcripts are less abundant and more restricted. They are observed in the developing tracheal system, the salivary gland anlagen, the anlagen for the anal plate, the posterior spiracles, the clypeolabrum, and in cells that participate in the formation of the dorsal vessel. In stage 15 and 16, 18w expression is also observed in regions of the head, pharynx, stomach, and hindgut. (Eldon et al., 1994) 18w is expressed in all developmental stages tested. Expression is first observed at the syncytial blastoderm stage in the presumptive hindgut, head regions, and parasegment 2. Shortly after, stripes of expression are seen first in the even numbered parasegments and then in the odd numbered parasegments. By the extended germ band stage, 18w is expressed in 15 transverse stripes with patches of expression corresponding to the 4 head segments and the hindgut as well. 18w expression in the trunk region was shown to overlap the wg and en stripes and spans the parasegmental boundary. At full germ band extension, the stripes begin to fade and expression is observed in segmentally repeated lateral clusters corresponding to the tracheal pits and the segmental grooves. Expression is accentuated in the cells surrounding the anterior and posterior spiracles. Transient expression in the CNS is also seen. Later, 18w expression is observed in the wing and haltere disc primordia, the dorsal vessel, the antennal-maxillary sense organs, the clypeolabrum, the anterior and posterior spiracles, and in the anal plate. (Chiang and Beachy, 1994) 18w transcripts are detected at all stages of development. (Tauszig et al., 2000)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Williams et al., 1997) plasma membrane inferred from direct assay (Williams et al., 1997)
Expression Deduced from Reporters
Reporter: P{A92}Q26b Stage Tissue/Position (including subcellular localization) Reference embryonic stage 6 -- 10   organism | segment polarity expression pattern   (Eldon et al., 1994) embryonic stage 11   salivary gland body primordium   (Eldon et al., 1994) tracheal pit   (Eldon et al., 1994) embryonic stage 14 -- 16   central nervous system | restricted   (Eldon et al., 1994) anal pad   (Eldon et al., 1994) embryonic/larval tracheal system   (Eldon et al., 1994) embryonic/larval dorsal vessel   (Eldon et al., 1994) embryonic/larval salivary gland   (Eldon et al., 1994) embryonic/larval hindgut   (Eldon et al., 1994) embryonic/larval pharynx   (Eldon et al., 1994) embryonic/larval posterior spiracle   (Eldon et al., 1994) embryonic/larval muscle system | ventral   (Eldon et al., 1994) adult stageoogenesis stage S3 -- S6   interfollicle cell   (Fasano and Kerridge, 1988) adult stageoogenesis stage S2 -- S6   basal stalk   (Fasano and Kerridge, 1988) adult stageoogenesis stage S8 -- S9   oocyte associated follicle cell   (Fasano and Kerridge, 1988) adult stage | female & oogenesis stage S14   egg operculum   (Fasano and Kerridge, 1988) adult stage & oogenesis stage S14   dorsal appendage   (Fasano and Kerridge, 1988) Reporter: P{lacW}A1-2-54 Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   organism | even pair rule expression pattern   (Chiang and Beachy, 1994) embryonic stage 7 -- 11   organism | segment polarity expression pattern   (Chiang and Beachy, 1994) embryonic stage 10 -- 17   anterior spiracle primordium | presumptive   (Hartenstein and Jan, 1992) epidermis & segment | posterior   (Hartenstein and Jan, 1992) embryonic brain | presumptive   (Hartenstein and Jan, 1992) salivary gland body primordium   (Hartenstein and Jan, 1992) posterior spiracle primordium   (Hartenstein and Jan, 1992) embryonic/larval tracheal system | restricted   (Hartenstein and Jan, 1992) ventral nerve cord | presumptive   (Hartenstein and Jan, 1992) epidermis | segmentally repeated   (Hartenstein and Jan, 1992) embryonic stage 11   ventral neurectoderm   (Chiang and Beachy, 1994) embryonic stage 12 -- 17   embryonic/larval posterior spiracle   (Chiang and Beachy, 1994) labral segment   (Chiang and Beachy, 1994) tracheal pit   (Chiang and Beachy, 1994) embryonic/larval anterior spiracle   (Chiang and Beachy, 1994) embryonic stage 14 -- 17   haltere disc | presumptive   (Chiang and Beachy, 1994) anal plate   (Chiang and Beachy, 1994) wing disc | presumptive   (Chiang and Beachy, 1994) embryonic stage 16   antennal sense organ   (Chiang and Beachy, 1994) maxillary palp sense organ   (Chiang and Beachy, 1994) embryonic/larval dorsal vessel   (Chiang and Beachy, 1994) third instar larval stage   wing disc   (Chiang and Beachy, 1994) haltere disc   (Chiang and Beachy, 1994) ventral thoracic disc   (Chiang and Beachy, 1994) eye-antennal disc   (Chiang and Beachy, 1994) optic lobe   (Chiang and Beachy, 1994) Reporter: P{PZ}18w00053 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | segmentally repeated   (Jackle and Janning, 1998.8.10) embryonic protocerebrum | restricted   (Jackle and Janning, 1998.8.10) anal pad   (Jackle and Janning, 1998.8.10) stomodeum   (Jackle and Janning, 1998.8.10) presumptive embryonic/larval central nervous system | restricted   (Jackle and Janning, 1998.8.10) presumptive embryonic/larval peripheral nervous system   (Jackle and Janning, 1998.8.10) embryonic/larval salivary gland   (Jackle and Janning, 1998.8.10) embryonic/larval hindgut   (Jackle and Janning, 1998.8.10) embryonic labral segment   (Jackle and Janning, 1998.8.10) proctodeum   (Jackle and Janning, 1998.8.10) embryonic stage 6 -- 10   organism | segment polarity expression pattern   (Eldon et al., 1994) embryonic stage 11   salivary gland body primordium   (Eldon et al., 1994) tracheal pit   (Eldon et al., 1994) embryonic stage 14 -- 16   central nervous system | restricted   (Eldon et al., 1994) anal pad   (Eldon et al., 1994) embryonic/larval tracheal system   (Eldon et al., 1994) embryonic/larval dorsal vessel   (Eldon et al., 1994) embryonic/larval salivary gland   (Eldon et al., 1994) embryonic/larval hindgut   (Eldon et al., 1994) embryonic/larval pharynx   (Eldon et al., 1994) embryonic/larval posterior spiracle   (Eldon et al., 1994) embryonic/larval muscle system | ventral   (Eldon et al., 1994) adult stage   third segment of antenna   (Vosshall, 1998.8.10) medulla   (Vosshall, 1998.8.10) lamina   (Vosshall, 1998.8.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval trachea, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult hindgut, adult heart, adult fat body, adult salivary gland, adult ovary, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea    864.2 Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca    622.1 Adult InseminatedFemale Spermatheca    653.4 Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea  (864.2) Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca  (622.1) Adult InseminatedFemale Spermatheca  (653.4) Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea    864.2 Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca    622.1 Adult InseminatedFemale Spermatheca    653.4 Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea    864.2 Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca    622.1 Adult InseminatedFemale Spermatheca    653.4 Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea    864.2 Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca    622.1 Adult InseminatedFemale Spermatheca    653.4 Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea  (864.2) Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca  622.1 Adult InseminatedFemale Spermatheca  653.4 Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea    864.2 Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca    622.1 Adult InseminatedFemale Spermatheca    653.4 Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    261.35 Larval Midgut    59 Larval Hindgut    153.8 Larval Malpighian Tubules    29.9 Larval Fat Body    55.8 Larval Salivary Gland    48.4 Larval Trachea    864.2 Larval Carcass    120.4 Adult Head    400.3 Adult Eye    330.05 Adult Brain    287.8 Adult Thoracic-Abdominal Ganglion    316 Adult Crop    77.2 Adult Midgut    57.5 Adult Hindgut    249.2 Adult Malpighian Tubules    21.1 Adult Fat Body    339.2 Adult Salivary Gland    295.5 Adult Heart    296.7 Adult VirginFemale Spermatheca    622.1 Adult InseminatedFemale Spermatheca    653.4 Adult Ovary    116.2 Adult Testis    39.2 Adult Male Accessory Gland    35.7 Adult Carcass    345.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    400.3   NA  Eye    330.05   NA  Brain    287.8   261.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    316   NA  Crop    77.2   59  Midgut    57.5   153.8  Hindgut    249.2   29.9  Malpighian Tubules    no informative data   55.8  Fat Body    339.2   48.4  Salivary Gland    295.5   NA  Heart    296.7   864.2  Trachea    NA   NA  VirginFemale Spermatheca    622.1   NA  InseminatedFemale Spermatheca    653.4   NA  Ovary    116.2   NA  Testis    39.2   NA  Male Accessory Gland    35.7   120.4  Carcass    345.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering    28 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (32) central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering  (28) carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering    28 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering    28 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering    28 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  32 central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering  28 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering    28 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    17 central nervous system, pupae P8    20 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    17 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    13 head, mated 1-day male    18 head, mated 4-day male    16 head, mated 20-day male    19 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    11 carcass, larvae L3 wandering    28 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    44 embryo 04-06hr    83 embryo 06-08hr    86 embryo 08-10hr    75 embryo 10-12hr    46 embryo 12-14hr    47 embryo 14-16hr    50 embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr    26 white prepupae 24hr    37 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr  (44) embryo 04-06hr  (83) embryo 06-08hr  (86) embryo 08-10hr  (75) embryo 10-12hr  (46) embryo 12-14hr  (47) embryo 14-16hr  (50) embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr  26 white prepupae 24hr  (37) pupae 2d postWPP  (33) pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    44 embryo 04-06hr    83 embryo 06-08hr    86 embryo 08-10hr    75 embryo 10-12hr    46 embryo 12-14hr    47 embryo 14-16hr    50 embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr    26 white prepupae 24hr    37 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    44 embryo 04-06hr    83 embryo 06-08hr    86 embryo 08-10hr    75 embryo 10-12hr    46 embryo 12-14hr    47 embryo 14-16hr    50 embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr    26 white prepupae 24hr    37 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    44 embryo 04-06hr    83 embryo 06-08hr    86 embryo 08-10hr    75 embryo 10-12hr    46 embryo 12-14hr    47 embryo 14-16hr    50 embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr    26 white prepupae 24hr    37 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr  (44) embryo 04-06hr  (83) embryo 06-08hr  (86) embryo 08-10hr  (75) embryo 10-12hr  (46) embryo 12-14hr  (47) embryo 14-16hr  (50) embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr  26 white prepupae 24hr  (37) pupae 2d postWPP  (33) pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    44 embryo 04-06hr    83 embryo 06-08hr    86 embryo 08-10hr    75 embryo 10-12hr    46 embryo 12-14hr    47 embryo 14-16hr    50 embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr    26 white prepupae 24hr    37 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    44 embryo 04-06hr    83 embryo 06-08hr    86 embryo 08-10hr    75 embryo 10-12hr    46 embryo 12-14hr    47 embryo 14-16hr    50 embryo 16-18hr    25 embryo 18-20hr    15 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    8 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    23 white prepupae 12hr    26 white prepupae 24hr    37 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    10 adult male 30day    8 adult female 01day    12 adult female 05day    8 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    36 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8  (36) wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS  26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    36 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    36 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    36 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8  (36) wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS  26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    36 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    0 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    0 embryonic S1    9 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    36 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    24 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    26 ovary OSC    20 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	BDGP expression data • GH23463 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004364 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8896-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal 18wk02701 18wΔ7-35 lethal, with Scer\GAL432B 18wg.Scer\UAS lethal, with Scer\GAL4Act.PU 18wGD14418 lethal, with Scer\GAL4da.G32 18wg.Scer\UAS lethal, with Scer\GAL4e22c 18wg.Scer\UAS lethal, with Scer\GAL4ptc-559.1 18wg.Scer\UAS lethal | larval stage | recessive 18wΔ1-82 18wΔ7-35 lethal | recessive 18w00053 18wk02701 18wΔ1-11 18wΔ1-14 18wΔ1-15 18wΔ1-24 18wΔ2-62 18wΔ2-63 18wΔ6-81 18wΔ7-35 18wΔ7-43 partially lethal - majority die (with 18wk02701) 18wΔ7-35 partially lethal - majority die (with 18wΔ7-35) 18wk02701 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 18wGD14418 viable 18wΔ1-12 viable, with Scer\GAL4pnr-MD237 18wGD17 18wGD2513 viable (with Df(2R)017) 18wΔ7-35 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 18wGD14418 immune response defective | recessive 18wΔ7-35 visible, with Scer\GAL4pnr-MD237 18wGD14418 wild-type 18wΔ1-12 Phenotype manifest in Allele adult abdomen, with Scer\GAL471B 18wg.Scer\UAS antenna 18wΔ1-82 18wΔ7-35 eye, with Scer\GAL471B 18wg.Scer\UAS eye, with Scer\GAL4GMR.PF 18wg.Scer\UAS eye, with Scer\GAL4lz-gal4 18wg.Scer\UAS haltere 18wΔ1-82 18wΔ7-35 leg 18wΔ1-82 18wΔ7-35 leg, with Scer\GAL4dpp.PS 18wg.Scer\UAS macrochaeta | ectopic, with Scer\GAL471B 18wg.Scer\UAS mesothoracic tergum, with Scer\GAL4pnr-MD237 18wGD14418 ventral thoracic disc, with Scer\GAL4dpp.PS 18wg.Scer\UAS wing 18wΔ1-82 18wΔ7-35 wing, with Scer\GAL4dpp.PS 18wg.Scer\UAS wing disc, with Scer\GAL4dpp.PS 18wg.Scer\UAS
Classical Alleles ( 14 )
For All Classical Alleles Show
Allele of 18w Class Mutagen Stocks Known lesion 18wk02701 P-element activity 2 -- 18wΔ7-35 loss of function allele Delta2-3 P-element activity 2 Yes 18w00053 hypomorphic allele - genetic evidence P-element activity 0 Yes 18wunspecified 0 -- 18wΔ1-11 loss of function allele Delta2-3 0 Yes 18wΔ1-12 wild-type allele Delta2-3 P-element activity 0 Yes 18wΔ1-14 loss of function allele Delta2-3 0 Yes 18wΔ1-15 loss of function allele Delta2-3 0 Yes 18wΔ1-24 loss of function allele Delta2-3 0 Yes 18wΔ1-82 loss of function allele Delta2-3 0 Yes 18wΔ2-62 loss of function allele Delta2-3 0 Yes 18wΔ2-63 loss of function allele Delta2-3 0 Yes 18wΔ6-81 loss of function allele Delta2-3 0 Yes 18wΔ7-43 loss of function allele Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of 18w Class Mutagen Stocks Known lesion 18wGD17 in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes 18wGD2513 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes 18wHM05241 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes 18wfl.cEa in vitro construct 0 Yes 18wg.Scer\UAS 0 Yes 18wGD14418 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes 18wNIG.8896R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes 18wScer\UAS.cRa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)017 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)BSC22 (Deal-Herr and Cook, 2002.9.10) Df(2R)BSC594 (Christensen et al., 2008.9.3)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD17} NA P{GD2513} NA P{GD14418} NA P{NIG.8896R} NA P{TRiP.HM05241} NA P{UAS-18w.g} NA P{UAS-18w.R} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{A92}Q26b No P{lacW}18wk02701 No P{lacW}l(2)SH1382SH1382 No P{PZ}18w00053 No insertion of mobile activating element P{GSV1}EP-709EP-709 NA
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
anatomical structure morphogenesis inferred from mutant phenotype (Eldon et al., 1994) antibacterial humoral response inferred from mutant phenotype (Williams et al., 1997) NOT antimicrobial humoral response inferred from mutant phenotype (Ligoxygakis et al., 2002) cell adhesion inferred from direct assay (Eldon et al., 1994) immune response inferred from expression pattern (Williams et al., 1997)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Williams et al., 1997) plasma membrane inferred from direct assay (Williams et al., 1997)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
transmembrane signaling receptor activity inferred from sequence or structural similarity (Lemaitre, 2000.12.20) inferred from sequence or structural similarity with Tl (Eldon et al., 1994) inferred from sequence or structural similarity with Toll-7 (Mi et al., 2001.12.13)
Biological Process
CV term Evidence References
antibacterial humoral response non-traceable author statement (Lemaitre, 2000.12.20) traceable author statement (Kimbrell and Beutler, 2001) defense response traceable author statement (Meister et al., 2000) embryonic morphogenesis traceable author statement (Meister et al., 2000) multicellular organismal development traceable author statement (Kimbrell and Beutler, 2001) signal transduction inferred from electronic annotation with InterPro:IPR000157, InterPro:IPR004075 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Lemaitre, 2000.12.20)
Cellular Component
CV term Evidence References
integral to membrane inferred from sequence or structural similarity (Lemaitre, 2000.12.20) plasma membrane inferred from sequence or structural similarity with Tl (Eldon et al., 1994)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions 18w allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62933 DroID - A comprehensive database of gene and protein interactions. • FBgn0004364 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37277
Orthologs
OrthoDB Orthologs (92) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG64MZBB Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG63JB0N Arthropod inclusive ortholog search EOG6931ZS Metazoa inclusive ortholog search EOG676XS2
Orthologs in Drosophila Species (EOG64MZBB)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\18w     Drosophila sechellia   Dsec\GM21990 Y   Drosophila erecta   Dere\GG22011 Y   Drosophila yakuba   Dyak\18w Y   Drosophila ananassae   Dana\GF11743 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21398 Y   Drosophila persimilis   Dper\GL10366 Y   Drosophila willistoni   Dwil\GK15901 Y   Drosophila virilis   Dvir\GJ20945 Y   Drosophila mojavensis   Dmoj\GI21098 Y   Drosophila grimshawi   Dgri\GH23027 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG63JB0N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\18w Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007768 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007767 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14297 Y Atta cephalotes Leafcutter ant  Acep\ACEP18848 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17241 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL11271 Y Linepithema humile Argentine ant  Lhum\LH13356 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB13114 Y Solenopsis invicta Red fire ant  Sinv\SINV17709 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008698 Y Bombyx mori Silkmoth  Bmor\Bm18w Y Bombyx mori Silkmoth  Bmor\BGIBMGA011038 Y Pediculus humanus Human body louse  Phum\PHUM480550 Y Tribolium castaneum Red flour beetle  Tcas\TC004474 Y
Orthologs in non-Insect Arthropods (EOG6931ZS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_65779 Y Daphnia pulex Water flea  Dpux\Slc6a9 Y Ixodes scapularis Deer tick  Isca\ISCW022740 Y Ixodes scapularis Deer tick  Isca\ISCW020989 Y
Orthologs in non-Arthropod Metazoa (EOG676XS2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\tol-1 Y Caenorhabditis elegans Nematode  Cele\lron-1 Y Caenorhabditis elegans Nematode  Cele\lron-11 Y Caenorhabditis elegans Nematode  Cele\egg-6 Y Caenorhabditis elegans Nematode  Cele\lron-5 Y Caenorhabditis elegans Nematode  Cele\lron-6 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000428gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001676gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006951gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004360gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025731gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014928gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012666gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028380gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008227gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018608gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007859gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017640gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020527gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005148gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006952gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024731gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020257gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_030131gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013824gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020428gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025966gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000871gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024156gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000425gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012819gn Y Danio rerio Zebrafish  Drer\si:ch211-117l17.7 Y Danio rerio Zebrafish  Drer\ENSDARG00000089040 Y Danio rerio Zebrafish  Drer\ENSDARG00000063518 Y Danio rerio Zebrafish  Drer\lrrc15 Y Danio rerio Zebrafish  Drer\tlr21 Y Danio rerio Zebrafish  Drer\si:dkey-90m5.4 Y Danio rerio Zebrafish  Drer\si:ch211-66h3.1 Y Xenopus tropicalis Western clawed frog  Xtro\igfals Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000004577 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000032848 Y Xenopus tropicalis Western clawed frog  Xtro\lrrc15 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000030284 Y Xenopus tropicalis Western clawed frog  Xtro\gp5 Y Gallus gallus Domestic chicken  Ggal\TLR21 Y Gallus gallus Domestic chicken  Ggal\IGFALS Y Gallus gallus Domestic chicken  Ggal\LRRC15 Y Mus musculus House mouse  Mmus\Gp5 Y Mus musculus House mouse  Mmus\Lrg1 Y Mus musculus House mouse  Mmus\Lrrc15 Y Mus musculus House mouse  Mmus\Tlr13 Y Mus musculus House mouse  Mmus\Igfals Y Mus musculus House mouse  Mmus\Cpn2 Y Rattus norvegicus Norway rat  Rnor\Gp5 Y Rattus norvegicus Norway rat  Rnor\Cpn2 Y Rattus norvegicus Norway rat  Rnor\Igfals Y Rattus norvegicus Norway rat  Rnor\RGD1562655 Y Homo sapiens Human  Hsap\IGFALS Y Homo sapiens Human  Hsap\LRRC15 Y Homo sapiens Human  Hsap\CPN2 Y Homo sapiens Human  Hsap\GP5 Y Homo sapiens Human  Hsap\LRG1 Y
Human Orthologs (5)
Gene OMIM HGNC Hsap\IGFALS     Hsap\LRRC15     Hsap\CPN2     Hsap\GP5     Hsap\LRG1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\18w Drosophila sechellia Dsec\GM21990 Drosophila erecta Dere\GG22011 Drosophila yakuba Dyak\18w Drosophila ananassae Dana\GF11743 Drosophila pseudoobscura pseudoobscura Dpse\GA21398 Drosophila persimilis Dper\GL10366 Drosophila willistoni Dwil\GK15901 Drosophila virilis Dvir\GJ20945 Drosophila mojavensis Dmoj\GI21098 Drosophila grimshawi Dgri\GH23027
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	4372 y1 w*; 18wΔ7-35/CyO 10518 y1 w67c23; P{lacW}18wk02701/CyO 30498 y1 sc* v1; P{TRiP.HM05241}attP2/TM3, Sb1
Kyoto	107712 y1 w*; 18wΔ7-35/CyO 111152 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}18wk02701 /CyO y+
VDRC	v963 w1118; P{GD17}v963 v965 w1118; P{GD17}v965 v44387 w1118; P{GD2513}v44387 v966 w1118; P{GD17}v966/CyO v44386 w1118; P{GD2513}v44386/TM3
Genomic Clones ( 2 )
	BACR20D06 BACR23F24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 18 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH23463
Other clones	RT09901
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM05705
Other clones	1304 36020 41569 52242 82155 214449.69 214507.68 CK01.146A.G6 EK054009 EK119959 EK122354 EK154751 EK166861 EP03221 EP09026
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004364 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37277
Antibody Information
	polyclonal (Eldon et al., 1994)
Other Information
Discoverer
Etymology
	Named "18 wheeler" on basis of 18 stripes of expression during early gastrulation. (Eldon et al., 1994)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: 18w CG8896 (Milshina, 1999.11)
Source for database merge of
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Pilot et al., 2006) Loss of function 18w mutant follicle cells exhibit delayed migration. The eggs laid by feamles with ovarian clones show defects in egg shape and dorsal appendage morphology. (Siler et al., 2006) Mutant allele fails to complement a QTL affecting male mating behaviour. (Moehring and Mackay, 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) 18w is a critical component of the humoral immune response. (Williams et al., 1997) 18w protein is 1389 amino aids in length. (Eldon, 1995) The 18w protein functions as a receptor mediating intercellular communication during various developmental events, including pattern formation, imaginal cell determination and the larval immune response. Expression of 18w protein in non-adhesive Schneider 2 cells promotes heterophilic cell aggregation as seen in similar experiments with Tl and other receptor-ligand molecules. (Williams et al., 1995) 18w encodes a protein that contains multiple leucine rich motifs (LRR) in its presumed extracellular domain. 18w may be involved in cell-to-cell interactions. (Chiang and Beachy, 1994) Molecular and phenotypic analysis suggests 18w participates in the developmental program specified by segmentation and homeotic genes as a cell adhesion or receptor molecule that facilitates cell movements. (Eldon et al., 1994) 18w open reading frame encodes a protein with significant similarities to both Tl and chp. (Eldon et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057466 RefSeq (Proteins) • NP_476814 DDBJ / EMBL / GenBank • AAA79208 AAB33383 AAF57509 AAK93016 ABA86544 AC004428 AC005286 AI403917 AQ025714 AY051592 DQ138938 L23171 S76155 Entrez Gene - A searchable database of RefSeq genes. • 37277 UniProtKB/TrEMBL • A1ZBR2 Q2XXS4 Q7JPR9 Q961H0 Q24591
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Toll/interleukin-1 receptor homology (TIR) domain (IPR000157) Cysteine-rich flanking region, C-terminal (IPR000483) Leucine-rich repeat (IPR001611) Leucine-rich repeat, typical subtype (IPR003591) Leucine-rich repeat-containing N-terminal (IPR000372) Leucine rich repeat 5 (IPR026906) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH23463
Linkouts
	BioGRID - A database of protein and genetic interactions • 62933 DroID - A comprehensive database of gene and protein interactions. • FBgn0004364 DRSC - Results from RNAi screens. • FBgn0004364 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004364 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8896-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004364 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37277 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/eghtnw1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37277 modMine - Data generated by the modENCODE project. • FBgn0004364
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	18W (Solano et al., 2003, Tauszig et al., 2000, Kolesnikov and Beckendorf, 2007) 18w (Siler et al., 2006, Sandmann et al., 2007, Gilchrist et al., 2010, Mace et al., 2005, Gim et al., 2001, Sandoval and Eldon, 2008, Vielmas et al., 2008, Molnar et al., 2006, Lazzaro et al., 2004, Christensen et al., 2008.9.3, Kleve et al., 2006, Yagi et al., 2010, Mudiganti et al., 2010, Yakoby et al., 2008, Ligoxygakis et al., 2002, Ruedi and Hughes, 2009, Sato et al., 2010, Tauszig-Delamasure et al., 2002, Haussmann et al., 2008, Cunha et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Felix et al., 2012) 18-w (Díaz-Castillo et al., 2012) 18-wheeler (Wagner et al., 2008) CG8896 (Perkins et al., 2009, Molnar et al., 2006, Haussmann et al., 2008) CT25100 (Hynes and Zhao, 2000) l(2)00053   tlr (Chiang, 1995.7.26, Chiang and Beachy, 1994) toll (Janssens and Beyaert, 2002) Toll-2 (Nakamoto et al., 2012)
Name Synonym	18-wheeler (Swanson et al., 2009, Mace et al., 2005, Wasserman, 2000, Nappi and Ottaviani, 2000, Botas and Auwers, 1996, Siler et al., 2006, Lazzaro et al., 2006, Kurusu et al., 2008, Kleve et al., 2006, Tauszig-Delamasure et al., 2002) 18 Wheeler (Lemaitre, 2000.12.20, Wang et al., 2006, Kolesnikov and Beckendorf, 2007) 18-Wheeler (Janeway and Medzhitov, 1999, Lazzaro et al., 2004, Ligoxygakis et al., 2002) 18 wheeler (Pilot et al., 2006, Gim et al., 2001, Ruedi and Hughes, 2009) 18Wheeler (Ooi et al., 2002, Yagi et al., 2010) 18wheeler (Haussmann et al., 2008) Toll-2 (Sandoval and Eldon, 2008, Christophides et al., 2002, Ooi et al., 2002) Toll like receptor (Chiang and Beachy, 1994) toll receptor (Haesemeyer M et al., 2007) wheeler (Eldon, 1993)
Secondary FlyBase IDs
References ( 133 )
Generate a list of	All references relating to this gene ( 133 )
List References by type	Research paper  ( 53 ) Supplementary material  ( 7 ) Review  ( 27 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 19 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 5 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 1 )
Recent research papers ( 7 )
	Díaz-Castillo et al., 2012, PLoS Genet. 8(2): e1002475 Evaluation of the role of functional constraints on the integrity of an ultraconserved region in the genus Drosophila. [FBrf0217446] Felix et al., 2012, Genetics 191(3): 989--1002 Age-Specific Variation in Immune Response in Drosophila melanogaster Has a Genetic Basis. [FBrf0218944] Junion et al., 2012, Cell 148(3): 473--486 A transcription factor collective defines cardiac cell fate and reflects lineage history. [FBrf0217404] Nakamoto et al., 2012, Immunity 36(4): 658--667 Virus recognition by toll-7 activates antiviral autophagy in Drosophila. [FBrf0218107] Weber et al., 2012, PLoS ONE 7(4): e34745 Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073] Xu et al., 2012, Cell Host Microbe 12(4): 531--543 Transcriptional pausing controls a rapid antiviral innate immune response in Drosophila. [FBrf0219695] Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...