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FB2025_02
,
released April 17, 2025
DPTP10D
receptor tyrosine phosphatase - required for embryonic and larval axon guidance - along with Ptp69D regulates segregation of the young axons into a single core bundle in the larval mushroom body - mutants are defective in long-term memory formation - Ptp10D-Ptp69D double mutants have a strong phenotype in which embryonic CNS axons abnormally cross the ventral midline
Please see the JBrowse view of Dmel\Ptp10D for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Stop-codon suppression (UGA) postulated; FBrf0216884.
Transposon inserted in intron
Gene model reviewed during 5.44
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
8.2, 7.1, 6.3 (northern blot)
7.8, 6.5, 5.6 (northern blot)
1558 (aa); 177 (kD predicted)
1631, 1558 (aa); 185, 177 (kD)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ptp10D using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: reference states 9-18 hr AEL
In stage 16 embryos, Ptp10D antibody brightly stains axons in the ventral nerve cord (VNC) and brain, but body wall staining is weak and has no clear pattern.
Localized Ptp10D protein is first observed in the axonal layer in stage 12 embryos. By stage 14 expression is seen along the commissures and longitudinal connectives. The junctions between the anterior commissures and the longitudinal connectives are heavily stained and the posterior commissures stain more weakly than the anterior commissures. By stage 15, expression is uniform on all axons. Staining of trachea and salivary glands is also observed.
Ptp10D staining is first observed in the CNS at the end of stage 12. In stage 13, expression is seen in cell bodies (~8 cells/hemisegment) and in extending neuronal processes. Staining of longitudinal connectives is more intense than staining of commissures and no staining of the anterior and posterior fascicles is seen. Expression in the commissures fades before the expression in the longitudinal connectives.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Ptp10D in JBrowse1-35
1-36.7
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
polyclonal
New stable cell line derived from S2-unspecified : Stable cell lines were generated starting from a S2+ line that stably expresses Egfr. This line was stably transformed with full length or mutant Ptp10D.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Spatial and temporal transcript accumulation pattern in ovaries is determined by in situ hybridisation.
Transmembrane Gp150 glycoprotein selectively interacts with the active site of the catalytic domain of Ptp10D (interaction is blocked by mutation of the active site or by the inhibitor vanadate). Ptp10D may function in vivo to regulate phosphorylation of Gp150 thereby controlling interactions with downstream effectors.
Receptor linked PTPases may be involved in axon outgrowth and guidance during embryonic development.
Source for identity of: Ptp10D CG1817
