Stop-codon suppression (UGA) postulated; FBrf0216884.
Transposon inserted in intron
gene_with_stop_codon_read_through ; SO:0000697
Gene model reviewed during 5.44
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ptp10D using the Feature Mapper tool.
In stage 16 embryos, Ptp10D antibody brightly stains axons in the ventral nerve cord (VNC) and brain, but body wall staining is weak and has no clear pattern.
Localized Ptp10D protein is first observed in the axonal layer in stage 12 embryos. By stage 14 expression is seen along the commissures and longitudinal connectives. The junctions between the anterior commissures and the longitudinal connectives are heavily stained and the posterior commissures stain more weakly than the anterior commissures. By stage 15, expression is uniform on all axons. Staining of trachea and salivary glands is also observed.
Ptp10D staining is first observed in the CNS at the end of stage 12. In stage 13, expression is seen in cell bodies (~8 cells/hemisegment) and in extending neuronal processes. Staining of longitudinal connectives is more intense than staining of commissures and no staining of the anterior and posterior fascicles is seen. Expression in the commissures fades before the expression in the longitudinal connectives.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Ptp10D in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Ptp10D CG1817
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Ptp10D and shg form stable complexes in culture cells and in wild type larval tissue. Ptp10D cytoplasmic domain is sufficient to confer binding to shg. Results support the hypothesis that Ptp10D regulates shg signaling in vivo.
Spatial and temporal transcript accumulation pattern in ovaries is determined by in situ hybridisation.
Transmembrane Gp150 glycoprotein selectively interacts with the active site of the catalytic domain of Ptp10D (interaction is blocked by mutation of the active site or by the inhibitor vanadate). Ptp10D may function in vivo to regulate phosphorylation of Gp150 thereby controlling interactions with downstream effectors.
Receptor linked PTPases may be involved in axon outgrowth and guidance during embryonic development.