FB2025_01 , released February 20, 2025
Gene: Dmel\Klp61F
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General Information
Symbol
Dmel\Klp61F
Species
D. melanogaster
Name
Kinesin-like protein at 61F
Annotation Symbol
CG9191
Feature Type
protein_coding_gene
FlyBase ID
FBgn0004378
Gene Model Status
Current
Stock Availability
16 publicly available
Enzyme Name (EC)
plus-end-directed kinesin ATPase (5.6.1.3)
Gene Summary
Kinesin-like protein at 61F (Klp61F) encodes a member of the kinesin-5 family of cytoskeletal motor proteins. It allows the crosslinking and sliding apart of adjacent microtubules. This 'sliding filament mechanism' is critical for many aspect of mitosis and chromosome segregation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

kinesin-5, urchin, KRP130, DmKlp61F, KIF11

Key Links
Genomic Location
Cytogenetic map
61F4-61F4
Sequence location
Recombination map
3-0
RefSeq locus
NT_037436 REGION:1241568..1245910
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (34 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
enables cytoskeletal motor activity
inferred from direct assay
(Cole and Scholey, 1995)
enables microtubule binding
inferred from direct assay
(Barton et al., 1995)
enables plus-end-directed microtubule motor activity
inferred from direct assay
(Barton et al., 1995)
enables protein binding
inferred from physical interaction with UniProtKB:P54350
(Garcia et al., 2009)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821
(InterPro Project Members, 2004-)
enables microtubule binding
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR025901
(InterPro Project Members, 2004-)
enables microtubule motor activity
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821
(InterPro Project Members, 2004-)
enables plus-end-directed microtubule motor activity
inferred from biological aspect of ancestor with PANTHER:PTN000649234
(GO Reference Genome Project, 2011-)
Biological Process (13 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
involved_in centrosome separation
inferred from mutant phenotype
(Gandhi et al., 2004)
involved_in fusome organization
inferred from mutant phenotype
(Wilson, 1999)
involved_in Golgi organization
inferred from mutant phenotype
(Bard et al., 2006)
involved_in microtubule bundle formation
inferred from direct assay
(Tao et al., 2006)
involved_in microtubule-based movement
inferred from direct assay
(Barton et al., 1995)
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
(Ducat et al., 2008)
involved_in mitotic centrosome separation
inferred from mutant phenotype
(Sharp et al., 1999)
inferred from genetic interaction with FLYBASE:ncd; FB:FBgn0002924
(Sharp et al., 1999)
involved_in mitotic spindle organization
inferred from mutant phenotype
(Garcia et al., 2009, Goshima and Vale, 2005)
involved_in plus-end directed microtubule sliding
inferred from direct assay
(Tao et al., 2006)
involved_in positive regulation of Golgi to plasma membrane protein transport
inferred from mutant phenotype
(Wakana et al., 2013)
involved_in protein secretion
inferred from mutant phenotype
(Bard et al., 2006)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in microtubule-based movement
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821
(InterPro Project Members, 2004-)
involved_in mitotic spindle assembly
inferred from biological aspect of ancestor with PANTHER:PTN000649234
(GO Reference Genome Project, 2011-)
involved_in spindle elongation
inferred from biological aspect of ancestor with PANTHER:PTN000649234
(GO Reference Genome Project, 2011-)
Cellular Component (15 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
located_in aster
inferred from direct assay
(Wilson, 1999)
colocalizes_with centrosome
inferred from direct assay
(Cheerambathur et al., 2008)
located_in cytoplasm
inferred from direct assay
(Goshima and Vale, 2005, Cole and Scholey, 1995)
inferred from direct assay
(Sharp et al., 1999, Wilson, 1999)
located_in endoplasmic reticulum
inferred from direct assay
(Bard et al., 2006)
located_in fusome
inferred from direct assay
(Wilson, 2005)
inferred from direct assay
(Wilson, 1999)
located_in Golgi apparatus
inferred from direct assay
(Bard et al., 2006)
part_of kinesin complex
inferred from direct assay
(Cole and Scholey, 1995)
part_of microtubule associated complex
inferred from direct assay
(Cole and Scholey, 1995)
located_in microtubule
inferred from direct assay
(Cheerambathur et al., 2008)
located_in mitotic spindle microtubule
inferred from direct assay
(Goshima and Vale, 2005)
located_in mitotic spindle pole
inferred from direct assay
(Barton et al., 1995)
located_in mitotic spindle
inferred from direct assay
(Garcia et al., 2009, Sharp et al., 1999, Wilson, 1999, Barton et al., 1995)
located_in nucleus
inferred from direct assay
(Sharp et al., 1999)
located_in spindle
inferred from direct assay
(Cheerambathur et al., 2008)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in mitotic spindle
inferred from biological aspect of ancestor with PANTHER:PTN000649234
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000649235
(GO Reference Genome Project, 2011-)
is_active_in spindle microtubule
inferred from biological aspect of ancestor with PANTHER:PTN000649234
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily. (P46863)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
plus-end-directed microtubule motor activity
ATP + H2O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end) (5.6.1.3)
Summaries
Gene Snapshot
Kinesin-like protein at 61F (Klp61F) encodes a member of the kinesin-5 family of cytoskeletal motor proteins. It allows the crosslinking and sliding apart of adjacent microtubules. This 'sliding filament mechanism' is critical for many aspect of mitosis and chromosome segregation. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
KINESINS -
Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).
Protein Function (UniProtKB)
Important role in mitotic dividing cells (PubMed:8227131). Microtubule motor required for spindle body separation (PubMed:8918872). Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function (PubMed:19062285, PubMed:8589456, PubMed:8918872). Forms cross-links between microtubules within interpolar microtubule bundles (PubMed:19158379, PubMed:9885249). Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates (PubMed:19158379). Displays microtubule-stimulated ATPase activity (PubMed:8589456). Required for normal fusome organization (PubMed:10469596). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
(UniProt, P46863)
Summary (Interactive Fly)

motor protein - crosslinking and sliding activities during mitosis facilitate accumulation within antiparallel interpolar MTs at the equator, where the motor generates force to drive spindle poleward flux and pole-pole separation

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Klp61F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P46863)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Klp61F-RA
FBtr0072733
NM_057470
3871
1066
Additional Transcript Data and Comments
Reported size (kB)

3.7 (longest cDNA)

(Heck et al., 1993)

3.9, 3.7 (northern blot)

(Stewart et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Klp61F-PA
FBpp0072616
121.2
1066
6.39
P46863
NP_476818
AAF47458
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

120 (kD observed)

(Barton et al., 1995)

130 (kD observed)

(Cole et al., 1994)

1066 (aa)

(Heck et al., 1993)
Comments
External Data
Subunit Structure (UniProtKB)

Homotetramer (PubMed:24714498, PubMed:8538794, PubMed:9885249). Consists of two pairs of polypeptides associated by coiled-coil interactions to form two homodimers (PubMed:8538794). The homodimers are linked by lateral interactions between their coiled-coil regions to form a bipolar homotetramer consisting of a central rod with two motor domains projecting from either end (PubMed:8538794). Parallel coiled coils extend from each pair of motor heads, switch to two antiparallel coiled coils in the central region and then back to parallel coiled coils (PubMed:24714498). Interacts with Wee1 (PubMed:19800237).

(UniProt, P46863)
Post Translational Modification

Phosphorylation is required for localization to mitotic spindles (PubMed:9885249). Phosphorylation of Thr-933 during mitosis controls association with the spindle apparatus (By similarity). Phosphorylated in vitro by Wee1 (PubMed:19800237).

(UniProt, P46863)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp61F using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.02

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ventral midline of embryo
(Heck et al., 1993)
cephalic furrow
(Heck et al., 1993)
ventral nerve cord | restricted
(Heck et al., 1993)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 6
mesoderm anlage
(Heck et al., 1993)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 9 -- 12
midline primordium | subset
(Kearney et al., 2004)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
lateral cord glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
embryonic central brain surface glial cell
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
embryonic/larval lymph gland
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
ventral sensory complex primordium
(Fisher et al., 2012)
embryonic stage 17
germline cell
(Casper and Van Doren, 2009)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage & larval stage & pupal stage
(Stewart et al., 1991)
adult stage | female
ovary
(Stewart et al., 1991)
adult stage | male
testis
(Stewart et al., 1991)
Additional Descriptive Data

Klp61F transcripts were detected throughout the cytoplasm of unfertilized eggs. The maternal RNA persists until cellularization. During gastrulation, Klp61F transcripts are first detected in a wide band of ventral cells corresponding to the invaginating presumptive mesoderm. They are also seen in a more dorsal strip of cells and underneath the pole cells. Later embryos show expression in the head, along the cephalic furrow, along the ventral midline, and within the posterior region. After germ band retraction, expression is limited to the cells of the head and ventral neurogenic region. Regions of expression are well correlated with domains of cell division.

(Heck et al., 1993)

Klp61F transcripts are expressed throughout development. In adults, they are detected only in the gonads.

(Stewart et al., 1991)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data

Throughout mitosis, nonphosphorylated Klp61F protein is diffuse and cytoplasmic. Phosphorylated Klp61F shows a very different distribution. During interphase and prophase, the staining is punctate and concentrated in the nucleus. Occasional faint staining is seen in the centrosome in prophase. By metaphase, the staining localizes to the spindle and appears filamentous. Phospho-Klp61F is particularly concentrated on the half spindles and densely stained bridges between the spindles in the midzone. Lower level staining occurs at the oles and grainy cytoplasmic staining is observed. During anaphase A, the staining resembles metaphase. During anaphase B, nearly all the phospho-Klp61F is present in the spindle between the separating nuclei. Filaments of phospho-Klp61F protein staining cross the midzone while little or no staining is associated with the spindle poles and astral microtubules. During telophase, Klp61F staining is most pronounced on two adjacent patches on the remnants of the central spindle. EM studies suggest that the phospho-Klp61F motors form crosslinks between microtubules within interpolar microt bule bundles.

(Sharp et al., 1999)

Klp61F protein localizes to the structural domains of the mitotic spindle throughout mitosis.

(Barton et al., 1995)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in aster
inferred from direct assay
(Wilson, 1999)
colocalizes_with centrosome
inferred from direct assay
(Cheerambathur et al., 2008)
located_in cytoplasm
inferred from direct assay
(Goshima and Vale, 2005, Cole and Scholey, 1995)
inferred from direct assay
(Sharp et al., 1999, Wilson, 1999)
located_in endoplasmic reticulum
inferred from direct assay
(Bard et al., 2006)
located_in fusome
inferred from direct assay
(Wilson, 2005)
inferred from direct assay
(Wilson, 1999)
located_in Golgi apparatus
inferred from direct assay
(Bard et al., 2006)
part_of kinesin complex
inferred from direct assay
(Cole and Scholey, 1995)
part_of microtubule associated complex
inferred from direct assay
(Cole and Scholey, 1995)
located_in microtubule
inferred from direct assay
(Cheerambathur et al., 2008)
located_in mitotic spindle microtubule
inferred from direct assay
(Goshima and Vale, 2005)
located_in mitotic spindle pole
inferred from direct assay
(Barton et al., 1995)
located_in mitotic spindle
inferred from direct assay
(Garcia et al., 2009, Sharp et al., 1999, Wilson, 1999, Barton et al., 1995)
located_in nucleus
inferred from direct assay
(Sharp et al., 1999)
located_in spindle
inferred from direct assay
(Cheerambathur et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Klp61F in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Klp61F
Transgenic constructs containing regulatory region of Klp61F
Aberrations (Deficiencies and Duplications) ( 9 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
lethal, with Scer\GAL4insc-Mz1407
lethal, with Scer\GAL4Tub.PU
lethal, with Scer\GAL4Ubi.PU
lethal (with Df(3L)3C7)
lethal (with Df(3L)bab-PG)
lethal | recessive
lethal - all die before end of larval stage (with Klp61F06836)
lethal - all die before end of larval stage (with Klp61F07012)
lethal - all die before end of larval stage | recessive
lethal - all die before end of pupal stage | recessive
lethal - all die before end of second instar larval stage | recessive
lethal - all die during embryonic stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
lethal - all die during embryonic stage | recessive
viable, with Klp61F06836/Klp61F07012
viable, with Klp61F07012
viable, with Klp61FUbi-p63E.GFP
viable, with Scer\GAL4tin.CΔ4
viable (with Klp61F06836), with Klp61FUbi-p63E.GFP
viable (with Klp61F07012), with Klp61FUbi-p63E.GFP
Sterility
Allele
fertile, with Klp61F06836/Klp61F07012
fertile, with Klp61F07012
fertile, with Klp61FUbi-p63E.GFP
fertile (with Klp61F06836), with Klp61FUbi-p63E.GFP
fertile (with Klp61F07012), with Klp61FUbi-p63E.GFP
Other Phenotypes
Allele
abnormal cytokinesis
abnormal mitotic cell cycle
abnormal mitotic cell cycle | embryonic stage 4 (with Klp61F3YF.Ubi-p63E.Tag:MYC)
abnormal mitotic cell cycle | embryonic stage 4 (with Klp61F07012)
abnormal mitotic cell cycle | larval stage
abnormal mitotic cell cycle | pre-blastoderm stage
abnormal mitotic cell cycle | pre-blastoderm stage (with Klp61F3YF.Ubi-p63E.Tag:MYC)
abnormal mitotic cell cycle | pre-blastoderm stage (with Klp61F07012)
abnormal mitotic cell cycle | recessive
abnormal mitotic cell cycle | somatic clone | third instar larval stage
abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4Bx-MS1096
abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4ptc.PU
abnormal neuroanatomy | larval stage, with Scer\GAL4insc-Mz1407
some die during embryonic stage | recessive
some die during larval stage (with Klp61F06836)
some die during larval stage (with Klp61F07012)
some die during larval stage | recessive
some die during pupal stage | recessive
some die during second instar larval stage | recessive
Phenotype manifest in
Allele
abdominal tergite 6 | female, with Scer\GAL4pnr-MD237
aster & neuroblast & larva
aster & spermatocyte
aster & spermatocyte (with Df(3L)bab-PG)
aster & spermatocyte (with Klp61F06345)
aster & spermatocyte (with Klp61F07012)
centriole & spermatocyte
centrosome, with Scer\GAL4ptc.PU
centrosome & neuroblast & larva
centrosome & spermatocyte
centrosome & spermatocyte (with Df(3L)bab-PG)
centrosome & spermatocyte (with Klp61F06345)
centrosome & spermatocyte (with Klp61F07012)
chromosome | oogenesis, with Scer\GAL4VP16.mat.αTub67C
dendritic branch | larval stage, with Scer\GAL4221
ganglion mother cell | larval stage, with Scer\GAL4insc-Mz1407
imaginal disc | larval stage
karyosome, with Scer\GAL4VP16.mat.αTub67C
larval brain
larval brain & nuclear chromosome & larva
larval brain | somatic clone | third instar larval stage
larval dorsal multidendritic neuron ddaE, with Scer\GAL4221
larval neuroblast
larval neuroblast | larval stage, with Scer\GAL4insc-Mz1407
larval neuroblast | somatic clone | third instar larval stage
meiotic spindle | oogenesis, with Scer\GAL4VP16.mat.αTub67C
meiotic telophase II & spermatocyte
microtubule organizing center | pre-blastoderm stage (with Klp61F3YF.Ubi-p63E.Tag:MYC)
microtubule organizing center | pre-blastoderm stage (with Klp61F07012)
microtubule plus-end | larval stage, with Scer\GAL4221
mitotic cell cycle & spermatocyte
mitotic spindle | third instar larval stage, with Scer\GAL4Bx-MS1096
mitotic spindle | third instar larval stage, with Scer\GAL4ptc.PU
nuclear chromosome
nuclear lamina & neuroblast & larva
nuclear lamina & spermatocyte
spermatid (with Klp61F07012)
spermatid (with Klp61Furc-1)
spermatocyte (with Klp61F07012)
spermatocyte (with Klp61Furc-1)
spermatocyte fusome
spindle
spindle & neuroblast & larva
spindle & spermatocyte
spindle & spermatocyte (with Df(3L)bab-PG)
spindle & spermatocyte (with Klp61F06345)
spindle & spermatocyte (with Klp61F07012)
spindle & spermatocyte (with Klp61Furc-1)
spindle | embryonic stage, with Klp61F06836/Klp61F07012
spindle | embryonic stage, with Klp61F07012
spindle | embryonic stage, with Klp61FUbi-p63E.GFP
spindle | embryonic stage (with Klp61F06836), with Klp61FUbi-p63E.GFP
spindle | embryonic stage (with Klp61F07012), with Klp61FUbi-p63E.GFP
spindle | pre-blastoderm stage
spindle microtubule | embryonic stage 4
spindle microtubule | embryonic stage 4 (with Klp61F3YF.Ubi-p63E.Tag:MYC)
spindle microtubule | embryonic stage 4 (with Klp61F07012)
testis
wing disc | third instar larval stage, with Scer\GAL4Bx-MS1096
wing disc | third instar larval stage, with Scer\GAL4ptc.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (42)
Hsap\KIF11
13 of 14
Yes
Yes
Hsap\CENPE
1 of 14
No
No
Hsap\GINS1
1 of 14
No
No
Hsap\KIF1A
1 of 14
No
No
Hsap\KIF1B
1 of 14
No
No
Hsap\KIF1C
1 of 14
No
No
Hsap\KIF2A
1 of 14
No
No
Hsap\KIF2B
1 of 14
No
No
Hsap\KIF2C
1 of 14
No
No
Hsap\KIF3A
1 of 14
No
No
1  
Hsap\KIF3B
1 of 14
No
No
Hsap\KIF3C
1 of 14
No
No
Hsap\KIF4A
1 of 14
No
No
Hsap\KIF4B
1 of 14
No
No
Hsap\KIF5A
1 of 14
No
No
5  
Hsap\KIF5B
1 of 14
No
No
1  
Hsap\KIF5C
1 of 14
No
No
Hsap\KIF6
1 of 14
No
No
Hsap\KIF7
1 of 14
No
No
1  
Hsap\KIF9
1 of 14
No
Yes
Hsap\KIF12
1 of 14
No
No
Hsap\KIF13A
1 of 14
No
No
Hsap\KIF13B
1 of 14
No
No
Hsap\KIF14
1 of 14
No
No
Hsap\KIF15
1 of 14
No
No
Hsap\KIF16B
1 of 14
No
No
Hsap\KIF17
1 of 14
No
No
Hsap\KIF18A
1 of 14
No
No
Hsap\KIF18B
1 of 14
No
No
Hsap\KIF19
1 of 14
No
No
Hsap\KIF20A
1 of 14
No
No
Hsap\KIF20B
1 of 14
No
No
Hsap\KIF21A
1 of 14
No
No
Hsap\KIF21B
1 of 14
No
No
1  
Hsap\KIF22
1 of 14
No
No
Hsap\KIF23
1 of 14
No
No
Hsap\KIF24
1 of 14
No
No
Hsap\KIF27
1 of 14
No
No
Hsap\KIF28P
1 of 14
No
Yes
Hsap\KIFC1
1 of 14
No
No
Hsap\KIFC2
1 of 14
No
No
Hsap\KIFC3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (33)
Rnor\Kif11
12 of 14
Yes
Yes
Rnor\Cenpe
1 of 14
No
No
Rnor\Gins1
1 of 14
No
No
Rnor\Kif1a
1 of 14
No
No
Rnor\Kif1b
1 of 14
No
No
Rnor\Kif1c
1 of 14
No
No
Rnor\Kif2a
1 of 14
No
No
Rnor\Kif2b
1 of 14
No
No
Rnor\Kif2c
1 of 14
No
No
Rnor\Kif3a
1 of 14
No
No
Rnor\Kif3b
1 of 14
No
No
Rnor\Kif3c
1 of 14
No
No
Rnor\Kif4a
1 of 14
No
No
Rnor\Kif5a
1 of 14
No
No
Rnor\Kif5b
1 of 14
No
No
Rnor\Kif7
1 of 14
No
No
Rnor\Kif12
1 of 14
No
No
Rnor\Kif13b
1 of 14
No
No
Rnor\Kif14
1 of 14
No
No
Rnor\Kif15
1 of 14
No
No
Rnor\Kif16b
1 of 14
No
No
Rnor\Kif17
1 of 14
No
No
Rnor\Kif18b
1 of 14
No
No
Rnor\Kif19
1 of 14
No
No
Rnor\Kif20a
1 of 14
No
No
Rnor\Kif20b
1 of 14
No
No
Rnor\Kif21a
1 of 14
No
No
Rnor\Kif21b
1 of 14
No
No
Rnor\Kif22
1 of 14
No
No
Rnor\Kif23
1 of 14
No
No
Rnor\Kif24
1 of 14
No
No
Rnor\Kif27
1 of 14
No
No
Rnor\Stard9
1 of 14
No
No
Mus musculus (laboratory mouse) (34)
Mmus\Kif11
12 of 14
Yes
Yes
Mmus\Cenpe
1 of 14
No
No
Mmus\Gins1
1 of 14
No
No
Mmus\Kif1a
1 of 14
No
No
Mmus\Kif1c
1 of 14
No
No
Mmus\Kif2a
1 of 14
No
No
Mmus\Kif2b
1 of 14
No
No
Mmus\Kif2c
1 of 14
No
No
Mmus\Kif3a
1 of 14
No
No
1  
Mmus\Kif3b
1 of 14
No
No
Mmus\Kif3c
1 of 14
No
No
Mmus\Kif4
1 of 14
No
No
Mmus\Kif5a
1 of 14
No
No
Mmus\Kif5b
1 of 14
No
No
Mmus\Kif6
1 of 14
No
No
Mmus\Kif7
1 of 14
No
No
Mmus\Kif13a
1 of 14
No
No
Mmus\Kif13b
1 of 14
No
No
Mmus\Kif14
1 of 14
No
No
Mmus\Kif15
1 of 14
No
No
Mmus\Kif16b
1 of 14
No
No
Mmus\Kif17
1 of 14
No
No
Mmus\Kif18a
1 of 14
No
No
Mmus\Kif18b
1 of 14
No
No
Mmus\Kif19a
1 of 14
No
No
Mmus\Kif19b
1 of 14
No
No
Mmus\Kif20a
1 of 14
No
No
Mmus\Kif20b
1 of 14
No
No
Mmus\Kif21a
1 of 14
No
No
1  
Mmus\Kif21b
1 of 14
No
No
Mmus\Kif22
1 of 14
No
No
Mmus\Kif23
1 of 14
No
No
Mmus\Kif24
1 of 14
No
No
Mmus\Kif27
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (20)
Xtro\kif11
10 of 13
Yes
Yes
Xtro\dcun1d5
1 of 13
No
No
Xtro\gins1
1 of 13
No
No
Xtro\kif5b
1 of 13
No
No
Xtro\kif5c
1 of 13
No
No
Xtro\kif9
1 of 13
No
No
Xtro\kif13a
1 of 13
No
No
Xtro\kif13b
1 of 13
No
No
Xtro\kif14
1 of 13
No
No
Xtro\kif18a
1 of 13
No
No
Xtro\kif18b
1 of 13
No
No
Xtro\kif20a
1 of 13
No
No
Xtro\kif20b
1 of 13
No
No
Xtro\kif22
1 of 13
No
No
Xtro\kif23
1 of 13
No
No
Xtro\kif27
1 of 13
No
No
Xtro\kifc1
1 of 13
No
No
Xtro\kifc2
1 of 13
No
No
Xtro\LOC100490157
1 of 13
No
No
Xtro\mycbpap
1 of 13
No
Yes
Danio rerio (Zebrafish) (32)
Drer\kif11
13 of 14
Yes
Yes
Drer\cenpe
1 of 14
No
No
Drer\gins1
1 of 14
No
No
Drer\kif1aa
1 of 14
No
No
Drer\kif1ab
1 of 14
No
No
Drer\kif1b
1 of 14
No
No
Drer\kif2c
1 of 14
No
No
Drer\kif3a
1 of 14
No
No
Drer\kif3b
1 of 14
No
No
Drer\kif3cb
1 of 14
No
No
Drer\kif3c
1 of 14
No
No
Drer\kif4
1 of 14
No
No
Drer\kif5aa
1 of 14
No
No
Drer\kif5ba
1 of 14
No
No
Drer\kif7
1 of 14
No
No
Drer\kif13ba
1 of 14
No
No
Drer\kif13bb
1 of 14
No
No
Drer\kif14
1 of 14
No
No
Drer\kif15
1 of 14
No
No
Drer\kif17
1 of 14
No
No
Drer\kif18a
1 of 14
No
No
Drer\kif19
1 of 14
No
No
Drer\kif20a
1 of 14
No
No
Drer\kif20bb
1 of 14
No
No
Drer\kif21a
1 of 14
No
No
Drer\kif21b
1 of 14
No
No
Drer\kif22
1 of 14
No
No
Drer\kif23
1 of 14
No
No
Drer\si:ch211-63b16.4
1 of 14
No
Yes
Drer\si:dkey-26i13.8
1 of 14
No
No
Drer\zgc:56231
1 of 14
No
No
Drer\zgc:103670
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (19)
Cele\bmk-1
11 of 14
Yes
Yes
Cele\klp-3
1 of 14
No
No
Cele\klp-4
1 of 14
No
No
Cele\klp-6
1 of 14
No
No
Cele\klp-7
1 of 14
No
No
Cele\klp-11
1 of 14
No
No
Cele\klp-12
1 of 14
No
No
Cele\klp-13
1 of 14
No
No
Cele\klp-15
1 of 14
No
No
Cele\klp-16
1 of 14
No
No
Cele\klp-17
1 of 14
No
No
Cele\klp-18
1 of 14
No
No
Cele\klp-19
1 of 14
No
No
Cele\klp-20
1 of 14
No
No
Cele\osm-3
1 of 14
No
No
Cele\psf-1
1 of 14
No
No
Cele\unc-104
1 of 14
No
No
Cele\unc-116
1 of 14
No
No
Cele\zen-4
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (20)
Agam\AgaP_AGAP007502
10 of 12
Yes
Yes
Agam\AgaP_AGAP000561
2 of 12
No
No
Agam\AgaP_AGAP002427
2 of 12
No
No
Agam\AgaP_AGAP003323
2 of 12
No
No
Agam\AgaP_AGAP003925
2 of 12
No
No
Agam\AgaP_AGAP010396
2 of 12
No
No
Agam\AgaP_AGAP000159
1 of 12
No
No
Agam\AgaP_AGAP000575
1 of 12
No
No
Agam\AgaP_AGAP002248
1 of 12
No
No
Agam\AgaP_AGAP006472
1 of 12
No
No
Agam\AgaP_AGAP006803
1 of 12
No
No
Agam\AgaP_AGAP006989
1 of 12
No
No
Agam\AgaP_AGAP007592
1 of 12
No
No
Agam\AgaP_AGAP007706
1 of 12
No
No
Agam\AgaP_AGAP007815
1 of 12
No
No
Agam\AgaP_AGAP008035
1 of 12
No
No
Agam\AgaP_AGAP010835
1 of 12
No
No
Agam\AgaP_AGAP011874
1 of 12
No
No
Agam\AgaP_AGAP012471
1 of 12
No
No
Agam\KIF1A_ANOGA
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (61)
Atha\AT2G28620
11 of 13
Yes
Yes
Atha\AT2G36200
11 of 13
Yes
Yes
Atha\AT2G37420
11 of 13
Yes
Yes
Atha\AT3G45850
11 of 13
Yes
Yes
Atha\AT4G14330
2 of 13
No
Yes
Atha\ARK2
1 of 13
No
No
Atha\ARK3
1 of 13
No
No
Atha\AT1G09170
1 of 13
No
No
Atha\AT1G18410
1 of 13
No
No
Atha\AT1G18550
1 of 13
No
No
Atha\AT1G20060
1 of 13
No
No
Atha\AT1G21730
1 of 13
No
No
Atha\AT1G55550
1 of 13
No
No
Atha\AT1G63640
1 of 13
No
No
Atha\AT1G72250
1 of 13
No
No
Atha\AT1G73860
1 of 13
No
No
Atha\AT2G21300
1 of 13
No
No
Atha\AT2G21380
1 of 13
No
No
Atha\AT2G22610
1 of 13
No
No
Atha\AT2G47500
1 of 13
No
No
Atha\AT3G10180
1 of 13
No
No
Atha\AT3G10310
1 of 13
No
No
Atha\AT3G12020
1 of 13
No
No
Atha\AT3G16060
1 of 13
No
No
Atha\AT3G20150
1 of 13
No
No
Atha\AT3G44050
1 of 13
No
No
Atha\AT3G49650
1 of 13
No
No
Atha\AT3G51150
1 of 13
No
No
Atha\AT3G63480
1 of 13
No
No
Atha\AT4G24170
1 of 13
No
No
Atha\AT4G26660
1 of 13
No
Yes
Atha\AT4G38950
1 of 13
No
No
Atha\AT4G39050
1 of 13
No
No
Atha\AT5G02370
1 of 13
No
No
Atha\AT5G06670
1 of 13
No
No
Atha\AT5G23910
1 of 13
No
Yes
Atha\AT5G27550
1 of 13
No
No
Atha\AT5G27950
1 of 13
No
No
Atha\AT5G41310
1 of 13
No
No
Atha\AT5G55520
1 of 13
No
Yes
Atha\AT5G60930
1 of 13
No
No
Atha\AT5G66310
1 of 13
No
No
Atha\ATK1
1 of 13
No
No
Atha\ATK2
1 of 13
No
No
Atha\ATK3
1 of 13
No
No
Atha\ATK4
1 of 13
No
No
Atha\ATK5
1 of 13
No
No
Atha\FRA1
1 of 13
No
No
Atha\HIK
1 of 13
No
No
Atha\KAC2
1 of 13
No
No
Atha\KICP-02
1 of 13
No
No
Atha\KINESIN-12B
1 of 13
No
No
Atha\KINESIN-13A
1 of 13
No
No
Atha\KP1
1 of 13
No
No
Atha\MRH2
1 of 13
No
No
Atha\PAKRP1
1 of 13
No
No
Atha\POK1
1 of 13
No
No
Atha\POK2
1 of 13
No
No
Atha\TES
1 of 13
No
No
Atha\ZCF125
1 of 13
No
No
Atha\ZWI
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\KIP1
9 of 13
Yes
Yes
Scer\CIN8
4 of 13
No
Yes
Scer\KAR3
1 of 13
No
No
Scer\KIP2
1 of 13
No
No
Scer\KIP3
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\cut7
10 of 12
Yes
Yes
Spom\klp3
1 of 12
No
No
Spom\klp5
1 of 12
No
No
Spom\klp6
1 of 12
No
No
Spom\klp9
1 of 12
No
No
Spom\pkl1
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Klp61F. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (26)
CG32318
3 of 13
Khc
3 of 13
Kif3C
3 of 13
Klp64D
3 of 13
Klp68D
3 of 13
Klp98A
3 of 13
cmet
2 of 13
Khc-73
2 of 13
Kif19A
2 of 13
Klp3A
2 of 13
Klp10A
2 of 13
Klp31E
2 of 13
Klp54D
2 of 13
Klp59C
2 of 13
Klp59D
2 of 13
Klp67A
2 of 13
ncd
2 of 13
nod
2 of 13
pav
2 of 13
sub
2 of 13
unc-104
2 of 13
cana
1 of 13
CG5004
1 of 13
cos
1 of 13
neb
1 of 13
Psf1
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 4 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KIF11; kinesin family member 11
    13 of 14
    148760
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    esyN Network Key:
    Suppression
    Enhancement
    Other Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Klp61F
    suppressible
    asp
    (Radford et al., 2017)
    Klp61F
    suppressible
    Klp54D
    (Radford et al., 2017)
    Klp61F
    suppressible
    ncd
    (Radford et al., 2017, Wilson et al., 2004)
    Klp61F
    enhanceable
    ncd
    (Wilson et al., 2004)
    Klp61F
    suppressible
    Spc105R
    (Radford et al., 2017)
    Klp61F
    enhanceable
    sub
    (Radford et al., 2017)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    tau
    enhanceable
    Klp61F
    (Bougé and Parmentier, 2016)
    External Data
    Subunit Structure (UniProtKB)
    Homotetramer (PubMed:24714498, PubMed:8538794, PubMed:9885249). Consists of two pairs of polypeptides associated by coiled-coil interactions to form two homodimers (PubMed:8538794). The homodimers are linked by lateral interactions between their coiled-coil regions to form a bipolar homotetramer consisting of a central rod with two motor domains projecting from either end (PubMed:8538794). Parallel coiled coils extend from each pair of motor heads, switch to two antiparallel coiled coils in the central region and then back to parallel coiled coils (PubMed:24714498). Interacts with Wee1 (PubMed:19800237).
    (UniProt, P46863 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-0
    Cytogenetic map
    61F4-61F4
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    61F4-61F4
    Limits computationally determined from genome sequence between P{PZ}l(3)0596705967 and P{PZ}l(3)0264002640
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    61F3-61F4
    (determined by in situ hybridisation)
    (Spradling et al., 1999, Heck et al., 1993)
    61F-61F
    (determined by in situ hybridisation)
    (Omel'yanchuk et al., 1997)
    61F1-61F8
    61F5--6
    (BDGP Project Members, 1994-1999)
    Experimentally Determined Recombination Data
    Location

    3-0

    (FlyBase, 2016)

    3-1.5

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (16)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (195)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Hwang et al., 2020, Brust-Mascher et al., 2009, Sharp et al., 1999, Barton et al., 1995)
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

        (Goshima et al., 2007)

        dsRNA directed against Klp61F inhibits protein secretion in S2 cells and causes aggregation and swelling of the Golgi membranes in more than 50% of the treated cells.

        (Bard et al., 2006)

        dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.

        (Bard et al., 2006)

        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: during mitosis, centrosome separation is prevented, resulting in monopolar spindles.

        (Gandhi et al., 2004)

        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

        (Kiger et al., 2003)

        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.

        (Kiger et al., 2003)

        Spindle pole movements in embryos are directed by a temporally coordinated balance of forces generated by three mitotic motors; cytoplasmic dynein, Klp61F and ncd. Dynein acts to move the poles apart throughout mitosis, and this activity is augmented by Klp61F after the fenestration of the nuclear envelope, which occurs at the onset of prometaphase. ncd generates forces that pull the poles together between interphase and metaphase, antagonising the activity of both cytoplasmic dynein and Klp61F and serving as a brake for spindle assembly.

        (Sharp et al., 2000)

        Klp61F cycles between spindles during mitosis and fusomes during interphase.

        (Wilson, 1999)

        Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

        (Harvie et al., 1998)

        Klp61F is involved in the control of cell division.

        (Omel'yanchuk et al., 1997)

        Klp61F protein, a homotetrameric BimC-related kinesin, consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends. Such a bipolar 'minifilament' could crosslink spindle microtubules and slide them relative to one another.

        (Kashina et al., 1996)

        Antibody staining reveals multiple bimC family members are expressed in Drosophila. Klp61F is an essential gene and biochemical and functional analysis of the protein demonstrates it is not functionally redundant for centrosome separation during neuroblast separation.

        (Barton et al., 1995)

        Relationship to cloned Klp genes not known. Purified Klp61F behaves as a homotetrameric complex of four subunits that moves toward the plus ends of microtubule tracts at approximately 0.04μm/s.

        (Cole and Scholey, 1995)

        Encodes a 130kD kinesin related protein that behaves as a homotetramer and moves towards the plus ends of microtubules.

        (Cole and Scholey, 1995)

        Identified as a 130kD polypeptide in an ATP MAPs 1-24 hour embryonic fraction. A 490kD homotetrameric complex consisting of 4 130kD subunits display a 'slow' plus-end directed motor activity capable of moving single microtubules.

        (Cole et al., 1994)

        The sequence of the Klp61F protein has been compared with the sequences of a variety of kinesin family proteins.

        (Goodson et al., 1994)

        Klp61F has been cloned, partially sequenced and its expression pattern analysed.

        (Stewart et al., 1991)
        Relationship to Other Genes
        Source for database merge of

        Source for merge of: Klp61F Krp130

        (Scholey, 1999.5.5)
        Additional comments

        CG32318 appears to be a chimeric gene, with the 5' part of the gene derived from Klp61F and the 3' part of the gene derived from Psf1.

        (Rogers and Hartl, 2012)

        "CG32318" is a putative chimeric gene derived from the "Klp61F" and "Psf1" genes (where coding sequences of the two parental genes contribute to the coding sequence of the chimeric gene).

        (Rogers et al., 2009)
        Nomenclature History
        Source for database identify of
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (40)
        Reported As
        Symbol Synonym
        6345
        (Harvie et al., 1998)
        CG9191
        (Kolkhof et al., 2017, Çiçek et al., 2016, Salemi et al., 2013, Rogers and Hartl, 2012, Ni et al., 2011.7.19, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ducat et al., 2008, Hughes et al., 2008, Goshima et al., 2007, Goldstein and Gunawardena, 2000)
        CG9191-PA
        (Rogers et al., 2009)
        DmKLP61F
        (Gadde and Heald, 2004, Matthies et al., 2001, Hirokawa et al., 1998)
        DmKRP130
        (Moore and Endow, 1996)
        DmKlp61F
        (Hirokawa, 2012, Dagenbach and Endow, 2004, Kim and Endow, 2000, Moore and Endow, 1996)
        KIF 8
        (Miki et al., 2001)
        KIF11
        (Huber et al., 2023, Hirokawa, 2012.11.20)
        KLP2
        (Wilson et al., 1992, Stewart et al., 1991)
        KLP61
        (Gonzalez et al., 1998)
        KLP61F
        (Nithianantham et al., 2023, Wang et al., 2015, Scholey et al., 2014, Civelekoglu-Scholey et al., 2010, Sommi et al., 2010, Tao and Scholey, 2010, Bodey et al., 2009, Brust-Mascher et al., 2009, Cheerambathur et al., 2008, van den Wildenberg et al., 2008, Vendra et al., 2007, Silverman-Gavrila and Wilde, 2006, Tao et al., 2006, Wilson, 2005, Wilson et al., 2005, Kwon and Scholey, 2004, Lawrence et al., 2002, Adams et al., 2001, Wilson, 1999, Wilson, 1999, Compton, 1998, Glover et al., 1998, Kashina et al., 1997, Kashina et al., 1996, Wilson and Borisy, 1996, Barton et al., 1994, Heck et al., 1993)
        KLP61f
        (Kai et al., 2005)
        KRP130
        (Kashina et al., 1997)
        KRP(130)
        (Cole and Scholey, 1995)
        KRP130
        (Kashina et al., 1996, Cole and Scholey, 1995, Kashina et al., 1995, Cole et al., 1994, Cole et al., 1994)
        Kin-5
        (Lv et al., 2021, Hesse et al., 2013)
        Kin5
        (Lv et al., 2020)
        Klp 61F
        (Miki et al., 2001)
        Klp2
        Klp61F
        (Khalili et al., 2023, Adashev et al., 2022, Beaver et al., 2022, Deshpande et al., 2022, Hodgson et al., 2022, Rolls, 2022, Cattenoz et al., 2021, Deng et al., 2021, Evans et al., 2021, Feng et al., 2021, Ota et al., 2021, Singh et al., 2020, Witte et al., 2020, Yusuff et al., 2020, Blake-Hedges and Megraw, 2019, Costa and Ohkura, 2019, Hughes et al., 2018, Lv et al., 2018, Tubman et al., 2018, Radford et al., 2017, Bougé and Parmentier, 2016, Iyer et al., 2016, Ye et al., 2016, Dembeck et al., 2015, Appocher et al., 2014, Vuong et al., 2014, Cane et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Salemi et al., 2013, Syred et al., 2013, Wakana et al., 2013, Hirokawa, 2012.11.20, Japanese National Institute of Genetics, 2012.5.21, Kalajdzic et al., 2012, Ambegaokar and Jackson, 2011, Lindquist et al., 2011, Liu et al., 2011, Neumüller et al., 2011, Anholt and Williams, 2010, Goldstein, 2010.9.10, Lakämper et al., 2010, Müller et al., 2010, Christensen et al., 2009.5.6, Rogers et al., 2009, Vale et al., 2009, Christensen et al., 2008.4.15, Ducat et al., 2008, Fisher et al., 2008, Somma et al., 2008, Christensen et al., 2007.10.29, Christensen et al., 2007.10.29, Georlette et al., 2007, Goshima et al., 2007, Kim et al., 2007, Slack et al., 2006, De Lucia et al., 2005, Goshima and Vale, 2005, Miki et al., 2005, Loop et al., 2004, Wei et al., 2003)
        Klp61F/KRP130
        (Endow, 1999)
        Klp61f
        (Yang, 2021, Noble et al., 2016, Casper and Van Doren, 2009)
        Krp130
        klp61F
        (Pendse et al., 2013, Mottier-Pavie et al., 2011, Garcia et al., 2009, Gaspar and Szabad, 2009)
        klp61f
        (Joyce et al., 2012, Cheerambathur et al., 2008, Wilson et al., 2004, Wilson et al., 2004.8.21)
        l(3)07012
        l(3)6D283
        (Wei et al., 2003)
        l(3)Klp61F
        (Wei et al., 2003)
        urc
        urcn
        (Wilson and Borisy, 1995, Wilson and Fuller, 1993)
        Name Synonyms
        Kinesin-5
        (Lv et al., 2018)
        Kinesin-like protein at 61F
        (Hirokawa, 2012.11.20)
        Kinesin-related protein-130kD
        (Cole and Scholey, 1995)
        Kinesin8B
        (Kollmar, 2015.9.15)
        kinesin-5
        (Ye et al., 2016, Acar et al., 2013, Hesse et al., 2013, Civelekoglu-Scholey et al., 2010, Tao and Scholey, 2010, Garcia et al., 2009, Cheerambathur et al., 2008)
        kinesin-5 motor
        (Vale et al., 2009)
        kinesin-5s
        (Salemi et al., 2013)
        kinesin-like protein
        (Goldstein, 1993.9.15)
        kinesin-like protein at 61F
        (Taniguchi et al., 2014)
        urchin
        (Wilson and Borisy, 1995, Goodson et al., 1994, Wilson, 1994, Wilson et al., 1992, Wilson and Fuller, 1991)
        Secondary FlyBase IDs
        • FBgn0004923
        • FBgn0010911
        • FBgn0015744
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 69 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        PDB - An information portal to biological macromolecular structures
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        References (233)