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General Information
Symbol
Dmel\Klp61F
Species
D. melanogaster
Name
Kinesin-like protein at 61F
Annotation Symbol
CG9191
Feature Type
FlyBase ID
FBgn0004378
Gene Model Status
Stock Availability
Enzyme Name (EC)
Adenosinetriphosphatase (3.6.1.3)
Gene Snapshot
Kinesin-like protein at 61F (Klp61F) encodes a member of the kinesin-5 family of cytoskeletal motor proteins. It allows the crosslinking and sliding apart of adjacent microtubules. This 'sliding filament mechanism' is critical for many aspect of mitosis and chromosome segregation. [Date last reviewed: 2019-03-14]
Also Known As
kinesin-5, urchin, KRP130, DmKlp61F, KLP2
Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:1,241,568..1,245,910 [-]
Recombination map
3-0
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily. (P46863)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O = ADP + phosphate (3.6.1.3)
Summaries
Gene Group (FlyBase)
KINESINS -
Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).
Protein Function (UniProtKB)
Important role in mitotic dividing cells (PubMed:8227131). Microtubule motor required for spindle body separation (PubMed:8918872). Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function (PubMed:8918872, PubMed:8589456, PubMed:19062285). Forms cross-links between microtubules within interpolar microtubule bundles (PubMed:9885249, PubMed:19158379). Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates (PubMed:19158379). Displays microtubule-stimulated ATPase activity (PubMed:8589456). Required for normal fusome organization (PubMed:10469596). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
(UniProt, P46863)
Summary (Interactive Fly)
motor protein - crosslinking and sliding activities during mitosis facilitate accumulation within antiparallel interpolar MTs at the equator, where the motor generates force to drive spindle poleward flux and pole-pole separation
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Klp61F or the JBrowse view of Dmel\Klp61F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.46
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0072733
3871
1066
Additional Transcript Data and Comments
Reported size (kB)
3.7 (longest cDNA)
3.9, 3.7 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0072616
121.2
1066
6.39
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
120 (kD observed)
130 (kD observed)
Comments
External Data
Subunit Structure (UniProtKB)
Homotetramer (PubMed:8538794, PubMed:9885249, PubMed:24714498). Consists of two pairs of polypeptides associated by coiled-coil interactions to form two homodimers (PubMed:8538794). The homodimers are linked by lateral interactions between their coiled-coil regions to form a bipolar homotetramer consisting of a central rod with two motor domains projecting from either end (PubMed:8538794). Parallel coiled coils extend from each pair of motor heads, switch to two antiparallel coiled coils in the central region and then back to parallel coiled coils (PubMed:24714498). Interacts with Wee1 (PubMed:19800237).
(UniProt, P46863)
Post Translational Modification
Phosphorylation is required for localization to mitotic spindles (PubMed:9885249). Phosphorylation of Thr-933 during mitosis controls association with the spindle apparatus (By similarity). Phosphorylated in vitro by Wee1 (PubMed:19800237).
(UniProt, P46863)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp61F using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (34 terms)
Molecular Function (7 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from physical interaction with UniProtKB:P54350
(assigned by UniProt )
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000649234
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
Biological Process (12 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:ncd; FB:FBgn0002924
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000649235
(assigned by GO_Central )
Cellular Component (15 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
colocalizes_with centrosome
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000649235
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000649235
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Klp61F transcripts were detected throughout the cytoplasm of unfertilized eggs. The maternal RNA persists until cellularization. During gastrulation, Klp61F transcripts are first detected in a wide band of ventral cells corresponding to the invaginating presumptive mesoderm. They are also seen in a more dorsal strip of cells and underneath the pole cells. Later embryos show expression in the head, along the cephalic furrow, along the ventral midline, and within the posterior region. After germ band retraction, expression is limited to the cells of the head and ventral neurogenic region. Regions of expression are well correlated with domains of cell division.
Klp61F transcripts are expressed throughout development. In adults, they are detected only in the gonads.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Throughout mitosis, nonphosphorylated Klp61F protein is diffuse and cytoplasmic. Phosphorylated Klp61F shows a very different distribution. During interphase and prophase, the staining is punctate and concentrated in the nucleus. Occasional faint staining is seen in the centrosome in prophase. By metaphase, the staining localizes to the spindle and appears filamentous. Phospho-Klp61F is particularly concentrated on the half spindles and densely stained bridges between the spindles in the midzone. Lower level staining occurs at the oles and grainy cytoplasmic staining is observed. During anaphase A, the staining resembles metaphase. During anaphase B, nearly all the phospho-Klp61F is present in the spindle between the separating nuclei. Filaments of phospho-Klp61F protein staining cross the midzone while little or no staining is associated with the spindle poles and astral microtubules. During telophase, Klp61F staining is most pronounced on two adjacent patches on the remnants of the central spindle. EM studies suggest that the phospho-Klp61F motors form crosslinks between microtubules within interpolar microt bule bundles.
Klp61F protein localizes to the structural domains of the mitotic spindle throughout mitosis.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
colocalizes_with centrosome
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Klp61F in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Klp61F
Transgenic constructs containing regulatory region of Klp61F
Deletions and Duplications ( 9 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
aster & neuroblast & larva
aster & spermatocyte
aster & spermatocyte (with Df(3L)bab-PG)
aster & spermatocyte (with Klp61F06345)
aster & spermatocyte (with Klp61F07012)
centriole & spermatocyte
centrosome & neuroblast & larva
centrosome & spermatocyte
centrosome & spermatocyte (with Df(3L)bab-PG)
centrosome & spermatocyte (with Klp61F06345)
centrosome & spermatocyte (with Klp61F07012)
larval brain & nuclear chromosome & larva
meiotic telophase II & spermatocyte
mitotic cell cycle & spermatocyte
nuclear lamina & neuroblast & larva
nuclear lamina & spermatocyte
spindle & neuroblast & larva
spindle & spermatocyte
spindle & spermatocyte (with Df(3L)bab-PG)
spindle & spermatocyte (with Klp61F06345)
spindle & spermatocyte (with Klp61F07012)
spindle & spermatocyte (with Klp61Furc-1)
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (17)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
2 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (16)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
2 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (17)
7 of 13
Yes
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (21)
9 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (18)
13 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
12 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (5)
7 of 9
Yes
Yes
7 of 9
Yes
Yes
7 of 9
Yes
Yes
5 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (4)
13 of 15
Yes
Yes
8 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (5)
10 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901QY )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915024C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03JT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03GJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G013C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (25)
4 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 3 )
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homotetramer (PubMed:8538794, PubMed:9885249, PubMed:24714498). Consists of two pairs of polypeptides associated by coiled-coil interactions to form two homodimers (PubMed:8538794). The homodimers are linked by lateral interactions between their coiled-coil regions to form a bipolar homotetramer consisting of a central rod with two motor domains projecting from either end (PubMed:8538794). Parallel coiled coils extend from each pair of motor heads, switch to two antiparallel coiled coils in the central region and then back to parallel coiled coils (PubMed:24714498). Interacts with Wee1 (PubMed:19800237).
    (UniProt, P46863 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-0
    Cytogenetic map
    Sequence location
    3L:1,241,568..1,245,910 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    61F4-61F4
    Limits computationally determined from genome sequence between P{PZ}l(3)0596705967 and P{PZ}l(3)0264002640
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    61F-61F
    (determined by in situ hybridisation)
    61F3-61F4
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (15)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (197)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Source for merge of: Klp61F Krp130
    Additional comments
    CG32318 appears to be a chimeric gene, with the 5' part of the gene derived from Klp61F and the 3' part of the gene derived from Psf1.
    "CG32318" is a putative chimeric gene derived from the "Klp61F" and "Psf1" genes (where coding sequences of the two parental genes contribute to the coding sequence of the chimeric gene).
    Other Comments
    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
    dsRNA directed against Klp61F inhibits protein secretion in S2 cells and causes aggregation and swelling of the Golgi membranes in more than 50% of the treated cells.
    dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.
    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: during mitosis, centrosome separation is prevented, resulting in monopolar spindles.
    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.
    Spindle pole movements in embryos are directed by a temporally coordinated balance of forces generated by three mitotic motors; cytoplasmic dynein, Klp61F and ncd. Dynein acts to move the poles apart throughout mitosis, and this activity is augmented by Klp61F after the fenestration of the nuclear envelope, which occurs at the onset of prometaphase. ncd generates forces that pull the poles together between interphase and metaphase, antagonising the activity of both cytoplasmic dynein and Klp61F and serving as a brake for spindle assembly.
    Klp61F cycles between spindles during mitosis and fusomes during interphase.
    Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
    Klp61F is involved in the control of cell division.
    Klp61F protein, a homotetrameric BimC-related kinesin, consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends. Such a bipolar 'minifilament' could crosslink spindle microtubules and slide them relative to one another.
    Antibody staining reveals multiple bimC family members are expressed in Drosophila. Klp61F is an essential gene and biochemical and functional analysis of the protein demonstrates it is not functionally redundant for centrosome separation during neuroblast separation.
    Relationship to cloned Klp genes not known. Purified Klp61F behaves as a homotetrameric complex of four subunits that moves toward the plus ends of microtubule tracts at approximately 0.04μm/s.
    Encodes a 130kD kinesin related protein that behaves as a homotetramer and moves towards the plus ends of microtubules.
    Identified as a 130kD polypeptide in an ATP MAPs 1-24 hour embryonic fraction. A 490kD homotetrameric complex consisting of 4 130kD subunits display a 'slow' plus-end directed motor activity capable of moving single microtubules.
    The sequence of the Klp61F protein has been compared with the sequences of a variety of kinesin family proteins.
    Klp61F has been cloned, partially sequenced and its expression pattern analysed.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 51 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (38)
    Reported As
    Symbol Synonym
    Klp2
    Klp61F/KRP130
    Krp130
    l(3)07012
    l(3)6D283
    l(3)Klp61F
    urc
    Secondary FlyBase IDs
    • FBgn0004923
    • FBgn0010911
    • FBgn0015744
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
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    References (201)