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Gene Dmel\Klp61F

General Information
Symbol	Dmel\Klp61F	Species	D. melanogaster
Name	Kinesin-like protein at 61F	Annotation symbol	CG9191
Feature type	protein_coding_gene	FlyBase ID	FBgn0004378
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	DmKLP61F, KRP130, urcn, KLP2
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	61F4-61F4	Sequence location	3L:1,241,568..1,245,935 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Kinesin-like protein at 61F is referred to in FlyBase by the symbol Dmel\Klp61F (CG9191, FBgn0004378). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: motor activity. There is experimental evidence for 13 unique biological process terms, many of which group under: cellular component organization or biogenesis; cell cycle; cell cycle process; organelle organization; cellular component movement; localization; biological regulation; anatomical structure development; microtubule bundle formation; secretion by cell; protein complex biogenesis; microtubule-based movement; multicellular organismal process. 38 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; organelle; anatomical structure; spermatocyte; somatic cell; protein complex; spindle; nuclear part; cytoplasmic part; somatic stem cell; organ system subdivision. It has one annotated transcript and one annotated polypeptide. Protein features are: Kinesin, motor domain; Kinesin, motor region, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1624620_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Klp61F. Gene sequence location is 3L:1241568..1245935.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Lawrence et al., 2002, J. molec. Evol. 54(1): 42--53 Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	Controlled Vocabulary Terms centrosome duplication Sequence features BKN45948 HFA08671 BKN20600 Alleles Klp61F[DL42] Klp61F[B14] Klp61F[H10] Klp61F[A9] Klp61F[A67] References Müller et al., 2010, EMBO J. 29(19): 3344--3357
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 61F4-61F4   Limits computationally determined from genome sequence between P{PZ}l(3)0596705967 and P{PZ}l(3)0264002640
Experimentally Determined Cytological Location
Cytogenetic map Notes References 61F3-61F4   (determined by in situ hybridisation)   (Spradling et al., 1999) 61F-61F   (determined by in situ hybridisation)   (Omel'yanchuk et al., 1997) 61F1-61F8   61F5--6   (BDGP Project Members, 1994-1999) 61F3-61F4   (determined by in situ hybridisation)   (Heck et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Klp61F for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Klp61F-RA FBtr0072733  NM_057470   3896   1066
Additional Transcript Data & Comments
Reported size (kB)	3.9, 3.7 (northern blot) (Stewart et al., 1991) 3.7 (longest cDNA) (Heck et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Klp61F-PA   FBpp0072616   121.2   1066   6.39     NP_476818   AAF47458
Additional Polypeptide Data & Comments
Reported size (kDa)	1066 (aa) (Heck et al., 1993) 130 (kD observed) (Cole et al., 1994) 120 (kD observed) (Barton et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Kinesin, motor domain (IPR001752) Kinesin, motor region, conserved site (IPR019821)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201190   AA263815   AA391627   AA391713   AA391978   AA392036   AA438651   AA438977   AA439890   AA440767   AA538841   AA539314   AA539786   AA735699   AA801784   AA803527   AA803957   AA817061   AA817215   AA940618   AA940782   AA941605   AA941872   AA950728   AA950760   AA951257   AA979456   AA990827   AE014296 AAF47458   AC004489   AC005847   AI455672   AI456846   AI511793   AI512850   AI513910   AI515955   AI518651   AI520386   AI530932   AI532042   AI532659   AI542240   AQ026225   AW942371   AY069442 AAL39587   AY729990   BF491061   BF502702   BG635989   BI234322   BI235593   BI354371   BI367250   BI578223   BI638360   BT124785   CO177325   CO266434   CO274192   CO293307   CO299347   CO303911   CO303914   CO304278   CO308414   CO315257   CO318956   CO319606   CO325072   CO328333   CO329944   CZ485426   DQ782381 ABG91086   DQ782382 ABG91087   EC076596   EC083499   EC084477   EC085824   EC086374   EC201850   EC203029   EC207150   EC215784   EC215785   EC224228   EC250751   EC251891   EC258823   EC265606   EV580197   GH747759   GH747803   GH747815   GH747839   GH748435   GH748455   GH748661   GH753437   GH753811   GH754713   GH754928   GH755246   GH757440   GH761358   GH771009   GH771179   GH771592   GH772031   GH772900   GH773873   GH774095   GH776507   GH777041   GH777433   GH778696   GH781212   GH783257   GH783262   GH784838   GH785140   GH787060   GH788023   GH789781   GH789920   GH790967   GH792145   GH793188   GH794797   GH795890   GH802442   GH805004   GH805590   GH805615   GH806021   GH809352   GH810380   GH810923   GH811079   GH814948   GH816251   GH822265   GH823502   GH825642   GH826321   GH829840   GH829887   GH830176   GH830317   GH830514   GH831288   GH831553   GH835778   GH838160   GH838418   GH839984   GH840307   GH840901   GH841064   GH842530   GH842654   GH843105   GH844871   GH845111   GH845575   GH846368   GH847352   GH852865   GH857291   GH857565   GH859461   GH862613   GH863441   GH865006   GH865987   GH867576   GH869853   GH870033   GH870661   GH870818   GH871875   GH875640   GH878013   GH878803   GH880389   GH881020   GH882612   GH883954   GH884893   GH886404   GH889767   GH890961   GH892995   GH893841   GH899862   GH903288   GH903653   GH906191   GH907535   GH909714   GH910633   GH911682   GH912019   GH914978   GH915194   GH916457   GH916589   GH917181   GH917188   GH917251   GH917461   GH917912   GH921104   GH923185   GH926889   GH928227   GH928682   GH930136   GH935262   M74428 AAA28655   U01842 AAA03718
UniProtKB/Swiss-Prot	P46863
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral midline   (Heck et al., 1993) cephalic furrow   (Heck et al., 1993) ventral nervous system | restricted   (Heck et al., 1993) embryonic stage 6   mesoderm anlage   (Heck et al., 1993) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & larval stage & pupal stage     (Stewart et al., 1991) adult stage | female   ovary   (Stewart et al., 1991) adult stage | male   testis   (Stewart et al., 1991)
Additional Descriptive Data
	Klp61F transcripts were detected throughout the cytoplasm of unfertilized eggs. The maternal RNA persists until cellularization. During gastrulation, Klp61F transcripts are first detected in a wide band of ventral cells corresponding to the invaginating presumptive mesoderm. They are also seen in a more dorsal strip of cells and underneath the pole cells. Later embryos show expression in the head, along the cephalic furrow, along the ventral midline, and within the posterior region. After germ band retraction, expression is limited to the cells of the head and ventral neurogenic region. Regions of expression are well correlated with domains of cell division. (Heck et al., 1993) Klp61F transcripts are expressed throughout development. In adults, they are detected only in the gonads. (Stewart et al., 1991)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
	Klp61F protein localizes to the structural domains of the mitotic spindle throughout mitosis. (Barton et al., 1995) Throughout mitosis, nonphosphorylated Klp61F protein is diffuse and cytoplasmic. Phosphorylated Klp61F shows a very different distribution. During interphase and prophase, the staining is punctate and concentrated in the nucleus. Occasional faint staining is seen in the centrosome in prophase. By metaphase, the staining localizes to the spindle and appears filamentous. Phospho-Klp61F is particularly concentrated on the half spindles and densely stained bridges between the spindles in the midzone. Lower level staining occurs at the oles and grainy cytoplasmic staining is observed. During anaphase A, the staining resembles metaphase. During anaphase B, nearly all the phospho-Klp61F is present in the spindle between the separating nuclei. Filaments of phospho-Klp61F protein staining cross the midzone while little or no staining is associated with the spindle poles and astral microtubules. During telophase, Klp61F staining is most pronounced on two adjacent patches on the remnants of the central spindle. EM studies suggest that the phospho-Klp61F motors form crosslinks between microtubules within interpolar microt bule bundles. (Sharp et al., 1999)
Marker for
Subcellular Localization
CV Term	spindle centrosome (Sharp et al., 1999) spindle (Barton et al., 1995)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0004378    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0004378 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0004378 expression level Developmental Stage   Expression Level embryo 00-02hr    4484 embryo 02-04hr    6005 embryo 04-06hr    6312 embryo 06-08hr    5818 embryo 08-10hr    4451 embryo 10-12hr    1735 embryo 12-14hr    1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr    2180 embryo 22-24hr    1788 larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr    1991 white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day    1990 adult female 30day    2076 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4484) embryo 02-04hr  (6005) embryo 04-06hr  (6312) embryo 06-08hr  (5818) embryo 08-10hr  (4451) embryo 10-12hr  (1735) embryo 12-14hr  (1424) embryo 14-16hr  (1216) embryo 16-18hr  (1001) embryo 18-20hr  (500) embryo 20-22hr  (2180) embryo 22-24hr  (1788) larva L1    82 larva L2  (129) larva L3 12hr old  (280) larva L3 puffstage 1-2  (615) larva L3 puffstage 3-6  (845) larva L3 puffstage 7-9  (942) white prepupae new  (853) white prepupae 12hr  (1991) white prepupae 24hr  (1340) pupae 2d postWPP  (276) pupae 3d postWPP  (150) pupae 4d postWPP  (177) adult male 01day  (178) adult male 05day  (310) adult male 30day  (238) adult female 01day  (771) adult female 05day  (1990) adult female 30day  (2076) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4484) embryo 02-04hr  (6005) embryo 04-06hr  (6312) embryo 06-08hr  (5818) embryo 08-10hr  (4451) embryo 10-12hr  (1735) embryo 12-14hr  1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr  (2180) embryo 22-24hr  (1788) larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr  (1991) white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day  (1990) adult female 30day  (2076) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4484 embryo 02-04hr    6005 embryo 04-06hr    6312 embryo 06-08hr    5818 embryo 08-10hr    4451 embryo 10-12hr    1735 embryo 12-14hr    1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr    2180 embryo 22-24hr    1788 larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr    1991 white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day    1990 adult female 30day    2076 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4484 embryo 02-04hr    6005 embryo 04-06hr    6312 embryo 06-08hr    5818 embryo 08-10hr    4451 embryo 10-12hr    1735 embryo 12-14hr    1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr    2180 embryo 22-24hr    1788 larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr    1991 white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day    1990 adult female 30day    2076 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0004378 expression level Developmental Stage   Expression Level embryo 00-02hr    4484 embryo 02-04hr    6005 embryo 04-06hr    6312 embryo 06-08hr    5818 embryo 08-10hr    4451 embryo 10-12hr    1735 embryo 12-14hr    1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr    2180 embryo 22-24hr    1788 larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr    1991 white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day    1990 adult female 30day    2076 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4484) embryo 02-04hr  (6005) embryo 04-06hr  (6312) embryo 06-08hr  (5818) embryo 08-10hr  (4451) embryo 10-12hr  (1735) embryo 12-14hr  (1424) embryo 14-16hr  (1216) embryo 16-18hr  (1001) embryo 18-20hr  (500) embryo 20-22hr  (2180) embryo 22-24hr  (1788) larva L1    82 larva L2  129 larva L3 12hr old  (280) larva L3 puffstage 1-2  (615) larva L3 puffstage 3-6  (845) larva L3 puffstage 7-9  (942) white prepupae new  (853) white prepupae 12hr  (1991) white prepupae 24hr  (1340) pupae 2d postWPP  (276) pupae 3d postWPP  (150) pupae 4d postWPP  (177) adult male 01day  (178) adult male 05day  (310) adult male 30day  (238) adult female 01day  (771) adult female 05day  (1990) adult female 30day  (2076) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4484) embryo 02-04hr  (6005) embryo 04-06hr  (6312) embryo 06-08hr  (5818) embryo 08-10hr  (4451) embryo 10-12hr  1735 embryo 12-14hr  1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr  2180 embryo 22-24hr  1788 larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr  1991 white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day  1990 adult female 30day  2076 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4484 embryo 02-04hr    6005 embryo 04-06hr    6312 embryo 06-08hr    5818 embryo 08-10hr    4451 embryo 10-12hr    1735 embryo 12-14hr    1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr    2180 embryo 22-24hr    1788 larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr    1991 white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day    1990 adult female 30day    2076 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4484 embryo 02-04hr    6005 embryo 04-06hr    6312 embryo 06-08hr    5818 embryo 08-10hr    4451 embryo 10-12hr    1735 embryo 12-14hr    1424 embryo 14-16hr    1216 embryo 16-18hr    1001 embryo 18-20hr    500 embryo 20-22hr    2180 embryo 22-24hr    1788 larva L1    82 larva L2    129 larva L3 12hr old    280 larva L3 puffstage 1-2    615 larva L3 puffstage 3-6    845 larva L3 puffstage 7-9    942 white prepupae new    853 white prepupae 12hr    1991 white prepupae 24hr    1340 pupae 2d postWPP    276 pupae 3d postWPP    150 pupae 4d postWPP    177 adult male 01day    178 adult male 05day    310 adult male 30day    238 adult female 01day    771 adult female 05day    1990 adult female 30day    2076 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0004378    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0004378 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0004378 expression level Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0004378 expression level Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body no informative data Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass no informative data Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart no informative data Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland no informative data Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1.5   NA  Eye    1.7   NA  Brain    1.4   249.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1.8   NA  Crop    82.6   no informative data  Midgut    no informative data   no informative data  Hindgut    no informative data   7.5  Malpighian Tubules    4.6   no informative data  Fat Body    3.1   9.8  Salivary Gland    9.4   NA  Heart    no informative data   76.525  Trachea    NA   NA  VirginFemale Spermatheca    5   NA  InseminatedFemale Spermatheca    1.7   NA  Ovary    304.4   NA  Testis    79.2   NA  Male Accessory Gland    no informative data   no informative data  Carcass    6.9 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004378 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9191-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Klp61F Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 Klp61FGD14149 lethal (with Df(3L)3C7) Klp61F6D283 lethal (with Df(3L)bab-PG) Klp61F6D283 lethal | embryonic stage | recessive Klp61FMk2 lethal | larval stage (with Klp61F06836) Klp61F07012 lethal | larval stage (with Klp61F07012) Klp61F06836 lethal | larval stage | recessive Klp61F07012 lethal | pupal stage | recessive Klp61F07012 lethal | recessive Klp61F06345 Klp61F06836 Klp61F07012 Klp61FA9 Klp61FA67 Klp61FB14 Klp61FDL42 Klp61FH10 Klp61Furc-1 Klp61Furc-3 Klp61Furc-4 lethal | recessive | second instar larval stage Klp61FMk5 viable, with Klp61FUbi-p63E.T:Avic\GFP/Klp61F06836 Klp61F07012 viable (with Klp61F06836), with Klp61F07012 Klp61FUbi-p63E.T:Avic\GFP viable (with Klp61F07012) Klp61FUbi-p63E.T:Avic\GFP viable (with Klp61FUbi-p63E.T:Avic\GFP) Klp61F07012 viable (with Klp61FUbi-p63E.T:Avic\GFP), with Klp61F07012 Klp61F06836 Sterility Allele fertile, with Klp61FUbi-p63E.T:Avic\GFP/Klp61F06836 Klp61F07012 fertile (with Klp61F06836), with Klp61F07012 Klp61FUbi-p63E.T:Avic\GFP fertile (with Klp61F07012) Klp61FUbi-p63E.T:Avic\GFP fertile (with Klp61FUbi-p63E.T:Avic\GFP) Klp61F07012 fertile (with Klp61FUbi-p63E.T:Avic\GFP), with Klp61F07012 Klp61F06836 Other Phenotypes Allele cytokinesis defective Klp61F07012 mitotic cell cycle defective Klp61Furc-1 Klp61Furc-2 Klp61Furc-3 Klp61Furc-4 Klp61Fv27 mitotic cell cycle defective | embryonic stage 4 (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 mitotic cell cycle defective | embryonic stage 4 (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC mitotic cell cycle defective | larval stage Klp61F07012 mitotic cell cycle defective | pre-blastoderm stage Klp61F07012 mitotic cell cycle defective | pre-blastoderm stage (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 mitotic cell cycle defective | pre-blastoderm stage (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC mitotic cell cycle defective | recessive Klp61F06345 Klp61F06836 Klp61F07012 Klp61Furc-1 mitotic cell cycle defective | somatic clone | third instar larval stage Klp61FA9 Klp61FA67 Klp61FB14 Klp61FDL42 Klp61FH10 neuroanatomy defective | larval stage, with Scer\GAL41407 Klp61FGD14149 Phenotype manifest in Allele aster & neuroblast & larva Klp61F07012 aster & spermatocyte Klp61F07012 aster & spermatocyte (with Df(3L)bab-PG) Klp61F07012 aster & spermatocyte (with Klp61F06345) Klp61F07012 aster & spermatocyte (with Klp61F07012) Klp61F06345 astral microtubule Klp61FdsRNA.cGa centriole & spermatocyte Klp61F06345 Klp61F07012 centrosome & neuroblast & larva Klp61F07012 centrosome & spermatocyte Klp61F07012 centrosome & spermatocyte (with Df(3L)bab-PG) Klp61F07012 centrosome & spermatocyte (with Klp61F06345) Klp61F07012 centrosome & spermatocyte (with Klp61F07012) Klp61F06345 embryonic/larval brain Klp61F06345 Klp61F06836 Klp61F07012 Klp61Furc-1 Klp61Furc-3 Klp61Furc-4 embryonic/larval brain | somatic clone | third instar larval stage Klp61FA9 Klp61FA67 Klp61FB14 Klp61FH10 Klp61FDL42 embryonic/larval neuroblast Klp61F07012 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 Klp61FGD14149 embryonic/larval neuroblast | somatic clone | third instar larval stage Klp61FA9 Klp61FA67 Klp61FB14 Klp61FH10 Klp61FDL42 ganglion mother cell | larval stage, with Scer\GAL41407 Klp61FGD14149 imaginal disc | larval stage Klp61F06345 Klp61F06836 Klp61F07012 Klp61Furc-1 Klp61Furc-3 Klp61Furc-4 larval brain & nuclear chromosome & larva Klp61Fv27 meiotic telophase II & spermatocyte Klp61F07012 microtubule organizing center | pre-blastoderm stage (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 microtubule organizing center | pre-blastoderm stage (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC mitosis & nuclear chromosome Klp61FdsRNA.cGa mitotic cell cycle & spermatocyte Klp61F07012 nuclear chromosome Klp61F06345 Klp61F06836 Klp61F07012 nuclear lamina & neuroblast & larva Klp61F07012 Klp61Furc-1 nuclear lamina & spermatocyte Klp61F07012 S2 cell-line Klp61FdsRNA.cGa spermatid (with Klp61F07012) Klp61Furc-1 spermatid (with Klp61Furc-1) Klp61F07012 spermatocyte (with Klp61F07012) Klp61Furc-1 spermatocyte (with Klp61Furc-1) Klp61F07012 spermatocyte fusome Klp61F06345 Klp61F07012 spindle Klp61F07012 Klp61FdsRNA.cGa spindle & neuroblast & larva Klp61F07012 spindle & spermatocyte Klp61F07012 spindle & spermatocyte (with Df(3L)bab-PG) Klp61F07012 spindle & spermatocyte (with Klp61F06345) Klp61F07012 spindle & spermatocyte (with Klp61F07012) Klp61F06345 Klp61Furc-1 spindle & spermatocyte (with Klp61Furc-1) Klp61F07012 spindle | embryonic stage, with Klp61FUbi-p63E.T:Avic\GFP/Klp61F06836 Klp61F07012 spindle | embryonic stage (with Klp61F06836), with Klp61F07012 Klp61FUbi-p63E.T:Avic\GFP spindle | embryonic stage (with Klp61F07012) Klp61FUbi-p63E.T:Avic\GFP spindle | embryonic stage (with Klp61FUbi-p63E.T:Avic\GFP) Klp61F07012 spindle | embryonic stage (with Klp61FUbi-p63E.T:Avic\GFP), with Klp61F07012 Klp61F06836 spindle | pre-blastoderm stage Klp61F07012 spindle microtubule Klp61FdsRNA.cGa spindle microtubule | embryonic stage 4 Klp61F07012 spindle microtubule | embryonic stage 4 (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 spindle microtubule | embryonic stage 4 (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC spindle pole Klp61FdsRNA.cGa testis Klp61Furc-1
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of Klp61F Class Mutagen Stocks Known lesion Klp61F06345 hypomorphic allele - genetic evidence P-element activity 1 Yes Klp61F07012 hypomorphic allele - genetic evidence P-element activity 1 Yes Klp61Ff02870 piggyBac transposase 1 -- Klp61FMk2 ethyl methanesulfonate 1 -- Klp61FMk5 ethyl methanesulfonate 1 -- Klp61Furc-1 loss of function allele P-element activity 1 Yes Klp61F06836 hypomorphic allele - genetic evidence P-element activity 0 Yes Klp61F6D283 ethyl methanesulfonate 0 -- Klp61F7415 0 -- Klp61FA67 ethyl methanesulfonate 0 -- Klp61FA9 ethyl methanesulfonate 0 -- Klp61FB14 ethyl methanesulfonate 0 -- Klp61FDL42 ethyl methanesulfonate 0 -- Klp61FH10 ethyl methanesulfonate 0 -- Klp61Funspecified 0 -- Klp61Furc-2 ethyl methanesulfonate 0 -- Klp61Furc-3 ethyl methanesulfonate 0 -- Klp61Furc-4 ethyl methanesulfonate 0 -- Klp61Fv27 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 19 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Klp61F Class Mutagen Stocks Known lesion Klp61FGD14149 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Klp61FUbi-p63E.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes Klp61FGL00441 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Klp61FHMS00552 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Klp61FUbi-p63E.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Klp61F3YF.Ubi-p63E.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Klp61FdsRNA.cGa in vitro construct - RNAi 0 Yes Klp61FdsRNA.cLa in vitro construct - RNAi 0 Yes Klp61FKK100504 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Klp61FMtnA.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Klp61FS1050A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FS1050A.S1054A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FS949A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT1045A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT1045A.S1050A.S1054A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT933A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT933A.Ubi-p63E.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Klp61FT933D.Ubi-p63E.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Klp61FT:SV5\V5 in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)4231 (Wei et al., 2003) Df(3L)Ar14-8 (Spradling et al., 1999, Wilson et al., 1997) Df(3L)bab-PG (Spradling et al., 1999, Wilson et al., 2004) Df(3L)BSC311 (Christensen et al., 2007.10.29) Df(3L)BSC363 (Christensen et al., 2007.10.29) Df(3L)BSC431 (Christensen et al., 2008.4.15) Df(3L)BSC799 (Christensen et al., 2009.5.6)
Not disrupted in	Df(3L)Ar12-1 (Wilson et al., 1997) Df(3L)B67 (Wei et al., 2003)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14149} NA P{GD17447} NA P{KK100504} NA P{TRiP.GL00441} NA P{TRiP.HMS00552} NA characterization construct P{KLP61F.H} NA P{Ubi-Klp61F.GFP.T933A} NA P{Ubi-Klp61F.GFP.T933D} NA P{Ubi-Klp61F.GFP} NA P{Ubi-Myc-Klp61F.3YF} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Klp61Furc-1 No P{lArB}Klp61Fv27 No P{PZ}Klp61F06345 No P{PZ}Klp61F06836 No P{PZ}Klp61F07012 No miscellaneous insertions PBac{WH}Klp61Ff02870 NA
Gene Ontology: Function, Process & Cellular Component ( 28 unique terms )
Terms Based on Experimental Evidence ( 23 terms )
Molecular Function
CV term Evidence References
motor activity inferred from direct assay (Cole and Scholey, 1995)
Biological Process
CV term Evidence References
cell cycle inferred from mutant phenotype (Kiger et al., 2003) centrosome duplication inferred from mutant phenotype (Müller et al., 2010) centrosome separation inferred from mutant phenotype (Gandhi et al., 2004) Golgi organization inferred from mutant phenotype (Bard et al., 2006) microtubule bundle formation inferred from direct assay (Tao et al., 2006) mitosis inferred from mutant phenotype (Ducat et al., 2008) mitotic centrosome separation inferred from genetic interaction with ncd AND inferred from mutant phenotype (Sharp et al., 1999) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle stabilization inferred from mutant phenotype (Garcia et al., 2009) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) plus-end directed microtubule sliding inferred from direct assay (Tao et al., 2006) protein homotetramerization inferred from direct assay (Tao et al., 2006) protein secretion inferred from mutant phenotype (Bard et al., 2006)
Cellular Component
CV term Evidence References
colocalizes_with centrosome inferred from direct assay (Cheerambathur et al., 2008) cytoplasm inferred from direct assay (Cole and Scholey, 1995) endoplasmic reticulum inferred from direct assay (Bard et al., 2006) fusome inferred from direct assay (Wilson, 2005) Golgi apparatus inferred from direct assay (Bard et al., 2006) kinesin complex inferred from direct assay (Cole and Scholey, 1995) microtubule inferred from direct assay (Cheerambathur et al., 2008) microtubule associated complex inferred from direct assay (Cole and Scholey, 1995, Hughes et al., 2008) spindle inferred from direct assay (Cheerambathur et al., 2008)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821 (FlyBase Curators et al., 2004-) microtubule motor activity inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) plus-end-directed microtubule motor activity inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1995.9.1)
Biological Process
CV term Evidence References
microtubule-based movement inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) mitotic spindle organization non-traceable author statement (Swiss-Prot Project Members, 1995.9.1)
Cellular Component
CV term Evidence References
kinesin complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) plus-end kinesin complex inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1995.9.1) spindle non-traceable author statement (Swiss-Prot Project Members, 1995.9.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Klp61F allele Gene References Klp61F06345 ncd (Wilson et al., 2004) Klp61F07012 ncd (Wilson et al., 2004) Klp61Furc-1 ncd (Wilson et al., 2004) unspecified Klp67A (Gandhi et al., 2004) ncd (Sharp et al., 1999) Wk (Furman and Omel'ianchuk, 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63681 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004378 DroID - A comprehensive database of gene and protein interactions. • FBgn0004378 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38135
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10068 Dere\GG14613 Dgri\GH16010 Dmoj\GI12274 Dper\GL16080 Dpse\GA21600 Dsec\GM14227 Dsim\GD13489 Dvir\GJ13211 Dwil\GK11822 Dyak\GE20974
Curated drosophilid orthologs	Dmir\CG9191 (Thornton, 2005.12.20) Dpse\GA21600
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL014084-PA Aedes aegypti (mosquito) AGAP007502-PA Anopheles gambiae (mosquito) XP_623508.2 Apis mellifera (honey bee) XP_001946589.1 Acyrthosiphon pisum (pea aphid) At3g45850.1 Arabidopsis thaliana (wall cress) 125471 Branchiostoma floridae (lancelet) XP_001897034.1 Brugia malayi (roundworm nematode) BGIBMGA008726-PA Bombyx mori (silk worm) ENSBTAP00000012347 Bos taurus (bovine) CAL0001739 Candida albicans (a form of yeast) CN16279 Caenorhabditis brenneri (small nematode) CBP30325 Caenorhabditis briggsae (nematode) CE09600 Caenorhabditis elegans (worm) ENSCAFP00000011137 Canis familiaris (dog) Chro.60483 Cryptosporidium hominis (obligate parasite) JA04378 Caenorhabditis japonica (nematode) CMC157C Cyanidioschyzon merolae (red alga) cn07346 Cryptococcus neoformans (yeast-like fungus) cgd6_4210 Cryptosporidium parvum (protozoan parasite) CPIJ008622 Culex pipens (mosquito) ENSCPOP00000014564 Cavia porcellus (guinea pig) RP35338 Caenorhabditis remanei (nematode) 226254 Capitella spl (polychaete worm) DDB0201557 Dictyostelium discoideum (amoeba) DEHA2E05874g Debaryomyces Hansenii (yeast) 55076 Daphnia pulex (daphnia) ENSDARP00000006884 Danio rerio (zebra fish) ENSECAP00000005143 Equus caballus (horse) XP_649446 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000014726 Gymnopilus aculeatus (mushroom) ENSGALP00000010967 Gallus gallus (chicken) GL50803_16425 Giardia lamblia (flagellated protozoan) ENSP00000260731 Homo sapiens (human) 119832 Lottia gigantea (owl limpet) ENSMODP00000008027 Monodelphis domestica (gray opossum) ENSMMUP00000030625 Macaca mulatta (rhesus monkey) ENSMUSP00000012587 Mus musculus (house mouse) 238203 Nematostella vectensis (starlet anemone) XP_001600745.1 Nasonia vitripennis (wasp) LOC_Os05g02670.1 Oryza sativa (asian rice) PHUM000278-PA Pediculus humanus (human body louse) PP08007 Pristionchus pacificus (nematode) 158752 Physcomitrella patens (moss) ENSPPYP00000002878 Pongo pygmaeus (orangutan) 72583 Phytophthora ramorum (sudden oak death pathogen) 155999 Phytophthora sojae (root rot pathogen) 821577 Populus trichocarpa (black cottonwood) ENSPTRP00000004812 Pan troglodytes (chimpanzee) PVX_095355 Plasmodium vivax (protozoal parasite) ENSRNOP00000022555 Rattus norvegicus (norway rat) 5060008 Sorghum bicolor (sorghum) YBL063W Saccharomyces cerevisiae (budding yeast) SPAC25G10.07c Schizosaccharomyces pombe (fission yeast) NP_999660.1 Strongylocentrotus purpuratus (purple sea urchin) XP_967968.1 Tribolium castaneum (red flour beetle) ENSTNIP00000002275 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000030956 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000026683 Xenopus tropicalis (frog) YALI0F14179g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14475 NV15730 LH11266 LH23607 ISCW006455 FBgn0238258 FBgn0213833 FBgn0200437 FBgn0185200 FBgn0169153 JGI_V11_55076 JGI_V11_57725 JGI_V11_62900 FBgn0081586 FBgn0153684 FBgn0153683 FBgn0135031 FBgn0123481 FBgn0106869 FBgn0087109 CPIJ008622 BGIBMGA008726 ACYPI002268 GB14683 GB10927 AGAP007502 AAEL014084 PB21862 PHUM434560 GLEAN_04752 GLEAN_04751 GLEAN_14244
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	35509 w*; P{Ubi-Klp61F.GFP}3 35510 w*; P{Ubi-Klp61F.GFP}2 35508 Klp61Furc-1/TM6B, Tb1 32011 w*; P{KLP61F.H}3/TM3, Sb1 32012 P{PZ}Klp61F06345/TM6B, Tb1 11710 P{PZ}Klp61F07012 ry506/TM6B, Tb1 35804 y1 sc* v1; P{TRiP.GL00441}attP40 33685 y1 sc* v1; P{TRiP.HMS00552}attP2/TM3, Sb1 32013 mwh1 Klp61FMk5 rhove-1 red1 e*/TM6B, Tb1
Harvard	- PBac{WH}Klp61Ff02870
VDRC	v52548 w1118; P{GD14149}v52548 v52549 w1118; P{GD14149}v52549
	More stocks available...
Genomic Clones ( 1 )
	BACR17K17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 209 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD15641
Other clones	DPiM_LD15641 RT07324
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT18509 AT27717 GM09567 GM12362 GM13005 GM13405 LD04280 LD07059 LD10676 LD10984 LD11639 LD12480 LD12969 LD13384 LD14571 LD18121 LD18462 LD19008 LD21891 LD22078 LD22393 LD23022 LD25828 LD27186 LD30833 LD30871 LD31634 LD34033 LD34739 LD35311 LD36993 LD38277 LD40531 LD41672 LD43317 LD44922 LD45930 RE30012 RE31477 RE52968 RE72066 SD01713 SD03396 SD04243 SD08410 SD13543 SD20629
Other clones	12550 35283 60307 67492 73334 82427 106650 119580 174496 191732 214383.30 214567.25 524034 CK01.65B.G6 Dmel_dig_018049_0622_0864 EK053159 EK113520 EK146421 EK175134 EK195522 EK202534 EK239801 EK243127 EK243130 EK244244 EK258101 EK275612 EK279319 EK294222 EP09019 GM23817 RP003006101 RP003009330 RP003011933 RP003017837 RP003021049 RP003021440 RP003021505 RP003028562 RP003028670 RP003029989 RP003034081 RP003035229 RP003035712 RP003045429 RP003056741 RP003062463 RP003063792 RP003064831 RP003067191 RP003071085 RP003083297 RP003088977 RP003093940 RP003094310 RP003095529 RP003095934 RP003102736 RP003118198 RP003124115 RP003129626 RP003132374 RP003152866 RP003158455 RP003161489 RP003162692 RP003164816 RP003172510 RP003174042 RP003174805 RP003178629 RP003184507 RP003187977 RP003189508 RP003196386 RP003201870 RP003204732 RP003205618 RP003210239 RP003211806 RP003215851 RP003225446 RP003226634 RP003234528 RP003248537 RP003248998 RP003253432 RP003254280 RP003256955 RP003257203 RP003258394 RP003260593 RP003261028 RP003262747 RP003268578 RP003271117 RP003273429 RP003274368 RP003274666 RP003282773 RP003294320 RP003296268 RP003309839 RP003313276 RP003337038 RP003341948 RP003342838 RP003344716 RP003348678 RP003351190 RP003360832 RP003363927 RP003367220 RP003370641 RP003378568 RP003385453 RP003396235 RP003396553 RP003401179 RP003412543 RP003416400 RP003425593 RP003430143 RP003432505 RP003446568 RP003446608 RP003449162 RP003451730 RP003462901 RP003469121 RP003469235 RP003485772 RP003490759 RP003492557 RP003494629 RP003506343 RP003514825 RP003517800 RP003527797 RP003533041 RP003535442 RP003542686 RP003546372 RP003556550 RP003556915 RP003556998 RP003568238 RP003569091 RP003570357 RP003581517 RP003581926 RP003613559 RP003613954 RP003615697 RP003625314 RP003628611 RP003637468 RP003656758 RP003675623
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004378 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38135
Antibody Information
	polyclonal (Sharp et al., 1999, Barton et al., 1995, Brust-Mascher et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Klp61F Krp130 (Scholey, 1999.5.5)
Additional comments	"CG32318" is a putative chimeric gene derived from the "Klp61F" and "Psf1" genes (where coding sequences of the two parental genes contribute to the coding sequence of the chimeric gene). (Rogers et al., 2009)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) dsRNA directed against Klp61F inhibits protein secretion in S2 cells and causes aggregation and swelling of the Golgi membranes in more than 50% of the treated cells. (Bard et al., 2006) dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion. (Bard et al., 2006) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: during mitosis, centrosome separation is prevented, resulting in monopolar spindles. (Gandhi et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Spindle pole movements in embryos are directed by a temporally coordinated balance of forces generated by three mitotic motors; cytoplasmic dynein, Klp61F and ncd. Dynein acts to move the poles apart throughout mitosis, and this activity is augmented by Klp61F after the fenestration of the nuclear envelope, which occurs at the onset of prometaphase. ncd generates forces that pull the poles together between interphase and metaphase, antagonising the activity of both cytoplasmic dynein and Klp61F and serving as a brake for spindle assembly. (Sharp et al., 2000) Klp61F cycles between spindles during mitosis and fusomes during interphase. (Wilson, 1999) Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland. (Harvie et al., 1998) Klp61F is involved in the control of cell division. (Omel'yanchuk et al., 1997) Klp61F protein, a homotetrameric BimC-related kinesin, consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends. Such a bipolar 'minifilament' could crosslink spindle microtubules and slide them relative to one another. (Kashina et al., 1996) Antibody staining reveals multiple bimC family members are expressed in Drosophila. Klp61F is an essential gene and biochemical and functional analysis of the protein demonstrates it is not functionally redundant for centrosome separation during neuroblast separation. (Barton et al., 1995) Relationship to cloned Klp genes not known. Purified Klp61F behaves as a homotetrameric complex of four subunits that moves toward the plus ends of microtubule tracts at approximately 0.04μm/s. (Cole and Scholey, 1995) Encodes a 130kD kinesin related protein that behaves as a homotetramer and moves towards the plus ends of microtubules. (Cole and Scholey, 1995) Identified as a 130kD polypeptide in an ATP MAPs 1-24 hour embryonic fraction. A 490kD homotetrameric complex consisting of 4 130kD subunits display a 'slow' plus-end directed motor activity capable of moving single microtubules. (Cole et al., 1994) The sequence of the Klp61F protein has been compared with the sequences of a variety of kinesin family proteins. (Goodson et al., 1994) Klp61F has been cloned, partially sequenced and its expression pattern analysed. (Stewart et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057470 RefSeq (Proteins) • NP_476818 DDBJ / EMBL / GenBank • AA440767 AAA03718 AAA28655 AAF47458 AAL39587 ABG91086 ABG91087 AC004489 AC005847 AQ026225 AW942371 AY069442 AY729990 CZ485426 DQ782381 DQ782382 M74428 U01842 Entrez Gene - A searchable database of RefSeq genes. • 38135 UniProtKB/Swiss-Prot • P46863
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Kinesin, motor domain (IPR001752) Kinesin, motor region, conserved site (IPR019821)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63681 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004378 DroID - A comprehensive database of gene and protein interactions. • FBgn0004378 DRSC - Results from RNAi screens. • FBgn0004378 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004378 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9191-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004378 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38135 InParanoid A subset of ortholog calls from InParanoid. • AAEL014084-PA Aedes aegypti (mosquito) AGAP007502-PA Anopheles gambiae (mosquito) XP_623508.2 Apis mellifera (honey bee) XP_001946589.1 Acyrthosiphon pisum (pea aphid) At3g45850.1 Arabidopsis thaliana (wall cress) 125471 Branchiostoma floridae (lancelet) XP_001897034.1 Brugia malayi (roundworm nematode) BGIBMGA008726-PA Bombyx mori (silk worm) ENSBTAP00000012347 Bos taurus (bovine) CAL0001739 Candida albicans (a form of yeast) CN16279 Caenorhabditis brenneri (small nematode) CBP30325 Caenorhabditis briggsae (nematode) CE09600 Caenorhabditis elegans (worm) ENSCAFP00000011137 Canis familiaris (dog) Chro.60483 Cryptosporidium hominis (obligate parasite) JA04378 Caenorhabditis japonica (nematode) CMC157C Cyanidioschyzon merolae (red alga) cn07346 Cryptococcus neoformans (yeast-like fungus) cgd6_4210 Cryptosporidium parvum (protozoan parasite) CPIJ008622 Culex pipens (mosquito) ENSCPOP00000014564 Cavia porcellus (guinea pig) RP35338 Caenorhabditis remanei (nematode) 226254 Capitella spl (polychaete worm) DDB0201557 Dictyostelium discoideum (amoeba) DEHA2E05874g Debaryomyces Hansenii (yeast) 55076 Daphnia pulex (daphnia) ENSDARP00000006884 Danio rerio (zebra fish) ENSECAP00000005143 Equus caballus (horse) XP_649446 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000014726 Gymnopilus aculeatus (mushroom) ENSGALP00000010967 Gallus gallus (chicken) GL50803_16425 Giardia lamblia (flagellated protozoan) ENSP00000260731 Homo sapiens (human) 119832 Lottia gigantea (owl limpet) ENSMODP00000008027 Monodelphis domestica (gray opossum) ENSMMUP00000030625 Macaca mulatta (rhesus monkey) ENSMUSP00000012587 Mus musculus (house mouse) 238203 Nematostella vectensis (starlet anemone) XP_001600745.1 Nasonia vitripennis (wasp) LOC_Os05g02670.1 Oryza sativa (asian rice) PHUM000278-PA Pediculus humanus (human body louse) PP08007 Pristionchus pacificus (nematode) 158752 Physcomitrella patens (moss) ENSPPYP00000002878 Pongo pygmaeus (orangutan) 72583 Phytophthora ramorum (sudden oak death pathogen) 155999 Phytophthora sojae (root rot pathogen) 821577 Populus trichocarpa (black cottonwood) ENSPTRP00000004812 Pan troglodytes (chimpanzee) PVX_095355 Plasmodium vivax (protozoal parasite) ENSRNOP00000022555 Rattus norvegicus (norway rat) 5060008 Sorghum bicolor (sorghum) YBL063W Saccharomyces cerevisiae (budding yeast) SPAC25G10.07c Schizosaccharomyces pombe (fission yeast) NP_999660.1 Strongylocentrotus purpuratus (purple sea urchin) XP_967968.1 Tribolium castaneum (red flour beetle) ENSTNIP00000002275 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000030956 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000026683 Xenopus tropicalis (frog) YALI0F14179g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/klp61f.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38135 modMine - Data generated by the modENCODE project. • FBgn0004378 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14475 NV15730 LH11266 LH23607 ISCW006455 FBgn0238258 FBgn0213833 FBgn0200437 FBgn0185200 FBgn0169153 JGI_V11_55076 JGI_V11_57725 JGI_V11_62900 FBgn0081586 FBgn0153684 FBgn0153683 FBgn0135031 FBgn0123481 FBgn0106869 FBgn0087109 CPIJ008622 BGIBMGA008726 ACYPI002268 GB14683 GB10927 AGAP007502 AAEL014084 PB21862 PHUM434560 GLEAN_04752 GLEAN_04751 GLEAN_14244
Synonyms & Secondary IDs ( 32 )
Reported As
Symbol Synonym	6345 (Harvie et al., 1998) CG9191 (Goldstein and Gunawardena, 2000, Goshima et al., 2007, Ducat et al., 2008, Hughes et al., 2008, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) CG9191-PA (Rogers et al., 2009) DmKLP61F (Gadde and Heald, 2004, Matthies et al., 2001, Hirokawa et al., 1998) DmKlp61F (Kim and Endow, 2000, Moore and Endow, 1996) DmKRP130 (Moore and Endow, 1996) KIP8 (Miki et al., 2001) KLP2 (Wilson et al., 1992, Stewart et al., 1991) Klp2   KLP61 (Gonzalez et al., 1998) KLP61f (Kai et al., 2005) klp61f (Wilson et al., 2004, Wilson et al., 2004.8.21, Cheerambathur et al., 2008) KLP61F (Kwon and Scholey, 2004, Adams et al., 2001, Wilson, 1999, Wilson, 1999, Glover et al., 1998, Compton, 1998, Kashina et al., 1997, Wilson and Borisy, 1996, Kashina et al., 1996, Barton et al., 1994, Heck et al., 1993, Wilson, 2005, Silverman-Gavrila and Wilde, 2006, Tao et al., 2006, Lawrence et al., 2002, Cheerambathur et al., 2008, Simmons and Saighal, 2005, van den Wildenberg et al., 2008, Brust-Mascher et al., 2009, Vendra et al., 2007, Civelekoglu-Scholey et al., 2010, Sommi et al., 2010, Tao and Scholey, 2010) Klp61F (De Lucia et al., 2005, Christensen et al., 2007.10.29, Christensen et al., 2007.10.29, Goshima and Vale, 2005, Goshima et al., 2007, Christensen et al., 2008.4.15, Kim et al., 2007, Georlette et al., 2007, Somma et al., 2008, Rogers et al., 2009, Ducat et al., 2008, Christensen et al., 2009.5.6, Slack et al., 2006, Vale et al., 2009, Wei et al., 2003, Anholt and Williams, 2010, Goldstein, 2010.9.10, Lakämper et al., 2010, Liu et al., 2011, Neumüller et al., 2011) klp61F (Gaspar and Szabad, 2009, Garcia et al., 2009) Klp61f (Casper and Van Doren, 2009) Klp61F/KRP130 (Endow, 1999) KRP130 (Kashina et al., 1997) Krp130   KRP130 (Cole and Scholey, 1995, Kashina et al., 1996, Kashina et al., 1995, Cole et al., 1994, Cole et al., 1994) KRP(130) (Cole and Scholey, 1995) l(3)6D283 (Wei et al., 2003) l(3)07012   l(3)Klp61F (Wei et al., 2003) urc   urcn (Wilson and Borisy, 1995, Wilson and Fuller, 1993)
Name Synonym	kinesin-5 (Cheerambathur et al., 2008, Garcia et al., 2009, Civelekoglu-Scholey et al., 2010, Tao and Scholey, 2010) kinesin-5 motor (Vale et al., 2009) kinesin-like protein (Goldstein, 1993.9.15) Kinesin-like protein at 61F   Kinesin-related protein-130kD (Cole and Scholey, 1995) urchin (Wilson, 1994, Wilson and Fuller, 1991, Wilson and Borisy, 1995, Goodson et al., 1994, Wilson et al., 1992, )
Secondary FlyBase IDs
	FBgn0004923 FBgn0010911 FBgn0015744
References ( 141 )
Generate a list of	All references relating to this gene ( 141 )
List References by type	Research paper  ( 61 ) Supplementary material  ( 9 ) Review  ( 24 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 17 ) Abstract  ( 16 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Letter  ( 1 ) Computer file  ( 4 ) Protein sequence record  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 7 )
	Liu et al., 2011, J. Biol. Chem. 286(8): 6201--6210 Loop 5-directed Compounds Inhibit Chimeric Kinesin-5 Motors: IMPLICATIONS FOR CONSERVED ALLOSTERIC MECHANISMS. [FBrf0213083] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Anholt and Williams, 2010, Chem. Senses 35(1): 21--30 The soluble proteome of the Drosophila antenna. [FBrf0209552] Civelekoglu-Scholey et al., 2010, J. Cell Biol. 188(1): 49--68 Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope. [FBrf0209681] Lakämper et al., 2010, J. Mol. Biol. 399(1): 1--8 The effect of monastrol on the processive motility of a dimeric kinesin-5 head/kinesin-1 stalk chimera. [FBrf0210839] Müller et al., 2010, EMBO J. 29(19): 3344--3357 Proteomic and functional analysis of the mitotic Drosophila centrosome. [FBrf0212030] Sommi et al., 2010, J. Cell Sci. 123(11): 1862--1872 A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophila embryos. [FBrf0210830] Show all research papers ...
Recent reviews ( 1 )
	Tao and Scholey, 2010, Methods 51(2): 233--241 Purification and assay of mitotic motors. [FBrf0211030] Show all reviews ...