• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Klp61F

General Information
Symbol	Dmel\Klp61F	Species	D. melanogaster
Name	Kinesin-like protein at 61F	Annotation symbol	CG9191
Feature type	protein_coding_gene	FlyBase ID	FBgn0004378
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	DmKLP61F, KRP130, urcn, KLP2
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	61F4-61F4	Sequence location	3L:1,241,568..1,245,910 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Kinesin-like protein at 61F is referred to in FlyBase by the symbol Dmel\Klp61F (CG9191, FBgn0004378). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: motor activity. There is experimental evidence for 13 unique biological process terms, many of which group under: cellular component organization or biogenesis; cell cycle; cell cycle process; organelle organization; cellular component movement; localization; biological regulation; system development; macromolecular complex subunit organization; microtubule bundle formation; microtubule-based movement; establishment of protein localization. 39 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; anatomical structure; organelle; spindle; intracellular organelle part; spindle microtubule; nuclear part; cytoplasmic part; organ system subdivision. It has one annotated transcript and one annotated polypeptide. Protein features are: Kinesin, motor domain; Kinesin, motor region, conserved site; Kinesin-associated microtubule-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1624620_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Klp61F. Gene sequence location is 3L:1241568..1245910.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 61F4-61F4   Limits computationally determined from genome sequence between P{PZ}l(3)0596705967 and P{PZ}l(3)0264002640
Experimentally Determined Cytological Location
Cytogenetic map Notes References 61F3-61F4   (determined by in situ hybridisation)   (Spradling et al., 1999) 61F-61F   (determined by in situ hybridisation)   (Omel'yanchuk et al., 1997) 61F1-61F8   61F5--6   (BDGP Project Members, 1994-1999) 61F3-61F4   (determined by in situ hybridisation)   (Heck et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Klp61F for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Klp61F-RA FBtr0072733  NM_057470   3871   1066
Additional Transcript Data & Comments
Reported size (kB)	3.9, 3.7 (northern blot) (Stewart et al., 1991) 3.7 (longest cDNA) (Heck et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Klp61F-PA   FBpp0072616   121.2   1066   6.39     NP_476818   AAF47458
Additional Polypeptide Data & Comments
Reported size (kDa)	1066 (aa) (Heck et al., 1993) 130 (kD observed) (Cole et al., 1994) 120 (kD observed) (Barton et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Kinesin-associated microtubule-binding domain (IPR025901) Kinesin, motor domain (IPR001752) Kinesin, motor region, conserved site (IPR019821) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201190   AA263815   AA391627   AA391713   AA391978   AA392036   AA438651   AA438977   AA439890   AA440767   AA538841   AA539314   AA539786   AA735699   AA801784   AA803527   AA803957   AA817061   AA817215   AA940618   AA940782   AA941605   AA941872   AA950728   AA950760   AA951257   AA979456   AA990827   AE014296 AAF47458   AC004489   AC005847   AI455672   AI456846   AI511793   AI512850   AI513910   AI515955   AI518651   AI520386   AI530932   AI532042   AI532659   AI542240   AQ026225   AW942371   AY069442 AAL39587   AY729990   BF491061   BF502702   BG635989   BI234322   BI235593   BI354371   BI367250   BI578223   BI638360   BT124785   CO177325   CO266434   CO274192   CO293307   CO299347   CO303911   CO303914   CO304278   CO308414   CO315257   CO318956   CO319606   CO325072   CO328333   CO329944   CZ485426   DQ782381 ABG91086   DQ782382 ABG91087   EC076596   EC083499   EC084477   EC085824   EC086374   EC201850   EC203029   EC207150   EC215784   EC215785   EC224228   EC250751   EC251891   EC258823   EC265606   EV580197   GH747759   GH747803   GH747815   GH747839   GH748435   GH748455   GH748661   GH753437   GH753811   GH754713   GH754928   GH755246   GH757440   GH761358   GH771009   GH771179   GH771592   GH772031   GH772900   GH773873   GH774095   GH776507   GH777041   GH777433   GH778696   GH781212   GH783257   GH783262   GH784838   GH785140   GH787060   GH788023   GH789781   GH789920   GH790967   GH792145   GH793188   GH794797   GH795890   GH802442   GH805004   GH805590   GH805615   GH806021   GH809352   GH810380   GH810923   GH811079   GH814948   GH816251   GH822265   GH823502   GH825642   GH826321   GH829840   GH829887   GH830176   GH830317   GH830514   GH831288   GH831553   GH835778   GH838160   GH838418   GH839984   GH840307   GH840901   GH841064   GH842530   GH842654   GH843105   GH844871   GH845111   GH845575   GH846368   GH847352   GH852865   GH857291   GH857565   GH859461   GH862613   GH863441   GH865006   GH865987   GH867576   GH869853   GH870033   GH870661   GH870818   GH871875   GH875640   GH878013   GH878803   GH880389   GH881020   GH882612   GH883954   GH884893   GH886404   GH889767   GH890961   GH892995   GH893841   GH899862   GH903288   GH903653   GH906191   GH907535   GH909714   GH910633   GH911682   GH912019   GH914978   GH915194   GH916457   GH916589   GH917181   GH917188   GH917251   GH917461   GH917912   GH921104   GH923185   GH926889   GH928227   GH928682   GH930136   GH935262   M74428 AAA28655   U01842 AAA03718
UniProtKB/Swiss-Prot	P46863
UniProtKB/TrEMBL	Q0PQ31 Q0PQ30
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral midline   (Heck et al., 1993) cephalic furrow   (Heck et al., 1993) ventral nervous system | restricted   (Heck et al., 1993) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 6   mesoderm anlage   (Heck et al., 1993) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic central brain surface glia   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic/larval lymph gland   (Fisher et al., 2012) gonad   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & larval stage & pupal stage     (Stewart et al., 1991) adult stage | female   ovary   (Stewart et al., 1991) adult stage | male   testis   (Stewart et al., 1991)
Additional Descriptive Data
	Klp61F transcripts were detected throughout the cytoplasm of unfertilized eggs. The maternal RNA persists until cellularization. During gastrulation, Klp61F transcripts are first detected in a wide band of ventral cells corresponding to the invaginating presumptive mesoderm. They are also seen in a more dorsal strip of cells and underneath the pole cells. Later embryos show expression in the head, along the cephalic furrow, along the ventral midline, and within the posterior region. After germ band retraction, expression is limited to the cells of the head and ventral neurogenic region. Regions of expression are well correlated with domains of cell division. (Heck et al., 1993) Klp61F transcripts are expressed throughout development. In adults, they are detected only in the gonads. (Stewart et al., 1991)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
	Klp61F protein localizes to the structural domains of the mitotic spindle throughout mitosis. (Barton et al., 1995) Throughout mitosis, nonphosphorylated Klp61F protein is diffuse and cytoplasmic. Phosphorylated Klp61F shows a very different distribution. During interphase and prophase, the staining is punctate and concentrated in the nucleus. Occasional faint staining is seen in the centrosome in prophase. By metaphase, the staining localizes to the spindle and appears filamentous. Phospho-Klp61F is particularly concentrated on the half spindles and densely stained bridges between the spindles in the midzone. Lower level staining occurs at the oles and grainy cytoplasmic staining is observed. During anaphase A, the staining resembles metaphase. During anaphase B, nearly all the phospho-Klp61F is present in the spindle between the separating nuclei. Filaments of phospho-Klp61F protein staining cross the midzone while little or no staining is associated with the spindle poles and astral microtubules. During telophase, Klp61F staining is most pronounced on two adjacent patches on the remnants of the central spindle. EM studies suggest that the phospho-Klp61F motors form crosslinks between microtubules within interpolar microt bule bundles. (Sharp et al., 1999)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with centrosome inferred from direct assay (Cheerambathur et al., 2008) cytoplasm inferred from direct assay (Cole and Scholey, 1995) endoplasmic reticulum inferred from direct assay (Bard et al., 2006) fusome inferred from direct assay (Wilson, 2005) Golgi apparatus inferred from direct assay (Bard et al., 2006) kinesin complex inferred from direct assay (Cole and Scholey, 1995) microtubule inferred from direct assay (Cheerambathur et al., 2008) microtubule associated complex inferred from direct assay (Cole and Scholey, 1995, Hughes et al., 2008) spindle inferred from direct assay (Cheerambathur et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    249.8 Larval Midgut    15.2 Larval Hindgut    34.9 Larval Malpighian Tubules    7.5 Larval Fat Body    36.1 Larval Salivary Gland    9.8 Larval Trachea    76.525 Larval Carcass    74.725 Adult Head    1.5 Adult Eye    1.7 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    1.8 Adult Crop    82.6 Adult Midgut    12.6 Adult Hindgut    36.1 Adult Malpighian Tubules    4.6 Adult Fat Body    3.1 Adult Salivary Gland    9.4 Adult Heart    19.8 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    1.7 Adult Ovary    304.4 Adult Testis    79.2 Adult Male Accessory Gland    11.3 Adult Carcass    6.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1.5   NA  Eye    1.7   NA  Brain    1.4   249.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1.8   NA  Crop    82.6   no informative data  Midgut    no informative data   no informative data  Hindgut    no informative data   7.5  Malpighian Tubules    4.6   no informative data  Fat Body    3.1   9.8  Salivary Gland    9.4   NA  Heart    no informative data   76.525  Trachea    NA   NA  VirginFemale Spermatheca    5   NA  InseminatedFemale Spermatheca    1.7   NA  Ovary    304.4   NA  Testis    79.2   NA  Male Accessory Gland    no informative data   no informative data  Carcass    6.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    66 ovary, mated 4-day female    55 testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (32) central nervous system, larvae L3  26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female  (66) ovary, mated 4-day female  (55) testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    66 ovary, mated 4-day female    55 testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    66 ovary, mated 4-day female    55 testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    66 ovary, mated 4-day female    55 testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  32 central nervous system, larvae L3  26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female  (66) ovary, mated 4-day female  (55) testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    66 ovary, mated 4-day female    55 testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    26 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    12 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    66 ovary, mated 4-day female    55 testis, mated 4-day male    9 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    80 embryo 04-06hr    84 embryo 06-08hr    77 embryo 08-10hr    59 embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day    26 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (59) embryo 02-04hr  (80) embryo 04-06hr  (84) embryo 06-08hr  (77) embryo 08-10hr  (59) embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr  (29) embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr  26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day  26 adult female 30day  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    80 embryo 04-06hr    84 embryo 06-08hr    77 embryo 08-10hr    59 embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day    26 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    80 embryo 04-06hr    84 embryo 06-08hr    77 embryo 08-10hr    59 embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day    26 adult female 30day    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    80 embryo 04-06hr    84 embryo 06-08hr    77 embryo 08-10hr    59 embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day    26 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (59) embryo 02-04hr  (80) embryo 04-06hr  (84) embryo 06-08hr  (77) embryo 08-10hr  (59) embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr  29 embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr  26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day  26 adult female 30day  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    80 embryo 04-06hr    84 embryo 06-08hr    77 embryo 08-10hr    59 embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day    26 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    80 embryo 04-06hr    84 embryo 06-08hr    77 embryo 08-10hr    59 embryo 10-12hr    23 embryo 12-14hr    19 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    1 larva L2    2 larva L3 12hr old    4 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    26 white prepupae 24hr    18 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    3 adult female 01day    10 adult female 05day    26 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    42 embryonic 1182-4H    79 embryonic GM2    41 embryonic Kc167    23 embryonic S1    48 embryonic S3    58 leg disc CME L1    60 wing disc CME-W2    46 wing disc ML-DmD8    53 wing disc ML-DmD9    81 wing disc ML-DmD16-c3    91 wing disc ML-DmD21    36 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    52 antennal disc ML-DmD20-c5    58 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    24 tumorous blood cells mbn2    44 ovary fGS/OSS    90 ovary OSC    81 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (108) Schneider line 2 Sg4  (42) embryonic 1182-4H  (79) embryonic GM2  (41) embryonic Kc167    23 embryonic S1  (48) embryonic S3  (58) leg disc CME L1  (60) wing disc CME-W2  (46) wing disc ML-DmD8  (53) wing disc ML-DmD9  (81) wing disc ML-DmD16-c3  (91) wing disc ML-DmD21  (36) wing disc ML-DmD32  (53) haltere disc ML-DmD17-c3  (127) eye-antennal disc ML-DmD11  (52) antennal disc ML-DmD20-c5  (58) mixed discs ML-DmD4-c1  (60) CNS ML-DmBG1-c1  (50) CNS ML-DmBG2-c2    24 tumorous blood cells mbn2  (44) ovary fGS/OSS  (90) ovary OSC  (81) ovary OSS  (59) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (108) Schneider line 2 Sg4    42 embryonic 1182-4H    79 embryonic GM2    41 embryonic Kc167    23 embryonic S1    48 embryonic S3    58 leg disc CME L1    60 wing disc CME-W2    46 wing disc ML-DmD8    53 wing disc ML-DmD9    81 wing disc ML-DmD16-c3    91 wing disc ML-DmD21    36 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3  (127) eye-antennal disc ML-DmD11    52 antennal disc ML-DmD20-c5    58 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    24 tumorous blood cells mbn2    44 ovary fGS/OSS    90 ovary OSC    81 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    42 embryonic 1182-4H    79 embryonic GM2    41 embryonic Kc167    23 embryonic S1    48 embryonic S3    58 leg disc CME L1    60 wing disc CME-W2    46 wing disc ML-DmD8    53 wing disc ML-DmD9    81 wing disc ML-DmD16-c3    91 wing disc ML-DmD21    36 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    52 antennal disc ML-DmD20-c5    58 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    24 tumorous blood cells mbn2    44 ovary fGS/OSS    90 ovary OSC    81 ovary OSS    59 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    42 embryonic 1182-4H    79 embryonic GM2    41 embryonic Kc167    23 embryonic S1    48 embryonic S3    58 leg disc CME L1    60 wing disc CME-W2    46 wing disc ML-DmD8    53 wing disc ML-DmD9    81 wing disc ML-DmD16-c3    91 wing disc ML-DmD21    36 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    52 antennal disc ML-DmD20-c5    58 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    24 tumorous blood cells mbn2    44 ovary fGS/OSS    90 ovary OSC    81 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (108) Schneider line 2 Sg4  (42) embryonic 1182-4H  (79) embryonic GM2  (41) embryonic Kc167    23 embryonic S1  (48) embryonic S3  (58) leg disc CME L1  (60) wing disc CME-W2  (46) wing disc ML-DmD8  (53) wing disc ML-DmD9  (81) wing disc ML-DmD16-c3  (91) wing disc ML-DmD21  (36) wing disc ML-DmD32  (53) haltere disc ML-DmD17-c3  (127) eye-antennal disc ML-DmD11  (52) antennal disc ML-DmD20-c5  (58) mixed discs ML-DmD4-c1  (60) CNS ML-DmBG1-c1  (50) CNS ML-DmBG2-c2    24 tumorous blood cells mbn2  (44) ovary fGS/OSS  (90) ovary OSC  (81) ovary OSS  (59) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  108 Schneider line 2 Sg4    42 embryonic 1182-4H    79 embryonic GM2    41 embryonic Kc167    23 embryonic S1    48 embryonic S3    58 leg disc CME L1    60 wing disc CME-W2    46 wing disc ML-DmD8    53 wing disc ML-DmD9    81 wing disc ML-DmD16-c3    91 wing disc ML-DmD21    36 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3  127 eye-antennal disc ML-DmD11    52 antennal disc ML-DmD20-c5    58 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    24 tumorous blood cells mbn2    44 ovary fGS/OSS    90 ovary OSC    81 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    42 embryonic 1182-4H    79 embryonic GM2    41 embryonic Kc167    23 embryonic S1    48 embryonic S3    58 leg disc CME L1    60 wing disc CME-W2    46 wing disc ML-DmD8    53 wing disc ML-DmD9    81 wing disc ML-DmD16-c3    91 wing disc ML-DmD21    36 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    52 antennal disc ML-DmD20-c5    58 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    24 tumorous blood cells mbn2    44 ovary fGS/OSS    90 ovary OSC    81 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    28 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult  (34) Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3  (30) Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (28) Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    28 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    28 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    28 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult  (34) Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3  30 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  28 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    28 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    7 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    21 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    28 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD15641 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004378 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9191-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Klp61F Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Klp61FGD14149 lethal (with Df(3L)3C7) Klp61F6D283 lethal (with Df(3L)bab-PG) Klp61F6D283 lethal | embryonic stage | recessive Klp61FMk2 lethal | larval stage (with Klp61F06836) Klp61F07012 lethal | larval stage (with Klp61F07012) Klp61F06836 lethal | larval stage | recessive Klp61F07012 lethal | pupal stage | recessive Klp61F07012 lethal | recessive Klp61F06345 Klp61F06836 Klp61F07012 Klp61FA9 Klp61FA67 Klp61FB14 Klp61FDL42 Klp61FH10 Klp61Furc-1 Klp61Furc-3 Klp61Furc-4 lethal | recessive | second instar larval stage Klp61FMk5 viable, with Klp61FUbi-p63E.T:Avic\GFP/Klp61F06836 Klp61F07012 viable (with Klp61F06836), with Klp61F07012 Klp61FUbi-p63E.T:Avic\GFP viable (with Klp61F07012) Klp61FUbi-p63E.T:Avic\GFP viable (with Klp61FUbi-p63E.T:Avic\GFP) Klp61F07012 viable (with Klp61FUbi-p63E.T:Avic\GFP), with Klp61F07012 Klp61F06836 Sterility Allele fertile, with Klp61FUbi-p63E.T:Avic\GFP/Klp61F06836 Klp61F07012 fertile (with Klp61F06836), with Klp61F07012 Klp61FUbi-p63E.T:Avic\GFP fertile (with Klp61F07012) Klp61FUbi-p63E.T:Avic\GFP fertile (with Klp61FUbi-p63E.T:Avic\GFP) Klp61F07012 fertile (with Klp61FUbi-p63E.T:Avic\GFP), with Klp61F07012 Klp61F06836 Other Phenotypes Allele cytokinesis defective Klp61F07012 mitotic cell cycle defective Klp61Furc-1 Klp61Furc-2 Klp61Furc-3 Klp61Furc-4 Klp61Fv27 mitotic cell cycle defective | embryonic stage 4 (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 mitotic cell cycle defective | embryonic stage 4 (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC mitotic cell cycle defective | larval stage Klp61F07012 mitotic cell cycle defective | pre-blastoderm stage Klp61F07012 mitotic cell cycle defective | pre-blastoderm stage (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 mitotic cell cycle defective | pre-blastoderm stage (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC mitotic cell cycle defective | recessive Klp61F06345 Klp61F06836 Klp61F07012 Klp61Furc-1 mitotic cell cycle defective | somatic clone | third instar larval stage Klp61FA9 Klp61FA67 Klp61FB14 Klp61FDL42 Klp61FH10 neuroanatomy defective | larval stage Klp61FGD14149 Phenotype manifest in Allele aster & neuroblast & larva Klp61F07012 aster & spermatocyte Klp61F07012 aster & spermatocyte (with Df(3L)bab-PG) Klp61F07012 aster & spermatocyte (with Klp61F06345) Klp61F07012 aster & spermatocyte (with Klp61F07012) Klp61F06345 astral microtubule Klp61FdsRNA.cGa centriole & spermatocyte Klp61F06345 Klp61F07012 centrosome & neuroblast & larva Klp61F07012 centrosome & spermatocyte Klp61F07012 centrosome & spermatocyte (with Df(3L)bab-PG) Klp61F07012 centrosome & spermatocyte (with Klp61F06345) Klp61F07012 centrosome & spermatocyte (with Klp61F07012) Klp61F06345 embryonic/larval brain Klp61F06345 Klp61F06836 Klp61F07012 Klp61Furc-1 Klp61Furc-3 Klp61Furc-4 embryonic/larval brain | somatic clone | third instar larval stage Klp61FA9 Klp61FA67 Klp61FB14 Klp61FH10 Klp61FDL42 embryonic/larval neuroblast Klp61F07012 embryonic/larval neuroblast | larval stage Klp61FGD14149 embryonic/larval neuroblast | somatic clone | third instar larval stage Klp61FA9 Klp61FA67 Klp61FB14 Klp61FH10 Klp61FDL42 ganglion mother cell | larval stage Klp61FGD14149 imaginal disc | larval stage Klp61F06345 Klp61F06836 Klp61F07012 Klp61Furc-1 Klp61Furc-3 Klp61Furc-4 larval brain & nuclear chromosome & larva Klp61Fv27 meiotic telophase II & spermatocyte Klp61F07012 microtubule organizing center | pre-blastoderm stage (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 microtubule organizing center | pre-blastoderm stage (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC mitosis & nuclear chromosome Klp61FdsRNA.cGa mitotic cell cycle & spermatocyte Klp61F07012 nuclear chromosome Klp61F06345 Klp61F06836 Klp61F07012 nuclear lamina & neuroblast & larva Klp61F07012 Klp61Furc-1 nuclear lamina & spermatocyte Klp61F07012 S2 cell-line Klp61FdsRNA.cGa spermatid (with Klp61F07012) Klp61Furc-1 spermatid (with Klp61Furc-1) Klp61F07012 spermatocyte (with Klp61F07012) Klp61Furc-1 spermatocyte (with Klp61Furc-1) Klp61F07012 spermatocyte fusome Klp61F06345 Klp61F07012 spindle Klp61F07012 Klp61FdsRNA.cGa spindle & neuroblast & larva Klp61F07012 spindle & spermatocyte Klp61F07012 spindle & spermatocyte (with Df(3L)bab-PG) Klp61F07012 spindle & spermatocyte (with Klp61F06345) Klp61F07012 spindle & spermatocyte (with Klp61F07012) Klp61F06345 Klp61Furc-1 spindle & spermatocyte (with Klp61Furc-1) Klp61F07012 spindle | embryonic stage, with Klp61FUbi-p63E.T:Avic\GFP/Klp61F06836 Klp61F07012 spindle | embryonic stage (with Klp61F06836), with Klp61F07012 Klp61FUbi-p63E.T:Avic\GFP spindle | embryonic stage (with Klp61F07012) Klp61FUbi-p63E.T:Avic\GFP spindle | embryonic stage (with Klp61FUbi-p63E.T:Avic\GFP) Klp61F07012 spindle | embryonic stage (with Klp61FUbi-p63E.T:Avic\GFP), with Klp61F07012 Klp61F06836 spindle | pre-blastoderm stage Klp61F07012 spindle microtubule Klp61FdsRNA.cGa spindle microtubule | embryonic stage 4 Klp61F07012 spindle microtubule | embryonic stage 4 (with Klp61F3YF.Ubi-p63E.T:Hsap\MYC) Klp61F07012 spindle microtubule | embryonic stage 4 (with Klp61F07012) Klp61F3YF.Ubi-p63E.T:Hsap\MYC spindle pole Klp61FdsRNA.cGa testis Klp61Furc-1
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of Klp61F Class Mutagen Stocks Known lesion Klp61F06345 hypomorphic allele - genetic evidence P-element activity 1 Yes Klp61F07012 hypomorphic allele - genetic evidence P-element activity 1 Yes Klp61Ff02870 piggyBac transposase 1 -- Klp61FMk2 ethyl methanesulfonate 1 -- Klp61FMk5 ethyl methanesulfonate 1 -- Klp61Furc-1 loss of function allele P-element activity 1 Yes Klp61F06836 hypomorphic allele - genetic evidence P-element activity 0 Yes Klp61F6D283 ethyl methanesulfonate 0 -- Klp61F7415 0 -- Klp61FA67 ethyl methanesulfonate 0 -- Klp61FA9 ethyl methanesulfonate 0 -- Klp61FB14 ethyl methanesulfonate 0 -- Klp61FDL42 ethyl methanesulfonate 0 -- Klp61FH10 ethyl methanesulfonate 0 -- Klp61Funspecified 0 -- Klp61Furc-2 ethyl methanesulfonate 0 -- Klp61Furc-3 ethyl methanesulfonate 0 -- Klp61Furc-4 ethyl methanesulfonate 0 -- Klp61Fv27 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Klp61F Class Mutagen Stocks Known lesion Klp61FGD14149 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Klp61FUbi-p63E.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes Klp61FGL00441 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Klp61FHMS00552 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Klp61FUbi-p63E.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Klp61F3YF.Ubi-p63E.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Klp61FdsRNA.cGa in vitro construct - RNAi 0 Yes Klp61FdsRNA.cLa in vitro construct - RNAi 0 Yes Klp61FKK100504 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Klp61FMtnA.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Klp61FNIG.9191R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Klp61FS1050A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FS1050A.S1054A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FS949A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT1045A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT1045A.S1050A.S1054A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT933A.MtnA.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes Klp61FT933A.Ubi-p63E.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Klp61FT933D.Ubi-p63E.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Klp61FT:SV5\V5 in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)4231 (Wei et al., 2003) Df(3L)Ar14-8 (Spradling et al., 1999, Wilson et al., 1997) Df(3L)bab-PG (Spradling et al., 1999, Wilson et al., 2004) Df(3L)BSC311 (Christensen et al., 2007.10.29) Df(3L)BSC363 (Christensen et al., 2007.10.29) Df(3L)BSC431 (Christensen et al., 2008.4.15) Df(3L)BSC799 (Christensen et al., 2009.5.6)
Not disrupted in	Df(3L)Ar12-1 (Wilson et al., 1997) Df(3L)B67 (Wei et al., 2003)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14149} NA P{GD17447} NA P{KK100504} NA P{NIG.9191R} NA P{TRiP.GL00441} NA P{TRiP.HMS00552} NA characterization construct P{KLP61F.H} NA P{Ubi-Klp61F.GFP.T933A} NA P{Ubi-Klp61F.GFP.T933D} NA P{Ubi-Klp61F.GFP} NA P{Ubi-Myc-Klp61F.3YF} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Klp61Furc-1 No P{lArB}Klp61Fv27 No P{PZ}Klp61F06345 No P{PZ}Klp61F06836 No P{PZ}Klp61F07012 No miscellaneous insertions PBac{WH}Klp61Ff02870 NA
Gene Ontology: Function, Process & Cellular Component ( 29 unique terms )
Terms Based on Experimental Evidence ( 23 terms )
Molecular Function
CV term Evidence References
motor activity inferred from direct assay (Cole and Scholey, 1995)
Biological Process
CV term Evidence References
cell cycle inferred from mutant phenotype (Kiger et al., 2003) centrosome duplication inferred from mutant phenotype (Müller et al., 2010) centrosome separation inferred from mutant phenotype (Gandhi et al., 2004) Golgi organization inferred from mutant phenotype (Bard et al., 2006) microtubule bundle formation inferred from direct assay (Tao et al., 2006) mitosis inferred from mutant phenotype (Ducat et al., 2008) mitotic centrosome separation inferred from genetic interaction with ncd AND inferred from mutant phenotype (Sharp et al., 1999) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle stabilization inferred from mutant phenotype (Garcia et al., 2009) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) plus-end directed microtubule sliding inferred from direct assay (Tao et al., 2006) protein homotetramerization inferred from direct assay (Tao et al., 2006) protein secretion inferred from mutant phenotype (Bard et al., 2006)
Cellular Component
CV term Evidence References
colocalizes_with centrosome inferred from direct assay (Cheerambathur et al., 2008) cytoplasm inferred from direct assay (Cole and Scholey, 1995) endoplasmic reticulum inferred from direct assay (Bard et al., 2006) fusome inferred from direct assay (Wilson, 2005) Golgi apparatus inferred from direct assay (Bard et al., 2006) kinesin complex inferred from direct assay (Cole and Scholey, 1995) microtubule inferred from direct assay (Cheerambathur et al., 2008) microtubule associated complex inferred from direct assay (Cole and Scholey, 1995, Hughes et al., 2008) spindle inferred from direct assay (Cheerambathur et al., 2008)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821 (FlyBase Curators et al., 2004-) microtubule binding inferred from electronic annotation with InterPro:IPR025901 (FlyBase Curators et al., 2004-) microtubule motor activity inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) plus-end-directed microtubule motor activity inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1995.9.1)
Biological Process
CV term Evidence References
microtubule-based movement inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) mitotic spindle organization non-traceable author statement (Swiss-Prot Project Members, 1995.9.1)
Cellular Component
CV term Evidence References
kinesin complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) plus-end kinesin complex inferred from sequence or structural similarity with Saccharomyces KIP1 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1995.9.1) spindle non-traceable author statement (Swiss-Prot Project Members, 1995.9.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Klp61F - Arf79F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - Bacc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - CG10984 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - CG14109 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - CG2540 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - CanA1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - Dip1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - ana2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - papi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - thoc7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp61F - vig anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Klp61F allele Gene References Klp61F06345 ncd (Wilson et al., 2004) Klp61F07012 ncd (Wilson et al., 2004) Klp61Furc-1 ncd (Wilson et al., 2004) unspecified Klp67A (Gandhi et al., 2004) ncd (Sharp et al., 1999) Wk (Furman and Omel'ianchuk, 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63681 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004378 DroID - A comprehensive database of gene and protein interactions. • FBgn0004378 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38135
Orthologs
OrthoDB Orthologs (48) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6QRHPC Dipteran inclusive ortholog search EOG6KKZB3 Insect inclusive ortholog search EOG608M4D Arthropod inclusive ortholog search EOG67H45D Metazoa inclusive ortholog search EOG6W71PD
Orthologs in Drosophila Species (EOG6QRHPC)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Klp61F     Drosophila simulans   Dsim\GD13489 Y   Drosophila sechellia   Dsec\GM14227 Y   Drosophila erecta   Dere\GG14613 Y   Drosophila yakuba   Dyak\GE20974 Y   Drosophila ananassae   Dana\GF10068 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21600 Y   Drosophila persimilis   Dper\GL16080 Y   Drosophila willistoni   Dwil\GK11822 Y   Drosophila virilis   Dvir\GJ13211 Y   Drosophila mojavensis   Dmoj\GI12274 Y   Drosophila grimshawi   Dgri\GH16010 Y
Orthologs in non-Drosophila Dipterans (EOG6KKZB3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014084   Anopheles gambiae Malaria mosquito  Agam\AGAP007502   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008622
Orthologs in non-Dipteran Insects (EOG608M4D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB46971   Apis mellifera Western honey bee  Amel\GB47488   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012292   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20572   Atta cephalotes Leafcutter ant  Acep\ACEP26453   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12596   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23124   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21488   Linepithema humile Argentine ant  Lhum\LH23607   Linepithema humile Argentine ant  Lhum\LH11266   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21862   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14475   Solenopsis invicta Red fire ant  Sinv\SINV21327   Solenopsis invicta Red fire ant  Sinv\SINV25485   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002268   Bombyx mori Silkmoth  Bmor\BGIBMGA008726   Pediculus humanus Human body louse  Phum\PHUM434560   Tribolium castaneum Red flour beetle  Tcas\TC004751   Tribolium castaneum Red flour beetle  Tcas\TC004752   Tribolium castaneum Red flour beetle  Tcas\TC014244
Orthologs in non-Insect Arthropods (EOG67H45D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\TMEM45A   Daphnia pulex Water flea  Dpux\ApoB   Daphnia pulex Water flea  Dpux\JGI_V11_57725   Ixodes scapularis Deer tick  Isca\ISCW006455
Orthologs in non-Arthropod Metazoa (EOG6W71PD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\bmk-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020414gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026503gn   Danio rerio Zebrafish  Drer\kif11   Xenopus tropicalis Western clawed frog  Xtro\kif11   Gallus gallus Domestic chicken  Ggal\KIF11   Mus musculus House mouse  Mmus\Kif11   Rattus norvegicus Norway rat  Rnor\Kif11   Homo sapiens Human  Hsap\KIF11
Human Orthologs (1)
Gene OMIM HGNC Hsap\KIF11
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13489 Drosophila sechellia Dsec\GM14227 Drosophila erecta Dere\GG14613 Drosophila yakuba Dyak\GE20974 Drosophila ananassae Dana\GF10068 Drosophila pseudoobscura pseudoobscura Dpse\GA21600 Drosophila persimilis Dper\GL16080 Drosophila willistoni Dwil\GK11822 Drosophila virilis Dvir\GJ13211 Drosophila mojavensis Dmoj\GI12274 Drosophila grimshawi Dgri\GH16010
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	35509 w*; P{Ubi-Klp61F.GFP}3 35510 w*; P{Ubi-Klp61F.GFP}2 35508 Klp61Furc-1/TM6B, Tb1 32011 w*; P{KLP61F.H}3/TM3, Sb1 32012 P{PZ}Klp61F06345/TM6B, Tb1 11710 P{PZ}Klp61F07012 ry506/TM6B, Tb1 35804 y1 sc* v1; P{TRiP.GL00441}attP40 33685 y1 sc* v1; P{TRiP.HMS00552}attP2/TM3, Sb1 32013 mwh1 Klp61FMk5 rhove-1 red1 e*/TM6B, Tb1
Harvard	- PBac{WH}Klp61Ff02870
VDRC	v52548 w1118; P{GD14149}v52548 v52549 w1118; P{GD14149}v52549
	More stocks available...
Genomic Clones ( 1 )
	BACR17K17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 209 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD15641
Other clones	DPiM_LD15641 RT07324
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT18509 AT27717 GM09567 GM12362 GM13005 GM13405 LD04280 LD07059 LD10676 LD10984 LD11639 LD12480 LD12969 LD13384 LD14571 LD18121 LD18462 LD19008 LD21891 LD22078 LD22393 LD23022 LD25828 LD27186 LD30833 LD30871 LD31634 LD34033 LD34739 LD35311 LD36993 LD38277 LD40531 LD41672 LD43317 LD44922 LD45930 RE30012 RE31477 RE52968 RE72066 SD01713 SD03396 SD04243 SD08410 SD13543 SD20629
Other clones	12550 35283 60307 67492 73334 82427 106650 119580 174496 191732 214383.30 214567.25 524034 CK01.65B.G6 Dmel_dig_018049_0622_0864 EK053159 EK113520 EK146421 EK175134 EK195522 EK202534 EK239801 EK243127 EK243130 EK244244 EK258101 EK275612 EK279319 EK294222 EP09019 GM23817 RP003006101 RP003009330 RP003011933 RP003017837 RP003021049 RP003021440 RP003021505 RP003028562 RP003028670 RP003029989 RP003034081 RP003035229 RP003035712 RP003045429 RP003056741 RP003062463 RP003063792 RP003064831 RP003067191 RP003071085 RP003083297 RP003088977 RP003093940 RP003094310 RP003095529 RP003095934 RP003102736 RP003118198 RP003124115 RP003129626 RP003132374 RP003152866 RP003158455 RP003161489 RP003162692 RP003164816 RP003172510 RP003174042 RP003174805 RP003178629 RP003184507 RP003187977 RP003189508 RP003196386 RP003201870 RP003204732 RP003205618 RP003210239 RP003211806 RP003215851 RP003225446 RP003226634 RP003234528 RP003248537 RP003248998 RP003253432 RP003254280 RP003256955 RP003257203 RP003258394 RP003260593 RP003261028 RP003262747 RP003268578 RP003271117 RP003273429 RP003274368 RP003274666 RP003282773 RP003294320 RP003296268 RP003309839 RP003313276 RP003337038 RP003341948 RP003342838 RP003344716 RP003348678 RP003351190 RP003360832 RP003363927 RP003367220 RP003370641 RP003378568 RP003385453 RP003396235 RP003396553 RP003401179 RP003412543 RP003416400 RP003425593 RP003430143 RP003432505 RP003446568 RP003446608 RP003449162 RP003451730 RP003462901 RP003469121 RP003469235 RP003485772 RP003490759 RP003492557 RP003494629 RP003506343 RP003514825 RP003517800 RP003527797 RP003533041 RP003535442 RP003542686 RP003546372 RP003556550 RP003556915 RP003556998 RP003568238 RP003569091 RP003570357 RP003581517 RP003581926 RP003613559 RP003613954 RP003615697 RP003625314 RP003628611 RP003637468 RP003656758 RP003675623
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004378 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38135
Antibody Information
	polyclonal (Sharp et al., 1999, Barton et al., 1995, Brust-Mascher et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Klp61F Krp130 (Scholey, 1999.5.5)
Additional comments	CG32318 appears to be a chimeric gene, with the 5' part of the gene derived from Klp61F and the 3' part of the gene derived from Psf1. (Rogers and Hartl, 2012) "CG32318" is a putative chimeric gene derived from the "Klp61F" and "Psf1" genes (where coding sequences of the two parental genes contribute to the coding sequence of the chimeric gene). (Rogers et al., 2009)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) dsRNA directed against Klp61F inhibits protein secretion in S2 cells and causes aggregation and swelling of the Golgi membranes in more than 50% of the treated cells. (Bard et al., 2006) dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion. (Bard et al., 2006) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: during mitosis, centrosome separation is prevented, resulting in monopolar spindles. (Gandhi et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Spindle pole movements in embryos are directed by a temporally coordinated balance of forces generated by three mitotic motors; cytoplasmic dynein, Klp61F and ncd. Dynein acts to move the poles apart throughout mitosis, and this activity is augmented by Klp61F after the fenestration of the nuclear envelope, which occurs at the onset of prometaphase. ncd generates forces that pull the poles together between interphase and metaphase, antagonising the activity of both cytoplasmic dynein and Klp61F and serving as a brake for spindle assembly. (Sharp et al., 2000) Klp61F cycles between spindles during mitosis and fusomes during interphase. (Wilson, 1999) Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland. (Harvie et al., 1998) Klp61F is involved in the control of cell division. (Omel'yanchuk et al., 1997) Klp61F protein, a homotetrameric BimC-related kinesin, consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends. Such a bipolar 'minifilament' could crosslink spindle microtubules and slide them relative to one another. (Kashina et al., 1996) Antibody staining reveals multiple bimC family members are expressed in Drosophila. Klp61F is an essential gene and biochemical and functional analysis of the protein demonstrates it is not functionally redundant for centrosome separation during neuroblast separation. (Barton et al., 1995) Relationship to cloned Klp genes not known. Purified Klp61F behaves as a homotetrameric complex of four subunits that moves toward the plus ends of microtubule tracts at approximately 0.04μm/s. (Cole and Scholey, 1995) Encodes a 130kD kinesin related protein that behaves as a homotetramer and moves towards the plus ends of microtubules. (Cole and Scholey, 1995) Identified as a 130kD polypeptide in an ATP MAPs 1-24 hour embryonic fraction. A 490kD homotetrameric complex consisting of 4 130kD subunits display a 'slow' plus-end directed motor activity capable of moving single microtubules. (Cole et al., 1994) The sequence of the Klp61F protein has been compared with the sequences of a variety of kinesin family proteins. (Goodson et al., 1994) Klp61F has been cloned, partially sequenced and its expression pattern analysed. (Stewart et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057470 RefSeq (Proteins) • NP_476818 DDBJ / EMBL / GenBank • AA440767 AAA03718 AAA28655 AAF47458 AAL39587 ABG91086 ABG91087 AC004489 AC005847 AQ026225 AW942371 AY069442 AY729990 CZ485426 DQ782381 DQ782382 M74428 U01842 Entrez Gene - A searchable database of RefSeq genes. • 38135 UniProtKB/Swiss-Prot • P46863 UniProtKB/TrEMBL • Q0PQ30 Q0PQ31
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Kinesin-associated microtubule-binding domain (IPR025901) Kinesin, motor domain (IPR001752) Kinesin, motor region, conserved site (IPR019821) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD15641
Linkouts
	BioGRID - A database of protein and genetic interactions • 63681 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004378 DroID - A comprehensive database of gene and protein interactions. • FBgn0004378 DRSC - Results from RNAi screens. • FBgn0004378 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004378 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9191-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004378 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38135 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/klp61f.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38135 modMine - Data generated by the modENCODE project. • FBgn0004378
Synonyms & Secondary IDs ( 34 )
Reported As
Symbol Synonym	6345 (Harvie et al., 1998) CG9191 (Goldstein and Gunawardena, 2000, Rogers and Hartl, 2012, Goshima et al., 2007, Ducat et al., 2008, Hughes et al., 2008, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) CG9191-PA (Rogers et al., 2009) DmKLP61F (Gadde and Heald, 2004, Matthies et al., 2001, Hirokawa et al., 1998) DmKlp61F (Kim and Endow, 2000, Moore and Endow, 1996, Dagenbach and Endow, 2004, Hirokawa, 2012) DmKRP130 (Moore and Endow, 1996) KIF 8 (Miki et al., 2001) KIF11 (Hirokawa, 2012.11.20) KLP2 (Wilson et al., 1992, Stewart et al., 1991) Klp2   KLP61 (Gonzalez et al., 1998) KLP61f (Kai et al., 2005) klp61f (Wilson et al., 2004, Wilson et al., 2004.8.21, Cheerambathur et al., 2008) KLP61F (Kwon and Scholey, 2004, Adams et al., 2001, Wilson, 1999, Wilson, 1999, Glover et al., 1998, Compton, 1998, Kashina et al., 1997, Wilson and Borisy, 1996, Kashina et al., 1996, Barton et al., 1994, Heck et al., 1993, Wilson, 2005, Silverman-Gavrila and Wilde, 2006, Tao et al., 2006, Lawrence et al., 2002, Bodey et al., 2009, Cheerambathur et al., 2008, Wilson et al., 2005, van den Wildenberg et al., 2008, Brust-Mascher et al., 2009, Vendra et al., 2007, Civelekoglu-Scholey et al., 2010, Sommi et al., 2010, Tao and Scholey, 2010) Klp61F (De Lucia et al., 2005, Cane et al., 2013, Christensen et al., 2007.10.29, Christensen et al., 2007.10.29, Goshima and Vale, 2005, Goshima et al., 2007, Christensen et al., 2008.4.15, Kim et al., 2007, Georlette et al., 2007, Somma et al., 2008, Lindquist et al., 2011, Rogers et al., 2009, Ducat et al., 2008, Christensen et al., 2009.5.6, Slack et al., 2006, Vale et al., 2009, Wei et al., 2003, Anholt and Williams, 2010, Goldstein, 2010.9.10, Lakämper et al., 2010, Liu et al., 2011, Neumüller et al., 2011, Loop et al., 2004, Fisher et al., 2008, Japanese National Institute of Genetics, 2012.5.21, Kalajdzic et al., 2012, Miki et al., 2005, Hirokawa, 2012.11.20) klp61F (Gaspar and Szabad, 2009, Garcia et al., 2009, Mottier-Pavie et al., 2011) Klp61f (Casper and Van Doren, 2009) Klp 61F (Miki et al., 2001) Klp61F/KRP130 (Endow, 1999) KRP130 (Kashina et al., 1997) Krp130   KRP130 (Cole and Scholey, 1995, Kashina et al., 1996, Kashina et al., 1995, Cole et al., 1994, Cole et al., 1994) KRP(130) (Cole and Scholey, 1995) l(3)6D283 (Wei et al., 2003) l(3)07012   l(3)Klp61F (Wei et al., 2003) urc   urcn (Wilson and Borisy, 1995, Wilson and Fuller, 1993)
Name Synonym	kinesin-5 (Cheerambathur et al., 2008, Garcia et al., 2009, Civelekoglu-Scholey et al., 2010, Tao and Scholey, 2010) kinesin-5 motor (Vale et al., 2009) kinesin-like protein (Goldstein, 1993.9.15) Kinesin-like protein at 61F (Hirokawa, 2012.11.20) Kinesin-related protein-130kD (Cole and Scholey, 1995) urchin (Wilson, 1994, Wilson and Fuller, 1991, Wilson and Borisy, 1995, Goodson et al., 1994, Wilson et al., 1992, )
Secondary FlyBase IDs
	FBgn0004923 FBgn0010911 FBgn0015744
References ( 158 )
Generate a list of	All references relating to this gene ( 158 )
List References by type	Research paper  ( 70 ) Supplementary material  ( 10 ) Review  ( 26 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 23 ) Abstract  ( 16 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Letter  ( 1 ) Stock list  ( 1 ) Website  ( 2 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 8 )
	Cane et al., 2013, J. Cell Biol. 200(2): 203--218 Elevated polar ejection forces stabilize kinetochore-microtubule attachments. [FBrf0220555] Kalajdzic et al., 2012, PLoS ONE 7(6): e40296 Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms. [FBrf0218826] Rogers and Hartl, 2012, Mol. Biol. Evol. 29(2): 517--529 Chimeric Genes as a Source of Rapid Evolution in Drosophila melanogaster. [FBrf0217452] Ambegaokar and Jackson, 2011, Hum. Mol. Genet. 20(24): 4947--4977 Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation. [FBrf0217690] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Liu et al., 2011, J. Biol. Chem. 286(8): 6201--6210 Loop 5-directed Compounds Inhibit Chimeric Kinesin-5 Motors: IMPLICATIONS FOR CONSERVED ALLOSTERIC MECHANISMS. [FBrf0213083] Mottier-Pavie et al., 2011, J. Cell Sci. 124(5): 706--717 Phenotypic analysis of misato function reveals roles of noncentrosomal microtubules in Drosophila spindle formation. [FBrf0212996] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...