Gene model reviewed during 5.46
Gene model reviewed during 5.56
3.0, 2.8 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp67A using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Klp67A in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Klp67A CG10923
Source for merge of Klp67A anon-WO0140519.224 anon-WO0172774.120 was sequence comparison ( date:051113 ).
S2 cells treated with RNAi against Klp10A show increased poleward flux rates of tubulin subunits in mitotic spindles and show suppressed α-tubulin turnover at the spindle equator but not at the poles. The chromatid-to-pole velocity during anaphase is increased in these cells. Pole-to-pole spindle lengths are significantly increased in the RNAi treated cells compared to controls.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and an aberrantly long spindle that is monastral and bipolar when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show an expansion of the metaphase spindle. Sometimes RNAi of this gene show centrosome detachment from the kinetochore microtubules.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: cells become arrested in metaphase and contain abnormally elongated, curved spindles which cause the entire cell to assume an elongated shape and exclude mitochondria from the cell poles. Additionally, metaphase chromosomes show an abnormal configuration.
The majority of post-meiotic Klp67A mutant spermatids contain two nuclei, which vary in size. During meiosis I, mutant primary spermatocytes have an increased number of microtubules throughout the cell and lack a distinct central spindle. Additionally, the k-fibres that link the chromosomes to the spindle are bent and wavy, instead of being straight, as in wild type.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Klp67A is a mitotic motor that may have a unique role of positioning mitochondria near the spindle.