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Gene Dmel\Klp67A

General Information
Symbol	Dmel\Klp67A	Species	D. melanogaster
Name	Kinesin-like protein at 67A	Annotation symbol	CG10923
Feature type	protein_coding_gene	FlyBase ID	FBgn0004379
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	DmKlp67A, KLP3
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B2-67B2	Sequence location	3L:9,355,736..9,360,051 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Kinesin-like protein at 67A is referred to in FlyBase by the symbol Dmel\Klp67A (CG10923, FBgn0004379). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: motor activity. There is experimental evidence that it is involved in the biological process: sister chromatid biorientation; centrosome separation; male meiosis chromosome segregation; chromosome segregation; mitotic spindle organization; cytokinesis; spindle elongation; microtubule polymerization; spindle assembly; male meiotic chromosome movement towards spindle pole. 15 alleles are reported. The phenotypes of these alleles are annotated with: spindle microtubule; S2 cell-line; spindle pole; spindle; cytoplasmic microtubule; astral microtubule. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Kinesin, motor domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1631630_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Klp67A. Gene sequence location is 3L:9355736..9360051.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B2-67B2   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References 67B1-67B5   (determined by in situ hybridisation)   (Deak et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Klp67A for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Klp67A-RA FBtr0076499  NM_079268   2899   814 Klp67A-RB FBtr0333886  NM_001274695   2991   740
Additional Transcript Data & Comments
Reported size (kB)	3.0, 2.8 (northern blot) (Stewart et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Klp67A-PA   FBpp0076227   92.3   814   9.67     NP_523992   AAF50296 Klp67A-PB   FBpp0306018   84.4   740   8.74     NP_001261624   AGB94319
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Kinesin, motor domain (IPR001752) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264943   AA735387   AA941962   AE014296 AAF50296   AE014296 AGB94319   AI113659   AI518034   AQ072986   AX154875   AX260158   AY071433 AAL49055   BG638338   BG638499   BI167013   BI229799   BI232562   BI366470   BI366522   BI368072   CK605524   CK605525   CK659108   CO282501   CO296007   EC073539   EC226382   EL884513   EL884637   GH741744   GH747005   GH747441   GH765840   GH766558   M74429 AAA28656   U89264 AAB49460
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VSW5 Q00084 P91945
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic central brain surface glia   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic/larval lymph gland   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) gonad   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) lateral cord surface glial cell   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & larval stage & pupal stage     (Stewart et al., 1991) adult stage | female   ovary   (Stewart et al., 1991) adult stage | male   testis   (Stewart et al., 1991)
Additional Descriptive Data
	Klp67A transcripts are expressed throughout development. In adults, they are detected only in the gonads. (Stewart et al., 1991)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Pereira et al., 1997) colocalizes_with kinetochore inferred from direct assay (Wang et al., 2010) microtubule associated complex inferred from direct assay (Pereira et al., 1997, Hughes et al., 2008) nucleus inferred from direct assay (Savoian et al., 2004) colocalizes_with nucleus inferred from direct assay (Wang et al., 2010) colocalizes_with spindle microtubule inferred from direct assay (Wang et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    250.1 Larval Midgut    3.3 Larval Hindgut    10.6 Larval Malpighian Tubules    7 Larval Fat Body    38.2 Larval Salivary Gland    11.8 Larval Trachea    31.325 Larval Carcass    23.925 Adult Head    1.1 Adult Eye    2.55 Adult Brain    6.5 Adult Thoracic-Abdominal Ganglion    6.7 Adult Crop    4.3 Adult Midgut    3.2 Adult Hindgut    2.8 Adult Malpighian Tubules    6.9 Adult Fat Body    5.1 Adult Salivary Gland    8.3 Adult Heart    1.2 Adult VirginFemale Spermatheca    9.5 Adult InseminatedFemale Spermatheca    8.8 Adult Ovary    395.6 Adult Testis    225.8 Adult Male Accessory Gland    1.6 Adult Carcass    7.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1.1   NA  Eye    2.55   NA  Brain    6.5   250.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    6.7   NA  Crop    4.3   3.3  Midgut    3.2   no informative data  Hindgut    2.8   7  Malpighian Tubules    6.9   no informative data  Fat Body    5.1   no informative data  Salivary Gland    8.3   NA  Heart    1.2   31.325  Trachea    NA   NA  VirginFemale Spermatheca    9.5   NA  InseminatedFemale Spermatheca    8.8   NA  Ovary    395.6   NA  Testis    225.8   NA  Male Accessory Gland    1.6   no informative data  Carcass    7.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female    55 ovary, mated 4-day female    43 testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (27) central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female  (55) ovary, mated 4-day female  (43) testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female    55 ovary, mated 4-day female    43 testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female    55 ovary, mated 4-day female    43 testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female    55 ovary, mated 4-day female    43 testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  27 central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female  (55) ovary, mated 4-day female  (43) testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female    55 ovary, mated 4-day female    43 testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    16 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    5 carcass, larvae L3 wandering    8 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    1 ovary, virgin 4-day female    55 ovary, mated 4-day female    43 testis, mated 4-day male    24 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    52 embryo 04-06hr    72 embryo 06-08hr    63 embryo 08-10hr    43 embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (56) embryo 02-04hr  (52) embryo 04-06hr  (72) embryo 06-08hr  (63) embryo 08-10hr  (43) embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day  26 adult female 30day  (29) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    52 embryo 04-06hr    72 embryo 06-08hr    63 embryo 08-10hr    43 embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    52 embryo 04-06hr    72 embryo 06-08hr    63 embryo 08-10hr    43 embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day    26 adult female 30day    29 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    52 embryo 04-06hr    72 embryo 06-08hr    63 embryo 08-10hr    43 embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (56) embryo 02-04hr  (52) embryo 04-06hr  (72) embryo 06-08hr  (63) embryo 08-10hr  (43) embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day  26 adult female 30day  29 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    52 embryo 04-06hr    72 embryo 06-08hr    63 embryo 08-10hr    43 embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    52 embryo 04-06hr    72 embryo 06-08hr    63 embryo 08-10hr    43 embryo 10-12hr    17 embryo 12-14hr    11 embryo 14-16hr    5 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    21 white prepupae 24hr    21 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    16 adult male 30day    12 adult female 01day    11 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    35 Schneider line 2 Sg4    30 embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1    26 embryonic S3    37 leg disc CME L1    33 wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    30 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    31 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    28 ovary fGS/OSS    44 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (35) Schneider line 2 Sg4  (30) embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1  26 embryonic S3  (37) leg disc CME L1  (33) wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9  (35) wing disc ML-DmD16-c3  (33) wing disc ML-DmD21  (30) wing disc ML-DmD32    25 haltere disc ML-DmD17-c3  (37) eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5  (31) mixed discs ML-DmD4-c1  (34) CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2  (28) ovary fGS/OSS  (44) ovary OSC  (45) ovary OSS  (31) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    35 Schneider line 2 Sg4    30 embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1    26 embryonic S3    37 leg disc CME L1    33 wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    30 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    31 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    28 ovary fGS/OSS    44 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    35 Schneider line 2 Sg4    30 embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1    26 embryonic S3    37 leg disc CME L1    33 wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    30 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    31 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    28 ovary fGS/OSS    44 ovary OSC    45 ovary OSS    31 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    35 Schneider line 2 Sg4    30 embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1    26 embryonic S3    37 leg disc CME L1    33 wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    30 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    31 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    28 ovary fGS/OSS    44 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (35) Schneider line 2 Sg4  30 embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1  26 embryonic S3  (37) leg disc CME L1  (33) wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9  (35) wing disc ML-DmD16-c3  (33) wing disc ML-DmD21  30 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3  (37) eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5  31 mixed discs ML-DmD4-c1  (34) CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2  28 ovary fGS/OSS  (44) ovary OSC  (45) ovary OSS  31 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    35 Schneider line 2 Sg4    30 embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1    26 embryonic S3    37 leg disc CME L1    33 wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    30 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    31 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    28 ovary fGS/OSS    44 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    35 Schneider line 2 Sg4    30 embryonic 1182-4H    25 embryonic GM2    12 embryonic Kc167    5 embryonic S1    26 embryonic S3    37 leg disc CME L1    33 wing disc CME-W2    22 wing disc ML-DmD8    22 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    30 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    31 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    28 ovary fGS/OSS    44 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult  (30) Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (32) Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (27) Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult  30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  32 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  27 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    25 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • RE52076 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004379 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10923-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Klp67A Stages(s) 4-6 Stages(s) 7-8 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | recessive Klp67AS042705 Sterility Allele female semi-sterile (with Df(3L)29A6) Klp67A322b24 fertile (with Klp67A322b24), with Df(3L)29A6 Klp67AUbi-p63E.T:Avic\GFP fertile (with Klp67AUbi-p63E.T:Avic\GFP), with Df(3L)29A6 Klp67A322b24 male sterile (with Df(3L)29A6) Klp67A322b24 Other Phenotypes Allele cytokinesis defective | male (with Df(3L)29A6) Klp67A322b24 meiotic cell cycle defective | male Klp67AS042705 meiotic cell cycle defective | male, with Scer\GAL4[-]: Klp67AEP3516 meiotic cell cycle defective | male (with Df(3L)29A6) Klp67A322b24 mitotic cell cycle defective (with Df(3L)29A6) Klp67A322b24 Phenotype manifest in Allele astral microtubule Klp67AdsRNA.cGa centrosome | blastoderm stage | maternal effect (with Df(3L)29A6) Klp67A322b24 cytoplasmic microtubule Klp67AMtnA.NES.T:Avic\GFP meiosis I | male (with Df(3L)29A6) Klp67A322b24 meiosis II | male (with Df(3L)29A6) Klp67A322b24 meiotic anaphase II & condensed nuclear chromosome | male (with Df(3L)29A6) Klp67AS042705 meiotic anaphase II & condensed nuclear chromosome | male (with Df(3L)29A6), with Scer\GAL4[-]: Klp67AEP3516 mitosis & nuclear chromosome Klp67AdsRNA.cGa mitotic anaphase B | blastoderm stage | maternal effect (with Df(3L)29A6) Klp67A322b24 mitotic metaphase | blastoderm stage | maternal effect (with Df(3L)29A6) Klp67A322b24 mitotic prometaphase & nuclear chromosome | male (with Df(3L)29A6) Klp67AS042705 mitotic prometaphase & nuclear chromosome | male (with Df(3L)29A6), with Scer\GAL4[-]: Klp67AEP3516 S2 cell-line Klp67AdsRNA.cGa Klp67AMtnA.NES.T:Avic\GFP spermatid & Nebenkern (with Df(3L)29A6) Klp67A322b24 spermatid & nucleus (with Df(3L)29A6) Klp67A322b24 spermatocyte & aster | ectopic (with Df(3L)29A6) Klp67A322b24 spermatocyte & astral microtubule (with Df(3L)29A6) Klp67A322b24 spermatocyte & centrosome | supernumerary (with Df(3L)29A6) Klp67A322b24 spermatocyte & condensed nuclear chromosome (with Df(3L)29A6) Klp67A322b24 spermatocyte & contractile ring (with Df(3L)29A6) Klp67A322b24 spermatocyte & nuclear chromosome (with Df(3L)29A6) Klp67AS042705 spermatocyte & nuclear chromosome (with Df(3L)29A6), with Scer\GAL4[-]: Klp67AEP3516 spermatocyte & spindle | supernumerary (with Df(3L)29A6) Klp67A322b24 spindle Klp67AMtnA.T:Avic\GFP Klp67AdsRNA.cGa spindle | blastoderm stage | maternal effect (with Df(3L)29A6) Klp67A322b24 spindle | embryonic stage 4 (with Df(3L)29A6) Klp67A322b24 spindle microtubule Klp67AdsRNA.cGa spindle microtubule | blastoderm stage | maternal effect (with Df(3L)29A6) Klp67A322b24 spindle pole Klp67AdsRNA.cGa
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Klp67A Class Mutagen Stocks Known lesion Klp67A322b24 Delta2-3 1 Yes Klp67AEP3516 P-element activity 1 -- Klp67AG4868 P-element activity 1 -- Klp67ANP0917 0 -- Klp67AS042705 Delta2-3 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Klp67A Class Mutagen Stocks Known lesion Klp67AGD7127 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Klp67AGL00446 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Klp67AGLV21009 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Klp67AJF02701 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Klp67AUbi-p63E.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Klp67AdsRNA.cGa in vitro construct - RNAi 0 Yes Klp67AKK100785 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Klp67AMtnA.NES.T:Avic\GFP in vitro construct - coding region fusion 0 Yes Klp67AMtnA.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Klp67AUbi-p63E.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7127} NA P{KK100785} NA P{TRiP.GL00446} NA P{TRiP.GLV21009} NA P{TRiP.JF02701} NA characterization construct P{Ubi-Klp67A.GFP} NA P{Ubi-Klp67A.MYC} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Klp67A322b24 NA P{EP}Klp67AEP3516 NA P{EP}Klp67AG4868 NA insertion of enhancer trap binary system P{GawB}Klp67ANP0917 No insertion of enhancer trap P{lacW}Klp67AS042705 No
Gene Ontology: Function, Process & Cellular Component ( 20 unique terms )
Terms Based on Experimental Evidence ( 16 terms )
Molecular Function
CV term Evidence References
motor activity inferred from direct assay AND inferred from sequence or structural similarity (Pereira et al., 1997)
Biological Process
CV term Evidence References
centrosome separation inferred from mutant phenotype (Gandhi et al., 2004) chromosome segregation inferred from mutant phenotype (Gandhi et al., 2004, Wang et al., 2010) cytokinesis inferred from mutant phenotype (Gandhi et al., 2004) male meiosis chromosome segregation inferred from mutant phenotype (Savoian et al., 2004) male meiotic chromosome movement towards spindle pole inferred from mutant phenotype (Savoian et al., 2004) microtubule polymerization inferred from mutant phenotype (Gandhi et al., 2004) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) sister chromatid biorientation inferred from mutant phenotype (Savoian et al., 2004) spindle assembly inferred from mutant phenotype (Gandhi et al., 2004, Savoian et al., 2004) spindle elongation inferred from mutant phenotype (Wang et al., 2010)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Pereira et al., 1997) colocalizes_with kinetochore inferred from direct assay (Wang et al., 2010) microtubule associated complex inferred from direct assay (Pereira et al., 1997, Hughes et al., 2008) nucleus inferred from direct assay (Savoian et al., 2004) colocalizes_with nucleus inferred from direct assay (Wang et al., 2010) colocalizes_with spindle microtubule inferred from direct assay (Wang et al., 2010)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001752 (FlyBase Curators et al., 2004-) microtubule motor activity inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P53086 (FlyBase, 1992-)
Biological Process
CV term Evidence References
microtubule-based movement inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P53086 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
kinesin complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P53086 (FlyBase, 1992-) traceable author statement (Pereira et al., 1997)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Klp67A - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp67A - CG6724 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp67A - Ote anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Klp67A - eIF6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Klp67A allele Gene References unspecified chb (Goshima et al., 2005) Klp61F (Gandhi et al., 2004) msps (Goshima et al., 2005)
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004379 DroID - A comprehensive database of gene and protein interactions. • FBgn0004379 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39068
Orthologs
OrthoDB Orthologs (43) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6WDDX9 Dipteran inclusive ortholog search EOG6RBQSR Insect inclusive ortholog search EOG6JQ2SN Arthropod inclusive ortholog search EOG6NS1SG Metazoa inclusive ortholog search EOG64R5T0
Orthologs in Drosophila Species (EOG6WDDX9)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Klp67A     Drosophila simulans   Dsim\GD12936 Y   Drosophila sechellia   Dsec\GM24884 Y   Drosophila erecta   Dere\GG14050 Y   Drosophila yakuba   Dyak\GE21253 Y   Drosophila ananassae   Dana\GF23742 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10646 Y   Drosophila persimilis   Dper\GL22636 Y   Drosophila willistoni   Dwil\GK16568 Y   Drosophila virilis   Dvir\GJ13594 Y   Drosophila mojavensis   Dmoj\GI11399 Y   Drosophila grimshawi   Dgri\GH16987 Y
Orthologs in non-Drosophila Dipterans (EOG6RBQSR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL009227   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013401   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015012   Anopheles gambiae Malaria mosquito  Agam\AGAP006803   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004544
Orthologs in non-Dipteran Insects (EOG6JQ2SN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45830   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014906   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17521   Atta cephalotes Leafcutter ant  Acep\ACEP12948   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18209   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16689   Linepithema humile Argentine ant  Lhum\LH19284   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18317   Solenopsis invicta Red fire ant  Sinv\SINV21848   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001356   Bombyx mori Silkmoth  Bmor\BGIBMGA010931   Tribolium castaneum Red flour beetle  Tcas\TC002763
Orthologs in non-Insect Arthropods (EOG6NS1SG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\MT-CYB
Orthologs in non-Arthropod Metazoa (EOG64R5T0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022160gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024588gn   Danio rerio Zebrafish  Drer\CH211-12J24.1   Xenopus tropicalis Western clawed frog  Xtro\kif18b   Xenopus tropicalis Western clawed frog  Xtro\kif18a   Gallus gallus Domestic chicken  Ggal\KIF18A   Gallus gallus Domestic chicken  Ggal\KIF18B   Mus musculus House mouse  Mmus\Kif18a   Mus musculus House mouse  Mmus\Kif18b   Rattus norvegicus Norway rat  Rnor\Kif18b   Rattus norvegicus Norway rat  Rnor\Kif18a   Homo sapiens Human  Hsap\KIF18A   Homo sapiens Human  Hsap\ENSG00000186185
Human Orthologs (2)
Gene OMIM HGNC Hsap\KIF18A     Hsap\ENSG00000186185
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12936 Drosophila sechellia Dsec\GM24884 Drosophila erecta Dere\GG14050 Drosophila yakuba Dyak\GE21253 Drosophila ananassae Dana\GF23742 Drosophila pseudoobscura pseudoobscura Dpse\GA10646 Drosophila persimilis Dper\GL22636 Drosophila willistoni Dwil\GK16568 Drosophila virilis Dvir\GJ13594 Drosophila mojavensis Dmoj\GI11399 Drosophila grimshawi Dgri\GH16987
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	35511 P{Ubi-Klp67A.GFP}1, w* 27549 y1 v1; P{TRiP.JF02701}attP2 35507 w*; P{EP}Klp67A322b24/TM6B, Tb1 35606 y1 sc* v1; P{TRiP.GL00446}attP40 36268 y1 sc* v1; P{TRiP.GLV21009}attP2 17269 w1118; P{EP}Klp67AEP3516/TM6B, Tb1 27170 y1 w*; P{EP}Klp67AG4868/TM3, Sb1 Ser1
VDRC	v51703 w1118 P{GD7127}v51703 v52105 w1118; P{GD7127}v52105
Genomic Clones ( 2 )
	BACR01E20 BACR48N08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE52076
Other clones	DPiM_RE52076
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH10062 LD08653 LD21225 LD37437 LP16615 RE07280 RE27657 RE28647 RE52007 RE53982
Other clones	44880 147706 EK160617 EK212507 IP15794 IP15894 LD22887 LD27326 RP001006089 RP001019429 RP001038351 RP001040089 RP001045564 UT01992
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004379 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39068
Antibody Information
	polyclonal (Wang et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Klp67A CG10923 (Milshina, 1999.11)
Source for database merge of	Source for merge of: Klp67A anon-WO0140519.224 anon-WO0172774.120 (FlyBase, 1996-) Source for merge of: Klp67A l(3)S042705 (Savoian et al., 2004)
Additional comments	Source for merge of Klp67A anon-WO0140519.224 anon-WO0172774.120 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	S2 cells treated with RNAi against Klp10A show increased poleward flux rates of tubulin subunits in mitotic spindles and show suppressed α-tubulin turnover at the spindle equator but not at the poles. The chromatid-to-pole velocity during anaphase is increased in these cells. Pole-to-pole spindle lengths are significantly increased in the RNAi treated cells compared to controls. (Buster et al., 2007) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and an aberrantly long spindle that is monastral and bipolar when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show an expansion of the metaphase spindle. Sometimes RNAi of this gene show centrosome detachment from the kinetochore microtubules. (Goshima et al., 2005) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: cells become arrested in metaphase and contain abnormally elongated, curved spindles which cause the entire cell to assume an elongated shape and exclude mitochondria from the cell poles. Additionally, metaphase chromosomes show an abnormal configuration. (Gandhi et al., 2004) The majority of post-meiotic Klp67A mutant spermatids contain two nuclei, which vary in size. During meiosis I, mutant primary spermatocytes have an increased number of microtubules throughout the cell and lack a distinct central spindle. Additionally, the k-fibres that link the chromosomes to the spindle are bent and wavy, instead of being straight, as in wild type. (Savoian et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Klp67A is a mitotic motor that may have a unique role of positioning mitochondria near the spindle. (Pereira et al., 1997) Klp67A has been cloned, partially sequenced and its expression pattern analysed. (Stewart et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079268 NM_001274695 RefSeq (Proteins) • NP_523992 NP_001261624 DDBJ / EMBL / GenBank • AAA28656 AAB49460 AAF50296 AAL49055 AGB94319 AQ072986 AX154875 AX260158 AY071433 BI366522 M74429 U89264 Entrez Gene - A searchable database of RefSeq genes. • 39068 UniProtKB/TrEMBL • P91945 Q9VSW5 Q00084
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Kinesin, motor domain (IPR001752) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE52076
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004379 DroID - A comprehensive database of gene and protein interactions. • FBgn0004379 DRSC - Results from RNAi screens. • FBgn0004379 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004379 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10923-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004379 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39068 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39068 modMine - Data generated by the modENCODE project. • FBgn0004379
Synonyms & Secondary IDs ( 21 )
Reported As
Symbol Synonym	0427/05 (Deak et al., 1997) anon-WO0140519.224   anon-WO0172774.120   CG10923 (Goldstein and Gunawardena, 2000, Goshima et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Hughes et al., 2008, Ni et al., 2011.7.19) DmKlp67A (Dagenbach and Endow, 2004, Dagenbach and Endow, 2004, Hirokawa, 2012) KIF 18A (Miki et al., 2001) KIF18A/B (Hirokawa, 2012.11.20) Kin-8 (Pandey et al., 2007) KLP3 (Stewart et al., 1991, Pereira et al., 1991, ) Klp3   Klp67 (Damasco et al., 2011) KLP67A (Gandhi et al., 2004, Kwon and Scholey, 2004, Hirokawa et al., 1998, Pereira and Karr, 1998, Pereira et al., 1997, Lawrence et al., 2002, Fernandez et al., 2009, Buster et al., 2007, Vendra et al., 2007, Miki et al., 2005) Klp67A (Goshima and Vale, 2005, Goshima et al., 2007, Savoian and Glover, 2010, D'Avino et al., 2005, Georlette et al., 2007, Somma et al., 2008, Gatt et al., 2005, Pandey et al., 2007, Laycock et al., 2006, Fisher et al., 2008, Korenjak et al., 2012, Miki et al., 2001, Hirokawa, 2012.11.20) klp67A (Wang et al., 2010) KRP67A (Lane and Allan, 1998) l(3)S042705
Name Synonym	kinesin-8 (Fernandez et al., 2009) Kinesin 8 (Pandey et al., 2007) kinesin like protein 67a (Pereira, 1997.2.10) Kinesin-like protein at 67A (Hirokawa, 2012.11.20) lethal (3) S042705
Secondary FlyBase IDs
	FBgn0044647 FBgn0046473 FBgn0060914
References ( 68 )
Generate a list of	All references relating to this gene ( 68 )
List References by type	Research paper  ( 28 ) Supplementary material  ( 5 ) Review  ( 10 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 4 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Website  ( 2 ) Curated genome annotation  ( 2 ) Patent  ( 2 )
Recent research papers ( 2 )
	Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Damasco et al., 2011, PLoS ONE 6(2): e14737 A signature inferred from Drosophila mitotic genes predicts survival of breast cancer patients. [FBrf0213204] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...