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Gene Dmel\pdm2

General Information
Symbol	Dmel\pdm2	Species	D. melanogaster
Name	POU domain protein 2	Annotation symbol	CG12287
Feature type	protein_coding_gene	FlyBase ID	FBgn0004394
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	pdm-2, pdm, miti, dPOU28, dPOU28/pdm-2, dPOU-28
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	33F2-33F3	Sequence location	2L:12,657,165..12,686,306 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene POU domain protein 2 is referred to in FlyBase by the symbol Dmel\pdm2 (CG12287, FBgn0004394). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; DNA binding. There is experimental evidence that it is involved in the biological process: central nervous system development; ectoderm development; regulation of transcription, DNA-dependent; neuroblast development. 21 alleles are reported. The phenotypes of these alleles are annotated with: multicellular structure; anatomical structure; embryonic/larval neuron; organ system; larval abdominal segment; neuroblast NB4-2; abdominal segment 2; larval abdominal segment 3; ventral nerve cord primordium; ganglion mother cell GMC4-2a; larval head; portion of tissue. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Homeobox, conserved site; Homeodomain; Homeodomain-like; Lambda repressor-like, DNA-binding domain; POU domain; POU-specific. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1624491_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of pdm2. Gene sequence location is 2L:12657829..12686313.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Polypeptides pdm2-PC pdm2-PD Transcripts pdm2-RD pdm2-RC
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 33F2-33F3   Limits computationally determined from genome sequence between P{lacW}bunk00612&P{EP}EP682EP682 and P{lacW}l(2)k07015k07015&P{PZ}l(2)rK639rK639
Experimentally Determined Cytological Location
Cytogenetic map Notes References 33F-33F   (determined by in situ hybridisation)   (Prakash et al., 1992) 33F-33F   (determined by in situ hybridisation)   (Dick et al., 1991) 33F1-33F2   (determined by in situ hybridisation)   (Lloyd and Sakonju, 1991) 33F-33F   (determined by in situ hybridisation)   (Billin et al., 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\pdm2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.51 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pdm2-RA FBtr0080393  NM_078834   2171   498 pdm2-RB FBtr0080392  NM_165017   3627   893 pdm2-RC FBtr0340539     3729   893 pdm2-RD FBtr0340540     4286   893
Additional Transcript Data & Comments
Reported size (kB)	2.5 (northern blot) (Billin et al., 1991) 2.3 (northern blot) (Prakash et al., 1992) 2.2 (northern blot) (Lloyd and Sakonju, 1991, Dick et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein pdm2-PA   FBpp0079974   55.5   498   6.52     NP_523558   AAF53209 pdm2-PB   FBpp0079973   98.5   893   6.90     NP_723763   AAF53208 pdm2-PC   FBpp0309438   98.5   893   6.90       pdm2-PD   FBpp0309439   98.5   893   6.90
Additional Polypeptide Data & Comments
Reported size (kDa)	498 (aa); 55 (kD) (Lloyd and Sakonju, 1991) 498 (aa); 54 (kD) (Prakash et al., 1992) 475 (aa); 53 (kD) (Dick et al., 1991) 413 (aa); 45.8 (kD) (Billin et al., 1991)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Homeobox, conserved site (IPR017970) POU-specific (IPR000327) Homeodomain (IPR001356) Homeodomain-like (IPR009057) Lambda repressor-like, DNA-binding domain (IPR010982) POU domain (IPR013847)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140737   AE014134 AAF53208   AE014134 AAF53209   BI238172   BI364340   BI365925   BT010239 AAQ23557   BT010257 AAQ23575   BT082108   BT132934   CO181887   CO268792   CO310066   CO333193   CO342174   CZ487765   EC202022   EC208572   EC213375   EC237767   EC239840   EC260469   GH740415   M65016 AAA28481   M81958 AAA28830   M93149 AAA28732   S80559 AAB21408
UniProtKB/Swiss-Prot	Q9VK71 P31369
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435094 2L:12,682,650..12,683,649 evidence=Reporter construct (in vivo) (Rouault et al., 2010, Halfon, 2012.6.19) regulatory region FBsf0000435287 2L:12,678,898..12,680,520 evidence=Reporter construct (in vivo) (Schroeder et al., 2004, Halfon, 2012.6.19) regulatory region FBsf0000435866 2L:12,674,735..12,676,045 evidence=Reporter construct (in vivo) (Berman et al., 2004, Halfon, 2012.6.19) regulatory region FBsf0000436134 2L:12,680,917..12,682,449 evidence=Reporter construct (in vivo) (Schroeder et al., 2004, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage | mid -- embryonic stage | late   presumptive embryonic/larval central nervous system   (Billin et al., 1991) embryonic stage   ectoderm | striped   (Dick et al., 1991) optic lobe   (Dick et al., 1991) labral segment   (Dick et al., 1991) central nervous system | restricted   (Dick et al., 1991) embryonic/larval labral segment sense organ   (Dick et al., 1991) embryonic stage | late   embryonic/larval labral segment sense organ | precursor   (Lloyd and Sakonju, 1991) abdominal dorsal multidendritic neuron   (Lloyd and Sakonju, 1991) presumptive embryonic/larval peripheral nervous system | restricted   (Lloyd and Sakonju, 1991) sensory neuron of embryonic/larval foregut   (Lloyd and Sakonju, 1991) scolopidial ligament cell of lateral abdominal cluster of abdominal segment 1 -- abdominal segment 7   (Lloyd and Sakonju, 1991) embryonic stage | mid   neuroblast | restricted   (Billin et al., 1991) embryonic stage 5   parasegment 7   (Lloyd and Sakonju, 1991) organism | 20-40% egg length   (Prakash et al., 1992) gastrula stage   ectoderm | striped   (Billin et al., 1991) organism | segment polarity expression pattern   (Prakash et al., 1992) embryonic stage 6   organism | odd pair rule expression pattern   (Lloyd and Sakonju, 1991) embryonic stage 10   tracheal primordium   (Lloyd and Sakonju, 1991) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage | mid -- embryonic stage | late   central nervous system | restricted   (Lloyd and Sakonju, 1991) embryonic stage | early   organism | 20-50% egg length   (Billin et al., 1991) Comment:25-50% egg length embryonic stage | mid   embryonic brain | restricted   (Billin et al., 1991) embryonic/larval hindgut   (Billin et al., 1991) embryonic stage | late   abdominal 1 dorsal bipolar neuron dbp -- abdominal 7 dorsal bipolar neuron dbp   (Lloyd and Sakonju, 1991) abdominal segment 8 -- abdominal segment 9 of peripheral nervous system   (Lloyd and Sakonju, 1991) chordotonal organ of lateral thoracic cluster of prothoracic segment   (Lloyd and Sakonju, 1991) dorsal thoracic bd1   (Lloyd and Sakonju, 1991) embryonic/larval neuron   (Lloyd and Sakonju, 1991) hypophysis   (Lloyd and Sakonju, 1991) embryonic stage   embryonic brain   (Dick et al., 1991) presumptive embryonic/larval peripheral nervous system | restricted   (Dick et al., 1991) hypophysis   (Dick et al., 1991) neurogenic region   (Dick et al., 1991) presumptive embryonic/larval central nervous system | restricted   (Prakash et al., 1992) embryonic stage 4 -- 6   central brain anlage in statu nascendi   (Fisher et al., 2012) dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi organism | gap expression pattern   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 5   organism | 90-100% egg length   (Prakash et al., 1992) Comment:95-100% egg length labral segment   (Lloyd and Sakonju, 1991) parasegment 7   (Lloyd and Sakonju, 1991) organism | 30-50% egg length   (Dick et al., 1991) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal ectoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic stage 9   organism | segment polarity expression pattern   (Lloyd and Sakonju, 1991) embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium anterior endoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) labial sensory complex primordium   (Fisher et al., 2012) Comment:reported as labial complex specific anlage maxillary sensory complex primordium   (Fisher et al., 2012) Comment:reported as maxillary organ specific anlage embryonic stage 10 -- 12   embryonic ganglion mother cell | segmentally repeated & subset   (Berman et al., 2004) embryonic neuroblast | segmentally repeated & subset   (Berman et al., 2004) embryonic stage 11 -- 12   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium anterior midgut primordium   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) dorsal sensory complex primordium   (Fisher et al., 2012) embryonic optic lobe primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) proventriculus primordium   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) ventral sensory complex anlage   (Fisher et al., 2012) embryonic ganglion mother cell   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) labial sensory complex primordium   (Fisher et al., 2012) maxillary sensory complex primordium   (Fisher et al., 2012) tracheal primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic brain   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic proventriculus   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012) embryonic foregut sensory structure   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012)
Additional Descriptive Data
	pdm2 transcripts are first expressed in the cellular blastoderm stage in two broad stripes in the posterior half of the embryo. These develop into a complicated pattern of stripes. Expression is later seen along the neurectoderm and in a restricted set of cells of the CNS and PNS. Expression in the head region occurs in the optic lobe primordium, brain primordia, clypeolabrum, epiphysis, labral sense organ, and in the ventral ectodermal regions of the labial, maxillary, and mandibullary buds. pdm2 transcripts are present at maximal levels in 0-12 hr embryos and at much reduced levels at later stages. (Dick et al., 1991) pdm2 transcripts are expressed at high levels during the first 12 hours of embryogenesis and in 2nd instar larvae and are detectable in other stages at reduced levels. pdm2 expression is initially seen at the cellular blastoderm stage in two bands in parasegments 7 and 11. During gastrulation, expression is seen in an odd pair-rule pattern which later becomes a segment polarity pattern with alternating wide and narrow stripes. At stage 10, expression is seen in the neurogenic region with the exception of the midline. In germband extended embryos, expression is seen in selected neurons and their support cells. Expression is also seen in some lateral and dorsal clusters of PNS cells, in the ligament cells of the chordotonal organs, and in two types of neurons (bipolar dendrite neurons and dendritic arbor cells). (Lloyd and Sakonju, 1991) In cellular blastoderm stage embryos, pdm2 is expressed in a broad band from 20-40% egg length and at the dorsal tip of the embryo. This pattern is replace by a complicated pattern of stripes. After gastrulation, 14 stripes are observed, one per parasegment. In later embryos, expression is seen in a resticted set of cells in the CNS. Maximum levels of pdm2 RNA are observed between 4 and 12 hours of embryonic development on northern blots. (Prakash et al., 1992) pdm2 transcripts are detected predominantly in 3-6 hr embryo RNA. They are expressed in the blastoderm embryo in a band of staining from 25-50% egg length and in an anterior cap. During gastrulation, a striped pattern is observed. Later pdm2 expression is observed in discrete subsets of neuroblasts in the developing CNS, in the brain, and in the hindgut. (Billin et al., 1991)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11   ganglion mother cell GMC4-2a   (Yeo et al., 1995) neuroblast NB3-3   (Tsuji et al., 2008)
Additional Descriptive Data
	pdm2 is expressed in early stages of the developing CNS in a highly dynamic pattern that includes roughly half of the neuroblasts and several ganglion mother cells. Here, nub and pdm2 are shown to be coexpressed in GMC4-2a but not in its progenitor neuroblast or its mature progeny. (Yeo et al., 1995)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from mutant phenotype (assigned by UniProtKB) (Prakash et al., 1992)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    17.8 Larval Midgut    64.4 Larval Hindgut    34.2 Larval Malpighian Tubules    6.1 Larval Fat Body    4.2 Larval Salivary Gland    5.2 Larval Trachea    1.975 Larval Carcass    8.775 Adult Head    10.7 Adult Eye    2.925 Adult Brain    32.7 Adult Thoracic-Abdominal Ganglion    63.8 Adult Crop    12.3 Adult Midgut    75.3 Adult Hindgut    35.9 Adult Malpighian Tubules    6.5 Adult Fat Body    7.2 Adult Salivary Gland    10.3 Adult Heart    1.825 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    2.8 Adult Testis    4.5 Adult Male Accessory Gland    10.6 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    10.7   NA  Eye    2.925   NA  Brain    32.7   17.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    63.8   NA  Crop    12.3   64.4  Midgut    75.3   34.2  Hindgut    35.9   6.1  Malpighian Tubules    6.5   4.2  Fat Body    7.2   5.2  Salivary Gland    no informative data   NA  Heart    1.825   1.975  Trachea    NA   NA  VirginFemale Spermatheca    5.5   NA  InseminatedFemale Spermatheca    7.2   NA  Ovary    2.8   NA  Testis    4.5   NA  Male Accessory Gland    no informative data   8.775  Carcass    6.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr    52 embryo 06-08hr    27 embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr  (52) embryo 06-08hr  (27) embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr    52 embryo 06-08hr    27 embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr    52 embryo 06-08hr    27 embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr    52 embryo 06-08hr    27 embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr  (52) embryo 06-08hr  27 embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr    52 embryo 06-08hr    27 embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    13 embryo 04-06hr    52 embryo 06-08hr    27 embryo 08-10hr    15 embryo 10-12hr    10 embryo 12-14hr    7 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    4 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    1 adult male 30day    1 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_31 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • CG12287 RE34565 RE49429 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004394 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12287-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for pdm2 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal pdm2Δmiti.Hsp83 lethal | heat sensitive pdm2Δmiti.hs viable pdm2d09994 viable, with Scer\GAL4pnr-MD237 pdm2GD5046 Sterility Allele fertile pdm2d09994 Other Phenotypes Allele neuroanatomy defective | embryonic stage pdm2Scer\UAS.T:Ivir\HA1 neuroanatomy defective | embryonic stage, with Scer\GAL4pros.PMG pdm2Scer\UAS.T:Ivir\HA1 Phenotype manifest in Allele embryonic abdominal segment 2 pdm2Δmiti.Hsp83 embryonic abdominal segment 2 | heat sensitive pdm2Δmiti.hs embryonic abdominal segment 6 | heat sensitive pdm2Δmiti.hs embryonic ganglion mother cell | ectopic pdm2hs.P ganglion mother cell pdm2Δmiti.Hsp83 ganglion mother cell | heat sensitive pdm2Δmiti.hs ganglion mother cell GMC4-2a pdm2E46 larval abdominal segment 1 pdm2hs.PC larval abdominal segment 2 pdm2hs.PC larval abdominal segment 3 pdm2hs.PC larval head pdm2hs.PC neuroblast NB3-3 | embryonic stage pdm2Scer\UAS.T:Ivir\HA1 neuroblast NB4-2 pdm2hs.P RP2 motor neuron pdm2Δmiti.Hsp83 pdm2E45 pdm2E46 pdm2PR13 pdm2hs.P RP2 motor neuron | ectopic pdm2hs.P RP2 motor neuron | heat sensitive pdm2Δmiti.hs RP2sib neuron pdm2Δmiti.Hsp83 RP2sib neuron | heat sensitive pdm2Δmiti.hs U3 neuron, with Scer\GAL4en-e16E pdm2Scer\UAS.T:Ivir\HA1 U4 neuron, with Scer\GAL4en-e16E pdm2Scer\UAS.T:Ivir\HA1 U5 neuron | supernumerary, with Scer\GAL4en-e16E pdm2Scer\UAS.T:Ivir\HA1 U5 neuron | supernumerary, with Scer\GAL4pros.PMG pdm2Scer\UAS.T:Ivir\HA1
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of pdm2 Class Mutagen Stocks Known lesion pdm2d09994 P-element activity 2 -- pdm2E46 antimorphic allele - genetic evidence ethyl methanesulfonate 1 Yes pdm2f02059 1 -- pdm2f05144 piggyBac transposase 1 -- pdm2MB00894 1 -- pdm2MI02425 1 -- pdm2MI03610 1 -- pdm2MI05029 1 -- pdm21602 Delta2-3 0 Yes pdm2971 Delta2-3 0 Yes pdm2E45 ethyl methanesulfonate 0 Yes pdm2PR13 Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of pdm2 Class Mutagen Stocks Known lesion pdm2GD16857 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pdm2GD5046 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pdm2JF02405 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pdm2KK110854 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pdm2hs.P in vitro construct - regulatory fusion 0 Yes pdm2hs.PC in vitro construct - regulatory fusion 0 Yes pdm2Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes pdm2Δmiti.hs in vitro construct - regulatory fusion 0 Yes pdm2Δmiti.Hsp83 antimorphic allele - genetic evidence in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6034 (Ryder, 2004.4.26) Df(2L)GR4 (Brewster et al., 2001, Yeo et al., 1995) Df(2L)pdm2GR19 (Yeo et al., 1995) Df(2L)pdm-2PR6 (Yeo et al., 1995) Df(2L)prd1.7 (Bhat and Schedl, 1994, Yeo et al., 1995)
Duplicated in	Dp(2;2)GYL (Bhat and Schedl, 1994)
Not disrupted in	Df(2L)Prl (Bhat and Schedl, 1994, Yeo et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hsp70-pdm2} NA P{hsp70-Δmiti} NA P{hsp83-Δmiti} NA P{hs-pdm-2} NA reporter construct P{pdm2-lacZ.+1} No P{pdm2-lacZ.+3} No UAS construct P{GD5046} NA P{GD16857} NA P{KK110854} NA P{TRiP.JF02405} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}pdm2MI02425 NA Mi{MIC}pdm2MI03610 NA Mi{MIC}pdm2MI05029 NA P{?white-un4}pdm2PR13 NA P{white-un4}pdm2971 NA P{white-un4}pdm21602 NA PBac{WH}pdm2f02059 NA PBac{WH}pdm2f05144 NA insertion of mobile activating element P{XP}pdm2d09994 NA insertion of enhancer trap binary system Mi{ET1}pdm2MB00894 No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from mutant phenotype (assigned by UniProtKB) (Prakash et al., 1992) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription inferred from direct assay (Junell et al., 2007)
Biological Process
CV term Evidence References
central nervous system development inferred from mutant phenotype (assigned by UniProtKB) (Prakash et al., 1992) ectoderm development inferred from mutant phenotype (assigned by UniProtKB) (Prakash et al., 1992) neuroblast development inferred from mutant phenotype (Tsuji et al., 2008) regulation of transcription, DNA-dependent inferred from mutant phenotype (assigned by UniProtKB) (Prakash et al., 1992)
Cellular Component
CV term Evidence References
nucleus inferred from mutant phenotype (assigned by UniProtKB) (Prakash et al., 1992)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 1993.7.1) sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR001356, InterPro:IPR017970 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with UniProtKB:P09086 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1993.7.1)
Biological Process
CV term Evidence References
nervous system development non-traceable author statement (Swiss-Prot Project Members, 1993.7.1) regulation of transcription, DNA-dependent inferred from sequence or structural similarity with UniProtKB:P09086 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with UniProtKB:P09086 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1993.7.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions pdm2 allele Gene References pdm2hs.P numb (Bhat and Apsel, 2004) unspecified D (Nambu et al., 1997)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60717 DroID - A comprehensive database of gene and protein interactions. • FBgn0004394 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34673
Orthologs
OrthoDB Orthologs (75) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG67M2HG Dipteran inclusive ortholog search EOG6C882J Insect inclusive ortholog search EOG6J6QN7 Arthropod inclusive ortholog search EOG6SJ3X3 Metazoa inclusive ortholog search EOG6KT2CG
Orthologs in Drosophila Species (EOG67M2HG)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\pdm2     Drosophila simulans   Dsim\GD23847 Y   Drosophila sechellia   Dsec\GM26881 Y   Drosophila erecta   Dere\GG23792 Y   Drosophila yakuba   Dyak\GE18596 Y   Drosophila ananassae   Dana\GF14202 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11534 Y   Drosophila persimilis   Dper\GL19359 Y   Drosophila willistoni   Dwil\GK15507 Y   Drosophila virilis   Dvir\pdm2 Y   Drosophila mojavensis   Dmoj\GI14490 Y   Drosophila grimshawi   Dgri\GH10117 Y
Orthologs in non-Drosophila Dipterans (EOG6C882J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL017445 Y Anopheles gambiae Malaria mosquito  Agam\AGAP009500 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801882 Y
Orthologs in non-Dipteran Insects (EOG6J6QN7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB53100 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003156 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16664 Y Atta cephalotes Leafcutter ant  Acep\ACEP11222 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO20413 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23101 Y Linepithema humile Argentine ant  Lhum\LH15637 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB19068 Y Solenopsis invicta Red fire ant  Sinv\SINV24231 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI005847 Y Pediculus humanus Human body louse  Phum\PHUM348630 Y Tribolium castaneum Red flour beetle  Tcas\TC007645 Y
Orthologs in non-Insect Arthropods (EOG6SJ3X3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_7063 Y
Orthologs in non-Arthropod Metazoa (EOG6KT2CG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ceh-6 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016443gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009262gn Y Danio rerio Zebrafish  Drer\zgc:136377 Y Danio rerio Zebrafish  Drer\OTTDARG00000033179 Y Danio rerio Zebrafish  Drer\pou1f1 Y Danio rerio Zebrafish  Drer\pou2f1b Y Danio rerio Zebrafish  Drer\pou3f3b Y Danio rerio Zebrafish  Drer\brn1.2 Y Danio rerio Zebrafish  Drer\pou3f2 Y Danio rerio Zebrafish  Drer\si:dkey-3j24.1 Y Danio rerio Zebrafish  Drer\pou3f3a Y Danio rerio Zebrafish  Drer\pou3f1 Y Xenopus tropicalis Western clawed frog  Xtro\oct91 Y Xenopus tropicalis Western clawed frog  Xtro\pou1f1 Y Xenopus tropicalis Western clawed frog  Xtro\pou3f4 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000006231 Y Xenopus tropicalis Western clawed frog  Xtro\pou3f1 Y Xenopus tropicalis Western clawed frog  Xtro\pou3f2 Y Xenopus tropicalis Western clawed frog  Xtro\pou3f3 Y Xenopus tropicalis Western clawed frog  Xtro\pou2f3 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033156 Y Gallus gallus Domestic chicken  Ggal\POU2F3 Y Gallus gallus Domestic chicken  Ggal\POU1F1 Y Mus musculus House mouse  Mmus\Pou3f2 Y Mus musculus House mouse  Mmus\Pou3f1 Y Mus musculus House mouse  Mmus\Pou5f1 Y Mus musculus House mouse  Mmus\ENSMUSG00000008496 Y Mus musculus House mouse  Mmus\Pou2f3 Y Mus musculus House mouse  Mmus\Pou1f1 Y Mus musculus House mouse  Mmus\Pou3f3 Y Mus musculus House mouse  Mmus\Pou3f4 Y Rattus norvegicus Norway rat  Rnor\Pou2f2 Y Rattus norvegicus Norway rat  Rnor\Pou3f4 Y Rattus norvegicus Norway rat  Rnor\Pou3f3 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000000715 Y Rattus norvegicus Norway rat  Rnor\Pou3f2 Y Rattus norvegicus Norway rat  Rnor\Pou2f3 Y Homo sapiens Human  Hsap\POU3F3 Y Homo sapiens Human  Hsap\POU3F1 Y Homo sapiens Human  Hsap\POU3F4 Y Homo sapiens Human  Hsap\POU3F2 Y Homo sapiens Human  Hsap\POU2F2 Y Homo sapiens Human  Hsap\POU1F1 Y Homo sapiens Human  Hsap\POU2F3 Y Homo sapiens Human  Hsap\POU5F1P1 Y Homo sapiens Human  Hsap\POU5F1 Y
Human Orthologs (9)
Gene OMIM HGNC Hsap\POU3F3     Hsap\POU3F1     Hsap\POU3F4     Hsap\POU3F2     Hsap\POU2F2     Hsap\POU1F1     Hsap\POU2F3     Hsap\POU5F1P1     Hsap\POU5F1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23847 Drosophila sechellia Dsec\GM26881 Drosophila erecta Dere\GG23792 Drosophila yakuba Dyak\GE18596 Drosophila ananassae Dana\GF14202 Drosophila pseudoobscura pseudoobscura Dpse\GA11534 Drosophila persimilis Dper\GL19359 Drosophila willistoni Dwil\GK15507 Drosophila virilis Dvir\pdm2 Drosophila mojavensis Dmoj\GI14490 Drosophila grimshawi Dgri\GH10117
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	8855 pdm2E46/CyO 19325 w1118; P{XP}pdm2d09994 29543 y1 v1; P{TRiP.JF02405}attP2 35088 y1 w*; Mi{MIC}pdm2MI02425 37064 y1 w*; Mi{MIC}pdm2MI03610 38085 y1 w*; Mi{MIC}pdm2MI05029
Harvard	- P{XP}pdm2d09994 - PBac{WH}pdm2f02059 - PBac{WH}pdm2f05144
VDRC	v102126 P{KK110854}VIE-260B v30708 w1118; P{GD5046}v30708 v52272 w1118; P{GD16857}v52272/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR28A12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 17 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE34565 RE49429
Other clones	FI17318 MIP04269
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE51329
Other clones	97627 97906 124791 281228 CK01.121A.H8 CK00427 EC15179 EK092664 EK220352 EK306154 EP14112 RP001025900
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004394 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34673
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: pdm2 CG15486 CG15487 (FlyBase Genome Annotators, 2002-2003)
Additional comments	Annotations CG12287, CG15486 and CG15487 merged as CG12287 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	nub/pdm2 and cas regulate late-born motor neuron identity within the NB7-1 lineage; nub/pdm2 specifies fourth-born U4 motor neuron identity, while nub/pdm2/cas together specify fifth-born U5 motor neuron identity. (Grosskortenhaus et al., 2006) Virtually all aspects of pdm2 RNA expression are conserved between D.melanogaster and D.virilis. (Poole, 1995) nub and pdm2 both contribute toward the development of a full complement of mature RP2 neurons by being expressed in and acting on ganglion mother cell 2a (GMC-2a), the RP2 progenitor cell. nub plays a quantitatively less important role than pdm2. nub and pdm2 act to specify GMC-2a cell identity and not merely act to positively regulate GMC-2a cell division. Within the hierarchy of genes expressed in GMC4-2a nub and pdm2 lie downstream of pros and ftz and upstream of eve. (Yeo et al., 1995) pdm2 is required for segmentation and neurogenesis. (Bhat and Schedl, 1994) The genomic organisation and early embryonic expression pattern of pdm2 has been determined. (Cockerill et al., 1993) The pdm2 gene product specifies the cell fate of GMC-1 in the NB4-2 lineage. Its ectopic expression in the two progeny cells of GMC-1 is sufficient to cause both the cells to adopt a GMC-1 cell identity. (Yang et al., 1993) pdm2 is transcribed in elements of the developing nervous system suggesting a functional role in neurogenesis and neurospecification. (Dick et al., 1992) pdm2 has been cloned and its spatial expression patterns characterized. pdm2 belongs to the class II type of POU genes. The pdm2 gene product is an octamer-dependent transcription factor that is capable of activating transcription in yeast. (Prakash et al., 1992) pdm2 contains a POU domain, and is expressed at maximum levels during early development. (Billin et al., 1991) The cDNA sequences of pdm2 and nub and their developmental expression patterns have been determined. (Dick et al., 1991) The spatial expression pattern of pdm2 has been characterized. (Lloyd and Sakonju, 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078834 NM_165017 RefSeq (Proteins) • NP_523558 NP_723763 DDBJ / EMBL / GenBank • AAA28481 AAA28732 AAA28830 AAB21408 AAF53208 AAF53209 AAQ23557 AAQ23575 BI238172 BI364340 BT010239 BT010257 CZ487765 M65016 M81958 M93149 S80559 Entrez Gene - A searchable database of RefSeq genes. • 34673 UniProtKB/Swiss-Prot • P31369 Q9VK71
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Homeobox, conserved site (IPR017970) POU-specific (IPR000327) Homeodomain (IPR001356) Homeodomain-like (IPR009057) Lambda repressor-like, DNA-binding domain (IPR010982) POU domain (IPR013847) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • CG12287 RE34565 RE49429
Linkouts
	BioGRID - A database of protein and genetic interactions • 60717 DroID - A comprehensive database of gene and protein interactions. • FBgn0004394 DRSC - Results from RNAi screens. • FBgn0004394 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004394 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12287-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004394 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34673 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/pdm-2.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34673 modMine - Data generated by the modENCODE project. • FBgn0004394 REDfly - A database of transcriptional regulatory elements. • FBgn0004394
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	CG12287 (Klebes et al., 2005, Kreiman, 2004, Keleman et al., 2009.8.5, Perkins et al., 2009, Macdonald and Long, 2006) CG15486 (Manning et al., 2012) CG15487   dim (Prakash et al., 1992, ) dOct2 (Prakash et al., 1992, ) dPOU28 (Yeo et al., 1995, Yang et al., 1993, Dick et al., 1992, ) dpou28 (Jan and Jan, 1994) dPOU-28 (Goodman and Doe, 1993, Cabrera, 1992, Dick et al., 1991, ) dPOU28/pdm-2 (Yang et al., 1993, Jimenez and Modolell, 1993, Yang et al., 1993) miti (Bhat and Apsel, 2004, Mehta and Bhat, 2001, Bhat et al., 1995, Bhat and Schedl, 1993, Bhat and Schedl, 1994) Oct-2 (Malin et al., 2005) Oct2 (Malin et al., 2005) pdm (Rougvie, 2005, Grosskortenhaus et al., 2005, Abbott, 2003, Harris, 2001, Pearson and Doe, 2003, Kanai et al., 2003, Bellaiche and Schweisguth, 2001, Odenwald, 2005, Tran and Doe, 2007, Tsuji et al., 2008, Nakajima et al., 2010) Pdm (Grosskortenhaus et al., 2006) pdm-2 (Brody et al., 2005, Brody et al., 2003, Edenfeld et al., 2002, Brody and Odenwald, 2002, Bergman and Kreitman, 2001, Hemavathy et al., 2000, Ashraf et al., 2000, Lloyd, 1995.12.4, Billin, 1991.12.16, Ashraf et al., 1999, Eisen, 1998, Nambu et al., 1997, Ryan and Rosenfeld, 1997, Odenwald et al., 1997, Odenwald et al., 1996, McDonald and Doe, 1996, Skeath, 1995, McDonald et al., 1995, Poole, 1995, Jan and Jan, 1994, Manning and Krasnow, 1993, Cockerill et al., 1993, Billin et al., 1991, Ishihara and Shibata, 2008) Pdm2 (Fichelson et al., 2005, Rebeiz et al., 2011, Tsuji et al., 2008, Mace et al., 2010) Pdm-2 (Ma et al., 2000, Odenwald et al., 1999, Brody and Odenwald, 1999, Brody and Odnewald, 1999, Brody and Odenwald, 1999, Nakagoshi et al., 1998) pdm2 (Grosskortenhaus et al., 2006, Wheeler et al., 2006, Jacobsen et al., 2006, Kreiman, 2004, Junell et al., 2007, Segal et al., 2008, Hooper et al., 2007, Berman et al., 2004, Turchyn et al., 2011, Wheeler et al., 2008, Tran and Doe, 2008, Yu and Small, 2008, Gaziova and Bhat, 2009, Stagg et al., 2011, Tomancak et al., 2007, Macdonald and Long, 2006, Tran et al., 2010, Kazemian et al., 2010, Kao et al., 2012, Nikulova et al., 2012, Manning et al., 2012) POU domain protein 2 (Edwards et al., 2006)
Name Synonym	didymous   miti-mere (Odenwald et al., 1996, Bhat and Schedl, 1994) Miti-mere (Bhat and Schedl, 1993) POU domain prot 2 (Carpenter et al., 2009) POU domain protein 2 (Edwards et al., 2006, Wheeler et al., 2006, Berman et al., 2004, Yu and Small, 2008, Macdonald and Long, 2006)
Secondary FlyBase IDs
	FBgn0032441 FBgn0032443
References ( 131 )
Generate a list of	All references relating to this gene ( 131 )
List References by type	Research paper  ( 60 ) Supplementary material  ( 3 ) Review  ( 20 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 23 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 7 )
	Kao et al., 2012, Neuron 73(4): 677--684 Hierarchical deployment of factors regulating temporal fate in a diverse neuronal lineage of the Drosophila central brain. [FBrf0217643] Manning et al., 2012, Cell Rep. 2(4): 1002--1013 A Resource for Manipulating Gene Expression and Analyzing cis-Regulatory Modules in the Drosophila CNS. [FBrf0219785] Nikulova et al., 2012, Nucleic Acids Res. 40(12): e93 CORECLUST: identification of the conserved CRM grammar together with prediction of gene regulation. [FBrf0218757] Gabilondo et al., 2011, Mech. Dev. 128(3-4): 208--221 A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Rebeiz et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(25): 10036--10043 Evolutionary origin of a novel gene expression pattern through co-option of the latent activities of existing regulatory sequences. [FBrf0214016] Stagg et al., 2011, Development 138(11): 2171--2183 Dual role for Drosophila lethal of scute in CNS midline precursor formation and dopaminergic neuron and motoneuron cell fate. [FBrf0213671] Turchyn et al., 2011, Dev. Biol. 357(1): 83--95 Evolution of nubbin function in hemimetabolous and holometabolous insect appendages. [FBrf0214671] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...