dCREB-A, CREB, BBF-2, dCREBA, Creb-A
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.50
May bind DNA as heterodimers with other bZIP proteins.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CrebA using the Feature Mapper tool.
Comment: reported as salivary gland body specific anlage
CrebA transcripts are observed throughout development on northern blots with the highest levels in embryos and adults males. RNAse protection experiments show equivalent levels of CrebA transcripts in third instar larval gut, fat body, and trachea.
In embryos, CrebA transcripts are expressed in the developing salivary gland. In the adult, transcripts are found in the brain and optic lobe cell bodies, in salivary gland, and in midgut epithelial cells of the cardia. In females CrebA transcripts are expressed in columnar follicle cells. In males, they are expressed in the seminal vesicle, ejaculatory duct, and ejaculatory bulb.
GBrowse - Visual display of RNA-Seq signalsView Dmel\CrebA in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
In the epidermis CrebA is required for patterning cuticular structures on both the dorsal and ventral surfaces of larvae, mutant larvae have only lateral structures around the entire circumference of each segment. Epistasis tests with known dorsal/ventral patterning genes suggests that CrebA encodes a transcription factor near the end of both the dpp and spi signalling cascades to translate the corresponding extracellular signals into changes in gene expression. Double mutants with segment polarity genes confirm that CrebA is not involved in segment polarity.
The genomic organisation of the CrebA locus has been characterised, and its expression pattern has been analysed.
CrebA mutants display lateralisation of the larval cuticle and the cuticle is significantly weaker than wild type larval cuticle. The salivary gland and tracheae display a 'crooked' phenotype.
Isolation and characterization of Creb reveals that the protein binds to the CRE sequence and belongs to the leucine zipper family of transcription factors.