tamas, tam, DNApol-γ125, l(2)34Dc, pol γ-α
Gene model reviewed during 5.41
Gene model reviewed during 5.45
There is only one protein coding transcript and one polypeptide associated with this gene
Component of the DNA polymerase gamma complex consisting of two subunits: the catalytic subunit DNApol-gamma/DNApolG1 and the accessory subunit DNApol-gamma35/DNApolG2.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PolG1 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\PolG1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Multiple amino acid sequence alignments show the DNA polymerases from numerous species forms a family strongly conserved from yeast to man.
Cloning and biochemical characterisation of tam demonstrates both 5' to 3' DNA polymerase activity and 3' to 5' exonuclease activity in the recombinant protein. tam polypeptide amino acid sequence is 1145 residues, with a predicted molecular mass of 129.9kD.
Subunit structure and enzymatic activity of DNA polymerase γ are studied using a combination of physical and immunological approaches. The role of the 2 subunits in template primer DNA binding is explored.
The rate and processivity of DNA synthesis by DNA polymerase γ has been analysed under a variety of different conditions.
Biochemical description of the 3'->5' exonuclease associated with mitochondrial DNA polymerase.
The 3' to 5' exonuclease encoded provides a proof-reading function to enhance the fidelity of DNA synthesis during mitochondrial DNA replication.
A structural and mechanistic analysis of DNApol-γ125 and DNApol-γ35 demonstrates them to be both effective and accurate in the synthesis of DNA.
Comparison of the rate and specificity of DNA synthesis by DNApol-γ125 and DNApol-γ35 on several template primers, and an examination of the products of DNA synthesis has been carried out.
DNApol-γ125 and DNApol-γ35 have been isolated and purified and their subunit structure and catalytic properties examined.
The gene is named "tamas" (Sanskrit meaning "dark inertia") based on the mutant phenotype of a reduction in the larval response to light.