• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\DNApol-γ35

General Information
Symbol	Dmel\DNApol-γ35	Species	D. melanogaster
Name	DNA polymerase γ 35kD	Annotation symbol	CG33650
Feature type	protein_coding_gene	FlyBase ID	FBgn0004407
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	l(2)34De, pol γ-β, MtPolB, l(2)br16, polγ, DNApol-γ, BG:DS00941.9, br16
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	34D1-34D1	Sequence location	2L:13,831,482..13,833,167 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene DNA polymerase γ 35kD is referred to in FlyBase by the symbol Dmel\DNApol-γ35 (CG33650, FBgn0004407). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA-directed DNA polymerase activity. There is experimental evidence that it is involved in the biological process: DNA-dependent DNA replication; mitochondrial genome maintenance; mitochondrion organization. 5 alleles are reported. The phenotypes of these alleles are annotated with: mitochondrion; embryonic/larval central nervous system. It has one annotated transcript and one annotated polypeptide. Protein features are: Anticodon-binding; Glycyl-tRNA synthetase, alpha2 dimer. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1637875_a_at aligns to a genomic region shared by exons of 2 FlyBase-annotated genes; hence it is impossible to interpret the expression pattern and levels of DNApol-gamma35 expression signal. See the GBrowse view of DNApol-gamma35 to determine the overlapping gene(s). Gene sequence location is 2L:13831482..13833167.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 34D1-34D1   Limits computationally determined from genome sequence between P{lacW}Sosk05224&P{lacW}RpII33k05605 and P{lacW}l(2)34Fak00811&P{PZ}wb09437
Experimentally Determined Cytological Location
Cytogenetic map Notes References 34D-34E     (Kaguni, 1997.3.20)
Experimentally Determined Recombination Data
Location
Left of (cM)	RpII33 (Ashburner et al., 1999)
Right of (cM)
Notes	Maps between Sop2 or Orc5 and RpII33. (Ashburner et al., 1999)
Gene Model & Products
Please see the GBrowse view of Dmel\DNApol-γ35 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_dicistronic_mRNA ; SO:0000722 Gene model reviewed during 5.41
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) DNApol-γ35-RA FBtr0091627  NM_001032091   1632   361
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein DNApol-γ35-PA   FBpp0099758   41.0   361   7.41     NP_001027262   AAF53341
Additional Polypeptide Data & Comments
Reported size (kDa)	35 (kD) (Wernette and Kaguni, 1986, Wernette et al., 1988, Kaguni et al., 1988)
Comments	Mitochondrial DNA polymerase was purified to near homogeneity from early embryos. It is a heterodimer consisting of two subunits of 125kD and 35kD. The large subunit contains the DNA-polymerase activity.The DNA polymerase utilizes a large variety of template-primers efficiently. (Wernette and Kaguni, 1986)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Anticodon-binding (IPR004154) Glycyl-tRNA synthetase, alpha2 dimer (IPR002315) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME08969
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695573   AE014134 AAF53341   AE003408 AAF44825   AI106934   AI238599   AI297248   AI513318   AI514928   AY051491 AAK92915   BF488488   BF489699   BF489738   BF490117   BF490171   BF490439   BF498448   BF500952   BI354456   BI354458   BI357166   BI361464   BI363980   BI365536   BI481621   BI482461   BI482883   BI570289   BI604720   BI613659   BI635859   BI641805   CK658179   CL527895   CO268805   CO333367   DN154135   EC063156   EC205381   GH747984   GH748490   GH748603   GH748718   GH749620   GH750381   GH750489   GH750821   GH750836   GH751027   GH751664   GH751987   GH752309   GH752383   GH753128   GH753680   GH753838   GH754173   GH754614   GH755136   GH757544   GH757989   GH758336   GH760495   GH760705   GH760755   GH761065   GH770274   GH770762   GH770804   GH771157   GH772150   GH772358   GH772633   GH772735   GH772873   GH773037   GH773294   GH773614   GH773907   GH773929   GH773992   GH774101   GH774926   GH774960   GH775202   GH775908   GH776051   GH776287   GH776334   GH777804   GH777992   GH778366   GH778525   GH778985   GH780620   GH780745   GH782107   GH782729   GH783136   GH784072   GH784170   GH784596   GH784605   GH784620   GH784751   GH784906   GH784914   GH785076   GH785552   GH785908   GH786695   GH787074   GH787107   GH787322   GH787873   GH787966   GH788502   GH788703   GH788769   GH789167   GH790074   GH790565   GH790751   GH791033   GH791353   GH791451   GH791744   GH793312   GH793433   GH793590   GH793974   GH794017   GH794193   GH795040   GH795100   GH795339   GH795502   GH796629   GH796786   GH797273   GH797445   GH798334   GH798933   GH799364   GH800535   GH801428   GH801537   GH802104   GH802558   GH803110   GH803745   GH804935   GH805230   GH805481   GH805712   GH806460   GH806713   GH807223   GH807460   GH808318   GH808647   GH809351   GH809697   GH809753   GH809819   GH809919   GH809947   GH809950   GH810017   GH810605   GH810770   GH811439   GH811452   GH811570   GH812246   GH812604   GH813205   GH813443   GH813912   GH813983   GH815228   GH815423   GH815674   GH815855   GH816249   GH816404   GH817018   GH817047   GH817054   GH817590   GH817716   GH818289   GH821545   GH821588   GH821733   GH823858   GH824738   GH825188   GH826270   GH827607   GH828930   GH829513   GH829772   GH830447   GH831733   GH832005   GH832084   GH832553   GH832638   GH832957   GH833000   GH833060   GH833612   GH833804   GH833895   GH835315   GH836403   GH836460   GH836727   GH836966   GH837078   GH837758   GH837760   GH838672   GH838791   GH838913   GH838957   GH839582   GH839597   GH840013   GH842785   GH843144   GH843897   GH843917   GH844175   GH844505   GH844711   GH846003   GH846645   GH846884   GH846930   GH848047   GH849065   GH849658   GH850912   GH852058   GH852114   GH852214   GH852616   GH852751   GH854423   GH854444   GH854635   GH855009   GH855185   GH856231   GH857681   GH857757   GH859980   GH860331   GH860862   GH861040   GH861046   GH861050   GH861475   GH862512   GH862615   GH862643   GH863124   GH863660   GH864203   GH865223   GH865608   GH865867   GH866069   GH866195   GH866486   GH866512   GH867145   GH868125   GH869227   GH869575   GH869643   GH871144   GH871204   GH871418   GH873021   GH873446   GH876977   GH876986   GH877062   GH877393   GH877657   GH879185   GH879253   GH879448   GH880209   GH880885   GH882662   GH882816   GH885976   GH886459   GH886709   GH886845   GH887127   GH887624   GH887933   GH888955   GH889041   GH889499   GH889739   GH890417   GH890468   GH891436   GH892446   GH893738   GH894256   GH894293   GH894503   GH894780   GH895842   GH895857   GH896935   GH898163   GH898503   GH898618   GH898984   GH899217   GH899817   GH900338   GH900921   GH900984   GH901387   GH902112   GH902350   GH902565   GH903121   GH904090   GH904426   GH905033   GH905197   GH905353   GH906042   GH906499   GH906658   GH906820   GH907107   GH907550   GH907705   GH907992   GH908773   GH908954   GH908987   GH909293   GH909647   GH909810   GH909966   GH910732   GH910868   GH911493   GH911501   GH911969   GH912550   GH912652   GH914235   GH915480   GH915932   GH916886   GH919314   GH919867   GH920076   GH920348   GH920481   GH921986   GH922240   GH922977   GH923560   GH925455   GH925502   GH926115   GH926228   GH926586   GH926730   GH926748   GH927438   GH927546   GH927673   GH927911   GH929375   GH929462   GH929958   GH930417   GH930786   GH931084   GH932023   GH932173   GH932203   GH932724   GH933669   GH934657   GH934853   GH934932   GH935257   GH935330   GH935413   GH935578   GH936394   GH936587   GH936635   GH936881   GH937286   GH937367   GH937549   GH937852   GH937933   GH938147   GH938725   L39629   U94702 AAC47536
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O02005 Q9VJV8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0004407    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0004407 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0004407 expression level Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr    1667 embryo 08-10hr    1743 embryo 10-12hr    2263 embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1    1779 larva L2    1850 larva L3 12hr old    1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9    1508 white prepupae new    1366 white prepupae 12hr    1658 white prepupae 24hr    2085 pupae 2d postWPP    2323 pupae 3d postWPP    2305 pupae 4d postWPP    1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day    1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (421) embryo 02-04hr  (681) embryo 04-06hr  (1304) embryo 06-08hr  (1667) embryo 08-10hr  (1743) embryo 10-12hr  (2263) embryo 12-14hr  (1349) embryo 14-16hr  (1267) embryo 16-18hr  (987) embryo 18-20hr  (764) embryo 20-22hr  (919) embryo 22-24hr  (1023) larva L1  (1779) larva L2  (1850) larva L3 12hr old  (1410) larva L3 puffstage 1-2  (1119) larva L3 puffstage 3-6  (1203) larva L3 puffstage 7-9  (1508) white prepupae new  (1366) white prepupae 12hr  (1658) white prepupae 24hr  (2085) pupae 2d postWPP  (2323) pupae 3d postWPP  (2305) pupae 4d postWPP  (1452) adult male 01day  (1288) adult male 05day  (1164) adult male 30day  (1296) adult female 01day  (1423) adult female 05day  (1176) adult female 30day  (1114) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr  (1667) embryo 08-10hr  (1743) embryo 10-12hr  (2263) embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1  (1779) larva L2  (1850) larva L3 12hr old  1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9  1508 white prepupae new    1366 white prepupae 12hr  (1658) white prepupae 24hr  (2085) pupae 2d postWPP  (2323) pupae 3d postWPP  (2305) pupae 4d postWPP  1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day  1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr    1667 embryo 08-10hr    1743 embryo 10-12hr    2263 embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1    1779 larva L2    1850 larva L3 12hr old    1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9    1508 white prepupae new    1366 white prepupae 12hr    1658 white prepupae 24hr    2085 pupae 2d postWPP    2323 pupae 3d postWPP    2305 pupae 4d postWPP    1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day    1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr    1667 embryo 08-10hr    1743 embryo 10-12hr    2263 embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1    1779 larva L2    1850 larva L3 12hr old    1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9    1508 white prepupae new    1366 white prepupae 12hr    1658 white prepupae 24hr    2085 pupae 2d postWPP    2323 pupae 3d postWPP    2305 pupae 4d postWPP    1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day    1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0004407 expression level Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr    1667 embryo 08-10hr    1743 embryo 10-12hr    2263 embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1    1779 larva L2    1850 larva L3 12hr old    1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9    1508 white prepupae new    1366 white prepupae 12hr    1658 white prepupae 24hr    2085 pupae 2d postWPP    2323 pupae 3d postWPP    2305 pupae 4d postWPP    1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day    1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (421) embryo 02-04hr  (681) embryo 04-06hr  (1304) embryo 06-08hr  (1667) embryo 08-10hr  (1743) embryo 10-12hr  (2263) embryo 12-14hr  (1349) embryo 14-16hr  (1267) embryo 16-18hr  (987) embryo 18-20hr  (764) embryo 20-22hr  (919) embryo 22-24hr  (1023) larva L1  (1779) larva L2  (1850) larva L3 12hr old  (1410) larva L3 puffstage 1-2  (1119) larva L3 puffstage 3-6  (1203) larva L3 puffstage 7-9  (1508) white prepupae new  (1366) white prepupae 12hr  (1658) white prepupae 24hr  (2085) pupae 2d postWPP  (2323) pupae 3d postWPP  (2305) pupae 4d postWPP  (1452) adult male 01day  (1288) adult male 05day  (1164) adult male 30day  (1296) adult female 01day  (1423) adult female 05day  (1176) adult female 30day  (1114) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr  1667 embryo 08-10hr  1743 embryo 10-12hr  2263 embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1  1779 larva L2  1850 larva L3 12hr old  1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9  1508 white prepupae new    1366 white prepupae 12hr  1658 white prepupae 24hr  2085 pupae 2d postWPP  2323 pupae 3d postWPP  2305 pupae 4d postWPP  1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day  1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr    1667 embryo 08-10hr    1743 embryo 10-12hr    2263 embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1    1779 larva L2    1850 larva L3 12hr old    1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9    1508 white prepupae new    1366 white prepupae 12hr    1658 white prepupae 24hr    2085 pupae 2d postWPP    2323 pupae 3d postWPP    2305 pupae 4d postWPP    1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day    1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    421 embryo 02-04hr    681 embryo 04-06hr    1304 embryo 06-08hr    1667 embryo 08-10hr    1743 embryo 10-12hr    2263 embryo 12-14hr    1349 embryo 14-16hr    1267 embryo 16-18hr    987 embryo 18-20hr    764 embryo 20-22hr    919 embryo 22-24hr    1023 larva L1    1779 larva L2    1850 larva L3 12hr old    1410 larva L3 puffstage 1-2    1119 larva L3 puffstage 3-6    1203 larva L3 puffstage 7-9    1508 white prepupae new    1366 white prepupae 12hr    1658 white prepupae 24hr    2085 pupae 2d postWPP    2323 pupae 3d postWPP    2305 pupae 4d postWPP    1452 adult male 01day    1288 adult male 05day    1164 adult male 30day    1296 adult female 01day    1423 adult female 05day    1176 adult female 30day    1114 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0004407    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0004407 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0004407 expression level Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0004407 expression level Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    201.9 Larval Midgut    75 Larval Hindgut    123.8 Larval Malpighian Tubules    81.4 Larval Fat Body    74.6 Larval Salivary Gland    127.6 Larval Trachea    123.65 Larval Carcass    94.275 Adult Head    113.7 Adult Eye    160.9 Adult Brain    178.6 Adult Thoracic-Abdominal Ganglion    224.4 Adult Crop    116.3 Adult Midgut    66.3 Adult Hindgut    120.3 Adult Malpighian Tubules    159 Adult Fat Body    68.8 Adult Salivary Gland    136.8 Adult Heart    109.325 Adult VirginFemale Spermatheca    111.2 Adult InseminatedFemale Spermatheca    134.1 Adult Ovary    82.7 Adult Testis    86.5 Adult Male Accessory Gland    110.1 Adult Carcass    71.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    113.7   NA  Eye    160.9   NA  Brain    178.6   201.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    224.4   NA  Crop    116.3   75  Midgut    66.3   123.8  Hindgut    120.3   81.4  Malpighian Tubules    159   74.6  Fat Body    68.8   127.6  Salivary Gland    136.8   NA  Heart    109.325   123.65  Trachea    NA   NA  VirginFemale Spermatheca    111.2   NA  InseminatedFemale Spermatheca    134.1   NA  Ovary    82.7   NA  Testis    86.5   NA  Male Accessory Gland    110.1   94.275  Carcass    71.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004407 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33084-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage DNApol-γ351 DNApol-γ352 lethal | pupal stage (with Df(2L)b80c1) DNApol-γ351 DNApol-γ352 lethal | pupal stage (with DNApol-γ351) DNApol-γ352 lethal | pupal stage (with DNApol-γ352) DNApol-γ351 lethal | recessive DNApol-γ351 DNApol-γ352 Other Phenotypes Allele locomotor behavior defective (with Df(2L)b80c1) DNApol-γ352 neuroanatomy defective (with Df(2L)b80c1) DNApol-γ351 DNApol-γ352 neuroanatomy defective (with DNApol-γ351) DNApol-γ352 neuroanatomy defective (with DNApol-γ352) DNApol-γ351 Phenotype manifest in Allele embryonic/larval central nervous system (with Df(2L)b80c1) DNApol-γ351 embryonic/larval central nervous system (with DNApol-γ351) DNApol-γ352 embryonic/larval central nervous system (with DNApol-γ352) DNApol-γ351 mitochondrion (with Df(2L)b80c1) DNApol-γ351 DNApol-γ352 mitochondrion (with DNApol-γ351) DNApol-γ352 mitochondrion (with DNApol-γ352) DNApol-γ351
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of DNApol-γ35 Class Mutagen Stocks Known lesion DNApol-γ352 spontaneous ethyl methanesulfonate 10 Yes DNApol-γ351 ethyl methanesulfonate 2 Yes DNApol-γ35EY06366 P-element activity 1 Yes DNApol-γ353 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of DNApol-γ35 Class Mutagen Stocks Known lesion DNApol-γ35hs.PI in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)64j (Iyengar et al., 1999, Woodruff and Ashburner, 1979) Df(2L)b75 (Woodruff and Ashburner, 1979, Gubb et al., 1990) Df(2L)b80c1 (Iyengar et al., 1999, Iyengar et al., 2002, Ashburner et al., 1982) Df(2L)b81l42 (Roote, 1997.6.3) Df(2L)b83l1 (Roote, 1997.6.3) Df(2L)b84a9 (Iyengar et al., 1999, Roote, 1997.6.3) Df(2L)b85b1 (Roote, 1997.6.3) Df(2L)b88g83 (Roote, 1997.6.3) Df(2L)fn30 (Ashburner et al., 1982) Df(2L)fn31 (Ashburner et al., 1982) Df(2L)GT5 (Gubb et al., 1990) Df(2L)k07245Lk05605R-mr5 (Roote, 2000.6.13) Df(2L)k07245Lk05605R-mr9 (Roote, 2000.6.13) Df(2L)TE35B-5 (Gubb et al., 1985) Df(2L)TE35B-203 (Roote et al., 1996) Df(2L)TE35BC-6 (Gubb et al., 1984) Df(2L)TE35BC-23 (Gubb et al., 1984) In(2L)b88e45 (Roote, 1997.6.3) T(2;3)b89e12 (Roote, 1998.5.15, Ashburner et al., 1999, Roote, 2000.6.5) Tp(2;3)dp (Roote, 1997.6.3)
Not disrupted in	Df(2L)A47 (Ashburner et al., 1982, Gubb et al., 1990, Roote, 1996.8, Roote, 1997.6.3) Df(2L)A48 (Ashburner et al., 1982, Gubb et al., 1990) Df(2L)A63 (Ashburner et al., 1982) Df(2L)A72 (Ashburner et al., 1982) Df(2L)A178 (Ashburner et al., 1982) Df(2L)A215 (Ashburner et al., 1982) Df(2L)A217 (Ashburner et al., 1982) Df(2L)A220 (Ashburner et al., 1982) Df(2L)A245 (Ashburner et al., 1982) Df(2L)A246 (Ashburner et al., 1982) Df(2L)A260 (Ashburner et al., 1982) Df(2L)A263 (Ashburner et al., 1982) Df(2L)A264 (Ashburner et al., 1982) Df(2L)A266 (Ashburner et al., 1982) Df(2L)A267 (Ashburner et al., 1982) Df(2L)A376 (Ashburner et al., 1982) Df(2L)A377 (Ashburner et al., 1982) Df(2L)A379 (Ashburner et al., 1982) Df(2L)A400 (Ashburner et al., 1982) Df(2L)A445 (Ashburner et al., 1982) Df(2L)Adh-nBR55 (Roote, 1997.6.3) Df(2L)el77 (Woodruff and Ashburner, 1979) Df(2L)el80i1 (Roote, 1997.6.3) Df(2L)el82f1 (Roote, 1997.6.3) Df(2L)fn1 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn2 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn3 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn5 (Ashburner et al., 1982) Df(2L)fn7 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn12 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn15 (Ashburner et al., 1982) Df(2L)fn26 (Ashburner et al., 1982) Df(2L)fn27 (Ashburner et al., 1982) Df(2L)fn36 (Ashburner et al., 1982) Df(2L)GT4 (Gubb et al., 1990) Df(2L)Scorv4 (Woodruff and Ashburner, 1979) Df(2L)TE35B-1 (Gubb et al., 1985) Df(2L)TE35B-2 (Gubb et al., 1985) Df(2L)TE35B-3 (Gubb et al., 1985) Df(2L)TE35B-4 (Gubb et al., 1985) Df(2L)TE35B-6 (Gubb et al., 1985) Df(2L)TE35B-7 (Gubb et al., 1985) Df(2L)TE35B-8 (Gubb et al., 1985) Df(2L)TE35B-9 (Gubb et al., 1985) Df(2L)TE35B-10 (Gubb et al., 1985) Df(2L)TE35B-11 (Gubb et al., 1985) Df(2L)TE35B-12 (Gubb et al., 1985) Df(2L)TE35B-15 (Gubb et al., 1985) Df(2L)TE35B-407 (Roote, 1997.6.3) Df(2L)TE35BC-1 (Gubb et al., 1984) Df(2L)TE35BC-3 (Gubb et al., 1984) Df(2L)TE35BC-4 (Gubb et al., 1984) Df(2L)TE35BC-7 (Gubb et al., 1984) Df(2L)TE35BC-8 (Gubb et al., 1984) Df(2L)TE35BC-24 (Gubb et al., 1984) Df(2L)TE35BC-28 (Gubb et al., 1984) Df(2L)TE35BC-29 (Gubb et al., 1984) Df(2L)TE35BC-31 (Gubb et al., 1984) Df(2L)TE35BC-34 (Gubb et al., 1984) Df(2L)TE35BC-35 (Gubb et al., 1984) Df(2L)W (Woodruff and Ashburner, 1979) In(2L)75c (Woodruff and Ashburner, 1979) In(2L)75cLC158.1R (Woodruff and Ashburner, 1979) In(2L)TE35B-13 (Gubb et al., 1985) T(2;3)Scorv7 (Woodruff and Ashburner, 1979) T(Y;2)A80DR15P (Woodruff and Ashburner, 1979) T(Y;2)J165DP58P (Woodruff and Ashburner, 1979) T(Y;2)R15DJ165P (Woodruff and Ashburner, 1979) Tp(2;2)A446 (Ashburner et al., 1982)
Not duplicated in	Dp(2;3)17 (Roote, 1997.6.3)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{HS-DNApol-γ35.I} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}CG33649EY06366 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
DNA-directed DNA polymerase activity inferred from physical interaction with tam (Wang et al., 1997)
Biological Process
CV term Evidence References
DNA-dependent DNA replication inferred from physical interaction with tam (Wang et al., 1997) mitochondrial genome maintenance inferred from mutant phenotype (Iyengar et al., 2002) mitochondrion organization inferred from mutant phenotype (Iyengar et al., 2002)
Cellular Component
CV term Evidence References
gamma DNA polymerase complex inferred from physical interaction with tam (Wang et al., 1997) mitochondrion inferred from physical interaction with tam (Wang et al., 1997)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR002315 (FlyBase Curators et al., 2004-) DNA-directed DNA polymerase activity inferred from sequence or structural similarity (Ashburner et al., 1999) inferred from sequence or structural similarity with EMBL:AF006072 (FlyBase Genome Annotators, 2002-2003) inferred from sequence or structural similarity with UniProtKB:Q9UHN1 (Sardiello et al., 2003.6.11) non-traceable author statement (Kaguni, 1997.3.20) glycine-tRNA ligase activity inferred from electronic annotation with InterPro:IPR002315 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
DNA-dependent DNA replication inferred from sequence or structural similarity with UniProtKB:Q9UHN1 (Sardiello et al., 2003.6.11) glycyl-tRNA aminoacylation inferred from electronic annotation with InterPro:IPR002315 (FlyBase Curators et al., 2004-) mitochondrial DNA replication traceable author statement (Garesse and Vallejo, 2001)
Cellular Component
CV term Evidence References
gamma DNA polymerase complex inferred from sequence or structural similarity (Ashburner et al., 1999) inferred from sequence or structural similarity with EMBL:AF006072 (FlyBase Genome Annotators, 2002-2003) mitochondrion inferred from sequence or structural similarity with EMBL:AF006072 (FlyBase Genome Annotators, 2002-2003) inferred from sequence or structural similarity with UniProtKB:Q9UHN1 (Sardiello et al., 2003.6.11)
Sequence Ontology: Class of Gene
	gene_with_dicistronic_mRNA (FlyBase Genome Annotators, 2002-2003) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions DNApol-γ35 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 533661 DroID - A comprehensive database of gene and protein interactions. • FBgn0004407 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3772064
Orthologs
Genome-wide drosophilid orthologs	Dana\GF19722 Dere\GG10179 Dgri\GH10616 Dmoj\GI17407 Dper\GL21082 Dpse\GA17267 Dsec\GM15082 Dsim\GD22037 Dvir\GJ18087 Dwil\GK15010 Dyak\GE11405
Curated drosophilid orthologs	Dpse\GA17267
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL011352-PA Aedes aegypti (mosquito) AGAP000979-PA Anopheles gambiae (mosquito) XP_001942914.1 Acyrthosiphon pisum (pea aphid) 93165 Branchiostoma floridae (lancelet) CPIJ019085 Culex pipens (mosquito) 334148 Daphnia pulex (daphnia) ENSGALP00000005560 Gallus gallus (chicken) ISCW009886-PA Ixodes scapularis (deer tick) 228927 Lottia gigantea (owl limpet) 248273 Nematostella vectensis (starlet anemone) XP_001604498.1 Nasonia vitripennis (wasp) ENSOANP00000012787 Ornithorynchus anatinus (platypus) ENSORLP00000001050 Oryzias latipes (japanese killifish) PHUM008732-PA Pediculus humanus (human body louse) 60892 Trichoplax adhaerens (metazoa) XP_001808672.1 Tribolium castaneum (red flour beetle) ENSTNIP00000002685 Tetraodon nigroviridis (green spotted pufferfish) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10961 NV15518 LH23081 ISCW009886 FBgn0229215 FBgn0217015 FBgn0205252 FBgn0193450 FBgn0170002 JGI_V11_334148 FBgn0077280 FBgn0158677 FBgn0140151 FBgn0118097 FBgn0102489 FBgn0096729 CPIJ004445 CPIJ014245 CPIJ000403 CPIJ002712 CPIJ012199 CPIJ011030 CPIJ020311 CPIJ001414 CPIJ001413 CPIJ008198 CPIJ007232 CPIJ007250 CPIJ019085 CPIJ006332 ACYPI009296 AGAP000979 AAEL011352 PHUM344090 GLEAN_05883
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	8893 Df(2R)nap8/Dp(2;2)BG, In(2LR)Gla, wgGla-1 DNApol-γ352 8894 Df(2R)nap4/Dp(2;2)BG, In(2LR)Gla, wgGla-1 DNApol-γ352 8895 Df(2R)nap7/Dp(2;2)BG, In(2LR)Gla, wgGla-1 DNApol-γ352 5886 CyO, l(2)42Bapk81i/In(2LR)Gla, wgGla-1 DNApol-γ352 3212 Df(2L)do1, pr1 cn1/In(2LR)Gla, wgGla-1 DNApol-γ352 16718 y1 w67c23; P{EPgy2}CG33649EY06366 DNApol-γ35EY06366/CyO 4297 Df(2R)nap19, cn1 bw1/Dp(2;2)BG, In(2LR)Gla, wgGla-1 DNApol-γ352 123 w1118; In(2LR)Gla, wgGla-1 DNApol-γ352 mol35Bb-6/CyO, P{sevRas1.V12}FK1 3586 In(2L)Cy, In(2R)Cy, Cy1 Bl1 cn2/DNApol-γ351 AdhUF rds pr1 cn1
Kyoto	106920 Df(2L)do1, pr1 cn1/In(2LR)Gla, wgGla-1 DNApol-γ352 107674 Df(2R)nap19, cn1 bw1/In(2LR)Gla, Dp(2;2)BG, wgGla-1 DNApol-γ352 101512 In(2L)Cy, In(2R)Cy, Cy1 Bl1 cn2 / DNApol-γ351 AdhUF rds pr1 cn1
	More stocks available...
Genomic Clones ( 2 )
	BACR16P12 BACR30I21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 411 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH14674
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT12857 AT16112 AT23777 AT25827 AT25874 AT26405 AT26474 AT26904 GH06203 GM03048 GM26671 GM26691 LD46562 LP11513 LP14906 RE43561 RE45684 RE48983 RE50883 RE64207 RE65156 RE65688 RH02586 RH42866 RH70172 SD17380 SD25433
Other clones	63810 281626 EK092807 EK190738 EK306719 RP003002794 RP003003289 RP003005140 RP003005723 RP003007559 RP003008543 RP003008624 RP003010411 RP003011482 RP003013820 RP003013841 RP003014399 RP003015535 RP003017467 RP003020277 RP003023334 RP003029585 RP003030772 RP003031239 RP003032412 RP003033839 RP003036038 RP003036084 RP003036618 RP003036794 RP003038792 RP003041381 RP003043538 RP003046939 RP003049024 RP003050499 RP003050840 RP003053025 RP003055558 RP003055887 RP003059052 RP003061300 RP003064711 RP003067234 RP003067322 RP003068768 RP003072639 RP003073624 RP003077832 RP003078701 RP003080027 RP003080079 RP003080659 RP003081025 RP003082706 RP003082721 RP003083309 RP003083521 RP003083543 RP003084031 RP003086751 RP003087637 RP003091037 RP003091866 RP003093695 RP003096063 RP003096626 RP003098173 RP003100000 RP003100404 RP003102474 RP003103180 RP003103341 RP003103992 RP003105124 RP003106978 RP003108887 RP003109163 RP003109691 RP003111535 RP003112221 RP003115691 RP003116009 RP003116868 RP003116951 RP003119160 RP003119437 RP003124338 RP003125449 RP003126367 RP003127772 RP003129473 RP003131834 RP003132541 RP003132665 RP003133432 RP003133772 RP003135821 RP003135863 RP003136849 RP003137295 RP003137761 RP003140168 RP003140398 RP003142433 RP003143601 RP003143785 RP003147310 RP003147958 RP003148203 RP003148855 RP003148902 RP003149278 RP003149476 RP003150553 RP003150981 RP003155426 RP003159321 RP003160618 RP003161062 RP003162706 RP003162714 RP003162928 RP003163399 RP003163568 RP003166991 RP003170439 RP003170617 RP003173989 RP003174242 RP003174588 RP003175027 RP003177695 RP003179374 RP003179905 RP003180629 RP003181615 RP003182139 RP003184533 RP003185147 RP003185226 RP003185307 RP003185515 RP003186409 RP003187495 RP003187989 RP003188050 RP003191812 RP003194296 RP003197032 RP003198322 RP003201694 RP003202753 RP003203532 RP003203823 RP003204641 RP003210963 RP003211608 RP003214660 RP003215339 RP003216662 RP003219753 RP003221502 RP003223130 RP003232347 RP003236563 RP003239635 RP003240007 RP003240074 RP003241081 RP003241326 RP003246099 RP003246213 RP003246272 RP003246587 RP003251825 RP003255026 RP003256829 RP003259878 RP003267451 RP003267531 RP003275410 RP003275838 RP003278939 RP003279278 RP003283018 RP003284732 RP003285232 RP003286447 RP003288366 RP003292582 RP003295114 RP003295504 RP003297051 RP003297465 RP003299569 RP003302674 RP003305614 RP003312781 RP003320371 RP003320972 RP003323746 RP003329727 RP003334129 RP003342306 RP003345866 RP003347665 RP003349917 RP003350576 RP003352457 RP003353981 RP003354822 RP003354912 RP003361895 RP003364540 RP003365824 RP003367525 RP003368153 RP003368683 RP003369097 RP003369895 RP003371682 RP003372732 RP003374813 RP003380505 RP003380840 RP003381685 RP003383885 RP003384220 RP003385107 RP003385649 RP003386299 RP003386621 RP003388154 RP003388156 RP003390115 RP003392106 RP003392178 RP003393029 RP003393531 RP003393755 RP003394369 RP003396985 RP003397379 RP003398878 RP003399165 RP003404288 RP003407435 RP003408574 RP003409446 RP003413916 RP003414958 RP003418158 RP003425496 RP003426735 RP003426824 RP003430033 RP003430264 RP003430947 RP003432728 RP003437898 RP003438273 RP003438748 RP003441299 RP003442771 RP003448430 RP003449873 RP003451548 RP003452734 RP003453082 RP003456641 RP003456941 RP003462228 RP003464581 RP003464861 RP003465020 RP003466488 RP003468473 RP003474251 RP003475654 RP003479687 RP003483804 RP003484195 RP003485198 RP003486205 RP003487376 RP003488711 RP003492795 RP003493587 RP003494014 RP003494444 RP003494450 RP003494678 RP003494814 RP003495199 RP003495953 RP003496765 RP003498625 RP003502467 RP003505354 RP003509923 RP003510574 RP003510745 RP003512193 RP003513738 RP003516032 RP003516096 RP003516302 RP003516353 RP003525166 RP003526372 RP003527018 RP003528250 RP003530534 RP003532141 RP003532926 RP003535076 RP003535986 RP003536023 RP003536304 RP003537349 RP003544252 RP003545061 RP003545797 RP003546287 RP003547630 RP003549157 RP003553452 RP003556559 RP003557391 RP003557591 RP003558433 RP003559093 RP003561030 RP003563069 RP003563721 RP003566011 RP003566224 RP003567152 RP003569136 RP003570008 RP003571090 RP003573164 RP003576619 RP003577832 RP003577943 RP003581786 RP003585360 RP003586091 RP003588812 RP003588816 RP003589509 RP003589980 RP003593173 RP003596346 RP003597648 RP003599180 RP003601547 RP003610997 RP003612353 RP003617227 RP003622177 RP003622691 RP003623639 RP003625664 RP003627590 RP003632489 RP003633215 RP003634359 RP003640538 RP003648062 RP003648848 RP003649201 RP003649805 RP003650928 RP003658160 RP003662999 RP003663227 RP003664905 RP003667839 RP003675612 RP003678027 RP003682699
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004407 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318858 3772064
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: MtPolB l(2)34De (Ashburner et al., 1999)
Additional comments	Dicistronic annotation CG33084 split out into separate annotations for each open reading frame, CG33649 and CG33650 in release 4.2 of the genome annotation. CG33650 corresponds to DNApol-γ35. (FlyBase Genome Annotators, 2005) One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG33649 and DNApol-γ35. (FlyBase Genome Annotators, 2002-2003) The identity of l(2)34De and MtPolB is based on mapping with T(2;3)b89e12 ordering l(2)34De and RpII33. (Ashburner et al., 1999.10.12)
Other Comments
	Multiple amino acid sequence alignments show the DNA polymerases from numerous species forms a family strongly conserved from yeast to man. (Lecrenier et al., 1997) Subunit structure and enzymatic activity of DNA polymerase γ are studied using a combination of physical and immunological approaches. The role of the 2 subunits in template primer DNA binding is explored. (Olson et al., 1995) mtSSB stimulates DNA polymerase γ activity in vitro. (Williams and Kaguni, 1995) The rate and processivity of DNA synthesis by DNA polymerase γ has been analysed under a variety of different conditions. (Williams et al., 1993) Biochemical description of the 3'->5' exonuclease associated with mitochondrial DNA polymerase. (Olson and Kaguni, 1992) The 3' to 5' exonuclease encoded provides a proof reading function to enhance the fidelity of DNA synthesis during mitochondrial DNA replication. (Kaguni and Olson, 1989) A structural and mechanistic analysis of DNApol-γ125 and DNApol-γ35 demonstrates them to be both effective and accurate in the synthesis of DNA. (Kaguni et al., 1988) Comparison of the rate and specificity of DNA synthesis by DNApol-γ125 and DNApol-γ35 on several template primers, and an examination of the products of DNA synthesis has been carried out. (Wernette et al., 1988) DNApol-γ125 and DNApol-γ35 have been isolated and purified and their subunit structure and catalytic properties examined. (Wernette and Kaguni, 1986)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001032091 RefSeq (Proteins) • NP_001027262 DDBJ / EMBL / GenBank • AA695573 AAC47536 AAF44825 AAF53341 AAF53341.2 AAK92915 AAO41185.1 AE003408 AI238599 AI513318 AY051491 CL527895 L39629 U94702 Entrez Gene - A searchable database of RefSeq genes. • 3772064 UniProtKB/TrEMBL • O02005 Q9VJV8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Anticodon-binding (IPR004154) Glycyl-tRNA synthetase, alpha2 dimer (IPR002315) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME08969
Linkouts
	BioGRID - A database of protein and genetic interactions • 533661 DroID - A comprehensive database of gene and protein interactions. • FBgn0004407 DRSC - Results from RNAi screens. • FBgn0004407 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004407 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33084-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004407 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318858 3772064 InParanoid A subset of ortholog calls from InParanoid. • AAEL011352-PA Aedes aegypti (mosquito) AGAP000979-PA Anopheles gambiae (mosquito) XP_001942914.1 Acyrthosiphon pisum (pea aphid) 93165 Branchiostoma floridae (lancelet) CPIJ019085 Culex pipens (mosquito) 334148 Daphnia pulex (daphnia) ENSGALP00000005560 Gallus gallus (chicken) ISCW009886-PA Ixodes scapularis (deer tick) 228927 Lottia gigantea (owl limpet) 248273 Nematostella vectensis (starlet anemone) XP_001604498.1 Nasonia vitripennis (wasp) ENSOANP00000012787 Ornithorynchus anatinus (platypus) ENSORLP00000001050 Oryzias latipes (japanese killifish) PHUM008732-PA Pediculus humanus (human body louse) 60892 Trichoplax adhaerens (metazoa) XP_001808672.1 Tribolium castaneum (red flour beetle) ENSTNIP00000002685 Tetraodon nigroviridis (green spotted pufferfish) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3772064 modMine - Data generated by the modENCODE project. • FBgn0004407 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10961 NV15518 LH23081 ISCW009886 FBgn0229215 FBgn0217015 FBgn0205252 FBgn0193450 FBgn0170002 JGI_V11_334148 FBgn0077280 FBgn0158677 FBgn0140151 FBgn0118097 FBgn0102489 FBgn0096729 CPIJ004445 CPIJ014245 CPIJ000403 CPIJ002712 CPIJ012199 CPIJ011030 CPIJ020311 CPIJ001414 CPIJ001413 CPIJ008198 CPIJ007232 CPIJ007250 CPIJ019085 CPIJ006332 ACYPI009296 AGAP000979 AAEL011352 PHUM344090 GLEAN_05883
Synonyms & Secondary IDs ( 29 )
Reported As
Symbol Synonym	BG:DS00941.9 (Ashburner et al., 1999.10.12, Ashburner et al., 1999) br16 (Gubb et al., 1985, Ashburner and Harrington, 1984) CG8969   CG33084   CG33650   DNA pol-gam.35 (De Lucia et al., 2005) DNApol-γ (Dusenbery and Smith, 1996, Williams and Kaguni, 1995, Williams et al., 1993) DNApol-γ35 (Cayirlioglu et al., 2003) DNApol-γ.35 (De Lucia et al., 2005) D-pol γ-β (Lefai et al., 2000) l(2)34De (Iyengar et al., 2002, Pflumm and Botchan, 2001, Iyengar et al., 1999, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Roote, 1998.5.15, Alexandrova et al., 1996, Roote et al., 1996, Spradling et al., 1995, Lindsley and Zimm, 1992, Gubb et al., 1990, ) l(2)br16 (The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Gubb et al., 1984, Ashburner et al., 1982, Woodruff and Ashburner, 1979, ) MtPolB (Reese et al., 2000, Kaguni, 1997.3.20, Ashburner et al., 1999, Ashburner et al., 1999, ) PolG (Martínez-Azorín et al., 2008) polγ (Fan et al., 1999, Farr et al., 1999) Polγ (Lecrenier et al., 1997) pol γ35 (Baqri et al., 2009) pol γ-β (Luo and Kaguni, 2005, Iyengar et al., 2002, Fan and Kaguni, 2001, Lefai et al., 2000) polγ-β (Lefai et al., 2000)
Name Synonym	accessory(β)subunit (Wang and Kaguni, 1999) accessory subunit of mitochondrial DNA polymerase (Sardiello et al., 2005) DNA polymerase γ (Farr et al., 2004, Vontom and Kaguni, 1992) DNA polymerase -γ (Martínez-Azorín et al., 2008) DNA polymerase γ 35kD   lethal 16 in the black-reduced region (Woodruff and Ashburner, 1979) lethal(2)34De   Mitochondrial DNA polymerase accessory subunit   mitochondrial γ polymerase (Mandavilli et al., 2002) β subunit (Alahari et al., 1999, Wang et al., 1997)
Secondary FlyBase IDs
	FBgn0000476 FBgn0001962 FBgn0020398 FBgn0053084 FBgn0053650 FBpp0088773 FBpp0088774 FBtr0089832 FBtr0089833
References ( 65 )
Generate a list of	All references relating to this gene ( 65 )
List References by type	Research paper  ( 35 ) Supplementary material  ( 2 ) Review  ( 5 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 2 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Book  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...