odz, Tenm, odd Oz, l(3)05309, odd-Oz
transmembrane protein that acts together with the filamin Cheerio to influence growth cone progression - acts in projection neurons autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate olfactory receptor neuron active zone number
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.44
Stop-codon suppression (UGA) postulated; FBrf0216884.
gene_with_stop_codon_read_through ; SO:0000697
Gene model reviewed during 5.45
Gene model reviewed during 5.56
A non-AUG start codon may be used for translation of one or more transcripts of this gene; based on the presence of conserved protein signatures within the 5' UTR without an in-frame AUG (FBrf0243886).
11.5, 10.5 (northern blot)
None of the polypeptides share 100% sequence identity.
By Western and immunoprecipitation analyses, the Ten-m protein appears to be a large 900-1000kD secreted proteoglycan. After digestion with chondroitinase ABC, a ~270 kD core is released, whose size agrees well with the predicted molecular weight of 281 kD. Ten-m, a putative secondary pair rule gene product, is an extracellular protein.
The predicted 300 kD Ten-m protein might be post-translationally modified to give the 55 and 70 kD forms observed on Western blots. The EGF repeats of the Ten-m protein show 39% amino acid identity with the EGF repeats of mouse tenascin.
Homodimer. Heterodimer with Ten-a. Interacts with Ten-a; the interaction occurs at the neuromuscular junction. Interacts with alpha-Spec and cher.
Phosphorylated. Phosphorylation occurs at tyrosine residues.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ten-m using the Feature Mapper tool.
Comment: reference states 9.5 hr AEL
At the cellular blastoderm stage (2.5 hours), Ten-m protein is visible at the periphery of cells, suggesting a plasma-membrane associated or extracellular localization.
At embryonic stage 8, Ten-m (as well as cher), is expressed in all furrows and in the posterior midgut. It is expressed in a repetitive pattern at stage 11. By stage 13, it is epressed in the ventral nerve cord. At stage 15, Ten-m is expressed in the axons of the CNS. It is expressed in bands of epidermal cells at segmental boundaries, where it colocalizes with cher.
Ten-m is expressed posterior to the morphogenetic furrow in the third instar eye disc, and as point-like expression in more mature developing ommatidia. At higher resolutions, Ten-m can be seen in four staggered columns of preclusteres after the furrow, at the periphery of cells.
Ten-m protein is expressed in a complex pattern of the eye-antennal disc in the third instar larva. Protein is distributed in in a row of cells in the morphogenetic furrow, and in two rows posterior to the furrow; staining in these cells is uniform, and consistant with a cell surface distribution. No staining is observed for another 10 cell diameters, after which Ten-m expression is observed in a single cell, likely the R7 photoreceptor cell, of each maturing ommatidium. Expression is also observed in the optic stalk, and in two clusters of adepithelial cells, one in the eye disc and one in the antennal disc. Expression in the antennal disc is observed in the presumptive maxillary palpus, in the presumptive antennal segments, in the presumptive head capsule, and in the presumptive rostral membrane.
Ten-m protein is expressed in a complex pattern in the wing disc. In the wing pouch, it is expressed in two hook-shaped contiguous lines of expression in apposing patterns on either side of the dorsal/ventral boundary; a portion of each parallels about a quarter of the dorsal/ventral boundary at a distance of 3-4 cell lengths, then turns to follow the anterior/posterior boundary at about the location of the presumptive third longitudinal wing vein. Expression is also observed in the presumptive first, third, and fourth axillary sclerites, in the presumptive prealar apophysis, the presumptive pleural wing process, the presumptive yellow club, the presumptive pleural wing sclerite, and in the dorsal tissue giving rise to the proximal dorsal radius and the sensilla campaniformia that cover it. Expression is also observed in the presumptive proximal costal vein, in the presumptive prescutum and scutum, in the presumptive anterior and posterior notal wing processes, the presumptive tegula, the presumptive alula, the presumtive axillary cord, and in the regions of presumptive pleura adjacent to the wing.
Ten-m protein expression is strong in the regions of the haltere disc that give rise to the sensilla of the dorsal and ventral pedicel, and of dorsal and ventral scabellum. Ten-m protein is distributed in concentric rings in the leg discs, corresponding to the segments of the adult leg.
The Ten-m protein is present uniformly in the anterior-posterior axis, and in an anterior domain in stage 5 embryos, but by stage 6, a repetitive pattern comprising seven full stripes and two partial stripes are visible. By stage 8, Ten-m protein is present in the anterior and posterior transverse furrows, and in the posterior midgut primordia, with decreasing staining in the ectoderm. By stage 9, Ten-m protein is only visible in the mesoderm. At stage 10, the neuroblasts of the procephalic lobe stain for Ten-m protein, and from stage 12 to 15 Ten-m protein is visible in the axons of the ventral nerve cord. At stage 11, staining in the tracheal system becomes visible. At stages 12-13, staining is also seen in cardiac cells. At stage 16, muscle attachment sites show staining.
The 7 stripe segmentally repeated pattern of Ten-m protein expression in 2.5 to 3.5 hour embryos is followed by a 14-stripe segmentally repeated pattern of expression at 4.5 to 7.5 hours. The most intense segmentally repeated staining is seen in the mesoderm. At 9.5 hours, Ten-m protein is found in cardiac mesoderm cells, which will become cardioblasts. Also at 9.5 hours, and continuing through embryogenesis, is central nervous system staining of longitudinal and commissural axon tract bundles.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Ten-m in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Ten-m CG5723
Ten-m instructs synaptic partner matching of projection neuron dendrites to the appropriate olfactory receptor neuron axon through homophilic attraction.
Ten-m loss of function mutations do not show a pair-rule segmentation phenotype. The pair-rule phenotype previously reported for a number of Ten-m mutations has been shown to be due to a mutation present on the balancer in the original stock.
dsRNA made from templates generated with primers directed against this gene used in a cell-based RNAi assay to identify components or modifiers of the JAK/STAT pathway.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become round and detached. Kc167 cells are unaffected.
It is possible that the mutation referred to as "l(3)00844" in FBrf0082995 is actually l(3)0086400864. Ten-m is at 79E whereas the insertion in FBrf0082995 is clearly shown as mapping to between 75C5-7 and 75F1, as does l(3)0086400864.
Ten-m encodes a type I transmembrane protein with the vast C-terminal portion in the intracellular space. The polypeptide undergoes multiple cleavages at discrete intracellular and extracellular sites and its extreme C-terminus undergoes either processing at a very large number of sites or programmed degradation. The polypeptide is presented at the cell surface as two subunits of previously cleaved protein joined by cysteine disulphide bridges and it has additional post-translational modifications.
Ten-m has been characterised as a potential ligand of PS2 integrins by functional interaction in cell culture.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.
FlyBase curator comment: It is possible that the mutation referred to as "l(3)00844" is actually l(3)0086400864. Ten-m is at 79E whereas the insertion in FBrf0082995 is clearly shown as mapping to between 75C5-7 and 75F1, as does l(3)0086400864. See Roote, 2003.11.28, personal communication to FlyBase for explanation of this.
FlyBase curator comment: FBrf0214744 characterises new loss of function mutations in Ten-m and finds no evidence of a pair-rule segmentation phenotype, in contrast to previous reports. The authors show that the pair-rule phenotype previously reported for a number of Ten-m mutations (Ten-m05309, Df(3L)Ten-m-AL1 and Df(3L)Ten-m-AL29) was in fact due to a mutation present on the balancer in the original stock.
The name 'odd Oz' was suggested for this gene because of the 'odd' pair rule phenotype, and the observation that the mutant phenotype affects structures corresponding to the three gifts bestowed by the Wizard of Oz, namely heart, brain and nervous system (courage). FlyBase curator comment: FBrf0214744 has subsequently shown that loss of function Ten-m mutations do not have a pair-rule phenotype, and that the the pair-rule phenotype previously reported for a number of Ten-m mutations was in fact due to a mutation present on the balancer in the original stock.