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General Information
Symbol
Dmel\mew
Species
D. melanogaster
Name
multiple edematous wings
Annotation Symbol
CG1771
Feature Type
FlyBase ID
FBgn0004456
Gene Model Status
Stock Availability
Gene Snapshot
multiple edematous wings (mew) encodes one of 5 fly integrin alpha subunits, and when in a heterodimer with an integrin beta subunit makes a receptor for the extracellular matrix protein laminin. It is required in numerous tissues for cell migration, adhesion between cell layers, and cell differentiation. [Date last reviewed: 2019-03-14]
Also Known As
αPS1, αPS1, PS1, αPS1 integrin, PS1α
Key Links
Genomic Location
Cytogenetic map
Sequence location
X:13,214,409..13,258,490 [-]
Recombination map
1-42
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the integrin alpha chain family. (Q24247)
Molecular Function (GO)
[Detailed GO annotations]
Summaries
Gene Group (FlyBase)
INTEGRINS -
Integrins are heterodimeric transmembrane receptors composed of an α and β subunit that mediate cell-cell and cell-extracellular matrix adhesion. As well as maintaining tissue integrity, they are involved in morphogenesis and development. (Adapted from FBrf0167428).
Protein Function (UniProtKB)
Integrin alpha-PS1/beta-PS is a receptor for laminin.
(UniProt, Q24247)
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
6

Please see the GBrowse view of Dmel\mew or the JBrowse view of Dmel\mew for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Stop-codon suppression (UAA) postulated; FBrf0216884.
Gene model reviewed during 5.43
Shares 5' UTR with upstream gene.
gene_with_stop_codon_read_through ; SO:0000697
Gene model reviewed during 5.44
Gene model reviewed during 5.50
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0073731
3863
1049
FBtr0073732
5001
1146
FBtr0310647
4281
1155
FBtr0310648
4350
1058
FBtr0330398
3863
1146
FBtr0340190
4254
1243
Additional Transcript Data and Comments
Reported size (kB)
5.4 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0073562
117.1
1049
5.77
FBpp0073563
128.0
1146
6.27
FBpp0302767
129.0
1155
6.31
FBpp0302768
118.1
1058
5.84
FBpp0303424
128.3
1146
6.46
FBpp0309167
139.2
1243
6.77
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
1146 (aa); 128 (kD predicted)
Comments
External Data
Subunit Structure (UniProtKB)
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-PS1 associates with beta-PS.
(UniProt, Q24247)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mew using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (29 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (0 terms)
Biological Process (20 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from expression pattern
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Grip; FB:FBgn0029830
inferred from genetic interaction with FLYBASE:kon; FB:FBgn0032683
Terms Based on Predictions or Assertions (9 terms)
CV Term
Evidence
References
Cellular Component (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
non-traceable author statement
inferred from sequence or structural similarity with HGNC:6139
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Transcript was detected in stage 11 salivary gland primordia coincident with salivary gland internalization. Expression continued in all cells through stage 12 with high expression in invaginating cells. Transcritp was no longer detectable at stage 14. mew is also expressed in the invaginating tracheal pits.
mew transcripts are first detected at the cellular blastoderm stage in the cells of the presumptive ectoderm. During gastrulation, the transcript is apparent over the entire ectoderm with increased levels in the dorsal region. In stage 8, high transcript levels are observed in the ectoderm and in the anterior and posterior midgut primordia. By stage 11, a strong segmentally repeated pattern has been established in the ectoderm and the CNS. The regions of high concentration are correlated with segment boundaries. Strong mew expression is also detected in the dorsal compartment of the wing disc as well as in adepithelial cells and on the peripodial membrane.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
The α1 integrin, mew, strongly accumulates on the basal membrane of esg-positive cells contacting ECM, but is barely detected in any other regions along the interface between the muscle cells and the differentiated epithelial cells.
In stage 15 embryos, before the leading processes of the cardioblasts make contact across the midline, mew is localised primarily on the leading edge of the migrating cardioblasts, and less so on the trailing edge. This preponderantly apical distribution was not significantly altered once the heart lumen was formed at stage 17.
mew staining is observed along lateral, apical, and basal follicle cell membranes through stage 10A. Apical staining peaks in stage 9 and 10A columnal follicle cells overlying the oocyte while downregulating in the most posterior follicle cells. Expression is also seen in border cells at intercellular junctions. After stage 10A, the remaining follicle cell populations begin down-regulating apically localized mys while maintaining lateral and basal localization during dorsal appendage morphogenesis. Staining is observed from stage 9 in dorsal appendage forming follicle cells.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mew in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 34 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mew
Transgenic constructs containing regulatory region of mew
Deletions and Duplications ( 4 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
11 of 15
No
Yes
9 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
11 of 15
Yes
Yes
9 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
9 of 13
Yes
Yes
7 of 13
No
Yes
5 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
7 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (6)
12 of 15
Yes
Yes
9 of 15
No
Yes
8 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
10 of 15
Yes
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919014N )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500RR )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00LS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00KK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02JI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
3 of 10
2 of 10
2 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-PS1 associates with beta-PS.
    (UniProt, Q24247 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-42
    Cytogenetic map
    Sequence location
    X:13,214,409..13,258,490 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    11E3-11E8
    Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640EP1626
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    11E-11F
    (determined by in situ hybridisation) 11E1--4 (determined by in situ hybridisation) 11D1--2 (determined by in situ hybridisation)
    11E-11F
    (determined by in situ hybridisation)
    11E1-11E4
    (determined by in situ hybridisation)
    11D7-11E4
    (determined by in situ hybridisation)
    11D7-11E5
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (28)
    Genomic Clones (21)
    cDNA Clones (25)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: mew CG1771
      Source for database merge of
      Source for merge of: mew l(1)G0443 l(1)G0429
      Additional comments
      Other Comments
      One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
      Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.
      mew and scb are required in a partially redundant way in the endoderm for normal migration of the endodermal midgut cells over the visceral mesoderm in the developing embryo.
      The functional significance of the cytoplasmic domains of the if, mew and mys subunits of the Position Specific (PS) integrin family are studied by analysing the relationship between the cytoplasmic domain structure and function in the context of a developing organism. The cytoplasmic tail of mew is not required for function in the wing and in the retina.
      mew can mediate adhesion of the epidermal hemiadherens junctions to the basement membrane at muscle attachment sites in the developing embryo. This function appears to be mediated by LanA.
      The ability of two different integrin α subunits (encoded by mew and if) to substitute for each other during embryonic development has been studied. The two α subunits encoded by mew and if are not equivalent and have distinct functions which reside in the extracellular domains.
      Clonal analysis demonstrates that the different integrins, mew and if, are required on opposite wing epithelia. An early integrin-dependent process, not obviously required for prepupal adhesion, is essential to permit subsequent wing morphogenesis.
      Loss of mew produces disorganisation along the basal surface of the retina. if function can replace mew function in the retina.
      Phenotypic analysis of mew, if and mys embryos suggests multiple roles for PS integrins in the adhesion of cells and in the formation, organization and migration of embryonic tissues. Although the proteins are often expressed in adjacent embryonic tissues, this distribution does not necessarily reflect equivalent requirements. The complete loss of both α subunits, encoded by mew and if, does not produce all the phenotypes observed in embryos lacking the mys encoded β subunit.
      mew gene product promotes cell spreading on two different extra cellular matrix (ECM) molecules, laminin and tiggin.
      Origin and Etymology
      Discoverer
      Etymology
      The chosen gene symbol, "mew", will commemorate the name of the late Michael E. Wilcox, who made many important contributions to this field.
      Identification
      External Crossreferences and Linkouts ( 68 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (37)
      Reported As
      Symbol Synonym
      alpha-PS1
      Secondary FlyBase IDs
      • FBgn0028276
      • FBgn0040161
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (263)