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Gene Dmel\Mdr65

General Information
Symbol	Dmel\Mdr65	Species	D. melanogaster
Name	Multiple drug resistance 65	Annotation symbol	CG10181
Feature type	protein_coding_gene	FlyBase ID	FBgn0004513
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	65A10-65A10	Sequence location	3L:6,233,600..6,238,846 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Multiple drug resistance 65 is referred to in FlyBase by the symbol Dmel\Mdr65 (CG10181, FBgn0004513). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: drug transmembrane transporter activity. There is experimental evidence that it is involved in the biological process: establishment of blood-brain barrier; response to methotrexate. 8 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: AAA+ ATPase domain; ABC transporter, conserved site; ABC transporter, integral membrane type 1; ABC transporter, transmembrane domain; ABC transporter, transmembrane domain, type 1; ABC transporter-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult Malpighian tubules, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1631925_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Mdr65. Gene sequence location is 3L:6233600..6238846.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Controlled Vocabulary Terms establishment of glial blood-brain barrier efflux transmembrane transporter activity xenobiotic-transporting ATPase activity xenobiotic transport pleated septate junction
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 65A10-65A10   Limits computationally determined from genome sequence between P{PZ}l(3)0209402094 and P{lacW}l(3)L4060L4060
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mdr65 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Low-frequency RNA-seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mdr65-RA FBtr0077011  NM_057483   4135   1302
Additional Transcript Data & Comments
Reported size (kB)	4.124 (longest cDNA) (Wu et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mdr65-PA   FBpp0076719   143.8   1302   6.44     NP_476831   AAF50669
Additional Polypeptide Data & Comments
Reported size (kDa)	1302 (aa) (Wu et al., 1991)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ABC transporter, transmembrane domain (IPR001140) ABC transporter-like (IPR003439) AAA+ ATPase domain (IPR003593) ABC transporter, transmembrane domain, type 1 (IPR011527) ABC transporter, conserved site (IPR017871) ABC transporter, integral membrane type 1 (IPR017940) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50669   AC004357   AC004767   AF251286 AAF69146   AF251287 AAF69147   AY121669 AAM51996   BH901069   BI172318   BI232059   BI243725   BI614835   CO266959   CO302378   EC062606   EC073091   FE426529   FE862737   FE863174   FE863205   FE863323   GH740688   GH740956   GH744589   GH767970   M59077 AAA28680
UniProtKB/Swiss-Prot	Q00748
UniProtKB/TrEMBL	Q8MRC4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13 -- 16   embryonic central brain glial cell   (Fisher et al., 2012) embryonic central brain surface glia   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord surface glial cell   (Fisher et al., 2012) ventral midline   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
ATP-binding cassette (ABC) transporter complex inferred from direct assay (Mayer et al., 2009) plasma membrane inferred from direct assay (Khanna et al., 2010) pleated septate junction inferred from direct assay (Mayer et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult Malpighian tubules, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    735.05 Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (735.05) Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    735.05 Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    735.05 Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    735.05 Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  735.05 Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    735.05 Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    735.05 Larval Midgut    2.6 Larval Hindgut    7.8 Larval Malpighian Tubules    3.2 Larval Fat Body    3 Larval Salivary Gland    4.3 Larval Trachea    8.15 Larval Carcass    30.525 Adult Head    187.5 Adult Eye    168.575 Adult Brain    437.9 Adult Thoracic-Abdominal Ganglion    479.1 Adult Crop    19.4 Adult Midgut    3.9 Adult Hindgut    55.4 Adult Malpighian Tubules    272.7 Adult Fat Body    5.2 Adult Salivary Gland    3.4 Adult Heart    4.6 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.3 Adult Ovary    4.2 Adult Testis    0.6 Adult Male Accessory Gland    254.7 Adult Carcass    25 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    187.5   NA  Eye    168.575   NA  Brain    437.9   735.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    479.1   NA  Crop    19.4   2.6  Midgut    3.9   7.8  Hindgut    55.4   3.2  Malpighian Tubules    272.7   3  Fat Body    5.2   4.3  Salivary Gland    3.4   NA  Heart    4.6   8.15  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    4.2   NA  Testis    0.6   NA  Male Accessory Gland    254.7   30.525  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8    38 head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8  (38) head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8    38 head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8    38 head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8    38 head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8  (38) head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8    38 head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    11 central nervous system, pupae P8    38 head, virgin 1-day female    14 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    13 head, mated 4-day male    10 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    5 carcass, larvae L3 wandering    3 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr  26 embryo 22-24hr  (34) larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr  26 embryo 22-24hr  (34) larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    3 embryo 16-18hr    11 embryo 18-20hr    17 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    25 larva L2    11 larva L3 12hr old    6 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    8 pupae 3d postWPP    11 pupae 4d postWPP    8 adult male 01day    7 adult male 05day    4 adult male 30day    3 adult female 01day    4 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_18 mE1_20_mRNA_expression_cluster_11
External Data & Images
Linkouts	BDGP expression data • RE14657 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004513 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10181-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Mdr65 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Mdr65GD2330 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Mdr65 Class Mutagen Stocks Known lesion Mdr65KG08723 P-element activity 1 Yes Mdr65MI00104 1 -- Mdr65Ex8 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mdr65 Class Mutagen Stocks Known lesion Mdr65GD2330 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mdr65HMS01449 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mdr65JF03079 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mdr65G1190D.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Mdr65NIG.10181R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6108 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2330} NA P{NIG.10181R} NA P{TRiP.HMS01449} NA P{TRiP.JF03079} NA P{UAS-Mdr65-GFP.G1190D} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}Mdr65MI00104 NA P{SUPor-P}Mdr65KG08723 NA
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
efflux transmembrane transporter activity inferred from direct assay (Mayer et al., 2009) xenobiotic-transporting ATPase activity inferred from direct assay (Mayer et al., 2009)
Biological Process
CV term Evidence References
establishment of glial blood-brain barrier inferred from direct assay (Mayer et al., 2009) response to methotrexate inferred from expression pattern (Chahine and O'Donnell, 2009) xenobiotic transport inferred from direct assay (Mayer et al., 2009)
Cellular Component
CV term Evidence References
ATP-binding cassette (ABC) transporter complex inferred from direct assay (Mayer et al., 2009) plasma membrane inferred from direct assay (Khanna et al., 2010) pleated septate junction inferred from direct assay (Mayer et al., 2009)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
ATPase activity, coupled to transmembrane movement of substances inferred from biological aspect of ancestor with PANTHER:PTN000657188 (assigned by RefGenome) (Gaudet et al., 2010) ATP binding inferred from electronic annotation with InterPro:IPR001140, InterPro:IPR003439, InterPro:IPR011527, InterPro:IPR017871 (FlyBase Curators et al., 2004-) drug transmembrane transporter activity inferred from sequence or structural similarity with UniProtKB:P08183 (FlyBase, 1992-)
Biological Process
CV term Evidence References
transmembrane transport inferred from biological aspect of ancestor with PANTHER:PTN000657188 (assigned by RefGenome) (Gaudet et al., 2010)
Cellular Component
CV term Evidence References
integral to membrane inferred from biological aspect of ancestor with PANTHER:PTN000657188 (assigned by RefGenome) (Gaudet et al., 2010) integral to plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1993.4.1) plasma membrane inferred from biological aspect of ancestor with PANTHER:PTN000657191 (assigned by RefGenome) (Gaudet et al., 2010)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mdr65 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64179 DroID - A comprehensive database of gene and protein interactions. • FBgn0004513 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38726
Orthologs
OrthoDB Orthologs (85) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6PZJRX Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG69KDKD Arthropod inclusive ortholog search EOG6H189K Metazoa inclusive ortholog search EOG6VQPFZ
Orthologs in Drosophila Species (EOG6PZJRX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Mdr65     Drosophila simulans   Dsim\GD13132     Drosophila sechellia   Dsec\GM13846     Drosophila erecta   Dere\GG14065     Drosophila yakuba   Dyak\GE20489     Drosophila ananassae   Dana\GF10905     Drosophila pseudoobscura pseudoobscura   Dpse\GA10136     Drosophila persimilis   Dper\GL22523     Drosophila willistoni   Dwil\GK13588     Drosophila virilis   Dvir\GJ12000     Drosophila mojavensis   Dmoj\GI13225     Drosophila grimshawi   Dgri\GH23394     Drosophila grimshawi   Dgri\GH16282
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG69KDKD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB55378 Y Apis mellifera Western honey bee  Amel\Ame.5659 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG021290 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10590 Y Atta cephalotes Leafcutter ant  Acep\ACEP23211 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO21228 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17540 Y Linepithema humile Argentine ant  Lhum\LH12477 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB11766 Y Solenopsis invicta Red fire ant  Sinv\SINV18862 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000724 Y Bombyx mori Silkmoth  Bmor\BGIBMGA009452 Y Bombyx mori Silkmoth  Bmor\BGIBMGA007494 Y Bombyx mori Silkmoth  Bmor\BGIBMGA011228 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000725 Y Pediculus humanus Human body louse  Phum\PHUM261630 Y Pediculus humanus Human body louse  Phum\PHUM578820 Y Pediculus humanus Human body louse  Phum\PHUM177140 Y Tribolium castaneum Red flour beetle  Tcas\TC003797 Y Tribolium castaneum Red flour beetle  Tcas\TC003402 Y
Orthologs in non-Insect Arthropods (EOG6H189K)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Prdm8 Y Daphnia pulex Water flea  Dpux\COI Y Ixodes scapularis Deer tick  Isca\ISCW017811 Y Ixodes scapularis Deer tick  Isca\ISCW004310 Y
Orthologs in non-Arthropod Metazoa (EOG6VQPFZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\pgp-6 Y Caenorhabditis elegans Nematode  Cele\pgp-1 Y Caenorhabditis elegans Nematode  Cele\pgp-5 Y Caenorhabditis elegans Nematode  Cele\pgp-8 Y Caenorhabditis elegans Nematode  Cele\pgp-3 Y Caenorhabditis elegans Nematode  Cele\pgp-4 Y Caenorhabditis elegans Nematode  Cele\pgp-12 Y Caenorhabditis elegans Nematode  Cele\pgp-14 Y Caenorhabditis elegans Nematode  Cele\pgp-13 Y Caenorhabditis elegans Nematode  Cele\pgp-2 Y Caenorhabditis elegans Nematode  Cele\pgp-7 Y Caenorhabditis elegans Nematode  Cele\pgp-9 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_002431gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019185gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007015gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008443gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027647gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007014gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001752gn Y Danio rerio Zebrafish  Drer\BX004890.1 Y Danio rerio Zebrafish  Drer\abcb4 Y Danio rerio Zebrafish  Drer\ENSDARG00000075665 Y Danio rerio Zebrafish  Drer\abcb11a Y Danio rerio Zebrafish  Drer\abcb5 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033201 Y Xenopus tropicalis Western clawed frog  Xtro\abcb11 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033417 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000023590 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000007433 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000005990 Y Xenopus tropicalis Western clawed frog  Xtro\abcb1 Y Gallus gallus Domestic chicken  Ggal\ABCB11 Y Gallus gallus Domestic chicken  Ggal\ABCB1 Y Gallus gallus Domestic chicken  Ggal\ABCB1 Y Gallus gallus Domestic chicken  Ggal\LOC420606 Y Mus musculus House mouse  Mmus\Abcb4 Y Mus musculus House mouse  Mmus\Abcb5 Y Mus musculus House mouse  Mmus\Abcb11 Y Mus musculus House mouse  Mmus\Abcb1b Y Mus musculus House mouse  Mmus\Abcb1a Y Rattus norvegicus Norway rat  Rnor\Abcb1b Y Rattus norvegicus Norway rat  Rnor\Abcb5 Y Rattus norvegicus Norway rat  Rnor\Abcb1a Y Rattus norvegicus Norway rat  Rnor\Abcb4 Y Homo sapiens Human  Hsap\ABCB11 Y Homo sapiens Human  Hsap\ABCB1 Y Homo sapiens Human  Hsap\ENSG00000004846 Y Homo sapiens Human  Hsap\ENSG00000005471 Y
Human Orthologs (4)
Gene OMIM HGNC Hsap\ABCB11     Hsap\ABCB1     Hsap\ENSG00000004846     Hsap\ENSG00000005471
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	28664 y1 v1; P{TRiP.JF03079}attP2 30619 y1 w*; Mi{MIC}Mdr65MI00104 35035 y1 sc* v1; P{TRiP.HMS01449}attP2 14757 y1 w67c23; P{SUPor-P}Mdr65KG08723 ry506
VDRC	v9019 w1118; P{GD2330}v9019
Genomic Clones ( 2 )
	BACR09G21 BACR30A03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 17 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE14657
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE28065 RE41444 RH44315
Other clones	36871 55906 EK148030 EK184029 RM05364 RM06064 RM06264 RM06464 RM06564 RP001010115 RP001019116 RP001040275 RP001052959
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004513 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38726
Antibody Information
	polyclonal (Mayer et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Mdr65 CG10181 (Gabrielian, 1999.11)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057483 RefSeq (Proteins) • NP_476831 DDBJ / EMBL / GenBank • AAA28680 AAF50669 AAF69146 AAF69147 AAM51996 AC004357 AC004767 AF251286 AF251287 AY121669 BH901069 BI172318 M59077 Entrez Gene - A searchable database of RefSeq genes. • 38726 UniProtKB/Swiss-Prot • Q00748 UniProtKB/TrEMBL • Q8MRC4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ABC transporter, transmembrane domain (IPR001140) ABC transporter-like (IPR003439) AAA+ ATPase domain (IPR003593) ABC transporter, transmembrane domain, type 1 (IPR011527) ABC transporter, conserved site (IPR017871) ABC transporter, integral membrane type 1 (IPR017940) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE14657
Linkouts
	BioGRID - A database of protein and genetic interactions • 64179 DroID - A comprehensive database of gene and protein interactions. • FBgn0004513 DRSC - Results from RNAi screens. • FBgn0004513 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004513 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10181-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004513 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38726 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38726 modMine - Data generated by the modENCODE project. • FBgn0004513
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG10181 (Perkins et al., 2009, Khanna et al., 2010, Chahine and O'Donnell, 2009, Ni et al., 2011.7.19) Mdr65 (Mayer et al., 2009, Japanese National Institute of Genetics, 2012.5.21) mdr65 (Ricardo and Lehmann, 2009, Ricardo and Lehmann, 2009) MDR65 (Chahine and O'Donnell, 2009) Mdr65A (Begun and Whitley, 2000)
Name Synonym	Multiple drug resistance 65 (Khanna et al., 2010) P-glycoprotein65-Dm (Nasonkin et al., 1999)
Secondary FlyBase IDs
References ( 43 )
Generate a list of	All references relating to this gene ( 43 )
List References by type	Research paper  ( 13 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 3 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 2 )
Recent research papers ( 1 )
	Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...