l(3)64Ah, cs1, Unc18
Gene model reviewed during 5.43
Gene model reviewed during 5.45
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rop using the Feature Mapper tool.
Comment: reported as dorsal/lateral sensory complexes
Rop transcripts are expressed in the garland cells starting in stage 13 of embryogenesis. They are also observed in the CNS, the PNS, and the antenno-maxillary complex. The pattern is very similar to that of Ras64B. The probe used does not distinguish between the two transcripts.
Fractionation results show that Rop protein is primarily in the cytosol but it is also peripherally associated with the plasma membrane.
Rop protein is detected throughout the embryo at low levels until stage 13, after which is appears at higher levels in some tissues. These include the epidermis, garland cells, gut, Malpighian tubules, salivary glands, and eventually in the axon tracts of the CNS and in the segmental and intersegmental nerves. In third instar larvae, Rop protein is found at high levels in the neuropil of the CNS, in the axon bundles and in their synapse rich termini (boutons). It is most abundant in the periphery of the boutons. Rop protein is observed at lower levels in the imaginal discs and muscles.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Rop in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The Rop gene product plays a role in general secretion and is required for exocytosis from a variety of secretory tissues.
Rop is a member of the "Sec1" family of proteins involved in synaptic transmission and general secretion.
The Rop protein plays a negative role in neurotransmitter release, in addition to its previously identified positive function possible by modulation of docking of synaptic vesicles or activation of a pre-fusion complex at the active zone.
Rop plays a positive role in synaptic transmission and functions in both secretion and neurotransmission.
A 3-5 fold induction of Rop protein in third instar larvae causes a dramatic decrease in neurotransmitter release. The number of spontaneous vesicle fusions is reduced by more than 50%, though calcium dependence of neurotransmitter release is not significantly altered.
Rop plays a negative role in neurotransmitter release in vivo possibly by modulating the docking of synaptic vesicles or activation of a pre-fusion complex at the active zone.
Rop is divergently transcribed from Ras64B, and the two transcription units are only 93 bases apart. Based on sequence similarities with related interacting proteins in yeast, it is suggested that Rop and Ras64B gene products are not simply coexpressed, but may interact with each other.