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Gene Dmel\Rop

General Information
Symbol	Dmel\Rop	Species	D. melanogaster
Name	Ras opposite	Annotation symbol	CG15811
Feature type	protein_coding_gene	FlyBase ID	FBgn0004574
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	l(3)64Ah
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	64A7-64A7	Sequence location	3L:4,136,359..4,138,996 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ras opposite is referred to in FlyBase by the symbol Dmel\Rop (CG15811, FBgn0004574). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: SNARE binding. There is experimental evidence that it is involved in the biological process: synaptic transmission; response to light stimulus; neurotransmitter secretion; cytokinesis; regulation of pole plasm oskar mRNA localization; secretion by cell. 24 alleles are reported. The phenotypes of these alleles are annotated with: tracheal branch primordium; embryonic/first instar larval cuticle; central nervous system. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Sec1-like protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627197_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rop. Gene sequence location is 3L:4136359..4138996.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64A7-64A7   Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64A10-64A11   (determined by in situ hybridisation)   (Harrison et al., 1995) 64A-64B   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rop for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rop-RA FBtr0073263  NM_079192   2209   597 Rop-RB FBtr0310632  NM_001274475   2638   597
Additional Transcript Data & Comments
Reported size (kB)	2.9, 2.5 (northern blot) (Salzberg et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rop-PA   FBpp0073119   67.9   597   6.67     NP_523916   AAF47844 Rop-PB   FBpp0302752   67.9   597   6.67     NP_001261404   AGB94099
Additional Polypeptide Data & Comments
Reported size (kDa)	597 (aa); 68 (kD observed); 68 (kD predicted) (Salzberg et al., 1993) 75 (kD observed) (Harrison et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sec1-like protein (IPR001619) Sec1-like, domain 2 (IPR027482)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390839   AE014296 AAF47844   AE014296 AGB94099   AI062728   AI062798   AI108137   AI260783   AI294379   AI388787   AI513854   AI530843   AI532637   AI533375   AI533653   AI542107   AI542197   AY052094 AAK93518   BF485827   BF491728   BF494423   BG635837   BG636368   BG640216   BI165562   BI167341   BI173724   BI212866   BI216656   BI232292   BI234728   BI239952   BI352310   BI353159   BI361833   BI362432   BI363015   BI366942   BI371482   BI481720   BI485841   BI563591   BI563846   BI566136   BI566903   BI568180   BI568471   BI568602   BI584884   BI585728   BI605644   BI609639   BI610894   BI611559   BI617217   BI617548   BI624188   BI627863   BI628769   BI630648   BI630936   BI634645   BI636018   BI636343   BI637036   BI637056   BI637973   BI638281   BI638840   BP541799   CA806256   CO153126   CO155299   CO155817   CO156791   CO179453   CO187050   CO187325   CO262559   CO262841   CO262946   CO264956   CO265437   CO266372   CO267645   CO267940   CO269141   CO270665   CO277495   CO277999   CO280407   CO285252   CO286744   CO288268   CO291430   CO293757   CO294113   CO296759   CO298230   CO304052   CO306442   CO309024   CO309075   CO312171   CO312247   CO316866   CO319210   CO322861   CO337274   CO338366   CO340362   CO340415   CO340505   EC058994   EC059530   EC063891   EC066314   EC068295   EC068985   EC070139   EC071174   EC071768   EC075292   EC078559   EC080906   EC083416   EC089438   EC196767   EC202226   EC203168   EC203360   EC208491   EC208730   EC213536   EC215981   EC216801   EC218827   EC219520   EC224347   EC229483   EC236729   EC240199   EC245721   EC247672   EC249632   EC252071   EC260336   EC262665   EC263494   EC264028   EL883220   EL883453   U15967 AAB60242   X67218 CAA47658   X67219 CAA47659
UniProtKB/Swiss-Prot	Q07327
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval peripheral nervous system   (Salzberg et al., 1993) garland cell   (Salzberg et al., 1993) central nervous system   (Salzberg et al., 1993) antenno-maxillary complex   (Salzberg et al., 1993) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:localized, maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) garland cell primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic antennal sense organ   (Fisher et al., 2012) embryonic brain   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic/larval garland cell   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012)
Additional Descriptive Data
	Rop transcripts are expressed in the garland cells starting in stage 13 of embryogenesis. They are also observed in the CNS, the PNS, and the antenno-maxillary complex. The pattern is very similar to that of Ras64B. The probe used does not distinguish between the two transcripts. (Salzberg et al., 1993)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system   (Harrison et al., 1994) embryonic stage 13 -- 17   epidermis   (Harrison et al., 1994) embryonic stage 14 -- 17   embryonic/larval salivary gland   (Harrison et al., 1994) embryonic/larval digestive system   (Harrison et al., 1994) garland cell   (Harrison et al., 1994) Malpighian tubule   (Harrison et al., 1994) embryonic stage 16 -- 17   intersegmental nerve   (Harrison et al., 1994) segmental nerve   (Harrison et al., 1994) third instar larval stage   imaginal disc   (Harrison et al., 1994) intersegmental nerve   (Harrison et al., 1994) Bolwig nerve   (Harrison et al., 1994) embryonic/larval muscle system   (Harrison et al., 1994) segmental nerve   (Harrison et al., 1994) bouton   (Harrison et al., 1994)
Additional Descriptive Data
	Rop protein is detected throughout the embryo at low levels until stage 13, after which is appears at higher levels in some tissues. These include the epidermis, garland cells, gut, Malpighian tubules, salivary glands, and eventually in the axon tracts of the CNS and in the segmental and intersegmental nerves. In third instar larvae, Rop protein is found at high levels in the neuropil of the CNS, in the axon bundles and in their synapse rich termini (boutons). It is most abundant in the periphery of the boutons. Rop protein is observed at lower levels in the imaginal discs and muscles. (Harrison et al., 1994)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytosol inferred from direct assay (Harrison et al., 1994) extrinsic to membrane inferred from direct assay (Harrison et al., 1994) terminal bouton inferred from direct assay (Harrison et al., 1994)
Expression Deduced from Reporters
Reporter: P{lacZRop.P} Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval salivary gland   (Salzberg et al., 1993) embryonic/larval central nervous system   (Salzberg et al., 1993) adult stage & oogenesis   oocyte   (Salzberg et al., 1993) nurse cell   (Salzberg et al., 1993) follicle cell   (Salzberg et al., 1993) oogenesis & adult stage | female   ovary   (Salzberg et al., 1993) spermatogenesis & adult stage   testis   (Salzberg et al., 1993) cyst cell   (Salzberg et al., 1993)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain    750 Adult Thoracic-Abdominal Ganglion    879.6 Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain  (750) Adult Thoracic-Abdominal Ganglion  (879.6) Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain    750 Adult Thoracic-Abdominal Ganglion    879.6 Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain    750 Adult Thoracic-Abdominal Ganglion    879.6 Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain    750 Adult Thoracic-Abdominal Ganglion    879.6 Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain  750 Adult Thoracic-Abdominal Ganglion  (879.6) Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain    750 Adult Thoracic-Abdominal Ganglion    879.6 Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    221.825 Larval Midgut    202.2 Larval Hindgut    307.7 Larval Malpighian Tubules    400.7 Larval Fat Body    127.8 Larval Salivary Gland    321.2 Larval Trachea    169.475 Larval Carcass    160.85 Adult Head    338.3 Adult Eye    370.575 Adult Brain    750 Adult Thoracic-Abdominal Ganglion    879.6 Adult Crop    173.1 Adult Midgut    257.7 Adult Hindgut    257.6 Adult Malpighian Tubules    292.5 Adult Fat Body    91.3 Adult Salivary Gland    202.4 Adult Heart    197.55 Adult VirginFemale Spermatheca    179.4 Adult InseminatedFemale Spermatheca    191.6 Adult Ovary    147.5 Adult Testis    104.7 Adult Male Accessory Gland    107 Adult Carcass    124.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    338.3   NA  Eye    370.575   NA  Brain    750   221.825  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    879.6   NA  Crop    173.1   202.2  Midgut    257.7   307.7  Hindgut    257.6   400.7  Malpighian Tubules    292.5   127.8  Fat Body    91.3   321.2  Salivary Gland    202.4   NA  Heart    197.55   169.475  Trachea    NA   NA  VirginFemale Spermatheca    179.4   NA  InseminatedFemale Spermatheca    191.6   NA  Ovary    147.5   NA  Testis    104.7   NA  Male Accessory Gland    107   160.85  Carcass    124.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    39 central nervous system, pupae P8    150 head, virgin 1-day female    105 head, virgin 4-day female    72 head, virgin 20-day female    57 head, mated 1-day female    67 head, mated 4-day female    60 head, mated 20-day female    42 head, mated 1-day male    157 head, mated 4-day male    119 head, mated 20-day male    102 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    39 digestive system, larvae L3 wandering    52 digestive system, 1-day adult    38 digestive system, 4-day adult    29 digestive system, 20-day adult    44 fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8    40 carcass, larvae L3 wandering    32 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male    38 accessory gland, mated 4-day male    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (32) central nervous system, larvae L3  (39) central nervous system, pupae P8  (150) head, virgin 1-day female  (105) head, virgin 4-day female  (72) head, virgin 20-day female  (57) head, mated 1-day female  (67) head, mated 4-day female  (60) head, mated 20-day female  (42) head, mated 1-day male  (157) head, mated 4-day male  (119) head, mated 20-day male  (102) salivary gland, larvae L3 wandering    13 salivary gland, white prepupae  (39) digestive system, larvae L3 wandering  (52) digestive system, 1-day adult  (38) digestive system, 4-day adult  (29) digestive system, 20-day adult  (44) fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8  (40) carcass, larvae L3 wandering  (32) carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male  (38) accessory gland, mated 4-day male    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    39 central nervous system, pupae P8  (150) head, virgin 1-day female  105 head, virgin 4-day female    72 head, virgin 20-day female    57 head, mated 1-day female    67 head, mated 4-day female    60 head, mated 20-day female    42 head, mated 1-day male  (157) head, mated 4-day male  (119) head, mated 20-day male  102 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    39 digestive system, larvae L3 wandering    52 digestive system, 1-day adult    38 digestive system, 4-day adult    29 digestive system, 20-day adult    44 fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8    40 carcass, larvae L3 wandering    32 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male    38 accessory gland, mated 4-day male    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    39 central nervous system, pupae P8    150 head, virgin 1-day female    105 head, virgin 4-day female    72 head, virgin 20-day female    57 head, mated 1-day female    67 head, mated 4-day female    60 head, mated 20-day female    42 head, mated 1-day male    157 head, mated 4-day male    119 head, mated 20-day male    102 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    39 digestive system, larvae L3 wandering    52 digestive system, 1-day adult    38 digestive system, 4-day adult    29 digestive system, 20-day adult    44 fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8    40 carcass, larvae L3 wandering    32 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male    38 accessory gland, mated 4-day male    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    39 central nervous system, pupae P8    150 head, virgin 1-day female    105 head, virgin 4-day female    72 head, virgin 20-day female    57 head, mated 1-day female    67 head, mated 4-day female    60 head, mated 20-day female    42 head, mated 1-day male    157 head, mated 4-day male    119 head, mated 20-day male    102 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    39 digestive system, larvae L3 wandering    52 digestive system, 1-day adult    38 digestive system, 4-day adult    29 digestive system, 20-day adult    44 fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8    40 carcass, larvae L3 wandering    32 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male    38 accessory gland, mated 4-day male    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  32 central nervous system, larvae L3  (39) central nervous system, pupae P8  (150) head, virgin 1-day female  (105) head, virgin 4-day female  (72) head, virgin 20-day female  (57) head, mated 1-day female  (67) head, mated 4-day female  (60) head, mated 20-day female  (42) head, mated 1-day male  (157) head, mated 4-day male  (119) head, mated 20-day male  (102) salivary gland, larvae L3 wandering    13 salivary gland, white prepupae  (39) digestive system, larvae L3 wandering  (52) digestive system, 1-day adult  (38) digestive system, 4-day adult  29 digestive system, 20-day adult  (44) fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8  (40) carcass, larvae L3 wandering  32 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male  (38) accessory gland, mated 4-day male    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    39 central nervous system, pupae P8  (150) head, virgin 1-day female  105 head, virgin 4-day female    72 head, virgin 20-day female    57 head, mated 1-day female    67 head, mated 4-day female    60 head, mated 20-day female    42 head, mated 1-day male  (157) head, mated 4-day male  119 head, mated 20-day male  102 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    39 digestive system, larvae L3 wandering    52 digestive system, 1-day adult    38 digestive system, 4-day adult    29 digestive system, 20-day adult    44 fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8    40 carcass, larvae L3 wandering    32 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male    38 accessory gland, mated 4-day male    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    32 central nervous system, larvae L3    39 central nervous system, pupae P8    150 head, virgin 1-day female    105 head, virgin 4-day female    72 head, virgin 20-day female    57 head, mated 1-day female    67 head, mated 4-day female    60 head, mated 20-day female    42 head, mated 1-day male    157 head, mated 4-day male    119 head, mated 20-day male    102 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    39 digestive system, larvae L3 wandering    52 digestive system, 1-day adult    38 digestive system, 4-day adult    29 digestive system, 20-day adult    44 fat body, larvae L3 wandering    7 fat body, white prepupae    19 fat body, pupae P8    40 carcass, larvae L3 wandering    32 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    22 ovary, virgin 4-day female    24 ovary, mated 4-day female    21 testis, mated 4-day male    38 accessory gland, mated 4-day male    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    34 embryo 04-06hr    36 embryo 06-08hr    51 embryo 08-10hr    64 embryo 10-12hr    105 embryo 12-14hr    64 embryo 14-16hr    82 embryo 16-18hr    87 embryo 18-20hr    111 embryo 20-22hr    98 embryo 22-24hr    101 larva L1    98 larva L2    59 larva L3 12hr old    38 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    55 white prepupae new    56 white prepupae 12hr    56 white prepupae 24hr    61 pupae 2d postWPP    75 pupae 3d postWPP    84 pupae 4d postWPP    84 adult male 01day    64 adult male 05day    65 adult male 30day    65 adult female 01day    54 adult female 05day    40 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr  (34) embryo 04-06hr  (36) embryo 06-08hr  (51) embryo 08-10hr  (64) embryo 10-12hr  (105) embryo 12-14hr  (64) embryo 14-16hr  (82) embryo 16-18hr  (87) embryo 18-20hr  (111) embryo 20-22hr  (98) embryo 22-24hr  (101) larva L1  (98) larva L2  (59) larva L3 12hr old  (38) larva L3 puffstage 1-2  (33) larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (55) white prepupae new  (56) white prepupae 12hr  (56) white prepupae 24hr  (61) pupae 2d postWPP  (75) pupae 3d postWPP  (84) pupae 4d postWPP  (84) adult male 01day  (64) adult male 05day  (65) adult male 30day  (65) adult female 01day  (54) adult female 05day  (40) adult female 30day  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    34 embryo 04-06hr    36 embryo 06-08hr    51 embryo 08-10hr    64 embryo 10-12hr  105 embryo 12-14hr    64 embryo 14-16hr    82 embryo 16-18hr    87 embryo 18-20hr  (111) embryo 20-22hr    98 embryo 22-24hr  101 larva L1    98 larva L2    59 larva L3 12hr old    38 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    55 white prepupae new    56 white prepupae 12hr    56 white prepupae 24hr    61 pupae 2d postWPP    75 pupae 3d postWPP    84 pupae 4d postWPP    84 adult male 01day    64 adult male 05day    65 adult male 30day    65 adult female 01day    54 adult female 05day    40 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    34 embryo 04-06hr    36 embryo 06-08hr    51 embryo 08-10hr    64 embryo 10-12hr    105 embryo 12-14hr    64 embryo 14-16hr    82 embryo 16-18hr    87 embryo 18-20hr    111 embryo 20-22hr    98 embryo 22-24hr    101 larva L1    98 larva L2    59 larva L3 12hr old    38 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    55 white prepupae new    56 white prepupae 12hr    56 white prepupae 24hr    61 pupae 2d postWPP    75 pupae 3d postWPP    84 pupae 4d postWPP    84 adult male 01day    64 adult male 05day    65 adult male 30day    65 adult female 01day    54 adult female 05day    40 adult female 30day    41 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    34 embryo 04-06hr    36 embryo 06-08hr    51 embryo 08-10hr    64 embryo 10-12hr    105 embryo 12-14hr    64 embryo 14-16hr    82 embryo 16-18hr    87 embryo 18-20hr    111 embryo 20-22hr    98 embryo 22-24hr    101 larva L1    98 larva L2    59 larva L3 12hr old    38 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    55 white prepupae new    56 white prepupae 12hr    56 white prepupae 24hr    61 pupae 2d postWPP    75 pupae 3d postWPP    84 pupae 4d postWPP    84 adult male 01day    64 adult male 05day    65 adult male 30day    65 adult female 01day    54 adult female 05day    40 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr  (34) embryo 04-06hr  (36) embryo 06-08hr  (51) embryo 08-10hr  (64) embryo 10-12hr  (105) embryo 12-14hr  (64) embryo 14-16hr  (82) embryo 16-18hr  (87) embryo 18-20hr  (111) embryo 20-22hr  (98) embryo 22-24hr  (101) larva L1  (98) larva L2  (59) larva L3 12hr old  (38) larva L3 puffstage 1-2  (33) larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (55) white prepupae new  (56) white prepupae 12hr  (56) white prepupae 24hr  (61) pupae 2d postWPP  (75) pupae 3d postWPP  (84) pupae 4d postWPP  (84) adult male 01day  (64) adult male 05day  (65) adult male 30day  (65) adult female 01day  (54) adult female 05day  (40) adult female 30day  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    34 embryo 04-06hr    36 embryo 06-08hr    51 embryo 08-10hr    64 embryo 10-12hr  105 embryo 12-14hr    64 embryo 14-16hr    82 embryo 16-18hr    87 embryo 18-20hr  111 embryo 20-22hr    98 embryo 22-24hr  101 larva L1    98 larva L2    59 larva L3 12hr old    38 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    55 white prepupae new    56 white prepupae 12hr    56 white prepupae 24hr    61 pupae 2d postWPP    75 pupae 3d postWPP    84 pupae 4d postWPP    84 adult male 01day    64 adult male 05day    65 adult male 30day    65 adult female 01day    54 adult female 05day    40 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    34 embryo 04-06hr    36 embryo 06-08hr    51 embryo 08-10hr    64 embryo 10-12hr    105 embryo 12-14hr    64 embryo 14-16hr    82 embryo 16-18hr    87 embryo 18-20hr    111 embryo 20-22hr    98 embryo 22-24hr    101 larva L1    98 larva L2    59 larva L3 12hr old    38 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    55 white prepupae new    56 white prepupae 12hr    56 white prepupae 24hr    61 pupae 2d postWPP    75 pupae 3d postWPP    84 pupae 4d postWPP    84 adult male 01day    64 adult male 05day    65 adult male 30day    65 adult female 01day    54 adult female 05day    40 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    289 Schneider line 2 Sg4    115 embryonic 1182-4H    99 embryonic GM2    68 embryonic Kc167    70 embryonic S1    118 embryonic S3    61 leg disc CME L1    105 wing disc CME-W2    54 wing disc ML-DmD8    38 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    65 wing disc ML-DmD21    79 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    49 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    136 mixed discs ML-DmD4-c1    84 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    67 tumorous blood cells mbn2    144 ovary fGS/OSS    41 ovary OSC    18 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (289) Schneider line 2 Sg4  (115) embryonic 1182-4H  (99) embryonic GM2  (68) embryonic Kc167  (70) embryonic S1  (118) embryonic S3  (61) leg disc CME L1  (105) wing disc CME-W2  (54) wing disc ML-DmD8  (38) wing disc ML-DmD9  (87) wing disc ML-DmD16-c3  (65) wing disc ML-DmD21  (79) wing disc ML-DmD32  (40) haltere disc ML-DmD17-c3  (49) eye-antennal disc ML-DmD11  (72) antennal disc ML-DmD20-c5  (136) mixed discs ML-DmD4-c1  (84) CNS ML-DmBG1-c1  (45) CNS ML-DmBG2-c2  (67) tumorous blood cells mbn2  (144) ovary fGS/OSS  (41) ovary OSC    18 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (289) Schneider line 2 Sg4  (115) embryonic 1182-4H    99 embryonic GM2    68 embryonic Kc167    70 embryonic S1  (118) embryonic S3    61 leg disc CME L1  105 wing disc CME-W2    54 wing disc ML-DmD8    38 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    65 wing disc ML-DmD21    79 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    49 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5  (136) mixed discs ML-DmD4-c1    84 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    67 tumorous blood cells mbn2  (144) ovary fGS/OSS    41 ovary OSC    18 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    289 Schneider line 2 Sg4    115 embryonic 1182-4H    99 embryonic GM2    68 embryonic Kc167    70 embryonic S1    118 embryonic S3    61 leg disc CME L1    105 wing disc CME-W2    54 wing disc ML-DmD8    38 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    65 wing disc ML-DmD21    79 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    49 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    136 mixed discs ML-DmD4-c1    84 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    67 tumorous blood cells mbn2    144 ovary fGS/OSS    41 ovary OSC    18 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    289 Schneider line 2 Sg4    115 embryonic 1182-4H    99 embryonic GM2    68 embryonic Kc167    70 embryonic S1    118 embryonic S3    61 leg disc CME L1    105 wing disc CME-W2    54 wing disc ML-DmD8    38 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    65 wing disc ML-DmD21    79 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    49 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    136 mixed discs ML-DmD4-c1    84 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    67 tumorous blood cells mbn2    144 ovary fGS/OSS    41 ovary OSC    18 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (289) Schneider line 2 Sg4  (115) embryonic 1182-4H  (99) embryonic GM2  (68) embryonic Kc167  (70) embryonic S1  (118) embryonic S3  (61) leg disc CME L1  (105) wing disc CME-W2  (54) wing disc ML-DmD8  (38) wing disc ML-DmD9  (87) wing disc ML-DmD16-c3  (65) wing disc ML-DmD21  (79) wing disc ML-DmD32  (40) haltere disc ML-DmD17-c3  (49) eye-antennal disc ML-DmD11  (72) antennal disc ML-DmD20-c5  (136) mixed discs ML-DmD4-c1  (84) CNS ML-DmBG1-c1  (45) CNS ML-DmBG2-c2  (67) tumorous blood cells mbn2  (144) ovary fGS/OSS  (41) ovary OSC    18 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (289) Schneider line 2 Sg4  115 embryonic 1182-4H    99 embryonic GM2    68 embryonic Kc167    70 embryonic S1  118 embryonic S3    61 leg disc CME L1  105 wing disc CME-W2    54 wing disc ML-DmD8    38 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    65 wing disc ML-DmD21    79 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    49 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5  136 mixed discs ML-DmD4-c1    84 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    67 tumorous blood cells mbn2  (144) ovary fGS/OSS    41 ovary OSC    18 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    289 Schneider line 2 Sg4    115 embryonic 1182-4H    99 embryonic GM2    68 embryonic Kc167    70 embryonic S1    118 embryonic S3    61 leg disc CME L1    105 wing disc CME-W2    54 wing disc ML-DmD8    38 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    65 wing disc ML-DmD21    79 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    49 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    136 mixed discs ML-DmD4-c1    84 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    67 tumorous blood cells mbn2    144 ovary fGS/OSS    41 ovary OSC    18 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    29 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    32 Ethanol 10% 3 hrs, larvae L3    30 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    35 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (35) cold shock, 4-day adult  (29) heat shock, 4-day adult  (29) Cadmium 50 mM 6 hrs, larvae L3  (31) Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  (28) Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3  (41) Ethanol 5% 3 hrs, larvae L3  (32) Ethanol 10% 3 hrs, larvae L3  (30) Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (35) Paraquat 10 mM 48 hrs, 4-day adult  (43) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    29 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    32 Ethanol 10% 3 hrs, larvae L3    30 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    29 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    32 Ethanol 10% 3 hrs, larvae L3    30 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    35 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    29 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    32 Ethanol 10% 3 hrs, larvae L3    30 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (35) cold shock, 4-day adult  29 heat shock, 4-day adult  29 Cadmium 50 mM 6 hrs, larvae L3  31 Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  28 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3  (41) Ethanol 5% 3 hrs, larvae L3  32 Ethanol 10% 3 hrs, larvae L3  30 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (35) Paraquat 10 mM 48 hrs, 4-day adult  (43) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    29 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    32 Ethanol 10% 3 hrs, larvae L3    30 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    29 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    23 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    32 Ethanol 10% 3 hrs, larvae L3    30 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_29 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • SD04216 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004574 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG15811-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rop Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 RopGD1523 lethal | embryonic stage | recessive RopA3 RopA19 RopE3 RopG8 RopG27 lethal | heat sensitive RopF3 RopG11 RopG17 lethal | recessive Rop9-1 RopA3 RopA16 RopA20 RopA27 RopE3 RopF3 RopG8 RopG11 RopG16 RopG17 RopG27 Ropunspecified partially lethal - majority die | recessive | heat sensitive RopF3 RopG17 Other Phenotypes Allele neurophysiology defective | heat sensitive RopF3 RopG11 RopG17 neurophysiology defective | larval stage | heat sensitive Rophs.PS neurophysiology defective | larval stage | heat sensitive (with RopA19) RopG11 neurophysiology defective | larval stage | heat sensitive (with RopF3) RopG17 neurophysiology defective | larval stage | heat sensitive (with RopG11) RopA19 neurophysiology defective | larval stage | heat sensitive (with RopG17) RopF3 neurophysiology defective | larval stage | recessive RopG27 neurophysiology defective | larval stage | recessive | heat sensitive RopF3 RopG11 RopG17 wild-type Rop+t10.2 Phenotype manifest in Allele central nervous system RopA3 RopG27 egg | germline clone RopG27 embryonic/first instar larval cuticle RopA3 RopG27 oocyte | germline clone RopG27 tracheal branch primordium RopA3 RopG27
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of Rop Class Mutagen Stocks Known lesion RopG27 amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes RopA3 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes RopG4478 P-element activity 1 -- RopGS10103 1 Yes Rop9-1 0 -- RopA16 0 -- RopA19 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes RopA20 0 -- RopA27 0 -- RopE3 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- RopF3 ethyl methanesulfonate 0 Yes RopG11 ethyl methanesulfonate 0 Yes RopG16 0 -- RopG17 ethyl methanesulfonate 0 Yes RopG8 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Ropunspecified 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rop Class Mutagen Stocks Known lesion RopGD1523 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RopHM05140 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RopKK100853 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rop+t10.2 in vitro construct - genomic fragment 0 Yes Rophs.PS in vitro construct - regulatory fusion 0 Yes RopMtnA.PH in vitro construct - regulatory fusion 0 Yes RopNIG.15811R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ropstop in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)9-7K (Kulkarni et al., 1994) Df(3L)A466 (Kulkarni et al., 1994) Df(3L)BSC735 (Christensen et al., 2009.2.28) Df(3L)Exel6100 (Ryder, 2004.4.26) Df(3L)GN19 (Harrison et al., 1995) Df(3L)GN34 (Wu et al., 1998, Harrison et al., 1994, Harrison et al., 1995) Df(3L)nab1600 (Clements et al., 2003) Df(3L)TE1 (Kulkarni et al., 1994, Harrison et al., 1995) Df(3L)TE3 (Kulkarni et al., 1994)
Not disrupted in	Df(3L)C175 (Harrison et al., 1995) Df(3L)E14 (Kulkarni et al., 1994) Df(3L)HR298 (Harrison et al., 1995) T(Y;3)B887 (Kulkarni et al., 1994)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1523} NA P{KK100853} NA P{NIG.15811R} NA P{TRiP.HM05140} NA reporter construct P{lacZRop.P} No heat-shock construct P{hsrop} NA characterization construct P{R8} NA P{R8ΔRop} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}RopG4478 NA P{GSV6}RopGS10103 NA
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cytokinesis inferred from mutant phenotype (Echard et al., 2004) neurotransmitter secretion inferred from mutant phenotype (Littleton et al., 1995, Harrison et al., 1994) regulation of pole plasm oskar mRNA localization inferred from mutant phenotype (Ruden et al., 2000) response to light stimulus inferred from mutant phenotype (Harrison et al., 1994) secretion by cell inferred from direct assay (Harrison et al., 1994) synaptic transmission inferred from direct assay (Harrison et al., 1994)
Cellular Component
CV term Evidence References
cytosol inferred from direct assay (Harrison et al., 1994) extrinsic to membrane inferred from direct assay (Harrison et al., 1994) terminal bouton inferred from direct assay (Harrison et al., 1994)
Terms Based on Predictions or Assertions ( 13 terms )
Molecular Function
CV term Evidence References
SNARE binding inferred from sequence or structural similarity (Littleton, 2000) syntaxin binding non-traceable author statement (Lev et al., 1995)
Biological Process
CV term Evidence References
exocytosis non-traceable author statement (Broadie, 1995) traceable author statement (Toonen and Verhage, 2003) neurotransmitter secretion non-traceable author statement (Lev et al., 1995, Lloyd et al., 2000) traceable author statement (Ruden et al., 2000) synaptic transmission non-traceable author statement (Broadie, 1995) traceable author statement (Toonen and Verhage, 2003) synaptic vesicle priming non-traceable author statement (Lloyd et al., 2000) synaptic vesicle transport traceable author statement (Toonen and Verhage, 2003) vesicle docking involved in exocytosis inferred from electronic annotation with InterPro:IPR001619 (FlyBase Curators et al., 2004-) vesicle-mediated transport inferred from sequence or structural similarity (Littleton, 2000) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Littleton et al., 1999) cytosol traceable author statement (Toonen and Verhage, 2003) membrane traceable author statement (Toonen and Verhage, 2003) vesicle membrane non-traceable author statement (Lev et al., 1995)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Rop - 5-HT7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - Ada anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG10496 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG11679 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG15236 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG2469 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG31910 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG33514 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG4004 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG4338 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG4766 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG5190 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG5961 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG6073 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG7033 experimental knowledge based (Guruharsha et al., 2011) Rop - CG7048 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - CG9062 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - Chc experimental knowledge based (Guruharsha et al., 2011) Rop - Cyp9b2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - Ent2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - Esp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - Hph anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - Psa experimental knowledge based (Guruharsha et al., 2011) Rop - Rpt2 experimental knowledge based (Guruharsha et al., 2011) Rop - Rpt5 experimental knowledge based (Guruharsha et al., 2011) Rop - TfIIEα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - Vps25 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - atms anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - bor experimental knowledge based (Guruharsha et al., 2011) Rop - bru-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - bsh anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - ena experimental knowledge based (Guruharsha et al., 2011) Rop - mib2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rop - sqh experimental knowledge based (Guruharsha et al., 2011) Rop - stas anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rop allele Gene References RopG27 nab (Clements et al., 2003) unspecified bchs (Simonsen et al., 2007) pbl (Gregory et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63973 DroID - A comprehensive database of gene and protein interactions. • FBgn0004574 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38493
Orthologs
OrthoDB Orthologs (52) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VHKS0 Dipteran inclusive ortholog search EOG6T4D3N Insect inclusive ortholog search EOG67WMHS Arthropod inclusive ortholog search EOG63FFBZ Metazoa inclusive ortholog search EOG66QKKJ
Orthologs in Drosophila Species (EOG6VHKS0)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Rop     Drosophila simulans   Dsim\GD13290 Y   Drosophila sechellia   Dsec\GM14010 Y   Drosophila erecta   Dere\GG14217 Y   Drosophila yakuba   Dyak\GE20645 Y   Drosophila ananassae   Dana\GF20177 Y   Drosophila persimilis   Dper\GL15772     Drosophila willistoni   Dwil\GK25120 Y   Drosophila virilis   Dvir\GJ15999 Y   Drosophila mojavensis   Dmoj\GI21525 Y   Drosophila grimshawi   Dgri\GH12659
Orthologs in non-Drosophila Dipterans (EOG6T4D3N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007300   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007282   Anopheles gambiae Malaria mosquito  Agam\AGAP003023   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013548
Orthologs in non-Dipteran Insects (EOG67WMHS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49146   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009810   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH25256   Atta cephalotes Leafcutter ant  Acep\ACEP16154   Camponotus floridanus Florida carpenter ant  Cflo\CFLO21043   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13152   Linepithema humile Argentine ant  Lhum\LH13401   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25080   Solenopsis invicta Red fire ant  Sinv\SINV11129   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000496   Acyrthosiphon pisum Pea aphid  Apim\ACYPI060825   Pediculus humanus Human body louse  Phum\PHUM491150   Tribolium castaneum Red flour beetle  Tcas\Rop
Orthologs in non-Insect Arthropods (EOG63FFBZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Fam50b   Daphnia pulex Water flea  Dpux\JGI_V11_17683   Ixodes scapularis Deer tick  Isca\ISCW002599
Orthologs in non-Arthropod Metazoa (EOG66QKKJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F27D9.1   Strongylocentrotus purpuratus Purple sea urchin  Spur\sec1/munc18   Danio rerio Zebrafish  Drer\si:rp71-10d23.3   Danio rerio Zebrafish  Drer\stxbp3   Danio rerio Zebrafish  Drer\si:rp71-10d23.3   Danio rerio Zebrafish  Drer\stxbp1a   Danio rerio Zebrafish  Drer\stxbp2   Xenopus tropicalis Western clawed frog  Xtro\stxbp2   Xenopus tropicalis Western clawed frog  Xtro\stxbp1   Xenopus tropicalis Western clawed frog  Xtro\stxbp3   Gallus gallus Domestic chicken  Ggal\STXBP1   Gallus gallus Domestic chicken  Ggal\STXBP3   Mus musculus House mouse  Mmus\Stxbp3   Mus musculus House mouse  Mmus\Stxbp1   Mus musculus House mouse  Mmus\Stxbp2   Rattus norvegicus Norway rat  Rnor\Stxbp1   Rattus norvegicus Norway rat  Rnor\Stxbp3   Rattus norvegicus Norway rat  Rnor\Stxbp2   Homo sapiens Human  Hsap\STXBP3   Homo sapiens Human  Hsap\STXBP2   Homo sapiens Human  Hsap\ENSG00000136854
Human Orthologs (3)
Gene OMIM HGNC Hsap\STXBP3     Hsap\STXBP2     Hsap\ENSG00000136854
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13290 Drosophila sechellia Dsec\GM14010 Drosophila erecta Dere\GG14217 Drosophila yakuba Dyak\GE20645 Drosophila ananassae Dana\GF20177 Drosophila pseudoobscura pseudoobscura Dpse\GA29333 Drosophila willistoni Dwil\GK25120 Drosophila virilis Dvir\GJ15999 Drosophila mojavensis Dmoj\GI21525 Drosophila grimshawi Dgri\GH17352
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	28929 y1 v1; P{TRiP.HM05140}attP2 39670 bw1; RopA3 st1/TM6B, Tb+ 4381 bw1; RopG27 st1/TM6B, Tb+ 27158 y1 w*; P{EP}RopG4478/TM3, Sb1 Ser1
Kyoto	107715 bw1; RopG27 st1/TM6B, Tb+ 205192 y1 w67c23; P{GSV6}RopGS10103/TM3, Sb1 Ser1
VDRC	v106242 P{KK100853}VIE-260B v19696 w1118; P{GD1523}v19696/TM3
Genomic Clones ( 1 )
	BACR48M07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 146 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD04216
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03017 AT19879 AT28536 GH02038 GH02138 GH06886 GH19796 GH26952 GM18764 GM22492 LD09042 LP04756 LP07738 RE05482 RE07706 RE16814 RE18129 RE23863 RE28338 RE30476 RE36582 RE46210 RE46982 RE47721 RE52586 RE58385 RE64319 RE69186 RH14447 RH16888 RH17891 RH24186 RH25366 RH34254 RH34567 RH35282 RH36695 RH38842 RH39567 RH40026 RH40224 RH47481 RH47918 RH57513 RH59930 RH60281 RH63707 RH69329 RH71481 SD01578 SD05156 SD05552 SD08190 SD08340 SD11184 SD13336 SD14038 SD17577 SD17988 SD18923 SD18953 SD20189 SD20530 SD21234
Other clones	6984 11345 52697 74610 74812 75194 81437 101217 101452 121941 144847 156772 156796 161829 176214 177457 214253.18 214531.13 283290 522080 528245 571319 CK01.29B.D3 CK01.33A.C7 CK01.61A.H7 CK01.76A.A6 CK01.86B.E3 CK01.101C.H8 CK01.103C.C3 CK01.115C.D12 CK01.115D.A9 CK01.118A.G1 CK01.118A.G3 CK01.123A.E1 CK01.128C.G9 EK025914 EK028212 EK059435 EK063732 EK068118 EK079310 EK085746 EK086508 EK086910 EK089719 EK093717 EK099725 EK125321 EK128105 EK141031 EK142651 EK146164 EK151316 EK154003 EK168614 EK172560 EK189102 EK204511 EK206030 EK215112 EK228011 EK228249 EK243532 EK251041 EK259934 EK260060 EK266060 EK276638 EN01330 EN05806 EN06809 EN09320 EN14204 EN16619 EP10507 EP10614 EP10763 ESG012c.E21_C04 FGC005E07 IP12927 IP13127
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004574 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38493
Antibody Information
	monoclonal (Harrison et al., 1994) polyclonal (Salzberg et al., 1993)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells. (Echard et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Rop interacts with Syx1A in vivo, and is a rate-limiting regulator of exocytosis that has both positive and inhibitory functions in neurotransmission. (Wu et al., 1998) The Rop gene product plays a role in general secretion and is required for exocytosis from a variety of secretory tissues. (Broadie, 1995) Rop is a member of the "Sec1" family of proteins involved in synaptic transmission and general secretion. (Lev et al., 1995) The Rop protein plays a negative role in neurotransmitter release, in addition to its previously identified positive function possible by modulation of docking of synaptic vesicles or activation of a pre-fusion complex at the active zone. (Littleton et al., 1995) Loss of Rop blocks the extracellular accumulation of secreted products and temperature sensitive Rop mutations disrupt synaptic transmission in adults. Rop mutant clones fail to develop. (Harrison et al., 1994) Rop plays a positive role in synaptic transmission and functions in both secretion and neurotransmission. (Harrison et al., 1994) Ras64B/Rop bidirectional promoter supports the transcription of both genes. Mobility shift assays indicate the binding of a single major protein complex to the Ras64B/Rop promoter. (Lightfoot et al., 1994) A 3-5 fold induction of Rop protein in third instar larvae causes a dramatic decrease in neurotransmitter release. The number of spontaneous vesicle fusions is reduced by more than 50%, though calcium dependence of neurotransmitter release is not significantly altered. (Schulze et al., 1994) Rop plays a negative role in neurotransmitter release in vivo possibly by modulating the docking of synaptic vesicles or activation of a pre-fusion complex at the active zone. (Schulze et al., 1994) Rop is divergently transcribed from Ras64B, and the two transcription units are only 93 bases apart. Based on sequence similarities with related interacting proteins in yeast, it is suggested that Rop and Ras64B gene products are not simply coexpressed, but may interact with each other. (Salzberg et al., 1993) A bidirectional promoter regulates the Ras64B gene in one direction and the Rop gene in the other direction. (Cohen et al., 1988)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079192 NM_001274475 RefSeq (Proteins) • NP_523916 NP_001261404 DDBJ / EMBL / GenBank • AAB60242 AAF47844 AAK93518 AGB94099 AI532637 AY052094 CAA47658 CAA47659 U15967 X07255 X67218 X67219 Entrez Gene - A searchable database of RefSeq genes. • 38493 UniProtKB/Swiss-Prot • Q07327
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sec1-like protein (IPR001619) Sec1-like, domain 2 (IPR027482) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • SD04216
Linkouts
	BioGRID - A database of protein and genetic interactions • 63973 DroID - A comprehensive database of gene and protein interactions. • FBgn0004574 DRSC - Results from RNAi screens. • FBgn0004574 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004574 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG15811-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004574 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38493 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38493 modMine - Data generated by the modENCODE project. • FBgn0004574
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG15811 (Verstreken and Bellen, 2000.9.4, Dorer et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009) cs1 (Cohen et al., 1988, ) l(3)64Ah (Clements et al., 2003, Harrison et al., 1995, ) l(3)SH16 (Harrison et al., 1995) ras2 (Lev, 1988.3.28) Rop (Pulipparacharuvil et al., 2005, Gregory et al., 2007, Harrison, 1994.10.14, Cha et al., 2008, Harrison et al., 1994, Romero et al., 2008, Christensen et al., 2009.2.28, Christensen et al., 2009.2.28, Dworkin et al., 2009, Matos et al., 2000, Japanese National Institute of Genetics, 2012.5.21, Munoz-Soriano et al., 2012) ROP (Adolfsen et al., 2004, Fabian-Fine et al., 2003, Verstreken et al., 2003, Toonen and Verhage, 2003, Yoshihara and Littleton, 2002, Lloyd et al., 2000, Wu et al., 1999) rop (Clements et al., 2003, Ruden et al., 1999, Wu et al., 1998, Wu and Bellen, 1997, Wu et al., 1997, Wu et al., 1997, Linial and Parnas, 1996, Parfitt et al., 1995, Bate and Broadie, 1995, Lev et al., 1995, Schulze et al., 1994, Lightfoot et al., 1994) rop(Sec1) (Echard et al., 2004) SEC1 (Dorer et al., 2006)
Name Synonym	Ras opposite (Romero et al., 2008) ras opposite (Simonsen et al., 2007)
Secondary FlyBase IDs
	FBgn0014289
References ( 86 )
Generate a list of	All references relating to this gene ( 86 )
List References by type	Research paper  ( 35 ) Supplementary material  ( 7 ) Review  ( 10 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 13 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Letter  ( 1 )
Recent research papers ( 3 )
	Munoz-Soriano et al., 2012, J. Mol. Neurosci. 48(1): 136--143 Septin 4, the Drosophila Ortholog of Human CDCrel-1, Accumulates in parkin Mutant Brains and is Functionally Related to the Nedd4 E3 Ubiquitin Ligase. [FBrf0219099] Palgi et al., 2012, BMC Genomics 13: 134 Gene expression analysis of Drosophilaa Manf mutants reveals perturbations in membrane traffic and major metabolic changes. [FBrf0218515] Koppen et al., 2011, Proteomics 11(22): 4397--4410 Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. [FBrf0216610] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...