Gene Dmel\zfh1

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\zfh1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UAG) postulated; FBrf0216884.

gene_with_stop_codon_read_through ; SO:0000697

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

zfh1-RA

FBtr0085702

NM_170523

3560

747

zfh1-RB

FBtr0085701

NM_057502

5803

1054

zfh1-RE

FBtr0331180

NM_170522

5803

1206

Additional Transcript Data & Comments

Reported size (kB)

7.5 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

zfh1-PA

FBpp0085064

83.8

747

7.54

NP_733402

AAF57084

zfh1-PB

FBpp0085063

116.6

1054

7.15

NP_476850

AAF57083

zfh1-PE

FBpp0303607

133.0

1206

7.52

NP_733401

AAN14258

Additional Polypeptide Data & Comments

Reported size (kDa)

1060 (aa); 145 (kD observed); 117 (kD predicted)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Homeobox, conserved site (IPR017970)

Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087)

Zinc finger, C2H2-like (IPR015880)

Homeodomain (IPR001356)

Zinc finger, C2H2 (IPR007087)

Homeodomain-like (IPR009057)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P28166

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

central nervous system | restricted

mesoderm

cardioblast

adult muscle precursor cell

pole cell

embryonic/larval visceral muscle

embryonic/larval somatic muscle

gonad

embryonic/larval dorsal vessel

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

head mesoderm anlage

Comment:anlage in statu nascendi

mesoderm anlage

Comment:anlage in statu nascendi

trunk mesoderm anlage

embryonic stage 7 -- 8

anterior endoderm anlage

head mesoderm

trunk mesoderm

ventral ectoderm

embryonic stage 9 -- 10

head mesoderm

trunk mesoderm

ventral nerve cord primordium

embryonic stage 9 -- 11

visceral mesoderm | restricted

(Kusch and Reuter, 1999)

embryonic stage 11 -- 12

cardiogenic mesoderm

embryonic neuroblast of ventral nerve cord primordium

fat body/gonad primordium

lateral cord glial cell

lateral cord neuron

midline ventral glial cell

pericardial cell primordium

Comment:reported as pericardial cell specific anlage

plasmatocyte primordium

Comment:reported as plasmatocytes anlage

trunk mesoderm

embryonic stage 13 -- 16

embryonic brain

embryonic central brain glial cell

embryonic central brain neuron

embryonic leading edge cell

embryonic/larval fat body

embryonic/larval lymph gland

embryonic/larval pericardial cell

lateral cord glial cell

lateral cord neuron

ventral nerve cord

Additional Descriptive Data

The zfh1 transcript distribution pattern corresponds closely to that of zfh1 protein.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

visceral muscle

mesoderm

adult muscle precursor cell

cardioblast

(Lai et al., 1991, Bodmer, 1993)

central nervous system | restricted

embryonic/larval dorsal vessel

(Lai et al., 1991, Bodmer, 1993)

VUM neuron

ventral intersegmental neuron

pole cell

gonad

lateral ipsisegmental neuron

pericardial cell

RP neuron

aCC neuron

somatic muscle

embryonic pericardial cell

• nucleus

(Su et al., 1999)

Comment:expressed in non-eve-expressing pericardial cells

embryonic stage 12 -- 15

RP2 motor neuron

(Bhat and Apsel, 2004)

embryonic stage 15 -- 17

GW neuron

(Lundell et al., 2003)

Additional Descriptive Data

zfh1 protein is abundant in staged extracts from embryos between 3 and 18 hours. zfh1 protein is first detected in the presumptive procephalic mesoderm and then in the ventral mesoderm and pole cells. In late stage 8, strong staining is seen in a ~30 cell-wide band of ventral mesoderm. In stage 11, zfh1 protein is detected mainly in ectodermally-derived CNS structures and is thought to be present in most identifiable motor neurons including the aCC, RP, VUM, ventral intersegmental, and lateral ipsisegmental neurons. In stage 13, zfh1 protein is abundant in the CNS as well as in some mesodermally derived structures, including the dorsal vessel, gonadal support cells, and laterally located adult muscle precursor cells of the thoracic and abdominal segments. At stage 15, two rows of dorsal vessel cardioblasts are strongly stained as dorsal closure is completed. The CNS has condensed and shows staining of segmentally repeated subsets of neurons. Weak staining persists in the somatic and visceral musculature.

Markers that uniquely identify the cells of the NB3-7 lineage were used to examine the serotonin expressing cell lineages.

(Lundell et al., 2003)

zfh1 protein is initially expressed in a subset of heart precursor cells. Later expression is observed in all cells that form the dorsal vessel, cardial cells as well as pericardial cells.

(Bodmer, 1993)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

nucleus

inferred from direct assay

(Meister and Braun, 1995.10)

Expression Deduced from Reporters

Reporter: P{PZ}zfh1⁰⁰⁸⁶⁵

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval brain

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		23
central nervous system, pupae P8		9
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		3
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		6
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		6
fat body, white prepupae		21
fat body, pupae P8		13
carcass, larvae L3 wandering		8
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		4
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		3
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		27
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		(27)
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		27
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		27
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		27
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		27
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		27
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		10
embryo 02-04hr		8
embryo 04-06hr		23
embryo 06-08hr		24
embryo 08-10hr		27
embryo 10-12hr		19
embryo 12-14hr		26
embryo 14-16hr		26
embryo 16-18hr		19
embryo 18-20hr		17
embryo 20-22hr		11
embryo 22-24hr		10
larva L1		8
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		9
white prepupae new		11
white prepupae 12hr		15
white prepupae 24hr		12
pupae 2d postWPP		7
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		5
adult male 30day		4
adult female 01day		6
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		64
Schneider line 2 Sg4		25
embryonic 1182-4H		48
embryonic GM2		49
embryonic Kc167		36
embryonic S1		54
embryonic S3		46
leg disc CME L1		38
wing disc CME-W2		30
wing disc ML-DmD8		39
wing disc ML-DmD9		32
wing disc ML-DmD16-c3		76
wing disc ML-DmD21		7
wing disc ML-DmD32		63
haltere disc ML-DmD17-c3		74
eye-antennal disc ML-DmD11		51
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		64
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		68
ovary fGS/OSS		53
ovary OSC		110
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(64)
Schneider line 2 Sg4		25
embryonic 1182-4H		(48)
embryonic GM2		(49)
embryonic Kc167		(36)
embryonic S1		(54)
embryonic S3		(46)
leg disc CME L1		(38)
wing disc CME-W2		(30)
wing disc ML-DmD8		(39)
wing disc ML-DmD9		(32)
wing disc ML-DmD16-c3		(76)
wing disc ML-DmD21		7
wing disc ML-DmD32		(63)
haltere disc ML-DmD17-c3		(74)
eye-antennal disc ML-DmD11		(51)
antennal disc ML-DmD20-c5		(28)
mixed discs ML-DmD4-c1		(28)
CNS ML-DmBG1-c1		(64)
CNS ML-DmBG2-c2		(38)
tumorous blood cells mbn2		(68)
ovary fGS/OSS		(53)
ovary OSC		(110)
ovary OSS		(33)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		64
Schneider line 2 Sg4		25
embryonic 1182-4H		48
embryonic GM2		49
embryonic Kc167		36
embryonic S1		54
embryonic S3		46
leg disc CME L1		38
wing disc CME-W2		30
wing disc ML-DmD8		39
wing disc ML-DmD9		32
wing disc ML-DmD16-c3		76
wing disc ML-DmD21		7
wing disc ML-DmD32		63
haltere disc ML-DmD17-c3		74
eye-antennal disc ML-DmD11		51
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		64
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		68
ovary fGS/OSS		53
ovary OSC		(110)
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		64
Schneider line 2 Sg4		25
embryonic 1182-4H		48
embryonic GM2		49
embryonic Kc167		36
embryonic S1		54
embryonic S3		46
leg disc CME L1		38
wing disc CME-W2		30
wing disc ML-DmD8		39
wing disc ML-DmD9		32
wing disc ML-DmD16-c3		76
wing disc ML-DmD21		7
wing disc ML-DmD32		63
haltere disc ML-DmD17-c3		74
eye-antennal disc ML-DmD11		51
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		64
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		68
ovary fGS/OSS		53
ovary OSC		110
ovary OSS		33
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		64
Schneider line 2 Sg4		25
embryonic 1182-4H		48
embryonic GM2		49
embryonic Kc167		36
embryonic S1		54
embryonic S3		46
leg disc CME L1		38
wing disc CME-W2		30
wing disc ML-DmD8		39
wing disc ML-DmD9		32
wing disc ML-DmD16-c3		76
wing disc ML-DmD21		7
wing disc ML-DmD32		63
haltere disc ML-DmD17-c3		74
eye-antennal disc ML-DmD11		51
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		64
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		68
ovary fGS/OSS		53
ovary OSC		110
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(64)
Schneider line 2 Sg4		25
embryonic 1182-4H		(48)
embryonic GM2		(49)
embryonic Kc167		(36)
embryonic S1		(54)
embryonic S3		(46)
leg disc CME L1		(38)
wing disc CME-W2		30
wing disc ML-DmD8		(39)
wing disc ML-DmD9		32
wing disc ML-DmD16-c3		(76)
wing disc ML-DmD21		7
wing disc ML-DmD32		(63)
haltere disc ML-DmD17-c3		(74)
eye-antennal disc ML-DmD11		(51)
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		(64)
CNS ML-DmBG2-c2		(38)
tumorous blood cells mbn2		(68)
ovary fGS/OSS		(53)
ovary OSC		(110)
ovary OSS		(33)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		64
Schneider line 2 Sg4		25
embryonic 1182-4H		48
embryonic GM2		49
embryonic Kc167		36
embryonic S1		54
embryonic S3		46
leg disc CME L1		38
wing disc CME-W2		30
wing disc ML-DmD8		39
wing disc ML-DmD9		32
wing disc ML-DmD16-c3		76
wing disc ML-DmD21		7
wing disc ML-DmD32		63
haltere disc ML-DmD17-c3		74
eye-antennal disc ML-DmD11		51
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		64
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		68
ovary fGS/OSS		53
ovary OSC		110
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		64
Schneider line 2 Sg4		25
embryonic 1182-4H		48
embryonic GM2		49
embryonic Kc167		36
embryonic S1		54
embryonic S3		46
leg disc CME L1		38
wing disc CME-W2		30
wing disc ML-DmD8		39
wing disc ML-DmD9		32
wing disc ML-DmD16-c3		76
wing disc ML-DmD21		7
wing disc ML-DmD32		63
haltere disc ML-DmD17-c3		74
eye-antennal disc ML-DmD11		51
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		64
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		68
ovary fGS/OSS		53
ovary OSC		110
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		13
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		12
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		7
Paraquat 10 mM 48 hrs, 4-day adult		8
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Show all FlyExpress processed images for zfh1

BDGP expression data

•

SD06902

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004606

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG1322-RB

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^A3.eya

zfh1^Scer\UAS.cPa

lethal | embryonic stage | recessive

lethal | recessive

zfh1⁰⁰⁸⁶⁵

viable, with Scer\GAL4^A3.eya, Scer\GAL80^ts.αTub84B

zfh1^Scer\UAS.cPa

viable, with Scer\GAL4^pnr-MD237

zfh1^GD1565

Other Phenotypes

Allele

neuroanatomy defective

neuroanatomy defective, with Scer\GAL4^eg-Mz360

zfh1^Scer\UAS.cLa

neuroanatomy defective, with Scer\GAL4^elav-C155

zfh1^Scer\UAS.cLa

neuroanatomy defective, with Scer\GAL4^s.gcm

zfh1^Scer\UAS.cLa

neuroanatomy defective (with zfh1⁵)

zfh1^75.26

neuroanatomy defective (with zfh1^75.26)

zfh1⁵

visible | heat sensitive

zfh1^hs.PL

wild-type

zfh1^hs.PL

Phenotype manifest in

Allele

A1-7 dorsal acute muscle 1, with Scer\GAL4^how-24B

zfh1^Scer\UAS.cPa

A1-7 dorsal oblique muscle 1, with Scer\GAL4^how-24B

zfh1^Scer\UAS.cPa

abdominal posterior fascicle & axon, with Scer\GAL4^elav-C155

zfh1^Scer\UAS.cLa

abdominal segmental nerve

abdominal segmental nerve (with zfh1⁵)

zfh1^75.26

abdominal segmental nerve (with zfh1^75.26)

zfh1⁵

cardioblast

zfh1²

cyst progenitor cell | somatic clone

dMP2 neuron & axon, with Scer\GAL4^22B

zfh1^Scer\UAS.cLa

embryonic/larval dorsal trunk

embryonic/larval dorsal vessel

zfh1²

embryonic/larval lymph gland

zfh1¹

embryonic/larval lymph gland | embryonic stage

zfh1²

embryonic/larval pericardial cell

zfh1²

embryonic/larval somatic muscle

zfh1²

embryonic somatic muscle (with Df(3R)GC14)

zfh1²

EW1 neuron & axon, with Scer\GAL4^eg-Mz360

zfh1^Scer\UAS.cLa

EW2 neuron & axon, with Scer\GAL4^eg-Mz360

zfh1^Scer\UAS.cLa

EW3 neuron & axon, with Scer\GAL4^eg-Mz360

zfh1^Scer\UAS.cLa

eye | heat sensitive

zfh1^hs.PL

fat body/gonad primordium

garland cell

zfh1¹

germline cell

gonad | precursor

zfh1^hs.PL

gonadal sheath proper primordium

zfh1^unspecified

gonadal sheath proper primordium | male (with Df(3R)GC14)

zfh1^75.26

heart primordium

hemocyte | embryonic stage

zfh1²

intersegmental nerve

intersegmental nerve (with zfh1⁵)

zfh1^75.26

intersegmental nerve (with zfh1^75.26)

zfh1⁵

macrochaeta & thorax | conditional ts

zfh1^hs.PL

male germline cell | supernumerary, with Scer\GAL4^A3.eya, Scer\GAL80^ts.αTub84B

zfh1^Scer\UAS.cPa

motor nerve & axon, with Scer\GAL4^s.gcm

zfh1^Scer\UAS.cLa

pericardial cell

zfh1²

presumptive embryonic/larval central nervous system | heat sensitive

zfh1^hs.PL

somatic cell of testis | supernumerary, with Scer\GAL4^A3.eya, Scer\GAL80^ts.αTub84B

zfh1^Scer\UAS.cPa

testis, with Scer\GAL4^A3.eya, Scer\GAL80^ts.αTub84B

zfh1^Scer\UAS.cPa

Classical Alleles ( 23 )

For All Classical Alleles Show

Allele of zfh1	Class	Mutagen	Stocks	Known lesion
zfh1⁰⁰⁸⁶⁵		P-element activity	1	Yes
zfh1^CB-0034-3			1	--
zfh1^MB07519			1	--
zfh1^NP5453			1	--
zfh1²	loss of function allele, amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
zfh1^65.34	amorphic allele - molecular evidence, amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
zfh1^75.26	amorphic allele - molecular evidence, amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
zfh1¹⁰	loss of function allele	ethyl methanesulfonate	0	--
zfh1¹¹	loss of function allele	ethyl methanesulfonate	0	--
zfh1¹²	loss of function allele	ethyl methanesulfonate	0	--
zfh1¹³	loss of function allele	ethyl methanesulfonate	0	--
zfh1¹⁴	loss of function allele	ethyl methanesulfonate	0	--
zfh1¹⁵	loss of function allele	ethyl methanesulfonate	0	--
zfh1¹⁶	loss of function allele	ethyl methanesulfonate	0	--
zfh1¹	loss of function allele	ethyl methanesulfonate	0	--
zfh1³	loss of function allele	ethyl methanesulfonate	0	--
zfh1⁴	loss of function allele	ethyl methanesulfonate	0	--
zfh1⁵	loss of function allele	ethyl methanesulfonate	0	--
zfh1⁶	loss of function allele	ethyl methanesulfonate	0	--
zfh1⁷	loss of function allele	ethyl methanesulfonate	0	--
zfh1⁸	loss of function allele	ethyl methanesulfonate	0	--
zfh1⁹	loss of function allele	ethyl methanesulfonate	0	--
zfh1^unspecified		ethyl methanesulfonate	0	--

Alleles Carried on Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Allele of zfh1	Mutagen	Stocks	Known lesion
zfh1^GD1565	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
zfh1^{CIDm.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - amino acid replacement	1	Yes
zfh1^GL01066	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
zfh1^GL01539	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
zfh1^JF02509	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
zfh1^KK109931	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
zfh1^Scer\UAS.cPa	in vitro construct - regulatory fusion	1	Yes
zfh1^{ΔHD.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - deletion	1	Yes
zfh1^Act5C.PP	in vitro construct - regulatory fusion	0	Yes
zfh1^dsRNA.cKa	in vitro construct - RNAi	0	Yes
zfh1^dsRNA.si.cKa	in vitro construct - RNAi	0	Yes
zfh1^hs.PL	in vitro construct - regulatory fusion in vitro construct - minigene	0	Yes
zfh1^NIG.1322R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
zfh1^Scer\UAS.cLa	in vitro construct - regulatory fusion	0	Yes
zfh1^Scer\UAS.cLb	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)20

(Broihier et al., 1998, Lai et al., 1993)

Df(3R)A177.X1

Df(3R)A177.X3

Df(3R)A177der20

Df(3R)A177der25

Df(3R)A177der26

Df(3R)Exel7378

(Ryder, 2004.4.26)

Df(3R)Exel8194

(Ryder, 2004.4.26)

Df(3R)Exel9020

(Ryder, 2004.4.26)

Df(3R)P75A.A1

(Justice et al., 1995)

Df(3R)PH3

(BDGP Project Members, 1994-1999)

Df(3R)tll-pgx

(Lai et al., 1991, Lai et al., 1993)

T(Y;3)R133

Not disrupted in

Df(3R)A113

Df(3R)A177der21

Df(3R)A177der22

Df(3R)PGX8

(Spradling et al., 1999, Lai et al., 1993, BDGP Project Members, 1994-1999, Justice et al., 1995)

Df(3R)tll-e

Not duplicated in

Ts(YLt;3Rt)A113

(Justice et al., 1995)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

heat-shock construct

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{PZ}zfh1⁰⁰⁸⁶⁵

miscellaneous insertions

P{GMroX1}99F

P{RS3}zfh1^CB-0034-3

insertion of enhancer trap binary system

Mi{ET1}zfh1^MB07519

P{GawB}zfh1^NP5453

Gene Ontology: Function, Process & Cellular Component ( 20 unique terms )

Terms Based on Experimental Evidence ( 13 terms )

Molecular Function

CV term

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

References

inferred from direct assay

(Postigo et al., 1999)

RNA polymerase II regulatory region sequence-specific DNA binding

inferred from direct assay

(Postigo et al., 1999)

Biological Process

CV term

antimicrobial humoral response

References

inferred from mutant phenotype

(Kleino et al., 2005)

garland nephrocyte differentiation

inferred from mutant phenotype

(Sellin et al., 2006)

germ cell migration

inferred from mutant phenotype

hemocyte development

inferred from mutant phenotype

(Frandsen et al., 2008)

lymph gland development

inferred from mutant phenotype

(Sellin et al., 2006)

mesoderm development

inferred from expression pattern AND inferred from genetic interaction with sna AND inferred from genetic interaction with twi

motor neuron axon guidance

inferred from mutant phenotype

(Layden et al., 2006)

negative regulation of transcription from RNA polymerase II promoter

inferred from direct assay

(Postigo et al., 1999)

nervous system development

inferred from expression pattern

somatic stem cell division

inferred from mutant phenotype

(Leatherman and DiNardo, 2008)

Cellular Component

CV term

References

nucleus

inferred from direct assay

Terms Based on Predictions or Assertions ( 10 terms )

Molecular Function

CV term

References

DNA binding

traceable author statement

(FlyBase Curators et al., 2004-)

zinc ion binding

inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880

Biological Process

CV term

central nervous system development

References

non-traceable author statement

germ cell migration

traceable author statement

(Wylie, 1999)

gonad development

traceable author statement

(Howard, 1998)

heart development

non-traceable author statement

(Cripps and Olson, 2002)

mesoderm development

non-traceable author statement

muscle organ development

non-traceable author statement

pole cell migration

traceable author statement

(Howard, 1998)

Cellular Component

CV term

References

nucleus

inferred from sequence or structural similarity with EMBL:L48363

(FlyBase, 1992-)

non-traceable author statement

gene_with_stop_codon_read_through

Sequence Ontology: Class of Gene

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

zfh1 allele

Gene

References

(Franch-Marro and Casanova, 2000)

zfh1^75.26

tin

(Franch-Marro and Casanova, 2000)

External Data

y¹ sc^* v¹; P{TRiP.GL01066}attP2

BioGRID - A database of protein and genetic interactions

•

68501

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0004606

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

43650

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

P28166

Orthologs

OrthoDB Orthologs (33) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6C88BS

Dipteran inclusive ortholog search

EOG6HMJJB

Insect inclusive ortholog search

EOG6XSJKR

Arthropod inclusive ortholog search

EOG6DJHC9

Metazoa inclusive ortholog search

EOG657J1N

Orthologs in Drosophila Species (EOG6C88BS)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12131

Drosophila persimilis

Dper\GL13515

Drosophila willistoni

Dwil\GK14154

Drosophila virilis

Dvir\GJ24242

Drosophila mojavensis

Dmoj\GI22121

Drosophila grimshawi

Dgri\GH14214

Orthologs in non-Drosophila Dipterans (EOG6HMJJB)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002451

Anopheles gambiae

Malaria mosquito

Agam\AGAP000779

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ012784

Orthologs in non-Dipteran Insects (EOG6XSJKR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB49657

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010485

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH21605

Atta cephalotes

Leafcutter ant

Acep\ACEP16197

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO26878

Linepithema humile

Argentine ant

Lhum\LH19365

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB25049

Solenopsis invicta

Red fire ant

Sinv\SINV25541

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008075

Bombyx mori

Silkmoth

Bmor\BGIBMGA002551

Pediculus humanus

Human body louse

Phum\PHUM017930

Tribolium castaneum

Red flour beetle

Tcas\TC011114

Orthologs in non-Insect Arthropods (EOG6DJHC9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\rbcL

Ixodes scapularis

Deer tick

Isca\ISCW015893

Orthologs in non-Arthropod Metazoa (EOG657J1N)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\zag-1

Danio rerio

Zebrafish

Drer\si:ch211-159p3.1

Mus musculus

House mouse

Mmus\Zeb1

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12131

Drosophila persimilis

Dper\GL13515

Drosophila willistoni

Dwil\GK14154

Drosophila virilis

Dvir\GJ24242

Drosophila mojavensis

Dmoj\GI22121

Drosophila grimshawi

Dgri\GH14214

Stocks & Reagents

Stocks Listed in FlyBase ( 13 )

Bloomington

6879

w¹¹¹⁸; P{UAS-zfh1.P}2B

6881

w¹¹¹⁸; P{UAS-zfh1-ΔHD}2B

6880

w¹¹¹⁸; P{UAS-zfh1-CIDm}2B

25351

w¹¹¹⁸; Mi{ET1}zfh1^MB07519

38929

43195

y¹ sc^* v¹; P{TRiP.GL01539}attP40

29347

y¹ v¹; P{TRiP.JF02509}attP2/TM3, Sb¹

Kyoto

123013

w¹¹¹⁸; P{RS3}zfh1^CB-0034-3

105019

y^* w^*; P{GawB}zfh1^NP5453 / TM6, P{UAS-lacZ.UW23-1}UW23-1

VDRC

v103205

P{KK109931}VIE-260B

v42856

w¹¹¹⁸; P{GD1565}v42856

v42857

w¹¹¹⁸; P{GD1565}v42857

More stocks available...

Genomic Clones ( 1 )

BACR48I02

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 111 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0004606

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

43650

Antibody Information

monoclonal antibody

(Bhat and Apsel, 2004)

polyclonal

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: zfh1 CG1322

(Yao, 1999.11)

Source for database merge of

Additional comments

Other Comments

DNA-protein interactions: genome-wide binding profile assayed for zfh1 protein in 0-12 hr embryos; see mE1_TFBS_zfh1 collection report.

(Negre et al., 2011)

zfh1 is required for ventral motor axon exit from the CNS.

(Layden et al., 2006)

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.

(Kleino et al., 2005)

byn, supported by fkh, mediates the early specification of the caudal visceral mesoderm along with zfh1.

(Kusch and Reuter, 1999)

zfh1 is specifically required for the formation of the eve-expressing subset of pericardial cells (EPCs), without affecting the formation of their siblings, the founders of a dorsal body wall muscle (DA1). zfh1 specifies EPC development independently of numb.

(Su et al., 1999)

Loss of zfh1 activity disrupts the development of two distinct mesodermal populations: the caudal visceral mesoderm and the gonadal mesoderm. Ectopic expression of zfh1 is sufficient to induce additional gonadal mesodermal cells and to alter the temporal course of gene expression within these cells.

(Broihier et al., 1998)

tin is not required for early zfh1 expression throughout the mesoderm or for the refinement of this expression to lateral mesodermal clusters during stage 10. tin activity is required for aspects of zfh1 expression beginning at stage 11.

(Broihier et al., 1998)

zfh1 function is required for the transition of germ cells from the endoderm to the mesoderm, suggesting zfh1 is required for interactions between germ cells and somatic gonadal precursors (SGPs). Maternal zfh1 is not required for proper germ cell migration. zfh1 function is also required for aspects of caudal visceral mesoderm-specific gene expression and for the migration of these cells. tin and zfh1 cooperate in the specification of two tissues derived from the lateral mesoderm: the gonadal mesoderm and the fat body. zfh1 is a primary regulator of gonadal mesoderm, as it is both able to promote ectopic gonadal mesoderm formation and to alter the temporal course of gene expression in these cells.

(Broihier et al., 1998)

Mutants are isolated in an EMS mutagenesis screen to identify zygotic mutations affecting germ cell migration at discrete points during embryogenesis: mutants exhibit germ cell migration defects.

zfh1 is required for the development of both fat body and gonadal mesoderm in the embryo.

(Kalionis and O'Farrell, 1993)

Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.

(Braun et al., 1997)

One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus Engrailed binding site.

Phenotypic analysis reveals that the gene is not required for the initial segregation of the mesoderm or differentiation of mesodermally derived tissues, but loss of zfh1 function results in varying degrees of local errors in cell fate or positioning.

(Lai et al., 1993)

The product of zfh1 possesses one homeodomain and nine C₂H₂ zinc fingers. The novel arrangement of interspersed homeodomain and zinc-finger motifs in the primary sequences of the zfh1 and zfh2 gene products may signify an unusual mechanism of transcriptional regulation by these products.

The expression pattern of zfh1 suggests that the protein may be involved in neurogenesis and may have additional functions in mesoderm development.

Mesodermal expression of zfh1 is largely dependent upon twi and sna.