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Gene Dmel\zfh2

General Information
Symbol	Dmel\zfh2	Species	D. melanogaster
Name	Zn finger homeodomain 2	Annotation symbol	CG1449
Feature type	protein_coding_gene	FlyBase ID	FBgn0004607
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	Zfh-2
Genomic Location
Chromosome (arm)	4	Recombination map
Cytogenetic map	102C3-102C4	Sequence location	4:522,436..560,418 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Zn finger homeodomain 2 is referred to in FlyBase by the symbol Dmel\zfh2 (CG1449, FBgn0004607). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II regulatory region sequence-specific DNA binding. There is experimental evidence that it is involved in the biological process: nervous system development; imaginal disc-derived wing morphogenesis. 13 alleles are reported. The phenotypes of these alleles are annotated with: wing disc; sclerite; medial costal vein; alula; wing; wing vein L1; wing hinge. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Homeobox, conserved site; Homeodomain; Homeodomain-like; Zinc finger C2H2-type/integrase DNA-binding domain; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1627320_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of zfh2. Gene sequence location is 4:522436..560418.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 102C3-102C4
Experimentally Determined Cytological Location
Cytogenetic map Notes References 102C1-102C2   (determined by in situ hybridisation)   (Fortini et al., 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\zfh2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) zfh2-RA FBtr0089070  NM_079884   10619   3005 zfh2-RB FBtr0307167  NM_001258496   10613   3003 zfh2-RC FBtr0332913  NM_001272130   10141   2805
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein zfh2-PA   FBpp0088139   332.0   3005   6.57     NP_524623   AAF59339 zfh2-PB   FBpp0297996   331.8   3003   6.59     NP_001245425   AFH06785 zfh2-PC   FBpp0305133   309.5   2805   6.56     NP_001259059   AGB96549
Additional Polypeptide Data & Comments
Reported size (kDa)	3005 (aa); 332 (kD predicted) (Fortini et al., 1991) 3005 (aa) (Lundell and Hirsh, 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Homeobox, conserved site (IPR017970) Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087) Zinc finger, C2H2-like (IPR015880) Homeodomain (IPR001356) Zinc finger, C2H2 (IPR007087) Homeodomain-like (IPR009057)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014135 AAF59339   AC009535   AC010668   AC018478   AF242879   AF242880   AE014135 AFH06785   AE014135 AGB96549   AI134433   AI406135   AI533151   AI542796   AW940433   AY060681 AAL28229   AY312816 AAQ67630   AY312817 AAQ67631   AY312818 AAQ67632   AY312819 AAQ67633   AY312820 AAQ67634   AY312821 AAQ67635   AY312822 AAQ67636   AY312823 AAQ67637   AY312824 AAQ67638   AY312825 AAQ67639   AY312826 AAQ67640   AY312827 AAQ67641   AY312828 AAQ67642   AY312829 AAQ67643   AY312830 AAQ67644   AY312831 AAQ67645   AY312832 AAQ67646   AY312833 AAQ67647   AY312834 AAQ67648   AY312835 AAQ67649   AY312836 AAQ67650   AY312837 AAQ67651   AY312838 AAQ67652   AY312839 AAQ67653   BG639715   BT088973   CO178406   CO191104   CO263845   CO267728   CO270367   CO270684   CO280192   CO284497   CO294448   CO295904   CO295935   CO299791   CO299813   CO304677   CO304988   CO306572   CO308213   CO308501   CO311196   CO314100   CO317384   CO317843   CO318193   CO319597   CO320802   CO323063   CO323223   CO327612   CO328835   CO333507   CO340691   CO340734   CO341035   CO341416   CO344839   EC051878   EC053951   EC054366   EC056074   EC057514   EC061800   EC064101   EC065778   EC068656   EC071314   EC073183   EC074655   EC075372   EC075640   EC076161   EC083727   EC087127   EC089604   EC195819   EC197314   EC200816   EC202947   EC203144   EC203775   EC204207   EC205394   EC206665   EC213029   EC222209   EC224862   EC226362   EC238165   EC238194   EC238636   EC238968   EC246769   EC248641   EC249031   EC254656   EC254692   EC254735   EC255149   EC258196   EL874403   EL874404   EL874483   EL874484   EL874554   EL874555   EL874626   EL874627   GH942088   GH943661   GH948756   GH955170   GH956185   GH960008   GH961983   GH962177   GH967174   GH967980   GH968773   GH970928   GH972261   GH981106   GH981149   GH983441   GH983950   M63450 AAA29051   U87286
UniProtKB/Swiss-Prot	P28167
UniProtKB/TrEMBL	H9XVP0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   anterior endoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi central brain anlage in statu nascendi   (Fisher et al., 2012) hindgut anlage in statu nascendi   (Fisher et al., 2012) posterior ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7 -- 8   anterior endoderm anlage   (Fisher et al., 2012) central brain anlage   (Fisher et al., 2012) hindgut anlage   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) inclusive hindgut primordium   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 15   presumptive embryonic/larval central nervous system   (Lai et al., 1991) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic large intestine   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic stage 15   embryonic/larval hindgut   (Lai et al., 1991)
Additional Descriptive Data
	The zfh2 transcript distribution pattern corresponds closely to that of zfh2 protein. (Lai et al., 1991)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference larval stage   abdominal 3 ventro-lateral serotonergic neuron -- abdominal 6 ventro-lateral serotonergic neuron   (Lundell and Hirsh, 1992) embryonic/larval central nervous system   (Lundell and Hirsh, 1992) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system | lateral   (Lundell and Hirsh, 1992) embryonic stage 13 -- 15   presumptive embryonic/larval central nervous system   (Lai et al., 1991) embryonic stage 15   embryonic/larval hindgut   (Lai et al., 1991) embryonic stage 15 -- 17   EW2 neuron   (Lundell et al., 2003) EW3 neuron   (Lundell et al., 2003) embryonic stage 17   EW2 neuron   (Lundell and Hirsh, 1998) EW3 neuron   (Lundell and Hirsh, 1998) larval stage   abdominal 3 dorso-lateral neuroblast -- abdominal 7 dorso-lateral neuroblast   (Lundell and Hirsh, 1992) third instar larval stage   ventro-lateral serotonergic neuron | subset   (Lundell and Hirsh, 1998) dopaminergic neuron | subset   (Lundell and Hirsh, 1998)
Additional Descriptive Data
	zfh2 protein isexpressed in about 50 cells/segment in the lateral embryonic CNS atpositions consistent with the subsequent location of serotonin cells. Inlarvae, it is expressed in a dispersed pattern in about 20% of the cellsin the ventral ganglion. A subset of these also express Ddc and includedopamine and seratonin neurons. (Lundell and Hirsh, 1992) Markers that uniquely identify the cells of the NB3-7 lineage were used to examine the serotonin expressing cell lineages. (Lundell et al., 2003) zfh2 protein is first detected in the CNS at the middle of germ band retraction. It is present in at least 50 neurons per hemisegment as well as in cells of the brain. It is also detected at lower levels in the hindgut of older embryos (Lai et al., 1991) zfh2 is expressed in the lateral, but not the medial, of the paired ventro-lateral serotonergic neurons. (Lundell and Hirsh, 1998)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Lai et al., 1991)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    82.05 Larval Midgut    3.5 Larval Hindgut    42.3 Larval Malpighian Tubules    2 Larval Fat Body    4.9 Larval Salivary Gland    4.5 Larval Trachea    14.375 Larval Carcass    9 Adult Head    16 Adult Eye    4.6 Adult Brain    63.6 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    6.2 Adult Midgut    4.7 Adult Hindgut    4.6 Adult Malpighian Tubules    3 Adult Fat Body    8.8 Adult Salivary Gland    9.8 Adult Heart    0.65 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    1.3 Adult Testis    1.2 Adult Male Accessory Gland    3.4 Adult Carcass    1.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    16   NA  Eye    4.6   NA  Brain    63.6   82.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    45   NA  Crop    6.2   3.5  Midgut    4.7   42.3  Hindgut    4.6   2  Malpighian Tubules    3   4.9  Fat Body    8.8   4.5  Salivary Gland    9.8   NA  Heart    0.65   14.375  Trachea    NA   NA  VirginFemale Spermatheca    4.3   NA  InseminatedFemale Spermatheca    8.6   NA  Ovary    1.3   NA  Testis    1.2   NA  Male Accessory Gland    3.4   9  Carcass    1.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    3 head, mated 1-day male    8 head, mated 4-day male    9 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    2 carcass, larvae L3 wandering    12 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr    27 embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr  (27) embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr    27 embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr    27 embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr    27 embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr  27 embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr    27 embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    3 embryo 04-06hr    11 embryo 06-08hr    13 embryo 08-10hr    18 embryo 10-12hr    11 embryo 12-14hr    27 embryo 14-16hr    11 embryo 16-18hr    12 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3  (61) eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3  (61) eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    3 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    3 embryonic S1    13 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    61 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    14 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_11 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • GH11902 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004607 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1449-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for zfh2 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal zfh21-M707.R zfh22-M371.R lethal | pupal stage, with Scer\GAL4pnr-MD237 zfh2GD4506 partially lethal - majority die | pupal stage, with Scer\GAL4LP30 zfh2dsRNA.Scer\UAS viable zfh22-M390.R viable | poor zfh21-M707.R zfh22-M390.R zfh2MS209 Other Phenotypes Allele visible | adult stage A1, with Scer\GAL4LP30 zfh2dsRNA.Scer\UAS Phenotype manifest in Allele alula zfh2MS209 medial costal vein zfh2MS209 sclerite, with Scer\GAL4LP30 zfh2dsRNA.Scer\UAS wing, with Scer\GAL4LP30 zfh2dsRNA.Scer\UAS wing | proximal zfh22-M390.R zfh21-M707.R zfh2MS209 wing disc zfh2MS209 wing hinge, with Scer\GAL4LP30 zfh2dsRNA.Scer\UAS wing vein L1 zfh2MS209
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of zfh2 Class Mutagen Stocks Known lesion zfh25-HA-1838 1 -- zfh2c02999a 1 -- zfh2d09602 1 -- zfh2f05624 1 -- zfh2MS209 P-element activity 1 Yes zfh21-M707.R P-element activity 0 -- zfh22-M371.R P-element activity 0 -- zfh22-M390.R P-element activity 0 -- zfh2MS209R20 Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of zfh2 Class Mutagen Stocks Known lesion zfh2GD4506 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes zfh2T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP in vitro construct - coding region fusion 1 Yes zfh2dsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes zfh2KK100817 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(4)C1-7a (Sousa-Neves et al., 2005) Df(4)C1-10a (Sousa-Neves et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4506} NA P{KK100817} NA P{UAS-zfh2.RNAi} NA characterization construct PBac{zfh2-GFP.FPTB} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{hsp26-pt-T}zfh21-M707.R NA P{hsp26-pt-T}zfh22-M371.R NA P{hsp26-pt-T}zfh22-M390.R NA P{RS5}zfh25-HA-1838 NA PBac{PB}zfh2c02999a NA PBac{WH}zfh2f05624 NA insertion of mobile activating element P{XP}zfh2d09602 NA insertion of enhancer trap binary system P{GAL4}zfh2MS209 No
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
RNA polymerase II regulatory region sequence-specific DNA binding inferred from direct assay (Lundell and Hirsh, 1992)
Biological Process
CV term Evidence References
imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Whitworth and Russell, 2003) nervous system development inferred from expression pattern (Lai et al., 1991)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Lai et al., 1991)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 1994.10.1) traceable author statement (Lai et al., 1991) sequence-specific DNA binding transcription factor activity inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
central nervous system development non-traceable author statement (Swiss-Prot Project Members, 1994.10.1) regulation of transcription, DNA-dependent inferred from sequence or structural similarity with UniProtKB:Q15911 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with UniProtKB:Q15911 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1994.10.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions zfh2 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68628 DroID - A comprehensive database of gene and protein interactions. • FBgn0004607 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43795
Orthologs
OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HT9BG Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6NP5Z8 Arthropod inclusive ortholog search EOG65DV47 Metazoa inclusive ortholog search EOG6B61XR
Orthologs in Drosophila Species (EOG6HT9BG)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\zfh2     Drosophila simulans   Dsim\GD24406 Y   Drosophila sechellia   Dsec\GM26799     Drosophila erecta   Dere\GG16389 Y   Drosophila yakuba   Dyak\GE24295     Drosophila yakuba   Dyak\GE13066     Drosophila yakuba   Dyak\GE14550 Y   Drosophila ananassae   Dana\GF19021 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA13024 Y   Drosophila persimilis   Dper\GL18142 Y   Drosophila willistoni   Dwil\GK13603 Y   Drosophila virilis   Dvir\GJ19746 Y   Drosophila virilis   Dvir\GJ12809     Drosophila virilis   Dvir\GJ16768     Drosophila mojavensis   Dmoj\GI18747     Drosophila mojavensis   Dmoj\GI14048 Y   Drosophila grimshawi   Dgri\GH24013 Y   Drosophila grimshawi   Dgri\GH11444
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6NP5Z8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB54030   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG017648   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19896   Atta cephalotes Leafcutter ant  Acep\ACEP22224   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18476   Camponotus floridanus Florida carpenter ant  Cflo\CFLO11386   Camponotus floridanus Florida carpenter ant  Cflo\CFLO24523   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18944   Linepithema humile Argentine ant  Lhum\LH22749   Pogonomyrmex barbatus Red harvester ant  Pbar\PB23462   Solenopsis invicta Red fire ant  Sinv\SINV19129   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002526   Bombyx mori Silkmoth  Bmor\BGIBMGA012021   Pediculus humanus Human body louse  Phum\PHUM190870   Tribolium castaneum Red flour beetle  Tcas\zfh2
Orthologs in non-Insect Arthropods (EOG65DV47)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_35537   Daphnia pulex Water flea  Dpux\JGI_V11_233159   Ixodes scapularis Deer tick  Isca\ISCW017915
Orthologs in non-Arthropod Metazoa (EOG6B61XR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ZC123.3
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24406 Drosophila erecta Dere\GG16389 Drosophila yakuba Dyak\GE14550 Drosophila ananassae Dana\GF19021 Drosophila pseudoobscura pseudoobscura Dpse\GA13024 Drosophila persimilis Dper\GL18142 Drosophila willistoni Dwil\GK13603 Drosophila virilis Dvir\GJ19746 Drosophila mojavensis Dmoj\GI14048 Drosophila grimshawi Dgri\GH24013
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	38674 w1118; PBac{zfh2-GFP.FPTB}VK00033/TM3, Sb1 25676 w*; P{GAL4}zfh2MS209/In(4)ciD, ciD panciD
Harvard	- P{XP}zfh2d09602 - PBac{WH}zfh2f05624 - PBac{PB}zfh2c02999a, PBac{PB}c02999b
Kyoto	125424 w1118; P{RS5}zfh25-HA-1838
VDRC	v13305 w1118; P{GD4506}v13305/TM3
Genomic Clones ( 2 )
	BACH57F14 BACR44L03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 103 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH11902
Other clones	IP22195
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH26340 SD09261
Other clones	28567 38481 39539 39716 64287 65789 66421 76189 78284 79415 80118 82176 101003 108544 118272 121326 122260 124287 147348 153348 153962 155797 163588 164845 164990 174424 214245.21 214257.19 214275.4 214309.15 214511.13 281783 CK01.10D.E9 CK01.30B.B2 CK01.41B.C1 CK01.71B.B2 CK01.127D.C12 CK01.128D.H10 EC051878 EC053951 EC054366 EC056074 EK050743 EK056637 EK098633 EK099763 EK136632 EK151619 EK153253 EK166163 EK176536 EK176563 EK189606 EK189964 EK206951 EK212140 EK212330 EK223728 EK235110 EK245545 EK246847 EK251461 EK257020 EK258529 EK267864 EK269423 EK270652 EK279306 EK288303 EK291508 EK307131 EP07703 EP11135 EP11234 EP11750 EP12416 EP19964 IP14419 IP14519 IP14619 IP14719 RP004084106 RP004102109 RP004193939 RP004204471 RP004210182 RP004233696 RP004235012 RP004248600 RP004260003 RP004400454 RP004420435 RP004450445 RP004460696 RP004471435 RP004528179 RP004528972 RP004550188 SD04877
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004607 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43795
Antibody Information
	polyclonal (Lai et al., 1991, Lundell and Hirsh, 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: zfh2 CG1449 (Zhong, 1999.11)
Source for database merge of
Additional comments
Other Comments
	zfh2 is required for proximal wng development. (Whitworth and Russell, 2003) eg is necessary for the maintenance of en and zfh2 expression in the serotonin neurons. (Lundell and Hirsh, 1998) One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus Engrailed binding site. (Kalionis and O'Farrell, 1993) Assayed in an analysis of chromosome 4 using pulsed field gel electrophoresis. (Locke and McDermid, 1993) Profile of zfh2 expression in the larval CNS shows intriguing overlap with Ddc expression in specific serotonin and dopamine neurons. The product of zfh2 binds to a protein binding site in the Ddc promoter. (Lundell and Hirsh, 1992) The product of zfh2 possesses three homeodomains and sixteen C2H2 zinc fingers. The novel arrangement of interspersed homeodomain and zinc-finger motifs in the primary sequences of the zfh1 and zfh2 gene products may signify an unusual mechanism of transcriptional regulation by these products. (Fortini et al., 1991) The expression pattern of zfh2 suggests that the protein may be involved in neurogenesis. (Lai et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079884 NM_001258496 NM_001272130 RefSeq (Proteins) • NP_524623 NP_001245425 NP_001259059 DDBJ / EMBL / GenBank • AAA29051 AAF59339 AAF59339.2 AAL28229 AAQ67630 AAQ67631 AAQ67632 AAQ67633 AAQ67634 AAQ67635 AAQ67636 AAQ67637 AAQ67638 AAQ67639 AAQ67640 AAQ67641 AAQ67642 AAQ67643 AAQ67644 AAQ67645 AAQ67646 AAQ67647 AAQ67648 AAQ67649 AAQ67650 AAQ67651 AAQ67652 AAQ67653 AC009535 AC010668 AC018478 AF242879 AF242880 AFH06785 AGB96549 AI134433 AW940433 AY060681 AY312816 AY312817 AY312818 AY312819 AY312820 AY312821 AY312822 AY312823 AY312824 AY312825 AY312826 AY312827 AY312828 AY312829 AY312830 AY312831 AY312832 AY312833 AY312834 AY312835 AY312836 AY312837 AY312838 AY312839 M63450 U87286 Entrez Gene - A searchable database of RefSeq genes. • 43795 UniProtKB/Swiss-Prot • P28167 UniProtKB/TrEMBL • H9XVP0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Homeobox, conserved site (IPR017970) Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087) Zinc finger, C2H2-like (IPR015880) Homeodomain (IPR001356) Zinc finger, C2H2 (IPR007087) Homeodomain-like (IPR009057) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH11902
Linkouts
	BioGRID - A database of protein and genetic interactions • 68628 DroID - A comprehensive database of gene and protein interactions. • FBgn0004607 DRSC - Results from RNAi screens. • FBgn0004607 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004607 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1449-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004607 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43795 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/zcfhm21.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43795 modMine - Data generated by the modENCODE project. • FBgn0004607
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	CG1449 (Keleman et al., 2009.8.5) zfh2 (Riddle et al., 2008, Tran and Doe, 2007, Stark et al., 2005, Podemski et al., 2004, de Navascués and Modolell, 2007, Akdemir et al., 2007, Johansson et al., 2007, Terriente et al., 2008, Weake et al., 2011, Perea et al., 2009, Dichtel-Danjoy et al., 2009, Tran et al., 2010, Spokony and White, 2012.5.22, Burgler and Macdonald, 2005, Sun et al., 2004, Beltran et al., 2007, Davis et al., 2011, Touma et al., 2012) Zfh-2 (Mettler et al., 2006, Lundell et al., 2003, Isshiki et al., 2001, Karcavich and Doe, 2005, Benítez et al., 2009) zfh-2 (Sousa-Neves et al., 2005, Vogler and Urban, 2004, Baena-Lopez et al., 2003, Whitworth and Russell, 2003, Kramps et al., 2002, Podemski et al., 2001, Bowles et al., 2000, Leach et al., 2000, Locke et al., 2000, Whitworth and Russell, 2000, Locke et al., 1999, Locke et al., 1998, Lundell and Hirsh, 1998, Ahmed and Podemski, 1997, Lundell and Hirsh, 1993, Kalionis and O'Farrell, 1993, Hashimoto et al., 1992, Lai et al., 1991, Fortini et al., 1991, Guntur and Lundell, 2006, Guntur and Lundell, 2008, Dichtel-Danjoy et al., 2009) Zfh2 (Vogler and Urban, 2003, Kohwi et al., 2011, Nusinow et al., 2008, Tran and Doe, 2008, Spresser et al., 2008, Beckervordersandforth et al., 2008, Slawson et al., 2006) ZFH-2 (Lundell and Hirsh, 1994) ZFH2 (Kanai et al., 2005)
Name Synonym	Zinc finger homeodomain-2 (Guntur and Lundell, 2008) zinc finger homeodomain 2 (Perea et al., 2009, Terriente et al., 2008) Zn finger homeodomain 2 (Davis et al., 2011)
Secondary FlyBase IDs
References ( 91 )
Generate a list of	All references relating to this gene ( 91 )
List References by type	Research paper  ( 60 ) Supplementary material  ( 4 ) Review  ( 1 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 9 ) FlyBase analysis  ( 2 ) Letter  ( 2 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 ) DNA/RNA sequence record  ( 2 )
Recent research papers ( 5 )
	Touma et al., 2012, Development 139(4): 657--666 Drosophila Polycomb complexes restrict neuroblast competence to generate motoneurons. [FBrf0217274] Davis et al., 2011, BMC Dev. Biol. 11: 66 Identification of common and cell type specific LXXLL motif EcR cofactors using a bioinformatics refined candidate RNAi screen in Drosophila melanogaster cell lines. [FBrf0216830] Gabilondo et al., 2011, Mech. Dev. 128(3-4): 208--221 A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Kohwi et al., 2011, Development 138(9): 1727--1735 The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity. [FBrf0213466] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...