In the wing pouch, rho is expressed in presumptive wing veins L3, L4, and L5, and in two stripes parallel to the D/V compartment boundary. Expression is also observed in the presumptive wing hinge and mesonotum regions. No transcript is observed in the haltere disc. rho transcript is expressed in the presumptive hinge region of the haltere disc, but not in the haltere pouch.

rho expression is first observed in the leg disc at 86 hr AEL in a central domain and in concentric rings. Expression is weak until 96 hr AEL at which point the central domain is substituted by a small cluster of cells in the ventral pretarsus, in the pit of cells that give rise to the tendon. The concentric rings of expression are located in the presumptive tibea, tibia/femur boundary, and trochanter positions. By 120 hr AEL, the doral femoral cluster has become a twin cluster and there is now a ring in every presumptive leg segment. Pretarsal expression has disappeared. This pattern persists at 8 hr APF, when the rings of expression can be seen as stripes in the everted leg with additional isolated expressing cells in the pretarsus. Each rho stripe saddles the fold between adjacent segments, and has a graded intensity, which is weaker proximally and stronger distally.

rho transcript is expressed in concentric ring pattern in leg discs.

Expression pattern inferred from unspecified enhancer trap line.

As the Malphighian tubules start to evert, rho transcript is detected in the tip mother cell, and subsequently in the tip cell.

rho is expressed in follicle cells and in the germline during oogenesis. At oogenesis stage S9, it is expressed in a broad group of cells in the dorsal-anterior end of the egg chamber and by stage S10, expression is restricted to two dorsal-anterior stripes corresponding to the sites of future dorsal respiratory appendages. The early and late oogenesis expression patterns are expanded in fs(1)K10 mutants. In grk and FBgn0003731:Egfr mutants, the early oogenisis pattern is unaffected but the late pattern shows severe restriction of cells expressing FBgn0004635:rho @.

rho is expressed in wandering third instar larvae and in early prepupae in a pattern of intersecting stripes that is likely to be the wing vein primordia. Later, its expression is restricted to developing veins.

rho transcripts are detected in embryos, pupae, and adults on northern blots.

rho is expressed in a complex pattern during embryogenesis. Expression is first observed at the cellular blastoderm stage in two ventrolateral domains, 7-8 cells wide separated by a 13-15 cell wide unlabeled ventral strip. These domains become narrower and modulated in intensity along the A/P axis. As the mesoderm invaginates, the label moves ventrally and becomes restricted to a single row of cells on either side of the midline. Expression continues in these cells as they meet to form the mesectoderm and persists until germ band retraction. During germ band extension, strong expression is seen in cells that will form tracheal pits and in a single large cell dorsal and posterior to the tracheal pit that is thought to be the precursor of chordotonal organs. During germ band retraction, expression begins in the CNS in a segmentally repeated pattern. At the end of germ band retraction, cells forming the anterior-most row in each abdominal segment are labelled both dorsally and ventrally. In the thoracic segments, only the dorsal cells are labelled.

ve protein is localized predominantly on the apical surface of the dorsal anterior follicle cells.

ve protein is expressed in the germline and in dorsal anterior follicle cells during oogenesis. In early stages of oogenesis, it is expressed in a broad group of cells in the dorsal-anterior end of the egg chamber and in later stages, expression is restricted to two dorsal-anterior stripes

ve protein is expressed in the third instar larval eye disc posterior to the morphogenetic furrow. Staining is restricted to the ommatidia and occurs mainly in receptor cells R2, R5, and R8.