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Gene Dmel\rho

General Information
Symbol	Dmel\rho	Species	D. melanogaster
Name	rhomboid	Annotation symbol	CG1004
Feature type	protein_coding_gene	FlyBase ID	FBgn0004635
Gene Model Status	Current	Stock availability	59 publicly available
Also Known As	ve, Rho1, Rho-1, DMRHOb, DMRHO
Genomic Location
Chromosome (arm)	3L	Recombination map	3-0.2
Cytogenetic map	62A2-62A2	Sequence location	3L:1,463,792..1,468,475 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene rhomboid is referred to in FlyBase by the symbol Dmel\rho (CG1004, FBgn0004635). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: serine-type endopeptidase activity. There is experimental evidence for 16 unique biological process terms, many of which group under: anatomical structure development; biological regulation; post-embryonic appendage morphogenesis; organ development; post-embryonic organ morphogenesis; response to chemical stimulus; learning or memory; cellular component organization or biogenesis; cell division; associative learning. 68 alleles are reported. The phenotypes of these alleles are annotated with: hypodermal muscle of larval abdomen; organ system subdivision; organ system; A1-7 oblique muscle; adult segment; larval abdominal segment 5; embryonic/larval neuron; primordium; A1-7 ventral oblique muscle 1; nervous system. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase S54, rhomboid; Peptidase S54, rhomboid domain; Peptidase S54, rhomboid, metazoan. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1635462_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of rho. Gene sequence location is 3L:1463792..1468475.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ve: veinlet (E. Bier) ve: veinlet From Duncan, 1935, Am. Naturalist 69: 94-96. Viable alleles exhibit wing venation defects; strong alleles are embryonic lethal. In flies homozygous for viable alleles the L3, L4, and L5 veins do not reach the wing margins (Duncan; Waddington). Developmentally, veins appear complete in prepupa but distal tips are obliterated during the contraction period (Waddington, 1939, 1940). The shortened-vein phenotype is suppressed by px (Waddington), net, and su(ve), and is enhanced by vn, H, Ax, ci, tg2, and ri (Waddington; Diaz-Benjumea and Garcia-Bellido, 1990, Wilhelm Roux's Arch. Dev. Biol 198: 336-54.). Vein-specific modifiers, such as gp, (Bridges and Morgan, 1919, Carnegie Inst. Washington Publ. No. 278: 208) or PL(2)L4a (Thompson, 1976), interact with the effect of ve on L4. The L5 vein seldom extends beyond the posterior crossvein. ve2 is a stronger allele, in which the L2 is also affected (Bertschmann); L2 vein occasionally complete (Thompson, 1976), but other veins do not overlap wild type. Distribution of sense organs (campaniform sensilla and bristles) on L3 is shifted proximally in ve (Spivey and Thompson) When a ve stock is selected for shortened veins, the F1 produced by mating wild-type males to mutant females show terminal gaps in L5 (Thompson and Thoday, 1976). ve/ve/+ intersexes are veinlet, whereas ve/ve/+ triploids are normal, according to Pipkin. Interestingly flies heterozygous for ve and strong embryonic lethal alleles display less severe veinlet phenotypes than ve homozygotes (Bier et al.; Diaz-Benjumea and Garcia-Bellido); furthermore, ve1/ve5 flies appear wild type (Bier, unpublished). Homozygous ve5 embryos exhibit three major types of defects: (1) Dorsoventral defects: Embryos exhibit a deletion of epithelial cells derived from a ventrolateral strip of the blastoderm fate map (i.e., loss of mediolateral cuticular denticles and sensory structures). Other phenotypes resulting from blastoderm patterning defects include failure to complete dorsal closure and development of an abnormal pointed head skeleton (Jurgens et al.; Mayer and Nusslein-Volhard). (2) Midline defects: Mesectodermal cells giving rise to glia and unpaired neurons are abnormal or fail to form. Late developmental consequences include a narrower CNS and pathfinding abnormalities (Mayer and Nusslein-Volhard). (3) Peripheral-nervous-system defects: Two stretch receptor organs (lateral abdominal chordotonal organs) fail to form in lethal ve mutants. The primary chordotonal-organ-precursor cells are likely to be affected since the four progeny sensory-organ cells derived from that precursor cell are missing as a group (Bier et al.). Other late embryonic defects include loss of longitudinal body-wall muscles, ventrally displaced muscle-attachment sites (Bier et al.), and loss of the first row of denticles in abdominal segments (Mayer and Nusslein-Volhard).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms proteolysis References Uhl et al., 2010, Dev. Biol. 343(1-2): 154--166 Marco et al., 2009, Bioinformatics 25(19): 2473--2477 Wojcinski et al., 2011, Dev. Biol. 358(1): 168--180
FB2012_01	Sequence features HFA08734 BKN31378 Unknown in situ Controlled Vocabulary Terms denticle field anlage epidermis oogenesis membrane-bounded vesicle tracheal primordium peptide bond cleavage involved in epidermal growth factor receptor ligand maturation negative regulation of gene expression References Rousso et al., 2010, Development 137(20): 3427--3437 Yasugi et al., 2010, Development 137(19): 3193--3203 Ozdemir et al., 2011, Genome Res. 21(4): 566--577 McHale et al., 2011, Dev. Biol. 360(1): 230--240 Sukhanova et al., 2011, Dev. Biol. 359(2): 190--198
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 62A2-62A2   Limits computationally determined from genome sequence between P{EP}rhoEP3704&P{PZ}l(3)0622606226 and P{PZ}dlt04276
Experimentally Determined Cytological Location
Cytogenetic map Notes References 62A-62A   (determined by in situ hybridisation)   (Bier et al., 1990)
Experimentally Determined Recombination Data
Location	3-0.2   3-3 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)	ru (Roberts and Evans-Roberts, 1979, Robertson and Riviera, 1972)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\rho for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) rho-RA FBtr0072694  NM_079159   2550   355
Additional Transcript Data & Comments
Reported size (kB)	2.9, 2.5 (northern blot) (Bier et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein rho-PA   FBpp0072578   39.3   355   5.12     NP_523883   AAF47496
Additional Polypeptide Data & Comments
Reported size (kDa)	355 (aa); 39 (kD) (Bier et al., 1990)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S54, rhomboid (IPR002610) Peptidase S54, rhomboid, metazoan (IPR017213) Peptidase S54, rhomboid domain (IPR022764)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA247028   AE014296 AAF47496   AF067877   AF067878   AW941611   AY121641 AAM51968   BI372537   BI592257   BI592434   BI615764   BI629788   CK662486   CO186338   CO187577   CO282877   CO310638   CO338653   CZ478728   EC052343   EC053735   EC054759   EC054858   EC055330   EC057180   EC067192   EC244260   EC245491   EC264421   FE426494   FE426549   FE426564   FE863142   GH740930   GH745245   GH762617   GH763580   GH767332   GH768067   GH768150   GH768224   GH769223   GH769231   X52454 CAA36692
UniProtKB/Swiss-Prot	P20350
UniProtKB/TrEMBL	Q540V7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000160136 3L:1,461,873..1,461,882 bound_moiety=sna-XP comment=sna-binding site s1 evidence=experimental (Bergman et al., 2004, Ip et al., 1992) protein binding site FBsf0000160137 3L:1,461,943..1,461,952 bound_moiety=sna-XP comment=sna-binding site s2 evidence=experimental (Ip et al., 1992, Bergman et al., 2004) protein binding site FBsf0000160138 3L:1,461,978..1,461,988 bound_moiety=dl-XP comment=dl-binding site d2 evidence=experimental (Bergman et al., 2004, Ip et al., 1992) protein binding site FBsf0000160139 3L:1,462,012..1,462,021 bound_moiety=twi-XP comment=tll-binding site t1 evidence=experimental bound_moiety=tll-XP (Bergman et al., 2004, Ip et al., 1992) protein binding site FBsf0000160140 3L:1,462,023..1,462,032 comment=tll-binding site t2 evidence=experimental bound_moiety=tll-XP bound_moiety=twi-XP (Bergman et al., 2004, Ip et al., 1992) protein binding site FBsf0000160141 3L:1,462,024..1,462,033 bound_moiety=sna-XP comment=sna-binding site s4 evidence=experimental (Bergman et al., 2004, Ip et al., 1992) protein binding site FBsf0000160142 3L:1,462,037..1,462,047 bound_moiety=dl-XP comment=dl-binding site d3 evidence=experimental (Bergman et al., 2004, Ip et al., 1992) protein binding site FBsf0000160135 3L:1,461,842..1,461,852 bound_moiety=dl-XP comment=dl-binding site d1 evidence=experimental (Bergman et al., 2004, Ip et al., 1992) protein binding site FBsf0000160246 3L:1,462,084..1,462,094 bound_moiety=dl-XP evidence=experimental (Ip et al., 1992, Bergman et al., 2004) protein binding site FBsf0000160247 3L:1,461,963..1,461,973 evidence=experimental bound_moiety=sna-XP (Bergman et al., 2004, Ip et al., 1992) regulatory region FBsf0000161259 3L:1,461,606..1,462,199 comment=600 base rho neuroectoderm element (NEE). evidence=experimental linked_to=HindIII-AvaII_rfrag (Ip et al., 1992) regulatory region FBsf0000161260 3L:1,461,806..1,462,138 comment=minimal rho neuroectoderm element (NEE). evidence=experimental linked_to=StyI-XhoI_rfrag (Ip et al., 1992) regulatory region FBsf0000161261 3L:1,461,864..1,461,869 comment=E box evidence=experimental (Ip et al., 1992) regulatory region FBsf0000161262 3L:1,461,887..1,461,892 comment=E box evidence=experimental (Ip et al., 1992) regulatory region FBsf0000161263 3L:1,461,967..1,461,972 comment=E box evidence=experimental (Gray et al., 1994) regulatory region FBsf0000161264 3L:1,462,124..1,462,487 comment=The minimal rho midline element (MLE) directs expression in the mesectoderm and gives weak expression in the tracheal pits. evidence=experimental linked_to=EcoRI-SspI_rfrag (Ip et al., 1992) regulatory region FBsf0000161265 3L:1,462,124..1,462,879 comment=The rho midline element (MLE) directs all aspects of the late embryonic rho expression pattern including expression in the mesectoderm and tracheal pits. evidence=experimental linked_to=EcoRI-EcoRI_rfrag (Ip et al., 1992)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference oogenesis | late   follicle cell   (Ruohola-Baker et al., 1993) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   Malpighian tubule tip cell   (Kerber et al., 1998) tracheal pit   (Bier et al., 1990) chordotonal organ | precursor   (Bier et al., 1990) embryonic stage 5   organism | restricted & ventro-lateral   (Bier et al., 1990) Comment:ventro-lateral stripe organism | ventro-lateral   (Ponomareff et al., 2001) Comment:in a stripe 7-8 cells wide embryonic stage 6 -- 7   mesectoderm   (Bier et al., 1990) embryonic stage 7 -- 12   ventral midline   (Bier et al., 1990) embryonic stage 10 -- 15   embryonic central brain | subset   (Page, 2003) embryonic foregut | anterior & ventral   (Page, 2003) embryonic stage 11 -- 13   epidermis | segmentally repeated   (Alexandre et al., 1999) denticle field anlage   (Alexandre et al., 1999) embryonic stage 12 -- 17   central nervous system | segmentally repeated   (Bier et al., 1990) embryonic stage 13 -- 17   organism | segment boundary   (Bier et al., 1990) larval stage & pupal stage   wing vein L2 | precursor   (Sturtevant et al., 1993) wing vein L4 | precursor   (Sturtevant et al., 1993) wing vein | precursor   (Sturtevant et al., 1993) wing vein L3 | precursor   (Sturtevant et al., 1993) wing vein L5 | precursor   (Sturtevant et al., 1993) wing vein L1 | precursor   (Sturtevant et al., 1993) pupal stage   wing vein L5   (Sturtevant et al., 1993) wing vein L3   (Sturtevant et al., 1993) wing vein L4   (Sturtevant et al., 1993) wing vein L1   (Sturtevant et al., 1993) crossvein   (Sturtevant et al., 1993) wing vein   (Sturtevant et al., 1993) wing vein L2   (Sturtevant et al., 1993) pupal stage P6   wing vein   (Verheyen et al., 2001) Comment:25-30 hr APF northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & pupal stage & adult stage     (Bier et al., 1990)
Additional Descriptive Data
	rho is expressed in a complex pattern during embryogenesis. Expression is first observed at the cellular blastoderm stage in two ventrolateral domains, 7-8 cells wide separated by a 13-15 cell wide unlabeled ventral strip. These domains become narrower and modulated in intensity along the A/P axis. As the mesoderm invaginates, the label moves ventrally and becomes restricted to a single row of cells on either side of the midline. Expression continues in these cells as they meet to form the mesectoderm and persists until germ band retraction. During germ band extension, strong expression is seen in cells that will form tracheal pits and in a single large cell dorsal and posterior to the tracheal pit that is thought to be the precursor of chordotonal organs. During germ band retraction, expression begins in the CNS in a segmentally repeated pattern. At the end of germ band retraction, cells forming the anterior-most row in each abdominal segment are labelled both dorsally and ventrally. In the thoracic segments, only the dorsal cells are labelled. (Bier et al., 1990) rho transcripts are detected in embryos, pupae, and adults on northern blots. (Bier et al., 1990) rho is expressed in wandering third instar larvae and in early prepupae in a pattern of intersecting stripes that is likely to be the wing vein primordia. Later, its expression is restricted to developing veins. (Sturtevant et al., 1993) As the Malphighian tubules start to evert, rho transcript is detected in the tip mother cell, and subsequently in the tip cell. (Kerber et al., 1998) rho is expressed in follicle cells and in the germline during oogenesis. At oogenesis stage S9, it is expressed in a broad group of cells in the dorsal-anterior end of the egg chamber and by stage S10, expression is restricted to two dorsal-anterior stripes corresponding to the sites of future dorsal respiratory appendages. The early and late oogenesis expression patterns are expanded in fs(1)K10 mutants. In grk and FBgn0003731:Egfr mutants, the early oogenisis pattern is unaffected but the late pattern shows severe restriction of cells expressing FBgn0004635:rho@. (Ruohola-Baker et al., 1993)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   photoreceptor cell R8 | presumptive   (Freeman et al., 1992) photoreceptor cell R2 | presumptive   (Freeman et al., 1992) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11   tracheal primordium   (Llimargas and Casanova, 1999) third instar larval stage   ommatidium   (Freeman et al., 1992) photoreceptor cell R5 | presumptive   (Freeman et al., 1992) photoreceptor cell R2   • membrane-bounded vesicle (Freeman et al., 1992) photoreceptor cell R5   • membrane-bounded vesicle (Freeman et al., 1992) photoreceptor cell R8   • membrane-bounded vesicle (Freeman et al., 1992) adult stage & oogenesis   follicle cell   (Ruohola-Baker et al., 1993)
Additional Descriptive Data
	rho protein is localized predominantly on the apical surface of the dorsal anterior follicle cells. (Ruohola-Baker et al., 1993) rho protein is expressed in the third instar larval eye disc posterior to the morphogenetic furrow. Staining is restricted to the ommatidia and occurs mainly in receptor cells R2, R5, and R8. (Freeman et al., 1992)
Marker for
Subcellular Localization
CV Term	plasma membrane (Freeman et al., 1992)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0004635    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0004635 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0004635 expression level Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    1495 embryo 04-06hr    1332 embryo 06-08hr    1880 embryo 08-10hr    1637 embryo 10-12hr    2432 embryo 12-14hr    1633 embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr  (1495) embryo 04-06hr  (1332) embryo 06-08hr  (1880) embryo 08-10hr  (1637) embryo 10-12hr  (2432) embryo 12-14hr  (1633) embryo 14-16hr  (1083) embryo 16-18hr  (862) embryo 18-20hr  (839) embryo 20-22hr  (331) embryo 22-24hr  (451) larva L1  (342) larva L2  (225) larva L3 12hr old  (232) larva L3 puffstage 1-2  (166) larva L3 puffstage 3-6  (312) larva L3 puffstage 7-9  (467) white prepupae new  (488) white prepupae 12hr  (856) white prepupae 24hr  (646) pupae 2d postWPP  (647) pupae 3d postWPP  (294) pupae 4d postWPP  (357) adult male 01day  (253) adult male 05day  (253) adult male 30day  (256) adult female 01day  (184) adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr  1495 embryo 04-06hr    1332 embryo 06-08hr  (1880) embryo 08-10hr  (1637) embryo 10-12hr  (2432) embryo 12-14hr  (1633) embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    1495 embryo 04-06hr    1332 embryo 06-08hr    1880 embryo 08-10hr    1637 embryo 10-12hr    2432 embryo 12-14hr    1633 embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    1495 embryo 04-06hr    1332 embryo 06-08hr    1880 embryo 08-10hr    1637 embryo 10-12hr    2432 embryo 12-14hr    1633 embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0004635 expression level Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    1495 embryo 04-06hr    1332 embryo 06-08hr    1880 embryo 08-10hr    1637 embryo 10-12hr    2432 embryo 12-14hr    1633 embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr  (1495) embryo 04-06hr  (1332) embryo 06-08hr  (1880) embryo 08-10hr  (1637) embryo 10-12hr  (2432) embryo 12-14hr  (1633) embryo 14-16hr  (1083) embryo 16-18hr  (862) embryo 18-20hr  (839) embryo 20-22hr  (331) embryo 22-24hr  (451) larva L1  (342) larva L2  (225) larva L3 12hr old  (232) larva L3 puffstage 1-2  (166) larva L3 puffstage 3-6  (312) larva L3 puffstage 7-9  (467) white prepupae new  (488) white prepupae 12hr  (856) white prepupae 24hr  (646) pupae 2d postWPP  (647) pupae 3d postWPP  (294) pupae 4d postWPP  (357) adult male 01day  (253) adult male 05day  (253) adult male 30day  (256) adult female 01day  (184) adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr  1495 embryo 04-06hr    1332 embryo 06-08hr  1880 embryo 08-10hr  1637 embryo 10-12hr  2432 embryo 12-14hr  1633 embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    1495 embryo 04-06hr    1332 embryo 06-08hr    1880 embryo 08-10hr    1637 embryo 10-12hr    2432 embryo 12-14hr    1633 embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    1495 embryo 04-06hr    1332 embryo 06-08hr    1880 embryo 08-10hr    1637 embryo 10-12hr    2432 embryo 12-14hr    1633 embryo 14-16hr    1083 embryo 16-18hr    862 embryo 18-20hr    839 embryo 20-22hr    331 embryo 22-24hr    451 larva L1    342 larva L2    225 larva L3 12hr old    232 larva L3 puffstage 1-2    166 larva L3 puffstage 3-6    312 larva L3 puffstage 7-9    467 white prepupae new    488 white prepupae 12hr    856 white prepupae 24hr    646 pupae 2d postWPP    647 pupae 3d postWPP    294 pupae 4d postWPP    357 adult male 01day    253 adult male 05day    253 adult male 30day    256 adult female 01day    184 adult female 05day    80 adult female 30day    83 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0004635    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0004635 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0004635 expression level Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0004635 expression level Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    110.5 Larval Midgut    13.6 Larval Hindgut    65.6 Larval Malpighian Tubules    10.8 Larval Fat Body    5.2 Larval Salivary Gland    9 Larval Trachea    46.575 Larval Carcass    190.95 Adult Head    133.3 Adult Eye    109.45 Adult Brain    262.9 Adult Thoracic-Abdominal Ganglion    355.8 Adult Crop    23.8 Adult Midgut    17.8 Adult Hindgut    18.6 Adult Malpighian Tubules    9.5 Adult Fat Body    26 Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    38.5 Adult InseminatedFemale Spermatheca    61.1 Adult Ovary    4.3 Adult Testis    5.6 Adult Male Accessory Gland no informative data Adult Carcass    33.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    133.3   NA  Eye    109.45   NA  Brain    262.9   110.5  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    355.8   NA  Crop    23.8   13.6  Midgut    17.8   65.6  Hindgut    18.6   10.8  Malpighian Tubules    9.5   5.2  Fat Body    26   9  Salivary Gland    no informative data   NA  Heart    no informative data   46.575  Trachea    NA   NA  VirginFemale Spermatheca    38.5   NA  InseminatedFemale Spermatheca    61.1   NA  Ovary    4.3   NA  Testis    5.6   NA  Male Accessory Gland    no informative data   190.95  Carcass    33.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004635 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1004-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal rho7 rho8 rho9 rho10 rho11 rhodsRNA.cUa lethal, with Scer\GAL4en-e16E rhoEP3704 lethal | embryonic stage rhodsRNA.cLa lethal | embryonic stage | recessive rho5 rho6 rho7M43 rhoPΔ5 rhoPΔ16 rhoPΔ38 lethal | recessive rhoL68 viable rhove-1 rhove-2 viable, with Scer\GAL4sca-4512 rhoEP3704 Other Phenotypes Allele chemical sensitive rhoiks flightless | recessive rhounspecified learning defective rhoiks memory defective rhoiks neuroanatomy defective rho5 rhounspecified visible rho6 rhoGMR.Exel rhohs.PSt rhohs.sev rhoMod rhoStg rhove-1 rhoWk visible, with Scer\GAL4Bx-MS1096 rhoScer\UAS.cBa rhoScer\UAS.cdCa rhoScer\UAS.cUa rhoΔN.Scer\UAS visible, with Scer\GAL4CY2 rhoScer\UAS.cdCa visible, with Scer\GAL4ey.PH rhoEP3704 visible, with Scer\GAL4GMR.PF rhoScer\UAS.cdCa visible, with Scer\GAL4hs.2sev rhoEP3704 visible | dominant rhohs.PSt rhoKG07115 rhoMod rhoSld rhoStg rhoWk visible | heat sensitive rhohs.PSt visible | recessive rho9 rhoStg rhove-1 rhoWk Phenotype manifest in Allele A1-7 ventral acute muscle 2 | precursor rhoPΔ38 abdominal 1 lateral pentascolopidial chordotonal organ lch5 rho6 rhohs.PSt abdominal 1 ventral denticle belt rhounspecified abdominal 1 ventral longitudinal muscle 4 rho5 abdominal 1 ventral monoscolopidial chordotonal organ vch1 rho6 rhohs.PSt abdominal 1 ventral oblique muscle 1 rho5 abdominal 1 ventral oblique muscle 2 rho5 abdominal 2 dorsal acute muscle 1 rho7M43 abdominal 2 dorsal acute muscle 3 rho5 abdominal 2 dorsal oblique muscle 3 rho5 abdominal 2 dorsal oblique muscle 4 rho5 abdominal 2 lateral longitudinal muscle 1 rho5 abdominal 2 lateral pentascolopidial chordotonal organ lch5 rho6 rhohs.PSt abdominal 2 ventral longitudinal muscle 4 rho5 abdominal 2 ventral monoscolopidial chordotonal organ vch1 rho6 rhohs.PSt abdominal 2 ventral oblique muscle 1 rho5 abdominal 2 ventral oblique muscle 2 rho5 abdominal 3 dorsal acute muscle 1 rho7M43 abdominal 3 dorsal acute muscle 3 rho5 abdominal 3 dorsal oblique muscle 3 rho5 abdominal 3 dorsal oblique muscle 4 rho5 abdominal 3 lateral longitudinal muscle 1 rho5 abdominal 3 lateral pentascolopidial chordotonal organ lch5 rho6 rhohs.PSt abdominal 3 ventral longitudinal muscle 4 rho5 abdominal 3 ventral monoscolopidial chordotonal organ vch1 rho6 rhohs.PSt abdominal 3 ventral oblique muscle 1 rho5 abdominal 3 ventral oblique muscle 2 rho5 abdominal 4 dorsal acute muscle 1 rho7M43 abdominal 4 dorsal acute muscle 3 rho5 abdominal 4 dorsal oblique muscle 3 rho5 abdominal 4 dorsal oblique muscle 4 rho5 abdominal 4 lateral longitudinal muscle 1 rho5 abdominal 4 lateral pentascolopidial chordotonal organ lch5 rho6 rhohs.PSt abdominal 4 ventral denticle belt rhounspecified abdominal 4 ventral longitudinal muscle 4 rho5 abdominal 4 ventral monoscolopidial chordotonal organ vch1 rho6 rhohs.PSt abdominal 4 ventral oblique muscle 1 rho5 abdominal 4 ventral oblique muscle 2 rho5 abdominal 5 dorsal acute muscle 1 rho7M43 abdominal 5 dorsal acute muscle 3 rho5 abdominal 5 dorsal oblique muscle 3 rho5 abdominal 5 dorsal oblique muscle 4 rho5 abdominal 5 lateral longitudinal muscle 1 rho5 abdominal 5 lateral pentascolopidial chordotonal organ lch5 rho6 rhohs.PSt abdominal 5 ventral denticle belt rhounspecified abdominal 5 ventral longitudinal muscle 4 rho5 abdominal 5 ventral monoscolopidial chordotonal organ vch1 rho6 rhohs.PSt abdominal 5 ventral oblique muscle 1 rho5 abdominal 5 ventral oblique muscle 2 rho5 abdominal 6 dorsal acute muscle 1 rho7M43 abdominal 6 dorsal acute muscle 3 rho5 abdominal 6 dorsal oblique muscle 3 rho5 abdominal 6 dorsal oblique muscle 4 rho5 abdominal 6 lateral longitudinal muscle 1 rho5 abdominal 6 lateral pentascolopidial chordotonal organ lch5 rho6 rhohs.PSt abdominal 6 ventral longitudinal muscle 4 rho5 abdominal 6 ventral monoscolopidial chordotonal organ vch1 rho6 rhohs.PSt abdominal 6 ventral oblique muscle 1 rho5 abdominal 6 ventral oblique muscle 2 rho5 abdominal 7 dorsal acute muscle 1 rho7M43 abdominal 7 dorsal acute muscle 3 rho5 abdominal 7 dorsal oblique muscle 3 rho5 abdominal 7 dorsal oblique muscle 4 rho5 abdominal 7 lateral longitudinal muscle 1 rho5 abdominal 7 lateral pentascolopidial chordotonal organ lch5 rho6 rhohs.PSt abdominal 7 ventral longitudinal muscle 4 rho5 abdominal 7 ventral monoscolopidial chordotonal organ vch1 rho6 rhohs.PSt abdominal 7 ventral oblique muscle 1 rho5 abdominal 7 ventral oblique muscle 2 rho5 abdominal lateral pentascolopidial chordotonal organ lch5 rho7M43 rhoPΔ38 rhoPΔ5 abdominal lateral pentascolopidial chordotonal organ lch5 | supernumerary, with Scer\GAL4en-e16E rhoScer\UAS.cdCa adult eye primordium rhohs.PSt adult salivary gland primordium rho7M43 anal pad rho7M43 antenno-maxillary complex rho5 anterior commissure rho7M43 anterior crossvein, with Scer\GAL4e22c rhoScer\UAS.cUa axon rhounspecified Bolwig organ rho9 rhohs.PSt bract | somatic clone rho6 cephalopharyngeal skeleton rho7M43 rhoPΔ16 chordotonal organ rhoL68 rhohs.PSt chordotonal organ | precursor & embryonic abdomen rho5 chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4sca-109-68 rhoScer\UAS.cGa chorion, with Scer\GAL4CY2 rhoScer\UAS.cBa chorion, with Scer\GAL4T155 rhoScer\UAS.cGa chorion | ectopic | somatic clone, with Scer\GAL4Act5C.PP rhoScer\UAS.cGa circumesophageal tract | precursor rho7M43 commissure rho7M43 rhounspecified cone cell | ectopic, with Scer\GAL4GMR.PF rhoScer\UAS.cdCa crossvein | ectopic rhohs.PSt dendritic arborizing neuron rhounspecified denticle | ectopic, with Scer\GAL4en-e16E rhoScer\UAS.cGa denticle belt rho6 rho7M43 rhoPΔ16 rhodsRNA.cUa rhounspecified denticle belt, with Scer\GAL455B rhoScer\UAS.cGa denticle belt, with Scer\GAL4prd.RG1 rhoScer\UAS.cXa denticle belt | ventral rho6 rho7M43 denticle row 1 rhodsRNA.cUa denticle row 4 rhodsRNA.cUa dorsal appendage rhoa.hs rhohs.PSt dorsal appendage, with Scer\GAL4CY2 rhoScer\UAS.cBa dorsal appendage | cell non-autonomous | ectopic | somatic clone, with Scer\GAL4Act5C.PP rhoScer\UAS.cGa dorsal appendage | ectopic, with Scer\GAL455B rhoScer\UAS.cGa dorsal appendage | ectopic | somatic clone, with Scer\GAL4Act5C.PP rhoScer\UAS.cGa dorsal appendage | maternal effect | somatic clone rho6 rho7M43 dorsal hair, with Scer\GAL455B rhoScer\UAS.cGa dorsal mesothoracic disc primordium rho6 dorsal neurohemal organ rho6 rho7M43 dorsal neurohemal organ | ectopic, with Scer\GAL4prd.RG1 rhoScer\UAS.cXa egg rhoa.hs rhohs.PSt egg, with Scer\GAL4CY2 rhoScer\UAS.cdCa embryonic/first instar larval cuticle rhodsRNA.cUa rhounspecified embryonic/first instar larval cuticle, with Scer\GAL4en-e16E rhoScer\UAS.cGa embryonic/larval dorsal trunk rhoPΔ5 rhounspecified embryonic/larval oenocyte rho7M43 rhoPΔ5 embryonic/larval oenocyte | ectopic, with Scer\GAL4ato.3.6 rhoScer\UAS.cdCa embryonic/larval oenocyte | ectopic, with Scer\GAL4en-e16E rhoScer\UAS.cdCa embryonic/larval oenocyte | supernumerary, with Scer\GAL4en-e16E rhoScer\UAS.cdCa embryonic/larval oenocyte precursor | supernumerary, with Scer\GAL4en-e16E rhoScer\UAS.cdCa embryonic/larval optic lobe rhohs.PSt embryonic/larval optic lobe | posterior rho9 embryonic/larval trachea rhoPΔ38 embryonic/larval tracheal system rhoPΔ5 embryonic/larval visceral branch rhoPΔ5 embryonic anterior Malpighian tubule rho7M43 embryonic central brain rho7M43 embryonic central brain, with Scer\GAL4sim.PS rhoScer\UAS.cdCa embryonic epidermis rho5 rho6 embryonic head & external sensory organ rho5 embryonic Malpighian tubule rho7M43 embryonic mesothoracic segment, with Scer\GAL4ato.3.6 rhoScer\UAS.cdCa embryonic mesothoracic segment, with Scer\GAL4en-e16E rhoScer\UAS.cdCa embryonic metathoracic segment, with Scer\GAL4ato.3.6 rhoScer\UAS.cdCa embryonic metathoracic segment, with Scer\GAL4en-e16E rhoScer\UAS.cdCa embryonic neuroblast rho6 embryonic pericardial cell, with Scer\GAL4how-24B rhoEP3704 embryonic posterior Malpighian tubule rho7M43 embryonic prothoracic segment, with Scer\GAL4ato.3.6 rhoScer\UAS.cdCa embryonic prothoracic segment, with Scer\GAL4en-e16E rhoScer\UAS.cdCa embryonic thorax & external sensory organ rho5 embryonic trachea & cortical actin cytoskeleton rhoPΔ5 embryonic ventral epidermis rho7M43 embryonic ventral nervous system rho7M43 eye rhoGMR.Exel rhoScer\UAS.cBa rhohs.PSt rhohs.sev eye, with Scer\GAL4ey.PH rhoEP3704 eye, with Scer\GAL4GMR.PF rhoScer\UAS.cdCa eye, with Scer\GAL4hs.2sev rhoEP3704 eye photoreceptor cell | ectopic, with Scer\GAL4GMR.PF rhoScer\UAS.cdCa eye photoreceptor cell | somatic clone rhoPΔ5 ganglionic branch 1 | embryonic stage, with Scer\GAL4bs-23.26 rhoScer\UAS.cBa ganglionic branch 1 | embryonic stage, with Scer\GAL4sim.PS rhoScer\UAS.cBa genital disc primordium rho7M43 interommatidial bristle rhohs.sev Keilin's organ rho5 rho6 rho7M43 larval sense organ & antennal segment rho7M43 larval sense organ & maxillary segment rho7M43 lch5 cap attachment cell rhoPΔ38 lch5 ligament attachment cell rhoPΔ38 longitudinal connective rhoPΔ38 rhounspecified mesoderm rho7M43 mesoderm | ectopic, with Scer\GAL452B rhoScer\UAS.cXa midline glial cell rho5 rho6 rho7M43 rhoPΔ38 rhohs.PSt rhounspecified midline glial cell, with Scer\GAL4elav.PLu rhoScer\UAS.cGa midline glial cell | ectopic rhohs.PSt midline glial cell | ectopic, with Scer\GAL4sca-537.4 rhoScer\UAS.cXa motor neuron rhounspecified MP1 neuron rhoPΔ38 muscle founder cell | embryonic stage, with Scer\GAL4twi.PG rhoScer\UAS.cBa neuroblast rho6 rho7M43 oenocyte primordium, with Scer\GAL4en-e16E rhoScer\UAS.cdCa ommatidium rhoScer\UAS.cBa rhohs.sev optic lobe rhohs.PSt pale ommatidium | somatic clone rhoPΔ5 photoreceptor cell rhohs.PSt photoreceptor cell R8 rho7M43 pigment cell | ectopic, with Scer\GAL4GMR.PF rhoScer\UAS.cdCa posterior commissure rho7M43 posterior crossvein, with Scer\GAL4e22c rhoScer\UAS.cUa presumptive embryonic/larval central nervous system rhounspecified presumptive embryonic/larval peripheral nervous system rho5 rhounspecified presumptive embryonic/larval tracheal system rho7M43 presumptive embryonic/larval tracheal system, with Scer\GAL4salm-459.2 rhoScer\UAS.cdCa prothoracic ventral denticle belt rhounspecified RP2 neuron rho6 RP3 neuron rho5 scolopidial dendritic cap cell rho5 scolopidial neuron rho5 scolopidium rho5 segment border muscle, with Scer\GAL4how-24B rhoEP3704 segment border muscle | ectopic rhove-1 sensory neuron rhounspecified somatic muscle | dorsal rho5 tracheal branch primordium rhoPΔ5 tracheal primordium rho7M43 tracheal primordium, with Scer\GAL4salm-459.2 rhoScer\UAS.cdCa tracheal system rho207 ventral nerve cord commissure, with Scer\GAL4sca-4512 rhoEP3704 ventral thoracic disc primordium rho6 VUM1 neuron rhoPΔ38 VUM2 neuron rhoPΔ38 VUM3 neuron rhoPΔ38 VUM4 neuron rhoPΔ38 VUM5 neuron rhoPΔ38 VUM6 neuron rhoPΔ38 wing rhoMod rhoSld rhoStg rhohs.PSt rhoKG07115 wing, with Scer\GAL4Bx-MS1096 rhoΔN.Scer\UAS rhoNeo'.Scer\UAS rhoNeo.Scer\UAS rhoScer\UAS.cBa rhoScer\UAS.cUa rhoScer\UAS.cdCa wing | ectopic rhoStg wing disc | dorsal compartment | ectopic | somatic clone, with Scer\GAL4αTub84B.PZ rhoScer\UAS.cGa wing disc | dorsal compartment | somatic clone, with Scer\GAL4αTub84B.PZ rhoScer\UAS.cGa wing disc | somatic clone, with Scer\GAL4αTub84B.PZ rhoScer\UAS.cGa wing margin, with Scer\GAL4Bx-MS1096 rhoNeo'.Scer\UAS rhoNeo.Scer\UAS wing margin, with Scer\GAL4vg.int2.1 rhoNeo.Scer\UAS wing vein rhoMod rhoSld rhoStg rhoWk rhohs.PSt rhove-1 rhove-2 rhoKG07115 wing vein, with Scer\GAL4Bx-MS1096 rhoDN.Scer\UAS rhoNeo'.Scer\UAS rhoNeo.Scer\UAS rhoScer\UAS.cBa wing vein | distal rhove-1 wing vein | ectopic rhoMod rhoStg rhoWk rhohs.PSt wing vein | ectopic, with Scer\GAL4Bx-MS1096 rhoScer\UAS.cBa rhoScer\UAS.cdCa wing vein | ectopic | heat sensitive rhohs.PSt wing vein | germline clone rho6 wing vein | heat sensitive rhohs.PSt wing vein | supernumerary, with Scer\GAL4Bx-MS1096 rhoScer\UAS.cdCa wing vein L1 rhove-1 wing vein L2 rhove-1 wing vein L2 | germline clone rho6 wing vein L3 rhoMod rhohs.PSt rhove-1 wing vein L3, with Scer\GAL4e22c rhoScer\UAS.cUa wing vein L3 | distal rhove-1 wing vein L3 | germline clone rho6 wing vein L4 rhoMod rhove-1 rhove-3 wing vein L4, with Scer\GAL4e22c rhoScer\UAS.cUa wing vein L4 | germline clone rho6 wing vein L5 rhove-1 wing vein L6 rhove-1 yellow ommatidium rhoGMR.Exel yellow ommatidium, with Scer\GAL4GMR.PF rhoScer\UAS.cBa
Classical Alleles ( 31 )
For All Classical Alleles Show
Allele of rho Class Mutagen Stocks Known lesion rhove-1 loss of function allele spontaneous 45 Yes rhoEP3704 P-element activity 2 Yes rho7M43 amorphic allele - genetic evidence ethyl methanesulfonate 1 -- rhoAA69 P-element activity 1 Yes rhoBB02 1 Yes rhoe00892 piggyBac transposase 1 -- rhoKG07115 1 -- rhoL68 ethyl methanesulfonate 1 -- rho6 loss of function allele, amorphic allele - genetic evidence, hypomorphic allele - genetic evidence Delta2-3 0 Yes rhoPΔ38 amorphic allele - genetic evidence, loss of function allele Delta2-3 0 Yes rhoPΔ5 loss of function allele, amorphic allele - genetic evidence Delta2-3 0 Yes rho+ 0 -- rho10 ethyl methanesulfonate X ray 0 -- rho11 ethyl methanesulfonate X ray 0 -- rho207 ethyl methanesulfonate 0 -- rho5 loss of function allele, hypomorphic allele - genetic evidence P-element activity 0 Yes rho7 ethyl methanesulfonate X ray 0 -- rho8 ethyl methanesulfonate X ray 0 -- rho9 ethyl methanesulfonate X ray 0 -- rhodl11 0 -- rhoiks P-element activity 0 -- rhoMod gain of function allele P-element activity 0 Yes rhoPX62 0 Yes rhoPΔ16 Delta2-3 0 Yes rhoSld P-element activity 0 -- rhoStg gain of function allele P-element activity 0 Yes rhounspecified 0 Yes rhove-2 spontaneous 0 -- rhove-3 0 -- rhoWk gain of function allele P-element activity 0 Yes rhoX63 0 Yes
Alleles Carried on Transgenic Constructs ( 37 )
For All Alleles Carried on Transgenic Constructs Show
Allele of rho Class Mutagen Stocks Known lesion rhoGD2243 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes rhoGMR.Exel in vitro construct - regulatory fusion 1 Yes rhoJF03106 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes rhoKK111378 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes rhoScer\UAS.cdCa in vitro construct - regulatory fusion 1 Yes rhoa.hs in vitro construct - regulatory fusion in vitro construct 0 Yes rhoAct5C.PG in vitro construct - regulatory fusion 0 Yes rhoAct5C.T:Disc\RFP-DsRed2 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rhoDN.Scer\UAS Delta2-3 0 Yes rhodsRNA.cLa in vitro construct - RNAi 0 Yes rhodsRNA.cUa in vitro construct - RNAi 0 Yes rhodsRNA.Scer\UAS in vitro construct - RNAi 0 Yes rhoE1.hs in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes rhoE2.hs in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes rhoE3.hs in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes rhoE4.hs in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes rhohs.PSc in vitro construct - regulatory fusion 0 Yes rhohs.PSt in vitro construct - regulatory fusion 0 Yes rhohs.sev in vitro construct - regulatory fusion 0 -- rhoMtnA.PL in vitro construct - regulatory fusion 0 Yes rhoMtnA.PR in vitro construct - regulatory fusion 0 Yes rhoMtnA.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rhoN.Scer\UAS in vitro construct - regulatory fusion 0 Yes rhoNeo'.Scer\UAS in vitro construct - regulatory fusion 0 Yes rhoNeo.Scer\UAS Delta2-3 0 Yes rhoRyn in vitro construct - genomic fragment 0 Yes rhoScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes rhoScer\UAS.cGa in vitro construct - regulatory fusion 0 Yes rhoScer\UAS.cTa in vitro construct - regulatory fusion 0 Yes rhoScer\UAS.cUa in vitro construct - regulatory fusion 0 Yes rhoScer\UAS.cXa in vitro construct - regulatory fusion 0 Yes rhoScer\UAS.P\T.cGa in vitro construct - regulatory fusion 0 Yes rhoScer\UAS.T.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rhoScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rhoScer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rhoScer\UAS.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rhoΔN.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Duplicated in	Dp(1;YS)scV1mwh+   Dp(3;Y;1)M2 (Roch et al., 1998) T(Y;3)P6
Disrupted in	Df(3L)Ar14-8 (Wasserman et al., 2000) Df(3L)H141 (Jurgens et al., 1984, Tearle and Nusslein-Volhard, 1987) Df(3L)ru-22 (Wasserman et al., 2000) Dp(3;Y;1)M2 (Garcia-Bellido et al., 1994) Ts(2Rt;3Rt)Dll15 (Jurgens et al., 1984) Ts(Y;3Rt)G130 (Jurgens et al., 1984)
Not disrupted in	Ts(YSt;3Rt)A114 (Tearle and Nusslein-Volhard, 1987)
Transgenic Constructs & Insertions
Transgenic Constructs
reporter construct	Name Expression Data P{2x-55kni-lacZ-rhoNEE700} No P{2x-130kni-lacZ-rhoNEE700} No P{2XPE-Kr-rhoNEE} No P{2XPE-rhoNEE} No P{2XPE-rhoNEE-Kr} No P{3NEE5-stripe2-lacZ} No P{5NEE3-stripe2-lacZ} No P{50/50-lacZ} No P{150/120-lacZ} No P{E3-SU} No P{eve-CAT.eve/w-lacZ.NEE.IAB5} No P{eve-CAT.w/TATA-lacZ.NEE.IAB5} No P{NEE-2xPE-lacZ} No P{NEE-50-lacZ} No P{NEE-50Δs-lacZ} No P{NEE-150-lacZ} No P{NEE.PE-lacZ} No P{NEEdbs-lacZ} No P{NEEds-lacZ} No P{NEE-GFP-2xh50-lacZ} No P{NEE-GFP-h50-lacZ} No P{NEE-h-2xPE-lacZ} No P{NEE-h50-lacZ} No P{NEE-h150-lacZ} No P{NEE-h250-lacZ} No P{NEE-h-Ance-lacZ} No P{NEE-h-CAT-2xPE-lacZ} No P{NEE-h-CAT-GFP-2xPE-lacZ} No P{NEE-Kr50-lacZ} No P{NEEs2-lacZ} No P{NEEsna.AB} No P{NEEsna.CD} No P{NEE-SU-H1} No P{NEEΔs-lacZ} No P{NEEΔsna.AB} No P{NEEΔsna.CD} No P{NEE-λ1.4-H1} No P{rho330-2xtwiPE-2xknirps70-lacZ} No P{rho330-2xtwiPE-2xknirps75-lacZ} No P{rho330-2xtwiPE-2xknirps100-lacZ} No P{rho330-2xtwiPE-2xknirps130-lacZ} No P{rho330-2xtwiPE-2xknirps180-lacZ} No P{rho330-2xtwiPE-2xknirps-lacZ} No P{rho.1-lacZ} No P{rho.2-lacZ} No P{rho.3-lacZ} No P{rho.4-lacZ} No P{rho.5-lacZ} No P{rho.6-lacZ} No P{rho.7-lacZ} No P{rho.8-lacZ} No P{rho.NEE-lacZ} No P{Rho(ve)-lacZ.0.2} No P{Rho(ve)-lacZ.0.4} No P{Rho(ve)-lacZ.0.7} No P{Rho(ve)-lacZ.1.2} No P{Rho(ve)-lacZ.1.6} No P{Rho(ve)-lacZ.1.8} No P{Rho(ve)-lacZ.2.0} No P{Rho(ve)-lacZ.2.2} No P{Rho(ve)-lacZ.EE} No P{Rho(ve)-lacZ.ESs} No P{Rho(ve)-lacZ.HA} No P{Rho(ve)-lacZ.SX} No P{rho-BR-h-lacZ.NSSH} No P{snaNEE.50} No P{snaNEE.125} No P{UAS-NEE-lacZ} No P{veNEE-lacZ} No P{w-w.w-CAT.eve-lacZ.NEE.IAB5} No
UAS construct	Name Expression Data P{GD2243} NA P{KK111378} NA P{TRiP.JF03106} NA P{UASp-rho.G} NA P{UAS-rho1.T} NA P{UAS-Rho.DN} NA P{UAS-rho.FLAG} NA P{UAS-rho.HA} NA P{UAS-rho.N} NA P{UAS-Rho.Neo'} NA P{UAS-rho.T.HA} NA P{UAS-rho.TAP} NA P{UAS-rho.U} NA P{UAS-rho.ΔN} NA P{UAS-rho-dsRNA} NA P{UAS-ve.(rho)G} NA P{UAS-ve.(rho)X} NA P{UAS-ve.B} NA P{UAS-ve.dC} NA
heat-shock construct	Name Expression Data P{E1} NA P{E2} NA P{E3} NA P{E4} NA P{hs-rho.a} NA P{HS-rho} NA P{vehs.sev} NA
characterization construct	Name Expression Data P{GMR-rho.Exel} NA P{NEE-3x.SF-1b1-H1} NA P{NEE-3x.SF-1b2-H1} NA P{NEE-3x.SF-1b3-H1} NA P{NEE-3x.SF-1b3-KO-H1} NA P{NEE-SF-1a-H1} NA P{NEE-SF-1b1-H1} NA P{NEE-SF-1b2-H1} NA P{NEE-SF-1b3-H1} NA P{NEE-SF-1b-H1} NA P{NEE-SF-1c-H1} NA P{NEE-SF-1-H1} NA P{NEE-SF-1rev-H1} NA P{rho.8.3.Ryn} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}rhoEP3704 NA insertion of enhancer trap P{A92}rhoAA69 No P{A92}rhoBB02 No P{lacW}l(3)SH019SH019 No P{lacW}rho5 No P{lacZ}rhoX63 No P{lwB}X81 No miscellaneous insertions P{3'lacW}rho6 NA P{HS-rho}Mod NA P{HS-rho}Sld NA P{HS-rho}Stg NA P{HS-rho}Wk NA P{PTT-GB}CB05698 NA P{SUPor-P}rhoKG07115 NA PBac{RB}rhoe00892 NA insertion of enhancer trap binary system P{GAL4}rhoiks No P{GawB}rhl No
Gene Ontology: Function, Process & Cellular Component ( 34 unique terms )
Terms Based on Experimental Evidence ( 20 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase activity inferred from direct assay (Urban et al., 2001) inferred from genetic interaction with spi (Urban et al., 2001) inferred from mutant phenotype (Urban et al., 2001)
Biological Process
CV term Evidence References
axonogenesis inferred from mutant phenotype (Grueber et al., 2007) behavioral response to ethanol inferred from mutant phenotype (Corl et al., 2009) brain development inferred from mutant phenotype (Page, 2003) cell-cell adhesion inferred from mutant phenotype (Cela and Llimargas, 2006) cytokinesis inferred from mutant phenotype (Rogers et al., 2003) determination of genital disc primordium inferred from mutant phenotype (Chen et al., 2005) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Dworkin and Gibson, 2006) imaginal disc-derived wing vein morphogenesis inferred from mutant phenotype (Ralston and Blair, 2005, Marenda et al., 2006, Ragab et al., 2006) imaginal disc-derived wing vein specification inferred from mutant phenotype (Marenda et al., 2006, Brentrup et al., 2000) learning or memory inferred from mutant phenotype (Dubnau et al., 2003) maintenance of epithelial integrity, open tracheal system inferred from mutant phenotype (Cela and Llimargas, 2006) negative regulation of gene expression inferred from mutant phenotype (Brentrup et al., 2000) oenocyte development inferred from genetic interaction with abd-A AND inferred from mutant phenotype (Brodu et al., 2002) olfactory learning inferred from mutant phenotype (Dubnau et al., 2003) peptide bond cleavage involved in epidermal growth factor receptor ligand maturation inferred from genetic interaction with spi (Lee et al., 2001, Urban et al., 2001) wing cell fate specification inferred from mutant phenotype (Marenda et al., 2006)
Cellular Component
CV term Evidence References
NOT endoplasmic reticulum inferred from direct assay (Lee et al., 2001) Golgi apparatus inferred from direct assay (Lee et al., 2001, Urban et al., 2001) NOT plasma membrane inferred from direct assay (Lee et al., 2001)
Terms Based on Predictions or Assertions ( 17 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase activity inferred from sequence model (Urban et al., 2001)
Biological Process
CV term Evidence References
branched duct epithelial cell fate determination, open tracheal system traceable author statement (Zelzer and Shilo, 2000) epidermal growth factor receptor signaling pathway traceable author statement (Bier, 1998) imaginal disc-derived wing vein specification non-traceable author statement (de Celis, 2003) traceable author statement (Fristrom et al., 1994) leg disc proximal/distal pattern formation traceable author statement (Mann and Casares, 2002) morphogenesis of a branching structure non-traceable author statement (Affolter and Shilo, 2000) open tracheal system development traceable author statement (Affolter and Shilo, 2000, Bradley et al., 2001, Uv et al., 2003, Ghabrial et al., 2003, Nelson, 2003) ovarian follicle cell development traceable author statement (Dobens and Raftery, 2000) peripheral nervous system development traceable author statement (Salzberg et al., 1994) positive regulation of epidermal growth factor receptor signaling pathway traceable author statement (van Eeden and St. Johnston, 1999) proteolysis inferred from electronic annotation with InterPro:IPR002610 (FlyBase Curators et al., 2004-) salivary gland boundary specification non-traceable author statement (Bradley et al., 2001) salivary gland development traceable author statement (Abrams et al., 2003) tracheal pit formation in open tracheal system traceable author statement (Uv et al., 2003, Ghabrial et al., 2003)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR002610, InterPro:IPR017213, InterPro:IPR022764 (FlyBase Curators et al., 2004-) inferred from sequence model (Urban et al., 2001) traceable author statement (Bier, 1998) integral to plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1991.2.1) plasma membrane inferred from sequence or structural similarity (Bier et al., 1990)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions rho allele Gene References rho5 H (Johannes and Preiss, 2002) rho7M43 ru (Wasserman et al., 2000, Yang and Baker, 2003) Src42A (Zhang et al., 1999) tum (Sotillos and Campuzano, 2000) rho9 bs (Roch et al., 1998) shg (Dumstrei et al., 2002) vn (Roch et al., 1998) rho10 arm (Greaves et al., 1999) rhodsRNA.cUa ru (Urban et al., 2004) rhohs.PSt bs (Sturtevant and Bier, 1995, Roch et al., 1998) dpp (de Celis, 1997) ed (Bai et al., 2001) S (Guichard et al., 1999) spi (Wasserman and Freeman, 1998, Guichard et al., 1999, Lanoue and Jacobs, 1999) tkv (de Celis, 1997) vap (Botella et al., 2003) rhohs.sev aop (Freeman, 1994) Egfr (Freeman, 1994) Ras85D (Freeman, 1994) S (Freeman, 1994) Sos (Freeman, 1994) spi (Freeman, 1994) sty (Casci et al., 1999) rhoMod sog (Yu et al., 1996) rhoScer\UAS.cBa Egfr (Buff et al., 1998, Guichard et al., 1999) kni (Lunde et al., 2003) ru (Gallio et al., 2004) S (Guichard et al., 1999) stet (Schulz et al., 2002) rhoScer\UAS.cdCa ato (Brodu et al., 2002) ft (Garoia et al., 2005) rhoScer\UAS.cGa vn (Zecca and Struhl, 2002) rhoScer\UAS.cUa net (Brentrup et al., 2000) rhoStg dpp (Yu et al., 1996) emc (Baonza and Garcia-Bellido, 1999) msk (Baker et al., 2002) sog (Yu et al., 1996) rhounspecified Egfr (Diaz-Benjumea and Garcia-Bellido, 1990) vn (Diaz-Benjumea and Garcia-Bellido, 1990, Urban et al., 2004) rhove-1 aos (Sawamoto et al., 1994) brm (Marenda et al., 2004) bs (Sturtevant and Bier, 1995, Roch et al., 1998) Egfr (Guichard et al., 1999) H (Johannes and Preiss, 2002) Pc (Marenda et al., 2004) px (Wessendorf et al., 1992) Snr1 (Marenda et al., 2004) tkv (de Celis, 1997) vn (de Celis, 1997, Roch et al., 1998, Ralston and Blair, 2005) rhoWk emc (Baonza and Garcia-Bellido, 1999) msk (Baker et al., 2002) S (Guichard et al., 1999) unspecified ab (Sturtevant and Bier, 1995) ac (Sturtevant and Bier, 1995) blo (Sturtevant and Bier, 1995) bs (Fristrom et al., 1994, Montagne et al., 1996) Bx (Sturtevant and Bier, 1995) cg (Sturtevant and Bier, 1995) chl (Sturtevant and Bier, 1995) ci (Sturtevant and Bier, 1995) cp (Sturtevant and Bier, 1995) cv-c (Sturtevant and Bier, 1995) da (Sturtevant and Bier, 1995) Dl (Sturtevant and Bier, 1995) dp (Sturtevant and Bier, 1995) dpp (Sturtevant and Bier, 1995) ds (Sturtevant and Bier, 1995) dsr (Sturtevant and Bier, 1995) dx (Sturtevant and Bier, 1995) Dys (Sturtevant and Bier, 1995) E(spl) (Sturtevant and Bier, 1995) emc (Sturtevant and Bier, 1995) en (Sturtevant and Bier, 1995) fj (Sturtevant and Bier, 1995) ft (Sturtevant and Bier, 1995) fu (Sturtevant and Bier, 1995) grk (Sapir et al., 1998) Gug (Charroux et al., 2006) H (Sturtevant and Bier, 1995, Mueller et al., 2005) h (Sturtevant and Bier, 1995) hh (Sturtevant and Bier, 1995) HmgD (Ragab et al., 2006) HmgZ (Ragab et al., 2006) if (Sturtevant and Bier, 1995) kn (Sturtevant and Bier, 1995) kni (Sturtevant and Bier, 1995) mam (Sturtevant and Bier, 1995) mys (Sturtevant and Bier, 1995) N (Sturtevant and Bier, 1995) nej (Anderson et al., 2005) net (Sturtevant and Bier, 1995) neur (Sturtevant and Bier, 1995) NX (Sturtevant and Bier, 1995) ny (Sturtevant and Bier, 1995) p53 (Mueller et al., 2005) ptc (Sturtevant and Bier, 1995) px (Sturtevant and Bier, 1995) r (Sturtevant and Bier, 1995) S (Sapir et al., 1998) sc (Sturtevant and Bier, 1995) sd (Sturtevant and Bier, 1995) sens (Sturtevant and Bier, 1995) Ser (Sturtevant and Bier, 1995, Walters et al., 2005) shf (Sturtevant and Bier, 1995) std (Sturtevant and Bier, 1995) tg (Sturtevant and Bier, 1995) th (Sturtevant and Bier, 1995) tk (Sturtevant and Bier, 1995) tkv (Sturtevant and Bier, 1995) tt (Sturtevant and Bier, 1995) vn (Sturtevant and Bier, 1995) Vno (Sturtevant and Bier, 1995) vs (Sturtevant and Bier, 1995) vvl (Sturtevant and Bier, 1995, de Celis et al., 1995) W (Mueller et al., 2005) wg (Sturtevant and Bier, 1995)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63708 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004635 DroID - A comprehensive database of gene and protein interactions. • FBgn0004635 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38168
Orthologs
Genome-wide drosophilid orthologs	Dana\rho Dere\GG14792 Dgri\GH16240 Dmoj\GI16668 Dper\GL22508 Dpse\rho Dsec\ve Dsim\GD13619 Dvir\rho Dwil\rho Dyak\GE21154
Curated drosophilid orthologs	Dafs\ve (Sturtevant and Novitski, 1941) Dana\rho (Zinzen et al., 2006) Dmau\ve   Dmoj\rho (Zinzen et al., 2006) Dpse\rho   Dpse\sh (Sturtevant and Novitski, 1941, Sturtevant and Tan, 1937) Dsec\ve   Dsim\ve   Dvir\rho (Nakamura and Matsuno, 2002) Dvir\sv (Sturtevant and Novitski, 1941) Dwil\sh (Sturtevant and Novitski, 1941, Spassky and Dobzhansky, 1950) Dwil\ve (Spassky and Dobzhansky, 1950) Dyak\rho (Zinzen et al., 2006)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • KLLA0D15224g Kluyveromyces lactis (yeast) LOC_Os04g48130.1 Oryza sativa (asian rice) LOC_Os08g43320.1 Oryza sativa (asian rice) 228199 Physcomitrella patens (moss) 824920 Populus trichocarpa (black cottonwood) PVX_088955 Plasmodium vivax (protozoal parasite) PVX_091350 Plasmodium vivax (protozoal parasite) 4776593 Sorghum bicolor (sorghum) 4891440 Sorghum bicolor (sorghum) 85781.m00157 Trichomonas vaginalis (parasitic flagellated protozoan) 89678.m00174 Trichomonas vaginalis (parasitic flagellated protozoan) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • ISCW021997 FBgn0238430 FBgn0218983 FBgn0062278 FBgn0185329 FBgn0025045 FBgn0070086 FBgn0160100 FBgn0139416 FBgn0123711 FBgn0107048 FBgn0101759
Stocks & Reagents
Stocks Listed in FlyBase ( 59 )
Bloomington	628 rhove-1 2399 Df(3L)R-G5, rhove-1/TM6 1029 rhove-1 Coa1/TM6 5800 P{A92}rhoAA69 ry506
Harvard	- PBac{RB}rhoe00892
Kyoto	106017 rhove-1 107310 Df(3L)R-G5, rhove-1/TM6 101596 Df(3L)R-G5, rhove-1 / TM6 106176 rhove-1 Coa1/TM6
VDRC	v107502 P{KK111378}VIE-260B v51952 w1118; P{GD2243}v51952/TM3 v51953 w1118; P{GD2243}v51953/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR27O01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 37 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD06131
Other clones	DPiM_LD06131
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP22742 RE59529 RH09903 RH10122 RH45513 RH58702
Other clones	30036 45539 179697 572885 CK01.20B.H3 EC052343 EC053735 EC054759 EC054858 EC055330 EK018823 EK030503 EK161553 EK222204 EN17311 RM05212 RM05512 RM05612 RM06212 RP001000416 RP001015072 RP001019795 RP001020147 RP001020750 RP001023178 RP001031038 RP001051762 RP001052875 RP001055841
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004635 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38168
Antibody Information
	monoclonal (Freeman et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: rho CG1004 (Lei, 1999.11)
Source for database merge of
Additional comments
Other Comments
	rho is not required for patterning of the dorsal anterior follicular epithelium. (Lachance et al., 2009) rho protein cleaves S within its transmembrane domain. (Tsruya et al., 2007) msk is involved in the nuclear transportation of rho. (Marenda et al., 2006) rho, sim and vn are required for the formation of the brain lateral to the foregut cells. (Page, 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) rho protein is localised in the Golgi, where it promotes the cleavage of spi protein. (Lee et al., 2001) rho protein appears to be an intramembrane serine protease that directly cleaves spi protein. The putative rho active site is within the membrane bilayer and the spi cleavage site is within its transmembrane domain. (Urban et al., 2001) net exhibits a two tiered control in wing vein versus intervein fate by repressing rho transcription and interfering with Egfr signalling downstream of rho. (Brentrup et al., 2000) rho and S function in a synergistic and co-dependent manner, that is independent of spi, to promote vein development through the Egfr/MAPK signalling pathway. (Guichard et al., 1999) Five EMS induced alleles were identified in a screen for mutations affecting commissure formation in the CNS of the embryo. (Hummel et al., 1999) rho is required continuously fromm embryonic stage 9-17 to suppress apoptosis in the midline glial (MG) cells. rho may enhance autocrine and paracrine signalling among MG cells. (Lanoue and Jacobs, 1999) Candidate gene for quantitative trait (QTL) locus determining bristle number. (Nuzhdin et al., 1999) The rho NEE enhancer element does not discriminate between TATA-containing and TATA-less promoters. (Ohtsuki et al., 1998) The differentiation of vein and intervein depends on the outcome of a balance between bs and rho activities, achieved during pupal stages. (Roch et al., 1998) Immediately after the movement of the oocyte nucleus to the future dorsal pole a broad activation of the Egfr pathway takes place. As a result, all follicle cells, except the ventral-most rows, express Egfr-target genes. After completion of cell migration, transcription of rho in the dorsal-anterior follicle cells is achieved by activation of the Egfr pathway, in conjunction with signals that may emanate from the anterior, stretch follicle cells. Ectopic activation of rho in the stretch follicle cells can lead to activation of the Egfr pathway in the follicle cells covering the oocyte. Results suggest that rho is responsible for triggering the production or processing of a Egfr ligand that is expressed in the follicle cells. (Sapir et al., 1998) rho mediates autocrine spi signalling in the follicle cells. (Wasserman and Freeman, 1998) Genetic combinations with mutants of nub cause additive phenotypes. (Cifuentes and Garcia-Bellido, 1997) Study of expression and function of different components of the N pathway in both the wing disc and pupal wings proposes that the establishment of vein thickness utilises a combination of mechanisms. A mechanisms includes repression of rho transcription by HLHmβ and maintenance of Dl expression by rho/Egfr activity. (de Celis et al., 1997) vvl is required for the specific expression in the tracheal cells of tkv and rho. (Llimargas and Casanova, 1997) In vivo culture of mutant discs from genotypes that are normally embryonic lethal demonstrates rho has no role in wing disc growth. (Simcox, 1997) Clonal analysis suggests salm determines the position of the L2 vein primordium by activating rho expression in neighbouring cells through a locally non-autonomous mechanism. rho then functions to initiate and maintain vein differentiation. (Sturtevant et al., 1997) Analysis of rho mutants and targetted rho expression demonstrates the EGF signaling pathway regulates the number of both the dorsal median cells, as well as a set of mesodermal cells that arise next to the midline and express sim. (Zhou et al., 1997) The function of spi, rho and S appears to be non-autonomous; expression of the precursor only in the midline is sufficient for patterning the ventral ectoderm. Facilitating the expression of spi, rho and S is the only sim-dependent contribution of the midline to patterning the ventral ectoderm, since the mutant sim ectodermal defects can be overcome by expression of secreted spi in the ectoderm. These results suggest a mechanism for generating a graded distribution of secreted spi, which may subsequently give rise to graded activation of Egfr in the ectoderm. (Golembo et al., 1996) Loss of function mutations in bs are epistatic to loss of function mutations in rho or vn. (Montagne et al., 1996) The phenotype of a argos null mutant is not observed in a rho mutant background, indicating that rho acts epistatically to argos to regulate the correct number of Ch organs in the embryonic PNS. (Okabe et al., 1996) The rho protein is concentrated in patches at the apical cell surface. It is possible that rho plaques represent specialized structures defining sites of cell-cell contact at which Egfr signalling is particularly effective. (Sturtevant et al., 1996) rho is not required for early expression of sim or vnd in mesectodermal or ventral ectodermal cells, targeted rho expression in embryos results in lateral-to-ventral cell fate shifts in the developing neuroectoderm and midline targeted rho expression can rescue the medial denticle fusion in rho mutant cuticles. (Xiao et al., 1996) Mutations of rho enhance penetrance of ectopic crossveins in chicgdh-5/EgfrE1 heterozygotes. (Doberstein et al., 1995) Molecular analysis suggests that sna protein acts over distances of 50-150bp to block the activity, but not the binding, of the dl activator to the rho 650bp enhancer. (Gray et al., 1995) The spi product triggers the Egfr signaling cascade. Graded activation of the Egfr pathway may normally give rise to a repertoire of discrete cell fates in the ventral ectoderm and graded distribution of spi may be responsible for the graded activation. The rho and S products may act as modulators of Egfr signaling. Epistatic relationships suggest that rho and S may normally facilitate processing of the spi precursor. (Schweitzer et al., 1995) Ectopic expression of both rho and Dl in a mutant net background produces ectopic veins of normal thickness. Ectopic expression of rho alone produces whole intervein sectors converted into vein. The pattern of normal+ectopic wing veins resembles wing vein patterns of other flies with more veins than Drosophila. (Sturtevant et al., 1995) Double mutant genetic clones with vn have extreme nonautonomous effects in the proliferation of wild type cells in the wing. (Garcia-Bellido et al., 1994) Dorsal-ventral patterning is regulated by a signalling pathway that includes Tl and transcription factors, dl, that interact with related enhancers, rho. The κ enhancer from mouse is capable of generating lateral stripes of Ecol\lacZ gene expression in transgenic embryos in a pattern similar to that directed by rho enhancer. Results suggest that enhancers can couple conserved signalling pathways to divergent gene functions, dorso-ventral patterning and mammalian haematopoiesis. (Gonzalez-Crespo and Levine, 1994) A single sna-binding site in the rho promoter region can mediate repression of rho. (Gray et al., 1994) Enhancer piracy lines reveal an unanticipated role for rho in imaginal disc formation and provide evidence that mis-expression of rho is sufficient for converting entire intervein sectors into veins. (Noll et al., 1994) Mutation in rho affects sensory organ precursor formation. (Salzberg et al., 1994) rho is required for PNS development in the embryo. (Salzberg et al., 1994) The differentiation of individual mesectoderm cells (MECs) lineages is traced. rho is necessary for determining the correct number of cells in many neuronal MEC lineages. The correct number of midline glia are determined but later become apoptotic in embryos mutant for rho. (Sonnenfeld and Jacob, 1994) rho is involved in the downregulation of Egfr mRNA. (Sturtevant et al., 1994) Analysis of mutant embryos determines that growth cones can distinguish between individual muscle fibres during synaptogenesis. Growth cones retain their target preference even when the numbers and patterns of muscle fibres are altered. (Chiba et al., 1993) The E boxes within the neural ectoderm expression enhancer region (NEE) play a role in neuroectoderm gene expression. (Gonzalez-Crespo and Levine, 1993) Promoter fusions using elements of the twi, ve, da and sna promoters indicate that low affinity dl-binding sites restrict target gene expression to the presumptive mesoderm, where there are peak levels of dl expression, while high affinity sites in other target genes permit expression in ventrolateral regions where dl levels are intermediate. Activation by low levels of dl in lateral regions depends on cooperative interaction between dl and other basic helix loop helix proteins. Promoters containing the Et (veinlet) or Eds (dl and snail) E boxes display opposite behaviour in da and twi mutants, suggesting they are regulated by different basic helix loop helix proteins. (Jiang and Levine, 1993) The gene product is localized on the apical surface of the dorsal-anterior follicle cells surrounding the oocyte. Loss of function mutations cause ventralization of the egg shell and embryo, whereas ectopic expression leads to their dorsalization. Double mutant analysis indicates that rho acts upstream of Toll in dorsal-ventral axis formation, and the action of rho requires the grk-Egfr signaling pathway. rho expression pattern in embryogenesis is altered in fs(1)K10 mutants. (Ruohola-Baker et al., 1993) A H{Lw2} insertion at cytological location 62A (line 79) is viable and causes a recessive rough eye phenotype. The H{Lw2} element may have inserted into a gene adjacent to rho causing the rough eye phenotype and is responding to rho enhancer elements or the insertion may be into the rho gene causing an undescribed eye phenotype. (Smith et al., 1993) rho is likely to be the earliest known gene to be expressed in the longitudinal wing vein primordia and the rho continues to be expressed in developing wing veins during the partitioning of the wing into vein versus intervein areas. (Sturtevant et al., 1993) Role of rho in eye development studied: whereas mutant clones in the eye have only a subtle phenotype, ectopic expression of rho causes non-neural mystery cells to be transformed into photoreceptors. (Freeman et al., 1992) Expression of rho is blocked in ventral regions by sna. A neural ectoderm expression region (NEE) of 300bp has been defined in the rho promoter, and contains a cluster of dl and basic HLH activator sites closely linked to a sna repressor sites. Mutations in these sites cause predicted changes in the level of expression. Similarity of this system to eve stripe 2 suggests dl and bcd use similar mechanisms to generate their respective stripes. (Ip et al., 1992) rho gene product is required for the proper development of the ventralmost cuticle and the CNS midline. (Panzer et al., 1992) Basic protein structure, comparison of phenotypes and spatial and temporal expression patterns suggest that spi encodes a ligand that functionally interacts with the products of rho and possibly Egfr. (Rutledge et al., 1992) Zygotically active locus involved in the terminal developmental program in the embryo. (Strecker et al., 1991) Mutations lead to ventrolateral pattern defects and peripheral nervous system abnormalities. (Bier et al., 1990) rho is involved in the elaboration of positional information at a ventrolateral level in the embryo. rho acts very early in differentiation pathways to specify the identities of domains and isolated precursor cells. rho, pnt, S and spi all function in the formation of the same chordotonal organs. (Bier et al., 1990) Mutations in rho cause pleiotropic phenotypes in embryonic patterns and affect several longitudinal veins. (Diaz-Benjumea and Garcia-Bellido, 1990) sim gene product is required for the normal expression of rho. (Nambu et al., 1990, Nambu et al., 1991) ve, vn, ci, cg, svs, ast, H, Vno and vvl belong to the vein phenotypic group (Puro, 1982, Droso. Info. Serv. 58:205--208) within the 'lack-of-vein' mutant class. Loss-of-function alleles at these loci remove stretches of veins in two or more longitudinal veins. Double mutations within members of this group remove all veins, have smaller, slightly lanceolate wings, no sensilla and extra chaetae. Some alleles are embryonic lethal. (Diaz-Benjumea et al., 1989) rho mutants display a pointed head skeleton and deletion of the medial portion in all denticle belts. (Jurgens et al., 1984) Viable alleles exhibit wing venation defects; strong alleles are embryonic lethal. In flies homozygous for viable alleles the L3, L4 and L5 veins do not reach the wing margins (Duncan, 1935; Waddington, 1939). Developmentally, veins appear complete in prepupa but distal tips are obliterated during the contraction period (Waddington, 1939; Waddington, 1940). The shortened-vein phenotype is suppressed by px1 (Waddington), net1, and su(ve)1 and is enhanced by vn1, H1, NAx-1, ci1, tg2 and kniri-1 (Waddington; Diaz-Benjumea and Garcia-Bellido, 1990). Vein-specific modifiers, such as gp1, (Bridges and Morgan, 1919) or PL(2)L4 (Thompson, 1976), interact with the effect of rhove-1 on L4. The L5 vein seldom extends beyond the posterior crossvein. rhove-2 is a stronger allele, in which the L2 is also affected (Bertschmann); L2 vein occasionally complete (Thompson, 1976), but other veins do not overlap wild type. Distribution of sense organs (campaniform sensilla and bristles) on L3 is shifted proximally in rhove-1 (Spivey and Thompson, 1984) When a rhove-1 stock is selected for shortened veins, the F1 produced by mating wild-type males to mutant females show terminal gaps in L5 (Thompson and Thoday, 1976). rhove-1/rhove-1/+ intersexes are veinlet, whereas rhove-1/rhove-1/+ triploids are normal, according to Pipkin. Interestingly flies heterozygous for rhove-1 and strong embryonic lethal alleles display less severe veinlet phenotypes than rhove-1 homozygotes (Bier, Jan and Jan, 1990; Diaz-Benjumea and Garcia-Bellido, 1990); furthermore, rhove-1/rho5 flies appear wild type (Bier, unpublished). Homozygous rho5 embryos exhibit three major types of defects: (1) Dorsoventral defects: Embryos exhibit a deletion of epithelial cells derived from a ventrolateral strip of the blastoderm fate map (i.e., loss of mediolateral cuticular denticles and sensory structures). Other phenotypes resulting from blastoderm patterning defects include failure to complete dorsal closure and development of an abnormal pointed head skeleton (Jurgens et al., 1984; Mayer and Nusslein-Volhard, 1988). (2) Midline defects: Mesectodermal cells giving rise to glia and unpaired neurons are abnormal or fail to form. Late developmental consequences include a narrower CNS and pathfinding abnormalities (Mayer and Nusslein-Volhard, 1988). (3) Peripheral-nervous-system defects: Two stretch receptor organs (lateral abdominal chordotonal organs) fail to form in lethal rhove-1 mutants. The primary chordotonal-organ-precursor cells are likely to be affected since the four progeny sensory-organ cells derived from that precursor cell are missing as a group (Bier, Jan and Jan, 1990). Other late embryonic defects include loss of longitudinal body-wall muscles, ventrally displaced muscle-attachment sites (Bier, Jan and Jan, 1990) and loss of the first row of denticles in abdominal segments (Mayer and Nusslein-Volhard, 1988).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079159 RefSeq (Proteins) • NP_523883 DDBJ / EMBL / GenBank • AA247028 AAF47496 AAM51968 AF067877 AF067878 AW941611 AY121641 CAA36692 CZ478728 X52454 Entrez Gene - A searchable database of RefSeq genes. • 38168 UniProtKB/Swiss-Prot • P20350 UniProtKB/TrEMBL • Q540V7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S54, rhomboid (IPR002610) Peptidase S54, rhomboid, metazoan (IPR017213) Peptidase S54, rhomboid domain (IPR022764)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63708 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004635 DroID - A comprehensive database of gene and protein interactions. • FBgn0004635 DRSC - Results from RNAi screens. • FBgn0004635 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004635 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1004-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004635 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38168 InParanoid A subset of ortholog calls from InParanoid. • KLLA0D15224g Kluyveromyces lactis (yeast) LOC_Os04g48130.1 Oryza sativa (asian rice) LOC_Os08g43320.1 Oryza sativa (asian rice) 228199 Physcomitrella patens (moss) 824920 Populus trichocarpa (black cottonwood) PVX_088955 Plasmodium vivax (protozoal parasite) PVX_091350 Plasmodium vivax (protozoal parasite) 4776593 Sorghum bicolor (sorghum) 4891440 Sorghum bicolor (sorghum) 85781.m00157 Trichomonas vaginalis (parasitic flagellated protozoan) 89678.m00174 Trichomonas vaginalis (parasitic flagellated protozoan) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /torstoll/rhombid1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38168 modMine - Data generated by the modENCODE project. • FBgn0004635 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • ISCW021997 FBgn0238430 FBgn0218983 FBgn0062278 FBgn0185329 FBgn0025045 FBgn0070086 FBgn0160100 FBgn0139416 FBgn0123711 FBgn0107048 FBgn0101759 REDfly - A database of transcriptional regulatory elements. • FBgn0004635
Synonyms & Secondary IDs ( 27 )
Reported As
Symbol Synonym	CG1004 (Klebes et al., 2005, Dubnau et al., 2003, Keleman et al., 2009.8.5, Perkins et al., 2009, Muse et al., 2007) Dmel\rho (Schaeffer et al., 2008) DMRHO (Colson and Goldstein, 1999) Dmrho (Crocker et al., 2008) DMRHOa (Schug et al., 1997) DMRHOb (Rand et al., 2000, Schug et al., 1997) DRORHO (Schug et al., 1998) rho (McNeill et al., 2008, Jordan et al., 2005, Dworkin and Gibson., 2006, Kim et al., 2007, Chanut-Delalande et al., 2006, Reeves and Posakony, 2005, Lawrence, 2006, Atkey et al., 2006, Parker, 2006, Laplante and Nilson, 2006, Pallavi et al., 2006, Liu et al., 2006, Kim et al., 2006, Zinzen et al., 2006, Biemar et al., 2006, Brodu and Casanova, 2006, Cela and Llimargas, 2006, Mukherjee et al., 2006, Charroux et al., 2006, Sotillos and De Celis, 2005, Lee et al., 2002, Zeitlinger et al., 2007, Sandmann et al., 2007, Halfon and Arnosti, 2007, Stathopoulos and Levine, 2005, Galindo et al., 2005, Miura et al., 2006, Brown et al., 2006, Buszczak et al., 2007, Gomez et al., 2005, Beaver et al., 2008, Gebelein et al., 2008, Maeda et al., 2007, Molnar et al., 2006, Kim et al., 2004, Foltenyi et al., 2007, Hashimoto and Yamaguchi, 2006, Cowden and Levine, 2003, Georlette et al., 2007, Markstein et al., 2004, Bonds et al., 2007, Astigarraga et al., 2007, Qi et al., 2008, Zinzen et al., 2006, Escudero et al., 2007, Li-Kroeger et al., 2008, Yakoby et al., 2008, Ruel et al., 2007, Nishimura et al., 2007, Yakoby et al., 2008, Haecker et al., 2008, Li et al., 2008, Gilchrist et al., 2008, Hewitt et al., 1999, Iyadurai et al., 2008, Lilja et al., 2007, Maybeck and Röper, 2009, Zartman et al., 2008, Mao and Freeman, 2009, Jiang and Edgar, 2009, Huh et al., 2009, Krejcí et al., 2009, Birkholz et al., 2009, Angulo et al., 2004, DeLotto, 2001, Yan et al., 2009, Boettiger and Levine, 2009, Zartman et al., 2009, Lachance et al., 2009, Muse et al., 2007, Zeitlinger et al., 2007, Muse et al., 2007, Zeitlinger et al., 2007, Duong et al., 2008, Oishi et al., 2006, Li et al., 2007, Kagesawa et al., 2008, Birkholz et al., 2009, Klein et al., 2010, Wang et al., 2010, Jones et al., 2010, Witt et al., 2010, Figeac et al., 2010, Guichard et al., 2006, Yu et al., 2010, Tipping et al., 2010, Rendina et al., 2010, Lee et al., 1999, Chang et al., 2001, Marco et al., 2009, Beltran et al., 2007, Pilgram et al., 2011, Fakhouri et al., 2010, Ajuria et al., 2011, Crocker et al., 2010, Hogan et al., 2011, Cherbas et al., 2011, Gutzwiller et al., 2010, Jiang et al., 2011, Dworkin et al., 2011, Wojcinski et al., 2011, Uhl et al., 2010, Yasugi et al., 2010, Rousso et al., 2010, Ozdemir et al., 2011) Rho (McGovern et al., 2003, Zecca and Struhl, 2002, Ghiglione et al., 1999, Schweitzer et al., 1995, Yakoby et al., 2007, Brodu and Casanova, 2006, Yogev et al., 2007, Li-Kroeger et al., 2008, Kelkar and Dobberstein, 2009, Zhang et al., 2011) RHO (Colson, 1998.5.26, Colson, 1998.5.26, Brodu and Casanova, 2006) Rho-1 (Brossier, 2005, Adrain et al., 2004, Urban et al., 2002, Yogev et al., 2008) Rho1 (Gallio et al., 2004, Rogers et al., 2003, Williams et al., 2005, Chen et al., 2006, Williams and Hultmark, 2007, Webb et al., 2007, Yogev et al., 2007, Kunwar et al., 2008, Warner et al., 2010) rho1 (Urban et al., 2004, Gallio et al., 2002, Frankfort and Mardon, 2002, Frankfort et al., 2004, Tsruya et al., 2007, Pizette et al., 2009, Corl et al., 2009) rho-1 (Birkholz et al., 2004, Yang and Baker, 2003, Gallio et al., 2002, Ghiglione et al., 2002, Lee et al., 2001, Wasserman et al., 2000, Yogev et al., 2008) RHOb (Colson and Goldstein, 1999) rhom (Alexandre et al., 1999) rhomboid/veinlet (Li and White, 2003) ve (Campuzano, 2001, Crozatier et al., 2004, Johannes and Preiss, 2002, Sotillos and Campuzano, 2000, Hatini et al., 2000, Vasil'eva and Ratner, 2000, Garoia et al., 2000, Baonza et al., 2000, Palsson and Gibson, 2000, O'Grady and DeSalle, 2000, True et al., 1999, Greaves et al., 1999, Baonza and Garcia-Bellido, 1999, Roch et al., 1998, Cifuentes and Garcia-Bellido, 1997, de Celis et al., 1997, de Celis, 1997, Xiao et al., 1996, Gomez-Skarmeta and Modolell, 1996, Montagne et al., 1996, de Celis et al., 1995, Garcia-Bellido et al., 1994, Wessendorf et al., 1992, Robertson and Riviera, 1972, Nambu et al., 1991, Diaz-Benjumea and Garcia-Bellido, 1990, Diaz-Benjumea and Garcia-Bellido, 1990, Nambu et al., 1990, Diaz-Benjumea et al., 1989, Roberts and Evans-Roberts, 1979, ) Ve (Spurway, 1942)
Name Synonym	rhomboid (Jordan et al., 2005, Nibu et al., 2007, Nishimura et al., 2007, Atkey et al., 2006, Parker, 2006, Kim et al., 2006, Biemar et al., 2006, Dworkin and Gibson, 2006, Sotillos and De Celis, 2005, Urban, 2006, Baker, 2007, Takaesu et al., 2005, Galindo et al., 2005, Pribyl et al., 2003, Ward et al., 2006, Gomez et al., 2005, Nibu et al., 2003, Maeda et al., 2007, Kim et al., 2004, Foltenyi et al., 2007, Voas and Rebay, 2004, Cowden and Levine, 2003, Markstein et al., 2004, Lin et al., 2007, Bonds et al., 2007, Grueber et al., 2007, Astigarraga et al., 2007, Qi et al., 2008, Tadros et al., 2007, Escudero et al., 2007, Li-Kroeger et al., 2008, Nishimura et al., 2007, Haecker et al., 2008, O'Keefe et al., 2007, Chen et al., 2007, Li et al., 2008, Lilja et al., 2007, Crocker et al., 2008, Jiang and Edgar, 2009, Hurlbut et al., 2009, Krejcí et al., 2009, Birkholz et al., 2009, DeLotto, 2001, Terriente-Félix and de Celis, 2009, Corl et al., 2009, Yan et al., 2009, Oishi et al., 2006, Li et al., 2007, Birkholz et al., 2009, Guichard et al., 2006, Chung et al., 2009, Jones et al., 2010, Witt et al., 2010, Figeac et al., 2010, Lee et al., 1999, Chang et al., 2001, Buchon et al., 2010, Fakhouri et al., 2010, Ajuria et al., 2011, Jiang et al., 2011) Rhomboid (Kudoh, 1999, Li-Kroeger and Gebelein, 2006, Brodu and Casanova, 2006, Hashimoto and Yamaguchi, 2007, Anonymous, 2007, Li-Kroeger et al., 2008, Yamashita et al., 2005, Iyadurai et al., 2008, Buchon et al., 2009, Zartman et al., 2009) rhomboid-1 (McNeill et al., 2008, Nakamura and Matsuno, 2002, Wasserman et al., 2000, Hashimoto and Yamaguchi, 2007, Urban, 2006, Hashimoto and Yamaguchi, 2006, Mao and Freeman, 2009, Brodu et al., 2004) Rhomboid-1 (Freeman, 2003, Urban, 2001.10.16, Lee et al., 2001, Urban et al., 2001, Yogev et al., 2008, Tsruya et al., 2007) rhomboid1 (Matsuda et al., 2003, Maybeck and Röper, 2009, Pizette et al., 2009) Rhomboid 1 (Urban and Freeman, 2003) rhomboid/veinlet (Gomez-Skarmeta and Modolell, 1996) veinlet (Brumby et al., 2004, Sanson, 2001, Zimmerman et al., 2000, Crozatier et al., 2004, Palsson and Gibson, 2000, ) Veinlet (Norton, 2000)
Secondary FlyBase IDs
	FBgn0003251 FBgn0003972 FBgn0026834
References ( 746 )
Generate a list of	All references relating to this gene ( 746 )
List References by type	Research paper  ( 455 ) Supplementary material  ( 6 ) Review  ( 137 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 120 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Conference report  ( 3 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Book  ( 1 ) Automatic genome annotation  ( 1 ) Letter  ( 1 )
Recent research papers ( 28 )
	Ajuria et al., 2011, Development 138(5): 915--924 Capicua DNA-binding sites are general response elements for RTK signaling in Drosophila. [FBrf0212975] Cherbas et al., 2011, Genome Res. 21(2): 301--314 The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077] Dworkin et al., 2011, Genetics 187(4): 1171--1184 The effects of weak genetic perturbations on the transcriptome of the wing imaginal disc and its association with wing shape in Drosophila melanogaster. [FBrf0214376] Hogan et al., 2011, PLoS Genet. 7(2): e1001305 Two frizzled planar cell polarity signals in the Drosophila wing are differentially organized by the fat/dachsous pathway. [FBrf0213159] Jiang et al., 2011, Cell Stem Cell 8(1): 84--95 EGFR/Ras/MAPK Signaling Mediates Adult Midgut Epithelial Homeostasis and Regeneration in Drosophila. [FBrf0212694] McHale et al., 2011, Dev. Biol. 360(1): 230--240 Gene length may contribute to graded transcriptional responses in the Drosophila embryo. [FBrf0216627] Ozdemir et al., 2011, Genome Res. 21(4): 566--577 High resolution mapping of Twist to DNA in Drosophila embryos: Efficient functional analysis and evolutionary conservation. [FBrf0213359] Pilgram et al., 2011, J. Neurosci. 31(2): 492--500 The RhoGAP crossveinless-c Interacts with Dystrophin and Is Required for Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0212767] Sukhanova et al., 2011, Dev. Biol. 359(2): 190--198 RBF and Rno promote photoreceptor differentiation onset through modulating EGFR signaling in the Drosophila developing eye. [FBrf0216493] Wojcinski et al., 2011, Dev. Biol. 358(1): 168--180 DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop. [FBrf0215253] Zhang et al., 2011, Development 138(3): 455--464 Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis. [FBrf0212692] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Buchon et al., 2010, BMC Biol. 8: 152 Drosophila EGFR pathway coordinates stem cell proliferation and gut remodeling following infection. [FBrf0212840] Crocker et al., 2010, Nat. Commun. 1(7): 99 Dynamic evolution of precise regulatory encodings creates the clustered site signature of enhancers. [FBrf0212170] Fakhouri et al., 2010, Mol. Syst. Biol. 6: 341 Deciphering a transcriptional regulatory code: modeling short-range repression in the Drosophila embryo. [FBrf0209745] Figeac et al., 2010, Development 137(12): 1965--1973 Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway. [FBrf0210887] Gutzwiller et al., 2010, Dev. Biol. 348(2): 231--243 Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen. [FBrf0212265] Jones et al., 2010, J. Cell Sci. 123(13): 2179--2189 Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation. [FBrf0211056] Klein et al., 2010, Dev. Biol. 337(2): 458--470 The proprioceptive and contractile systems in Drosophila are both patterned by the EGR family transcription factor Stripe. [FBrf0209777] Rendina et al., 2010, Genetics 186(1): 167--181 Bap170, a Subunit of the Drosophila PBAP Chromatin Remodeling Complex, Negatively Regulates the EGFR Signaling. [FBrf0211845] Rousso et al., 2010, Development 137(20): 3427--3437 Generation of distinct signaling modes via diversification of the Egfr ligand-processing cassette. [FBrf0211899] Tipping et al., 2010, EMBO J. 29(19): 3222--3235 β-arrestin Kurtz inhibits MAPK and Toll signalling in Drosophila development. [FBrf0211995] Uhl et al., 2010, Dev. Biol. 343(1-2): 154--166 Comparing anterior and posterior Hox complex formation reveals guidelines for predicting cis-regulatory elements. [FBrf0214949] Wang et al., 2010, PLoS ONE 5(7): e11498 NELF Potentiates Gene Transcription in the Drosophila Embryo. [FBrf0211281] Warner et al., 2010, Curr. Biol. 20(8): 677--686 The Cdc42/Par6/aPKC Polarity Complex Regulates Apoptosis-Induced Compensatory Proliferation in Epithelia. [FBrf0211164] Witt et al., 2010, Dev. Biol. 344(2): 1060--1070 Atonal, Senseless, and Abdominal-A regulate rhomboid enhancer activity in abdominal sensory organ precursors. [FBrf0211413] Yasugi et al., 2010, Development 137(19): 3193--3203 Coordinated sequential action of EGFR and Notch signaling pathways regulates proneural wave progression in the Drosophila optic lobe. [FBrf0211765] Yu et al., 2010, PLoS Genet. 6(5): e1000969 Affecting Rhomboid-3 function causes a dilated heart in adult Drosophila. [FBrf0210954] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...