E(sev)2B, Grb2, l(2)k13809, crkl, Su(sev)R1
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.49
Interacts with autophosphorylated sev via SH2 domain and Sos, Dos and Dab via SH3 domains. Binds to tyrosine phosphorylated Dab via the SH2 domain. Required for functional and morphological integrities of the scolopidia, sensory neurons and the antennal mechanosensory and motor center (AMMC) brain neuropil (PubMed:30610177).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\drk using the Feature Mapper tool.
drk protein is strongly expressed in the mushroom body alpha, beta and gamma lobes and spur. It is weakly expressed in the mushroom body alpha and beta lobes and calyx.
drk protein was detected in lysates from larvae, pupae, and adults. Immunolocalization studies indicate that it is expressed at high levels in all cells of the eye-antennal disc in third instar larvae, primarily in the apical membranes. Behind the morphogenetic furrow, it is observed at higher levels in a subpopulation of developing photoreceptor cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\drk in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: drk l(2)k13809
drk affects 90 minute memory formation or stability within the mushroom bodies.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. S2R+ cells are unaffected.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
A high affinity binding site for the drk SH3 domain maps to the Sos product tail. The N-terminal drk SH3 domain is primarily responsible for binding to the tail of the Sos product in vitro, and for signalling to the Ras85D product in vivo.
Wild type drk function is essential for signaling by the sev receptor tyrosine kinase. Its biological activity correlates with binding of its SH2 domain to activated receptor tyrosine kinases and concomitant localization to the plasma membrane. In vitro, drk binds the C terminal tail of the Sos (a Ras guanine nucleotide releasing protein) gene product through the drk SH3 domain, thereby linking receptor tyrosine kinases to Ras activation.
drk is required for photoreceptor development and is a suppressor of the Egfr phenotype to restore the eye to a nearly normal appearance.