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Gene Dmel\drk

General Information
Symbol	Dmel\drk	Species	D. melanogaster
Name	downstream of receptor kinase	Annotation symbol	CG6033
Feature type	protein_coding_gene	FlyBase ID	FBgn0004638
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	E(sev)2B, GRB2, crkl, l(2)k13809, l(2)10626
Genomic Location
Chromosome (arm)	2R	Recombination map	2-70
Cytogenetic map	50A13-50A13	Sequence location	2R:9,382,727..9,390,342 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene downstream of receptor kinase is referred to in FlyBase by the symbol Dmel\drk (CG6033, FBgn0004638). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; SH3/SH2 adaptor activity; sevenless binding. There is experimental evidence for 11 unique biological process terms, many of which group under: biological regulation; anatomical structure development; associative learning; learning or memory; learning; regulation of developmental process; cellular component organization or biogenesis; memory; embryonic development via the syncytial blastoderm; organ morphogenesis. 37 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; larval abdominal segment; abdominal lateral pentascolopidial chordotonal organ lch5; larval abdominal segment 5; adult segment; abdominal segment 7; abdominal 2 ventral monoscolopidial chordotonal organ vch1; imaginal precursor; external compound sense organ; abdominal 7 ventral monoscolopidial chordotonal organ vch1. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: Neutrophil cytosol factor 2 p67phox; SH2 motif; Src homology-3 domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625475_s_at completely aligns to an exonic region common to each of the 6 FlyBase-annotated transcript isoforms of drk. Gene sequence location is 2R:9382727..9390342.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Sequence features BKN29233 HFA07606 Controlled Vocabulary Terms mushroom body spur calyx of adult mushroom body intense
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 50A13-50A13   Limits computationally determined from genome sequence between P{EP}DpEP358 and P{lacW}drkk13809&P{EP}mip120EP606
Experimentally Determined Cytological Location
Cytogenetic map Notes References 50A12-50A14     (Simon, 1993.6.25) 50A12-50A14   (determined by in situ hybridisation)   (Spradling et al., 1999) 50A-50B   (determined by in situ hybridisation)   (Roch et al., 1998) 50A12-50A14   (determined by in situ hybridisation)   (Perrimon et al., 1996) 50A12-50A14     (BDGP Project Members, 1994-1999) 50A-50A   (determined by in situ hybridisation)   (Olivier et al., 1993) 50A12-50A14   (determined by in situ hybridisation)   (Simon et al., 1993)
Experimentally Determined Recombination Data
Location	2-70 (Simon et al., 1991, Simon et al., 1993)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\drk for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) drk-RA FBtr0087696  NM_057510   2740   211 drk-RB FBtr0087695  NM_165994   2671   211 drk-RC FBtr0087694  NM_165995   2642   211 drk-RD FBtr0087698  NM_165996   3254   211 drk-RE FBtr0087693  NM_165997   2543   211 drk-RF FBtr0087697  NM_165998   2573   211
Additional Transcript Data & Comments
Reported size (kB)	2.65 (longest cDNA) (Simon et al., 1993) 1.156 (longest cDNA) (Olivier et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein drk-PA   FBpp0086815   24.4   211   5.01     NP_476858   AAF58368 drk-PB   FBpp0086814   24.4   211   5.01     NP_725302   AAM68581 drk-PC   FBpp0086813   24.4   211   5.01     NP_725303   AAM68582 drk-PD   FBpp0086817   24.4   211   5.01     NP_725304   AAM68583 drk-PE   FBpp0086812   24.4   211   5.01     NP_725305   AAM68584 drk-PF   FBpp0086816   24.4   211   5.01     NP_725306   AAM68585
Additional Polypeptide Data & Comments
Reported size (kDa)	211 (aa); 24 (kD) (Olivier et al., 1993) 211 (aa) (Simon et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Neutrophil cytosol factor 2 p67phox (IPR000108) SH2 motif (IPR000980) Src homology-3 domain (IPR001452)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392845   AA439814   AA440267   AA694685   AA697657   AE013599 AAF58368   AE013599 AAM68581   AE013599 AAM68582   AE013599 AAM68583   AE013599 AAM68584   AE013599 AAM68585   AC006488   AC007452   AI238839   AI456978   AI511885   AI512809   AI518041   AI520499   AQ026070   AQ026071   AQ073620   AW942213   AY061142 AAL28690   AY135053 AAN17564   AY135054 AAN17565   AY135055 AAN17566   AY135056 AAN17567   AY135057 AAN17568   AY135058 AAN17569   AY135059 AAN17570   AY135060 AAN17571   AY135061 AAN17572   AY135062 AAN17573   AY135063 AAN17574   AY135064 AAN17575   AY135065 AAN17576   AY135066 AAN17577   AY135067 AAN17578   AY135068 AAN17579   AY135069 AAN17580   AY135070 AAN17581   AY135071 AAN17582   AY135072 AAN17583   AY135073 AAN17584   AY135074 AAN17585   BE978775   BF485771   BF490340   BF492036   BF492253   BF498885   BG632851   BG634143   BI163089   BI167733   BI170022   BI170085   BI171438   BI173876   BI212779   BI213199   BI214822   BI229921   BI234310   BI234759   BI235224   BI235470   BI235660   BI235932   BI235935   BI236031   BI236163   BI236555   BI240939   BI241017   BI357446   BI358386   BI358593   BI363135   BI368987   BI370125   BI371693   BI372594   BI373633   BI374937   BI375439   BI482276   BI483638   BI484196   BI578425   BI578841   BI580035   BI580180   BI580584   BP547055   BP547503   BP547849   CA807378   CA807732   CK131380   CO179600   CO186133   CO187877   CO191510   CO194386   CO263789   CO268995   CO272033   CO278299   CO279287   CO281348   CO281596   CO282118   CO282155   CO282671   CO284565   CO285253   CO293188   CO293538   CO300550   CO300612   CO301053   CO301446   CO308787   CO310219   CO314565   CO316709   CO319247   CO324172   CO324202   CO333259   CO333282   CO333978   EC056107   EC061972   EC062058   EC062767   EC066860   EC068427   EC071144   EC072426   EC074675   EC085431   EC087385   EC089162   EC196018   EC199554   EC205067   EC208731   EC211492   EC211611   EC212588   EC216792   EC221490   EC222486   EC225859   EC228811   EC229535   EC234462   EC234605   EC235665   EC238864   EC239833   EC243150   EC244171   EC244602   EC245303   EC245727   EC245769   EC246077   EC248192   G00788   L12446 AAA28898   L13173
UniProtKB/Swiss-Prot	Q08012
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   imaginal disc   (Olivier et al., 1993) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Olivier et al., 1993) third instar larval stage   eye-antennal disc   (Olivier et al., 1993)
Additional Descriptive Data
	drk transcripts are expressed strongly and uniformly in the eye-imaginal disc of 3rd instar larvae. They are also observed in other imaginal discs and in embryos. (Olivier et al., 1993)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   photoreceptor cell | presumptive   (Olivier et al., 1993) adult stage     (Olivier et al., 1993) immunolocalization Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   eye-antennal disc   • apical plasma membrane (Olivier et al., 1993) pupal stage     (Olivier et al., 1993) adult stage   mushroom body calyx | faint & restricted   (Moressis et al., 2009) adult pedunculus   (Moressis et al., 2009) ring neuron R4   (Moressis et al., 2009) mushroom body alpha-lobe   (Moressis et al., 2009) mushroom body alpha'-lobe | faint   (Moressis et al., 2009) mushroom body beta-lobe   (Moressis et al., 2009) mushroom body beta'-lobe | faint   (Moressis et al., 2009) mushroom body gamma-lobe   (Moressis et al., 2009) adult antennal lobe glomerulus   (Moressis et al., 2009) mushroom body alpha-lobe | intense   (Crittenden et al., 1998) mushroom body beta-lobe | intense   (Crittenden et al., 1998) mushroom body gamma-lobe | intense   (Crittenden et al., 1998) mushroom body spur | intense   (Crittenden et al., 1998) mushroom body alpha-lobe | faint   (Crittenden et al., 1998) mushroom body beta-lobe | faint   (Crittenden et al., 1998) calyx of adult mushroom body | faint   (Crittenden et al., 1998) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	drk protein is observed in apical membranes. (Olivier et al., 1993) drk protein is strongly expressed in the mushroom body alpha, beta and gamma lobes and spur. It is weakly expressed in the mushroom body alpha and beta lobes and calyx. (Crittenden et al., 1998)
Marker for
Subcellular Localization
CV Term	plasma membrane | apical (Olivier et al., 1993)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0004638    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0004638 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0004638 expression level Developmental Stage   Expression Level embryo 00-02hr    2205 embryo 02-04hr    4401 embryo 04-06hr    6567 embryo 06-08hr    5027 embryo 08-10hr    5892 embryo 10-12hr    3909 embryo 12-14hr    4101 embryo 14-16hr    1285 embryo 16-18hr    1800 embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr    1858 larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6    2107 larva L3 puffstage 7-9    2848 white prepupae new    2522 white prepupae 12hr    4890 white prepupae 24hr    4867 pupae 2d postWPP    2799 pupae 3d postWPP    1928 pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day    2045 adult female 30day    1626 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2205) embryo 02-04hr  (4401) embryo 04-06hr  (6567) embryo 06-08hr  (5027) embryo 08-10hr  (5892) embryo 10-12hr  (3909) embryo 12-14hr  (4101) embryo 14-16hr  (1285) embryo 16-18hr  (1800) embryo 18-20hr  (1145) embryo 20-22hr  (1182) embryo 22-24hr  (1858) larva L1  (783) larva L2  (1284) larva L3 12hr old  (884) larva L3 puffstage 1-2  (1303) larva L3 puffstage 3-6  (2107) larva L3 puffstage 7-9  (2848) white prepupae new  (2522) white prepupae 12hr  (4890) white prepupae 24hr  (4867) pupae 2d postWPP  (2799) pupae 3d postWPP  (1928) pupae 4d postWPP  (1319) adult male 01day  (969) adult male 05day  (989) adult male 30day  (768) adult female 01day  (860) adult female 05day  (2045) adult female 30day  (1626) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2205) embryo 02-04hr  (4401) embryo 04-06hr  (6567) embryo 06-08hr  (5027) embryo 08-10hr  (5892) embryo 10-12hr  (3909) embryo 12-14hr  (4101) embryo 14-16hr    1285 embryo 16-18hr  (1800) embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr  (1858) larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6  (2107) larva L3 puffstage 7-9  (2848) white prepupae new  (2522) white prepupae 12hr  (4890) white prepupae 24hr  (4867) pupae 2d postWPP  (2799) pupae 3d postWPP  (1928) pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day  (2045) adult female 30day  (1626) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2205 embryo 02-04hr    4401 embryo 04-06hr    6567 embryo 06-08hr    5027 embryo 08-10hr    5892 embryo 10-12hr    3909 embryo 12-14hr    4101 embryo 14-16hr    1285 embryo 16-18hr    1800 embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr    1858 larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6    2107 larva L3 puffstage 7-9    2848 white prepupae new    2522 white prepupae 12hr    4890 white prepupae 24hr    4867 pupae 2d postWPP    2799 pupae 3d postWPP    1928 pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day    2045 adult female 30day    1626 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2205 embryo 02-04hr    4401 embryo 04-06hr    6567 embryo 06-08hr    5027 embryo 08-10hr    5892 embryo 10-12hr    3909 embryo 12-14hr    4101 embryo 14-16hr    1285 embryo 16-18hr    1800 embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr    1858 larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6    2107 larva L3 puffstage 7-9    2848 white prepupae new    2522 white prepupae 12hr    4890 white prepupae 24hr    4867 pupae 2d postWPP    2799 pupae 3d postWPP    1928 pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day    2045 adult female 30day    1626 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0004638 expression level Developmental Stage   Expression Level embryo 00-02hr    2205 embryo 02-04hr    4401 embryo 04-06hr    6567 embryo 06-08hr    5027 embryo 08-10hr    5892 embryo 10-12hr    3909 embryo 12-14hr    4101 embryo 14-16hr    1285 embryo 16-18hr    1800 embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr    1858 larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6    2107 larva L3 puffstage 7-9    2848 white prepupae new    2522 white prepupae 12hr    4890 white prepupae 24hr    4867 pupae 2d postWPP    2799 pupae 3d postWPP    1928 pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day    2045 adult female 30day    1626 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2205) embryo 02-04hr  (4401) embryo 04-06hr  (6567) embryo 06-08hr  (5027) embryo 08-10hr  (5892) embryo 10-12hr  (3909) embryo 12-14hr  (4101) embryo 14-16hr  (1285) embryo 16-18hr  (1800) embryo 18-20hr  (1145) embryo 20-22hr  (1182) embryo 22-24hr  (1858) larva L1  (783) larva L2  (1284) larva L3 12hr old  (884) larva L3 puffstage 1-2  (1303) larva L3 puffstage 3-6  (2107) larva L3 puffstage 7-9  (2848) white prepupae new  (2522) white prepupae 12hr  (4890) white prepupae 24hr  (4867) pupae 2d postWPP  (2799) pupae 3d postWPP  (1928) pupae 4d postWPP  (1319) adult male 01day  (969) adult male 05day  (989) adult male 30day  (768) adult female 01day  (860) adult female 05day  (2045) adult female 30day  (1626) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  2205 embryo 02-04hr  (4401) embryo 04-06hr  (6567) embryo 06-08hr  (5027) embryo 08-10hr  (5892) embryo 10-12hr  (3909) embryo 12-14hr  (4101) embryo 14-16hr    1285 embryo 16-18hr  1800 embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr  1858 larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6  2107 larva L3 puffstage 7-9  (2848) white prepupae new  (2522) white prepupae 12hr  (4890) white prepupae 24hr  (4867) pupae 2d postWPP  (2799) pupae 3d postWPP  1928 pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day  2045 adult female 30day  1626 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2205 embryo 02-04hr    4401 embryo 04-06hr    6567 embryo 06-08hr    5027 embryo 08-10hr    5892 embryo 10-12hr    3909 embryo 12-14hr    4101 embryo 14-16hr    1285 embryo 16-18hr    1800 embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr    1858 larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6    2107 larva L3 puffstage 7-9    2848 white prepupae new    2522 white prepupae 12hr    4890 white prepupae 24hr    4867 pupae 2d postWPP    2799 pupae 3d postWPP    1928 pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day    2045 adult female 30day    1626 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2205 embryo 02-04hr    4401 embryo 04-06hr    6567 embryo 06-08hr    5027 embryo 08-10hr    5892 embryo 10-12hr    3909 embryo 12-14hr    4101 embryo 14-16hr    1285 embryo 16-18hr    1800 embryo 18-20hr    1145 embryo 20-22hr    1182 embryo 22-24hr    1858 larva L1    783 larva L2    1284 larva L3 12hr old    884 larva L3 puffstage 1-2    1303 larva L3 puffstage 3-6    2107 larva L3 puffstage 7-9    2848 white prepupae new    2522 white prepupae 12hr    4890 white prepupae 24hr    4867 pupae 2d postWPP    2799 pupae 3d postWPP    1928 pupae 4d postWPP    1319 adult male 01day    969 adult male 05day    989 adult male 30day    768 adult female 01day    860 adult female 05day    2045 adult female 30day    1626 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0004638    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0004638 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0004638 expression level Tissue   Expression Level Larval Central Nervous System    753.1 Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body    625.1 Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca    581.8 Adult InseminatedFemale Spermatheca    599.8 Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (753.1) Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body  (625.1) Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca  (581.8) Adult InseminatedFemale Spermatheca  (599.8) Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    753.1 Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body    625.1 Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca    581.8 Adult InseminatedFemale Spermatheca    599.8 Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    753.1 Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body    625.1 Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca    581.8 Adult InseminatedFemale Spermatheca    599.8 Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0004638 expression level Tissue   Expression Level Larval Central Nervous System    753.1 Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body    625.1 Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca    581.8 Adult InseminatedFemale Spermatheca    599.8 Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  753.1 Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body  625.1 Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca  581.8 Adult InseminatedFemale Spermatheca  599.8 Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    753.1 Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body    625.1 Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca    581.8 Adult InseminatedFemale Spermatheca    599.8 Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    753.1 Larval Midgut    142.4 Larval Hindgut    260.2 Larval Malpighian Tubules    215.6 Larval Fat Body    625.1 Larval Salivary Gland    349.5 Larval Trachea    259.5 Larval Carcass    229.475 Adult Head    260.2 Adult Eye    160.8 Adult Brain    394.3 Adult Thoracic-Abdominal Ganglion    362.7 Adult Crop    474.7 Adult Midgut    126.4 Adult Hindgut    273 Adult Malpighian Tubules    178.4 Adult Fat Body    190.3 Adult Salivary Gland    330.5 Adult Heart    247.5 Adult VirginFemale Spermatheca    581.8 Adult InseminatedFemale Spermatheca    599.8 Adult Ovary    105.4 Adult Testis    69.4 Adult Male Accessory Gland    460.4 Adult Carcass    149.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    260.2   NA  Eye    160.8   NA  Brain    394.3   753.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    362.7   NA  Crop    474.7   142.4  Midgut    126.4   260.2  Hindgut    273   215.6  Malpighian Tubules    178.4   625.1  Fat Body    190.3   349.5  Salivary Gland    330.5   NA  Heart    247.5   259.5  Trachea    NA   NA  VirginFemale Spermatheca    581.8   NA  InseminatedFemale Spermatheca    599.8   NA  Ovary    105.4   NA  Testis    69.4   NA  Male Accessory Gland    460.4   229.475  Carcass    149.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004638 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6033-RE FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for drk Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal drkCB02974 lethal | embryonic stage | fully | rescuable maternal effect drk10626 lethal | larval stage | recessive drk10626 lethal | pupal stage (with drkE(sev)2B) drkR1 lethal | pupal stage (with drkR1) drkE(sev)2B lethal | pupal stage | recessive drkk13809 lethal | recessive drk10626 drke0A drkE(sev)2B drkk02401 drkk03106 drkk13913 drkk15709 Other Phenotypes Allele learning defective drk10626 drke0A drkk02401 drkR1 drkΔP24 learning defective, with Scer\GAL4c739 drkdsRNA.Sym.Scer\UAS learning defective, with Scer\GAL4c747 drkdsRNA.Sym.Scer\UAS learning defective, with Scer\GAL4Mef2.247 drkdsRNA.Sym.Scer\UAS learning defective, with Scer\GAL4Tab2-201Y drkdsRNA.Sym.Scer\UAS memory defective drkΔP24 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 drkEP2477 visible | dominant drke0A drkk02401 drkKG03077 visible | recessive drkk13809 Phenotype manifest in Allele abdomen | posterior drk10626 abdominal 1 lateral pentascolopidial chordotonal organ lch5 drkunspecified abdominal 1 ventral monoscolopidial chordotonal organ vch1 drkunspecified abdominal 2 lateral pentascolopidial chordotonal organ lch5 drkunspecified abdominal 2 ventral monoscolopidial chordotonal organ vch1 drkunspecified abdominal 3 lateral pentascolopidial chordotonal organ lch5 drkunspecified abdominal 3 ventral monoscolopidial chordotonal organ vch1 drkunspecified abdominal 4 lateral pentascolopidial chordotonal organ lch5 drkunspecified abdominal 4 ventral monoscolopidial chordotonal organ vch1 drkunspecified abdominal 5 lateral pentascolopidial chordotonal organ lch5 drkunspecified abdominal 5 ventral monoscolopidial chordotonal organ vch1 drkunspecified abdominal 6 lateral pentascolopidial chordotonal organ lch5 drkunspecified abdominal 6 ventral monoscolopidial chordotonal organ vch1 drkunspecified abdominal 7 lateral pentascolopidial chordotonal organ lch5 drkunspecified abdominal 7 ventral monoscolopidial chordotonal organ vch1 drkunspecified anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 drkEP2477 dorsal air sac primordium | somatic clone drkΔP24 embryonic/first instar larval cuticle drk10626 head drk10626 imaginal disc drkk03106 drkk13809 intersegmental nerve, with Scer\GAL4elav-C155 drkEP2477 macrochaeta drkR1 photoreceptor cell R7 drke0A wing drkKG03077 wing | posterior drkk02401 drkKG03077 wing vein drkR1 drkk13809 wing vein | ectopic drkR1 drkKG03077
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of drk Class Mutagen Stocks Known lesion drke0A antimorphic allele - genetic evidence 2 Yes drkk02401 P-element activity 2 -- drk10626 P-element activity 1 -- drk14-7 ethyl methanesulfonate 1 -- drk6 hypomorphic allele - genetic evidence ethyl methanesulfonate 1 -- drkk03106 P-element activity 1 -- drkk13809 P-element activity 1 -- drkk13913 P-element activity 1 -- drkKG03077 1 -- drkCB02974 P-element activity 0 Yes drkCPTI002249 piggyBac transposase 0 Yes drkE(sev)2B ethyl methanesulfonate 0 Yes drkEDA 0 -- drkEP2477 P-element activity 0 Yes drkk15709 P-element activity 0 -- drkR1 loss of function allele, hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes drkS11 0 -- drkTZ160 0 -- drkunspecified 0 -- drkΔP24 0 Yes
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of drk Class Mutagen Stocks Known lesion drkJF01377 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes drkJF02717 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes drkKK109233 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes drkAct5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes drkdsRNA.cIa in vitro construct - RNAi 0 Yes drkdsRNA.Sym.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes drkE(sev)2B.sev in vitro construct - regulatory fusion 0 Yes drkG199R.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes drkGD8656 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes drkhs.PM 0 Yes drkhs.sev in vitro construct - regulatory fusion 0 Yes drkP49L.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes drkP49L.G199R.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes drkR85K.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes drkSH3(N)SH2.hs.sev in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes drkW189A.hs.sev in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes drkW36A.hs.sev in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)8-104 (Vaizel-Ohayon and Schejter, 1999) Df(2R)cnn (Heuer et al., 1995) Df(2R)CX1 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)vg-B (Spradling et al., 1999, BDGP Project Members, 1994-1999, Heuer et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8656} NA P{KK109233} NA P{Sym-UAS-drk} NA P{TRiP.JF01377} NA P{TRiP.JF02717} NA heat-shock construct P{hs-drk.M} NA P{sE-drkW36A} NA P{sE-drkW189A} NA P{sE-drkwt} NA P{SH3(N)SH2} NA characterization construct P{sev-drk} NA P{UAS-Cbl.L.RING::drk.SH2} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}drkk02401 No P{lacW}drkk03106 No P{lacW}drkk13809 No P{lacW}drkk13913 No P{lacW}drkk15709 No P{lacW}l(2)SH0563SH0563 No P{PZ}drk10626 No insertion of mobile activating element P{EP}drkEP2477 NA miscellaneous insertions P{PTT-GB}drkCB02974 NA P{SUPor-P}drkKG03077 NA PBac{754.P.FSVS-0}drkCPTI002249 NA
Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Cbl (Wang and Pai, 2011) inferred from physical interaction with Dab AND inferred from physical interaction with Sos (Le and Simon, 1998) inferred from physical interaction with Sos (Olivier et al., 1993) inferred from physical interaction with UniProtKB:Q9VZZ9 (assigned by UniProtKB) (Feller et al., 2002) sevenless binding inferred from physical interaction with sev (Olivier et al., 1993) SH3/SH2 adaptor activity inferred from physical interaction with Sos AND inferred from physical interaction with sev (Olivier et al., 1993)
Biological Process
CV term Evidence References
actin filament organization inferred from mutant phenotype (Kiger et al., 2003) associative learning inferred from mutant phenotype (Moressis et al., 2009) embryonic development via the syncytial blastoderm inferred from mutant phenotype (Perrimon et al., 1996) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Dworkin and Gibson, 2006) negative regulation of epidermal growth factor-activated receptor activity inferred from genetic interaction with Cbl (Wang and Pai, 2011) olfactory learning inferred from mutant phenotype (Moressis et al., 2009) positive regulation of R7 cell differentiation inferred from genetic interaction with sev (Almudi et al., 2010) Ras protein signal transduction inferred from physical interaction with Sos AND inferred from physical interaction with human EGFR AND inferred from sequence or structural similarity with human GRB2 (Olivier et al., 1993) regulation of cell shape inferred from mutant phenotype (Kiger et al., 2003) sevenless signaling pathway inferred from genetic interaction with sev AND inferred from physical interaction with Sos AND inferred from physical interaction with sev (Olivier et al., 1993) short-term memory inferred from mutant phenotype (Moressis et al., 2009)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Olivier et al., 1993)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
SH3/SH2 adaptor activity inferred from sequence or structural similarity with mouse Grb2 (FlyBase, 1992-) non-traceable author statement (Kumar and Moses, 2000, Swiss-Prot Project Members, 1995.2.1)
Biological Process
CV term Evidence References
anterior/posterior axis specification, embryo traceable author statement (Baek and Lee, 1999) memory non-traceable author statement (Sacharidou and Skoulakis, 2002) olfactory learning non-traceable author statement (Sacharidou and Skoulakis, 2002) Ras protein signal transduction inferred from sequence or structural similarity with mouse Grb2 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1995.2.1) sevenless signaling pathway traceable author statement (Kumar and Moses, 2000) torso signaling pathway traceable author statement (Baek and Lee, 1999)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions drk allele Gene References drk10626 CycE (Brumby et al., 2004) drke0A B-H1 (Hayashi et al., 1998) Src42A (Takahashi et al., 1996) Src64B (Cooper et al., 1996) svp (Kramer et al., 1995) drkE(sev)2B sev (Simon et al., 1993) svp (Begemann et al., 1995) tor (Doyle and Bishop, 1993) drkk02401 vap (Botella et al., 2003) drkR1 sev (Olivier et al., 1993) drkTZ160 csw (Firth et al., 2000) tor (Li and Li, 2003) drkΔP24 Src42A (Zhang et al., 1999) unspecified Egfr (Read et al., 2005) Ret (Read et al., 2005) sev (Simon et al., 1991)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62256 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004638 DroID - A comprehensive database of gene and protein interactions. • FBgn0004638 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36497
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11180 Dere\GG22486 Dgri\GH22624 Dmoj\GI21333 Dper\GL17069 Dpse\GA19310 Dsec\GM20273 Dsim\drk Dvir\GJ19679 Dwil\GK10660 Dyak\GE13357
Curated drosophilid orthologs	Dpse\GA19310   Dsim\drk
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL013786-PA Aedes aegypti (mosquito) AAEL014394-PA Aedes aegypti (mosquito) AGAP011768-PA Anopheles gambiae (mosquito) XP_623354.1 Apis mellifera (honey bee) XP_001951296.1 Acyrthosiphon pisum (pea aphid) 220041 Branchiostoma floridae (lancelet) XP_001893200.1 Brugia malayi (roundworm nematode) ENSBTAP00000006216 Bos taurus (bovine) CN11341 Caenorhabditis brenneri (small nematode) CBP18823 Caenorhabditis briggsae (nematode) CE01784 Caenorhabditis elegans (worm) ENSCAFP00000026928 Canis familiaris (dog) ENSCINP00000011733 Ciona intestinalis (sea squirt) JA00528 Caenorhabditis japonica (nematode) CPIJ000806 Culex pipens (mosquito) ENSCPOP00000004161 Cavia porcellus (guinea pig) RP22848 Caenorhabditis remanei (nematode) ENSCSAVP00000006921 Ciona savignyi (sedentary tunicate) 93061 Capitella spl (polychaete worm) ENSDARP00000055472 Danio rerio (zebra fish) ENSECAP00000007886 Equus caballus (horse) ENSGACP00000009150 Gymnopilus aculeatus (mushroom) ENSGALP00000012993 Gallus gallus (chicken) ENSP00000339007 Homo sapiens (human) ISCW016244-PA Ixodes scapularis (deer tick) 196407 Lottia gigantea (owl limpet) ENSMODP00000009105 Monodelphis domestica (gray opossum) ENSMMUP00000007041 Macaca mulatta (rhesus monkey) ENSMUSP00000102106 Mus musculus (house mouse) 229989 Nematostella vectensis (starlet anemone) XP_001605040.1 Nasonia vitripennis (wasp) ENSOANP00000023109 Ornithorynchus anatinus (platypus) ENSORLP00000019098 Oryzias latipes (japanese killifish) PHUM000559-PA Pediculus humanus (human body louse) PP12750 Pristionchus pacificus (nematode) ENSPPYP00000009686 Pongo pygmaeus (orangutan) ENSPTRP00000016382 Pan troglodytes (chimpanzee) ENSRNOP00000005347 Rattus norvegicus (norway rat) Smp_062950 Schistosoma mansoni (trematode parasite) XP_786023.2 Strongylocentrotus purpuratus (purple sea urchin) 25635 Trichoplax adhaerens (metazoa) XP_969998.1 Tribolium castaneum (red flour beetle) ENSTNIP00000014198 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000020658 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000027387 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13944 LH16236 ISCW016244 FBgn0231036 FBgn0212673 FBgn0206821 FBgn0063984 FBgn0175156 FBgn0079307 FBgn0154673 FBgn0144063 FBgn0130082 FBgn0114657 FBgn0088220 CPIJ000806 BGIBMGA007310 ACYPI009973 GB15989 AGAP011768 AAEL014394 AAEL013786 PB20142 PHUM423150 GLEAN_06549
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	5691 sev14; drke0A/CyO 27563 y1 v1; P{TRiP.JF02717}attP2 12378 P{PZ}drk10626 cn1/CyO; ry506 10372 y1 w67c23; P{lacW}drkk02401/CyO 13943 y1 w67c23; P{SUPor-P}drkKG03077 31494 y1 v1; P{TRiP.JF01377}attP2/TM3, Ser1 27623 w1118; drk6/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7
Kyoto	108368 sev14; drke0A/CyO 102254 y1 w67c23 ; P{lacW}drkk03106 / CyO 102897 y1 w67c23 ; P{lacW}drkk13809 / CyO 102904 y1 w67c23 ; P{lacW}drkk13913 / CyO
VDRC	v105498 P{KK109233}VIE-260B
	More stocks available...
Genomic Clones ( 1 )
	BACR36J03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 137 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD12029
Other clones	DPiM_LD12029
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT13295 AT19817 AT26682 AT28906 AT29160 AT30234 GH14963 GM01280 HL02943 LD14483 LD14912 LD37168 LD37445 LD40679 LD43441 LD44868 LD46519 RE02405 RE08184 RE11357 RE11436 RE13217 RE17004 RE18027 RE18553 RE20951 RE27814 RE29995 RE30515 RE31041 RE31320 RE31565 RE31891 RE31894 RE32017 RE32180 RE32626 RE37905 RE37983 RE43939 RE45131 RE45417 RE47874 RE55057 RE56536 RE58636 RE59593 RE60975 RE62632 RE63255 RE64945 RE66556 RE67257 RE72294 RE72771 RE74156 RE74325 RE74796
Other clones	17318 20208 28513 30783 42510 42672 44276 44383 44971 52763 53305 54515 54884 57021 57087 57706 58445 58584 64637 73026 73906 83493 102388 117917 156561 172929 173035 194687 281071 282955 bs82g04 CK01.16B.D5 CK01.16B.F2 CK01.34D.C1 CK01.76C.E12 CK01.122A.F2 CK01.126B.D7 CK01.128C.H6 EC31249 EC38449 EC056107 EK016112 EK034532 EK059939 EK093253 EK105702 EK129662 EK133951 EK136439 EK156916 EK157650 EK159559 EK159647 EK160938 EK166317 EK168615 EK178531 EK178807 EK179956 EK180960 EK192909 EK192955 EK202027 EK203262 EK220809 EK237030 EK259340 EK265704 EK277431 EK306324 EK306406 EK308522 ESG017c.B21.1_G01 ESG019b.E21.2_C11 FGH112H11 FGH117F07 FGH121D02
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004638 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36497
Antibody Information
	polyclonal (Olivier et al., 1993, Moressis et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: drk l(2)k13809 (Beaton, 1999.12.12)
Additional comments	Not allelic to shot. (Gregory and Brown, 1998) The vulval induction defects of C.elegans Cele\sem-5 mutants are efficiently rescued by drk, but the Soc phenotype of C.elegans Cele\sem-5 mutants is only poorly rescued by drk. (Stern et al., 1993)
Other Comments
	drk affects 90 minute memory formation or stability within the mushroom bodies. (Moressis et al., 2009) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. S2R+ cells are unaffected. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) Abnormal eye development caused by ectopic expression of an activated form of Src64B is alleviated by dominant-negative mutations of csw or drk. (Cooper et al., 1996) dos functions upstream or independent of Ras85D and defines a signalling pathway that is independent of direct binding of the drk SH2/SH3 adaptor protein to the sev receptor tyrosine kinase. (Raabe et al., 1996) Membrane localization of Sos protein is independent of drk function. (Karlovich et al., 1995) A high affinity binding site for the drk SH3 domain maps to the Sos product tail. The N-terminal drk SH3 domain is primarily responsible for binding to the tail of the Sos product in vitro, and for signalling to the Ras85D product in vivo. (Raabe et al., 1995) Tyr2546 in the cytoplasmic tail of the sev protein is required for drk protein binding though a mutation in this site does not completely block sev function in vivo. (Raabe et al., 1995) Wild type drk function is essential for signaling by the sev receptor tyrosine kinase. Its biological activity correlates with binding of its SH2 domain to activated receptor tyrosine kinases and concomitant localization to the plasma membrane. In vitro, drk binds the C terminal tail of the Sos (a Ras guanine nucleotide releasing protein) gene product through the drk SH3 domain, thereby linking receptor tyrosine kinases to Ras activation. (Olivier et al., 1993) drk is required for photoreceptor development and is a suppressor of the Egfr phenotype to restore the eye to a nearly normal appearance. (Simon et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057510 NM_165994 NM_165995 NM_165996 NM_165997 NM_165998 RefSeq (Proteins) • NP_476858 NP_725302 NP_725303 NP_725304 NP_725305 NP_725306 DDBJ / EMBL / GenBank • AA392845 AAA28898 AAF58368 AAL28690 AAM68581 AAM68582 AAM68583 AAM68584 AAM68585 AAN17564 AAN17565 AAN17566 AAN17567 AAN17568 AAN17569 AAN17570 AAN17571 AAN17572 AAN17573 AAN17574 AAN17575 AAN17576 AAN17577 AAN17578 AAN17579 AAN17580 AAN17581 AAN17582 AAN17583 AAN17584 AAN17585 AC006488 AC007452 AI238839 AQ026070 AQ026071 AQ073620 AW942213 AY061142 AY135053 AY135054 AY135055 AY135056 AY135057 AY135058 AY135059 AY135060 AY135061 AY135062 AY135063 AY135064 AY135065 AY135066 AY135067 AY135068 AY135069 AY135070 AY135071 AY135072 AY135073 AY135074 BG632851 G00788 L12446 L13173 Entrez Gene - A searchable database of RefSeq genes. • 36497 UniProtKB/Swiss-Prot • Q08012
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Neutrophil cytosol factor 2 p67phox (IPR000108) SH2 motif (IPR000980) Src homology-3 domain (IPR001452)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62256 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004638 DroID - A comprehensive database of gene and protein interactions. • FBgn0004638 DRSC - Results from RNAi screens. • FBgn0004638 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004638 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6033-RE FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004638 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36497 InParanoid A subset of ortholog calls from InParanoid. • AAEL013786-PA Aedes aegypti (mosquito) AAEL014394-PA Aedes aegypti (mosquito) AGAP011768-PA Anopheles gambiae (mosquito) XP_623354.1 Apis mellifera (honey bee) XP_001951296.1 Acyrthosiphon pisum (pea aphid) 220041 Branchiostoma floridae (lancelet) XP_001893200.1 Brugia malayi (roundworm nematode) ENSBTAP00000006216 Bos taurus (bovine) CN11341 Caenorhabditis brenneri (small nematode) CBP18823 Caenorhabditis briggsae (nematode) CE01784 Caenorhabditis elegans (worm) ENSCAFP00000026928 Canis familiaris (dog) ENSCINP00000011733 Ciona intestinalis (sea squirt) JA00528 Caenorhabditis japonica (nematode) CPIJ000806 Culex pipens (mosquito) ENSCPOP00000004161 Cavia porcellus (guinea pig) RP22848 Caenorhabditis remanei (nematode) ENSCSAVP00000006921 Ciona savignyi (sedentary tunicate) 93061 Capitella spl (polychaete worm) ENSDARP00000055472 Danio rerio (zebra fish) ENSECAP00000007886 Equus caballus (horse) ENSGACP00000009150 Gymnopilus aculeatus (mushroom) ENSGALP00000012993 Gallus gallus (chicken) ENSP00000339007 Homo sapiens (human) ISCW016244-PA Ixodes scapularis (deer tick) 196407 Lottia gigantea (owl limpet) ENSMODP00000009105 Monodelphis domestica (gray opossum) ENSMMUP00000007041 Macaca mulatta (rhesus monkey) ENSMUSP00000102106 Mus musculus (house mouse) 229989 Nematostella vectensis (starlet anemone) XP_001605040.1 Nasonia vitripennis (wasp) ENSOANP00000023109 Ornithorynchus anatinus (platypus) ENSORLP00000019098 Oryzias latipes (japanese killifish) PHUM000559-PA Pediculus humanus (human body louse) PP12750 Pristionchus pacificus (nematode) ENSPPYP00000009686 Pongo pygmaeus (orangutan) ENSPTRP00000016382 Pan troglodytes (chimpanzee) ENSRNOP00000005347 Rattus norvegicus (norway rat) Smp_062950 Schistosoma mansoni (trematode parasite) XP_786023.2 Strongylocentrotus purpuratus (purple sea urchin) 25635 Trichoplax adhaerens (metazoa) XP_969998.1 Tribolium castaneum (red flour beetle) ENSTNIP00000014198 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000020658 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000027387 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36497 modMine - Data generated by the modENCODE project. • FBgn0004638 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13944 LH16236 ISCW016244 FBgn0231036 FBgn0212673 FBgn0206821 FBgn0063984 FBgn0175156 FBgn0079307 FBgn0154673 FBgn0144063 FBgn0130082 FBgn0114657 FBgn0088220 CPIJ000806 BGIBMGA007310 ACYPI009973 GB15989 AGAP011768 AAEL014394 AAEL013786 PB20142 PHUM423150 GLEAN_06549
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	24/1 (BDGP Project Members, 1994-1999) CG6033 (Kraut et al., 2001, Kraut et al., 2001, Rogers et al., 2003, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Cammarato et al., 2011) crkl (Kumar and Moses, 2000, Simon et al., 1992) downstream of receptor kinase (Edwards et al., 2006) drk (Dworkin and Gibson., 2006, Jordan et al., 2005, Dworkin and Gibson, 2006, Acevedo et al., 2007, Buszczak et al., 2007, Bergeret et al., 2001, MacDougall et al., 2004, Tollinger et al., 2003, Yakoby et al., 2008, Cabernard and Affolter, 2005, Yan et al., 2009, Kaufman, 2009.6.18, Zeitlinger et al., 2007, Oishi et al., 2006, Oishi et al., 2009, Li et al., 2009, Gim et al., 2001, Sims et al., 2009, Fernández-Ayala et al., 2010, Moressis et al., 2009, Almudi et al., 2010, Marsh et al., 2007, Settle et al., 2003, Kundrotas and Karshikoff, 2004, Crittenden et al., 1998, Dworkin et al., 2011) Drk (Sundaram, 2005, Rogers et al., 2003, Kockel et al., 2001, Cork and Purugganan, 2004, Avery et al., 2004, Claeys et al., 2002, Oldham and Hafen, 2003, Borland et al., 2001, Duchek et al., 2001, Luzi et al., 2000, Baek and Lee, 1999, Placzek and Skaer, 1999, den Hertog, 1999, Foukas et al., 1998, Hime et al., 1997, Okabe and Okano, 1997, MacDougall and Waterfield, 1996, Bonfini et al., 1996, Dominguez and Hafen, 1996, Karlovich et al., 1995, Raabe et al., 1993, Diaz-Benjumea and Hafen, 1994, Boedigheimer et al., 1993, Chang et al., 2007, Rogers et al., 2003, Voas and Rebay, 2004, Yatsenko et al., 2007, Dawes-Hoang and Wieschaus, 2001, Robertson et al., 2000, Almudi et al., 2009, Bezsonova et al., 2005, Serio et al., 2010, Wang and Pai, 2011) DRK (Sacharidou and Skoulakis, 2002, Roche, 2000, Le and Simon, 1998, Slack et al., 2010, Moressis et al., 2009, Ferris et al., 2006) Drk/Grb2 (Ghabrial et al., 2003) E(sev)2B (Kumar and Moses, 2000, Takahashi et al., 1996, Kramer and Cagan, 1994, Yamamoto, 1993, Simon et al., 1992, Simon et al., 1993, Simon et al., 1991, ) GRB2 (Yamaguchi et al., 2006) Grb2 (Dawes-Hoang and Wieschaus, 2001, Yan et al., 2009, Csiszar et al., 2010) Grb-2 (Settle et al., 2003) Grb2/drk (Wilson and Leptin, 2000) Grb/drk (Friedman and Perrimon, 2006) l(2)10626 (Gregory and Brown, 1998, Perrimon et al., 1996) l(2)k13809 (Liebl, 1999, Roch et al., 1998, ) P1112 (Liebl, 1999) Su(sev)R1 (Kumar and Moses, 2000, )
Name Synonym	downstream of receptor kinase (Edwards et al., 2006, Jordan et al., 2005, Moressis et al., 2009, Oishi et al., 2006, Cammarato et al., 2011) Downstream of receptor kinase (Gim et al., 2001) downstream of receptor kinases (Dworkin and Gibson, 2006) downstream receptor kinase (Li et al., 2009) Enhancer of sevenless-2B
Secondary FlyBase IDs
	FBgn0021869
References ( 215 )
Generate a list of	All references relating to this gene ( 215 )
List References by type	Research paper  ( 110 ) Supplementary material  ( 12 ) Review  ( 58 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 17 ) FlyBase analysis  ( 2 ) Computer file  ( 3 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 8 )
	Dworkin et al., 2011, Genetics 187(4): 1171--1184 The effects of weak genetic perturbations on the transcriptome of the wing imaginal disc and its association with wing shape in Drosophila melanogaster. [FBrf0214376] Horn et al., 2011, Nat. Methods 8(4): 341--346 Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352] Wang and Pai, 2011, PLoS ONE 6(2): e17097 D-Cbl Binding to Drk Leads to Dose-Dependent Down-Regulation of EGFR Signaling and Increases Receptor-Ligand Endocytosis. [FBrf0213073] Almudi et al., 2010, J. Cell Sci. 123(22): 3857--3862 Competition between SOCS36E and Drk modulates Sevenless receptor tyrosine kinase activity. [FBrf0212242] Csiszar et al., 2010, Mol. Cell. Biol. 30(8): 2017--2027 A novel conserved phosphotyrosine motif in the Drosophila fibroblast growth factor signaling adaptor Dof with a redundant role in signal transmission. [FBrf0210374] Fernández-Ayala et al., 2010, PLoS ONE 5(1): e8549 Gene expression in a Drosophila model of mitochondrial disease. [FBrf0209705] Serio et al., 2010, Cell Host Microbe 7(5): 388--398 Defining a Core Set of Actin Cytoskeletal Proteins Critical for Actin-Based Motility of Rickettsia. [FBrf0210774] Slack et al., 2010, PLoS Genet. 6(3): e1000881 Regulation of lifespan, metabolism, and stress responses by the Drosophila SH2B protein, Lnk. [FBrf0210418] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...