• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\mit(1)15

General Information
Symbol	Dmel\mit(1)15	Species	D. melanogaster
Name	mitotic 15	Annotation symbol	CG9900
Feature type	protein_coding_gene	FlyBase ID	FBgn0004643
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	zw10, l(1)zw10, ZW-10, EG:BACR7C10.3
Genomic Location
Chromosome (arm)	X	Recombination map	1-1.2
Cytogenetic map	3A6-3A7	Sequence location	X:2,498,224..2,500,860 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene mitotic 15 is referred to in FlyBase by the symbol Dmel\mit(1)15 (CG9900, FBgn0004643). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: cell cycle; mitotic sister chromatid segregation; neurogenesis. 33 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; sensillum; adult mesothoracic segment; spermatocyte; cell cycle; integumentary plate; nuclear part; eo support cell; anterior vertical bristle; cellular component organization or biogenesis; spermatozoon; cellular process involved in reproduction; external compound sense organ. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: RZZ complex, subunit Zw10. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1634604_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of mit(1)15. Gene sequence location is X:2498224..2500860.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	mit(1)15 Semilethal as males or homozygous females; surviving flies have reduced roughened eyes, bristles on the head and thorax sometimes missing, abnormal wings with thin texture and thickened veins; both sexes sterile. Up to 100 fold increase in the incidence of mwh clones per wing in mit15;mwh/+ flies raised at sub-restrictive temperature. Half of all metaphases in larval ganglion cells are hyperploid for one or more chromosomes; hyperploidy for nearly all combinations of sex chromosomes and autosomes are observed. Chromosome breakage seen in less than 2% of cells. Cytological observations in mit(1)1515, mit(1)153 and mit(1)154 at either restrictive or permissive temperature.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	Sequence features HFA18793 BKN20532
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 3A6-3A7   Limits computationally determined from genome sequence between P{EP}eghEP804 and P{EP}sggEP1576
Experimentally Determined Cytological Location
Cytogenetic map Notes References 3A8-3A8     (Williams, 1992.2.6)
Experimentally Determined Recombination Data
Location	1-1.2 (Smith et al., 1985) 1-1.2 +/- 0.4 (Fahmy and Fahmy, 1959)
Left of (cM)	l(1)3Ah (Judd et al., 1972)
Right of (cM)	wds (Judd et al., 1972)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mit(1)15 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone SD07771 appears problematic: unspliced intron Atypical cDNA: retained intron (SD07771).
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mit(1)15-RB FBtr0070441  NM_080162   2576   721 mit(1)15-RC FBtr0100272  NM_001038739   2637   485
Additional Transcript Data & Comments
Reported size (kB)	2.6 (northern blot) (Williams et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mit(1)15-PB   FBpp0070425   82.3   721   4.92     NP_524901   AAF45794 mit(1)15-PC   FBpp0099660   55.0   485   4.61     NP_001033828   ABC67171
Additional Polypeptide Data & Comments
Reported size (kDa)	721 (aa); 85 (kD observed) (Williams et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RZZ complex, subunit Zw10 (IPR009361)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246420   AA695973   AA942029   AA950879   AE014298 AAF45794   AE014298 ABC67171   AI134283   AI296678   AI518187   AI519227   AI534763   AI542261   AI543123   AL121804 CAB65854   AL138972 CAB72295   AW944527   AY069831 AAL39976   BE976639   BF491711   BF494537   BG632548   BG634759   BI168051   BI240819   BI243613   BI365599   BI372995   BI577011   BI577666   BI604444   BI615008   BI615931   BI624334   BI637450   BI637722   BI641572   CK658540   CO270840   CO323195   DN153809   EC063181   EC082766   EC197350   EV587347   X64390 CAB76122
UniProtKB/Swiss-Prot	Q9W4X9
UniProtKB/TrEMBL	Q8T9C2 Q2PE30
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- pupal stage     (Williams et al., 1992) adult stage | female     (Williams et al., 1992)
Additional Descriptive Data
	Levels of mit(1)15 transcript are high in embryos and in adult females, decline in first and second instar larvae, and increase again in third instar larvae and in early pupae. (Williams et al., 1992)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference spermatogenesis & adult stage   spermatocyte   (Williams et al., 1996) onion stage spermatid   (Williams et al., 1996) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 16     (Williams et al., 1992) Comment:reference states 0-16 hr AEL embryonic stage 4     (Williams et al., 1992) third instar larval stage     (Williams et al., 1992)
Additional Descriptive Data
	The mit(1)15 protein shows a dynamic localization pattern during the embryonic cell cycle. During interphase, the protein is excluded from the nucleus. It migrates to the nuclear zone during prometaphase, and is found in a filamentous (possibly kinetochore microtubule-associated) structure during metaphase. At anaphase, mit(1)15 protein is found at or near kinetochores. (Williams et al., 1992) The intracellular localization of mit(1)15 protein in the spermatocyte changes several times during the two meiotic divisions. Localization is uniform and diffuse in the nucleus during meiotic prophase and early prometaphase. During both meiotic divisions of spermatogenesis, the protein is found in the kinetochore regions of chromosomes at late prometaphase, moves along kinetochore microtubules during metaphase, and returns to the kinetochore at the start of anaphase. Some protein is found in the spindle midbody during telophase. (Williams et al., 1996)
Marker for
Subcellular Localization
CV Term	nucleus, cytoplasm (Williams et al., 1992) nucleus kinetochore midbody microtubule (Williams et al., 1996)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0004643    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0004643 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0004643 expression level Developmental Stage   Expression Level embryo 00-02hr    7815 embryo 02-04hr    1306 embryo 04-06hr    3498 embryo 06-08hr    3233 embryo 08-10hr    1979 embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new    1456 white prepupae 12hr    1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day    2629 adult female 05day    4457 adult female 30day    4829 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (7815) embryo 02-04hr  (1306) embryo 04-06hr  (3498) embryo 06-08hr  (3233) embryo 08-10hr  (1979) embryo 10-12hr  (1333) embryo 12-14hr  (803) embryo 14-16hr  (875) embryo 16-18hr  (671) embryo 18-20hr  (532) embryo 20-22hr  (337) embryo 22-24hr  (340) larva L1  (721) larva L2  (1041) larva L3 12hr old  (800) larva L3 puffstage 1-2  (952) larva L3 puffstage 3-6  (1348) larva L3 puffstage 7-9  (1377) white prepupae new  (1456) white prepupae 12hr  (1487) white prepupae 24hr  (1375) pupae 2d postWPP  (812) pupae 3d postWPP  (481) pupae 4d postWPP  (622) adult male 01day  (716) adult male 05day  (776) adult male 30day  (877) adult female 01day  (2629) adult female 05day  (4457) adult female 30day  (4829) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (7815) embryo 02-04hr    1306 embryo 04-06hr  (3498) embryo 06-08hr  (3233) embryo 08-10hr  (1979) embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new  1456 white prepupae 12hr  1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day  (2629) adult female 05day  (4457) adult female 30day  (4829) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    7815 embryo 02-04hr    1306 embryo 04-06hr    3498 embryo 06-08hr    3233 embryo 08-10hr    1979 embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new    1456 white prepupae 12hr    1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day    2629 adult female 05day    4457 adult female 30day    4829 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    7815 embryo 02-04hr    1306 embryo 04-06hr    3498 embryo 06-08hr    3233 embryo 08-10hr    1979 embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new    1456 white prepupae 12hr    1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day    2629 adult female 05day    4457 adult female 30day    4829 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0004643 expression level Developmental Stage   Expression Level embryo 00-02hr    7815 embryo 02-04hr    1306 embryo 04-06hr    3498 embryo 06-08hr    3233 embryo 08-10hr    1979 embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new    1456 white prepupae 12hr    1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day    2629 adult female 05day    4457 adult female 30day    4829 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (7815) embryo 02-04hr  (1306) embryo 04-06hr  (3498) embryo 06-08hr  (3233) embryo 08-10hr  (1979) embryo 10-12hr  (1333) embryo 12-14hr  (803) embryo 14-16hr  (875) embryo 16-18hr  (671) embryo 18-20hr  (532) embryo 20-22hr  (337) embryo 22-24hr  (340) larva L1  (721) larva L2  (1041) larva L3 12hr old  (800) larva L3 puffstage 1-2  (952) larva L3 puffstage 3-6  (1348) larva L3 puffstage 7-9  (1377) white prepupae new  (1456) white prepupae 12hr  (1487) white prepupae 24hr  (1375) pupae 2d postWPP  (812) pupae 3d postWPP  (481) pupae 4d postWPP  (622) adult male 01day  (716) adult male 05day  (776) adult male 30day  (877) adult female 01day  (2629) adult female 05day  (4457) adult female 30day  (4829) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (7815) embryo 02-04hr    1306 embryo 04-06hr  (3498) embryo 06-08hr  (3233) embryo 08-10hr  1979 embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new  1456 white prepupae 12hr  1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day  (2629) adult female 05day  (4457) adult female 30day  (4829) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    7815 embryo 02-04hr    1306 embryo 04-06hr    3498 embryo 06-08hr    3233 embryo 08-10hr    1979 embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new    1456 white prepupae 12hr    1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day    2629 adult female 05day    4457 adult female 30day    4829 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    7815 embryo 02-04hr    1306 embryo 04-06hr    3498 embryo 06-08hr    3233 embryo 08-10hr    1979 embryo 10-12hr    1333 embryo 12-14hr    803 embryo 14-16hr    875 embryo 16-18hr    671 embryo 18-20hr    532 embryo 20-22hr    337 embryo 22-24hr    340 larva L1    721 larva L2    1041 larva L3 12hr old    800 larva L3 puffstage 1-2    952 larva L3 puffstage 3-6    1348 larva L3 puffstage 7-9    1377 white prepupae new    1456 white prepupae 12hr    1487 white prepupae 24hr    1375 pupae 2d postWPP    812 pupae 3d postWPP    481 pupae 4d postWPP    622 adult male 01day    716 adult male 05day    776 adult male 30day    877 adult female 01day    2629 adult female 05day    4457 adult female 30day    4829 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0004643    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0004643 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0004643 expression level Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0004643 expression level Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    154.6 Larval Midgut    50.9 Larval Hindgut    51.8 Larval Malpighian Tubules    99.6 Larval Fat Body    56.7 Larval Salivary Gland    307.8 Larval Trachea    109.95 Larval Carcass    60.025 Adult Head    47.4 Adult Eye    55.875 Adult Brain    74.8 Adult Thoracic-Abdominal Ganglion    69.4 Adult Crop    46.8 Adult Midgut    47.2 Adult Hindgut    42.6 Adult Malpighian Tubules    61.2 Adult Fat Body    49.8 Adult Salivary Gland    135 Adult Heart    54.775 Adult VirginFemale Spermatheca    61.3 Adult InseminatedFemale Spermatheca    76.2 Adult Ovary    369.6 Adult Testis    18.1 Adult Male Accessory Gland    191.6 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    47.4   NA  Eye    55.875   NA  Brain    74.8   154.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    69.4   NA  Crop    46.8   50.9  Midgut    47.2   51.8  Hindgut    42.6   99.6  Malpighian Tubules    61.2   56.7  Fat Body    49.8   307.8  Salivary Gland    135   NA  Heart    54.775   109.95  Trachea    NA   NA  VirginFemale Spermatheca    61.3   NA  InseminatedFemale Spermatheca    76.2   NA  Ovary    369.6   NA  Testis    18.1   NA  Male Accessory Gland    191.6   60.025  Carcass    49.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004643 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9900-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for mit(1)15 Stages(s) 1-3 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal mit(1)154 mit(1)155 mit(1)1516 mit(1)1517 lethal, with Scer\GAL41407 mit(1)15GD13807 lethal | embryonic stage | maternal effect mit(1)154 lethal | embryonic stage | recessive mit(1)154 mit(1)1516 mit(1)1517 lethal | heat sensitive mit(1)1515 lethal | larval stage | recessive mit(1)153 lethal | pupal stage | recessive mit(1)154 mit(1)1516 mit(1)1517 lethal | recessive mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)1511 mit(1)1512 mit(1)1516 mit(1)1518 mit(1)15A223 mit(1)15M27 mit(1)15M59 mit(1)15M68 mit(1)15T12 semi-lethal | polyphasic mit(1)154 mit(1)155 viable mit(1)15abe viable, with Scer\GAL4pnr-MD237 mit(1)15GD13807 Sterility Allele female sterile mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)155 mit(1)156 mit(1)157 mit(1)158 mit(1)159 mit(1)1510 mit(1)15abe male fertile mit(1)15abe male sterile mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)155 mit(1)156 mit(1)157 mit(1)158 mit(1)159 mit(1)1510 mit(1)1516 mit(1)1517 Other Phenotypes Allele cytokinesis defective | recessive mit(1)154 mit(1)1516 mit(1)1517 meiotic cell cycle defective mit(1)154 mit(1)1516 mit(1)1517 mitotic cell cycle defective mit(1)154 mit(1)1516 mit(1)1517 neuroanatomy defective | larval stage, with Scer\GAL41407 mit(1)15GD13807 visible mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)155 mit(1)15abe visible, with Scer\GAL4pnr-MD237 mit(1)15GD13807 wild-type mit(1)15+t4.6 Phenotype manifest in Allele anterior orbital bristle mit(1)154 mit(1)155 anterior vertical bristle mit(1)154 mit(1)155 dorsocentral bristle mit(1)154 mit(1)155 egg | germline clone | maternal effect mit(1)154 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 mit(1)15GD13807 eye mit(1)151 mit(1)1511 mit(1)1512 mit(1)152 mit(1)153 mit(1)154 mit(1)155 mit(1)15abe female reproductive system | maternal effect mit(1)154 ganglion mother cell | larval stage, with Scer\GAL41407 mit(1)15GD13807 macrochaeta & head mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)155 macrochaeta & thorax mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)155 meiotic cell cycle mit(1)1516 middle orbital bristle mit(1)154 mit(1)155 mitotic anaphase mit(1)1516 nuclear chromosome mit(1)1516 nuclear chromosome | embryonic stage | germline clone mit(1)1516 mit(1)1517 mit(1)154 nucleus | embryonic stage | germline clone mit(1)1516 mit(1)1517 mit(1)154 ocellar bristle mit(1)154 mit(1)155 onion stage spermatid mit(1)1516 mit(1)1517 mit(1)154 posterior orbital bristle mit(1)154 mit(1)155 posterior vertical bristle mit(1)154 mit(1)155 scutellar bristle mit(1)154 mit(1)155 secondary spermatocyte mit(1)1516 spermatid mit(1)1516 mit(1)1517 mit(1)154 spermatocyte mit(1)1516 spermatozoon mit(1)154 mit(1)155 testis mit(1)1516 mit(1)1517 mit(1)154 trichogen cell, with Scer\GAL4pnr-MD237 mit(1)15GD13807 wing mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)155 mit(1)15abe wing vein mit(1)151 mit(1)152 mit(1)153 mit(1)154 mit(1)155 wing vein L4 mit(1)15abe
Classical Alleles ( 28 )
For All Classical Alleles Show
Allele of mit(1)15 Class Mutagen Stocks Known lesion mit(1)155 amorphic allele - genetic evidence X ray ethylenimine 2 -- mit(1)1515 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- mit(1)1516 amorphic allele - genetic evidence, loss of function allele spontaneous 0 Yes mit(1)1517 loss of function allele, amorphic allele - genetic evidence spontaneous 0 -- mit(1)154 amorphic allele - genetic evidence, loss of function allele ethylenimine 0 -- mit(1)1510 triethylenemelamine 0 -- mit(1)1511 methyl methanesulfonate 0 -- mit(1)1512 methyl methanesulfonate 0 -- mit(1)1513 methyl methanesulfonate 0 -- mit(1)1514 ethyl methanesulfonate 0 -- mit(1)1518 hycanthon methanesulfonate 0 -- mit(1)151 N-methyl-N'-nitro-N-nitrosoguanidine 0 -- mit(1)152 ethyl methanesulfonate 0 -- mit(1)153 X ray ethylenimine 0 -- mit(1)156 ethyl methanesulfonate 0 -- mit(1)157 ethyl methanesulfonate 0 -- mit(1)158 ethyl methanesulfonate 0 -- mit(1)159 triethylenemelamine 0 -- mit(1)15A223 0 -- mit(1)15abe 2-chloroethyl methanesulfonate 0 -- mit(1)15M27 methyl methanesulfonate 0 -- mit(1)15M59 methyl methanesulfonate 0 -- mit(1)15M68 methyl methanesulfonate 0 -- mit(1)15NKu1 urethane 0 -- mit(1)15NKx1 X ray 0 -- mit(1)15NKx3 X ray 0 -- mit(1)15T12 methyl methanesulfonate 0 -- mit(1)15unspecified 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mit(1)15 Class Mutagen Stocks Known lesion mit(1)15GD13807 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mit(1)15+t4.6 in vitro construct - genomic fragment 0 Yes mit(1)15KK100436 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mit(1)15SCA9 in vitro construct - genomic fragment 0 Yes mit(1)15T:Ivir\HA1 in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)7 (Nomura and Kurokawa, 1997) Df(1)64c4 (Judd et al., 1972, Thierry-Mieg, 1982) Df(1)HM3 (Kramers et al., 1983) Df(1)HM406 (Kramers et al., 1983) Df(1)K95 (Judd et al., 1972, Thierry-Mieg, 1982) Df(1)L126 (Nomura and Kurokawa, 1997) Df(1)NKx2 (Nomura and Kurokawa, 1997) Df(1)TEM1 (Lim and Snyder, 1974, Kramers et al., 1983, Kramers et al., 1991) Df(1)TEM7 (Lim and Snyder, 1974, Liu and Lim, 1975, Thierry-Mieg, 1982, Paradi et al., 1983, Kramers et al., 1983, Kramers et al., 1991) Df(1)TEM75 (Lim and Snyder, 1974) Df(1)TEM501 (Lim and Snyder, 1974, Kramers et al., 1983, Kramers et al., 1991) Df(1)w258-11 (Judd et al., 1972, Thierry-Mieg, 1982) Df(1)w258-42 (Judd et al., 1972, Thierry-Mieg, 1982) Df(1)w258-48 (Kramers et al., 1983, Kramers et al., 1991) Df(1)w-N71a (Judd et al., 1972) Df(1)w-rJ1 (Judd et al., 1972, Lim and Snyder, 1974, Liu and Lim, 1975, Thierry-Mieg, 1982, Paradi et al., 1983, Kramers et al., 1983, Smith et al., 1985, Kramers et al., 1991, Nomura and Kurokawa, 1997) Df(1)X12 (Judd et al., 1972, Thierry-Mieg, 1982) Df(1)z-deb54 (Goldberg et al., 1989) Df(1)z-deb62 (Goldberg et al., 1989) Df(1)z-deb65 (Goldberg et al., 1989) Df(1)z-deb72 (Goldberg et al., 1989) Df(1)z-deb81 (Goldberg et al., 1989) Df(1)z-deb85 (Goldberg et al., 1989) Df(1)z-deb311 (Goldberg et al., 1989) Df(1)z-deb511 (Goldberg et al., 1989) Df(1)z-EM41 (Montgomery et al., 1991) Df(1)z-EM42 (Montgomery et al., 1991) Df(1)z-EM43 (Montgomery et al., 1991) Df(1)z-EM44 (Montgomery et al., 1991) Df(1)z-EM45 (Montgomery et al., 1991) Df(1)z-EM46 (Montgomery et al., 1991)
Duplicated in	Dp(1;2;Y)w+ (Judd et al., 1972, Kramers et al., 1983, Kramers et al., 1991) Dp(1;2)w+70h (Judd et al., 1972) Dp(1;3)w+67k (Judd et al., 1972) Dp(1;Y)y267g19.1 (Lefevre, 1981)
Not disrupted in	Df(1)62d18 (Judd et al., 1972) Df(1)62g18 (Judd et al., 1972, Thierry-Mieg, 1982) Df(1)64f1 (Judd et al., 1972, Thierry-Mieg, 1982, Lin and Wolfner, 1989) Df(1)64j4 (Judd et al., 1972, Thierry-Mieg, 1982, Lin and Wolfner, 1989) Df(1)65j26 (Judd et al., 1972, Thierry-Mieg, 1982) Df(1)202 (Nomura and Kurokawa, 1997) Df(1)304 (Nomura and Kurokawa, 1997) Df(1)N-8 (Lin and Wolfner, 1989, Kramers et al., 1991) Df(1)N-54l9 (Kramers et al., 1991) Df(1)N-264-105 (Kramers et al., 1991) Df(1)Pgd-kz (Kramers et al., 1991) Df(1)Sp2F-3A (Schalet, 1986) Df(1)TEM6 (Lim and Snyder, 1974) Df(1)TEM202 (Liu and Lim, 1975, Paradi et al., 1983, Kramers et al., 1983, Kramers et al., 1991) Df(1)TEM304 (Lim and Snyder, 1974, Liu and Lim, 1975, Paradi et al., 1983, Kramers et al., 1983, Kramers et al., 1991) Df(1)w55j (Lin and Wolfner, 1989) Df(1)w67k30 (Kramers et al., 1991) Df(1)w258-45 (Judd et al., 1972, Thierry-Mieg, 1982, Lin and Wolfner, 1989) Df(1)w-rJ2 (Judd et al., 1972) Df(1)z-deb3 (Goldberg et al., 1989) Df(1)z-deb42 (Goldberg et al., 1989) Df(1)z-deb59 (Goldberg et al., 1989) Df(1)z-deb87 (Goldberg et al., 1989) Df(1)z-deb92 (Goldberg et al., 1989) Df(1)z-deb910 (Goldberg et al., 1989)
Not duplicated in	Dp(1;2)51b (Kramers et al., 1991) Dp(1;3)N264-58 (Judd et al., 1972) Dp(1;3)wm49a (Judd et al., 1972) Dp(1;3)wvco (Schalet, 1986) Dp(1;4)wm65g (Judd et al., 1972)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13807} NA P{KK100436} NA characterization construct P{mit(1)15.3HA} NA P{mit(1)15+tWa} NA P{SCA9} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Doc{}mit(1)1516 NA
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell cycle inferred from mutant phenotype (Kiger et al., 2003) mitotic sister chromatid segregation inferred from mutant phenotype (Scaerou et al., 2001) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
kinetochore inferred from direct assay (Scaerou et al., 2001) kinetochore microtubule inferred from direct assay (Scaerou et al., 2001)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromosome segregation non-traceable author statement (Swiss-Prot Project Members, 2000.5.1) meiotic anaphase I non-traceable author statement (Gonzalez and Bornens, 2000) mitotic cell cycle spindle assembly checkpoint non-traceable author statement (Gonzalez and Bornens, 2000) traceable author statement (Lee and Orr-Weaver, 2003) regulation of meiosis non-traceable author statement (Gonzalez and Bornens, 2000)
Cellular Component
CV term Evidence References
condensed chromosome outer kinetochore non-traceable author statement (Blower and Karpen, 2001) cytoplasm non-traceable author statement (Swiss-Prot Project Members, 2000.5.1) kinetochore non-traceable author statement (Gonzalez and Bornens, 2000, Swiss-Prot Project Members, 2000.5.1) kinetochore microtubule non-traceable author statement (Swiss-Prot Project Members, 2000.5.1) nucleus inferred from electronic annotation with InterPro:IPR009361 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 2000.5.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mit(1)15 allele Gene References mit(1)1517 asp (Basto et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 71022 DroID - A comprehensive database of gene and protein interactions. • FBgn0004643 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47874
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22219 Dere\GG12940 Dgri\mit(1)15 Dmoj\GI16458 Dper\GL13061 Dpse\mit(1)15 Dsec\GM19232 Dsim\GD24733 Dvir\GJ15859 Dwil\GK16273 Dyak\GE16265
Curated drosophilid orthologs	Dgri\mit(1)15 (Starr et al., 1997) Dmir\CG9900 (Thornton, 2005.12.20) Dpse\mit(1)15 (Starr et al., 1997)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL011103-PA Aedes aegypti (mosquito) AGAP004467-PA Anopheles gambiae (mosquito) XP_001121600.1 Apis mellifera (honey bee) At2g32900.1 Arabidopsis thaliana (wall cress) 213083 Branchiostoma floridae (lancelet) XP_001891601.1 Brugia malayi (roundworm nematode) BGIBMGA005798-PA Bombyx mori (silk worm) ENSBTAP00000026733 Bos taurus (bovine) CE04434 Caenorhabditis elegans (worm) ENSCAFP00000020211 Canis familiaris (dog) ENSCINP00000018123 Ciona intestinalis (sea squirt) CPIJ018520 Culex pipens (mosquito) ENSCPOP00000004585 Cavia porcellus (guinea pig) ENSCSAVP00000016808 Ciona savignyi (sedentary tunicate) 219679 Capitella spl (polychaete worm) DDB0190842 Dictyostelium discoideum (amoeba) ENSDARP00000020574 Danio rerio (zebra fish) ENSECAP00000009910 Equus caballus (horse) ENSGACP00000027445 Gymnopilus aculeatus (mushroom) ENSGALP00000011993 Gallus gallus (chicken) ENSP00000200135 Homo sapiens (human) ISCW010250-PA Ixodes scapularis (deer tick) 225294 Lottia gigantea (owl limpet) ENSMODP00000017739 Monodelphis domestica (gray opossum) ENSMMUP00000031313 Macaca mulatta (rhesus monkey) ENSMUSP00000034803 Mus musculus (house mouse) XP_001605971.1 Nasonia vitripennis (wasp) ENSOANP00000022557 Ornithorynchus anatinus (platypus) ENSORLP00000018055 Oryzias latipes (japanese killifish) PHUM010164-PA Pediculus humanus (human body louse) PP27469 Pristionchus pacificus (nematode) ENSPPYP00000004454 Pongo pygmaeus (orangutan) 552728 Populus trichocarpa (black cottonwood) ENSPTRP00000007380 Pan troglodytes (chimpanzee) ENSRNOP00000010595 Rattus norvegicus (norway rat) 4882387 Sorghum bicolor (sorghum) XP_001180688.1 Strongylocentrotus purpuratus (purple sea urchin) 51689 Trichoplax adhaerens (metazoa) XP_973305.1 Tribolium castaneum (red flour beetle) ENSTNIP00000010462 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000019635 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000049699 Xenopus tropicalis (frog) YALI0A07689g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB19316 NV14663 LH11835 ISCW010250 FBgn0233802 FBgn0218275 FBgn0203046 FBgn0196054 FBgn0174133 JGI_V11_64073 FBgn0022854 FBgn0157601 FBgn0150667 FBgn0139206 FBgn0125358 FBgn0022892 FBgn0105213 FBgn0099217 CPIJ018520 BGIBMGA005798 ACYPI000906 GB13570 AGAP004467 AAEL011103 PHUM479440 GLEAN_09559
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	4282 mit(1)155, y1/FM7a/Dp(1;2;Y)w+
Kyoto	107667 mit(1)155, y1/FM7a/Dp(1;2;Y)w+
VDRC	v24171 w1118; P{GD13807}v24171 v24172 w1118; P{GD13807}v24172
Genomic Clones ( 1 )
	BACR09H13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 38 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD07771
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03205 AT28519 AT31075 GH10368 GH11701 GM04851 LD05286 LD26034 LD31038 LD37631 LD38744 LP10663 LP15535 RE08586 RE37750 RE41306 RE50960 RE60204 RE71377 RH33748 RH44522 RH45838 RH63875 RH69792 SD08482 SD09731 SD19477 SD19783 SD24966
Other clones	64181 bs54b01 CK01.129B.C6 CK01.130A.H10 Dmel_neb_011305_0071_2708 EK095743 EK100461 EK189927
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004643 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47874
Antibody Information
	polyclonal (Williams et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: mit(1)15 CG9900 (Bayraktaroglu, 2000.4.17)
Additional comments	inter se allelism tests have not been performed.
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cell morphology is aberrant, indicative of a failure in cell cycle/mitosis progression. This phenotype is not seen in Kc167 cells. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Candidate gene for testis length quantitative trait locus. (Macdonald and Goldstein, 1999) mit(1)15 can localise to mini-chromosomes that contain the fully functional centromere and to acentric mini-chromosomes that contain only 225-290kb of sub-telomeric DNA. (Williams et al., 1998) Homologs of mit(1)15 identified by sequence similarity are identified. (Starr et al., 1997) mit(1)15 is required for proper chromosome segregation during both meiotic divisions. Function in chromosome segregation must affect both separation of sister chromatids (during mitosis and meiosis II) and the separation of homologous chromosomes (during meiosis I). (Williams et al., 1996) High levels of meiotic nondisjunction due to delay of chromosome migration to the poles at anaphase. Also there is a high frequency of cytokinesis failure. (Williams et al., 1995) Mutations in mit(1)15 can cause essentially random mitotic segregation of chromosomes. (Williams et al., 1992) Based on map position and phenotype mit(1)15 is thought to be synonymous with abe. (Judd et al., 1972) One of a group of fourteen loci identified as temperature-sensitive lethal mutations (Baker) that exhibit, at semirestrictive temperatures, elevated frequencies of clones of homozygous mwh cells in the wings of surviving mit(1);mwh/+ males and of y clones and y//mwh twin spots in the abdomens of y mit(1); T(1;3)scJ4, y+ mwh/+ males. The presence of large clones is consistent with origin via mitotic exchange, mitotic nondisjunction of both homologues, or mutation; twin spots are not expected to result from somatic mutation; only mitotic nondisjunction produces y Sb+ clones in T(1;3)scJ4, y+ Sb/+. Preponderance of small clones suggests origin via chromosome breakage.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080162 NM_001038739 RefSeq (Proteins) • NP_524901 NP_001033828 DDBJ / EMBL / GenBank • AA950879 AAF45794 AAF45794.1 AAL39976 ABC67171 AI534763 AL121804 AL138972 AW944527 AY069831 CAB65854 CAB72295 CAB76122 X64390 Entrez Gene - A searchable database of RefSeq genes. • 47874 UniProtKB/Swiss-Prot • Q9W4X9 UniProtKB/TrEMBL • Q2PE30 Q8T9C2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RZZ complex, subunit Zw10 (IPR009361)
Linkouts
	BioGRID - A database of protein and genetic interactions • 71022 DroID - A comprehensive database of gene and protein interactions. • FBgn0004643 DRSC - Results from RNAi screens. • FBgn0004643 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004643 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9900-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004643 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47874 InParanoid A subset of ortholog calls from InParanoid. • AAEL011103-PA Aedes aegypti (mosquito) AGAP004467-PA Anopheles gambiae (mosquito) XP_001121600.1 Apis mellifera (honey bee) At2g32900.1 Arabidopsis thaliana (wall cress) 213083 Branchiostoma floridae (lancelet) XP_001891601.1 Brugia malayi (roundworm nematode) BGIBMGA005798-PA Bombyx mori (silk worm) ENSBTAP00000026733 Bos taurus (bovine) CE04434 Caenorhabditis elegans (worm) ENSCAFP00000020211 Canis familiaris (dog) ENSCINP00000018123 Ciona intestinalis (sea squirt) CPIJ018520 Culex pipens (mosquito) ENSCPOP00000004585 Cavia porcellus (guinea pig) ENSCSAVP00000016808 Ciona savignyi (sedentary tunicate) 219679 Capitella spl (polychaete worm) DDB0190842 Dictyostelium discoideum (amoeba) ENSDARP00000020574 Danio rerio (zebra fish) ENSECAP00000009910 Equus caballus (horse) ENSGACP00000027445 Gymnopilus aculeatus (mushroom) ENSGALP00000011993 Gallus gallus (chicken) ENSP00000200135 Homo sapiens (human) ISCW010250-PA Ixodes scapularis (deer tick) 225294 Lottia gigantea (owl limpet) ENSMODP00000017739 Monodelphis domestica (gray opossum) ENSMMUP00000031313 Macaca mulatta (rhesus monkey) ENSMUSP00000034803 Mus musculus (house mouse) XP_001605971.1 Nasonia vitripennis (wasp) ENSOANP00000022557 Ornithorynchus anatinus (platypus) ENSORLP00000018055 Oryzias latipes (japanese killifish) PHUM010164-PA Pediculus humanus (human body louse) PP27469 Pristionchus pacificus (nematode) ENSPPYP00000004454 Pongo pygmaeus (orangutan) 552728 Populus trichocarpa (black cottonwood) ENSPTRP00000007380 Pan troglodytes (chimpanzee) ENSRNOP00000010595 Rattus norvegicus (norway rat) 4882387 Sorghum bicolor (sorghum) XP_001180688.1 Strongylocentrotus purpuratus (purple sea urchin) 51689 Trichoplax adhaerens (metazoa) XP_973305.1 Tribolium castaneum (red flour beetle) ENSTNIP00000010462 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000019635 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000049699 Xenopus tropicalis (frog) YALI0A07689g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47874 modMine - Data generated by the modENCODE project. • FBgn0004643 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB19316 NV14663 LH11835 ISCW010250 FBgn0233802 FBgn0218275 FBgn0203046 FBgn0196054 FBgn0174133 JGI_V11_64073 FBgn0022854 FBgn0157601 FBgn0150667 FBgn0139206 FBgn0125358 FBgn0022892 FBgn0105213 FBgn0099217 CPIJ018520 BGIBMGA005798 ACYPI000906 GB13570 AGAP004467 AAEL011103 PHUM479440 GLEAN_09559
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	abe (Fahmy and Fahmy, 1959, ) CG9900 (Benos, 2000.12.13, Whitfield, 2000.4.13, Przewloka et al., 2007, Maresca and Salmon, 2009, Keleman et al., 2009.8.5) dZW10 (Dobie et al., 1999) EG:BACR7C10.3 (Benos, 2000.2.6, Benos, 2000.2.6, Benos et al., 2001, Benos, 2000.12.13, ) l(1)3Af   l(1)6-99   l(1)zw10 (Cook et al., 1997, Aguade and Langley, 1994, Williams et al., 1995, Miyazaki and Orr-Weaver, 1994, Williams and Goldberg, 1994, Foe et al., 1993, Williams and Goldberg, 1992, Williams et al., 1992, Lin and Wolfner, 1989, Ripoll et al., 1987, Paradi et al., 1983, Garcia-Bellido and Robbins, 1983, Lim and Snyder, 1974, Shannon et al., 1972, ) mit(1)15 (Peng and Karpen, 2009, Neumüller et al., 2011) Mit(1)15 (Finan et al., 2011) mit(l)15 (Macdonald and Goldstein, 1999) zw10 (Karess, 2005, Buffin et al., 2005, Lee and Orr-Weaver, 2003, Basto et al., 2000, Savoian et al., 2000, Brunet and Vernos, 2001, Williams et al., 2002, Scaerou et al., 2001, Shah and Cleveland, 2000, Scaerou et al., 2000, Tyler-Smith and Floridia, 2000, Scaerou et al., 1999, Williams, 1992.2.6, Basu et al., 1999, Herrmann et al., 1998, Basu et al., 1998, Philp, 1998, Starr et al., 1997, Williams et al., 1996, Sunkel and Coelho, 1995, Williams et al., 1995, Wu et al., 1994, Montgomery et al., 1991, Goldberg et al., 1989, Schalet, 1986, Lefevre and Watkins, 1986, Smith et al., 1985, Robbins, 1983, Thierry-Mieg, 1982, Lefevre, 1981, Judd et al., 1972, Cenci et al., 2008, Somma et al., 2008, Williams et al., 2007, Peng and Karpen, 2009, Wainman et al., 2009, Musarò et al., 2008) ZW10 (Williams et al., 2003, Gadde and Heald, 2004, Maiato et al., 2002, Blower et al., 2002, Blower and Karpen, 2001, Gonzalez and Bornens, 2000, Savoian and Rieder, 2000, Tomkiel, 2000, Banks and Heald, 2001, Lopez et al., 2000, Platero et al., 1999, Endow, 1999, Logarinho and Sunkel, 1998, Starr et al., 1998, Clarke, 1998, Williams et al., 1998, Allshire, 1997, Starr et al., 1996, Garcia et al., 2007, Przewloka et al., 2007, Oliveira et al., 2005, Maresca and Salmon, 2009, Reis et al., 2009) Zw10 (Basto et al., 2004, Buffin et al., 2005, Griffis et al., 2007, Bucciarelli et al., 2009, Archambault et al., 2008) ZW-10 (Wojcik et al., 2000, Paradi et al., 1983) zw-10 (Nomura and Kurokawa, 1997, Kramers et al., 1991, Kramers et al., 1983, Liu and Lim, 1975)
Name Synonym	abnormal-eye (Fahmy and Fahmy, 1959, ) mitotic 15   Zeste-White 10 (Williams et al., 2003)
Secondary FlyBase IDs
	FBgn0000016 FBgn0002765 FBgn0029627 FBpp0070425 FBtr0070441
References ( 115 )
Generate a list of	All references relating to this gene ( 115 )
List References by type	Research paper  ( 65 ) Supplementary material  ( 5 ) Review  ( 16 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 11 ) FlyBase analysis  ( 1 ) Conference report  ( 2 ) DNA/RNA sequence record  ( 4 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Book  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 2 )
	Finan et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(14): 5566--5571 From the Cover: Proteomics approach to study the functions of Drosophila myosin VI through identification of multiple cargo-binding proteins. [FBrf0213355] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...