Gene Dmel\N

General Information
Symbol	Dmel\N	Species	D. melanogaster
Name	Notch	Annotation symbol	CG3936
Feature type	protein_coding_gene	FlyBase ID	FBgn0004647
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map	1-3.0
Cytogenetic map	3C7-3C9	Sequence location	X:3,028,905..3,066,295 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Notch is referred to in FlyBase by the symbol N (CG3936, FBgn0004647). It has the cytological map location 3C7-3C9. Its sequence location is X:3028905..3066295. Its molecular function is described as: calcium ion binding; transmembrane receptor activity; receptor activity; transcription activator activity; protein binding; specific transcriptional repressor activity. It is involved in the biological processes described with 90 unique terms, many of which group under: anatomical structure development; sensory organ development; regulation of developmental process; organ morphogenesis; gamete generation; ovarian follicle cell development; cell motility; epithelial cell type specification, open tracheal system; hemocyte differentiation; cell adhesion; memory; cell cycle; regulation of neurogenesis. 637 alleles are reported. The phenotypes of these alleles are annotated with 252 unique terms, many of which group under: adult segment; peripheral nervous system; nervous system; organ system; adult mesothoracic segment; embryonic nervous system; primordium; embryonic neuron; embryonic glial cell; embryonic/larval tracheal system. It has one annotated transcript and one annotated polypeptide.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 3C7-3C9 Limits computationally determined from genome sequence between P{EP}EP1362 and P{EP}dnc^EP1395
Experimentally Determined Cytological Location
Cytogenetic map Notes References 3C-3C (Gibson et al., 1999) 3C7-3C8 (determined by in situ hybridisation) (Vieira et al., 1997) 3C7-3C7 (determined by in situ hybridisation) (Kidd et al., 1986) 3C6-3C8 (determined by in situ hybridisation) (Reddy et al., 1984) 3C7-3C7 (determined by in situ hybridisation) (Artavanis-Tsakonas et al., 1983) 3C5-3C11 (determined by in situ hybridisation) (Perlman, 1983) 3C7-3C7 (determined by in situ hybridisation) (Kidd et al., 1983)
Experimentally Determined Recombination Data
Location	1-3.0 (Neel, 1942) 1-2.7 +/- 0.2 (Fahmy and Fahmy, 1958)
Left of (cM)	rb (Welshons and Welshons, 1986) cho (Dambly-Chaudiere and Leyns, 1992)
Right of (cM)	sc (Dambly-Chaudiere and Leyns, 1992) rst su(fa^swb) (Welshons and Welshons, 1986)
Notes	N^77c17 does not recombine with N^60g11 (Welshons). N^fa-g maps 0.061 units to the right of N^55e11, based on 25/41,200 recombinants. N^nd-3 maps 0.014 units to the right of N^fa-g, based on 8/57,600 recombinants. N^fa-1 maps 0.030 units to the right of N^55e11, based on 24/81,000 recombinants. N^fa-1 maps 0.031 units to the left of N^nd-3, based on 8/26,000 recombinants. No recombinants were obtained between N^fa-1 and N^fa-g, out of 65,400 tested chromosomes. N^55e11 maps 0.084 units from N^264-40, based on 33/78,900 recombinants. N^55e11 maps 0.169 units from N^Co, based on 37/43,700 recombinants. N^55e11 maps 0.180 units from N^60g11, based on 41/45,500 recombinants. (Welshons, 1974) N^l1N-ts1 maps 0.0018 units to the right of N^spl-1 and to the left of N^60g11. N^nd-3 maps 0.06 units to the left of N^spl-1. N^l1N-ts2 maps 0.011 units to the right of N^spl-1. N^Ax-tsl maps 0.013 units to the right of N^spl-1. N^nd-ts70j maps 0.037 units to the right of N^spl-1 (at the extreme right boundary of the N locus). (Shellenbarger and Mohler, 1975)
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: rst- kirre+ N+ (Ruiz-Gomez et al., 2000) In direction of increasing cytology: kirre+ N+ (Strunkelnberg et al., 2001) In direction of increasing cytology: N+ CG18508- Fcp3C- CG3939+ dnc+ (Benos, 1999.12.28) Gene Order (overall orientation not stated) References Overall orientation not stated: kirre+ N+ (Struenkelnberg, 1999.9.15)
Gene Model & Products
Please see the GBrowse view of Dmel\N for information on other features
Comments on Gene Model
	DGC clone LD34134 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) N-RA FBtr0070507 NM_057511 10189 2703
Additional Transcript Data & Comments
Reported size (kB)	10.2 (unknown) (Hartley et al., 1987) 10.5 (northern blot) (Wharton et al., 1985, Artavanis-Tsakonas et al., 1983, Artavanis-Tsakonas et al., 1984) 11.7 (northern blot) (Kidd et al., 1983) 10.4 (sequence analysis) (Kidd et al., 1986)
Comments	Two N probes were used, one which contains sequences that include the "EGF-repeats", corresponding to portions of exons 3, 4 and 5, and one which corresponds to portions of exon 6. Both probes were reported to reveal similar expression patterns. (Hartley et al., 1987)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein N-PA FBpp0070483 288.9 2703 5.04 NP_476859 AAF45848
Additional Polypeptide Data & Comments
Reported size (kD)	2703 (aa) (Kidd et al., 1986, Wharton et al., 1985)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004647
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Aspartic acid and asparagine hydroxylation site (IPR000152) EGF-like, type 3 (IPR000742) Notch region (IPR000800) EGF-like, type 2 (IPR001438) EGF-like calcium-binding (IPR001881) Ankyrin (IPR002110) EGF-like (IPR006209) EGF (IPR006210) Notch (IPR008297) Notch, NOD region (IPR010660) Notch, NODP region (IPR011656) EGF-like region, conserved site (IPR013032) EGF calcium-binding (IPR013091)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141415 AA979518 AA979639 AE003426 AAF45848 AF360581 AAL72168 AF360582 AAL72169 AF360583 AAL72170 AF360584 AAL72171 AF360585 AAL72172 AF360586 AAL72173 AF360587 AAL72174 AF360588 AAL72175 AF360589 AAL72176 AF360590 AAL72177 AF360591 AAL72178 AF360592 AAL72179 AF360593 AAL72180 AF360594 AAL72181 AF360595 AAL72182 AF360596 AAL72183 AF360597 AAL72184 AF360598 AAL72185 AF360599 AAL72186 AF360600 AAL72187 AF360601 AAL72188 AF360602 AAL72189 AF360603 AAL72190 AF360604 AAL72191 AF360605 AAL72192 AF360606 AAL72193 AF360607 AAL72194 AF360608 AAL72195 AF360609 AAL72196 AF360610 AAL72197 AF360611 AAL72198 AF360612 AAL72199 AF360613 AAL72200 AF360614 AAL72201 AF360615 AAL72202 AF360616 AAL72203 AF360617 AAL72204 AF360618 AAL72205 AF360619 AAL72206 AF360620 AAL72207 AF360621 AAL72208 AF360622 AAL72209 AF360623 AAL72210 AF360624 AAL72211 AF360625 AAL72212 AF360626 AAL72213 AF360627 AAL72214 AF360628 AAL72215 AF360629 AAL72216 AF360630 AAL72217 AF361372 AAL72219 AF361373 AAL72220 AF361374 AAL72221 AF361375 AAL72222 AF361376 AAL72223 AF361377 AAL72224 AF361378 AAL72225 AF361379 AAL72226 AF361380 AAL72227 AF361381 AAL72228 AF361382 AAL72229 AF361383 AAL72230 AF361384 AAL72231 AF361385 AAL72232 AF361386 AAL72233 AF361387 AAL72234 AF361388 AAL72235 AF361389 AAL72236 AF361390 AAL72237 AF361391 AAL72238 AF361392 AAL72239 AF361393 AAL72240 AF361394 AAL72241 AF361395 AAL72242 AF361396 AAL72243 AF361397 AAL72244 AF361398 AAL72245 AF361399 AAL72246 AF361400 AAL72247 AF361401 AAL72248 AF361402 AAL72249 AF361403 AAL72250 AF361404 AAL72251 AF361405 AAL72252 AF361406 AAL72253 AF361407 AAL72254 AF361408 AAL72255 AF361409 AAL72256 AF361410 AAL72257 AF361411 AAL72258 AF361412 AAL72259 AF361413 AAL72260 AF361414 AAL72261 AF361415 AAL72262 AF361416 AAL72263 AF361417 AAL72264 AF361418 AAL72265 AF361419 AAL72266 AF361420 AAL72267 AF361421 AAL72268 AI124254 AI296361 AL035436 CAB37610 AL121805 CAB58073 AW943211 BF490726 BF500872 BH011521 BI161901 BI161902 BI567043 BI573694 BI585674 BI587247 BI587265 BI589264 BI621821 BI626457 BI628756 BP551643 BP551644 BP555533 BP555534 CO186724 CO264679 CO265544 CO269315 CO269319 CO269682 CO273586 CO283307 CO300802 CO306220 CO308287 CO329593 CO332622 CO338247 CZ472858 EC059630 EC062224 EC062985 EC065091 EC067354 EC067359 EC072550 EC073477 EC083922 EC085809 EC197346 EC203454 EC207734 EC210223 EC217627 EC217935 EC219994 EC232505 EC234421 EC244836 EC248889 EC255466 EC263475 EY198900 EY198963 K03507 AAA28725 K03508 AAA28725