FlyBase Gene Report: Dmel\fs(1)h

OTHER DNA BINDING DOMAIN TRANSCRIPTION FACTORS -: The Other DNA binding domain transcription factors group is a collection of DNA-binding transcription factors that do not fit into any of the other major domain-based transcription factor groups.

Protein Function (UniProtKB)

Required maternally for proper expression of other homeotic genes involved in pattern formation, such as Ubx.

(UniProt, P13709)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

fs(1)h: female sterile (1) homeotic

Many mutant alleles at this locus are nonconditional lethals, but there are three temperature-sensitive hypomorphic alleles. fs(1)h1 is the weakest of these and many of the phenotypic studies have been carried out on it and on Df(1)C128, which is deficient for the locus. Temperature-sensitive genotypes display two periods of sensitivity to elevated temperature; one is during oogenesis and affects embryonic development and the other is during the pupal stage and affects adult survival. Adult survival of fs(1)h1 homozygotes and fs(1)h1/Df(1)C128 heterozygotes is normal at 20 and 25, but 10% and 0 respectively at 28.5. As mentioned above, the TSP for lethality is the pupal stage. fs(1)h15 shown to be lethal in germ-line clones (Perrimon, Engstrom, and Mahowald, 1984a, Dev. Biol. 105: 404-14). Fertility of fs(1)h homozygotes and fs(1)h/Df(1)C128 temperature sensitive with the former becoming completely sterile at 29 and the latter at 25; temperature-sensitive periods for fs(1)h/Df(1)C128 females during pregastrulation embryogenesis and perhaps late oogenesis. Eggs laid at restrictive temperatures exhibited normal preblastoderm development, although about half the nuclei are haploid; nuclear behavior during blastoderm formation disrupted and development arrested in blastoderm or early gastrulation. Larvae and adults surviving semi-permissive temperatures display homeotic anomalies; dead embryos, and newly hatched larvae frequently have missing thoracic and anterior abdominal segments; surviving adults exhibit a sex ratio skewed in favor of males and frequently show missing halteres and less frequently third legs; effects are more severe in progeny of fs(1)h/Df(1)C128 than homozygous fs(1)h females, in daughters than sons, and at 25 than 23; small areas of homeotic transformations of anterior metathoracic to anterior mesothoracic structures also seen. The maternal effect of fs(1)h interacts synergistically with Ubx130, Df(3R)red, and trx, but not bx3 or pbx.

Summary (Interactive Fly)

double-bromodomain protein - trithorax group gene that cooperates with Zeste in activation - regulates dendrite morphology

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\fs(1)h or the JBrowse view of Dmel\fs(1)h for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

fs(1)h-RA

FBtr0071118

NM_167144

9146

1110

fs(1)h-RB

FBtr0071119

NM_078523

8942

2038

fs(1)h-RC

FBtr0071120

NM_206647

9034

1110

fs(1)h-RD

FBtr0071121

NM_206646

9071

1110

fs(1)h-RE

FBtr0071122

NM_206645

8773

1110

fs(1)h-RF

FBtr0301309

NM_001169228

5081

1110

fs(1)h-RG

FBtr0333307

NM_001272392

9949

2046

fs(1)h-RH

FBtr0333308

NM_001272393

9076

1105

fs(1)h-RI

FBtr0333309

NM_001272394

8716

1115

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

fs(1)h-PA

FBpp0071073

110.6

1110

9.83

NP_727228

AAN09226

fs(1)h-PB

FBpp0071074

205.3

2038

9.75

NP_511078

AAF46312

fs(1)h-PC

FBpp0089297

110.6

1110

9.83

NP_996370

AAS65277

fs(1)h-PD

FBpp0089298

110.6

1110

9.83

NP_996369

AAS65278

fs(1)h-PE

FBpp0089299

110.6

1110

9.83

NP_996368

AAS65279

fs(1)h-PF

FBpp0290524

110.6

1110

9.83

NP_001162699

ACZ95234

fs(1)h-PG

FBpp0305499

206.1

2046

9.83

NP_001259321

AGB95166

fs(1)h-PH

FBpp0305500

110.0

1105

9.86

NP_001259322

AGB95167

fs(1)h-PI

FBpp0305501

111.1

1115

9.88

NP_001259323

AGB95168

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1110 aa isoforms: fs(1)h-PA, fs(1)h-PC, fs(1)h-PD, fs(1)h-PE, fs(1)h-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fs(1)h using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (5 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

dendrite morphogenesis

inferred from mutant phenotype

(Bagley et al., 2014)

negative regulation of transcription, DNA-templated

inferred from mutant phenotype

(Florence and Faller, 2008)

terminal region determination

inferred from mutant phenotype

(Florence and Faller, 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

positive regulation of transcription elongation from RNA polymerase II promoter

inferred from sequence or structural similarity with HGNC:13575

(Bisgrove et al., 2007)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

nucleus

inferred from high throughput direct assay

(Lye et al., 2014)

inferred from direct assay

(Bagley et al., 2014, Bisgrove et al., 2007)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 9 -- 10

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

dendritic arborizing neuron

Comment: short isoform

(Bagley et al., 2014)

Additional Descriptive Data

The short isoform of fs(1)h protein is expressed in all dendritic arborizing neurons.

(Bagley et al., 2014)

Marker for

Subcellular Localization

CV Term

Evidence

References

nucleus

inferred from high throughput direct assay

(Lye et al., 2014)

inferred from direct assay

(Bagley et al., 2014, Bisgrove et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\fs(1)h in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\fs(1)h

View exonic expression by tissue for Dmel\fs(1)h

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004656

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0004656

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2252-RB;CG2252-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2252

Flygut - An atlas of the Drosophila adult midgut

FBgn0004656

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\fs(1)h

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 54 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of fs(1)h

Transgenic constructs containing regulatory region of fs(1)h

Deletions and Duplications ( 14 )

Disrupted in

Df(1)C128

(Florence and Faller, 2008, Zheng et al., 2003, Monastirioti et al., 1996, Shearn, 1989, Digan et al., 1986, Eeken et al., 1985)

Df(1)CXE1

(Florence and Faller, 2008)

Df(1)Desi-S3

(Zheng et al., 2003, Florence and McGinnis, 1998)

Df(1)hl-a

(Zheng et al., 2003)

Df(1)PE

(Florence and Faller, 2008)

Df(1)RA2

(Schalet, 1986)

Duplicated in

Dp(1;2)sn⁺72d

(Florence and Faller, 2008, Florence and McGinnis, 1998, Monastirioti et al., 1996, Eeken et al., 1985)

Not disrupted in

Not duplicated in

Dp(1;3)sn^13a1

(Florence and McGinnis, 1998, Eeken et al., 1985)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | germline clone

fs(1)h^h3

lethal

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

fs(1)h^GD4452

lethal | heat sensitive

fs(1)h¹

lethal | heat sensitive (with fs(1)h¹)

lethal | heat sensitive (with fs(1)h^1033B)

fs(1)h¹

lethal | heat sensitive (with fs(1)h^CXE1)

fs(1)h¹

lethal | maternal effect | heat sensitive

fs(1)h¹

lethal | recessive

lethal | rescuable maternal effect

fs(1)h⁶

lethal - all die before end of embryonic stage

lethal - all die before end of embryonic stage | maternal effect | heat sensitive (with fs(1)h¹)

lethal - all die before end of embryonic stage | maternal effect | heat sensitive (with fs(1)h^PA)

fs(1)h¹

lethal - all die before end of embryonic stage | maternal effect | heat sensitive (with fs(1)h^rnc)

fs(1)h¹

lethal - all die before end of embryonic stage | maternal effect | partially | heat sensitive (with fs(1)h¹)

fs(1)h⁴

lethal - all die before end of embryonic stage | maternal effect | partially | heat sensitive (with fs(1)h⁴)

fs(1)h¹

lethal - all die before end of embryonic stage | recessive

fs(1)h^rnc

lethal - all die before end of embryonic stage | recessive | rescuable maternal effect

fs(1)h⁶

lethal - all die before end of larval stage

fs(1)h¹⁵

lethal - all die before end of larval stage | male

lethal - all die before end of larval stage | recessive

lethal - all die before end of pupal stage | heat sensitive

lethal - all die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

fs(1)h^GD4452

viable

fs(1)h^EY10625

viable, with Scer\GAL4^tin.CΔ4

fs(1)h^GD4452

Sterility

Allele

female sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

fs(1)h^GL00006

female sterile | heat sensitive

female sterile | heat sensitive (with Df(1)C128)

fs(1)h¹

female sterile | heat sensitive (with fs(1)h¹)

female sterile | heat sensitive (with fs(1)h⁴)

fs(1)h¹

female sterile | heat sensitive (with fs(1)h^PA)

fs(1)h¹

female sterile | heat sensitive (with fs(1)h^rnc)

fs(1)h¹

female sterile | maternal effect | recessive | heat sensitive

female sterile | recessive

fs(1)h⁶

fertile

fs(1)h^EY10625

Other Phenotypes

Allele

neuroanatomy defective | first instar larval stage | somatic clone

fs(1)h¹¹¹²

neuroanatomy defective | somatic clone | third instar larval stage

fs(1)h¹¹¹²

neuroanatomy defective | third instar larval stage, with Df(1)C128, Scer\GAL4^19-12

fs(1)h^KK101585

neuroanatomy defective | third instar larval stage, with Scer\GAL4^19-12

neuroanatomy defective | third instar larval stage, with Scer\GAL4^109(2)80

fs(1)h^UAS.EGFP

neurophysiology defective | third instar larval stage, with Scer\GAL4^19-12

fs(1)h^GD4452

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

fs(1)h^GD4452

some die during embryonic stage

some die during embryonic stage | maternal effect | heat sensitive (with fs(1)h¹)

some die during embryonic stage | maternal effect | heat sensitive (with fs(1)h^PA)

fs(1)h¹

some die during embryonic stage | maternal effect | heat sensitive (with fs(1)h^rnc)

fs(1)h¹

some die during embryonic stage | partially | heat sensitive (with fs(1)h¹)

fs(1)h⁴

some die during embryonic stage | partially | heat sensitive (with fs(1)h⁴)

fs(1)h¹

some die during embryonic stage | recessive

fs(1)h^rnc

some die during embryonic stage | rescuable maternal effect

fs(1)h⁶

some die during larval stage

fs(1)h¹⁵

some die during larval stage | male

some die during larval stage | recessive

some die during pupal stage | heat sensitive

touch perception defective | third instar larval stage, with Scer\GAL4^19-12

fs(1)h^GD4452

Phenotype manifest in

Allele

abdominal segment 2 | maternal effect | heat sensitive (with fs(1)h¹)

abdominal segment 2 | maternal effect | heat sensitive (with fs(1)h⁴)

fs(1)h¹

abdominal segment 2 | maternal effect | heat sensitive (with fs(1)h^PA)

fs(1)h¹

abdominal segment 2 | maternal effect | heat sensitive (with fs(1)h^rnc)

fs(1)h¹

cephalopharyngeal skeleton

cephalopharyngeal skeleton | maternal effect | heat sensitive (with fs(1)h¹)

fs(1)h^PA

cephalopharyngeal skeleton | maternal effect | heat sensitive (with fs(1)h^PA)

fs(1)h¹

class I dendritic arborizing neuron | somatic clone | third instar larval stage

fs(1)h¹¹¹²

class II dendritic arborizing neuron | somatic clone | third instar larval stage

fs(1)h¹¹¹²

class III dendritic arborizing neuron | first instar larval stage | somatic clone

fs(1)h¹¹¹²

class III dendritic arborizing neuron | somatic clone | third instar larval stage

fs(1)h¹¹¹²

class III dendritic arborizing neuron | third instar larval stage, with Df(1)C128, Scer\GAL4^19-12

fs(1)h^KK101585

class III dendritic arborizing neuron | third instar larval stage, with Scer\GAL4^19-12

class III dendritic arborizing neuron | third instar larval stage, with Scer\GAL4^109(2)80

fs(1)h^UAS.EGFP

class IV dendritic arborizing neuron | somatic clone | third instar larval stage

fs(1)h¹¹¹²

class IV dendritic arborizing neuron | third instar larval stage, with Scer\GAL4^109(2)80

fs(1)h^UAS.EGFP

dendrite | first instar larval stage | somatic clone

fs(1)h¹¹¹²

dendrite | somatic clone | third instar larval stage

fs(1)h¹¹¹²

dendrite | third instar larval stage, with Df(1)C128, Scer\GAL4^19-12

fs(1)h^KK101585

dendrite | third instar larval stage, with Scer\GAL4^19-12

dendrite | third instar larval stage, with Scer\GAL4^109(2)80

fs(1)h^UAS.EGFP

egg

fs(1)h⁶

embryonic/first instar larval cuticle

embryonic/first instar larval cuticle | maternal effect | heat sensitive (with fs(1)h¹)

fs(1)h⁴

embryonic/first instar larval cuticle | maternal effect | heat sensitive (with fs(1)h⁴)

fs(1)h¹

embryonic head

fs(1)h^rnc

filzkorper | maternal effect | heat sensitive (with fs(1)h¹)

filzkorper | maternal effect | heat sensitive (with fs(1)h^PA)

fs(1)h¹

filzkorper | maternal effect | heat sensitive (with fs(1)h^rnc)

fs(1)h¹

hypostomal sclerite

fs(1)h^rnc

larval head | maternal effect | heat sensitive (with fs(1)h¹)

fs(1)h^rnc

larval head | maternal effect | heat sensitive (with fs(1)h^rnc)

fs(1)h¹

metathoracic segment

metathoracic segment | maternal effect

mouth hooks

fs(1)h^rnc

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\BRD2

9 of 15

Yes

Hsap\BRD3

9 of 15

Yes

Hsap\BRD4

9 of 15

Yes

Hsap\BRDT

9 of 15

Yes

Hsap\BAZ2B

1 of 15

Hsap\CCDC127

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Brd2

9 of 15

Yes

Mmus\Brd3

9 of 15

Yes

Mmus\Brdt

9 of 15

Yes

Mmus\Brd4

8 of 15

Yes

Mmus\4933408B17Rik

1 of 15

Rattus norvegicus (Norway rat) (5)

Rnor\Brd4

7 of 13

Yes

Rnor\Brd2

5 of 13

Yes

Rnor\Brd3

4 of 13

Rnor\Brdt

3 of 13

Rnor\LOC108348112

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\brd2

4 of 12

Yes

Xtro\brd4

4 of 12

Yes

Xtro\brdt

4 of 12

Yes

Xtro\brd3

3 of 12

Danio rerio (Zebrafish) (6)

Drer\brd2a

9 of 15

Yes

Drer\brd3a

9 of 15

Yes

Drer\brdt

8 of 15

Yes

Drer\brd4

7 of 15

Yes

Drer\brd3b

5 of 15

Yes

Drer\brd2b

3 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\bet-1

9 of 15

Yes

Cele\bet-2

5 of 15

Yes

Cele\F13C5.2

4 of 15

Cele\F14H12.6

1 of 15

Yes

Cele\F59F5.7

1 of 15

Yes

Cele\nhr-193

1 of 15

Yes

Cele\T24D5.5

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (12)

Atha\GTE3

4 of 9

Yes

3 of 9

3 of 9

3 of 9

3 of 9

Yes

Atha\GTE4

3 of 9

Yes

Atha\GTE7

3 of 9

Yes

Atha\IMB1

3 of 9

Yes

Atha\NPX1

3 of 9

Yes

Atha\GTE8

2 of 9

Atha\AT5G46550

1 of 9

Yes

Atha\GTE6

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\BDF1

11 of 15

Yes

Scer\BDF2

11 of 15

Yes

Scer\SPT7

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\bdf1

8 of 12

Yes

Spom\SPAC631.02

6 of 12

Yes

Spom\bdc1

1 of 12

Spom\spt7

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902SR )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\fs(1)h

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila pseudoobscura pseudoobscura

Dpse\GA26201

Drosophila persimilis

Dper\GL14811

Drosophila willistoni

Dwil\GK15212

Drosophila mojavensis

Dmoj\GI15407

Drosophila grimshawi

Dgri\GH17935

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502GO )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17022148

Glossina morsitans

Tsetse fly

Gmor\GMOY003902

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03730

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002090

Anopheles darlingi

American malaria mosquito

Adar\ADAC006464

Anopheles gambiae

Malaria mosquito

Agam\AGAP002807

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004311

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03PC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Danaus plexippus

Monarch butterfly

Dple\DPOGS204391-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007823

Apis florea

Little honeybee

Aflo\2147541123

Apis mellifera

Western honey bee

Amel\GB49193

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25781

Bombus terrestris

Buff-tailed bumblebee

Bter\15212755

Bombus terrestris

Buff-tailed bumblebee

Bter\15202906

Linepithema humile

Argentine ant

Lhum\LH13398

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002fff

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG009808

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08090

Tribolium castaneum

Red flour beetle

Tcas\TC030673

Pediculus humanus

Human body louse

Phum\PHUM211000

Rhodnius prolixus

Kissing bug

Rpro\RPRC003784

Cimex lectularius

Bed bug

Clec\CLEC002802

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005684

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_14137

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06G6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003490

Ixodes scapularis

Black-legged tick

Isca\ISCW010077

Stegodyphus mimosarum

African social velvet spider

Smim\KFM59840.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM66110.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g05350

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302185

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01QC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Brd

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_003459gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016632gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Brd2

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000023500

Gallus gallus

Domestic chicken

Ggal\BRDT

Gallus gallus

Domestic chicken

Ggal\BRD3

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000013295

Gallus gallus

Domestic chicken

Ggal\BRD2

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

6 of 10

6 of 10

5 of 10

nej

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

BRDT; bromodomain testis associated

model of spermatogenic failure 21

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

BRD2; bromodomain containing 2

9 of 15

601540

BRD3; bromodomain containing 3

9 of 15

601541

BRD4; bromodomain containing 4

9 of 15

608749

BRDT; bromodomain testis associated

9 of 15

602144

SPERMATOGENIC FAILURE 21; SPGF21

spermatogenic failure 21

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

fs(1)h

Hsap\BRD2

(Bagley et al., 2014)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

fs(1)h - BEAF-32

anti bait coimmunoprecipitation, western blot

(Kellner et al., 2013)

fs(1)h - Cp190

pull down, western blot, anti bait coimmunoprecipitation

(Kellner et al., 2013)

fs(1)h - His3

pull down, anti tag western blot

(Kockmann et al., 2013)

fs(1)h - His4

pull down, anti tag western blot

(Kockmann et al., 2013)

fs(1)h - PIP4K

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

fs(1)h - Pc

pull down, western blot, tandem affinity purification, Identification by mass spectrometry, peptide massfingerprinting

(Kang et al., 2015, Strübbe et al., 2011)

fs(1)h - Psc

anti tag coimmunoprecipitation, molecular weight estimation by staining, cross-linking study, tandem affinity purification, Identification by mass spectrometry, western blot

(Kang et al., 2017)

fs(1)h - Su(z)2

cross-linking study, tandem affinity purification, Identification by mass spectrometry

(Kang et al., 2017)

fs(1)h - Trl

anti bait coimmunoprecipitation, western blot, pull down

(Kellner et al., 2013)

fs(1)h - ash1

ion exchange chromatography, anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Kockmann et al., 2013)

fs(1)h - cnc

anti tag coimmunoprecipitation, western blot

(Chatterjee et al., 2016)

fs(1)h - east

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

fs(1)h - mod(mdg4)

anti bait coimmunoprecipitation, western blot, pull down

(Kellner et al., 2013)

fs(1)h - su(Hw)

anti bait coimmunoprecipitation, western blot, pull down

(Kellner et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Shearn, 1989)

suppressible

(Bagley et al., 2014)

fs(1)h

enhanceable

trx

(Shearn, 1989)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

fs(1)h

(Bagley et al., 2014)

Dfd

enhanceable

fs(1)h

(Florence and McGinnis, 1998)

enhanceable

(Ida et al., 2009)

suppressible

(Gregory et al., 2007)

pbl

enhanceable

fs(1)h

(Gregory et al., 2007)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58193

DroID - A comprehensive database of gene and protein interactions.

FBgn0004656

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31722

MIST (genetic) - An integrated Molecular Interaction Database

fs(1)h

MIST (protein-protein) - An integrated Molecular Interaction Database

fs(1)h

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-22

Cytogenetic map

7D3-7D5

Sequence location

X:8,037,808..8,061,436 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

7D3-7D5

Limits computationally determined from genome sequence between P{EP}sn^EP1217 and P{EP}Ubc-E2H^EP1303

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

7D5-7D6

(Schäfer, 2003.12.4)

7D3-7D4

(determined by in situ hybridisation)

(Peter et al., 2002)

12C-12C

(determined by in situ hybridisation) 7D3--4 (determined by in situ hybridisation)

(Schäfer et al., 1999.12.2)

7D5-7D6

(determined by in situ hybridisation)

(Haynes et al., 1989)

7D4-7D6

(determined by in situ hybridisation)

(Digan et al., 1986)

There is a discrepancy between the in situ localisation (12C) and the molecular localisation based on the flanking sequence (7D5--7D6) for the "l(1)G0495" (fs(1)h^G0495) line. The available data suggests that it is most likely that the molecular localisation (7D5--7D6) is correct and that the in situs were misinterpreted.

(Schäfer, 2003.12.4)

Experimentally Determined Recombination Data

Location

1-22

(FlyBase, 2016)

1-22.2

(Comeron et al., 2012)

(Mohler, 1977)

1-21.65

Left of (cM)

(Mohler, 1977)

(Monastirioti et al., 1996)

Right of (cM)

(Monastirioti et al., 1996)

(Mohler, 1977)

Notes

Stocks and Reagents

Stocks (38)

Bloomington

5285

fs(1)h¹⁸/FM7c

10097

w¹¹¹⁸ P{EP}fs(1)h^EP439

Harvard

d03703

P{XP}fs(1)h^d03703

d04280

P{XP}fs(1)h^d04280

f00220

PBac{WH}fs(1)h^f00220

Kyoto

108151

fs(1)h¹⁸/FM7c

108144

C(1;YL)1, y¹ fs(1)h^rnc/FM7c

108152

sn³ fs(1)h⁴ v¹/FM0

123168

w¹¹¹⁸ P{RS3}fs(1)h^CB-0428-3

VDRC

v108662

P{KK101585}VIE-260B

v51227

w¹¹¹⁸; P{GD4452}v51227

v51305

w¹¹¹⁸; P{GD4452}v51305

More stocks available...

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (106)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD26482

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004656

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004656

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31722

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mazina et al., 2018, Kellner et al., 2013)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: fs(1)h CG2252

(Zhang, 1999.11)

Source for database merge of

Source for merge of: fs(1)h rnc

(Florence et al., 2001)

Source for merge of: fs(1)h l(1)G0093 l(1)G0495

(Peter et al., 2002)

Source for merge of: fs(1)h anon- EST:fe1G2

(FlyBase, 1996-)

Additional comments

Source for merge of fs(1)h anon- EST:fe1G2 was sequence comparison ( date:030707 ).

(FlyBase, 1996-)

Other Comments

fs(1)h is necessary for the induction of dendrite branching caused by ct.

(Bagley et al., 2014)

DNA-protein interactions:GEO_GSE30820.

(Sakoparnig et al., 2012)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, and/or aneuploidy, an increase in the proportion of G2/M phase cells, a decrease in mitotic index, a decrease in cytokinetic index and a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells are seen.

(Bettencourt-Dias et al., 2004)

"7D5--6" was stated as revision.

(Schäfer, 2003.12.4)

Used in an investigation to address the relationship between retrotransposons and retroviruses and the coadaptation of these retroelements to their host genomes. Results indicate retrotransposons are heterogeneous in contrast to retroviruses, suggesting different modes of evolution by slippage-like mechanisms.

(Terzian et al., 1997)

A protein sequence motif called the bromodomain has been found in fs(1)h and brm.

(Haynes et al., 1992)

Fertility of fs(1)h homozygotes and fs(1)h/Df(1)C128 temperature-sensitive with the former becoming completely sterile at 29^oC and the latter at 25^oC; temperature-sensitive periods for fs(1)h/Df(1)C128 females during pregastrulation embryogenesis and perhaps late oogenesis. Eggs laid at restrictive temperatures exhibited normal preblastoderm development, although about half the nuclei are haploid; nuclear behavior during blastoderm formation disrupted and development arrested in blastoderm or early gastrulation. Larvae and adults surviving semi-permissive temperatures display homeotic anomalies; dead embryos and newly hatched larvae frequently have missing thoracic and anterior abdominal segments; surviving adults exhibit a sex ratio skewed in favor of males and frequently show missing halteres and less frequently third legs; effects are more severe in progeny of fs(1)h/Df(1)C128 than homozygous fs(1)h females, in daughters than sons and at 25^oC than 23^oC; small areas of homeotic transformations of anterior metathoracic to anterior mesothoracic structures also seen. The maternal effect of fs(1)h interacts synergistically with Ubx¹³⁰, Df(3R)red1 and trx, but not Ubx^bx-3 or Ubx. Most alleles are nonconditional lethals; fine structure mapping places two nonconditional alleles tested distal to the three tested conditional alleles.

(Lindsley and Zimm, 1992)

Ubx, Kr and eve expression are altered in fs(1)h mutant embryos. Defects in the segmental organisation in fs(1)h-deficient progeny are mediated primarily, but not exclusively, through a restriction in the Kr expression domain.

(Huang and Dawid, 1990)

Immunocytochemical staining demonstrates that fs(1)h is involved in establishment of a correct expression pattern for Kr, eve, en and Ubx.

(Huang and Dawid, 1989)

Interactions with a mutation of the fs(1)h homeotic gene were used to confirm that ash1 and ash2 are members of a functionally related group of genes whose alleles have similar transformation properties to mutations of trx.

(Shearn, 1989)

The temperature sensitive period for lethality is the pupal stage.

(Perrimon et al., 1984)

Origin and Etymology

Discoverer

Etymology

Named "rancor" after a (mythological) animal from (fictitious) Tatooine with a similar phenotype.

(Florence and McGinnis, 1998)

Identification

External Crossreferences and Linkouts ( 92 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31722

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P13709

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004656

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004656

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2252

Flygut - An atlas of the Drosophila adult midgut

FBgn0004656

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31722

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030673

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2252

modMine - A data warehouse for the modENCODE project

FBgn0004656

Linkouts

BioGRID - A database of protein and genetic interactions.

58193

DPiM - Drosophila Protein interaction map

FBgn0004656

DroID - A comprehensive database of gene and protein interactions.

FBgn0004656

DRSC - Results frm RNAi screens

FBgn0004656

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0004656

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2252-RB;CG2252-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0004656

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31722

MIST (genetic) - An integrated Molecular Interaction Database

fs(1)h

MIST (protein-protein) - An integrated Molecular Interaction Database

fs(1)h

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

Brd4

(Haberle et al., 2019)

CG2252

(Haelterman et al., 2014, Christis and Munro, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Ida et al., 2009, Keleman et al., 2009.8.5)

FSH

(Kockmann et al., 2013)

Fs(1)h

(Sakamaki et al., 2017, Kellner et al., 2013, Strübbe et al., 2011)

Fs(1)h-L

(Mourad and Cuvier, 2018, Cubeñas-Potts et al., 2017, Mourad et al., 2017, Li et al., 2015)

Fs(1)h/Brd4

(Mazina et al., 2017)

Fs(1)hL

(Florence and Faller, 2008)

Fs(1)hS

(Florence and Faller, 2008)

Fsh

(Comoglio and Paro, 2014, Jennings, 2013, Sakoparnig et al., 2012)

N72

(Schalet, 1986)

ORE-1

(Maia et al., 2007)

anon-EST:Liang-1.81

anon-EST:fe1G2

clone 1.81

(Liang and Biggin, 1998)

fs(1)1456

(Foe et al., 1993, Perrimon et al., 1986, Mohler and Carroll, 1984)

fs(1)26/26A

fs(1)M16

(Perrimon et al., 1986, Mohler and Carroll, 1984, Mohler, 1977)

fs(1)R10.4

fsh

(Jennings, 2013, Florence et al., 2001, Huang et al., 1999, Papoulas et al., 1998, Lin et al., 1997, Terzian et al., 1997, Denis and Green, 1996, Huang et al., 1996, Huang et al., 1995, Muchardt and Yaniv, 1993, Haynes et al., 1992, Huang and Dawid, 1990, Haynes et al., 1989)

fst(1)h

(Madueno et al., 1995)

l(1)7Da

(Zheng et al., 2003)

l(1)G0093

(Schäfer et al., 1999.12.2)

l(1)G0495

(Schäfer et al., 1999.12.2)

rnc

(Florence and McGinnis, 1998)

Name Synonyms

Female sterile 1 homeotic

(Copf, 2015)

anon-fast-evolving-1G2

female sterile (1) homeotic

(Comoglio and Paro, 2014, Jennings, 2013, Lavagnino et al., 2013, Pool et al., 2012, Florence and Faller, 2008, Chang et al., 2007)

female sterile (1) homoeotic

female sterile 1 homeotic

(Bagley et al., 2014)

female sterile homeotic

(Bisgrove et al., 2007)

rancor

(Florence and McGinnis, 1998)

Secondary FlyBase IDs

FBgn0000809
FBgn0001447
FBgn0025246
FBgn0025884
FBgn0040137
FBgn0040199

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (149)

Report Sections

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