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General Information
Symbol
Dmel\fs(1)h
Species
D. melanogaster
Name
female sterile (1) homeotic
Annotation Symbol
CG2252
Feature Type
FlyBase ID
FBgn0004656
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
fsh, Fs(1)h-L, fs(1)M16, fs(1)1456, l(1)G0495
Key Links
Genomic Location
Cytogenetic map
Sequence location
X:8,037,808..8,061,436 [-]
Recombination map
1-22
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
-
Summaries
Gene Group (FlyBase)
OTHER DNA BINDING DOMAIN TRANSCRIPTION FACTORS -
The Other DNA binding domain transcription factors group is a collection of DNA-binding transcription factors that do not fit into any of the other major domain-based transcription factor groups.
Protein Function (UniProtKB)
Required maternally for proper expression of other homeotic genes involved in pattern formation, such as Ubx.
(UniProt, P13709)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
fs(1)h: female sterile (1) homeotic
Many mutant alleles at this locus are nonconditional lethals, but there are three temperature-sensitive hypomorphic alleles. fs(1)h1 is the weakest of these and many of the phenotypic studies have been carried out on it and on Df(1)C128, which is deficient for the locus. Temperature-sensitive genotypes display two periods of sensitivity to elevated temperature; one is during oogenesis and affects embryonic development and the other is during the pupal stage and affects adult survival. Adult survival of fs(1)h1 homozygotes and fs(1)h1/Df(1)C128 heterozygotes is normal at 20 and 25, but 10% and 0 respectively at 28.5. As mentioned above, the TSP for lethality is the pupal stage. fs(1)h15 shown to be lethal in germ-line clones (Perrimon, Engstrom, and Mahowald, 1984a, Dev. Biol. 105: 404-14). Fertility of fs(1)h homozygotes and fs(1)h/Df(1)C128 temperature sensitive with the former becoming completely sterile at 29 and the latter at 25; temperature-sensitive periods for fs(1)h/Df(1)C128 females during pregastrulation embryogenesis and perhaps late oogenesis. Eggs laid at restrictive temperatures exhibited normal preblastoderm development, although about half the nuclei are haploid; nuclear behavior during blastoderm formation disrupted and development arrested in blastoderm or early gastrulation. Larvae and adults surviving semi-permissive temperatures display homeotic anomalies; dead embryos, and newly hatched larvae frequently have missing thoracic and anterior abdominal segments; surviving adults exhibit a sex ratio skewed in favor of males and frequently show missing halteres and less frequently third legs; effects are more severe in progeny of fs(1)h/Df(1)C128 than homozygous fs(1)h females, in daughters than sons, and at 25 than 23; small areas of homeotic transformations of anterior metathoracic to anterior mesothoracic structures also seen. The maternal effect of fs(1)h interacts synergistically with Ubx130, Df(3R)red, and trx, but not bx3 or pbx.
Summary (Interactive Fly)
double-bromodomain protein - trithorax group gene that cooperates with Zeste in activation - regulates dendrite morphology
Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
5

Please see the GBrowse view of Dmel\fs(1)h or the JBrowse view of Dmel\fs(1)h for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.50
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.56
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071118
9146
1110
FBtr0071119
8942
2038
FBtr0071120
9034
1110
FBtr0071121
9071
1110
FBtr0071122
8773
1110
FBtr0301309
5081
1110
FBtr0333307
9949
2046
FBtr0333308
9076
1105
FBtr0333309
8716
1115
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071073
110.6
1110
9.83
FBpp0071074
205.3
2038
9.75
FBpp0089297
110.6
1110
9.83
FBpp0089298
110.6
1110
9.83
FBpp0089299
110.6
1110
9.83
FBpp0290524
110.6
1110
9.83
FBpp0305499
206.1
2046
9.83
FBpp0305500
110.0
1105
9.86
FBpp0305501
111.1
1115
9.88
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fs(1)h using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (5 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
The short isoform of fs(1)h protein is expressed in all dendritic arborizing neurons.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\fs(1)h in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 54 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of fs(1)h
Transgenic constructs containing regulatory region of fs(1)h
Deletions and Duplications ( 14 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
 
 
9 of 15
Yes
Yes
9 of 15
Yes
Yes
9 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
9 of 15
Yes
Yes
9 of 15
Yes
Yes
8 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
7 of 13
Yes
Yes
5 of 13
No
Yes
4 of 13
No
No
3 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
4 of 12
Yes
Yes
4 of 12
Yes
Yes
4 of 12
Yes
Yes
3 of 12
No
No
Danio rerio (Zebrafish) (6)
9 of 15
Yes
Yes
9 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
5 of 15
No
Yes
3 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (7)
9 of 15
Yes
Yes
5 of 15
No
Yes
4 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (12)
4 of 9
Yes
Yes
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
2 of 9
No
No
1 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
11 of 15
Yes
Yes
11 of 15
Yes
Yes
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (4)
8 of 12
Yes
Yes
6 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902SR )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502GO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03PC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06G6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01QC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
6 of 10
6 of 10
5 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Dmel gene
    Ortholog showing functional complementation
    Supporting References
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-22
    Cytogenetic map
    Sequence location
    X:8,037,808..8,061,436 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    7D3-7D5
    Limits computationally determined from genome sequence between P{EP}snEP1217 and P{EP}Ubc-E2HEP1303
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    7D3-7D4
    (determined by in situ hybridisation)
    12C-12C
    (determined by in situ hybridisation) 7D3--4 (determined by in situ hybridisation)
    7D5-7D6
    (determined by in situ hybridisation)
    7D4-7D6
    (determined by in situ hybridisation)
    There is a discrepancy between the in situ localisation (12C) and the molecular localisation based on the flanking sequence (7D5--7D6) for the "l(1)G0495" (fs(1)hG0495) line. The available data suggests that it is most likely that the molecular localisation (7D5--7D6) is correct and that the in situs were misinterpreted.
    Experimentally Determined Recombination Data
    Location
    Notes
    Stocks and Reagents
    Stocks (38)
    Genomic Clones (23)
    cDNA Clones (106)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: fs(1)h CG2252
    Source for database merge of
    Source for merge of: fs(1)h rnc
    Source for merge of: fs(1)h l(1)G0093 l(1)G0495
    Source for merge of: fs(1)h anon- EST:fe1G2
    Additional comments
    Source for merge of fs(1)h anon- EST:fe1G2 was sequence comparison ( date:030707 ).
    Other Comments
    fs(1)h is necessary for the induction of dendrite branching caused by ct.
    DNA-protein interactions:GEO_GSE30820 .
    When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, and/or aneuploidy, an increase in the proportion of G2/M phase cells, a decrease in mitotic index, a decrease in cytokinetic index and a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells are seen.
    "7D5--6" was stated as revision.
    Used in an investigation to address the relationship between retrotransposons and retroviruses and the coadaptation of these retroelements to their host genomes. Results indicate retrotransposons are heterogeneous in contrast to retroviruses, suggesting different modes of evolution by slippage-like mechanisms.
    A protein sequence motif called the bromodomain has been found in fs(1)h and brm.
    Fertility of fs(1)h homozygotes and fs(1)h/Df(1)C128 temperature-sensitive with the former becoming completely sterile at 29oC and the latter at 25oC; temperature-sensitive periods for fs(1)h/Df(1)C128 females during pregastrulation embryogenesis and perhaps late oogenesis. Eggs laid at restrictive temperatures exhibited normal preblastoderm development, although about half the nuclei are haploid; nuclear behavior during blastoderm formation disrupted and development arrested in blastoderm or early gastrulation. Larvae and adults surviving semi-permissive temperatures display homeotic anomalies; dead embryos and newly hatched larvae frequently have missing thoracic and anterior abdominal segments; surviving adults exhibit a sex ratio skewed in favor of males and frequently show missing halteres and less frequently third legs; effects are more severe in progeny of fs(1)h/Df(1)C128 than homozygous fs(1)h females, in daughters than sons and at 25oC than 23oC; small areas of homeotic transformations of anterior metathoracic to anterior mesothoracic structures also seen. The maternal effect of fs(1)h interacts synergistically with Ubx130, Df(3R)red1 and trx, but not Ubxbx-3 or Ubx. Most alleles are nonconditional lethals; fine structure mapping places two nonconditional alleles tested distal to the three tested conditional alleles.
    Ubx, Kr and eve expression are altered in fs(1)h mutant embryos. Defects in the segmental organisation in fs(1)h-deficient progeny are mediated primarily, but not exclusively, through a restriction in the Kr expression domain.
    Immunocytochemical staining demonstrates that fs(1)h is involved in establishment of a correct expression pattern for Kr, eve, en and Ubx.
    Interactions with a mutation of the fs(1)h homeotic gene were used to confirm that ash1 and ash2 are members of a functionally related group of genes whose alleles have similar transformation properties to mutations of trx.
    The temperature sensitive period for lethality is the pupal stage.
    Origin and Etymology
    Discoverer
    Etymology
    Named "rancor" after a (mythological) animal from (fictitious) Tatooine with a similar phenotype.
    Identification
    External Crossreferences and Linkouts ( 92 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (32)
    Reported As
    Symbol Synonym
    Fs(1)h/Brd4
    anon-EST:Liang-1.81
    anon-EST:fe1G2
    fs(1)26/26A
    fs(1)R10.4
    Name Synonyms
    Female sterile 1 homeotic
    anon-fast-evolving-1G2
    female sterile (1) homoeotic
    female sterile 1 homeotic
    female sterile homeotic
    Secondary FlyBase IDs
    • FBgn0000809
    • FBgn0001447
    • FBgn0025246
    • FBgn0025884
    • FBgn0040137
    • FBgn0040199
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (149)