FlyBase Gene Report: Dmel\sim

(Muralidhar et al., 1993)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

myoblast of embryonic/larval muscle system

(Lewis and Crews, 1994)

somatic mesoderm

(Lewis and Crews, 1994)

(Thomas et al., 1988)

embryonic stage 4 -- 6

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 5

(Kasai et al., 1992)

embryonic stage 5 -- 6

(Kearney et al., 2004)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

(Kearney et al., 2004)

embryonic stage 9 -- 10

(Fisher et al., 2012)

embryonic stage 9 -- 12

(Kearney et al., 2004)

embryonic stage 11 -- 12

foregut primordium

(Fisher et al., 2012)

midline ventral glial cell

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 13 -- 16

midline ventral glial cell

(Fisher et al., 2012)

(Fisher et al., 2012)

(Kearney et al., 2004)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 11

Comment: until 6h AEL. Assay specific to sim transcript sim-RA.

(Freer et al., 2011)

embryonic stage 7 -- 16

first instar larval stage

Comment: until 15h AEL. Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

second instar larval stage

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

pupal stage P6 -- pharate adult stage P8

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

embryonic/larval brain

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

pharate adult stage P9 -- pharate adult stage P15(i)

Comment: 24-48h APF. Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

Comment: 72-96h APF. Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

adult stage

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

adult head

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

adult thorax

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

adult abdomen

Comment: Assay specific to sim transcript sim-RB.

(Freer et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

ventral oblique muscle cell

VUM neuron

(Crews et al., 1988)

(Lewis and Crews, 1994)

(Crews et al., 1988)

myoblast of embryonic/larval muscle system

(Crews et al., 1988)

somatic mesoderm

(Lewis and Crews, 1994)

(Lewis and Crews, 1994)

neuroblast MNB

(Crews et al., 1988)

embryonic stage 9

(Wheeler et al., 2006)

embryonic stage 10 -- 15

embryonic foregut | anterior | ventral

(Page, 2003)

embryonic stage 12

(Bossing et al., 2012)

embryonic stage 17

median neuroblast MNB interneuron

(Wheeler et al., 2006)

H-cell sib

(Wheeler et al., 2006)

VUM interneuron

(Wheeler et al., 2006)

neuroblast MNB

(Wheeler et al., 2006)

(Wheeler et al., 2006)

first instar larval stage -- third instar larval stage

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

embryonic/larval brain | posterior | restricted

Comment: referred to as PSC cluster

(Freer et al., 2011)

embryonic/larval brain | lateral | posterior | restricted

Comment: referred to as PLSC cluster

(Freer et al., 2011)

lamina | restricted

(Freer et al., 2011)

(Freer et al., 2011)

neuron of larval ventral nerve cord

(Freer et al., 2011)

Additional Descriptive Data

sim is expressed in the ventral regions of the embryonic foregut and hindgut.

(Maeda et al., 2007)

Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.

(Wheeler et al., 2006)

sim is first detected in the mesoderm at stage 11 in a cluster of 3-5 cells per hemisegment lying just adjacent to the ventral midline. In late stage 11, a more laterally place cluster is observed. Shortly after their appearance, sim-expressing mesodermal cells migrate laterally away from the midline towards the body wall. By the beginning of germ band retraction, the two cell clusters merge and form a single row of sim-positive cells on either side of the ventral midline. These cells elongate and begin to take on the morphology of muscle syncytia. sim expression begins to fade shortly after muscle cell fusion. Weak staining can be detected later in a subset of ventral oblique muscles.

(Lewis and Crews, 1994)

sim protein is first observed at the end of gastrulation in a strip of cells along the ventral midline. The sim-positive cells extend up into the presumptive head region where they form an annulus around the presumptive anterior midgut invagination. By hour 5, the neuroblasts begin delaminating from the ectodermal epithelium. sim-positive cells delaminate to give rise to the midline cells of the CNS. sim protein is expressed in precursors of both neuronal and non-neuronal cells lying at the midline but is found at different level in different cells. By later stages, expression is low in the median neuroblast and in the VUM neurons and is high in the six midline glial cells.

(Crews et al., 1988)

Marker for

medulla

(Zhang et al., 2016)

(Pearson et al., 2012, Nambu et al., 1990)

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Romero et al., 2008)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Crews et al., 1988)

inferred from direct assay

(Romero et al., 2008, Umetsu et al., 2006)

Expression Deduced from Reporters

Reporter: P{2.8sim/lacZ}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

(Kasai et al., 1992)

embryonic stage 5 -- 6

(Kasai et al., 1998)

embryonic stage 10 -- 13

(Wharton et al., 1994)

Reporter: P{3.7sim/lacZ}

Stage

Tissue/Position (including subcellular localization)

Reference

posterior midgut primordium | restricted

(Golembo et al., 1996)

embryonic stage 8 -- 11

stomodeum | restricted

Comment: midline

(Maeda et al., 2007)

Comment: midline

(Maeda et al., 2007)

proctodeum | restricted

Comment: midline

(Maeda et al., 2007)

embryonic stage 10 -- 15

embryonic foregut | restricted

(Ma et al., 2000)

embryonic stage 11

(Maeda et al., 2007)

embryonic hindgut | restricted

(Maeda et al., 2007)

embryonic/larval foregut | restricted

(Maeda et al., 2007)

proctodeum | restricted

(Maeda et al., 2007)

embryonic stage 13 -- 16

(Maeda et al., 2007)

embryonic/larval midgut | restricted

(Maeda et al., 2007)

embryonic/larval hindgut | restricted

(Maeda et al., 2007)

embryonic stage 16

presumptive embryonic/larval central nervous system | restricted

(Ito et al., 1995)

(Ito et al., 1995)

CNS glial cell | restricted

(Ito et al., 1995)

first instar larval stage

glial cell of embryonic/larval central nervous system

(Ito et al., 1995)

embryonic/larval central nervous system | restricted

(Ito et al., 1995)

Reporter: P{7.1sim-lacZ}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

posterior midgut primordium

Comment: reference states 5 hr AEL

(Muralidhar et al., 1993)

Reporter: P{7.8sim-lacZ}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 8 -- 17

(Nambu et al., 1990)

embryonic stage 10

(Nambu et al., 1990)

stomodeum | restricted

(Nambu et al., 1990)

proctodeum

(Nambu et al., 1990)

embryonic stage 10 -- 17

larval ventral nerve cord | restricted

(Nambu et al., 1990)

embryonic stage 13 -- 17

(Nambu et al., 1990)

Reporter: P{GAL4-sim.3.7}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic midline of ventral nerve cord

(Bloomington Drosophila Stock Center, 2017)

embryonic stage 10 -- 17

larval ventral nerve cord | precursor

(Xiao et al., 1996)

(Xiao et al., 1996)

embryonic stage 11

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 11 -- 12

(Fontana and Crews, 2012)

embryonic stage 13

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 16

(Fontana and Crews, 2012)

embryonic stage 17

Comment: all cells

(Wheeler et al., 2006)

Reporter: P{GAL4-sim.S}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 17

(Scholz et al., 1997, Golembo et al., 1996)

embryonic stage 10 -- 15

embryonic foregut | anterior | ventral

(Page, 2003)

embryonic stage 11

(Yan and Lin, 2007)

embryonic stage 11 -- 13

(Bossing et al., 2012)

embryonic stage 15

(Scholz et al., 1997)

Reporter: P{lacW}sim^S147912

Stage

Tissue/Position (including subcellular localization)

Reference

Comment: ubiquitous expression

(Salzberg et al., 1997)

Reporter: P{sim-GAL4.B2.4}

Stage

Tissue/Position (including subcellular localization)

Reference

(Freer et al., 2011)

embryonic/larval brain | posterior | restricted

Comment: referred to as PSC cluster

(Freer et al., 2011)

lamina | restricted

(Freer et al., 2011)

Reporter: P{sim-GAL4.F1.4}

Stage

Tissue/Position (including subcellular localization)

Reference

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

embryonic/larval brain | lateral | posterior | restricted

Comment: referred to as PLSC cluster

(Freer et al., 2011)

(Freer et al., 2011)

Reporter: P{sim-GAL4.L1.0}

Stage

Tissue/Position (including subcellular localization)

Reference

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

embryonic/larval brain | lateral | posterior | restricted

Comment: referred to as PLSC cluster

(Freer et al., 2011)

(Freer et al., 2011)

Reporter: P{sim-GAL4.M582}

Stage

Tissue/Position (including subcellular localization)

Reference

(Freer et al., 2011)

(Freer et al., 2011)

Reporter: P{sim-GAL4.N494}

Stage

Tissue/Position (including subcellular localization)

Reference

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

embryonic/larval brain | lateral | posterior | restricted

Comment: referred to as PLSC cluster

(Freer et al., 2011)

(Freer et al., 2011)

Reporter: P{sim-GAL4.P261}

Stage

Tissue/Position (including subcellular localization)

Reference

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

embryonic/larval brain | lateral | posterior | restricted

Comment: referred to as PLSC cluster

(Freer et al., 2011)

(Freer et al., 2011)

Reporter: P{sim-GAL4.Q255}

Stage

Tissue/Position (including subcellular localization)

Reference

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

(Freer et al., 2011)

Reporter: P{sim-GFP.A1.0}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic/larval brain | posterior | restricted

Comment: referred to as PSC cluster

(Freer et al., 2011)

embryonic/larval brain | lateral | posterior | restricted

Comment: referred to as PLSC cluster

(Freer et al., 2011)

Reporter: P{sim-lacZ.-2608/-127}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 7 -- 8

View Dmel\sim in GBrowse 2

(Morel et al., 2001)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\sim

View exonic expression by tissue for Dmel\sim

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004666

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41612/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004666

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004666

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004666

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7771

Flygut - An atlas of the Drosophila adult midgut

FBgn0004666

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\sim

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 36 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 26 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of sim

Transgenic constructs containing regulatory region of sim

Aberrations (Deficiencies and Duplications) ( 21 )

Inferred from experimentation ( 21 )

Gene disrupted in

Df(3R)Exel6167

(Ryder, 2004.4.26)

Df(3R)kar-Sz11

(Rushlow and Chovnick, 1984, Hilliker et al., 1981, Hilliker et al., 1980)

Df(3R)kar-Sz28

(Deak et al., 1997, Salzberg et al., 1997)

Df(3R)l26d

(Nambu et al., 1990, DeLotto and Spierer, 1986, Bossy et al., 1984, Rushlow and Chovnick, 1984, Hall et al., 1983, Spierer et al., 1983, Hilliker et al., 1981, Hilliker et al., 1980)

Df(3R)ry27

(Harbecke and Lengyel, 1995)

Df(3R)ry75

(Pielage et al., 2002)

Df(3R)ry81

(Bossy et al., 1984, Hall et al., 1983, Spierer et al., 1983)

Df(3R)ry506-85C

(Harbecke and Lengyel, 1995)

Df(3R)ry615

(Harbecke and Lengyel, 1995)

Df(3R)ry619

(Nambu et al., 1990, Manseau et al., 1988, Thomas et al., 1988, Rushlow and Chovnick, 1984, Hilliker et al., 1981, Hilliker et al., 1980)

Df(3R)ry1168

(Manseau et al., 1988, Rushlow and Chovnick, 1984, Hilliker et al., 1981, Hilliker et al., 1980)

Df(3R)ry1301

(Rushlow and Chovnick, 1984, Hilliker et al., 1981, Hilliker et al., 1980)

Df(3R)ry1402

(Bossy et al., 1984, Rushlow and Chovnick, 1984, Hall et al., 1983, Spierer et al., 1983, Hilliker et al., 1981, Hilliker et al., 1980)

Df(3R)ry1607

(Rushlow and Chovnick, 1984, Hilliker et al., 1981, Hilliker et al., 1980)

Df(3R)ry1608

(Bossy et al., 1984, Rushlow and Chovnick, 1984, Hall et al., 1983, Spierer et al., 1983, Hilliker et al., 1981, Hilliker et al., 1980)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal (with Df(3R)kar-Sz28)

lethal | heat sensitive (with sim²)

sim^J1-47

lethal | heat sensitive (with sim^J1-47)

sim²

lethal | recessive

lethal - all die during embryonic stage

lethal - all die during embryonic stage | recessive

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

sim^GD13195

viable, with Scer\GAL4^Mef2.PR

sim^GD13195

viable, with Scer\GAL4^pnr-MD237

sim^GD13195

viable, with Scer\GAL4^tin.CΔ4

sim^GD13195

viable (with sim²)

viable (with sim^ry75)

sim²

viable (with sim^ry614)

sim²

Sterility

Allele

female sterile | recessive

sim^J1-47

fertile

sim^f05216

male sterile | recessive

sim^J1-47

sterile (with sim²)

sterile (with sim^J1-47)

sim²

sterile (with sim^ry75)

sim²

sterile (with sim^ry614)

sim²

Other Phenotypes

Allele

abnormal cell migration | embryonic stage

abnormal courtship behavior (with sim²)

sim^J1-47

abnormal courtship behavior (with sim^J1-47)

sim²

abnormal locomotor behavior (with sim²)

sim^J1-47

abnormal locomotor behavior (with sim^J1-47)

sim²

abnormal neuroanatomy

abnormal neuroanatomy, with Scer\GAL4^NP6099

sim^UAS.cCa

abnormal neuroanatomy, with Scer\GAL4^sim.P3.7

sim^UAS.cXa

abnormal neuroanatomy (with sim²)

abnormal neuroanatomy (with sim^J1-47)

sim²

abnormal neuroanatomy (with sim^ry75)

sim²

abnormal neuroanatomy | dorsal closure stage

sim²

abnormal neuroanatomy | embryonic stage

sim²

abnormal neuroanatomy | heat sensitive

sim^J1-47

abnormal neuroanatomy | somatic clone | third instar larval stage

abnormal optomotor response (with sim²)

sim^J1-47

abnormal optomotor response (with sim^J1-47)

sim²

decreased cell number | dorsal closure stage

sim²

decreased occurrence of cell division | embryonic stage

sim²

increased cell death | embryonic stage

sim²

increased cell death | partially

sim²

pair rule phenotype, with Scer\GAL4^prd.RG1

sim^UAS.cXa

some die during embryonic stage

some die during embryonic stage | recessive

visible, with Scer\GAL4^Bx-MS1096

sim^{UAS.ORF.GW.Tag:HA}

visible, with Scer\GAL4^ey.PH

sim^UAS.cXa

visible, with Scer\GAL4^pnr-MD237

sim^GD13195

visible (with sim²)

sim^J1-47

visible (with sim^J1-47)

sim²

visible | cold sensitive, with Scer\GAL4^Bx-MS1096

sim^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

abdominal 1 dorsal VUM motor neuron

sim²

abdominal 1 lateral VUM motor neuron

sim²

abdominal 1 ventral VUM motor neuron

sim²

abdominal 2 dorsal VUM motor neuron

sim²

abdominal 2 lateral VUM motor neuron

sim²

abdominal 2 ventral VUM motor neuron

sim²

abdominal 3 dorsal VUM motor neuron

sim²

abdominal 3 lateral VUM motor neuron

sim²

abdominal 3 ventral VUM motor neuron

sim²

abdominal 4 dorsal VUM motor neuron

sim²

abdominal 4 lateral VUM motor neuron

sim²

abdominal 4 ventral VUM motor neuron

sim²

abdominal 5 dorsal VUM motor neuron

sim²

abdominal 5 lateral VUM motor neuron

sim²

abdominal 5 ventral VUM motor neuron

sim²

abdominal 6 dorsal VUM motor neuron

sim²

abdominal 6 lateral VUM motor neuron

sim²

abdominal 6 ventral VUM motor neuron

sim²

abdominal 7 dorsal VUM motor neuron

sim²

abdominal 7 lateral VUM motor neuron

sim²

abdominal 7 ventral VUM motor neuron

sim²

adult central complex (with sim²)

sim^J1-47

adult central complex (with sim^J1-47)

sim²

adult head, with Scer\GAL4^ey.PH

sim^UAS.cXa

adult hindgut

sim^J1-47

aedeagus

sim^J1-47

analia (with sim²)

sim^J1-47

analia (with sim^J1-47)

sim²

anal slit

sim²

anal slit | heat sensitive

sim^J1-47

anal slit | heat sensitive (with sim²)

sim^J1-47

anal slit | heat sensitive (with sim^J1-47)

sim²

antenna, with Scer\GAL4^ey.PH

sim^UAS.cXa

axon

sim^unspecified

axon | somatic clone | third instar larval stage

cell cycle

sim²

central nervous system

cephalopharyngeal skeleton

sim^unspecified

cercus

dendritic arborizing neuron

sim^unspecified

denticle belt

dorsal neurohemal organ

sim²

embryonic/larval anal pad

sim²

embryonic/larval brain (with sim²)

sim^ry75

embryonic/larval brain (with sim^ry75)

sim²

embryonic/larval cuticle, with Scer\GAL4^prd.RG1

sim^UAS.cXa

embryonic/larval plasmatocyte

sim⁸

embryonic brain, with Scer\GAL4^sim.P3.7

sim^UAS.cXa

embryonic central brain

sim²

embryonic epidermis

sim^hs.PN

embryonic head

sim^hs.PN

embryonic neuroblast

sim²

embryonic primordium of adult salivary gland

sim²

embryonic segment, with Scer\GAL4^prd.RG1

sim^UAS.cXa

embryonic somatic muscle cell | ventral

embryonic ventral epidermis

exit glial cell | embryonic stage 15

sim²

eye, with Scer\GAL4^ey.PH

sim^UAS.cXa

eye photoreceptor cell & axon (with sim²)

sim^ry75

eye photoreceptor cell & axon (with sim^ry75)

sim²

fan-shaped body (with sim²)

sim^J1-47

fan-shaped body (with sim^J1-47)

sim²

female analia

sim^J1-47

ganglion mother cell GMC1-1a

genital disc primordium

genitalia (with sim²)

sim^J1-47

genitalia (with sim^J1-47)

sim²

gonopod thorn bristle

sim^J1-47

lamina forming neuroblast, with Scer\GAL4^NP6099

sim^UAS.cCa

lamina forming neuroblast (with sim²)

sim^ry75

lamina forming neuroblast (with sim^ry75)

sim²

larval longitudinal connective

larval longitudinal connective | heat sensitive

sim^J1-47

larval midline neuron

sim¹

larval ventral nerve cord | embryonic stage

sim²

larval ventral nerve cord commissure (with sim²)

sim^J1-47

larval ventral nerve cord commissure (with sim^J1-47)

sim²

larval ventral nerve cord commissure | heat sensitive

sim^J1-47

lobula (with sim²)

sim^J1-47

lobula (with sim^J1-47)

sim²

medial cell body glial cell | embryonic stage 13

sim²

medial-most cell body glial cell | embryonic stage 13

sim²

midline glial cell | dorsal closure stage

sim²

midline glial cell | ectopic, with Scer\GAL4^sca-537.4

sim^UAS.cMa

midline glial cell | embryonic stage 13

sim²

midline neuronal primordium | embryonic stage

MP1 neuron

MP1 precursor

muscle founder cell

sim²

neuroblast MNB

sim²

neuroglioblast | embryonic stage 12

sim²

neuromere

sim²

neuron, with Scer\GAL4^en-e16E

sim^UAS.cMa

optic chiasma (with sim²)

sim^J1-47

optic chiasma (with sim^J1-47)

sim²

ovary

sim^J1-47

peripheral glial cell | embryonic stage

sim²

peripheral glial cell | embryonic stage 14

sim²

peripheral glial cell | embryonic stage 16

sim²

presumptive embryonic/larval central nervous system

presumptive embryonic/larval central nervous system (with sim²)

sim^J1-47

presumptive embryonic/larval central nervous system (with sim^J1-47)

sim²

protocerebral bridge (with sim²)

sim^J1-47

protocerebral bridge (with sim^J1-47)

sim²

RP2 motor neuron | ectopic

RP2sib neuron

surstylus

sim^J1-47

symmetrical commissure

testis

sim^J1-47

trichogen cell, with Scer\GAL4^pnr-MD237

sim^GD13195

ventral arm of pharyngeal sclerite

sim¹

ventral midline of embryo | embryonic stage

sim⁹

ventral nerve cord

sim¹

ventral nerve cord primordium & mitotic cell cycle

sim²

vulva

sim^J1-47

VUM4 interneuron

sim⁵

VUM5 interneuron

sim⁵

VUM6 interneuron

sim⁵

VUM neuron

sim⁵

VUM precursor

wing, with Scer\GAL4^Bx-MS1096

sim^{UAS.ORF.GW.Tag:HA}

wing | cold sensitive, with Scer\GAL4^Bx-MS1096

sim^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SIM1

9 of 15

Yes

Hsap\SIM2

9 of 15

Yes

5 of 15

3 of 15

2 of 15

2 of 15

2 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Sim1

9 of 15

Yes

Mmus\Sim2

9 of 15

Yes

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Rattus norvegicus (Norway rat) (8)

Rnor\Sim1

8 of 13

Yes

Rnor\Sim2

5 of 13

Yes

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (6)

Xtro\sim2

7 of 12

Yes

Xtro\sim1

5 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (11)

Drer\sim1a

9 of 15

Yes

Drer\sim1b

9 of 15

Yes

Drer\sim2

5 of 15

Yes

4 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\hlh-34

6 of 15

Yes

Cele\hif-1

3 of 15

Cele\cky-1

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091906BQ )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA26599

Drosophila persimilis

Dper\GL24392

Drosophila willistoni

Dwil\GK22407

Drosophila virilis

Dvir\sim

Drosophila mojavensis

Dmoj\GI23369

Drosophila grimshawi

Dgri\GH18379

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504IF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17014016

Glossina morsitans

Tsetse fly

Gmor\GMOY004224

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07881

Mayetiola destructor

Hessian fly

Mdes\Mdes005255

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011013

Anopheles darlingi

American malaria mosquito

Adar\ADAC005401

Anopheles darlingi

American malaria mosquito

Adar\ADAC005208

Anopheles gambiae

Malaria mosquito

Agam\AGAP000773

Anopheles gambiae

Malaria mosquito

Agam\AGAP010058

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009948

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04BH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA003821

Danaus plexippus

Monarch butterfly

Dple\DPOGS203878-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS203877-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL008134

Apis florea

Little honeybee

Aflo\4512044196

Apis mellifera

Western honey bee

Amel\GB51615

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP16878

Bombus terrestris

Buff-tailed bumblebee

Bter\15212112

Linepithema humile

Argentine ant

Lhum\LH25113

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0015d3

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG014230

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07687

Tribolium castaneum

Red flour beetle

Tcas\TC016205

Pediculus humanus

Human body louse

Phum\PHUM080130

Rhodnius prolixus

Kissing bug

Rpro\sim

Cimex lectularius

Bed bug

Clec\CLEC009551

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007240

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_06765

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0484 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR001482

Ixodes scapularis

Black-legged tick

Isca\ISCW021887

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77734.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g06010

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_311851

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06FQ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Sim

Gallus gallus

Domestic chicken

Ggal\SIM1

Gallus gallus

Domestic chicken

Ggal\SIM2

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (3)

Gene Symbol

Score

Source

Alignment

4 of 10

trh

4 of 10

dysf

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SIM1; SIM bHLH transcription factor 1

9 of 15

603128

SIM2; SIM bHLH transcription factor 2

9 of 15

600892

NPAS1; neuronal PAS domain protein 1

5 of 15

603346

NPAS3; neuronal PAS domain protein 3

3 of 15

609430

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

sim - Cdk7

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

sim - CycH

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

sim - D

pull down, autoradiography, two hybrid

(Ma et al., 2000)

sim - Hinfp

two hybrid array

(Shokri et al., 2019)

sim - Hr78

two hybrid array

(Shokri et al., 2019)

sim - tgo

two hybrid, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, two hybrid array, anti bait coimmunoprecipitation, western blot

(Shokri et al., 2019, Rhee et al., 2014, Guruharsha et al., 2011, Emmons et al., 1999, Sonnenfeld et al., 1997)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

CycE

(Jiao et al., 2001)

suppressible

(Page, 2003)

enhanceable

(Sedaghat et al., 2002)

suppressible

Myc

(Jiao et al., 2001)

enhanceable

sli

(Sedaghat et al., 2002)

sim|jing

enhanceable

tgo

(Sedaghat et al., 2002)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Ma et al., 2000)

jing

enhanceable

(Sedaghat et al., 2002)

tgo

suppressible