Gene Dmel\Mlc-c

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

EF-Hand 1, calcium-binding site (IPR018247)

EF-hand domain (IPR002048)

EF-hand-like domain (IPR011992)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P54357

UniProtKB/TrEMBL

Q95SW7

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

myosin II complex

inferred from physical interaction with zip

myosin V complex

inferred from physical interaction with didum

myosin VI complex

inferred from physical interaction with jar

myosin VII complex

inferred from physical interaction with ck

(The modENCODE Consortium, 2010, Graveley et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1481.825
Larval Midgut		3929.6
Larval Hindgut		2964.7
Larval Malpighian Tubules		3560.2
Larval Fat Body		1806.8
Larval Salivary Gland		6098.7
Larval Trachea		2995.75
Larval Carcass		2060.6
Adult Head		1040.1
Adult Eye		1925.375
Adult Brain		596.8
Adult Thoracic-Abdominal Ganglion		559.3
Adult Crop		2943.8
Adult Midgut		3409.7
Adult Hindgut		2031.4
Adult Malpighian Tubules		3336.4
Adult Fat Body		990.4
Adult Salivary Gland		3706.8
Adult Heart		1294.725
Adult VirginFemale Spermatheca		1996.4
Adult InseminatedFemale Spermatheca		2086.4
Adult Ovary		1354.7
Adult Testis		776.6
Adult Male Accessory Gland		1781.7
Adult Carcass		740.7
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1481.825)
Larval Midgut		(3929.6)
Larval Hindgut		(2964.7)
Larval Malpighian Tubules		(3560.2)
Larval Fat Body		(1806.8)
Larval Salivary Gland		(6098.7)
Larval Trachea		(2995.75)
Larval Carcass		(2060.6)
Adult Head		(1040.1)
Adult Eye		(1925.375)
Adult Brain		(596.8)
Adult Thoracic-Abdominal Ganglion		(559.3)
Adult Crop		(2943.8)
Adult Midgut		(3409.7)
Adult Hindgut		(2031.4)
Adult Malpighian Tubules		(3336.4)
Adult Fat Body		(990.4)
Adult Salivary Gland		(3706.8)
Adult Heart		(1294.725)
Adult VirginFemale Spermatheca		(1996.4)
Adult InseminatedFemale Spermatheca		(2086.4)
Adult Ovary		(1354.7)
Adult Testis		(776.6)
Adult Male Accessory Gland		(1781.7)
Adult Carcass		(740.7)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(1481.825)
Larval Midgut		(3929.6)
Larval Hindgut		(2964.7)
Larval Malpighian Tubules		(3560.2)
Larval Fat Body		(1806.8)
Larval Salivary Gland		(6098.7)
Larval Trachea		(2995.75)
Larval Carcass		(2060.6)
Adult Head		1040.1
Adult Eye		(1925.375)
Adult Brain		596.8
Adult Thoracic-Abdominal Ganglion		559.3
Adult Crop		(2943.8)
Adult Midgut		(3409.7)
Adult Hindgut		(2031.4)
Adult Malpighian Tubules		(3336.4)
Adult Fat Body		990.4
Adult Salivary Gland		(3706.8)
Adult Heart		(1294.725)
Adult VirginFemale Spermatheca		(1996.4)
Adult InseminatedFemale Spermatheca		(2086.4)
Adult Ovary		(1354.7)
Adult Testis		776.6
Adult Male Accessory Gland		(1781.7)
Adult Carcass		740.7
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1481.825
Larval Midgut		3929.6
Larval Hindgut		2964.7
Larval Malpighian Tubules		3560.2
Larval Fat Body		1806.8
Larval Salivary Gland		6098.7
Larval Trachea		2995.75
Larval Carcass		2060.6
Adult Head		1040.1
Adult Eye		1925.375
Adult Brain		596.8
Adult Thoracic-Abdominal Ganglion		559.3
Adult Crop		2943.8
Adult Midgut		3409.7
Adult Hindgut		2031.4
Adult Malpighian Tubules		3336.4
Adult Fat Body		990.4
Adult Salivary Gland		3706.8
Adult Heart		1294.725
Adult VirginFemale Spermatheca		1996.4
Adult InseminatedFemale Spermatheca		2086.4
Adult Ovary		1354.7
Adult Testis		776.6
Adult Male Accessory Gland		1781.7
Adult Carcass		740.7
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1481.825
Larval Midgut		3929.6
Larval Hindgut		2964.7
Larval Malpighian Tubules		3560.2
Larval Fat Body		1806.8
Larval Salivary Gland		6098.7
Larval Trachea		2995.75
Larval Carcass		2060.6
Adult Head		1040.1
Adult Eye		1925.375
Adult Brain		596.8
Adult Thoracic-Abdominal Ganglion		559.3
Adult Crop		2943.8
Adult Midgut		3409.7
Adult Hindgut		2031.4
Adult Malpighian Tubules		3336.4
Adult Fat Body		990.4
Adult Salivary Gland		3706.8
Adult Heart		1294.725
Adult VirginFemale Spermatheca		1996.4
Adult InseminatedFemale Spermatheca		2086.4
Adult Ovary		1354.7
Adult Testis		776.6
Adult Male Accessory Gland		1781.7
Adult Carcass		740.7
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1481.825)
Larval Midgut		(3929.6)
Larval Hindgut		(2964.7)
Larval Malpighian Tubules		(3560.2)
Larval Fat Body		(1806.8)
Larval Salivary Gland		(6098.7)
Larval Trachea		(2995.75)
Larval Carcass		(2060.6)
Adult Head		(1040.1)
Adult Eye		(1925.375)
Adult Brain		596.8
Adult Thoracic-Abdominal Ganglion		559.3
Adult Crop		(2943.8)
Adult Midgut		(3409.7)
Adult Hindgut		(2031.4)
Adult Malpighian Tubules		(3336.4)
Adult Fat Body		(990.4)
Adult Salivary Gland		(3706.8)
Adult Heart		(1294.725)
Adult VirginFemale Spermatheca		(1996.4)
Adult InseminatedFemale Spermatheca		(2086.4)
Adult Ovary		(1354.7)
Adult Testis		776.6
Adult Male Accessory Gland		(1781.7)
Adult Carcass		740.7
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1481.825
Larval Midgut		(3929.6)
Larval Hindgut		(2964.7)
Larval Malpighian Tubules		(3560.2)
Larval Fat Body		(1806.8)
Larval Salivary Gland		(6098.7)
Larval Trachea		(2995.75)
Larval Carcass		(2060.6)
Adult Head		1040.1
Adult Eye		(1925.375)
Adult Brain		596.8
Adult Thoracic-Abdominal Ganglion		559.3
Adult Crop		(2943.8)
Adult Midgut		(3409.7)
Adult Hindgut		(2031.4)
Adult Malpighian Tubules		(3336.4)
Adult Fat Body		990.4
Adult Salivary Gland		(3706.8)
Adult Heart		1294.725
Adult VirginFemale Spermatheca		(1996.4)
Adult InseminatedFemale Spermatheca		(2086.4)
Adult Ovary		1354.7
Adult Testis		776.6
Adult Male Accessory Gland		(1781.7)
Adult Carcass		740.7
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1481.825
Larval Midgut		3929.6
Larval Hindgut		2964.7
Larval Malpighian Tubules		3560.2
Larval Fat Body		1806.8
Larval Salivary Gland		6098.7
Larval Trachea		2995.75
Larval Carcass		2060.6
Adult Head		1040.1
Adult Eye		1925.375
Adult Brain		596.8
Adult Thoracic-Abdominal Ganglion		559.3
Adult Crop		2943.8
Adult Midgut		3409.7
Adult Hindgut		2031.4
Adult Malpighian Tubules		3336.4
Adult Fat Body		990.4
Adult Salivary Gland		3706.8
Adult Heart		1294.725
Adult VirginFemale Spermatheca		1996.4
Adult InseminatedFemale Spermatheca		2086.4
Adult Ovary		1354.7
Adult Testis		776.6
Adult Male Accessory Gland		1781.7
Adult Carcass		740.7
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	1040.1
NA	Eye	1925.375
NA	Brain	596.8
1481.825	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	559.3
NA	Crop	2943.8
3929.6	Midgut	3409.7
2964.7	Hindgut	2031.4
3560.2	Malpighian Tubules	3336.4
1806.8	Fat Body	990.4
6098.7	Salivary Gland	3706.8
NA	Heart	1294.725
2995.75	Trachea	NA
NA	VirginFemale Spermatheca	1996.4
NA	InseminatedFemale Spermatheca	2086.4
NA	Ovary	1354.7
NA	Testis	776.6
NA	Male Accessory Gland	1781.7
2060.6	Carcass	740.7

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		119
central nervous system, larvae L3		95
central nervous system, pupae P8		64
head, virgin 1-day female		46
head, virgin 4-day female		46
head, virgin 20-day female		33
head, mated 1-day female		47
head, mated 4-day female		39
head, mated 20-day female		28
head, mated 1-day male		59
head, mated 4-day male		50
head, mated 20-day male		48
salivary gland, larvae L3 wandering		197
salivary gland, white prepupae		366
digestive system, larvae L3 wandering		298
digestive system, 1-day adult		246
digestive system, 4-day adult		205
digestive system, 20-day adult		187
fat body, larvae L3 wandering		22
fat body, white prepupae		77
fat body, pupae P8		85
carcass, larvae L3 wandering		132
carcass, 1-day adult		32
carcass, 4-day adult		36
carcass, 20-day adult		41
ovary, virgin 4-day female		75
ovary, mated 4-day female		69
testis, mated 4-day male		63
accessory gland, mated 4-day male		66
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(119)
central nervous system, larvae L3		(95)
central nervous system, pupae P8		(64)
head, virgin 1-day female		(46)
head, virgin 4-day female		(46)
head, virgin 20-day female		(33)
head, mated 1-day female		(47)
head, mated 4-day female		(39)
head, mated 20-day female		(28)
head, mated 1-day male		(59)
head, mated 4-day male		(50)
head, mated 20-day male		(48)
salivary gland, larvae L3 wandering		(197)
salivary gland, white prepupae		(366)
digestive system, larvae L3 wandering		(298)
digestive system, 1-day adult		(246)
digestive system, 4-day adult		(205)
digestive system, 20-day adult		(187)
fat body, larvae L3 wandering		22
fat body, white prepupae		(77)
fat body, pupae P8		(85)
carcass, larvae L3 wandering		(132)
carcass, 1-day adult		(32)
carcass, 4-day adult		(36)
carcass, 20-day adult		(41)
ovary, virgin 4-day female		(75)
ovary, mated 4-day female		(69)
testis, mated 4-day male		(63)
accessory gland, mated 4-day male		(66)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(119)
central nervous system, larvae L3		95
central nervous system, pupae P8		64
head, virgin 1-day female		46
head, virgin 4-day female		46
head, virgin 20-day female		33
head, mated 1-day female		47
head, mated 4-day female		39
head, mated 20-day female		28
head, mated 1-day male		59
head, mated 4-day male		50
head, mated 20-day male		48
salivary gland, larvae L3 wandering		(197)
salivary gland, white prepupae		(366)
digestive system, larvae L3 wandering		(298)
digestive system, 1-day adult		(246)
digestive system, 4-day adult		(205)
digestive system, 20-day adult		(187)
fat body, larvae L3 wandering		22
fat body, white prepupae		77
fat body, pupae P8		85
carcass, larvae L3 wandering		(132)
carcass, 1-day adult		32
carcass, 4-day adult		36
carcass, 20-day adult		41
ovary, virgin 4-day female		75
ovary, mated 4-day female		69
testis, mated 4-day male		63
accessory gland, mated 4-day male		66
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		119
central nervous system, larvae L3		95
central nervous system, pupae P8		64
head, virgin 1-day female		46
head, virgin 4-day female		46
head, virgin 20-day female		33
head, mated 1-day female		47
head, mated 4-day female		39
head, mated 20-day female		28
head, mated 1-day male		59
head, mated 4-day male		50
head, mated 20-day male		48
salivary gland, larvae L3 wandering		197
salivary gland, white prepupae		366
digestive system, larvae L3 wandering		298
digestive system, 1-day adult		246
digestive system, 4-day adult		205
digestive system, 20-day adult		187
fat body, larvae L3 wandering		22
fat body, white prepupae		77
fat body, pupae P8		85
carcass, larvae L3 wandering		132
carcass, 1-day adult		32
carcass, 4-day adult		36
carcass, 20-day adult		41
ovary, virgin 4-day female		75
ovary, mated 4-day female		69
testis, mated 4-day male		63
accessory gland, mated 4-day male		66
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		119
central nervous system, larvae L3		95
central nervous system, pupae P8		64
head, virgin 1-day female		46
head, virgin 4-day female		46
head, virgin 20-day female		33
head, mated 1-day female		47
head, mated 4-day female		39
head, mated 20-day female		28
head, mated 1-day male		59
head, mated 4-day male		50
head, mated 20-day male		48
salivary gland, larvae L3 wandering		197
salivary gland, white prepupae		366
digestive system, larvae L3 wandering		298
digestive system, 1-day adult		246
digestive system, 4-day adult		205
digestive system, 20-day adult		187
fat body, larvae L3 wandering		22
fat body, white prepupae		77
fat body, pupae P8		85
carcass, larvae L3 wandering		132
carcass, 1-day adult		32
carcass, 4-day adult		36
carcass, 20-day adult		41
ovary, virgin 4-day female		75
ovary, mated 4-day female		69
testis, mated 4-day male		63
accessory gland, mated 4-day male		66
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(119)
central nervous system, larvae L3		(95)
central nervous system, pupae P8		(64)
head, virgin 1-day female		(46)
head, virgin 4-day female		(46)
head, virgin 20-day female		(33)
head, mated 1-day female		(47)
head, mated 4-day female		(39)
head, mated 20-day female		28
head, mated 1-day male		(59)
head, mated 4-day male		(50)
head, mated 20-day male		(48)
salivary gland, larvae L3 wandering		(197)
salivary gland, white prepupae		(366)
digestive system, larvae L3 wandering		(298)
digestive system, 1-day adult		(246)
digestive system, 4-day adult		(205)
digestive system, 20-day adult		(187)
fat body, larvae L3 wandering		22
fat body, white prepupae		(77)
fat body, pupae P8		(85)
carcass, larvae L3 wandering		(132)
carcass, 1-day adult		32
carcass, 4-day adult		(36)
carcass, 20-day adult		(41)
ovary, virgin 4-day female		(75)
ovary, mated 4-day female		(69)
testis, mated 4-day male		(63)
accessory gland, mated 4-day male		(66)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		119
central nervous system, larvae L3		95
central nervous system, pupae P8		64
head, virgin 1-day female		46
head, virgin 4-day female		46
head, virgin 20-day female		33
head, mated 1-day female		47
head, mated 4-day female		39
head, mated 20-day female		28
head, mated 1-day male		59
head, mated 4-day male		50
head, mated 20-day male		48
salivary gland, larvae L3 wandering		(197)
salivary gland, white prepupae		(366)
digestive system, larvae L3 wandering		(298)
digestive system, 1-day adult		(246)
digestive system, 4-day adult		(205)
digestive system, 20-day adult		(187)
fat body, larvae L3 wandering		22
fat body, white prepupae		77
fat body, pupae P8		85
carcass, larvae L3 wandering		132
carcass, 1-day adult		32
carcass, 4-day adult		36
carcass, 20-day adult		41
ovary, virgin 4-day female		75
ovary, mated 4-day female		69
testis, mated 4-day male		63
accessory gland, mated 4-day male		66
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		119
central nervous system, larvae L3		95
central nervous system, pupae P8		64
head, virgin 1-day female		46
head, virgin 4-day female		46
head, virgin 20-day female		33
head, mated 1-day female		47
head, mated 4-day female		39
head, mated 20-day female		28
head, mated 1-day male		59
head, mated 4-day male		50
head, mated 20-day male		48
salivary gland, larvae L3 wandering		197
salivary gland, white prepupae		366
digestive system, larvae L3 wandering		298
digestive system, 1-day adult		246
digestive system, 4-day adult		205
digestive system, 20-day adult		187
fat body, larvae L3 wandering		22
fat body, white prepupae		77
fat body, pupae P8		85
carcass, larvae L3 wandering		132
carcass, 1-day adult		32
carcass, 4-day adult		36
carcass, 20-day adult		41
ovary, virgin 4-day female		75
ovary, mated 4-day female		69
testis, mated 4-day male		63
accessory gland, mated 4-day male		66
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed within 00-18 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		119
embryo 04-06hr		130
embryo 06-08hr		130
embryo 08-10hr		133
embryo 10-12hr		165
embryo 12-14hr		123
embryo 14-16hr		106
embryo 16-18hr		103
embryo 18-20hr		93
embryo 20-22hr		103
embryo 22-24hr		111
larva L1		78
larva L2		132
larva L3 12hr old		82
larva L3 puffstage 1-2		141
larva L3 puffstage 3-6		184
larva L3 puffstage 7-9		285
white prepupae new		266
white prepupae 12hr		161
white prepupae 24hr		124
pupae 2d postWPP		119
pupae 3d postWPP		119
pupae 4d postWPP		127
adult male 01day		76
adult male 05day		79
adult male 30day		80
adult female 01day		97
adult female 05day		96
adult female 30day		101
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(71)
embryo 02-04hr		(119)
embryo 04-06hr		(130)
embryo 06-08hr		(130)
embryo 08-10hr		(133)
embryo 10-12hr		(165)
embryo 12-14hr		(123)
embryo 14-16hr		(106)
embryo 16-18hr		(103)
embryo 18-20hr		(93)
embryo 20-22hr		(103)
embryo 22-24hr		(111)
larva L1		(78)
larva L2		(132)
larva L3 12hr old		(82)
larva L3 puffstage 1-2		(141)
larva L3 puffstage 3-6		(184)
larva L3 puffstage 7-9		(285)
white prepupae new		(266)
white prepupae 12hr		(161)
white prepupae 24hr		(124)
pupae 2d postWPP		(119)
pupae 3d postWPP		(119)
pupae 4d postWPP		(127)
adult male 01day		(76)
adult male 05day		(79)
adult male 30day		(80)
adult female 01day		(97)
adult female 05day		(96)
adult female 30day		(101)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		(119)
embryo 04-06hr		(130)
embryo 06-08hr		(130)
embryo 08-10hr		(133)
embryo 10-12hr		(165)
embryo 12-14hr		(123)
embryo 14-16hr		106
embryo 16-18hr		103
embryo 18-20hr		93
embryo 20-22hr		103
embryo 22-24hr		(111)
larva L1		78
larva L2		(132)
larva L3 12hr old		82
larva L3 puffstage 1-2		(141)
larva L3 puffstage 3-6		(184)
larva L3 puffstage 7-9		(285)
white prepupae new		(266)
white prepupae 12hr		(161)
white prepupae 24hr		(124)
pupae 2d postWPP		(119)
pupae 3d postWPP		(119)
pupae 4d postWPP		(127)
adult male 01day		76
adult male 05day		79
adult male 30day		80
adult female 01day		97
adult female 05day		96
adult female 30day		101
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		119
embryo 04-06hr		130
embryo 06-08hr		130
embryo 08-10hr		133
embryo 10-12hr		165
embryo 12-14hr		123
embryo 14-16hr		106
embryo 16-18hr		103
embryo 18-20hr		93
embryo 20-22hr		103
embryo 22-24hr		111
larva L1		78
larva L2		132
larva L3 12hr old		82
larva L3 puffstage 1-2		141
larva L3 puffstage 3-6		184
larva L3 puffstage 7-9		285
white prepupae new		266
white prepupae 12hr		161
white prepupae 24hr		124
pupae 2d postWPP		119
pupae 3d postWPP		119
pupae 4d postWPP		127
adult male 01day		76
adult male 05day		79
adult male 30day		80
adult female 01day		97
adult female 05day		96
adult female 30day		101
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		119
embryo 04-06hr		130
embryo 06-08hr		130
embryo 08-10hr		133
embryo 10-12hr		165
embryo 12-14hr		123
embryo 14-16hr		106
embryo 16-18hr		103
embryo 18-20hr		93
embryo 20-22hr		103
embryo 22-24hr		111
larva L1		78
larva L2		132
larva L3 12hr old		82
larva L3 puffstage 1-2		141
larva L3 puffstage 3-6		184
larva L3 puffstage 7-9		285
white prepupae new		266
white prepupae 12hr		161
white prepupae 24hr		124
pupae 2d postWPP		119
pupae 3d postWPP		119
pupae 4d postWPP		127
adult male 01day		76
adult male 05day		79
adult male 30day		80
adult female 01day		97
adult female 05day		96
adult female 30day		101
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(71)
embryo 02-04hr		(119)
embryo 04-06hr		(130)
embryo 06-08hr		(130)
embryo 08-10hr		(133)
embryo 10-12hr		(165)
embryo 12-14hr		(123)
embryo 14-16hr		(106)
embryo 16-18hr		(103)
embryo 18-20hr		(93)
embryo 20-22hr		(103)
embryo 22-24hr		(111)
larva L1		(78)
larva L2		(132)
larva L3 12hr old		(82)
larva L3 puffstage 1-2		(141)
larva L3 puffstage 3-6		(184)
larva L3 puffstage 7-9		(285)
white prepupae new		(266)
white prepupae 12hr		(161)
white prepupae 24hr		(124)
pupae 2d postWPP		(119)
pupae 3d postWPP		(119)
pupae 4d postWPP		(127)
adult male 01day		(76)
adult male 05day		(79)
adult male 30day		(80)
adult female 01day		(97)
adult female 05day		(96)
adult female 30day		(101)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		119
embryo 04-06hr		130
embryo 06-08hr		130
embryo 08-10hr		133
embryo 10-12hr		(165)
embryo 12-14hr		123
embryo 14-16hr		106
embryo 16-18hr		103
embryo 18-20hr		93
embryo 20-22hr		103
embryo 22-24hr		111
larva L1		78
larva L2		132
larva L3 12hr old		82
larva L3 puffstage 1-2		141
larva L3 puffstage 3-6		(184)
larva L3 puffstage 7-9		(285)
white prepupae new		(266)
white prepupae 12hr		(161)
white prepupae 24hr		124
pupae 2d postWPP		119
pupae 3d postWPP		119
pupae 4d postWPP		127
adult male 01day		76
adult male 05day		79
adult male 30day		80
adult female 01day		97
adult female 05day		96
adult female 30day		101
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		119
embryo 04-06hr		130
embryo 06-08hr		130
embryo 08-10hr		133
embryo 10-12hr		165
embryo 12-14hr		123
embryo 14-16hr		106
embryo 16-18hr		103
embryo 18-20hr		93
embryo 20-22hr		103
embryo 22-24hr		111
larva L1		78
larva L2		132
larva L3 12hr old		82
larva L3 puffstage 1-2		141
larva L3 puffstage 3-6		184
larva L3 puffstage 7-9		285
white prepupae new		266
white prepupae 12hr		161
white prepupae 24hr		124
pupae 2d postWPP		119
pupae 3d postWPP		119
pupae 4d postWPP		127
adult male 01day		76
adult male 05day		79
adult male 30day		80
adult female 01day		97
adult female 05day		96
adult female 30day		101
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		317
Schneider line 2 Sg4		360
embryonic 1182-4H		136
embryonic GM2		419
embryonic Kc167		405
embryonic S1		141
embryonic S3		143
leg disc CME L1		257
wing disc CME-W2		332
wing disc ML-DmD8		226
wing disc ML-DmD9		251
wing disc ML-DmD16-c3		263
wing disc ML-DmD21		290
wing disc ML-DmD32		231
haltere disc ML-DmD17-c3		258
eye-antennal disc ML-DmD11		236
antennal disc ML-DmD20-c5		244
mixed discs ML-DmD4-c1		206
CNS ML-DmBG1-c1		300
CNS ML-DmBG2-c2		250
tumorous blood cells mbn2		261
ovary fGS/OSS		163
ovary OSC		253
ovary OSS		129
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(317)
Schneider line 2 Sg4		(360)
embryonic 1182-4H		(136)
embryonic GM2		(419)
embryonic Kc167		(405)
embryonic S1		(141)
embryonic S3		(143)
leg disc CME L1		(257)
wing disc CME-W2		(332)
wing disc ML-DmD8		(226)
wing disc ML-DmD9		(251)
wing disc ML-DmD16-c3		(263)
wing disc ML-DmD21		(290)
wing disc ML-DmD32		(231)
haltere disc ML-DmD17-c3		(258)
eye-antennal disc ML-DmD11		(236)
antennal disc ML-DmD20-c5		(244)
mixed discs ML-DmD4-c1		(206)
CNS ML-DmBG1-c1		(300)
CNS ML-DmBG2-c2		(250)
tumorous blood cells mbn2		(261)
ovary fGS/OSS		(163)
ovary OSC		(253)
ovary OSS		(129)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(317)
Schneider line 2 Sg4		(360)
embryonic 1182-4H		(136)
embryonic GM2		(419)
embryonic Kc167		(405)
embryonic S1		(141)
embryonic S3		(143)
leg disc CME L1		(257)
wing disc CME-W2		(332)
wing disc ML-DmD8		(226)
wing disc ML-DmD9		(251)
wing disc ML-DmD16-c3		(263)
wing disc ML-DmD21		(290)
wing disc ML-DmD32		(231)
haltere disc ML-DmD17-c3		(258)
eye-antennal disc ML-DmD11		(236)
antennal disc ML-DmD20-c5		(244)
mixed discs ML-DmD4-c1		(206)
CNS ML-DmBG1-c1		(300)
CNS ML-DmBG2-c2		(250)
tumorous blood cells mbn2		(261)
ovary fGS/OSS		(163)
ovary OSC		(253)
ovary OSS		(129)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		317
Schneider line 2 Sg4		360
embryonic 1182-4H		136
embryonic GM2		419
embryonic Kc167		405
embryonic S1		141
embryonic S3		143
leg disc CME L1		257
wing disc CME-W2		332
wing disc ML-DmD8		226
wing disc ML-DmD9		251
wing disc ML-DmD16-c3		263
wing disc ML-DmD21		290
wing disc ML-DmD32		231
haltere disc ML-DmD17-c3		258
eye-antennal disc ML-DmD11		236
antennal disc ML-DmD20-c5		244
mixed discs ML-DmD4-c1		206
CNS ML-DmBG1-c1		300
CNS ML-DmBG2-c2		250
tumorous blood cells mbn2		261
ovary fGS/OSS		163
ovary OSC		253
ovary OSS		129
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		317
Schneider line 2 Sg4		360
embryonic 1182-4H		136
embryonic GM2		419
embryonic Kc167		405
embryonic S1		141
embryonic S3		143
leg disc CME L1		257
wing disc CME-W2		332
wing disc ML-DmD8		226
wing disc ML-DmD9		251
wing disc ML-DmD16-c3		263
wing disc ML-DmD21		290
wing disc ML-DmD32		231
haltere disc ML-DmD17-c3		258
eye-antennal disc ML-DmD11		236
antennal disc ML-DmD20-c5		244
mixed discs ML-DmD4-c1		206
CNS ML-DmBG1-c1		300
CNS ML-DmBG2-c2		250
tumorous blood cells mbn2		261
ovary fGS/OSS		163
ovary OSC		253
ovary OSS		129
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(317)
Schneider line 2 Sg4		(360)
embryonic 1182-4H		(136)
embryonic GM2		(419)
embryonic Kc167		(405)
embryonic S1		(141)
embryonic S3		(143)
leg disc CME L1		(257)
wing disc CME-W2		(332)
wing disc ML-DmD8		(226)
wing disc ML-DmD9		(251)
wing disc ML-DmD16-c3		(263)
wing disc ML-DmD21		(290)
wing disc ML-DmD32		(231)
haltere disc ML-DmD17-c3		(258)
eye-antennal disc ML-DmD11		(236)
antennal disc ML-DmD20-c5		(244)
mixed discs ML-DmD4-c1		(206)
CNS ML-DmBG1-c1		(300)
CNS ML-DmBG2-c2		(250)
tumorous blood cells mbn2		(261)
ovary fGS/OSS		(163)
ovary OSC		(253)
ovary OSS		(129)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(317)
Schneider line 2 Sg4		(360)
embryonic 1182-4H		136
embryonic GM2		(419)
embryonic Kc167		(405)
embryonic S1		141
embryonic S3		143
leg disc CME L1		(257)
wing disc CME-W2		(332)
wing disc ML-DmD8		(226)
wing disc ML-DmD9		(251)
wing disc ML-DmD16-c3		(263)
wing disc ML-DmD21		(290)
wing disc ML-DmD32		(231)
haltere disc ML-DmD17-c3		(258)
eye-antennal disc ML-DmD11		(236)
antennal disc ML-DmD20-c5		(244)
mixed discs ML-DmD4-c1		(206)
CNS ML-DmBG1-c1		(300)
CNS ML-DmBG2-c2		(250)
tumorous blood cells mbn2		(261)
ovary fGS/OSS		(163)
ovary OSC		(253)
ovary OSS		129
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		317
Schneider line 2 Sg4		360
embryonic 1182-4H		136
embryonic GM2		419
embryonic Kc167		405
embryonic S1		141
embryonic S3		143
leg disc CME L1		257
wing disc CME-W2		332
wing disc ML-DmD8		226
wing disc ML-DmD9		251
wing disc ML-DmD16-c3		263
wing disc ML-DmD21		290
wing disc ML-DmD32		231
haltere disc ML-DmD17-c3		258
eye-antennal disc ML-DmD11		236
antennal disc ML-DmD20-c5		244
mixed discs ML-DmD4-c1		206
CNS ML-DmBG1-c1		300
CNS ML-DmBG2-c2		250
tumorous blood cells mbn2		261
ovary fGS/OSS		163
ovary OSC		253
ovary OSS		129
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		52
cold shock, 4-day adult		54
heat shock, 4-day adult		108
Cadmium 50 mM 6 hrs, larvae L3		148
Cadmium 50 mM 12 hrs, larvae L3		227
Cadmium 50 mM 48 hrs, 4-day adult		104
Cadmium 100 mM 48 hrs, 4-day adult		95
Copper 0.5 mM 12 hrs, larvae L3		79
Copper 15 mM 48 hrs, 4-day adult		139
Zinc 5 mM 12 hrs, larvae L3		62
Zinc 4.5 mM 48 hrs, 4-day adult		114
Ethanol 2.5% 3 hrs, larvae L3		65
Ethanol 5% 3 hrs, larvae L3		196
Ethanol 10% 3 hrs, larvae L3		125
Caffeine 1.5 mg/ml 4 hrs, larvae L3		124
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		80
Caffeine 25 mg/ml 48 hrs, 4-day adult		49
Paraquat 5 mM 48 hrs, 4-day adult		81
Paraquat 10 mM 48 hrs, 4-day adult		96
Rotenone 2 μg 12 hrs, larvae L3		186
Rotenone 8 μg 12 hrs, larvae L3		140
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(52)
cold shock, 4-day adult		(54)
heat shock, 4-day adult		(108)
Cadmium 50 mM 6 hrs, larvae L3		(148)
Cadmium 50 mM 12 hrs, larvae L3		(227)
Cadmium 50 mM 48 hrs, 4-day adult		(104)
Cadmium 100 mM 48 hrs, 4-day adult		(95)
Copper 0.5 mM 12 hrs, larvae L3		(79)
Copper 15 mM 48 hrs, 4-day adult		(139)
Zinc 5 mM 12 hrs, larvae L3		(62)
Zinc 4.5 mM 48 hrs, 4-day adult		(114)
Ethanol 2.5% 3 hrs, larvae L3		(65)
Ethanol 5% 3 hrs, larvae L3		(196)
Ethanol 10% 3 hrs, larvae L3		(125)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(124)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(80)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(49)
Paraquat 5 mM 48 hrs, 4-day adult		(81)
Paraquat 10 mM 48 hrs, 4-day adult		(96)
Rotenone 2 μg 12 hrs, larvae L3		(186)
Rotenone 8 μg 12 hrs, larvae L3		(140)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		52
cold shock, 4-day adult		54
heat shock, 4-day adult		(108)
Cadmium 50 mM 6 hrs, larvae L3		(148)
Cadmium 50 mM 12 hrs, larvae L3		(227)
Cadmium 50 mM 48 hrs, 4-day adult		104
Cadmium 100 mM 48 hrs, 4-day adult		95
Copper 0.5 mM 12 hrs, larvae L3		79
Copper 15 mM 48 hrs, 4-day adult		(139)
Zinc 5 mM 12 hrs, larvae L3		62
Zinc 4.5 mM 48 hrs, 4-day adult		(114)
Ethanol 2.5% 3 hrs, larvae L3		65
Ethanol 5% 3 hrs, larvae L3		(196)
Ethanol 10% 3 hrs, larvae L3		(125)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(124)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		80
Caffeine 25 mg/ml 48 hrs, 4-day adult		49
Paraquat 5 mM 48 hrs, 4-day adult		81
Paraquat 10 mM 48 hrs, 4-day adult		96
Rotenone 2 μg 12 hrs, larvae L3		(186)
Rotenone 8 μg 12 hrs, larvae L3		(140)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		52
cold shock, 4-day adult		54
heat shock, 4-day adult		108
Cadmium 50 mM 6 hrs, larvae L3		148
Cadmium 50 mM 12 hrs, larvae L3		227
Cadmium 50 mM 48 hrs, 4-day adult		104
Cadmium 100 mM 48 hrs, 4-day adult		95
Copper 0.5 mM 12 hrs, larvae L3		79
Copper 15 mM 48 hrs, 4-day adult		139
Zinc 5 mM 12 hrs, larvae L3		62
Zinc 4.5 mM 48 hrs, 4-day adult		114
Ethanol 2.5% 3 hrs, larvae L3		65
Ethanol 5% 3 hrs, larvae L3		196
Ethanol 10% 3 hrs, larvae L3		125
Caffeine 1.5 mg/ml 4 hrs, larvae L3		124
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		80
Caffeine 25 mg/ml 48 hrs, 4-day adult		49
Paraquat 5 mM 48 hrs, 4-day adult		81
Paraquat 10 mM 48 hrs, 4-day adult		96
Rotenone 2 μg 12 hrs, larvae L3		186
Rotenone 8 μg 12 hrs, larvae L3		140
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		52
cold shock, 4-day adult		54
heat shock, 4-day adult		108
Cadmium 50 mM 6 hrs, larvae L3		148
Cadmium 50 mM 12 hrs, larvae L3		227
Cadmium 50 mM 48 hrs, 4-day adult		104
Cadmium 100 mM 48 hrs, 4-day adult		95
Copper 0.5 mM 12 hrs, larvae L3		79
Copper 15 mM 48 hrs, 4-day adult		139
Zinc 5 mM 12 hrs, larvae L3		62
Zinc 4.5 mM 48 hrs, 4-day adult		114
Ethanol 2.5% 3 hrs, larvae L3		65
Ethanol 5% 3 hrs, larvae L3		196
Ethanol 10% 3 hrs, larvae L3		125
Caffeine 1.5 mg/ml 4 hrs, larvae L3		124
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		80
Caffeine 25 mg/ml 48 hrs, 4-day adult		49
Paraquat 5 mM 48 hrs, 4-day adult		81
Paraquat 10 mM 48 hrs, 4-day adult		96
Rotenone 2 μg 12 hrs, larvae L3		186
Rotenone 8 μg 12 hrs, larvae L3		140
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(52)
cold shock, 4-day adult		(54)
heat shock, 4-day adult		(108)
Cadmium 50 mM 6 hrs, larvae L3		(148)
Cadmium 50 mM 12 hrs, larvae L3		(227)
Cadmium 50 mM 48 hrs, 4-day adult		(104)
Cadmium 100 mM 48 hrs, 4-day adult		(95)
Copper 0.5 mM 12 hrs, larvae L3		(79)
Copper 15 mM 48 hrs, 4-day adult		(139)
Zinc 5 mM 12 hrs, larvae L3		(62)
Zinc 4.5 mM 48 hrs, 4-day adult		(114)
Ethanol 2.5% 3 hrs, larvae L3		(65)
Ethanol 5% 3 hrs, larvae L3		(196)
Ethanol 10% 3 hrs, larvae L3		(125)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(124)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(80)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(49)
Paraquat 5 mM 48 hrs, 4-day adult		(81)
Paraquat 10 mM 48 hrs, 4-day adult		(96)
Rotenone 2 μg 12 hrs, larvae L3		(186)
Rotenone 8 μg 12 hrs, larvae L3		(140)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		52
cold shock, 4-day adult		54
heat shock, 4-day adult		108
Cadmium 50 mM 6 hrs, larvae L3		(148)
Cadmium 50 mM 12 hrs, larvae L3		(227)
Cadmium 50 mM 48 hrs, 4-day adult		104
Cadmium 100 mM 48 hrs, 4-day adult		95
Copper 0.5 mM 12 hrs, larvae L3		79
Copper 15 mM 48 hrs, 4-day adult		139
Zinc 5 mM 12 hrs, larvae L3		62
Zinc 4.5 mM 48 hrs, 4-day adult		114
Ethanol 2.5% 3 hrs, larvae L3		65
Ethanol 5% 3 hrs, larvae L3		(196)
Ethanol 10% 3 hrs, larvae L3		125
Caffeine 1.5 mg/ml 4 hrs, larvae L3		124
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		80
Caffeine 25 mg/ml 48 hrs, 4-day adult		49
Paraquat 5 mM 48 hrs, 4-day adult		81
Paraquat 10 mM 48 hrs, 4-day adult		96
Rotenone 2 μg 12 hrs, larvae L3		(186)
Rotenone 8 μg 12 hrs, larvae L3		140
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		52
cold shock, 4-day adult		54
heat shock, 4-day adult		108
Cadmium 50 mM 6 hrs, larvae L3		148
Cadmium 50 mM 12 hrs, larvae L3		227
Cadmium 50 mM 48 hrs, 4-day adult		104
Cadmium 100 mM 48 hrs, 4-day adult		95
Copper 0.5 mM 12 hrs, larvae L3		79
Copper 15 mM 48 hrs, 4-day adult		139
Zinc 5 mM 12 hrs, larvae L3		62
Zinc 4.5 mM 48 hrs, 4-day adult		114
Ethanol 2.5% 3 hrs, larvae L3		65
Ethanol 5% 3 hrs, larvae L3		196
Ethanol 10% 3 hrs, larvae L3		125
Caffeine 1.5 mg/ml 4 hrs, larvae L3		124
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		80
Caffeine 25 mg/ml 48 hrs, 4-day adult		49
Paraquat 5 mM 48 hrs, 4-day adult		81
Paraquat 10 mM 48 hrs, 4-day adult		96
Rotenone 2 μg 12 hrs, larvae L3		186
Rotenone 8 μg 12 hrs, larvae L3		140
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004687

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG3201-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

Mlc-c^GD14991

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Mlc-c	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Mlc-c	Mutagen	Stocks	Known lesion
Mlc-c^GD14991	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Mlc-c^KK107927	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Mlc-c^{Ubi.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14991}

P{KK107927}

characterization construct

P{Ubi-Mlc-c.myc}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )

Terms Based on Experimental Evidence ( 8 terms )

Molecular Function

CV term

References

myosin binding

inferred from physical interaction with didum

myosin heavy chain binding

inferred from physical interaction with ck

inferred from physical interaction with didum

(Toth et al., 2005)

inferred from physical interaction with jar

inferred from physical interaction with zip

protein binding

inferred from physical interaction with asp

Biological Process

CV term

actin filament-based movement

References

inferred from direct assay

(Toth et al., 2005)

Cellular Component

CV term

References

myosin II complex

inferred from physical interaction with zip

myosin V complex

inferred from physical interaction with didum

myosin VI complex

inferred from physical interaction with jar

myosin VII complex

inferred from physical interaction with ck

Terms Based on Predictions or Assertions ( 4 terms )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

calcium ion binding

inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR011992

Biological Process

CV term

actin filament-based movement

References

inferred by curator from GO:0016460

Cellular Component

CV term

(Goldstein and Gunawardena, 2000)

References

myosin complex

inferred from sequence or structural similarity

inferred from sequence or structural similarity with UniProtKB:P14649

(FlyBase, 1992-)

unconventional myosin complex

non-traceable author statement

(Swiss-Prot Project Members, 1996.10.1)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

Mlc-c - CG10194

experimental knowledge based

Mlc-c - CG11459

experimental knowledge based

Mlc-c - CG12691

experimental knowledge based

Mlc-c - Fibp

experimental knowledge based

Mlc-c - Pglym87

experimental knowledge based

Mlc-c - Tm1

experimental knowledge based

Mlc-c - zip

experimental knowledge based

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Mlc-c allele

Gene

References

External Data

BioGRID - A database of protein and genetic interactions

•

57980

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0004687

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31474

Orthologs

OrthoDB Orthologs (77) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG618C7Z

Dipteran inclusive ortholog search

EOG6X6CJP

Insect inclusive ortholog search

EOG6F1VZX

Arthropod inclusive ortholog search

EOG69W0X3

Metazoa inclusive ortholog search

EOG65TRH5

Orthologs in Drosophila Species (EOG618C7Z)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA22593

Drosophila persimilis

Dper\GL16470

Drosophila willistoni

Dwil\GK24961

Drosophila virilis

Dvir\GJ16948

Drosophila mojavensis

Dmoj\GI21775

Drosophila grimshawi

Dgri\GH24922

Orthologs in non-Drosophila Dipterans (EOG6X6CJP)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007439

Anopheles gambiae

Malaria mosquito

Agam\AGAP007806

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ801935

Orthologs in non-Dipteran Insects (EOG6F1VZX)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB54761

Apis mellifera

Western honey bee

Amel\GB54760

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG034756

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG021039

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH17321

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH26968

Atta cephalotes

Leafcutter ant

Acep\ACEP13002

Atta cephalotes

Leafcutter ant

Acep\ACEP13001

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO19752

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO19751

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL16787

Linepithema humile

Argentine ant

Lhum\LH18335

Linepithema humile

Argentine ant

Lhum\LH18338

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB15657

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB15660

Solenopsis invicta

Red fire ant

Sinv\SINV16107

Solenopsis invicta

Red fire ant

Sinv\SINV16137

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004388

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008358

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005933

Bombyx mori

Silkmoth

Bmor\BGIBMGA005853

Pediculus humanus

Human body louse

Phum\PHUM172560

Pediculus humanus

Human body louse

Phum\PHUM065500

Tribolium castaneum

Red flour beetle

Tcas\TC008661

Tribolium castaneum

Red flour beetle

Tcas\TC009606

Orthologs in non-Insect Arthropods (EOG69W0X3)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\Arhgef15

Daphnia pulex

Water flea

Dpux\JGI_V11_224783

Ixodes scapularis

Deer tick

Isca\ISCW001124

Ixodes scapularis

Deer tick

Isca\ISCW023121

Orthologs in non-Arthropod Metazoa (EOG65TRH5)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\mlc-3

Caenorhabditis elegans

Nematode

Cele\K04C1.4

Caenorhabditis elegans

Nematode

Cele\mlc-5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001613gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_007247gn

Danio rerio

Zebrafish

Drer\myl6

Danio rerio

Zebrafish

Drer\zgc:66286

Danio rerio

Zebrafish

Drer\myl1

Danio rerio

Zebrafish

Drer\cmlc1

Danio rerio

Zebrafish

Drer\zgc:163073

Danio rerio

Zebrafish

Drer\zgc:114164

Danio rerio

Zebrafish

Drer\mylz3

Xenopus tropicalis

Western clawed frog

Xtro\myl1

Xenopus tropicalis

Western clawed frog

Xtro\myl6

Xenopus tropicalis

Western clawed frog

Xtro\myl3

Xenopus tropicalis

Western clawed frog

Xtro\myl4

Gallus gallus

Domestic chicken

Ggal\MYL4

Gallus gallus

Domestic chicken

Ggal\MYL1

Gallus gallus

Domestic chicken

Ggal\MYL3

Mus musculus

House mouse

Mmus\Myl3

Mus musculus

House mouse

Mmus\Myl4

Mus musculus

House mouse

Mmus\Gm8894

Mus musculus

House mouse

Mmus\Myl6

Mus musculus

House mouse

Mmus\Myl6b

Rattus norvegicus

Norway rat

Rnor\Myl3

Rattus norvegicus

Norway rat

Rnor\Myl1

Rattus norvegicus

Norway rat

Rnor\Myl6

Rattus norvegicus

Norway rat

Rnor\LOC684533

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000042931

Homo sapiens

Human

Hsap\MYL6B

Homo sapiens

Human

Hsap\MYL6

Homo sapiens

Human

Hsap\MYL3

Homo sapiens

Human

Hsap\ENSG00000168530

Human Orthologs (4)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA22593

Drosophila persimilis

Dper\GL16470

Drosophila willistoni

Dwil\GK24961

Drosophila virilis

Dvir\GJ16948

Drosophila mojavensis

Dmoj\GI21775

Drosophila grimshawi

Dgri\GH24922

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v107177

P{KK107927}VIE-260B

v30035

w¹¹¹⁸; P{GD14991}v30035/TM3

v31160

w¹¹¹⁸; P{GD14991}v31160/TM3

Genomic Clones ( 1 )

BACR27J18

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 112 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

(Goldstein and Gunawardena, 2000, Keleman et al., 2009.8.5, Cammarato et al., 2011)

DRSC - Results from RNAi screens.

•

FBgn0004687

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31474

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Mlc-c CG3201

(Zhong, 1999.11)

Source for database merge of

Additional comments

An intron of Mlc-c contains a hairpin bearing a 3' tail which has the characteristics of a tailed mirtron, producing miRNA/miRNA* duplexes. This mirtron sequence is represented by mir-3645.

(Flynt et al., 2010)

Other Comments

The structural gene for the alkali light chain of non-muscle myosin (whose other subunits are encoded by zip and sqh).

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

31474

UniProtKB/Swiss-Prot

•

P54357

UniProtKB/TrEMBL

•

Q95SW7

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

EF-Hand 1, calcium-binding site (IPR018247)

EF-hand domain (IPR002048)

EF-hand-like domain (IPR011992)

Linkouts

BioGRID - A database of protein and genetic interactions

•

57980

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0004687

DRSC - Results from RNAi screens.

•

FBgn0004687

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004687

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG3201-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0004687

FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways

•

95312

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31474

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31474

modMine - Data generated by the modENCODE project.

•

FBgn0004687

Synonyms & Secondary IDs ( 16 )

Reported As

Symbol Synonym

CG3201

DmMLC_C

(Yamashita et al., 2000)

Mlc-c

(Toth et al., 2005, Todi et al., 2008, Satoh et al., 2008, Asensio et al., 2010, Wasbrough et al., 2010, Kaushik et al., 2012)

mlc-c

(Franke et al., 2006, Polevoy et al., 2009, Franke et al., 2010, Flynt et al., 2010)

MyoII

(Pichaud et al., 2007)

nmELC

(Yamashita et al., 2000)

Name Synonym

alkali light chain of non-muscle myosin-II

(Edwards et al., 1991)

essential light chain-cytoplasmic

essential myosin light chain-cytoplasmic