A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Gene Dmel\Mlc-c

General Information
SymbolDmel\Mlc-cSpeciesD. melanogaster
NameMyosin light chain cytoplasmicAnnotation symbolCG3201
Feature typeprotein_coding_geneFlyBase IDFBgn0004687
Gene Model StatusCurrent Stock availability 3 publicly available
Genomic Location
Chromosome (arm)XRecombination map
Cytogenetic map5A8-5A8Sequence locationX:5,513,169..5,516,591 [-]

Genomic Maps

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modENCODE GBrowse
detailed view
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Automatically generated summary

See sections below for more information
The gene Myosin light chain cytoplasmic is referred to in FlyBase by the symbol Dmel\Mlc-c (CG3201, FBgn0004687). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: myosin heavy chain binding; myosin binding. The biological processes in which it is involved are not known. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Calcium-binding EF-hand; EF-HAND 2; EF-Hand 1, calcium-binding site; EF-hand-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed within 00-18 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631832_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Mlc-c. Gene sequence location is X:5513169..5516591.
hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
Mlc-c: Myosin alkali light chain cytoplasmic (D.P. Kiehart)
The structural gene for the alkali light chain of non-muscle myosin (whose other subunits are encoded by Mhc-c and sqh).
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
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FB2012_01
References
Franke et al., 2010, Dev. Biol. 345(2): 117--132
Sequence features
BKN46413
HFA18683
BKN27927
FB2011_10
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
5A8-5A8  
Limits computationally determined from genome sequence between P{EP}CG3249EP1400 and P{EP}Tre1EP496  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
hide Gene Model & Products
Please see the GBrowse view of Dmel\Mlc-c for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
detailed view FBtr0070784 FBtr0070785 FBtr0308579 FBtr0309642 FBtr0309642 FBtr0309642 FBtr0070783 FBtr0070782 FBpp0070751 FBpp0070750 FBpp0300803 FBpp0070749 FBpp0070748 FBti0042568 FBti0012836 FBti0013133 FBti0114765
Comments on Gene Model
Gene model reviewed during 5.39
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Mlc-c-RA
FBtr0070783
 NM_078494
  1321
  147
Mlc-c-RB
FBtr0308579
 
  1200
  153
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Mlc-c-PA  
FBpp0070749  
16.6  
147  
4.22  
  NP_511049
  AAF46048
Mlc-c-PB  
FBpp0300803  
17.5  
153  
4.41  
 
 
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
EF-Hand 1, calcium-binding site (IPR018247)
EF-HAND 2 (IPR018249)
Calcium-binding EF-hand (IPR002048)
EF-hand-like domain (IPR011992)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA201383
 
AA392475
 
AA438556
 
AA540721
 
AA695602
 
AA735245
 
AA816603
 
AA816664
 
AE014298
AAF46048
 
AI531273
 
AI533038
 
AW944225
 
AY060455
AAL25494
 
AY433527
 
AY441283
 
BE976539
 
BG637988
 
BG639015
 
BI165257
 
BI171471
 
BI212912
 
BI213725
 
BI214156
 
BI215862
 
BI216773
 
BI230673
 
BI235211
 
BI240297
 
BI242038
 
BI356343
 
BI361469
 
BI362275
 
BI363830
 
BI365393
 
BI366348
 
BI367909
 
BI369780
 
BI373309
 
BI373310
 
BI373850
 
BI374474
 
BI482547
 
BI483408
 
BI626506
 
BI632328
 
BI632899
 
BI637773
 
BP541051
 
BP544167
 
BP544168
 
BT082023
 
BX014246
 
BX045002
 
BX055577
 
CA803870
 
CA804358
 
CA804369
 
CO181768
 
CO186919
 
CO186998
 
CO192527
 
CO193389
 
CO194654
 
CO262224
 
CO262638
 
CO283440
 
CO287707
 
CO290552
 
CO291137
 
CO295619
 
CO303667
 
CO303840
 
CO308580
 
CO309978
 
CO310229
 
CO320909
 
CO324305
 
CO329537
 
CO333312
 
CO333703
 
CO336492
 
CO336894
 
CO338159
 
CO340920
 
DN154186
 
DN154360
 
DQ440082
 
EC057444
 
EC059109
 
EC059133
 
EC060898
 
EC063307
 
EC066528
 
EC076334
 
EC079352
 
EC084450
 
EC090141
 
EC195170
 
EC196581
 
EC198436
 
EC201605
 
EC203066
 
EC211307
 
EC212198
 
EC212859
 
EC213406
 
EC213445
 
EC214384
 
EC216189
 
EC216532
 
EC218896
 
EC219455
 
EC223216
 
EC229400
 
EC236886
 
EC239904
 
EC249587
 
EC253416
 
EC263951
 
EC264197
 
EC265260
 
EC266927
 
EV578394
 
EV591953
 
EV594020
 
U25057
AAA84897
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
hide Mapped Features
Mapped Features have been reorganized, please see this article for details.
Additional mapped features and mutations can be found on GBrowse or related reports.
Type
Symbol & Location
Additional Notes
References
hide External Data
Linkouts
Crossreferences
hide Expression Data
hideTranscript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
hidePolypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male  
spermatozoon  
(Wasbrough et al., 2010)
day 7 of adulthood -- day 49 of adulthood  
adult heart  
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
hide High-Throughput Expression Data
or
Untitled Document detailed view RhoGAP5A-RB RhoGAP5A-RA Mlc-c-RB mir-3645-RM Mlc-c-RA l(1)G0045-RA
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
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modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
hide Expression Clusters
A cluster of genes with similar mRNA expression dynamics across development.
(The modENCODE Consortium, 2010)
hide External Data & Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
•
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
•
hide Alleles & Phenotypes
hide Summary of Allele Phenotypes
Lethality
Allele
viable, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
hide Classical Alleles ( 0 )
For All Classical Alleles Show

Allele of Mlc-cClassMutagenStocksKnown lesion
hide Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show

Allele of Mlc-cClassMutagenStocksKnown lesion
Mlc-cGD149912 Yes
Mlc-cKK1079271 Yes
Mlc-cUbi.T:Hsap\MYC0 Yes
hide Aneuploid Aberrations
hide Transgenic Constructs & Insertions
Transgenic Constructs
Type of construct
Name
Expression data
UAS construct
P{GD14991}
NA
P{KK107927}
NA
characterization construct
P{Ubi-Mlc-c.myc}
NA
Insertions
Type of insertions
Name
Expression data
hide Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
hide Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term
Evidence
References
myosin binding
inferred from physical interaction with didum
(Franke et al., 2006)
myosin heavy chain binding
inferred from physical interaction with ck
(Franke et al., 2006)
inferred from physical interaction with didum
(Toth et al., 2005)
inferred from physical interaction with jar
(Franke et al., 2006)
inferred from physical interaction with zip
(Franke et al., 2006)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
hide Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term
Evidence
References
ATPase activity, coupled
inferred from sequence or structural similarity
(Goldstein and Gunawardena, 2000)
calcium ion binding
inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR011992
(FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component
CV term
Evidence
References
myosin complex
inferred from sequence or structural similarity
(Goldstein and Gunawardena, 2000)
inferred from sequence or structural similarity with UniProtKB:P14649
(FlyBase, 1992-)
unconventional myosin complex
non-traceable author statement
(Swiss-Prot Project Members, 1996.10.1)
hide Sequence Ontology: Class of Gene
nuclear_gene
 
protein_coding_gene
 
hide Interactions & Pathways
hide Summary of Physical Interactions
Protein-protein
Interacting group
Assay
References
hide Summary of Genetic Interactions
Interacts with
Please look at the allele data for full details of the genetic interactions
Mlc-c allele
Gene
References
hide External Data
Linkouts
BioGRID - A database of protein and genetic interactions
•
DroID - A comprehensive database of gene and protein interactions.
•
InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.
•
hide Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs
Linkouts
InParanoid A subset of ortholog calls from InParanoid.
•
OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.
•
hide Stocks & Reagents
hide Stocks Listed in FlyBase ( 3 )
VDRC
v107177
P{KK107927}VIE-260B
v30035
w1118; P{GD14991}v30035/TM3
v31160
w1118; P{GD14991}v31160/TM3
hide Genomic Clones ( 1 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
hide cDNA Clones ( 112 )

Please Note
This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
hide RNAi & Array Information
Linkouts
DRSC - Results from RNAi screens.
•
GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
•
hide Antibody Information
hide Other Information
hide Discoverer
hide Etymology
hide Identification
hide Relationship to Other Genes
Source for database identity of
Source for identity of: Mlc-c CG3201
(Zhong, 1999.11)
Source for database merge of
Additional comments
An intron of Mlc-c contains a hairpin bearing a 3' tail which has the characteristics of a tailed mirtron, producing miRNA/miRNA* duplexes. This mirtron sequence is represented by mir-3645.
(Flynt et al., 2010)
hide Other Comments
The structural gene for the alkali light chain of non-muscle myosin (whose other subunits are encoded by zip and sqh).
 
hide External Crossreferences & Linkouts
Sequence Crossreferences
RefSeq (Transcripts)
•
RefSeq (Proteins)
•
DDBJ / EMBL / GenBank
•
Entrez Gene - A searchable database of RefSeq genes.
•
UniProtKB/Swiss-Prot
•
UniProtKB/TrEMBL
•
Other Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
EF-Hand 1, calcium-binding site (IPR018247)
EF-HAND 2 (IPR018249)
Calcium-binding EF-hand (IPR002048)
EF-hand-like domain (IPR011992)
Linkouts
BioGRID - A database of protein and genetic interactions
•
DroID - A comprehensive database of gene and protein interactions.
•
DRSC - Results from RNAi screens.
•
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
•
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
•
FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
•
FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways
•
GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
•
InParanoid A subset of ortholog calls from InParanoid.
•
InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.
•
modMine - Data generated by the modENCODE project.
•
OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.
•
hide Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym
CG3201
(Goldstein and Gunawardena, 2000, Keleman et al., 2009.8.5, Cammarato et al., 2011)
DmMLC_C
(Yamashita et al., 2000)
Mlc-c
(Toth et al., 2005, Todi et al., 2008, Satoh et al., 2008, Asensio et al., 2010, Wasbrough et al., 2010)
mlc-c
(Franke et al., 2006, Polevoy et al., 2009, Franke et al., 2010, Flynt et al., 2010)
MyoII
(Pichaud et al., 2007)
nmELC
(Yamashita et al., 2000)
Name Synonym
alkali light chain of non-muscle myosin-II
(Edwards et al., 1991)
essential light chain-cytoplasmic
(Franke et al., 2006)
essential myosin light chain-cytoplasmic
(Franke et al., 2006)
Myosin
(Wang and Riechmann, 2007)
Myosin-II
(Lecuit et al., 2007)
Myosin II
(Straight et al., 2005)
myosin II
(Lecuit, 2004)
Myosin light chain cytoplasmic
(Cammarato et al., 2011)
Myosin light chain-cytoplasmic
(Satoh et al., 2008)
non-muscle Myosin II
(Pichaud et al., 2007)
Secondary FlyBase IDs
hide References ( 37 )
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hide Recent research papers ( 4 )
Morozova et al., 2011, Genetics 187(4): 1193--1205
Transcriptional networks for alcohol sensitivity in Drosophila melanogaster. [FBrf0214377]
Asensio et al., 2010, J. Cell Biol. 191(6): 1173--1187
RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway. [FBrf0212515]
Flynt et al., 2010, Mol. Cell 38(6): 900--907
MicroRNA Biogenesis via Splicing and Exosome-Mediated Trimming in Drosophila. [FBrf0211240]
Franke et al., 2010, Dev. Biol. 345(2): 117--132
Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis. [FBrf0211576]
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