PKC, eye-PKC, protein kinase C, PKCi, dPKC53E(ey)
Gene model reviewed during 5.50
There is only one protein coding transcript and one polypeptide associated with this gene
Tetrapeptide ligand at C-terminus is tethered to inaD by interaction with the second PDZ domain.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\inaC using the Feature Mapper tool.
Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.
inaC protein is localized to the photoreceptor cells in both compound eyes and ocelli. Most of the signal is confined to the rhabdomeres of the photoreceptor cells. An inaCFBtr0091350:pb-XRlacZ reporter construct was used to show that it is expressed in all types of photoreceptor cells in the eye and ocelli.
GBrowse - Visual display of RNA-Seq signalsView Dmel\inaC in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in mitotic index seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The second PDZ domain of inaD protein interacts with the last three residues in the carboxyl-terminal tail of inaC protein. The in vivo activity of inaC protein depends on its association with inaD protein.
Intracellular voltage clamping demonstrated that light adaptation is severely reduced in inaC mutants, suggesting that the eye specific PKC encoded by inaC is required for adaptation. Whole cell current recordings from isolated ommatidia showed that in the absence of PKC the single photon-induced quantum bumps fail to terminate normally.
The protein kinase C (PKC) enzyme in Drosophila is encoded by a gene family made up of three genes which are expressed primarily in adult flies. The deduced amino acid sequences of these genes are quite similar to each other and those of the mammalian PKC family (Schaeffer et al., 1989).
Structural gene for protein kinase C (PKC). Isolated from Drosophila clones obtained with mammalian probes. Expressed primarily in photoreceptors of adult flies. Mutant alleles have same basic phenotype as inaA; also, inaC lacks off transient of ERG and (in such physiological recordings) there is a slow return to baseline potential at stimulus offset. Structural gene for protein kinase C (PKC); expressed primarily in photoreceptors of adult flies. Mutant alleles have same basic phenotype as inaA; also, inaC lacks off transient of ERG and (in such physiological recordings) there is a slow return to baseline potential at stimulus offset.