Gene Dmel\His3.3B

(Akhmanova et al., 1995, Fretzin et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

His3.3B-PA

FBpp0071280

15.3

136

11.83

NP_511095

AAF46452

His3.3B-PB

FBpp0071281

15.3

136

11.83

NP_727314

AAN09245

His3.3B-PC

FBpp0071282

15.3

136

11.83

NP_788892

AAO41645

His3.3B-PE

FBpp0309302

15.3

136

11.83

Additional Polypeptide Data & Comments

Reported size (kDa)

136 (aa)

Comments

His3.3A protein and His3.3B protein are identical in amino acid sequence. The genes differ substantially in their untranslated regions.

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Histone H3 (IPR000164)

Histone core (IPR007125)

Histone-fold (IPR009072)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P84249

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

adult stage | female

adult stage | male

oogenesis & adult stage | female

ovary

spermatogenesis & adult stage

testis

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval salivary gland

• polytene chromosome

spermatogenesis & adult stage

protein body

Y chromosome loop

spermatid

spermatocyte

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Antibodies against His3.3 protein stain uniformly over the polytene chromosomes. His3.3 protein staining is seen in meiotic prophase chromatin of primary spermatocytes. One or two strongly staining foci were observed in each nucleus. The distribution of label coincides with the location of some of the Y-chromosomal lampbrush loops. At this stage His3 protein is observed mainly in the autosomal chromatin. In postmeiotic stages, His3.3 protein is observed in the protein body while the His3 protein is found mainly in the chromatin. During spermatid elongation, His3.3 protein is deposited in chromatin. At subsequent stages no major differences were observed in the distribution of His3 protein and His3.3 protein. In post-elongation spermatid cysts, the pattern of staining changes from an even distribution to a dispersed pattern. In mature sperm, no staining is observed.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(The modENCODE Consortium, 2010)

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval midgut, adult fat body, virgin spermatheca, adult testis.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1636.425
Larval Midgut		381.2
Larval Hindgut		803.2
Larval Malpighian Tubules		814.2
Larval Fat Body		867.3
Larval Salivary Gland		689.2
Larval Trachea		1480.8
Larval Carcass		623.55
Adult Head		1097.6
Adult Eye		1489.6
Adult Brain		1404.9
Adult Thoracic-Abdominal Ganglion		1082.3
Adult Crop		1072.4
Adult Midgut		600.5
Adult Hindgut		938.5
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		1175.9
Adult Heart		938.375
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		1145
Adult Testis		241.8
Adult Male Accessory Gland		864.9
Adult Carcass		568.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1636.425)
Larval Midgut		381.2
Larval Hindgut		(803.2)
Larval Malpighian Tubules		(814.2)
Larval Fat Body		(867.3)
Larval Salivary Gland		(689.2)
Larval Trachea		(1480.8)
Larval Carcass		(623.55)
Adult Head		(1097.6)
Adult Eye		(1489.6)
Adult Brain		(1404.9)
Adult Thoracic-Abdominal Ganglion		(1082.3)
Adult Crop		(1072.4)
Adult Midgut		(600.5)
Adult Hindgut		(938.5)
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		(1175.9)
Adult Heart		(938.375)
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		(1145)
Adult Testis		241.8
Adult Male Accessory Gland		(864.9)
Adult Carcass		(568.3)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(1636.425)
Larval Midgut		381.2
Larval Hindgut		803.2
Larval Malpighian Tubules		814.2
Larval Fat Body		867.3
Larval Salivary Gland		689.2
Larval Trachea		(1480.8)
Larval Carcass		623.55
Adult Head		(1097.6)
Adult Eye		(1489.6)
Adult Brain		(1404.9)
Adult Thoracic-Abdominal Ganglion		(1082.3)
Adult Crop		1072.4
Adult Midgut		600.5
Adult Hindgut		938.5
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		(1175.9)
Adult Heart		938.375
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		(1145)
Adult Testis		241.8
Adult Male Accessory Gland		864.9
Adult Carcass		568.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1636.425
Larval Midgut		381.2
Larval Hindgut		803.2
Larval Malpighian Tubules		814.2
Larval Fat Body		867.3
Larval Salivary Gland		689.2
Larval Trachea		1480.8
Larval Carcass		623.55
Adult Head		1097.6
Adult Eye		1489.6
Adult Brain		1404.9
Adult Thoracic-Abdominal Ganglion		1082.3
Adult Crop		1072.4
Adult Midgut		600.5
Adult Hindgut		938.5
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		1175.9
Adult Heart		938.375
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		1145
Adult Testis		241.8
Adult Male Accessory Gland		864.9
Adult Carcass		568.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1636.425
Larval Midgut		381.2
Larval Hindgut		803.2
Larval Malpighian Tubules		814.2
Larval Fat Body		867.3
Larval Salivary Gland		689.2
Larval Trachea		1480.8
Larval Carcass		623.55
Adult Head		1097.6
Adult Eye		1489.6
Adult Brain		1404.9
Adult Thoracic-Abdominal Ganglion		1082.3
Adult Crop		1072.4
Adult Midgut		600.5
Adult Hindgut		938.5
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		1175.9
Adult Heart		938.375
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		1145
Adult Testis		241.8
Adult Male Accessory Gland		864.9
Adult Carcass		568.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1636.425)
Larval Midgut		381.2
Larval Hindgut		803.2
Larval Malpighian Tubules		(814.2)
Larval Fat Body		(867.3)
Larval Salivary Gland		689.2
Larval Trachea		(1480.8)
Larval Carcass		623.55
Adult Head		(1097.6)
Adult Eye		(1489.6)
Adult Brain		(1404.9)
Adult Thoracic-Abdominal Ganglion		(1082.3)
Adult Crop		(1072.4)
Adult Midgut		600.5
Adult Hindgut		(938.5)
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		(1175.9)
Adult Heart		(938.375)
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		(1145)
Adult Testis		241.8
Adult Male Accessory Gland		(864.9)
Adult Carcass		568.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1636.425
Larval Midgut		381.2
Larval Hindgut		803.2
Larval Malpighian Tubules		814.2
Larval Fat Body		867.3
Larval Salivary Gland		689.2
Larval Trachea		1480.8
Larval Carcass		623.55
Adult Head		1097.6
Adult Eye		1489.6
Adult Brain		1404.9
Adult Thoracic-Abdominal Ganglion		1082.3
Adult Crop		1072.4
Adult Midgut		600.5
Adult Hindgut		938.5
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		1175.9
Adult Heart		938.375
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		1145
Adult Testis		241.8
Adult Male Accessory Gland		864.9
Adult Carcass		568.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1636.425
Larval Midgut		381.2
Larval Hindgut		803.2
Larval Malpighian Tubules		814.2
Larval Fat Body		867.3
Larval Salivary Gland		689.2
Larval Trachea		1480.8
Larval Carcass		623.55
Adult Head		1097.6
Adult Eye		1489.6
Adult Brain		1404.9
Adult Thoracic-Abdominal Ganglion		1082.3
Adult Crop		1072.4
Adult Midgut		600.5
Adult Hindgut		938.5
Adult Malpighian Tubules		524
Adult Fat Body		473.2
Adult Salivary Gland		1175.9
Adult Heart		938.375
Adult VirginFemale Spermatheca		487.8
Adult InseminatedFemale Spermatheca		533.6
Adult Ovary		1145
Adult Testis		241.8
Adult Male Accessory Gland		864.9
Adult Carcass		568.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	1097.6
NA	Eye	1489.6
NA	Brain	1404.9
1636.425	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	1082.3
NA	Crop	1072.4
381.2	Midgut	600.5
803.2	Hindgut	938.5
814.2	Malpighian Tubules	524
867.3	Fat Body	473.2
689.2	Salivary Gland	1175.9
NA	Heart	938.375
1480.8	Trachea	NA
NA	VirginFemale Spermatheca	487.8
NA	InseminatedFemale Spermatheca	533.6
NA	Ovary	1145
NA	Testis	241.8
NA	Male Accessory Gland	864.9
623.55	Carcass	568.3

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		318
central nervous system, larvae L3		676
central nervous system, pupae P8		529
head, virgin 1-day female		176
head, virgin 4-day female		205
head, virgin 20-day female		141
head, mated 1-day female		187
head, mated 4-day female		169
head, mated 20-day female		128
head, mated 1-day male		186
head, mated 4-day male		229
head, mated 20-day male		180
salivary gland, larvae L3 wandering		70
salivary gland, white prepupae		259
digestive system, larvae L3 wandering		172
digestive system, 1-day adult		91
digestive system, 4-day adult		91
digestive system, 20-day adult		90
fat body, larvae L3 wandering		44
fat body, white prepupae		131
fat body, pupae P8		284
carcass, larvae L3 wandering		229
carcass, 1-day adult		69
carcass, 4-day adult		83
carcass, 20-day adult		96
ovary, virgin 4-day female		166
ovary, mated 4-day female		134
testis, mated 4-day male		67
accessory gland, mated 4-day male		94
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(318)
central nervous system, larvae L3		(676)
central nervous system, pupae P8		(529)
head, virgin 1-day female		(176)
head, virgin 4-day female		(205)
head, virgin 20-day female		(141)
head, mated 1-day female		(187)
head, mated 4-day female		(169)
head, mated 20-day female		(128)
head, mated 1-day male		(186)
head, mated 4-day male		(229)
head, mated 20-day male		(180)
salivary gland, larvae L3 wandering		(70)
salivary gland, white prepupae		(259)
digestive system, larvae L3 wandering		(172)
digestive system, 1-day adult		(91)
digestive system, 4-day adult		(91)
digestive system, 20-day adult		(90)
fat body, larvae L3 wandering		(44)
fat body, white prepupae		(131)
fat body, pupae P8		(284)
carcass, larvae L3 wandering		(229)
carcass, 1-day adult		(69)
carcass, 4-day adult		(83)
carcass, 20-day adult		(96)
ovary, virgin 4-day female		(166)
ovary, mated 4-day female		(134)
testis, mated 4-day male		(67)
accessory gland, mated 4-day male		(94)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(318)
central nervous system, larvae L3		(676)
central nervous system, pupae P8		(529)
head, virgin 1-day female		(176)
head, virgin 4-day female		(205)
head, virgin 20-day female		(141)
head, mated 1-day female		(187)
head, mated 4-day female		(169)
head, mated 20-day female		(128)
head, mated 1-day male		(186)
head, mated 4-day male		(229)
head, mated 20-day male		(180)
salivary gland, larvae L3 wandering		70
salivary gland, white prepupae		(259)
digestive system, larvae L3 wandering		(172)
digestive system, 1-day adult		91
digestive system, 4-day adult		91
digestive system, 20-day adult		90
fat body, larvae L3 wandering		44
fat body, white prepupae		(131)
fat body, pupae P8		(284)
carcass, larvae L3 wandering		(229)
carcass, 1-day adult		69
carcass, 4-day adult		83
carcass, 20-day adult		96
ovary, virgin 4-day female		(166)
ovary, mated 4-day female		(134)
testis, mated 4-day male		67
accessory gland, mated 4-day male		94
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		318
central nervous system, larvae L3		676
central nervous system, pupae P8		529
head, virgin 1-day female		176
head, virgin 4-day female		205
head, virgin 20-day female		141
head, mated 1-day female		187
head, mated 4-day female		169
head, mated 20-day female		128
head, mated 1-day male		186
head, mated 4-day male		229
head, mated 20-day male		180
salivary gland, larvae L3 wandering		70
salivary gland, white prepupae		259
digestive system, larvae L3 wandering		172
digestive system, 1-day adult		91
digestive system, 4-day adult		91
digestive system, 20-day adult		90
fat body, larvae L3 wandering		44
fat body, white prepupae		131
fat body, pupae P8		284
carcass, larvae L3 wandering		229
carcass, 1-day adult		69
carcass, 4-day adult		83
carcass, 20-day adult		96
ovary, virgin 4-day female		166
ovary, mated 4-day female		134
testis, mated 4-day male		67
accessory gland, mated 4-day male		94
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		318
central nervous system, larvae L3		676
central nervous system, pupae P8		529
head, virgin 1-day female		176
head, virgin 4-day female		205
head, virgin 20-day female		141
head, mated 1-day female		187
head, mated 4-day female		169
head, mated 20-day female		128
head, mated 1-day male		186
head, mated 4-day male		229
head, mated 20-day male		180
salivary gland, larvae L3 wandering		70
salivary gland, white prepupae		259
digestive system, larvae L3 wandering		172
digestive system, 1-day adult		91
digestive system, 4-day adult		91
digestive system, 20-day adult		90
fat body, larvae L3 wandering		44
fat body, white prepupae		131
fat body, pupae P8		284
carcass, larvae L3 wandering		229
carcass, 1-day adult		69
carcass, 4-day adult		83
carcass, 20-day adult		96
ovary, virgin 4-day female		166
ovary, mated 4-day female		134
testis, mated 4-day male		67
accessory gland, mated 4-day male		94
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(318)
central nervous system, larvae L3		(676)
central nervous system, pupae P8		(529)
head, virgin 1-day female		(176)
head, virgin 4-day female		(205)
head, virgin 20-day female		(141)
head, mated 1-day female		(187)
head, mated 4-day female		(169)
head, mated 20-day female		(128)
head, mated 1-day male		(186)
head, mated 4-day male		(229)
head, mated 20-day male		(180)
salivary gland, larvae L3 wandering		(70)
salivary gland, white prepupae		(259)
digestive system, larvae L3 wandering		(172)
digestive system, 1-day adult		(91)
digestive system, 4-day adult		(91)
digestive system, 20-day adult		(90)
fat body, larvae L3 wandering		(44)
fat body, white prepupae		(131)
fat body, pupae P8		(284)
carcass, larvae L3 wandering		(229)
carcass, 1-day adult		(69)
carcass, 4-day adult		(83)
carcass, 20-day adult		(96)
ovary, virgin 4-day female		(166)
ovary, mated 4-day female		(134)
testis, mated 4-day male		(67)
accessory gland, mated 4-day male		(94)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(318)
central nervous system, larvae L3		(676)
central nervous system, pupae P8		(529)
head, virgin 1-day female		(176)
head, virgin 4-day female		(205)
head, virgin 20-day female		141
head, mated 1-day female		(187)
head, mated 4-day female		(169)
head, mated 20-day female		128
head, mated 1-day male		(186)
head, mated 4-day male		(229)
head, mated 20-day male		(180)
salivary gland, larvae L3 wandering		70
salivary gland, white prepupae		(259)
digestive system, larvae L3 wandering		(172)
digestive system, 1-day adult		91
digestive system, 4-day adult		91
digestive system, 20-day adult		90
fat body, larvae L3 wandering		44
fat body, white prepupae		131
fat body, pupae P8		(284)
carcass, larvae L3 wandering		(229)
carcass, 1-day adult		69
carcass, 4-day adult		83
carcass, 20-day adult		96
ovary, virgin 4-day female		(166)
ovary, mated 4-day female		134
testis, mated 4-day male		67
accessory gland, mated 4-day male		94
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		318
central nervous system, larvae L3		676
central nervous system, pupae P8		529
head, virgin 1-day female		176
head, virgin 4-day female		205
head, virgin 20-day female		141
head, mated 1-day female		187
head, mated 4-day female		169
head, mated 20-day female		128
head, mated 1-day male		186
head, mated 4-day male		229
head, mated 20-day male		180
salivary gland, larvae L3 wandering		70
salivary gland, white prepupae		259
digestive system, larvae L3 wandering		172
digestive system, 1-day adult		91
digestive system, 4-day adult		91
digestive system, 20-day adult		90
fat body, larvae L3 wandering		44
fat body, white prepupae		131
fat body, pupae P8		284
carcass, larvae L3 wandering		229
carcass, 1-day adult		69
carcass, 4-day adult		83
carcass, 20-day adult		96
ovary, virgin 4-day female		166
ovary, mated 4-day female		134
testis, mated 4-day male		67
accessory gland, mated 4-day male		94
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		342
embryo 02-04hr		383
embryo 04-06hr		730
embryo 06-08hr		779
embryo 08-10hr		752
embryo 10-12hr		674
embryo 12-14hr		412
embryo 14-16hr		437
embryo 16-18hr		356
embryo 18-20hr		247
embryo 20-22hr		265
embryo 22-24hr		224
larva L1		136
larva L2		127
larva L3 12hr old		93
larva L3 puffstage 1-2		124
larva L3 puffstage 3-6		202
larva L3 puffstage 7-9		241
white prepupae new		257
white prepupae 12hr		293
white prepupae 24hr		356
pupae 2d postWPP		273
pupae 3d postWPP		122
pupae 4d postWPP		94
adult male 01day		101
adult male 05day		115
adult male 30day		107
adult female 01day		192
adult female 05day		219
adult female 30day		215
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(342)
embryo 02-04hr		(383)
embryo 04-06hr		(730)
embryo 06-08hr		(779)
embryo 08-10hr		(752)
embryo 10-12hr		(674)
embryo 12-14hr		(412)
embryo 14-16hr		(437)
embryo 16-18hr		(356)
embryo 18-20hr		(247)
embryo 20-22hr		(265)
embryo 22-24hr		(224)
larva L1		(136)
larva L2		(127)
larva L3 12hr old		(93)
larva L3 puffstage 1-2		(124)
larva L3 puffstage 3-6		(202)
larva L3 puffstage 7-9		(241)
white prepupae new		(257)
white prepupae 12hr		(293)
white prepupae 24hr		(356)
pupae 2d postWPP		(273)
pupae 3d postWPP		(122)
pupae 4d postWPP		(94)
adult male 01day		(101)
adult male 05day		(115)
adult male 30day		(107)
adult female 01day		(192)
adult female 05day		(219)
adult female 30day		(215)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(342)
embryo 02-04hr		(383)
embryo 04-06hr		(730)
embryo 06-08hr		(779)
embryo 08-10hr		(752)
embryo 10-12hr		(674)
embryo 12-14hr		(412)
embryo 14-16hr		(437)
embryo 16-18hr		(356)
embryo 18-20hr		(247)
embryo 20-22hr		(265)
embryo 22-24hr		(224)
larva L1		(136)
larva L2		(127)
larva L3 12hr old		93
larva L3 puffstage 1-2		(124)
larva L3 puffstage 3-6		(202)
larva L3 puffstage 7-9		(241)
white prepupae new		(257)
white prepupae 12hr		(293)
white prepupae 24hr		(356)
pupae 2d postWPP		(273)
pupae 3d postWPP		(122)
pupae 4d postWPP		94
adult male 01day		101
adult male 05day		(115)
adult male 30day		107
adult female 01day		(192)
adult female 05day		(219)
adult female 30day		(215)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		342
embryo 02-04hr		383
embryo 04-06hr		730
embryo 06-08hr		779
embryo 08-10hr		752
embryo 10-12hr		674
embryo 12-14hr		412
embryo 14-16hr		437
embryo 16-18hr		356
embryo 18-20hr		247
embryo 20-22hr		265
embryo 22-24hr		224
larva L1		136
larva L2		127
larva L3 12hr old		93
larva L3 puffstage 1-2		124
larva L3 puffstage 3-6		202
larva L3 puffstage 7-9		241
white prepupae new		257
white prepupae 12hr		293
white prepupae 24hr		356
pupae 2d postWPP		273
pupae 3d postWPP		122
pupae 4d postWPP		94
adult male 01day		101
adult male 05day		115
adult male 30day		107
adult female 01day		192
adult female 05day		219
adult female 30day		215
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		342
embryo 02-04hr		383
embryo 04-06hr		730
embryo 06-08hr		779
embryo 08-10hr		752
embryo 10-12hr		674
embryo 12-14hr		412
embryo 14-16hr		437
embryo 16-18hr		356
embryo 18-20hr		247
embryo 20-22hr		265
embryo 22-24hr		224
larva L1		136
larva L2		127
larva L3 12hr old		93
larva L3 puffstage 1-2		124
larva L3 puffstage 3-6		202
larva L3 puffstage 7-9		241
white prepupae new		257
white prepupae 12hr		293
white prepupae 24hr		356
pupae 2d postWPP		273
pupae 3d postWPP		122
pupae 4d postWPP		94
adult male 01day		101
adult male 05day		115
adult male 30day		107
adult female 01day		192
adult female 05day		219
adult female 30day		215
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(342)
embryo 02-04hr		(383)
embryo 04-06hr		(730)
embryo 06-08hr		(779)
embryo 08-10hr		(752)
embryo 10-12hr		(674)
embryo 12-14hr		(412)
embryo 14-16hr		(437)
embryo 16-18hr		(356)
embryo 18-20hr		(247)
embryo 20-22hr		(265)
embryo 22-24hr		(224)
larva L1		(136)
larva L2		(127)
larva L3 12hr old		(93)
larva L3 puffstage 1-2		(124)
larva L3 puffstage 3-6		(202)
larva L3 puffstage 7-9		(241)
white prepupae new		(257)
white prepupae 12hr		(293)
white prepupae 24hr		(356)
pupae 2d postWPP		(273)
pupae 3d postWPP		(122)
pupae 4d postWPP		(94)
adult male 01day		(101)
adult male 05day		(115)
adult male 30day		(107)
adult female 01day		(192)
adult female 05day		(219)
adult female 30day		(215)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(342)
embryo 02-04hr		(383)
embryo 04-06hr		(730)
embryo 06-08hr		(779)
embryo 08-10hr		(752)
embryo 10-12hr		(674)
embryo 12-14hr		(412)
embryo 14-16hr		(437)
embryo 16-18hr		(356)
embryo 18-20hr		(247)
embryo 20-22hr		(265)
embryo 22-24hr		(224)
larva L1		136
larva L2		127
larva L3 12hr old		93
larva L3 puffstage 1-2		124
larva L3 puffstage 3-6		(202)
larva L3 puffstage 7-9		(241)
white prepupae new		(257)
white prepupae 12hr		(293)
white prepupae 24hr		(356)
pupae 2d postWPP		(273)
pupae 3d postWPP		122
pupae 4d postWPP		94
adult male 01day		101
adult male 05day		115
adult male 30day		107
adult female 01day		(192)
adult female 05day		(219)
adult female 30day		(215)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		342
embryo 02-04hr		383
embryo 04-06hr		730
embryo 06-08hr		779
embryo 08-10hr		752
embryo 10-12hr		674
embryo 12-14hr		412
embryo 14-16hr		437
embryo 16-18hr		356
embryo 18-20hr		247
embryo 20-22hr		265
embryo 22-24hr		224
larva L1		136
larva L2		127
larva L3 12hr old		93
larva L3 puffstage 1-2		124
larva L3 puffstage 3-6		202
larva L3 puffstage 7-9		241
white prepupae new		257
white prepupae 12hr		293
white prepupae 24hr		356
pupae 2d postWPP		273
pupae 3d postWPP		122
pupae 4d postWPP		94
adult male 01day		101
adult male 05day		115
adult male 30day		107
adult female 01day		192
adult female 05day		219
adult female 30day		215
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		500
Schneider line 2 Sg4		444
embryonic 1182-4H		212
embryonic GM2		797
embryonic Kc167		918
embryonic S1		84
embryonic S3		339
leg disc CME L1		329
wing disc CME-W2		408
wing disc ML-DmD8		624
wing disc ML-DmD9		457
wing disc ML-DmD16-c3		304
wing disc ML-DmD21		366
wing disc ML-DmD32		413
haltere disc ML-DmD17-c3		437
eye-antennal disc ML-DmD11		326
antennal disc ML-DmD20-c5		382
mixed discs ML-DmD4-c1		351
CNS ML-DmBG1-c1		306
CNS ML-DmBG2-c2		308
tumorous blood cells mbn2		312
ovary fGS/OSS		431
ovary OSC		214
ovary OSS		197
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(500)
Schneider line 2 Sg4		(444)
embryonic 1182-4H		(212)
embryonic GM2		(797)
embryonic Kc167		(918)
embryonic S1		(84)
embryonic S3		(339)
leg disc CME L1		(329)
wing disc CME-W2		(408)
wing disc ML-DmD8		(624)
wing disc ML-DmD9		(457)
wing disc ML-DmD16-c3		(304)
wing disc ML-DmD21		(366)
wing disc ML-DmD32		(413)
haltere disc ML-DmD17-c3		(437)
eye-antennal disc ML-DmD11		(326)
antennal disc ML-DmD20-c5		(382)
mixed discs ML-DmD4-c1		(351)
CNS ML-DmBG1-c1		(306)
CNS ML-DmBG2-c2		(308)
tumorous blood cells mbn2		(312)
ovary fGS/OSS		(431)
ovary OSC		(214)
ovary OSS		(197)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(500)
Schneider line 2 Sg4		(444)
embryonic 1182-4H		(212)
embryonic GM2		(797)
embryonic Kc167		(918)
embryonic S1		84
embryonic S3		(339)
leg disc CME L1		(329)
wing disc CME-W2		(408)
wing disc ML-DmD8		(624)
wing disc ML-DmD9		(457)
wing disc ML-DmD16-c3		(304)
wing disc ML-DmD21		(366)
wing disc ML-DmD32		(413)
haltere disc ML-DmD17-c3		(437)
eye-antennal disc ML-DmD11		(326)
antennal disc ML-DmD20-c5		(382)
mixed discs ML-DmD4-c1		(351)
CNS ML-DmBG1-c1		(306)
CNS ML-DmBG2-c2		(308)
tumorous blood cells mbn2		(312)
ovary fGS/OSS		(431)
ovary OSC		(214)
ovary OSS		(197)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		500
Schneider line 2 Sg4		444
embryonic 1182-4H		212
embryonic GM2		797
embryonic Kc167		918
embryonic S1		84
embryonic S3		339
leg disc CME L1		329
wing disc CME-W2		408
wing disc ML-DmD8		624
wing disc ML-DmD9		457
wing disc ML-DmD16-c3		304
wing disc ML-DmD21		366
wing disc ML-DmD32		413
haltere disc ML-DmD17-c3		437
eye-antennal disc ML-DmD11		326
antennal disc ML-DmD20-c5		382
mixed discs ML-DmD4-c1		351
CNS ML-DmBG1-c1		306
CNS ML-DmBG2-c2		308
tumorous blood cells mbn2		312
ovary fGS/OSS		431
ovary OSC		214
ovary OSS		197
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		500
Schneider line 2 Sg4		444
embryonic 1182-4H		212
embryonic GM2		797
embryonic Kc167		918
embryonic S1		84
embryonic S3		339
leg disc CME L1		329
wing disc CME-W2		408
wing disc ML-DmD8		624
wing disc ML-DmD9		457
wing disc ML-DmD16-c3		304
wing disc ML-DmD21		366
wing disc ML-DmD32		413
haltere disc ML-DmD17-c3		437
eye-antennal disc ML-DmD11		326
antennal disc ML-DmD20-c5		382
mixed discs ML-DmD4-c1		351
CNS ML-DmBG1-c1		306
CNS ML-DmBG2-c2		308
tumorous blood cells mbn2		312
ovary fGS/OSS		431
ovary OSC		214
ovary OSS		197
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(500)
Schneider line 2 Sg4		(444)
embryonic 1182-4H		(212)
embryonic GM2		(797)
embryonic Kc167		(918)
embryonic S1		(84)
embryonic S3		(339)
leg disc CME L1		(329)
wing disc CME-W2		(408)
wing disc ML-DmD8		(624)
wing disc ML-DmD9		(457)
wing disc ML-DmD16-c3		(304)
wing disc ML-DmD21		(366)
wing disc ML-DmD32		(413)
haltere disc ML-DmD17-c3		(437)
eye-antennal disc ML-DmD11		(326)
antennal disc ML-DmD20-c5		(382)
mixed discs ML-DmD4-c1		(351)
CNS ML-DmBG1-c1		(306)
CNS ML-DmBG2-c2		(308)
tumorous blood cells mbn2		(312)
ovary fGS/OSS		(431)
ovary OSC		(214)
ovary OSS		(197)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(500)
Schneider line 2 Sg4		(444)
embryonic 1182-4H		(212)
embryonic GM2		(797)
embryonic Kc167		(918)
embryonic S1		84
embryonic S3		(339)
leg disc CME L1		(329)
wing disc CME-W2		(408)
wing disc ML-DmD8		(624)
wing disc ML-DmD9		(457)
wing disc ML-DmD16-c3		(304)
wing disc ML-DmD21		(366)
wing disc ML-DmD32		(413)
haltere disc ML-DmD17-c3		(437)
eye-antennal disc ML-DmD11		(326)
antennal disc ML-DmD20-c5		(382)
mixed discs ML-DmD4-c1		(351)
CNS ML-DmBG1-c1		(306)
CNS ML-DmBG2-c2		(308)
tumorous blood cells mbn2		(312)
ovary fGS/OSS		(431)
ovary OSC		(214)
ovary OSS		(197)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		500
Schneider line 2 Sg4		444
embryonic 1182-4H		212
embryonic GM2		797
embryonic Kc167		918
embryonic S1		84
embryonic S3		339
leg disc CME L1		329
wing disc CME-W2		408
wing disc ML-DmD8		624
wing disc ML-DmD9		457
wing disc ML-DmD16-c3		304
wing disc ML-DmD21		366
wing disc ML-DmD32		413
haltere disc ML-DmD17-c3		437
eye-antennal disc ML-DmD11		326
antennal disc ML-DmD20-c5		382
mixed discs ML-DmD4-c1		351
CNS ML-DmBG1-c1		306
CNS ML-DmBG2-c2		308
tumorous blood cells mbn2		312
ovary fGS/OSS		431
ovary OSC		214
ovary OSS		197
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		82
cold shock, 4-day adult		74
heat shock, 4-day adult		110
Cadmium 50 mM 6 hrs, larvae L3		97
Cadmium 50 mM 12 hrs, larvae L3		195
Cadmium 50 mM 48 hrs, 4-day adult		93
Cadmium 100 mM 48 hrs, 4-day adult		137
Copper 0.5 mM 12 hrs, larvae L3		56
Copper 15 mM 48 hrs, 4-day adult		219
Zinc 5 mM 12 hrs, larvae L3		57
Zinc 4.5 mM 48 hrs, 4-day adult		199
Ethanol 2.5% 3 hrs, larvae L3		153
Ethanol 5% 3 hrs, larvae L3		131
Ethanol 10% 3 hrs, larvae L3		89
Caffeine 1.5 mg/ml 4 hrs, larvae L3		121
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		152
Caffeine 25 mg/ml 48 hrs, 4-day adult		74
Paraquat 5 mM 48 hrs, 4-day adult		150
Paraquat 10 mM 48 hrs, 4-day adult		122
Rotenone 2 μg 12 hrs, larvae L3		126
Rotenone 8 μg 12 hrs, larvae L3		97
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(82)
cold shock, 4-day adult		(74)
heat shock, 4-day adult		(110)
Cadmium 50 mM 6 hrs, larvae L3		(97)
Cadmium 50 mM 12 hrs, larvae L3		(195)
Cadmium 50 mM 48 hrs, 4-day adult		(93)
Cadmium 100 mM 48 hrs, 4-day adult		(137)
Copper 0.5 mM 12 hrs, larvae L3		(56)
Copper 15 mM 48 hrs, 4-day adult		(219)
Zinc 5 mM 12 hrs, larvae L3		(57)
Zinc 4.5 mM 48 hrs, 4-day adult		(199)
Ethanol 2.5% 3 hrs, larvae L3		(153)
Ethanol 5% 3 hrs, larvae L3		(131)
Ethanol 10% 3 hrs, larvae L3		(89)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(121)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(152)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(74)
Paraquat 5 mM 48 hrs, 4-day adult		(150)
Paraquat 10 mM 48 hrs, 4-day adult		(122)
Rotenone 2 μg 12 hrs, larvae L3		(126)
Rotenone 8 μg 12 hrs, larvae L3		(97)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		82
cold shock, 4-day adult		74
heat shock, 4-day adult		(110)
Cadmium 50 mM 6 hrs, larvae L3		97
Cadmium 50 mM 12 hrs, larvae L3		(195)
Cadmium 50 mM 48 hrs, 4-day adult		93
Cadmium 100 mM 48 hrs, 4-day adult		(137)
Copper 0.5 mM 12 hrs, larvae L3		56
Copper 15 mM 48 hrs, 4-day adult		(219)
Zinc 5 mM 12 hrs, larvae L3		57
Zinc 4.5 mM 48 hrs, 4-day adult		(199)
Ethanol 2.5% 3 hrs, larvae L3		(153)
Ethanol 5% 3 hrs, larvae L3		(131)
Ethanol 10% 3 hrs, larvae L3		89
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(121)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(152)
Caffeine 25 mg/ml 48 hrs, 4-day adult		74
Paraquat 5 mM 48 hrs, 4-day adult		(150)
Paraquat 10 mM 48 hrs, 4-day adult		(122)
Rotenone 2 μg 12 hrs, larvae L3		(126)
Rotenone 8 μg 12 hrs, larvae L3		97
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		82
cold shock, 4-day adult		74
heat shock, 4-day adult		110
Cadmium 50 mM 6 hrs, larvae L3		97
Cadmium 50 mM 12 hrs, larvae L3		195
Cadmium 50 mM 48 hrs, 4-day adult		93
Cadmium 100 mM 48 hrs, 4-day adult		137
Copper 0.5 mM 12 hrs, larvae L3		56
Copper 15 mM 48 hrs, 4-day adult		219
Zinc 5 mM 12 hrs, larvae L3		57
Zinc 4.5 mM 48 hrs, 4-day adult		199
Ethanol 2.5% 3 hrs, larvae L3		153
Ethanol 5% 3 hrs, larvae L3		131
Ethanol 10% 3 hrs, larvae L3		89
Caffeine 1.5 mg/ml 4 hrs, larvae L3		121
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		152
Caffeine 25 mg/ml 48 hrs, 4-day adult		74
Paraquat 5 mM 48 hrs, 4-day adult		150
Paraquat 10 mM 48 hrs, 4-day adult		122
Rotenone 2 μg 12 hrs, larvae L3		126
Rotenone 8 μg 12 hrs, larvae L3		97
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		82
cold shock, 4-day adult		74
heat shock, 4-day adult		110
Cadmium 50 mM 6 hrs, larvae L3		97
Cadmium 50 mM 12 hrs, larvae L3		195
Cadmium 50 mM 48 hrs, 4-day adult		93
Cadmium 100 mM 48 hrs, 4-day adult		137
Copper 0.5 mM 12 hrs, larvae L3		56
Copper 15 mM 48 hrs, 4-day adult		219
Zinc 5 mM 12 hrs, larvae L3		57
Zinc 4.5 mM 48 hrs, 4-day adult		199
Ethanol 2.5% 3 hrs, larvae L3		153
Ethanol 5% 3 hrs, larvae L3		131
Ethanol 10% 3 hrs, larvae L3		89
Caffeine 1.5 mg/ml 4 hrs, larvae L3		121
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		152
Caffeine 25 mg/ml 48 hrs, 4-day adult		74
Paraquat 5 mM 48 hrs, 4-day adult		150
Paraquat 10 mM 48 hrs, 4-day adult		122
Rotenone 2 μg 12 hrs, larvae L3		126
Rotenone 8 μg 12 hrs, larvae L3		97
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(82)
cold shock, 4-day adult		(74)
heat shock, 4-day adult		(110)
Cadmium 50 mM 6 hrs, larvae L3		(97)
Cadmium 50 mM 12 hrs, larvae L3		(195)
Cadmium 50 mM 48 hrs, 4-day adult		(93)
Cadmium 100 mM 48 hrs, 4-day adult		(137)
Copper 0.5 mM 12 hrs, larvae L3		(56)
Copper 15 mM 48 hrs, 4-day adult		(219)
Zinc 5 mM 12 hrs, larvae L3		(57)
Zinc 4.5 mM 48 hrs, 4-day adult		(199)
Ethanol 2.5% 3 hrs, larvae L3		(153)
Ethanol 5% 3 hrs, larvae L3		(131)
Ethanol 10% 3 hrs, larvae L3		(89)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(121)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(152)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(74)
Paraquat 5 mM 48 hrs, 4-day adult		(150)
Paraquat 10 mM 48 hrs, 4-day adult		(122)
Rotenone 2 μg 12 hrs, larvae L3		(126)
Rotenone 8 μg 12 hrs, larvae L3		(97)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		82
cold shock, 4-day adult		74
heat shock, 4-day adult		110
Cadmium 50 mM 6 hrs, larvae L3		97
Cadmium 50 mM 12 hrs, larvae L3		(195)
Cadmium 50 mM 48 hrs, 4-day adult		93
Cadmium 100 mM 48 hrs, 4-day adult		137
Copper 0.5 mM 12 hrs, larvae L3		56
Copper 15 mM 48 hrs, 4-day adult		(219)
Zinc 5 mM 12 hrs, larvae L3		57
Zinc 4.5 mM 48 hrs, 4-day adult		(199)
Ethanol 2.5% 3 hrs, larvae L3		(153)
Ethanol 5% 3 hrs, larvae L3		131
Ethanol 10% 3 hrs, larvae L3		89
Caffeine 1.5 mg/ml 4 hrs, larvae L3		121
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(152)
Caffeine 25 mg/ml 48 hrs, 4-day adult		74
Paraquat 5 mM 48 hrs, 4-day adult		(150)
Paraquat 10 mM 48 hrs, 4-day adult		122
Rotenone 2 μg 12 hrs, larvae L3		126
Rotenone 8 μg 12 hrs, larvae L3		97
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		82
cold shock, 4-day adult		74
heat shock, 4-day adult		110
Cadmium 50 mM 6 hrs, larvae L3		97
Cadmium 50 mM 12 hrs, larvae L3		195
Cadmium 50 mM 48 hrs, 4-day adult		93
Cadmium 100 mM 48 hrs, 4-day adult		137
Copper 0.5 mM 12 hrs, larvae L3		56
Copper 15 mM 48 hrs, 4-day adult		219
Zinc 5 mM 12 hrs, larvae L3		57
Zinc 4.5 mM 48 hrs, 4-day adult		199
Ethanol 2.5% 3 hrs, larvae L3		153
Ethanol 5% 3 hrs, larvae L3		131
Ethanol 10% 3 hrs, larvae L3		89
Caffeine 1.5 mg/ml 4 hrs, larvae L3		121
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		152
Caffeine 25 mg/ml 48 hrs, 4-day adult		74
Paraquat 5 mM 48 hrs, 4-day adult		150
Paraquat 10 mM 48 hrs, 4-day adult		122
Rotenone 2 μg 12 hrs, larvae L3		126
Rotenone 8 μg 12 hrs, larvae L3		97
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

mE2_34_mRNA_expression_cluster_30

External Data & Images

P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)GAL4}

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004828

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG8989-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

His3.3B^GD4749

Phenotype manifest in

Allele

Classical Alleles ( 6 )

For All Classical Alleles Show

Allele of His3.3B	Mutagen	Stocks	Known lesion
His3.3B^d04051	P-element activity	1	--
His3.3B^EP1598	P-element activity	1	--
His3.3B^NP4000		1	--
His3.3B⁰	FLPase	0	Yes
His3.3B^GG01697		0	--
His3.3B^KO	FLPase	0	Yes

Alleles Carried on Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Allele of His3.3B	Mutagen	Stocks	Known lesion
His3.3B^GD4749	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
His3.3B^HMS00148	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
His3.3B^+tHa	in vitro construct - genomic fragment	0	Yes
His3.3B^cHa	FLPase	0	Yes
His3.3B^{H3.2.T:Ivir\HA1}	FLPase	0	Yes
His3.3B^{K4A.T:Ivir\HA1}	FLPase	0	Yes
His3.3B^K4A	in vitro construct - amino acid replacement	0	Yes
His3.3B^K4R	in vitro construct - amino acid replacement	0	Yes
His3.3B^{K9A.T:Ivir\HA1}	FLPase	0	Yes
His3.3B^NIG.8989R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
His3.3B^Scer\FRT.cHa	in vitro construct	0	Yes
His3.3B^{Scer\FRT.H3.2.T:Ivir\HA1}	in vitro construct	0	Yes
His3.3B^{Scer\FRT.K4A.T:Ivir\HA1}	in vitro construct	0	Yes
His3.3B^{Scer\FRT.K9A.T:Ivir\HA1}	in vitro construct	0	Yes
His3.3B^{Scer\FRT.T:Ivir\HA1}	in vitro construct	0	Yes
His3.3B^T:Ivir\HA1	FLPase	0	Yes
His3.3B^T:Zzzz\FLAG	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Partially disrupted in

Df(1)His3.3B^KO

(Hödl and Basler, 2009)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4749}

P{NIG.8989R}

P{TRiP.HMS00148}

characterization construct

P{His3.3B.H}

P{His3.3B.K4A}

P{His3.3B.K4R}

P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.H3.2.HA}

P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.K4A.HA}

P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.K9A.HA}

P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.WT.HA}

P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.WT}

P{His3.3B(-FRT)His3.3B.H3.2.HA}

P{His3.3B(-FRT)His3.3B.K4A.HA}

P{His3.3B(-FRT)His3.3B.K9A.HA}

P{His3.3B(-FRT)His3.3B.WT.HA}

P{His3.3B(-FRT)His3.3B.WT}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}EP1356

P{EP}His3.3B^EP1598

P{EPgy2}EY00446

P{Mae-UAS.6.11}His3.3B^GG01697

P{XP}His3.3B^d04051

insertion of enhancer trap binary system

P{GawB}His3.3B^NP4000

Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )

Terms Based on Experimental Evidence ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

References

male meiosis

inferred from genetic interaction with His3.3A

(Sakai et al., 2009)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 5 terms )

Molecular Function

CV term

protein heterodimerization activity

References

DNA binding

inferred from sequence or structural similarity with NCBI_gi:484530

(FlyBase, 1992-)

inferred from electronic annotation with InterPro:IPR009072

(FlyBase Curators et al., 2004-)

Biological Process

CV term

chromatin assembly or disassembly

References

inferred from sequence or structural similarity with NCBI_gi:484530

(FlyBase, 1992-)

nucleosome assembly

inferred from electronic annotation with InterPro:IPR000164

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

(Mintseris et al., 2009.11.23)

References

nucleosome

inferred from sequence or structural similarity with NCBI_gi:484530

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

His3.3B - ATPsyn-b

anti tag coimmunoprecipitation, peptide massfingerprinting

His3.3B - Pex14

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

His3.3B - Pex5

experimental knowledge based

(Guruharsha et al., 2011)

His3.3B - RpS15Ab

experimental knowledge based

(Guruharsha et al., 2011)

His3.3B - fh

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

His3.3B - βTub85D

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

His3.3B allele

Gene

References

External Data

BioGRID - A database of protein and genetic interactions

•

58303

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0004828

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31848

Orthologs

OrthoDB Orthologs (384) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6Z6386

Dipteran inclusive ortholog search

EOG6RR6SS, EOG6VHKGW

Insect inclusive ortholog search

EOG68KQ7W

Arthropod inclusive ortholog search

EOG6KKWKK

Metazoa inclusive ortholog search

EOG6PGJW5

Orthologs in Drosophila Species (EOG6Z6386)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\His3:CG31613

Drosophila melanogaster

fruit fly

Dmel\His3:CG33824

Drosophila melanogaster

fruit fly

Dmel\His3:CG33803

Drosophila melanogaster

fruit fly

Dmel\His3:CG33860

Drosophila melanogaster

fruit fly

Dmel\His3:CG33842

Drosophila melanogaster

fruit fly

Dmel\His3.3B

Drosophila melanogaster

fruit fly

Dmel\His3:CG33830

Drosophila melanogaster

fruit fly

Dmel\His3:CG33836

Drosophila melanogaster

fruit fly

Dmel\His3:CG33857

Drosophila melanogaster

fruit fly

Dmel\His3:CG33833

Drosophila melanogaster

fruit fly

Dmel\His3:CG33821

Drosophila melanogaster

fruit fly

Dmel\His3.3A

Drosophila melanogaster

fruit fly

Dmel\His3:CG33812

Drosophila melanogaster

fruit fly

Dmel\His3:CG33815

Drosophila melanogaster

fruit fly

Dmel\His3:CG33851

Drosophila melanogaster

fruit fly

Dmel\His3:CG33848

Drosophila melanogaster

fruit fly

Dmel\His3:CG33854

Drosophila melanogaster

fruit fly

Dmel\His3:CG33845

Drosophila melanogaster

fruit fly

Dmel\His3:CG33863

Drosophila melanogaster

fruit fly

Dmel\His3:CG33806

Drosophila melanogaster

fruit fly

Dmel\His3:CG33866

Drosophila melanogaster

fruit fly

Dmel\His3:CG33839

Drosophila melanogaster

fruit fly

Dmel\His3:CG33809

Drosophila melanogaster

fruit fly

Dmel\His3:CG33818

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA22698

Drosophila pseudoobscura pseudoobscura

Dpse\GA19158

Drosophila persimilis

Dper\GL16975

Drosophila persimilis

Dper\GL22373

Drosophila persimilis

Dper\GL23951

Drosophila persimilis

Dper\GL23961

Drosophila persimilis

Dper\GL23955

Drosophila persimilis

Dper\GL26831

Drosophila persimilis

Dper\GL26550

Drosophila persimilis

Dper\GL17801

Drosophila persimilis

Dper\GL23958

Drosophila persimilis

Dper\GL19701

Drosophila willistoni

Dwil\GK12402

Drosophila willistoni

Dwil\GK19157

Drosophila willistoni

Dwil\GK19237

Drosophila willistoni

Dwil\GK23881

Drosophila willistoni

Dwil\GK25162

Drosophila willistoni

Dwil\GK25031

Drosophila willistoni

Dwil\GK21132

Drosophila willistoni

Dwil\GK18893

Drosophila willistoni

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila mojavensis

Dmoj\GI17255

Drosophila mojavensis

Dmoj\GI14879

Drosophila mojavensis

Dmoj\GI17251

Drosophila mojavensis

Dmoj\GI23438

Drosophila mojavensis

Dmoj\GI23446

Drosophila mojavensis

Dmoj\GI16893

Drosophila mojavensis

Dmoj\GI17253

Drosophila mojavensis

Dmoj\GI23442

Drosophila mojavensis

Dmoj\GI23440

Drosophila mojavensis

Dmoj\GI11258

Drosophila mojavensis

Dmoj\GI12713

Drosophila mojavensis

Dmoj\GI21876

Drosophila mojavensis

Dmoj\GI16984

Drosophila mojavensis

Dmoj\GI24857

Drosophila grimshawi

Dgri\GH11489

Drosophila grimshawi

Dgri\GH24723

Orthologs in non-Drosophila Dipterans (EOG6RR6SS, EOG6VHKGW)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Anopheles gambiae

Malaria mosquito

Agam\AGAP012709

Anopheles gambiae

Malaria mosquito

Agam\AGAP012573

Anopheles gambiae

Malaria mosquito

Agam\HIS3A

Anopheles gambiae

Malaria mosquito

Agam\AGAP005024

Anopheles gambiae

Malaria mosquito

Agam\AGAP003910

Anopheles gambiae

Malaria mosquito

Agam\AGAP012572

Anopheles gambiae

Malaria mosquito

Agam\AGAP005025

Anopheles gambiae

Malaria mosquito

Agam\AGAP012559

Orthologs in non-Dipteran Insects (EOG68KQ7W)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\LOC552116

Apis mellifera

Western honey bee

Amel\LOC552116

Apis mellifera

Western honey bee

Amel\LOC725272

Apis mellifera

Western honey bee

Amel\LOC725272

Apis mellifera

Western honey bee

Amel\LOC725272

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005050

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008914

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008993

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008913

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG036227

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG033855

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008645

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG016474

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG016479

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004948

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001064

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004960

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004954

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008995

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004745

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008672

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH20555

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH17317

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH19769

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH19771

Atta cephalotes

Leafcutter ant

Acep\ACEP26615

Atta cephalotes

Leafcutter ant

Acep\ACEP15452

Atta cephalotes

Leafcutter ant

Acep\ACEP15462

Atta cephalotes

Leafcutter ant

Acep\ACEP13016

Atta cephalotes

Leafcutter ant

Acep\ACEP26440

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17490

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17580

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO19749

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO12619

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17489

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL13534

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL13471

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL17591

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL17592

Linepithema humile

Argentine ant

Lhum\LH21289

Linepithema humile

Argentine ant

Lhum\LH24892

Linepithema humile

Argentine ant

Lhum\LH24891

Linepithema humile

Argentine ant

Lhum\LH20877

Linepithema humile

Argentine ant

Lhum\LH21967

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB16064

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB21863

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB15222

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB18138

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB18139

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB15655

Solenopsis invicta

Red fire ant

Sinv\SINV25472

Solenopsis invicta

Red fire ant

Sinv\SINV16131

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI082116

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI082897

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI085787

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000605

Acyrthosiphon pisum

Pea aphid

Acyrthosiphon pisum

Pea aphid

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI086174

Acyrthosiphon pisum

Pea aphid

Acyrthosiphon pisum

Pea aphid

Bombyx mori

Silkmoth

Bmor\BGIBMGA005550

Bombyx mori

Silkmoth

Bmor\BGIBMGA009648

Pediculus humanus

Human body louse

Phum\PHUM003560

Pediculus humanus

Human body louse

Phum\PHUM503970

Pediculus humanus

Human body louse

Phum\PHUM004150

Pediculus humanus

Human body louse

Phum\PHUM004190

Pediculus humanus

Human body louse

Phum\PHUM492720

Pediculus humanus

Human body louse

Phum\PHUM504060

Tribolium castaneum

Red flour beetle

Tcas\TC005139

Tribolium castaneum

Red flour beetle

Tcas\TC001497

Tribolium castaneum

Red flour beetle

Tcas\TC016050

Tribolium castaneum

Red flour beetle

Tcas\TC005398

Tribolium castaneum

Red flour beetle

Tcas\TC006933

Orthologs in non-Insect Arthropods (EOG6KKWKK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_43467

Daphnia pulex

Water flea

Dpux\matK

Daphnia pulex

Water flea

Dpux\NMNAT2

Daphnia pulex

Water flea

Dpux\JGI_V11_255862

Daphnia pulex

Water flea

Dpux\JGI_V11_43405

Daphnia pulex

Water flea

Dpux\bl

Daphnia pulex

Water flea

Dpux\JGI_V11_43602

Daphnia pulex

Water flea

Dpux\JGI_V11_44114

Daphnia pulex

Water flea

Dpux\JGI_V11_43863

Daphnia pulex

Water flea

Dpux\JGI_V11_43874

Daphnia pulex

Water flea

Dpux\JGI_V11_235594

Daphnia pulex

Water flea

Dpux\rbcL

Daphnia pulex

Water flea

Dpux\JGI_V11_43482

Daphnia pulex

Water flea

Dpux\JGI_V11_44116

Daphnia pulex

Water flea

Dpux\JGI_V11_235586

Daphnia pulex

Water flea

Dpux\ETH

Daphnia pulex

Water flea

Dpux\JGI_V11_43440

Daphnia pulex

Water flea

Dpux\JGI_V11_43597

Daphnia pulex

Water flea

Dpux\WASp

Daphnia pulex

Water flea

Dpux\dgt6

Daphnia pulex

Water flea

Dpux\EMC1

Daphnia pulex

Water flea

Dpux\CAND2

Daphnia pulex

Water flea

Dpux\Slc25a37

Daphnia pulex

Water flea

Dpux\JGI_V11_235620

Daphnia pulex

Water flea

Dpux\Tbc1d9

Ixodes scapularis

Deer tick

Isca\ISCW002300

Orthologs in non-Arthropod Metazoa (EOG6PGJW5)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\T10C6.13

Caenorhabditis elegans

Nematode

Cele\F45F2.13

Caenorhabditis elegans

Nematode

Cele\F17E9.10

Caenorhabditis elegans

Nematode

Cele\ZK131.2

Caenorhabditis elegans

Nematode

Cele\F54E12.1

Caenorhabditis elegans

Nematode

Cele\F07B7.5

Caenorhabditis elegans

Nematode

Cele\F08G2.3

Caenorhabditis elegans

Nematode

Cele\ZK131.3

Caenorhabditis elegans

Nematode

Cele\K06C4.13

Caenorhabditis elegans

Nematode

Cele\F55G1.2

Caenorhabditis elegans

Nematode

Cele\ZK131.7

Caenorhabditis elegans

Nematode

Cele\B0035.10

Caenorhabditis elegans

Nematode

Cele\his-71

Caenorhabditis elegans

Nematode

Cele\K06C4.5

Caenorhabditis elegans

Nematode

Cele\F22B3.2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_019208gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_018569gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_003611gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_015741gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008980gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_028816gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_015647gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_010740gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008993gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_025148gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_018526gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001711gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_014711gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_027750gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_024346gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008231gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_007820gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_003828gn

Danio rerio

Zebrafish

Drer\ENSDARG00000045248

Danio rerio

Zebrafish

Danio rerio

Zebrafish

Drer\ENSDARG00000068941

Danio rerio

Zebrafish

Drer\ENSDARG00000068436

Danio rerio

Zebrafish

Drer\ENSDARG00000068434

Danio rerio

Zebrafish

Danio rerio

Zebrafish

Danio rerio

Zebrafish

Drer\ENSDARG00000020504

Danio rerio

Zebrafish

Danio rerio

Zebrafish

Danio rerio

Zebrafish

Drer\ENSDARG00000033009

Danio rerio

Zebrafish

Danio rerio

Zebrafish

Drer\ENSDARG00000095340

Danio rerio

Zebrafish

Drer\ENSDARG00000077587

Danio rerio

Zebrafish

Drer\ENSDARG00000094154

Danio rerio

Zebrafish

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000031586

Xenopus tropicalis

Western clawed frog

Xtro\h3f3b

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000033948

Xenopus tropicalis

Western clawed frog

Xtro\h3f3b

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000031062

Xenopus tropicalis

Western clawed frog

Xtro\h3f3b

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000027843

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000030517

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000023220

Gallus gallus

Domestic chicken

Ggal\LOC417953

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000023215

Gallus gallus

Domestic chicken

Ggal\LOC417953

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000023228

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000002286

Mus musculus

House mouse

Mus musculus

House mouse

Mmus\ENSMUSG00000075032

Mus musculus

House mouse

Mmus\H3f3a

Mus musculus

House mouse

Mmus\ENSMUSG00000069265

Mus musculus

House mouse

Mus musculus

House mouse

Mmus\ENSMUSG00000093769

Mus musculus

House mouse

Mmus\ENSMUSG00000081058

Mus musculus

House mouse

Mus musculus

House mouse

Mmus\ENSMUSG00000069312

Mus musculus

House mouse

Mus musculus

House mouse

Mmus\ENSMUSG00000062417

Mus musculus

House mouse

Mus musculus

House mouse

y¹ sc^* v¹; P{TRiP.HMS00148}attP2

Mus musculus

House mouse

Mmus\ENSMUSG00000069070

Mus musculus

House mouse

Mmus\H3f3a

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000038829

Rattus norvegicus

Norway rat

Rnor\H3f3b

Rattus norvegicus

Norway rat

Rnor\Hist2h3c

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000038900

Rattus norvegicus

Norway rat

Rnor\LOC691496

Rattus norvegicus

Norway rat

Rnor\Hist2h3c

Rattus norvegicus

Norway rat

Rnor\H3f3b

Rattus norvegicus

Norway rat

Rnor\LOC100359421

Rattus norvegicus

Norway rat

Rnor\Hist2h3c

Rattus norvegicus

Norway rat

Rnor\LOC100360868

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000042585

Homo sapiens

Human

Hsap\ENSG00000124693

Homo sapiens

Human

Hsap\ENSG00000183598

Homo sapiens

Human

Hsap\ENSG00000197409

Homo sapiens

Human

Hsap\ENSG00000203813

Homo sapiens

Human

Hsap\H3F3A

Homo sapiens

Human

Hsap\ENSG00000256018

Homo sapiens

Human

Hsap\ENSG00000203852

Homo sapiens

Human

Hsap\ENSG00000182572

Homo sapiens

Human

Hsap\HIST2H3PS2

Homo sapiens

Human

Hsap\HIST3H3

Homo sapiens

Human

Hsap\ENSG00000196966

Homo sapiens

Human

Hsap\ENSG00000203811

Homo sapiens

Human

Hsap\ENSG00000198366

Homo sapiens

Human

Hsap\ENSG00000256316

Homo sapiens

Human

Hsap\ENSG00000197153

Homo sapiens

Human

Hsap\H3F3A

Homo sapiens

Human

Hsap\ENSG00000196532

Human Orthologs (17)

Gene

OMIM

HGNC

AAA Orthologs (9) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA22698

Drosophila persimilis

Dper\GL26831

Drosophila willistoni

Dwil\GK25162

Drosophila mojavensis

Dmoj\GI14879

Drosophila grimshawi

Dgri\GH24723

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

Bloomington

11274

w¹¹¹⁸ P{EP}His3.3B^EP1598

34940

Harvard

P{XP}His3.3B^d04051

Kyoto

104563

y^* w^* P{GawB}His3.3B^NP4000 / FM7c

VDRC

v12771

w¹¹¹⁸; P{GD4749}v12771

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 366 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

yak-em_His3

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0004828

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31848

Antibody Information

polyclonal

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: His3.3B CG8989

(Beasley, 1999.11)

Source for database merge of

Source for merge of: His3.3B anon-EST:fe1B6

(FlyBase, 1996-)

Additional comments

Other Comments

The canonical "H3.2" histone (His3, present in multiple copies in the genome as part of the repeating histone gene unit) and variant "His3.3" histone (encoded by His3.3A and His3.3B) forms can functionally replace each other. Cells are able to divide and differentiate when H3.2 is entirely absent but is replaced by S phase-expressed His3.3.

(Hödl and Basler, 2012)

Cells that contain a non-methylatable residue instead of K4 in all canonical and variant H3 genes are competent to respond to major developmental signaling pathways by activating target gene expression, although their proliferative capacity is slowed down relative to wild type.

(Hödl and Basler, 2012)

His3.3B has been cloned and sequenced.

The histone H3.3 replacement variant is encoded by two genes, His3.3A and His3.3B, in D.melanogaster. These genes encode exactly the same protein but are widely divergent in their untranslated regions.