Symbol Dmel\ Hrb27CSpecies D. melanogaster Name Heterogeneous nuclear ribonucleoprotein at 27C Annotation symbol CG10377 Feature type protein_coding_gene FlyBase ID FBgn0004838 Gene Model Status Current Stock availability 20 publicly
available Also Known As Hrp48, p50, RRM7, Hrb27-C Chromosome (arm) 2L Recombination map Cytogenetic map 27C4-27C4 Sequence location 2L:6,920,704..6,932,057 [+]
Genomic Maps
Automatically generated summary
The gene
Heterogeneous nuclear ribonucleoprotein at 27C is referred to in FlyBase by the symbol
Dmel\Hrb27C (CG10377, FBgn0004838). It is a protein_coding_gene from
Drosophila melanogaster . There is experimental evidence that it has the
molecular function : protein binding; mRNA 3'-UTR binding; single-stranded DNA binding. There is experimental evidence that it is involved in the
biological process : regulation of mRNA splicing, via spliceosome; border follicle cell migration; axon guidance; lateral inhibition; pole plasm oskar mRNA localization; positive regulation of translation; regulation of pole plasm oskar mRNA localization.
34 alleles are reported . The
phenotypes of these alleles are annotated with: wing margin; chorion; trichogen cell; egg; mesothoracic tergum; scutellar bristle; posterior supraalar bristle. It has
8 annotated transcripts and
8 annotated polypeptides .
Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult Malpighian tubules, adult spermathecae, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1632891_s_at partially aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of Hrb27C: 13 out of 14 probes fall into this exonic region. Gene sequence location is
2L:6920704..6932057 .
User Contributed Data
Description
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FB2013_03
FB2013_02
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FlyBase Computed Cytological Location
Experimentally Determined Cytological Location
27C4-27C5
(determined by in situ hybridisation)
27C4-27C5
(determined by in situ hybridisation)
27C-27C
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location Left of (cM) Right of (cM) Notes Comments on Gene
Model Gene model reviewed during 5.45
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated Transcripts Additional Transcript Data
& Comments Reported size (kB) Comments External Data Crossreferences Annotated
Polypeptides Additional Polypeptide
Data & Comments Reported size (kDa) 385 (aa); 48 (kD observed); 41 (kD predicted)
Comments Hrb protein was purified by RNA affinity
chromatography. It was shown to recognize specific nucleotides in a
pseudo-5' splice site within the inhibitory element of the P-element third
intron. A correction to the published sequence is presented which predicts
a larger size for the
Hrb27C protein.
hnRNP-specific mAbs were obtained and used to purify hnRNP complexes from S2 cells. hnRNP proteins were identified on the basis of nucleoplasmic localization, association with nascent transcripts, crosslinking to poly(A)-containing RNA in living cells, and amino acid sequence. Staining of polytene chromosomes reveals that
Hrb27C protein is associated with actively transcribing loci.
Hrb27C protein has two amino-terminal RNP-CS RNA-binding domains and a carboxy-terminal glycine-rich auxiliary domain.
External Data Linkouts Crossreferences
domains - A database of protein families, domains, and functional sites
DDBJ / EMBL / GenBank UniProtKB/Swiss-Prot UniProtKB/TrEMBL Mapped Features have been reorganized, please
see this article for details. Additional mapped features and mutations can
be found on GBrowse or related reports. Linkouts Crossreferences Comment: maternally deposited
Additional Descriptive Data
Marker for Subcellular Localization CV Term
Additional Descriptive Data
Hrb27C protein is detected at high levels in embryo, Kc cell, and ovary nuclear extracts. Whole-mount immunostaining shows that
Hrb27C protein is present in somatic and germ-line cells.
Marker for Subcellular Localization (GO Cellular Component) inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from physical interaction with
glo inferred from direct assay
inferred from physical interaction with
sqd Associated Tools
Reference
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
1275
Larval Midgut
688.8
Larval Hindgut
934.4
Larval Malpighian Tubules
1039.7
Larval Fat Body
1097.9
Larval Salivary Gland
1400.6
Larval Trachea
1474.175
Larval Carcass
1004.325
Adult Head
563.6
Adult Eye
561.625
Adult Brain
951.3
Adult Thoracic-Abdominal Ganglion
648.8
Adult Crop
887.9
Adult Midgut
745
Adult Hindgut
843.1
Adult Malpighian Tubules
368
Adult Fat Body
1074.5
Adult Salivary Gland
567.9
Adult Heart
970.475
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
2277
Adult Testis
244.6
Adult Male Accessory Gland
862.8
Adult Carcass
610.4
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
(1275)
Larval Midgut
(688.8)
Larval Hindgut
(934.4)
Larval Malpighian Tubules
(1039.7)
Larval Fat Body
(1097.9)
Larval Salivary Gland
(1400.6)
Larval Trachea
(1474.175)
Larval Carcass
(1004.325)
Adult Head
(563.6)
Adult Eye
(561.625)
Adult Brain
(951.3)
Adult Thoracic-Abdominal Ganglion
(648.8)
Adult Crop
(887.9)
Adult Midgut
(745)
Adult Hindgut
(843.1)
Adult Malpighian Tubules
368
Adult Fat Body
(1074.5)
Adult Salivary Gland
(567.9)
Adult Heart
(970.475)
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
(2277)
Adult Testis
244.6
Adult Male Accessory Gland
(862.8)
Adult Carcass
(610.4)
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
(1275)
Larval Midgut
688.8
Larval Hindgut
934.4
Larval Malpighian Tubules
1039.7
Larval Fat Body
(1097.9)
Larval Salivary Gland
(1400.6)
Larval Trachea
(1474.175)
Larval Carcass
1004.325
Adult Head
563.6
Adult Eye
561.625
Adult Brain
951.3
Adult Thoracic-Abdominal Ganglion
648.8
Adult Crop
887.9
Adult Midgut
745
Adult Hindgut
843.1
Adult Malpighian Tubules
368
Adult Fat Body
1074.5
Adult Salivary Gland
567.9
Adult Heart
970.475
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
(2277)
Adult Testis
244.6
Adult Male Accessory Gland
862.8
Adult Carcass
610.4
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
1275
Larval Midgut
688.8
Larval Hindgut
934.4
Larval Malpighian Tubules
1039.7
Larval Fat Body
1097.9
Larval Salivary Gland
1400.6
Larval Trachea
1474.175
Larval Carcass
1004.325
Adult Head
563.6
Adult Eye
561.625
Adult Brain
951.3
Adult Thoracic-Abdominal Ganglion
648.8
Adult Crop
887.9
Adult Midgut
745
Adult Hindgut
843.1
Adult Malpighian Tubules
368
Adult Fat Body
1074.5
Adult Salivary Gland
567.9
Adult Heart
970.475
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
2277
Adult Testis
244.6
Adult Male Accessory Gland
862.8
Adult Carcass
610.4
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
1275
Larval Midgut
688.8
Larval Hindgut
934.4
Larval Malpighian Tubules
1039.7
Larval Fat Body
1097.9
Larval Salivary Gland
1400.6
Larval Trachea
1474.175
Larval Carcass
1004.325
Adult Head
563.6
Adult Eye
561.625
Adult Brain
951.3
Adult Thoracic-Abdominal Ganglion
648.8
Adult Crop
887.9
Adult Midgut
745
Adult Hindgut
843.1
Adult Malpighian Tubules
368
Adult Fat Body
1074.5
Adult Salivary Gland
567.9
Adult Heart
970.475
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
2277
Adult Testis
244.6
Adult Male Accessory Gland
862.8
Adult Carcass
610.4
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
(1275)
Larval Midgut
688.8
Larval Hindgut
(934.4)
Larval Malpighian Tubules
(1039.7)
Larval Fat Body
(1097.9)
Larval Salivary Gland
(1400.6)
Larval Trachea
(1474.175)
Larval Carcass
(1004.325)
Adult Head
563.6
Adult Eye
561.625
Adult Brain
(951.3)
Adult Thoracic-Abdominal Ganglion
648.8
Adult Crop
(887.9)
Adult Midgut
745
Adult Hindgut
(843.1)
Adult Malpighian Tubules
368
Adult Fat Body
(1074.5)
Adult Salivary Gland
567.9
Adult Heart
(970.475)
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
(2277)
Adult Testis
244.6
Adult Male Accessory Gland
(862.8)
Adult Carcass
610.4
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
1275
Larval Midgut
688.8
Larval Hindgut
934.4
Larval Malpighian Tubules
1039.7
Larval Fat Body
1097.9
Larval Salivary Gland
1400.6
Larval Trachea
1474.175
Larval Carcass
1004.325
Adult Head
563.6
Adult Eye
561.625
Adult Brain
951.3
Adult Thoracic-Abdominal Ganglion
648.8
Adult Crop
887.9
Adult Midgut
745
Adult Hindgut
843.1
Adult Malpighian Tubules
368
Adult Fat Body
1074.5
Adult Salivary Gland
567.9
Adult Heart
970.475
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
(2277)
Adult Testis
244.6
Adult Male Accessory Gland
862.8
Adult Carcass
610.4
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
1275
Larval Midgut
688.8
Larval Hindgut
934.4
Larval Malpighian Tubules
1039.7
Larval Fat Body
1097.9
Larval Salivary Gland
1400.6
Larval Trachea
1474.175
Larval Carcass
1004.325
Adult Head
563.6
Adult Eye
561.625
Adult Brain
951.3
Adult Thoracic-Abdominal Ganglion
648.8
Adult Crop
887.9
Adult Midgut
745
Adult Hindgut
843.1
Adult Malpighian Tubules
368
Adult Fat Body
1074.5
Adult Salivary Gland
567.9
Adult Heart
970.475
Adult VirginFemale Spermatheca
196.3
Adult InseminatedFemale Spermatheca
221.7
Adult Ovary
2277
Adult Testis
244.6
Adult Male Accessory Gland
862.8
Adult Carcass
610.4
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level
Tissue
Adult Expression Level
NA Head
563.6
NA Eye
561.625
NA Brain
951.3
1275 Central Nervous System
NA
NA Thoracic-Abdominal Ganglion
648.8
NA Crop
887.9
688.8 Midgut
745
934.4 Hindgut
843.1
1039.7 Malpighian Tubules
368
1097.9 Fat Body
1074.5
1400.6 Salivary Gland
567.9
NA Heart
970.475
1474.175 Trachea
NA
NA VirginFemale Spermatheca
196.3
NA InseminatedFemale Spermatheca
221.7
NA Ovary
2277
NA Testis
244.6
NA Male Accessory Gland
862.8
1004.325 Carcass
610.4
Linear, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
215
central nervous system, larvae L3
365
central nervous system, pupae P8
252
head, virgin 1-day female
123
head, virgin 4-day female
130
head, virgin 20-day female
55
head, mated 1-day female
140
head, mated 4-day female
160
head, mated 20-day female
80
head, mated 1-day male
107
head, mated 4-day male
192
head, mated 20-day male
152
salivary gland, larvae L3 wandering
55
salivary gland, white prepupae
134
digestive system, larvae L3 wandering
146
digestive system, 1-day adult
115
digestive system, 4-day adult
220
digestive system, 20-day adult
103
fat body, larvae L3 wandering
129
fat body, white prepupae
174
fat body, pupae P8
128
carcass, larvae L3 wandering
237
carcass, 1-day adult
116
carcass, 4-day adult
161
carcass, 20-day adult
99
ovary, virgin 4-day female
358
ovary, mated 4-day female
441
testis, mated 4-day male
71
accessory gland, mated 4-day male
71
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(215)
central nervous system, larvae L3
(365)
central nervous system, pupae P8
(252)
head, virgin 1-day female
(123)
head, virgin 4-day female
(130)
head, virgin 20-day female
(55)
head, mated 1-day female
(140)
head, mated 4-day female
(160)
head, mated 20-day female
(80)
head, mated 1-day male
(107)
head, mated 4-day male
(192)
head, mated 20-day male
(152)
salivary gland, larvae L3 wandering
(55)
salivary gland, white prepupae
(134)
digestive system, larvae L3 wandering
(146)
digestive system, 1-day adult
(115)
digestive system, 4-day adult
(220)
digestive system, 20-day adult
(103)
fat body, larvae L3 wandering
(129)
fat body, white prepupae
(174)
fat body, pupae P8
(128)
carcass, larvae L3 wandering
(237)
carcass, 1-day adult
(116)
carcass, 4-day adult
(161)
carcass, 20-day adult
(99)
ovary, virgin 4-day female
(358)
ovary, mated 4-day female
(441)
testis, mated 4-day male
(71)
accessory gland, mated 4-day male
(71)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(215)
central nervous system, larvae L3
(365)
central nervous system, pupae P8
(252)
head, virgin 1-day female
(123)
head, virgin 4-day female
(130)
head, virgin 20-day female
55
head, mated 1-day female
(140)
head, mated 4-day female
(160)
head, mated 20-day female
80
head, mated 1-day male
107
head, mated 4-day male
(192)
head, mated 20-day male
(152)
salivary gland, larvae L3 wandering
55
salivary gland, white prepupae
(134)
digestive system, larvae L3 wandering
(146)
digestive system, 1-day adult
(115)
digestive system, 4-day adult
(220)
digestive system, 20-day adult
103
fat body, larvae L3 wandering
(129)
fat body, white prepupae
(174)
fat body, pupae P8
(128)
carcass, larvae L3 wandering
(237)
carcass, 1-day adult
(116)
carcass, 4-day adult
(161)
carcass, 20-day adult
99
ovary, virgin 4-day female
(358)
ovary, mated 4-day female
(441)
testis, mated 4-day male
71
accessory gland, mated 4-day male
71
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
215
central nervous system, larvae L3
365
central nervous system, pupae P8
252
head, virgin 1-day female
123
head, virgin 4-day female
130
head, virgin 20-day female
55
head, mated 1-day female
140
head, mated 4-day female
160
head, mated 20-day female
80
head, mated 1-day male
107
head, mated 4-day male
192
head, mated 20-day male
152
salivary gland, larvae L3 wandering
55
salivary gland, white prepupae
134
digestive system, larvae L3 wandering
146
digestive system, 1-day adult
115
digestive system, 4-day adult
220
digestive system, 20-day adult
103
fat body, larvae L3 wandering
129
fat body, white prepupae
174
fat body, pupae P8
128
carcass, larvae L3 wandering
237
carcass, 1-day adult
116
carcass, 4-day adult
161
carcass, 20-day adult
99
ovary, virgin 4-day female
358
ovary, mated 4-day female
441
testis, mated 4-day male
71
accessory gland, mated 4-day male
71
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
215
central nervous system, larvae L3
365
central nervous system, pupae P8
252
head, virgin 1-day female
123
head, virgin 4-day female
130
head, virgin 20-day female
55
head, mated 1-day female
140
head, mated 4-day female
160
head, mated 20-day female
80
head, mated 1-day male
107
head, mated 4-day male
192
head, mated 20-day male
152
salivary gland, larvae L3 wandering
55
salivary gland, white prepupae
134
digestive system, larvae L3 wandering
146
digestive system, 1-day adult
115
digestive system, 4-day adult
220
digestive system, 20-day adult
103
fat body, larvae L3 wandering
129
fat body, white prepupae
174
fat body, pupae P8
128
carcass, larvae L3 wandering
237
carcass, 1-day adult
116
carcass, 4-day adult
161
carcass, 20-day adult
99
ovary, virgin 4-day female
358
ovary, mated 4-day female
441
testis, mated 4-day male
71
accessory gland, mated 4-day male
71
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(215)
central nervous system, larvae L3
(365)
central nervous system, pupae P8
(252)
head, virgin 1-day female
(123)
head, virgin 4-day female
(130)
head, virgin 20-day female
(55)
head, mated 1-day female
(140)
head, mated 4-day female
(160)
head, mated 20-day female
(80)
head, mated 1-day male
(107)
head, mated 4-day male
(192)
head, mated 20-day male
(152)
salivary gland, larvae L3 wandering
(55)
salivary gland, white prepupae
(134)
digestive system, larvae L3 wandering
(146)
digestive system, 1-day adult
(115)
digestive system, 4-day adult
(220)
digestive system, 20-day adult
(103)
fat body, larvae L3 wandering
(129)
fat body, white prepupae
(174)
fat body, pupae P8
(128)
carcass, larvae L3 wandering
(237)
carcass, 1-day adult
(116)
carcass, 4-day adult
(161)
carcass, 20-day adult
(99)
ovary, virgin 4-day female
(358)
ovary, mated 4-day female
(441)
testis, mated 4-day male
(71)
accessory gland, mated 4-day male
(71)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(215)
central nervous system, larvae L3
(365)
central nervous system, pupae P8
(252)
head, virgin 1-day female
123
head, virgin 4-day female
130
head, virgin 20-day female
55
head, mated 1-day female
140
head, mated 4-day female
(160)
head, mated 20-day female
80
head, mated 1-day male
107
head, mated 4-day male
(192)
head, mated 20-day male
(152)
salivary gland, larvae L3 wandering
55
salivary gland, white prepupae
134
digestive system, larvae L3 wandering
(146)
digestive system, 1-day adult
115
digestive system, 4-day adult
(220)
digestive system, 20-day adult
103
fat body, larvae L3 wandering
129
fat body, white prepupae
(174)
fat body, pupae P8
128
carcass, larvae L3 wandering
(237)
carcass, 1-day adult
116
carcass, 4-day adult
(161)
carcass, 20-day adult
99
ovary, virgin 4-day female
(358)
ovary, mated 4-day female
(441)
testis, mated 4-day male
71
accessory gland, mated 4-day male
71
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
215
central nervous system, larvae L3
365
central nervous system, pupae P8
252
head, virgin 1-day female
123
head, virgin 4-day female
130
head, virgin 20-day female
55
head, mated 1-day female
140
head, mated 4-day female
160
head, mated 20-day female
80
head, mated 1-day male
107
head, mated 4-day male
192
head, mated 20-day male
152
salivary gland, larvae L3 wandering
55
salivary gland, white prepupae
134
digestive system, larvae L3 wandering
146
digestive system, 1-day adult
115
digestive system, 4-day adult
220
digestive system, 20-day adult
103
fat body, larvae L3 wandering
129
fat body, white prepupae
174
fat body, pupae P8
128
carcass, larvae L3 wandering
237
carcass, 1-day adult
116
carcass, 4-day adult
161
carcass, 20-day adult
99
ovary, virgin 4-day female
358
ovary, mated 4-day female
441
testis, mated 4-day male
71
accessory gland, mated 4-day male
71
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Tissue
Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male
Linear, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
135
embryo 02-04hr
181
embryo 04-06hr
184
embryo 06-08hr
177
embryo 08-10hr
156
embryo 10-12hr
122
embryo 12-14hr
119
embryo 14-16hr
62
embryo 16-18hr
59
embryo 18-20hr
39
embryo 20-22hr
54
embryo 22-24hr
58
larva L1
51
larva L2
69
larva L3 12hr old
46
larva L3 puffstage 1-2
28
larva L3 puffstage 3-6
71
larva L3 puffstage 7-9
97
white prepupae new
106
white prepupae 12hr
65
white prepupae 24hr
80
pupae 2d postWPP
65
pupae 3d postWPP
37
pupae 4d postWPP
22
adult male 01day
38
adult male 05day
36
adult male 30day
28
adult female 01day
68
adult female 05day
111
adult female 30day
105
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(135)
embryo 02-04hr
(181)
embryo 04-06hr
(184)
embryo 06-08hr
(177)
embryo 08-10hr
(156)
embryo 10-12hr
(122)
embryo 12-14hr
(119)
embryo 14-16hr
(62)
embryo 16-18hr
(59)
embryo 18-20hr
(39)
embryo 20-22hr
(54)
embryo 22-24hr
(58)
larva L1
(51)
larva L2
(69)
larva L3 12hr old
(46)
larva L3 puffstage 1-2
(28)
larva L3 puffstage 3-6
(71)
larva L3 puffstage 7-9
(97)
white prepupae new
(106)
white prepupae 12hr
(65)
white prepupae 24hr
(80)
pupae 2d postWPP
(65)
pupae 3d postWPP
(37)
pupae 4d postWPP
22
adult male 01day
(38)
adult male 05day
(36)
adult male 30day
(28)
adult female 01day
(68)
adult female 05day
(111)
adult female 30day
(105)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
(135)
embryo 02-04hr
(181)
embryo 04-06hr
(184)
embryo 06-08hr
(177)
embryo 08-10hr
(156)
embryo 10-12hr
(122)
embryo 12-14hr
(119)
embryo 14-16hr
62
embryo 16-18hr
59
embryo 18-20hr
39
embryo 20-22hr
54
embryo 22-24hr
58
larva L1
51
larva L2
69
larva L3 12hr old
46
larva L3 puffstage 1-2
28
larva L3 puffstage 3-6
71
larva L3 puffstage 7-9
97
white prepupae new
106
white prepupae 12hr
65
white prepupae 24hr
80
pupae 2d postWPP
65
pupae 3d postWPP
37
pupae 4d postWPP
22
adult male 01day
38
adult male 05day
36
adult male 30day
28
adult female 01day
68
adult female 05day
(111)
adult female 30day
105
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
135
embryo 02-04hr
181
embryo 04-06hr
184
embryo 06-08hr
177
embryo 08-10hr
156
embryo 10-12hr
122
embryo 12-14hr
119
embryo 14-16hr
62
embryo 16-18hr
59
embryo 18-20hr
39
embryo 20-22hr
54
embryo 22-24hr
58
larva L1
51
larva L2
69
larva L3 12hr old
46
larva L3 puffstage 1-2
28
larva L3 puffstage 3-6
71
larva L3 puffstage 7-9
97
white prepupae new
106
white prepupae 12hr
65
white prepupae 24hr
80
pupae 2d postWPP
65
pupae 3d postWPP
37
pupae 4d postWPP
22
adult male 01day
38
adult male 05day
36
adult male 30day
28
adult female 01day
68
adult female 05day
111
adult female 30day
105
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
135
embryo 02-04hr
181
embryo 04-06hr
184
embryo 06-08hr
177
embryo 08-10hr
156
embryo 10-12hr
122
embryo 12-14hr
119
embryo 14-16hr
62
embryo 16-18hr
59
embryo 18-20hr
39
embryo 20-22hr
54
embryo 22-24hr
58
larva L1
51
larva L2
69
larva L3 12hr old
46
larva L3 puffstage 1-2
28
larva L3 puffstage 3-6
71
larva L3 puffstage 7-9
97
white prepupae new
106
white prepupae 12hr
65
white prepupae 24hr
80
pupae 2d postWPP
65
pupae 3d postWPP
37
pupae 4d postWPP
22
adult male 01day
38
adult male 05day
36
adult male 30day
28
adult female 01day
68
adult female 05day
111
adult female 30day
105
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(135)
embryo 02-04hr
(181)
embryo 04-06hr
(184)
embryo 06-08hr
(177)
embryo 08-10hr
(156)
embryo 10-12hr
(122)
embryo 12-14hr
(119)
embryo 14-16hr
(62)
embryo 16-18hr
(59)
embryo 18-20hr
(39)
embryo 20-22hr
(54)
embryo 22-24hr
(58)
larva L1
(51)
larva L2
(69)
larva L3 12hr old
(46)
larva L3 puffstage 1-2
28
larva L3 puffstage 3-6
(71)
larva L3 puffstage 7-9
(97)
white prepupae new
(106)
white prepupae 12hr
(65)
white prepupae 24hr
(80)
pupae 2d postWPP
(65)
pupae 3d postWPP
(37)
pupae 4d postWPP
22
adult male 01day
(38)
adult male 05day
(36)
adult male 30day
28
adult female 01day
(68)
adult female 05day
(111)
adult female 30day
(105)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
135
embryo 02-04hr
(181)
embryo 04-06hr
(184)
embryo 06-08hr
(177)
embryo 08-10hr
(156)
embryo 10-12hr
122
embryo 12-14hr
119
embryo 14-16hr
62
embryo 16-18hr
59
embryo 18-20hr
39
embryo 20-22hr
54
embryo 22-24hr
58
larva L1
51
larva L2
69
larva L3 12hr old
46
larva L3 puffstage 1-2
28
larva L3 puffstage 3-6
71
larva L3 puffstage 7-9
97
white prepupae new
106
white prepupae 12hr
65
white prepupae 24hr
80
pupae 2d postWPP
65
pupae 3d postWPP
37
pupae 4d postWPP
22
adult male 01day
38
adult male 05day
36
adult male 30day
28
adult female 01day
68
adult female 05day
111
adult female 30day
105
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
135
embryo 02-04hr
181
embryo 04-06hr
184
embryo 06-08hr
177
embryo 08-10hr
156
embryo 10-12hr
122
embryo 12-14hr
119
embryo 14-16hr
62
embryo 16-18hr
59
embryo 18-20hr
39
embryo 20-22hr
54
embryo 22-24hr
58
larva L1
51
larva L2
69
larva L3 12hr old
46
larva L3 puffstage 1-2
28
larva L3 puffstage 3-6
71
larva L3 puffstage 7-9
97
white prepupae new
106
white prepupae 12hr
65
white prepupae 24hr
80
pupae 2d postWPP
65
pupae 3d postWPP
37
pupae 4d postWPP
22
adult male 01day
38
adult male 05day
36
adult male 30day
28
adult female 01day
68
adult female 05day
111
adult female 30day
105
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
Linear, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
259
Schneider line 2 Sg4
360
embryonic 1182-4H
251
embryonic GM2
341
embryonic Kc167
633
embryonic S1
380
embryonic S3
514
leg disc CME L1
314
wing disc CME-W2
427
wing disc ML-DmD8
354
wing disc ML-DmD9
228
wing disc ML-DmD16-c3
711
wing disc ML-DmD21
332
wing disc ML-DmD32
572
haltere disc ML-DmD17-c3
256
eye-antennal disc ML-DmD11
256
antennal disc ML-DmD20-c5
336
mixed discs ML-DmD4-c1
494
CNS ML-DmBG1-c1
454
CNS ML-DmBG2-c2
272
tumorous blood cells mbn2
182
ovary fGS/OSS
522
ovary OSC
906
ovary OSS
783
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(259)
Schneider line 2 Sg4
(360)
embryonic 1182-4H
(251)
embryonic GM2
(341)
embryonic Kc167
(633)
embryonic S1
(380)
embryonic S3
(514)
leg disc CME L1
(314)
wing disc CME-W2
(427)
wing disc ML-DmD8
(354)
wing disc ML-DmD9
(228)
wing disc ML-DmD16-c3
(711)
wing disc ML-DmD21
(332)
wing disc ML-DmD32
(572)
haltere disc ML-DmD17-c3
(256)
eye-antennal disc ML-DmD11
(256)
antennal disc ML-DmD20-c5
(336)
mixed discs ML-DmD4-c1
(494)
CNS ML-DmBG1-c1
(454)
CNS ML-DmBG2-c2
(272)
tumorous blood cells mbn2
(182)
ovary fGS/OSS
(522)
ovary OSC
(906)
ovary OSS
(783)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
(259)
Schneider line 2 Sg4
(360)
embryonic 1182-4H
(251)
embryonic GM2
(341)
embryonic Kc167
(633)
embryonic S1
(380)
embryonic S3
(514)
leg disc CME L1
(314)
wing disc CME-W2
(427)
wing disc ML-DmD8
(354)
wing disc ML-DmD9
(228)
wing disc ML-DmD16-c3
(711)
wing disc ML-DmD21
(332)
wing disc ML-DmD32
(572)
haltere disc ML-DmD17-c3
(256)
eye-antennal disc ML-DmD11
(256)
antennal disc ML-DmD20-c5
(336)
mixed discs ML-DmD4-c1
(494)
CNS ML-DmBG1-c1
(454)
CNS ML-DmBG2-c2
(272)
tumorous blood cells mbn2
(182)
ovary fGS/OSS
(522)
ovary OSC
(906)
ovary OSS
(783)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
259
Schneider line 2 Sg4
360
embryonic 1182-4H
251
embryonic GM2
341
embryonic Kc167
633
embryonic S1
380
embryonic S3
514
leg disc CME L1
314
wing disc CME-W2
427
wing disc ML-DmD8
354
wing disc ML-DmD9
228
wing disc ML-DmD16-c3
711
wing disc ML-DmD21
332
wing disc ML-DmD32
572
haltere disc ML-DmD17-c3
256
eye-antennal disc ML-DmD11
256
antennal disc ML-DmD20-c5
336
mixed discs ML-DmD4-c1
494
CNS ML-DmBG1-c1
454
CNS ML-DmBG2-c2
272
tumorous blood cells mbn2
182
ovary fGS/OSS
522
ovary OSC
906
ovary OSS
783
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
259
Schneider line 2 Sg4
360
embryonic 1182-4H
251
embryonic GM2
341
embryonic Kc167
633
embryonic S1
380
embryonic S3
514
leg disc CME L1
314
wing disc CME-W2
427
wing disc ML-DmD8
354
wing disc ML-DmD9
228
wing disc ML-DmD16-c3
711
wing disc ML-DmD21
332
wing disc ML-DmD32
572
haltere disc ML-DmD17-c3
256
eye-antennal disc ML-DmD11
256
antennal disc ML-DmD20-c5
336
mixed discs ML-DmD4-c1
494
CNS ML-DmBG1-c1
454
CNS ML-DmBG2-c2
272
tumorous blood cells mbn2
182
ovary fGS/OSS
522
ovary OSC
906
ovary OSS
783
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
log, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(259)
Schneider line 2 Sg4
(360)
embryonic 1182-4H
(251)
embryonic GM2
(341)
embryonic Kc167
(633)
embryonic S1
(380)
embryonic S3
(514)
leg disc CME L1
(314)
wing disc CME-W2
(427)
wing disc ML-DmD8
(354)
wing disc ML-DmD9
(228)
wing disc ML-DmD16-c3
(711)
wing disc ML-DmD21
(332)
wing disc ML-DmD32
(572)
haltere disc ML-DmD17-c3
(256)
eye-antennal disc ML-DmD11
(256)
antennal disc ML-DmD20-c5
(336)
mixed discs ML-DmD4-c1
(494)
CNS ML-DmBG1-c1
(454)
CNS ML-DmBG2-c2
(272)
tumorous blood cells mbn2
(182)
ovary fGS/OSS
(522)
ovary OSC
(906)
ovary OSS
(783)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
(259)
Schneider line 2 Sg4
(360)
embryonic 1182-4H
(251)
embryonic GM2
(341)
embryonic Kc167
(633)
embryonic S1
(380)
embryonic S3
(514)
leg disc CME L1
(314)
wing disc CME-W2
(427)
wing disc ML-DmD8
(354)
wing disc ML-DmD9
(228)
wing disc ML-DmD16-c3
(711)
wing disc ML-DmD21
(332)
wing disc ML-DmD32
(572)
haltere disc ML-DmD17-c3
(256)
eye-antennal disc ML-DmD11
(256)
antennal disc ML-DmD20-c5
(336)
mixed discs ML-DmD4-c1
(494)
CNS ML-DmBG1-c1
(454)
CNS ML-DmBG2-c2
(272)
tumorous blood cells mbn2
(182)
ovary fGS/OSS
(522)
ovary OSC
(906)
ovary OSS
(783)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
259
Schneider line 2 Sg4
360
embryonic 1182-4H
251
embryonic GM2
341
embryonic Kc167
633
embryonic S1
380
embryonic S3
514
leg disc CME L1
314
wing disc CME-W2
427
wing disc ML-DmD8
354
wing disc ML-DmD9
228
wing disc ML-DmD16-c3
711
wing disc ML-DmD21
332
wing disc ML-DmD32
572
haltere disc ML-DmD17-c3
256
eye-antennal disc ML-DmD11
256
antennal disc ML-DmD20-c5
336
mixed discs ML-DmD4-c1
494
CNS ML-DmBG1-c1
454
CNS ML-DmBG2-c2
272
tumorous blood cells mbn2
182
ovary fGS/OSS
522
ovary OSC
906
ovary OSS
783
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Cell Line
Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS
Linear, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
164
cold shock, 4-day adult
110
heat shock, 4-day adult
164
Cadmium 50 mM 6 hrs, larvae L3
74
Cadmium 50 mM 12 hrs, larvae L3
99
Cadmium 50 mM 48 hrs, 4-day adult
174
Cadmium 100 mM 48 hrs, 4-day adult
344
Copper 0.5 mM 12 hrs, larvae L3
218
Copper 15 mM 48 hrs, 4-day adult
705
Zinc 5 mM 12 hrs, larvae L3
115
Zinc 4.5 mM 48 hrs, 4-day adult
322
Ethanol 2.5% 3 hrs, larvae L3
170
Ethanol 5% 3 hrs, larvae L3
73
Ethanol 10% 3 hrs, larvae L3
79
Caffeine 1.5 mg/ml 4 hrs, larvae L3
61
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
290
Caffeine 25 mg/ml 48 hrs, 4-day adult
237
Paraquat 5 mM 48 hrs, 4-day adult
230
Paraquat 10 mM 48 hrs, 4-day adult
164
Rotenone 2 μg 12 hrs, larvae L3
72
Rotenone 8 μg 12 hrs, larvae L3
44
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
(164)
cold shock, 4-day adult
(110)
heat shock, 4-day adult
(164)
Cadmium 50 mM 6 hrs, larvae L3
(74)
Cadmium 50 mM 12 hrs, larvae L3
(99)
Cadmium 50 mM 48 hrs, 4-day adult
(174)
Cadmium 100 mM 48 hrs, 4-day adult
(344)
Copper 0.5 mM 12 hrs, larvae L3
(218)
Copper 15 mM 48 hrs, 4-day adult
(705)
Zinc 5 mM 12 hrs, larvae L3
(115)
Zinc 4.5 mM 48 hrs, 4-day adult
(322)
Ethanol 2.5% 3 hrs, larvae L3
(170)
Ethanol 5% 3 hrs, larvae L3
(73)
Ethanol 10% 3 hrs, larvae L3
(79)
Caffeine 1.5 mg/ml 4 hrs, larvae L3
(61)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(290)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(237)
Paraquat 5 mM 48 hrs, 4-day adult
(230)
Paraquat 10 mM 48 hrs, 4-day adult
(164)
Rotenone 2 μg 12 hrs, larvae L3
(72)
Rotenone 8 μg 12 hrs, larvae L3
(44)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
(164)
cold shock, 4-day adult
(110)
heat shock, 4-day adult
(164)
Cadmium 50 mM 6 hrs, larvae L3
74
Cadmium 50 mM 12 hrs, larvae L3
99
Cadmium 50 mM 48 hrs, 4-day adult
(174)
Cadmium 100 mM 48 hrs, 4-day adult
(344)
Copper 0.5 mM 12 hrs, larvae L3
(218)
Copper 15 mM 48 hrs, 4-day adult
(705)
Zinc 5 mM 12 hrs, larvae L3
(115)
Zinc 4.5 mM 48 hrs, 4-day adult
(322)
Ethanol 2.5% 3 hrs, larvae L3
(170)
Ethanol 5% 3 hrs, larvae L3
73
Ethanol 10% 3 hrs, larvae L3
79
Caffeine 1.5 mg/ml 4 hrs, larvae L3
61
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(290)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(237)
Paraquat 5 mM 48 hrs, 4-day adult
(230)
Paraquat 10 mM 48 hrs, 4-day adult
(164)
Rotenone 2 μg 12 hrs, larvae L3
72
Rotenone 8 μg 12 hrs, larvae L3
44
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
164
cold shock, 4-day adult
110
heat shock, 4-day adult
164
Cadmium 50 mM 6 hrs, larvae L3
74
Cadmium 50 mM 12 hrs, larvae L3
99
Cadmium 50 mM 48 hrs, 4-day adult
174
Cadmium 100 mM 48 hrs, 4-day adult
344
Copper 0.5 mM 12 hrs, larvae L3
218
Copper 15 mM 48 hrs, 4-day adult
705
Zinc 5 mM 12 hrs, larvae L3
115
Zinc 4.5 mM 48 hrs, 4-day adult
322
Ethanol 2.5% 3 hrs, larvae L3
170
Ethanol 5% 3 hrs, larvae L3
73
Ethanol 10% 3 hrs, larvae L3
79
Caffeine 1.5 mg/ml 4 hrs, larvae L3
61
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
290
Caffeine 25 mg/ml 48 hrs, 4-day adult
237
Paraquat 5 mM 48 hrs, 4-day adult
230
Paraquat 10 mM 48 hrs, 4-day adult
164
Rotenone 2 μg 12 hrs, larvae L3
72
Rotenone 8 μg 12 hrs, larvae L3
44
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
164
cold shock, 4-day adult
110
heat shock, 4-day adult
164
Cadmium 50 mM 6 hrs, larvae L3
74
Cadmium 50 mM 12 hrs, larvae L3
99
Cadmium 50 mM 48 hrs, 4-day adult
174
Cadmium 100 mM 48 hrs, 4-day adult
344
Copper 0.5 mM 12 hrs, larvae L3
218
Copper 15 mM 48 hrs, 4-day adult
705
Zinc 5 mM 12 hrs, larvae L3
115
Zinc 4.5 mM 48 hrs, 4-day adult
322
Ethanol 2.5% 3 hrs, larvae L3
170
Ethanol 5% 3 hrs, larvae L3
73
Ethanol 10% 3 hrs, larvae L3
79
Caffeine 1.5 mg/ml 4 hrs, larvae L3
61
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
290
Caffeine 25 mg/ml 48 hrs, 4-day adult
237
Paraquat 5 mM 48 hrs, 4-day adult
230
Paraquat 10 mM 48 hrs, 4-day adult
164
Rotenone 2 μg 12 hrs, larvae L3
72
Rotenone 8 μg 12 hrs, larvae L3
44
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
log, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
(164)
cold shock, 4-day adult
(110)
heat shock, 4-day adult
(164)
Cadmium 50 mM 6 hrs, larvae L3
(74)
Cadmium 50 mM 12 hrs, larvae L3
(99)
Cadmium 50 mM 48 hrs, 4-day adult
(174)
Cadmium 100 mM 48 hrs, 4-day adult
(344)
Copper 0.5 mM 12 hrs, larvae L3
(218)
Copper 15 mM 48 hrs, 4-day adult
(705)
Zinc 5 mM 12 hrs, larvae L3
(115)
Zinc 4.5 mM 48 hrs, 4-day adult
(322)
Ethanol 2.5% 3 hrs, larvae L3
(170)
Ethanol 5% 3 hrs, larvae L3
(73)
Ethanol 10% 3 hrs, larvae L3
(79)
Caffeine 1.5 mg/ml 4 hrs, larvae L3
(61)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(290)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(237)
Paraquat 5 mM 48 hrs, 4-day adult
(230)
Paraquat 10 mM 48 hrs, 4-day adult
(164)
Rotenone 2 μg 12 hrs, larvae L3
(72)
Rotenone 8 μg 12 hrs, larvae L3
(44)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
(164)
cold shock, 4-day adult
110
heat shock, 4-day adult
(164)
Cadmium 50 mM 6 hrs, larvae L3
74
Cadmium 50 mM 12 hrs, larvae L3
99
Cadmium 50 mM 48 hrs, 4-day adult
(174)
Cadmium 100 mM 48 hrs, 4-day adult
(344)
Copper 0.5 mM 12 hrs, larvae L3
(218)
Copper 15 mM 48 hrs, 4-day adult
(705)
Zinc 5 mM 12 hrs, larvae L3
115
Zinc 4.5 mM 48 hrs, 4-day adult
(322)
Ethanol 2.5% 3 hrs, larvae L3
(170)
Ethanol 5% 3 hrs, larvae L3
73
Ethanol 10% 3 hrs, larvae L3
79
Caffeine 1.5 mg/ml 4 hrs, larvae L3
61
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(290)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(237)
Paraquat 5 mM 48 hrs, 4-day adult
(230)
Paraquat 10 mM 48 hrs, 4-day adult
(164)
Rotenone 2 μg 12 hrs, larvae L3
72
Rotenone 8 μg 12 hrs, larvae L3
44
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
164
cold shock, 4-day adult
110
heat shock, 4-day adult
164
Cadmium 50 mM 6 hrs, larvae L3
74
Cadmium 50 mM 12 hrs, larvae L3
99
Cadmium 50 mM 48 hrs, 4-day adult
174
Cadmium 100 mM 48 hrs, 4-day adult
344
Copper 0.5 mM 12 hrs, larvae L3
218
Copper 15 mM 48 hrs, 4-day adult
705
Zinc 5 mM 12 hrs, larvae L3
115
Zinc 4.5 mM 48 hrs, 4-day adult
322
Ethanol 2.5% 3 hrs, larvae L3
170
Ethanol 5% 3 hrs, larvae L3
73
Ethanol 10% 3 hrs, larvae L3
79
Caffeine 1.5 mg/ml 4 hrs, larvae L3
61
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
290
Caffeine 25 mg/ml 48 hrs, 4-day adult
237
Paraquat 5 mM 48 hrs, 4-day adult
230
Paraquat 10 mM 48 hrs, 4-day adult
164
Rotenone 2 μg 12 hrs, larvae L3
72
Rotenone 8 μg 12 hrs, larvae L3
44
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Treatment
Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3
A cluster of genes with similar mRNA expression dynamics across development.
Linkouts
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
nurse cell & nuclear chromosome (with
Hrb27C377 )
oocyte & cytoskeleton | germ-line clone
For All Classical Alleles Show For All Alleles Carried on Transgenic
Constructs Show Disrupted in Not disrupted in Transgenic Constructs characterization construct
Insertions insertion of mobile activating element
insertion of enhancer trap
Molecular Function inferred from direct assay
inferred from physical interaction with
glo inferred from direct assay
Biological Process inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
Cellular Component inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from physical interaction with
glo inferred from direct assay
inferred from physical interaction with
sqd Molecular Function traceable author statement
non-traceable author statement
traceable author statement
inferred from sequence or structural similarity
non-traceable author statement
Biological Process traceable author statement
Cellular Component inferred from sequence or structural similarity with
human HNRPA1 non-traceable author statement
protein-protein experimental knowledge based
experimental knowledge based
Interacts with Please look at the allele data
for full details of the genetic interactions
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
OrthoDB Ortholog Groups
OrthoDB orthology group searches
Drosophila inclusive ortholog search
Dipteran inclusive ortholog search
Insect inclusive ortholog search
Arthropod inclusive ortholog search
Metazoa inclusive ortholog search
EOG6N3180 )
Drosophila melanogaster
fruit fly
Drosophila pseudoobscura pseudoobscura
Y
EOG6ZCTDQ )
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
EOG6NK9R8 )
Apis mellifera
Western honey bee
Amel\GB40057
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG024987
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH27139
Atta cephalotes
Leafcutter ant
Acep\ACEP24794
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO22242
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL15834
Linepithema humile
Argentine ant
Lhum\LH21695
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB13211
Solenopsis invicta
Red fire ant
Sinv\SINV24865
Acyrthosiphon pisum
Pea aphid
Pediculus humanus
Human body louse
Tribolium castaneum
Red flour beetle
EOG6NZS8W )
Ixodes scapularis
Deer tick
EOG6G20CW )
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
AAA Orthologs (11)
based on analysis using Dmel annotation version 4.3
Drosophila pseudoobscura pseudoobscura
Bloomington Harvard Kyoto VDRC Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
Please Note This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see
GBrowse for alignment of the cDNAs and ESTs
to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones Other clones cDNA Clones, End Sequenced
(ESTs) BDGP DGC clones Other clones Linkouts
- Results from RNAi screens.
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Source for database identity of Source for database merge of Source for merge of: Hrb27C linha
Source for merge of: Hrb27C p50
Source for merge of: Hrb27C Rbp7
Additional comments RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells in the presence of
Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without
Cdc27 dsRNA.
Hrb27C mutants disrupt the formation of particles in the oocyte cytoplasm containing
stau protein.
In germline clones mutant for
Hrb27C ,
osk mRNA and
stau fail to localise to the posterior of the oocyte, although
bcd mRNA localises normally at the anterior.
Identification: in a germline clone screen to identify genes involved in localisation of the
bcd ,
osk and
grk mRNAs.
Hrb27C is required for a novel step in the localisation of
osk mRNA.
Hrb27C plays a critical role in the inclusion of mI and mII microexons in
Ubx mRNAs.
Identified as a 50kD protein that binds to a discrete translational derepressor element located at the 5' end of the
osk transcript.
Hrb27C protein also binds to the Bruno-response element (BRE) at the 3' end of the
osk transcript.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Hrb27C plays a functional role in
P\T IVS3 splicing inhibition.
Sxl does not form an immunoprecipitable complex with
Hrb27C .
Psi is a soma-specific alternative splicing factor that functions with general factors, including
Hrb27C (a 50kD RNA-binding protein), to regulate germ line specific
P-element expression.
Hrb27C binds specifically to intron 3 (IVS3) 5' exon splice site.
A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs.
The distribution of the hnRNP protein
Hrb27C on nascent transcripts from polytene chromosomes has been studied.
Protein has been isolated by its ability to bind single-stranded DNA.
An
Hrb27C cDNA clone has been isolated and sequenced.
Biochemical experiments support a mechanism for somatic inhibition of
P-element intron 3 (IVS3) splicing in which the binding of a U1snRNP and multiprotein complexes to two exon pseudo 5' splice sites (F1 and F2) prevents U1snRNP binding to the accurate 5' splice site. A 50kD protein,
Hrb27C contacts the F2 5' splice site.
Sequence Crossreferences
- A searchable database of RefSeq genes.
Other Crossreferences
domains - A database of protein families, domains, and functional sites
- Patterns of gene expression in Drosophila embryogenesis
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
- Results from RNAi screens.
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
- Integrated genomics database for Drosophila, Anopheles, and C.elegans
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- Data generated by the modENCODE project.
Reported As Symbol Synonym CG10377
(
Jin et al., 2001 ,
Quinones-Coello, 2007 ,
Goshima et al., 2007 ,
Grieder et al., 2007 ,
Jagadeeshan and Singh, 2005 ,
Mohammad et al., 2009 ,
Keleman et al., 2009.8.5 ,
Berger et al., 2008 ,
Ni et al., 2008 ,
Ritson et al., 2010 ,
Ni et al., 2010.12.1 )
Hrb27C
(
St Johnston, 2006.10.4 ,
McDermott et al., 2012 ,
Quinones-Coello, 2007 ,
White et al., 2011 ,
Nelson et al., 2007 ,
Minidorff et al., 2007 ,
Baxley et al., 2011 ,
Hartmann et al., 2011 ,
Kallappagoudar et al., 2010 ,
Snee and Macdonald, 2009 ,
Nie et al., 2009 ,
Chew et al., 2009 ,
Klusza and Deng, 2010 ,
Ritson et al., 2010 ,
Franco et al., 2011 ,
Olson et al., 2007 ,
Berger et al., 2012 ,
Japanese National Institute of Genetics, 2012.5.21 )
Hrp48
(
Minakhina and Steward, 2005 ,
Hazelrigg, 2004 ,
Labourier et al., 2001 ,
Castagnetti and Ephrussi, 2003 ,
Geng and Macdonald, 2006 ,
Hartmann et al., 2011 ,
Besse et al., 2009 ,
Mhlanga et al., 2009 ,
Suissa et al., 2010 )
hrp48
(
Blanchette et al., 2005 ,
Yano et al., 2004 ,
Smith and Valcarcel, 2000 ,
Vorbruggen et al., 2000 ,
Vorbruggen et al., 2000 ,
Burnette et al., 1999 ,
Lopez et al., 1999 ,
Burnette et al., 1999 ,
Burnette et al., 1999 ,
Burnette et al., 1998 ,
Labrador and Corces, 1997 ,
Hammond et al., 1997 ,
Warsowe and Rio, 1997 ,
Deshpande et al., 1996 ,
Siebel et al., 1995 ,
Siebel et al., 1994 ,
Samuels et al., 1994 ,
Weeks et al., 1993 ,
Matunis et al., 1993 ,
Matunis et al., 1992 ,
Matunis et al., 1992 ,
Blencowe, 2006 ,
St Johnston, 2006.10.4 ,
Mathieu et al., 2007 ,
Zimyanin et al., 2008 ,
Blanchette et al., 2009 ,
Kalifa et al., 2009 ,
Amarasinghe et al., 2001 )
p50
(
Huynh et al., 2004 ,
Wilkie et al., 2003 ,
Mahowald, 2001 ,
Macdonald, 2001 ,
Lipshitz and Smibert, 2000 ,
Johnstone and Lasko, 2001 ,
Castagnetti et al., 2000 ,
Gunkel et al., 1998 , )
Name Synonym Heterogeneous nuclear ribonucleoprotein
Heterogeneous nuclear ribonucleoprotein at 27C
Secondary FlyBase
IDs FBgn0010261 FBgn0016665 FBgn0019729 FBgn0024845 FBgn0061493 Generate a list of List References by type
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. [FBrf0219320] Effectors of alcohol-induced cell killing in Drosophila. [FBrf0219410] ELAV mediates 3' UTR extension in the Drosophila nervous system. [FBrf0219699] Long-term and short-term evolutionary impacts of transposable elements on Drosophila. [FBrf0220133] Drosophila Syncrip binds the gurken mRNA localisation signal and regulates localised transcripts during axis specification. [FBrf0218722] Global Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila. [FBrf0218523] Drosophila patterning is established by differential association of mRNAs with P bodies. [FBrf0220085] The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis. [FBrf0214454] A Novel Strategy to Isolate Ubiquitin Conjugates Reveals Wide Role for Ubiquitination during Neural Development. [FBrf0213919] Distinct regulatory programs establish widespread sex-specific alternative splicing in Drosophila melanogaster. [FBrf0212988] Neural-specific elongation of 3' UTRs during Drosophila development. [FBrf0215804] A feedback loop between Wolbachia and the Drosophila gurken mRNP complex influences Wolbachia titer. [FBrf0217227] Drosophila histone locus bodies form by hierarchical recruitment of components. [FBrf0213662]
All reviews listed in FlyBase were published before 2011 
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