hrp48, p50, linha, RRM7, l(2)02647
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Gene model reviewed during 5.45
Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.
Hrb protein was purified by RNA affinity
chromatography. It was shown to recognize specific nucleotides in a
pseudo-5' splice site within the inhibitory element of the P-element third
intron. A correction to the published sequence is presented which predicts
a larger size for the Hrb27C protein.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hrb27C using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Hrb27C in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.
Identified as a 50kD protein that binds to a discrete translational derepressor element located at the 5' end of the osk transcript. Hrb27C protein also binds to the Bruno-response element (BRE) at the 3' end of the osk transcript.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Psi is a soma-specific alternative splicing factor that functions with general factors, including Hrb27C (a 50kD RNA-binding protein), to regulate germ line specific P-element expression. Hrb27C binds specifically to intron 3 (IVS3) 5' exon splice site.
A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs.
The distribution of the hnRNP protein Hrb27C on nascent transcripts from polytene chromosomes has been studied.
Protein has been isolated by its ability to bind single-stranded DNA.
Biochemical experiments support a mechanism for somatic inhibition of P-element intron 3 (IVS3) splicing in which the binding of a U1snRNP and multiprotein complexes to two exon pseudo 5' splice sites (F1 and F2) prevents U1snRNP binding to the accurate 5' splice site. A 50kD protein, Hrb27C contacts the F2 5' splice site.