• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\otk

General Information
Symbol	Dmel\otk	Species	D. melanogaster
Name	off-track	Annotation symbol	CG8967
Feature type	protein_coding_gene	FlyBase ID	FBgn0004839
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	Dtrk
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	48D6-48D7	Sequence location	2R:7,888,978..7,907,351 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene off-track is referred to in FlyBase by the symbol Dmel\otk (CG8967, FBgn0004839). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: cell adhesion molecule binding; receptor activity; Wnt-protein binding; protein kinase activity. There is experimental evidence that it is involved in the biological process: negative regulation of canonical Wnt receptor signaling pathway; axon guidance; cell adhesion. 20 alleles are reported. The phenotypes of these alleles are annotated with: wing margin; embryonic/first instar larval cuticle; intersegmental nerve; abdominal segmental nerve; axon; wing hair; denticle belt. It has one annotated transcript and one annotated polypeptide. Protein features are: Immunoglobulin I-set; Immunoglobulin subtype; Immunoglobulin subtype 2; Immunoglobulin-like domain; Immunoglobulin-like fold; Protein kinase, catalytic domain; Protein kinase-like domain; Serine-threonine/tyrosine-protein kinase catalytic domain; Tyrosine-protein kinase, active site; Tyrosine-protein kinase, catalytic domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1633753_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of otk. Gene sequence location is 2R:7888978..7907351.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 48D6-48D7   Limits computationally determined from genome sequence between P{lacW}l(2)k06612k06612 and P{lacW}jebk05644
Experimentally Determined Cytological Location
Cytogenetic map Notes References 48D-48D   (determined by in situ hybridisation)   (Pulido et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\otk for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) otk-RA FBtr0088028  NM_078981   4671   1033
Additional Transcript Data & Comments
Reported size (kB)	5.7 (northern blot) (Pulido et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein otk-PA   FBpp0087135   114.3   1033   6.88     NP_523705   AAF58596
Additional Polypeptide Data & Comments
Reported size (kDa)	1033 (aa); 160 (kD observed) (Pulido et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin subtype (IPR003599) Immunoglobulin-like domain (IPR007110) Tyrosine-protein kinase, active site (IPR008266) Protein kinase-like domain (IPR011009) Immunoglobulin-like fold (IPR013783) Tyrosine-protein kinase, catalytic domain (IPR020635) Serine-threonine/tyrosine-protein kinase catalytic domain (IPR001245) Immunoglobulin I-set (IPR013098)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58596   AI404212   AQ074029   BT015249 AAT94478   BT083425   CK661771   CO184108   CO192175   CO194574   CO305400   CO310470   CO344963   CQ847776   CZ482896   EC053991   EC054489   EC054972   EC062718   EC064675   EC072593   EC203414   EC213736   EC255182   EC255985   ES693787   ES694048   GH752389   GH839082   GH870999   GH879521   GH939725   X63453 CAA45053
UniProtKB/Swiss-Prot	Q6AWJ9
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435237 2R:7,896,113..7,898,348 evidence=Reporter construct (in vivo) (Jakobsen et al., 2007, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & pupal stage     (Pulido et al., 1992)
Additional Descriptive Data
	Two major peaks of otk expression are seen on northern blots. They occur in early-mid embryos and in early pupae. (Pulido et al., 1992)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage | late   peripheral nervous system | restricted   (Pulido et al., 1992) cell body   (Pulido et al., 1992) anterior commissure   (Pulido et al., 1992) posterior commissure   (Pulido et al., 1992) neuron   • axon (Pulido et al., 1992) embryonic stage 7   cephalic furrow   (Pulido et al., 1992) embryonic stage 7 -- 11   anterior midgut primordium   (Pulido et al., 1992) embryonic stage 11   procephalic segment   (Pulido et al., 1992) posterior midgut primordium   (Pulido et al., 1992) epidermis | restricted   (Pulido et al., 1992) neuroblast   (Pulido et al., 1992) embryonic stage 12   maxillary segment   (Pulido et al., 1992) epidermis | segmentally repeated   (Pulido et al., 1992) proctodeum | precursor   (Pulido et al., 1992) embryonic stage 12 -- 17   neuron   (Pulido et al., 1992) embryonic stage 13   ventral nerve cord   (Pulido et al., 1992) embryonic/larval midgut   (Pulido et al., 1992) embryonic/larval hindgut   (Pulido et al., 1992) visceral mesoderm   (Pulido et al., 1992)
Additional Descriptive Data
	otk protein expression is first detected at embryonic stage 7 in the anterior midgut primordium, cephalic furrow, and along the germ band. At stage 11, expression is observed in 15 stripes corresponding to parasegments 1-15, including external epidermal cells and internalized neuroblasts of the CNS. Within each parasegment, staining is in the posterior compartment. On the epidermis, staining comprises ventral and lateral regions but does not include the midline. Later, staining is observed in the anterior compartment. At the beginni g of germ band retraction, expression is observed in segmentally repeated patches of neuroepithelial cells and more strongly in underlying neuronal cells. It is also detected transiently in the maxillary bud. In stage 13, protein disappears from the epithelium and condenses in the ventral nerve cord. It is also observed in three discontinuous gut areas including the anterior midgut, posterior midgut and hindgut where it is restricted to the visceral mesoderm. At later stages, expression is barely detectable in mesodermal tissues but is strong in the ventral cord. Protein is present throughou the CNS on neuronal cell bodies and their axons. Staining is stronger in anterior commissures than posterior commissures. Protein is also detected on axons leaving the CNS through the segmental and intersegmental nerves, in neurons of a subset of sensory organs of the PNS, and on fasciculating axons growing toward muscle targets. (Pulido et al., 1992)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay (Pulido et al., 1992)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    592.425 Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (592.425) Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    592.425 Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    592.425 Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    592.425 Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  592.425 Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    592.425 Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    592.425 Larval Midgut    26.9 Larval Hindgut    54.5 Larval Malpighian Tubules    7.1 Larval Fat Body    14.3 Larval Salivary Gland    8.7 Larval Trachea    18.1 Larval Carcass    18.05 Adult Head    9.6 Adult Eye    3.175 Adult Brain    48 Adult Thoracic-Abdominal Ganglion    24.2 Adult Crop    19.7 Adult Midgut    14.7 Adult Hindgut    60.1 Adult Malpighian Tubules    21.7 Adult Fat Body    33.4 Adult Salivary Gland    15.3 Adult Heart    26.425 Adult VirginFemale Spermatheca    9.1 Adult InseminatedFemale Spermatheca    6.4 Adult Ovary    3.8 Adult Testis    8.7 Adult Male Accessory Gland    6.4 Adult Carcass    18.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    9.6   NA  Eye    3.175   NA  Brain    48   592.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    24.2   NA  Crop    19.7   26.9  Midgut    14.7   54.5  Hindgut    60.1   7.1  Malpighian Tubules    21.7   14.3  Fat Body    33.4   8.7  Salivary Gland    no informative data   NA  Heart    26.425   18.1  Trachea    NA   NA  VirginFemale Spermatheca    9.1   NA  InseminatedFemale Spermatheca    6.4   NA  Ovary    3.8   NA  Testis    8.7   NA  Male Accessory Gland    6.4   18.05  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    46 central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3  (46) central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    46 central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    46 central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    46 central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3  (46) central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    46 central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    46 central nervous system, pupae P8    20 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    13 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr    38 embryo 08-10hr    58 embryo 10-12hr    75 embryo 12-14hr    77 embryo 14-16hr    42 embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr    35 white prepupae 24hr    42 pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr  (38) embryo 08-10hr  (58) embryo 10-12hr  (75) embryo 12-14hr  (77) embryo 14-16hr  (42) embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr  (35) white prepupae 24hr  (42) pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr    38 embryo 08-10hr    58 embryo 10-12hr    75 embryo 12-14hr    77 embryo 14-16hr    42 embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr    35 white prepupae 24hr    42 pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr    38 embryo 08-10hr    58 embryo 10-12hr    75 embryo 12-14hr    77 embryo 14-16hr    42 embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr    35 white prepupae 24hr    42 pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr    38 embryo 08-10hr    58 embryo 10-12hr    75 embryo 12-14hr    77 embryo 14-16hr    42 embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr    35 white prepupae 24hr    42 pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr  (38) embryo 08-10hr  (58) embryo 10-12hr  (75) embryo 12-14hr  (77) embryo 14-16hr  (42) embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr  (35) white prepupae 24hr  (42) pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr    38 embryo 08-10hr    58 embryo 10-12hr    75 embryo 12-14hr    77 embryo 14-16hr    42 embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr    35 white prepupae 24hr    42 pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    16 embryo 06-08hr    38 embryo 08-10hr    58 embryo 10-12hr    75 embryo 12-14hr    77 embryo 14-16hr    42 embryo 16-18hr    19 embryo 18-20hr    13 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    17 white prepupae new    21 white prepupae 12hr    35 white prepupae 24hr    42 pupae 2d postWPP    20 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    10 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    5 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    8 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    4 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    25 ovary OSC    16 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004839 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8967-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die, with Scer\GAL4elav.PLu otkEP2017 viable otk2 otk8 viable, with Scer\GAL4pnr-MD237 otkGD66 otkGD2562 otkGD14399 Other Phenotypes Allele planar polarity defective, with Scer\GAL4ptc-559.1 otkScer\UAS.cWa visible, with Scer\GAL4ptc-559.1 otkScer\UAS.cWa wild-type otk2 otk8 Phenotype manifest in Allele abdominal segmental nerve otk1 otk3 abdominal segmental nerve, with Scer\GAL4elav.PLu otkEP2017 axon otk1 otk3 axon, with Scer\GAL4elav.PLu otkEP2017 denticle belt otk3 denticle belt | ectopic, with Scer\GAL4da.G32 otkScer\UAS.cWa embryonic/first instar larval cuticle, with Scer\GAL4da.G32 otkScer\UAS.cWa eye photoreceptor cell & axon & larva | somatic clone otk3 intersegmental nerve otk1 otk3 intersegmental nerve, with Scer\GAL4elav.PLu otkEP2017 lamina & glial cell & larva | somatic clone otk3 medulla | somatic clone otk3 photoreceptor cell R1 & axon & larva | somatic clone otk3 photoreceptor cell R1 & axon | somatic clone otk3 photoreceptor cell R2 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R2 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R2 & axon & larva | somatic clone otk3 photoreceptor cell R2 & axon | somatic clone otk3 photoreceptor cell R3 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R3 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R3 & axon & larva | somatic clone otk3 photoreceptor cell R3 & axon | somatic clone otk3 photoreceptor cell R4 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R4 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R4 & axon & larva | somatic clone otk3 photoreceptor cell R4 & axon | somatic clone otk3 photoreceptor cell R5 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R5 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R5 & axon & larva | somatic clone otk3 photoreceptor cell R5 & axon | somatic clone otk3 photoreceptor cell R6 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R6 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R6 & axon & larva | somatic clone otk3 photoreceptor cell R6 & axon | somatic clone otk3 wing hair, with Scer\GAL4ptc-559.1 otkScer\UAS.cWa wing margin, with Scer\GAL4ptc-559.1 otkScer\UAS.cWa
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of otk Class Mutagen Stocks Known lesion otkEP2017 P-element activity 2 Yes otkf00431 piggyBac transposase 1 -- otkMB06876 1 -- otk1 0 -- otk2 0 Yes otk3 loss of function allele 0 Yes otk8 0 Yes otkCPTI000252 piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of otk Class Mutagen Stocks Known lesion otkGD14399 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes otkGD2562 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes otkGD66 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes otkJF01796 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes otkKK103191 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes otkcPa in vitro construct 0 Yes otkExtra.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes otkHM05127 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes otkIntra.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes otkNIG.8967R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes otkScer\UAS.cWa in vitro construct - regulatory fusion 0 Yes otkScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD66} NA P{GD2562} NA P{GD14399} NA P{KK103191} NA P{NIG.8967R} NA P{TRiP.HM05127} NA P{TRiP.JF01796} NA P{UAS-otk.Extra} NA P{UAS-otk.HA} NA P{UAS-otk.Intra} NA P{UAS-otk.W} NA characterization construct P{UAS-fz2::otk.intra} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}otkEP2017 NA miscellaneous insertions PBac{602.P.SVS-1}otkCPTI000252 NA PBac{WH}otkf00431 NA insertion of enhancer trap binary system Mi{ET1}otkMB06876 No
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
cell adhesion molecule binding inferred from direct assay AND inferred from sequence or structural similarity (Pulido et al., 1992) protein kinase activity inferred from direct assay (Pulido et al., 1992) receptor activity inferred from direct assay AND inferred from sequence or structural similarity (Pulido et al., 1992) Wnt-protein binding inferred from direct assay (Peradziryi et al., 2011)
Biological Process
CV term Evidence References
axon guidance inferred from genetic interaction with Sema-1a AND inferred from genetic interaction with plexA AND inferred from mutant phenotype (Winberg et al., 2001) inferred from mutant phenotype (Cafferty et al., 2004) cell adhesion inferred from direct assay AND inferred from sequence or structural similarity (Pulido et al., 1992) negative regulation of canonical Wnt receptor signaling pathway inferred from mutant phenotype (Peradziryi et al., 2011)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Pulido et al., 1992)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719 (FlyBase Curators et al., 2004-) protein tyrosine kinase activity inferred from sequence or structural similarity with NCBI_gi:4506273 (FlyBase, 1992-) non-traceable author statement (Morrison et al., 2000) semaphorin receptor binding traceable author statement (Castellani and Rougon, 2002) transmembrane receptor protein tyrosine kinase activity inferred from sequence or structural similarity (Pulido et al., 1992)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR008266, InterPro:IPR020635 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions otk allele Gene References otk3 Sema-1a (Winberg et al., 2001) unspecified plexA (Winberg et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62067 DroID - A comprehensive database of gene and protein interactions. • FBgn0004839 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36283
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG67M2H8 Dipteran inclusive ortholog search EOG60K86V Insect inclusive ortholog search EOG6WSV5D Arthropod inclusive ortholog search EOG6RXWF2 Metazoa inclusive ortholog search EOG6CCGT0
Orthologs in Drosophila Species (EOG67M2H8)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\otk     Drosophila simulans   Dsim\GD25868 Y   Drosophila sechellia   Dsec\GM20393 Y   Drosophila erecta   Dere\GG22613 Y   Drosophila yakuba   Dyak\GE13482 Y   Drosophila ananassae   Dana\GF12649 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21443 Y   Drosophila persimilis   Dper\GL11545 Y   Drosophila willistoni   Dwil\GK21983 Y   Drosophila virilis   Dvir\GJ21665 Y   Drosophila mojavensis   Dmoj\GI18649 Y   Drosophila grimshawi   Dgri\GH21048 Y
Orthologs in non-Drosophila Dipterans (EOG60K86V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007876   Anopheles gambiae Malaria mosquito  Agam\AGAP011489   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006028
Orthologs in non-Dipteran Insects (EOG6WSV5D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45503 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014293 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16554 Y Atta cephalotes Leafcutter ant  Acep\ACEP26404 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO14415 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17264 Y Linepithema humile Argentine ant  Lhum\LH14768 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB13609 Y Solenopsis invicta Red fire ant  Sinv\SINV19577 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000999 Y Pediculus humanus Human body louse  Phum\PHUM363300 Y Tribolium castaneum Red flour beetle  Tcas\TC003353 Y
Orthologs in non-Insect Arthropods (EOG6RXWF2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\rps4 Y Daphnia pulex Water flea  Dpux\Tfb4 Y Ixodes scapularis Deer tick  Isca\ISCW015459 Y
Orthologs in non-Arthropod Metazoa (EOG6CCGT0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010698gn Y Danio rerio Zebrafish  Drer\zgc:112211 Y Xenopus tropicalis Western clawed frog  Xtro\ptk7 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000007469 Y Gallus gallus Domestic chicken  Ggal\PTK7 Y Mus musculus House mouse  Mmus\Ptk7 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000039976 Y Homo sapiens Human  Hsap\PTK7 Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\PTK7
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25868 Drosophila sechellia Dsec\GM20393 Drosophila erecta Dere\GG22613 Drosophila yakuba Dyak\GE13482 Drosophila ananassae Dana\GF12649 Drosophila pseudoobscura pseudoobscura Dpse\GA21443 Drosophila persimilis Dper\GL11545 Drosophila willistoni Dwil\GK21983 Drosophila virilis Dvir\GJ21665 Drosophila mojavensis Dmoj\GI18649 Drosophila grimshawi Dgri\GH21048
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	25334 w1118; Mi{ET1}otkMB06876 17231 w1118; P{EP}otkEP2017/CyO 25790 y1 v1; P{TRiP.JF01796}attP2
Harvard	- PBac{WH}otkf00431
Kyoto	114461 yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D P{EP}otkEP2017 /CyO y+
VDRC	v104688 P{KK103191}VIE-260B v878 w1118; P{GD66}v878 v879 w1118; P{GD66}v879 v42565 w1118; P{GD2562}v42565 v42566 w1118; P{GD2562}v42566 v30833 w1118; P{GD14399}v30833 v30834 w1118; P{GD14399}v30834
Genomic Clones ( 2 )
	BACR04E21 BACR14L19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 25 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP17455
Other clones	RT02641
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH23842 LP21473
Other clones	17207 57559 99149 103560 145876 214411.26 214537.31 EC27129 EC33294 EC38829 EC053991 EC054489 EC054972 EK221760 EK248455 EP21032 RP003142534 RP003278414 RP003404352 RP003520603 RP003613493
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004839 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36283
Antibody Information
	polyclonal (Pulido et al., 1992, Peradziryi et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Phylogenetic analysis of the PTK family. (Suga et al., 1997) The otk gene product promotes cell adhesion in a homophilic calcium- dependent manner and this adhesion process specifically activates its kinase behaviour. (Pulido, 1993) Identification: By sequence similarity to human trk (receptor tyrosine kinase) cDNA probes. (Pulido et al., 1992) otk has been identified, sequence and its expression pattern characterised. (Pulido et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078981 RefSeq (Proteins) • NP_523705 DDBJ / EMBL / GenBank • AAF58596 AAT94478 AQ074029 BT015249 CAA45053 CQ847776 CZ482896 X63453 Entrez Gene - A searchable database of RefSeq genes. • 36283 UniProtKB/Swiss-Prot • Q6AWJ9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin subtype (IPR003599) Immunoglobulin-like domain (IPR007110) Tyrosine-protein kinase, active site (IPR008266) Protein kinase-like domain (IPR011009) Immunoglobulin-like fold (IPR013783) Tyrosine-protein kinase, catalytic domain (IPR020635) Serine-threonine/tyrosine-protein kinase catalytic domain (IPR001245) Immunoglobulin I-set (IPR013098)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62067 DroID - A comprehensive database of gene and protein interactions. • FBgn0004839 DRSC - Results from RNAi screens. • FBgn0004839 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004839 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8967-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004839 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36283 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/offtrack1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36283 modMine - Data generated by the modENCODE project. • FBgn0004839
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	anon-WO2004063362.81   CG8967 (Morrison et al., 2000, Perkins et al., 1.15.2009, Keleman et al., 2009.8.5, Perkins et al., 2009) CT25769 (Hynes and Zhao, 2000) Dtkr (Söderberg et al., 2011) Dtrk (Hidalgo, 2002, Jaaro et al., 2001, Goodman, 1999.8.19, Suga et al., 1997, Pulido, 1993, Pulido et al., 1991, Gomez-Skarmeta et al., 1993, Steller and Grether, 1994, Bieber, 1994, Perrimon, 1994, Pulido et al., 1992, Barbacid et al., 1991) dTrk (Castellani and Rougon, 2002) DTrk (Bai and Montell, 1999, Bai and Montell, 1998) IGGtyk   OFT/TRK (Morrison et al., 2000) OTK (Terman and Kolodkin, 2004) Otk (Cafferty et al., 2004, Minidorff et al., 2007, Jeong et al., 2012) otk (Bakal et al., 2008, Zeitlinger et al., 2007, Peradziryi et al., 2011, Japanese National Institute of Genetics, 2012.5.21) Tk48D   Trk48D
Name Synonym	Off-track (Terman and Kolodkin, 2004) off-track   Tyrosine kinase at 48D
Secondary FlyBase IDs
	FBgn0082205
References ( 59 )
Generate a list of	All references relating to this gene ( 59 )
List References by type	Research paper  ( 12 ) Supplementary material  ( 6 ) Review  ( 14 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 7 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Patent  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Jeong et al., 2012, Neuron 76(4): 721--734 The Control of Semaphorin-1a-Mediated Reverse Signaling by Opposing Pebble and RhoGAPp190 Functions in Drosophila. [FBrf0219982] Peradziryi et al., 2011, EMBO J. 30(18): 3729--3740 PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. [FBrf0215241] Söderberg et al., 2011, PLoS ONE 6(5): e19866 Insulin Production and Signaling in Renal Tubules of Drosophila Is under Control of Tachykinin-Related Peptide and Regulates Stress Resistance. [FBrf0213657] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...