FlyBase Gene Report: Dmel\RpS2

General Information

Symbol

Dmel\RpS2

Species

D. melanogaster

Name

Ribosomal protein S2

Annotation Symbol

CG5920

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004867

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Ribosomal protein S2 (RpS2) encodes a highly conserved component of the 40S ribolomal comlex. RpS2 product controls the assembly and nuclear export of the pre-ribosome and appears to have a specific function during oogenesis. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

sop, string of pearls, l(2)03848, l(2)k04603, S2

Key Links

Genomic Location

Cytogenetic map

30E1-30E1

Sequence location

2L:9,896,265..9,897,552 [-]

Recombination map

2-37

RefSeq locus

NT_033779 REGION:9896265..9897552

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

inferred from direct assay

(Anger et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables RNA binding

inferred from electronic annotation with InterPro:IPR000851, InterPro:IPR013810

(InterPro Project Members, 2004-)

enables structural constituent of ribosome

traceable author statement

(Marygold et al., 2007)

inferred from electronic annotation with InterPro:IPR000851, InterPro:IPR005324, InterPro:IPR005711, InterPro:IPR013810

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000346706

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cytoplasmic translation

inferred by curator from GO:0022626

(Anger et al., 2013, Alonso and Santaren, 2006)

traceable author statement

(Marygold et al., 2007)

involved_in translation

inferred from biological aspect of ancestor with PANTHER:PTN000346706

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR000851, InterPro:IPR005324, InterPro:IPR005711, InterPro:IPR013810

(InterPro Project Members, 2004-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Rugjee et al., 2013)

located_in cytosolic ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

inferred from direct assay

(Anger et al., 2013)

colocalizes_with nuclear chromosome

inferred from direct assay

(Rugjee et al., 2013)

located_in nucleolus

inferred from direct assay

(Rugjee et al., 2013)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of cytosolic small ribosomal subunit

traceable author statement

(Marygold et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000346706

(GO Reference Genome Project, 2011-)

located_in ribosome

inferred from electronic annotation with InterPro:IPR000851, InterPro:IPR005324, InterPro:IPR013810

(InterPro Project Members, 2004-)

part_of small ribosomal subunit

inferred from electronic annotation with InterPro:IPR005711

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS

Protein Family (UniProt)

Belongs to the universal ribosomal protein uS5 family. (P31009)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)

Protein Function (UniProtKB)

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Plays a role in the assembly and function of the 40S ribosomal subunit. Mutations in this protein affects the control of translational fidelity. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity). Has a specific developmental role during oogenesis (PubMed:7982558).

(UniProt, P31009)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RpS2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P31009)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpS2-RA

FBtr0079905

NM_057526

916

267

RpS2-RB

FBtr0330682

NM_001273374

1177

267

RpS2-RC

FBtr0345436

NM_001298856

947

267

Additional Transcript Data and Comments

Reported size (kB)

0.9 (northern blot)

(Cramton and Laski, 1994, Barrio et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RpS2-PA

FBpp0079500

28.9

267

10.78

FBpp0303531

28.9

267

10.78

FBpp0311561

28.9

267

10.78

P31009

NP_001285785

AHN54299

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

267 aa isoforms: RpS2-PA, RpS2-PB, RpS2-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

267 (aa)

(Cramton and Laski, 1994, Barrio et al., 1993)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.83

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

ovary

(Barrio et al., 1993)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Cramton and Laski, 1994)

adult stage | female

(Cramton and Laski, 1994)

adult stage & oogenesis

nurse cell

(Cramton and Laski, 1994)

oogenesis

organism | ubiquitous

(Cramton and Laski, 1994)

Additional Descriptive Data

sop transcripts are expressed at approximately the same level at all stages of embryonic development as well as in adult ovaries and whole adult flies. In situ hybridization reveals ubiquitous expression in embryos and throughout oogenesis with expression heaviest in nurse cells.

(Cramton and Laski, 1994)

sop transcripts are preferentially transcribed in exponentially growing cells and are particularly abundant in ovaries.

(Barrio et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Rugjee et al., 2013)

located_in cytosolic ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

inferred from direct assay

(Anger et al., 2013)

colocalizes_with nuclear chromosome

inferred from direct assay

(Rugjee et al., 2013)

located_in nucleolus

inferred from direct assay

(Rugjee et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RpS2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpS2

View exonic expression by tissue for Dmel\RpS2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004867

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34309/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004867

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004867

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004867

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5920

Flygut - An atlas of the Drosophila adult midgut

FBgn0004867

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpS2

Transgenic constructs containing regulatory region of RpS2

Aberrations (Deficiencies and Duplications) ( 11 )

Inferred from experimentation ( 11 )

Gene not disrupted in

Df(2L)30A-C

(Watson et al., 2010, BDGP Project Members, 1994-1999)

Df(2L)s1402

(Watson et al., 2010, BDGP Project Members, 1994-1999)

Inferred from location ( 14 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

RpS2^CB02294

lethal, with Scer\GAL4^hs.2sev

RpS2^GS2141

lethal, with Scer\GAL4^pnr-MD237

RpS2^GD9879

lethal | recessive

lethal - all die before end of larval stage | recessive

lethal - all die during first instar larval stage

RpS2^PRW1

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

RpS2^GD9879

partially lethal, with Scer\GAL4^tin.CΔ4

RpS2^GD9879

partially lethal - majority die, with Scer\GAL4^29BD

RpS2^GS2141

partially lethal - majority die, with Scer\GAL4^c355

RpS2^GS2141

viable

RpS2^P

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpS2^GD9879

Sterility

Allele

female sterile

RpS2^P

female sterile | recessive

RpS2^P

Other Phenotypes

Allele

abnormal cell cycle

RpS2^P

abnormal cell cycle (with RpS2^P)

RpS2^PRW1

abnormal cell cycle (with RpS2^PRW1)

RpS2^P

some die during larval stage | recessive

visible, with Scer\GAL4^29BD

RpS2^GS2141

visible, with Scer\GAL4^dpp.blk1

RpS2^GS2141

visible | recessive

RpS2^P

Phenotype manifest in

Allele

anterior crossvein, with Scer\GAL4^dpp.blk1

RpS2^GS2141

egg chamber

RpS2^P

egg chamber (with RpS2^P)

RpS2^PRW1

egg chamber (with RpS2^PRW1)

RpS2^P

female germline stem cell

RpS2^P

female germline stem cell (with RpS2^P)

RpS2^PRW1

female germline stem cell (with RpS2^PRW1)

RpS2^P

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

RpS2^GD9879

macrochaeta

oocyte | oogenesis stage S5

RpS2^P

ovary

ovary | adult stage

RpS2^P

ovary | adult stage (with RpS2^P)

RpS2^PRW1

ovary | adult stage (with RpS2^PRW1)

RpS2^P

sarcomere, with Scer\GAL4^Mef2.PR

RpS2^GD9879

spectrosome

RpS2^P

spectrosome (with RpS2^P)

RpS2^PRW1

spectrosome (with RpS2^PRW1)

RpS2^P

wing, with Scer\GAL4^29BD

RpS2^GS2141

Z disc, with Scer\GAL4^Mef2.PR

RpS2^GD9879

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\RPS2

14 of 14

Yes

Hsap\MRPS5

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Rps2

14 of 14

Yes

Rnor\Rps2-ps6

10 of 14

Yes

Rnor\LOC100125366

2 of 14

Yes

Rnor\Mrps5

2 of 14

Rnor\RGD1559516

2 of 14

Yes

Rnor\RGD1562399

2 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Rps2

12 of 14

Yes

Mmus\Mrps5

2 of 14

Xenopus tropicalis (Western clawed frog) (2)

Xtro\rps2

8 of 13

Yes

Xtro\igsf10

1 of 13

Danio rerio (Zebrafish) (2)

Drer\rps2

14 of 14

Yes

Drer\mrps5

2 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\rps-2

14 of 14

Yes

Cele\mrps-5

2 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP003768

11 of 12

Yes

Agam\AgaP_AGAP004091

2 of 12

Arabidopsis thaliana (thale-cress) (8)

Atha\AT1G58684

13 of 13

Yes

Atha\AT1G58983

13 of 13

Yes

Atha\AT1G59359

13 of 13

Yes

Atha\AT2G41840

13 of 13

Yes

Atha\AT3G57490

13 of 13

Yes

Atha\XW6

13 of 13

Yes

Atha\EMB3113

3 of 13

Atha\AT1G64880

2 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\RPS2

13 of 13

Yes

Scer\MRPS5

2 of 13

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rps2

12 of 12

Yes

Spom\mrps5

2 of 12

Escherichia coli (enterobacterium) (1)

Ecol\rpsE

5 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:RpS2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

mRpS5

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPS2; ribosomal protein S2

14 of 14

603624

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpS2 - Dsor1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpS2 - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - Ras85D

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpS2 - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL37-1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS15Aa

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - Zfrp8

two hybrid, anti tag coimmunoprecipitation, western blot

(Minakhina et al., 2016)

RpS2 - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS2 - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpS2 - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS2 - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

RpS2

enhanceable

Zfrp8

(Minakhina et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nsf2

suppressible

RpS2

(Laviolette et al., 2005)

Zfrp8

enhanceable

RpS2

(Minakhina et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60403

DroID - A comprehensive database of gene and protein interactions.

FBgn0004867

MIST (protein-protein) - An integrated Molecular Interaction Database

34309

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-37

Cytogenetic map

30E1-30E1

Sequence location

2L:9,896,265..9,897,552 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

30E1-30E1

Limits computationally determined from genome sequence between P{lacW}l(2)k06416^k06416&P{lacW}sop^k04603 and P{EP}CG5899^EP701

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

30E1-30E2

(determined by in situ hybridisation)

(Spradling et al., 1999)

30E1-30E2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-37

(FlyBase, 2016)

2-32.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (799)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD24077

Other clones

DPiM_LD24077

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004867

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004867

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Nishiyama et al., 2007)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Not a Minute gene.

(Marygold et al., 2007)

Deletions removing sop but no other cytoplasmic ribosomal protein-encoding genes do not show Minute phenotypes.

(Marygold et al., 2007)

Molecularly-defined mutations in sop do not result in Minute phenotypes.

(Marygold et al., 2007)

sop encodes the Drosophila homologue of eukaryotic ribosomal protein S2. sop may have a specific role during oogenesis in addition to its general role in protein synthesis.

(Cramton and Laski, 1994)

There are several copies of ribosomal protein S2 encoding genes in the genome.

(Barrio et al., 1993)

A cDNA encoding a ribosomal protein S2 characterized.

(Barrio et al., 1993)

Defined by insertion of an enhancer trap element.

(Timmons et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: sop l(2)03848

(Spradling et al., 1999)

Source for merge of: sop l(2)k04603

(Beaton, 1999.12.12)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: RpS2 sop

(Barrio et al., 1993)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

CG5920

(National Institute of Genetics Fly Stocks, 2022-, Kolkhof et al., 2017, Rosa-Ferreira et al., 2015, Fukuoh et al., 2014, Schertel et al., 2013, Cammarato et al., 2011, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Marygold et al., 2007, Quinones-Coello, 2007, Cermelli et al., 2006, Laviolette et al., 2005)

Dm01335

(Joshi and Nguyen, 1995)

Rp S2

(Alonso and Santaren, 2006)

(Molnar et al., 2003, Barrio et al., 1993)

(Anger et al., 2013)

anon-EST:Posey202

dS2

(Barrio, 1992.11.5)

l(2)03848

(Rugjee et al., 2013, BDGP Project Members, 1994-1999, Timmons et al., 1993)

l(2)k04603

(BDGP Project Members, 1994-1999)

rpS2

(Nishiyama et al., 2007)

rps2

(Blatt et al., 2020)

sop

(Lim et al., 2016, Jagut et al., 2013, Rugjee et al., 2013, Schertel et al., 2013, Fischer et al., 2012, Baxley et al., 2011, Friedman et al., 2011, Müller et al., 2010, Parry et al., 2010, Watson et al., 2010, Benoit et al., 2008, Buszczak et al., 2007, Marygold et al., 2007, Quinones-Coello, 2007, Quinones-Coello et al., 2007, Christensen and Cook, 2006.12.5, Laviolette et al., 2005, Wang et al., 2004)

Name Synonyms

40S ribosomal protein S2

(Costa et al., 2013)

Ribosomal protein S2

(Ashburner and Marygold, 2004.7.2)

Ribosome RS2

(Saurin et al., 2001)

S2 ribosomal protein

(Barrio et al., 1993)

String of pearls

(Costa et al., 2013)

ribosomal protein S2

(Lim et al., 2016, Barrio et al., 1993)

string of pearls

(Cammarato et al., 2011, Carpenter et al., 2009, Marygold et al., 2007, Quinones-Coello et al., 2007, Cermelli et al., 2006)

Secondary FlyBase IDs

FBgn0010266
FBgn0013522
FBgn0022202
FBgn0025306

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 74 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34309

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P31009

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P31009

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PB84

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P31009

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004867

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34309/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004867

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004867

FlyMine - An integrated database for Drosophila genomics

FBgn0004867

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5920

MARRVEL_MODEL - MARRVEL (model organism gene)

34309

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

60403

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004867

DroID - A comprehensive database of gene and protein interactions.

FBgn0004867

DRSC - Results frm RNAi screens

FBgn0004867

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004867

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004867

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5920

Flygut - An atlas of the Drosophila adult midgut

FBgn0004867

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004867

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007364

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

34309

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (124)

The following external sites may use different assemblies or annotations than FlyBase.

-0.83

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