a novel protein involved in stem cell renewal and asymmetric cell division - piRNA-guided slicing of transposon transcripts enforces their transcriptional silencing via specifying the nuclear piRNA repertoire - maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins Piwi modulates chromatin accessibility by regulating multiple factors including Histone H1 to repress transposons
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.50
843 (aa); 97.2 (kD predicted)
In the ovaries, part of a complex composed of at least Panx, nxf2, piwi and Nxt1 (PubMed:26472911, PubMed:26494711, PubMed:31368590, PubMed:31384064). The complex is knowns as Panx-induced co-transcriptional silencing (PICTS) complex, Panx-nxf2-dependent TAP/p15 silencing (Pandas complex), SFiNX (silencing factor interacting nuclear export variant) or piwi-Panx-nxf2-p15 (PPNP) complex (PubMed:26472911, PubMed:26494711, PubMed:31368590, PubMed:31384064). Interacts with vas; this interaction is RNA-independent (PubMed:16949822). Interacts with Dcr-1 and Fmr1; these interactions occur in polar granules (PubMed:16949822). Interacts (via N-terminal region) with CBX5 (via chromoshadow domain) (PubMed:17875665). Forms a complex with Hsp83 and Hop; probably Hop mediates the interaction between piwi and Hsp83 (PubMed:21186352). Forms a complex with Yb body components armi and fs(1)Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi (PubMed:20966047). Interaction of Piwi and fs(1)Yb is likely to occur via armi (PubMed:20966047). Interacts (via the N-terminal region when unmethylated or symmetrically methylated at Arg-10) with papi (via Tudor domain) (PubMed:21447556, PubMed:29531043). Interacts with vret (PubMed:21831924). Interacts with Panx (PubMed:26472911, PubMed:26494711). Interacts with arx (PubMed:23913921, PubMed:23913922). Interacts with Tudor-SN (PubMed:26808625). Interacts with Nup358 (via N-terminus) (PubMed:29735528). Associates with the nuclear pore complex via interaction with Elys (PubMed:28472469). Interacts with thoc5; the interaction might be partly RNA-mediated (PubMed:28472469). Interacts with xmas-2 (PubMed:28472469).
Symmetrically dimethylated, most likely by csul (PubMed:19377467, PubMed:29531043). Methylation at Arg-10 enhances binding to papi whereas methylation at Arg-7, Arg-9 or Arg-11 reduces binding affinity to papi (PubMed:29531043).
Phosphorylated on serine and tyrosine residues in an Hsp83-dependent manner.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\piwi using the Feature Mapper tool.
piwi transcript is detected in germline and soma cells during oogenesis. In the germarium, expression is detected in the terminal filament cells and the epithelial sheath cells. In germ cells, expression is detected as early as germarium region 2, continues during stages S1-S6 of oogenesis, is much diminished but still detectable between S7-S9, and is again at high levels at stage S10. In early embryos, expression is uniform.
GBrowse - Visual display of RNA-Seq signalsView Dmel\piwi in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: piwi CG6122
piwi-RISC (RNA-induced silencing complex) mediates silencing of transposable elements at the transcriptional level, and this is accompanied by local heterochromatin formation.
piwi is required for maintenance of long-distance chromosomal interactions between endogenous PcG target loci.
piwi protein is localised to the nucleus and is specifically associated with rasiRNAs (repeat-associated small RNAs) derived from various repetitive elements in the genome such as retrotransposons and heterochromatic regions in vivo and shows shows target RNA cleavage ("Slicer") activity in the presence of a single-stranded guide siRNA in vitro.
Expression is enriched in embryonic gonads.
The piwi product binds directly to repeat-associated small interfering RNAs (rasiRNAs).
piwi is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs).
piwi is required for the silencing of retrotransposons in the male germline.
piwi has a cell autonomous function in promoting stem cell division in the germline.
piwi mediates a somatic signalling mechanism required for the asymmetric division of germ-line stem cells to produce and maintain a daughter germ-line stem cell but is not essential for the further differentiation of the committed daughter cell.
Mutant analysis demonstrates the piwi function is required both to maintain germline stem cells and subsequently for the division and differentiation of the stem cell progeny in both sexes. Mutations abolish the proliferation ability of both female and male germline stem cells.