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Gene Dmel\piwi

General Information
Symbol	Dmel\piwi	Species	D. melanogaster
Name	piwi	Annotation symbol	CG6122
Feature type	protein_coding_gene	FlyBase ID	FBgn0004872
Gene Model Status	Current	Stock availability	8 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	32C1-32C1	Sequence location	2L:10,982,205..10,987,416 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene piwi is referred to in FlyBase by the symbol Dmel\piwi (CG6122, FBgn0004872). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; RNA binding. There is experimental evidence that it is involved in the biological process: positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; posttranscriptional gene silencing; male germ-line stem cell division; negative regulation of transposition, DNA-mediated; pole cell formation; stem cell division; female germ-line stem cell division; chromatin silencing. 28 alleles are reported. The phenotypes of these alleles are annotated with: ovariole; egg; ovary; male germline stem cell; spectrosome; testis; nurse cell spectrosome; female germline stem cell; egg chamber; germarium. It has one annotated transcript and one annotated polypeptide. Protein features are: Argonaute/Dicer protein, PAZ; Ribonuclease H-like; Stem cell self-renewal protein Piwi. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1641305_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of piwi. Gene sequence location is 2L:10982205..10987416.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation. Saito et al., 2010, Genes Dev. 24(22): 2493--2498
FB2012_01	Sequence features HFA03592 BKN24486 Controlled Vocabulary Terms positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Yb body Alleles piwi[Scer\UAS.P\T.T:Ivir\HA1] References Handler et al., 2011, EMBO J. 30(19): 3977--3993 Rouget et al., 2010, Nature 467(7319): 1128--1132 Thomsen et al., 2010, Genome Biol. 11(9): R93 Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711 Parrott et al., 2011, PLoS ONE 6(9): e25087 Stefanov et al., 2010, Mol. Genet. Genomics 284(5): 373--381 Zamparini et al., 2011, Development 138(18): 4039--4050 Liu et al., 2010, Genes Dev. 24(17): 1876--1881
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 32C1-32C1   Limits computationally determined from genome sequence between P{lacW}l(2)k05812k05812&P{lacW}l(2)k09104k09104 and P{EP}ppoEP2478&P{lacW}l(2)k15817k15817
Experimentally Determined Cytological Location
Cytogenetic map Notes References 32B-32C   (determined by in situ hybridisation)   (Spradling et al., 1999) 32C-32C   (determined by in situ hybridisation)   (Lin and Spradling, 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\piwi for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) piwi-RA FBtr0080166  NM_057527   3069   843
Additional Transcript Data & Comments
Reported size (kB)	3.1 (northern blot) (Cox et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein piwi-PA   FBpp0079755   97.2   843   10.00     NP_476875   AAF53043
Additional Polypeptide Data & Comments
Reported size (kDa)	843 (aa); 97.2 (kD predicted) (Cox et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Argonaute/Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165) Ribonuclease H-like (IPR012337)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202789   AA802575   AA978826   AE014134 AAF53043   AC092226   AF104354 AAD08704   AF104355 AAD08705   AI061964   AQ025014   AW941950   BG637778   BG637781   BI163833   BI168498   BI214891   BI241150   BI354756   BI372643   BP542472   BP542762   BP543149   BP543150   BP543973   BP543974   BP553307   BT011097   BT011138   BT071791   CO177475   CO179730   CO186326   CO324805   CZ478664   EC057157   EC063398   EC207389   EC241555   EC255169   EV580069   GH753605   GH771553   GH773386   GH774539   GH782767   GH783947   GH784070   GH786807   GH789252   GH798223   GH802358   GH808269   GH809208   GH814120   GH814491   GH815147   GH816164   GH822098   GH826030   GH830182   GH835937   GH838619   GH841491   GH841794   GH849191   GH853553   GH861045   GH865154   GH868393   GH869010   GH869015   GH869374   GH872701   GH872834   GH882114   GH883780   GH884871   GH895879   GH898664   GH899792   GH900445   GH903416   GH905448   GH906054   GH909491   GH916595   GH922257   GH922543   GH923727   GH923815   GH926241   GH933216   GH937264   GH939474   GH940007
UniProtKB/Swiss-Prot	Q9VKM1
UniProtKB/TrEMBL	Q95SC7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Cox et al., 1998) adult stage & oogenesis   germarium region 2b   (Cox et al., 1998) germarium region 2a   (Cox et al., 1998) epithelial sheath   (Cox et al., 1998) ovary   (Cox et al., 1998) terminal filament basal cell   (Cox et al., 1998)
Additional Descriptive Data
	piwi transcript is detected in germline and soma cells during oogenesis. In the germarium, expression is detected in the terminal filament cells and the epithelial sheath cells. In germ cells, expression is detected as early as germarium region 2, continues during stages S1-S6 of oogenesis, is much diminished but still detectable between S7-S9, and is again at high levels at stage S10. In early embryos, expression is uniform. (Cox et al., 1998)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0004872    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0004872 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0004872 expression level Developmental Stage   Expression Level embryo 00-02hr    5782 embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day    2799 adult female 05day    5759 adult female 30day    5238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5782) embryo 02-04hr  (708) embryo 04-06hr  (386) embryo 06-08hr  (134) embryo 08-10hr  (168) embryo 10-12hr    88 embryo 12-14hr  (169) embryo 14-16hr  (186) embryo 16-18hr  (204) embryo 18-20hr  (154) embryo 20-22hr  (341) embryo 22-24hr  (387) larva L1  (179) larva L2  (130) larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr  (122) white prepupae 24hr  (152) pupae 2d postWPP  (152) pupae 3d postWPP  (212) pupae 4d postWPP  (496) adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day  (2799) adult female 05day  (5759) adult female 30day  (5238) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5782) embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day  (2799) adult female 05day  (5759) adult female 30day  (5238) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5782 embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day    2799 adult female 05day    5759 adult female 30day    5238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5782 embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day    2799 adult female 05day    5759 adult female 30day    5238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0004872 expression level Developmental Stage   Expression Level embryo 00-02hr    5782 embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day    2799 adult female 05day    5759 adult female 30day    5238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5782) embryo 02-04hr  (708) embryo 04-06hr  (386) embryo 06-08hr  134 embryo 08-10hr  (168) embryo 10-12hr    88 embryo 12-14hr  (169) embryo 14-16hr  (186) embryo 16-18hr  (204) embryo 18-20hr  (154) embryo 20-22hr  (341) embryo 22-24hr  (387) larva L1  (179) larva L2  130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr  122 white prepupae 24hr  (152) pupae 2d postWPP  (152) pupae 3d postWPP  (212) pupae 4d postWPP  (496) adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day  (2799) adult female 05day  (5759) adult female 30day  (5238) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5782) embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day  (2799) adult female 05day  (5759) adult female 30day  (5238) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5782 embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day    2799 adult female 05day    5759 adult female 30day    5238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5782 embryo 02-04hr    708 embryo 04-06hr    386 embryo 06-08hr    134 embryo 08-10hr    168 embryo 10-12hr    88 embryo 12-14hr    169 embryo 14-16hr    186 embryo 16-18hr    204 embryo 18-20hr    154 embryo 20-22hr    341 embryo 22-24hr    387 larva L1    179 larva L2    130 larva L3 12hr old    40 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    84 white prepupae new    73 white prepupae 12hr    122 white prepupae 24hr    152 pupae 2d postWPP    152 pupae 3d postWPP    212 pupae 4d postWPP    496 adult male 01day    71 adult male 05day    77 adult male 30day    87 adult female 01day    2799 adult female 05day    5759 adult female 30day    5238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0004872    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0004872 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0004872 expression level Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    583.7 Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary  (583.7) Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    583.7 Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    583.7 Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0004872 expression level Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    583.7 Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary  583.7 Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    583.7 Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    11.875 Larval Midgut    9 Larval Hindgut    9.3 Larval Malpighian Tubules    9.3 Larval Fat Body    49.8 Larval Salivary Gland    6.9 Larval Trachea    5.35 Larval Carcass    8.45 Adult Head    6.3 Adult Eye    5.375 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    9.2 Adult Hindgut no informative data Adult Malpighian Tubules    8.4 Adult Fat Body no informative data Adult Salivary Gland    8.8 Adult Heart    6.65 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    7.2 Adult Ovary    583.7 Adult Testis    18.2 Adult Male Accessory Gland    7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut     Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    6.3   NA  Eye    5.375   NA  Brain    no informative data   11.875  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   9  Midgut    9.2   9.3  Hindgut    no informative data   9.3  Malpighian Tubules    8.4   49.8  Fat Body    no informative data   6.9  Salivary Gland    8.8   NA  Heart    6.65   5.35  Trachea    NA   NA  VirginFemale Spermatheca    6.5   NA  InseminatedFemale Spermatheca    7.2   NA  Ovary    583.7   NA  Testis    18.2   NA  Male Accessory Gland    7   8.45  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_26
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004872 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4636-RA;CG6122-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for piwi Stages(s) 1-3 Stages(s) 4-6 Stages(s) 9-10 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 piwiGD11827 lethal | non-rescuable maternal effect piwi1 Sterility Allele female sterile piwi1 piwi2 piwi06843 female sterile, with Scer\GAL4nos.PG, Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4otu.T:Hsim\VP16 piwidsRNA.shRNA.Scer\UAS.P\T female sterile | recessive piwi1 piwi06843 male fertile piwi5'T:Hsap\MYC male sterile piwi1 male sterile | recessive piwi1 Other Phenotypes Allele enhancer of variegation, with P{wA}4-4 piwi2 increased cell death (with piwi1) piwi2 increased cell death (with piwi2) piwi1 mitotic cell cycle defective | germline clone piwi1 piwi2 piwi06843 suppressor of variegation piwi1 piwi2 suppressor of variegation, with P{T1(-FRT)}T190-177 piwi1 suppressor of variegation, with P{T1}T190-177 piwi1 wild-type piwi5'T:Hsap\MYC piwi+t6.8 Phenotype manifest in Allele cephalopharyngeal skeleton | embryonic stage | maternal effect piwi1 cystoblast | non-rescuable maternal effect piwi1 egg piwi1 egg chamber piwi2 embryo | germline clone piwi06843 piwi2 embryo | germline clone | non-rescuable maternal effect piwi1 embryonic/larval cuticle | embryonic stage | germline clone | maternal effect piwi1 embryonic head | germline clone piwi06843 piwi2 embryonic head | germline clone | non-rescuable maternal effect piwi1 female germline stem cell piwi06843 piwi1 piwi12 piwi2 female germline stem cell | cell autonomous | somatic clone piwi1 piwi2 female germline stem cell | ectopic, with Scer\GAL4hs.PB piwiEP1024 female germline stem cell | non-rescuable maternal effect piwi1 germarium piwi06843 piwi1 piwi2 piwiunspecified germarium, with Scer\GAL4hs.PB piwiEP1024 germarium | non-rescuable maternal effect piwi1 germline cell | female piwiunspecified germline cell | female, with Scer\GAL4hs.PB piwiEP1024 germline cyst | non-rescuable maternal effect piwi1 male germline stem cell piwi1 nurse cell spectrosome, with Scer\GAL4hs.PB piwiEP1024 ovariole piwi06843 piwi1 piwi2 ovariole (with piwi1), with Scer\GAL4[-]: piwiEP1024 ovariole (with piwiEP1024), with Scer\GAL4[-]: piwi1 ovariole | non-rescuable maternal effect piwi1 ovary (with piwi1) piwi2 ovary (with piwi1), with Scer\GAL4[-]: piwiEP1024 ovary (with piwi2) piwi1 ovary (with piwiEP1024), with Scer\GAL4[-]: piwi1 pole cell | germline clone | maternal effect piwi1 pole cell | maternal effect | supernumerary piwi5'T:Hsap\MYC spectrosome, with Scer\GAL4hs.PB piwiEP1024 spectrosome | ectopic, with Scer\GAL4hs.PB piwiEP1024 syncytial blastoderm embryo | germline clone piwi06843 piwi2 syncytial blastoderm embryo | germline clone | non-rescuable maternal effect piwi1 testis (with piwi1), with Scer\GAL4[-]: piwiEP1024 testis (with piwiEP1024), with Scer\GAL4[-]: piwi1
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of piwi Class Mutagen Stocks Known lesion piwi06843 P-element activity 2 Yes piwie00824 piggyBac transposase 1 -- piwi12 P-element activity 0 Yes piwi1 P-element activity 0 Yes piwi2 P-element activity 0 Yes piwi4 P-element activity 0 Yes piwiCPTI003588 piggyBac transposase 0 Yes piwiEP1024 wild-type allele, loss of function allele P-element activity 0 Yes piwif07053a 0 -- piwiunspecified 0 -- piwiΔ37 0 --
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of piwi Class Mutagen Stocks Known lesion piwiGD11827 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes piwiHMS00185 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes piwiHMS00606 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes piwiJF01394 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes piwiKK105350 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes piwi+t6.8 in vitro construct - genomic fragment 0 Yes piwi3'T:Hsap\MYC in vitro construct - coding region fusion 0 Yes piwi5'T:Hsap\MYC in vitro construct - coding region fusion 0 Yes piwibcd.3'UTR.T:Hsap\MYC in vitro construct - regulatory fusion 0 Yes piwidsRNA.cDa in vitro construct - RNAi 0 Yes piwidsRNA.shRNA.Scer\UAS.P\T in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes piwihs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes piwiRLD in vitro construct - genomic fragment 0 Yes piwiScer\UAS.P\T.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes piwiT:Zzzz\TAP in vitro construct - coding region fusion 0 Yes piwitCH322-103C03 in vitro construct - genomic fragment 0 Yes piwiV30A in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC145 (Christensen and Cook, 2006.3.8) Df(2L)BSC213 (Christensen and Cook, 2006.8.30) Df(2L)Δ37 (Zallen et al., 2002)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11827} NA P{KK105350} NA P{TRiP.HMS00185} NA P{TRiP.HMS00606} NA P{TRiP.JF01394} NA P{UASp-piwi.shRNA} NA heat-shock construct P{hs-myc-piwi} NA characterization construct P{5'-myc-piwi} NA P{piwi-3'-myc} NA P{piwi.RLD} NA P{piwi+.Rc12} NA P{piwiV30A} NA PBac{CH322-103C03} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}piwiEP1024 NA miscellaneous insertions P{ry11}piwi2 NA PBac{768.FSVS-0}piwiCPTI003588 NA PBac{RB}piwie00824 NA PBac{WH}piwif07053a NA insertion of enhancer trap P{PZ}piwi1 No P{PZ}piwi4 No P{PZ}piwi06843 No
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 18 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Dcr-1 (Megosh et al., 2006) inferred from physical interaction with Fmr1 (Megosh et al., 2006) inferred from physical interaction with vas (Megosh et al., 2006) RNA binding inferred from direct assay (Brennecke et al., 2007)
Biological Process
CV term Evidence References
chromatin silencing inferred from mutant phenotype (Pal-Bhadra et al., 2002, Pal-Bhadra et al., 2004, Grimaud et al., 2006) female germ-line stem cell division inferred from mutant phenotype (assigned by UniProtKB) (Cox et al., 1998) male germ-line stem cell division inferred from mutant phenotype (assigned by UniProtKB) (Cox et al., 1998) negative regulation of transposition, DNA-mediated inferred from mutant phenotype (Kalmykova et al., 2005) pole cell formation inferred from mutant phenotype (Megosh et al., 2006) positive regulation of nuclear-transcribed mRNA poly(A) tail shortening inferred from mutant phenotype (Rouget et al., 2010) posttranscriptional gene silencing inferred from mutant phenotype (Pal-Bhadra et al., 2002) stem cell division inferred from mutant phenotype (assigned by UniProtKB) (Cox et al., 1998)
Cellular Component
CV term Evidence References
chromocenter inferred from direct assay (Brower-Toland et al., 2007) cytoplasm inferred from direct assay (Zamparini et al., 2011) euchromatin inferred from direct assay (Brower-Toland et al., 2007) nucleoplasm inferred from direct assay (Lau et al., 2009) nucleus inferred from direct assay (Brennecke et al., 2007, Saito et al., 2006, Grimaud et al., 2006, Zamparini et al., 2011) polytene chromosome inferred from direct assay (Brower-Toland et al., 2007) telomeric heterochromatin inferred from direct assay (Brower-Toland et al., 2007) Yb body inferred from physical interaction with armi (Handler et al., 2011)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
mRNA binding non-traceable author statement (Morgan, 2002)
Biological Process
CV term Evidence References
cell fate determination traceable author statement (Deng and Lin, 2001) germ-line stem cell division traceable author statement (Deng and Lin, 2001, Lin, 2002) germ-line stem cell maintenance traceable author statement (Williams and Rubin, 2002) RNA interference traceable author statement (Cerutti, 2003)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Morgan, 2002) nucleoplasm non-traceable author statement (Deng and Lin, 2001) traceable author statement (Lin, 2002) nucleus traceable author statement (Cerutti, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions piwi allele Gene References piwi2 CG7816 (Smulders-Srinivasan and Lin, 2003) hh (King et al., 2001) l(3)99Da (Smulders-Srinivasan and Lin, 2003) l(3)99De (Smulders-Srinivasan and Lin, 2003) l(3)99Dg (Smulders-Srinivasan and Lin, 2003) l(3)99Ea (Smulders-Srinivasan and Lin, 2003) RpS3 (Smulders-Srinivasan and Lin, 2003) sima (Smulders-Srinivasan and Lin, 2003) Sry-δ (Smulders-Srinivasan and Lin, 2003) tgo (Smulders-Srinivasan and Lin, 2003) piwi12 sima (Smulders-Srinivasan and Lin, 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60588 DroID - A comprehensive database of gene and protein interactions. • FBgn0004872 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34521
Orthologs
Genome-wide drosophilid orthologs	Dere\GG10337 Dgri\GH10762 Dmoj\GI20575 Dpse\GA19370 Dsec\GM11236 Dsim\GD22206 Dvir\GJ13916 Dwil\GK14889 Dyak\GE13110
Curated drosophilid orthologs	Dpse\GA19370
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • 3723.m00019 Tetrahymena thermophila (ciliate protozoa) 95940.m00269 Trichomonas vaginalis (parasitic flagellated protozoan) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0230809 FBgn0216894 FBgn0201134 FBgn0193615 FBgn0166181 FBgn0079367 FBgn0156620 FBgn0143311 FBgn0118243 FBgn0102646 FBgn0091074
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	12225 P{PZ}piwi06843 cn1/CyO; ry506 33724 y1 sc* v1; P{TRiP.HMS00606}attP2 31610 y1 v1; P{TRiP.JF01394}attP2/TM3, Ser1 34866 y1 sc* v1; P{TRiP.HMS00185}attP2/TM3, Sb1
Harvard	- PBac{RB}piwie00824
Kyoto	108882 P{PZ}piwi06843 cn1/CyO; ry506
VDRC	v101658 P{KK105350}VIE-260B v22235 w1118; P{GD11827}v22235
Genomic Clones ( 2 )
	BACR03D06 BACR20O15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 82 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM05853 RE21038
Other clones	FI04411
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM28157 LD03506 LD33002 LD35072 RE03347 RE09253 RE38167 RE59653
Other clones	66976 CK01.20A.E2 CK01.65D.H11 CK01.77B.B10 Dmel_dig_020124_0682_2763 EC255169 EK018737 EK053630 EK060437 EK194863 FGC013E07 FGC016F11 FGC018H08 FGC023D06 FGM223G03 LD03429 RP003002961 RP003031502 RP003044337 RP003063400 RP003065591 RP003066862 RP003100732 RP003107169 RP003112286 RP003112809 RP003138007 RP003144984 RP003148061 RP003148850 RP003155672 RP003169527 RP003174414 RP003197232 RP003226106 RP003244531 RP003245574 RP003249834 RP003251111 RP003251233 RP003251745 RP003268780 RP003293612 RP003295923 RP003305605 RP003324054 RP003326121 RP003326431 RP003342844 RP003348712 RP003350678 RP003355680 RP003373722 RP003385843 RP003427138 RP003450984 RP003458459 RP003470980 RP003472094 RP003486770 RP003497034 RP003514111 RP003546473 RP003548383 RP003560537 RP003601404 RP003623035 RP003644990 RP003649045 RP003654044 RP003662280
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004872 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34521
Antibody Information
	polyclonal (Klattenhoff et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: piwi CG6122 (Gong, 1999.11)
Source for database merge of
Additional comments
Other Comments
	piwi is required for maintenance of long-distance chromosomal interactions between endogenous PcG target loci. (Grimaud et al., 2006) piwi protein is localised to the nucleus and is specifically associated with rasiRNAs (repeat-associated small RNAs) derived from various repetitive elements in the genome such as retrotransposons and heterochromatic regions in vivo and shows shows target RNA cleavage ("Slicer") activity in the presence of a single-stranded guide siRNA in vitro. (Saito et al., 2006) Expression is enriched in embryonic gonads. (Shigenobu et al., 2006) piwi is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs). (Vagin et al., 2006) The piwi product binds directly to repeat-associated small interfering RNAs (rasiRNAs). (Vagin et al., 2006) piwi is required for the silencing of retrotransposons in the male germline. (Kalmykova et al., 2005) bam is epistatic to somatic niche piwi function in the ovary, but germline piwi is epistatic to bam function in the ovary. (Szakmary et al., 2005) Mutations in piwi or aub result in partial loss of the methyl-K9 form of His3, most evident at minor sites within the euchromatic arms. (Pal-Bhadra et al., 2004) piwi, aub and spn-E are suppressors of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon. (Pal-Bhadra et al., 2004) piwi has a cell autonomous function in promoting stem cell division in the germline. (Cox et al., 2000) piwi mediates a somatic signalling mechanism required for the asymmetric division of germ-line stem cells to produce and maintain a daughter germ-line stem cell but is not essential for the further differentiation of the committed daughter cell. (Cox et al., 1998) Mutant analysis demonstrates the piwi function is required both to maintain germline stem cells and subsequently for the division and differentiation of the stem cell progeny in both sexes. Mutations abolish the proliferation ability of both female and male germline stem cells. (Lin and Spradling, 1997) piwi gene product affects the asymmetric division of the male and female germline stem cell (GSC). (Lin and Spradling, 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057527 RefSeq (Proteins) • NP_476875 DDBJ / EMBL / GenBank • AA802575 AAD08704 AAD08705 AAF53043 AC092226 AF104354 AF104355 AQ025014 AW941950 BI214891 CZ478664 Entrez Gene - A searchable database of RefSeq genes. • 34521 UniProtKB/Swiss-Prot • Q9VKM1 UniProtKB/TrEMBL • Q95SC7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Argonaute/Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165) Ribonuclease H-like (IPR012337)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60588 DroID - A comprehensive database of gene and protein interactions. • FBgn0004872 DRSC - Results from RNAi screens. • FBgn0004872 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004872 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4636-RA;CG6122-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004872 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34521 InParanoid A subset of ortholog calls from InParanoid. • 3723.m00019 Tetrahymena thermophila (ciliate protozoa) 95940.m00269 Trichomonas vaginalis (parasitic flagellated protozoan) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/piwi1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34521 modMine - Data generated by the modENCODE project. • FBgn0004872 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0230809 FBgn0216894 FBgn0201134 FBgn0193615 FBgn0166181 FBgn0079367 FBgn0156620 FBgn0143311 FBgn0118243 FBgn0102646 FBgn0091074
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG6122 (Keleman et al., 2009.8.5, Ni et al., 2008, Ni et al., 2010.12.1, Ni et al., 2011.7.19) Piwi (Czech et al., 2008, Zamore, 2007, Antoniewski et al., 2007, Lei, 2007, Nystul and Spradling, 2006, Dorner et al., 2006, Klenov et al., 2007, Prasanth and Spector, 2007, Gunawardane et al., 2007, Lin, 2007, Gunawardane et al., 2007, Zamore, 2006, Carthew, 2006, Lei et al., 2008, Shpiz et al., 2008, Yin and Lin, 2007, Saito et al., 2007, Kawamura et al., 2008, Peng and Karpen, 2007, Li et al., 2009, Nishida et al., 2007, Klattenhoff et al., 2009, Gim et al., 2001, Nishida et al., 2009, Kirino et al., 2010) PIWI (Grewal and Elgin, 2007, Lowell, 2000, Morgan, 2002, Grimaud et al., 2006, Megosh et al., 2006, Bai et al., 2007, Klenov, 2008, Lin, 2008, Rehwinkel et al., 2006, Mendez et al., 2011) piwi (Saito et al., 2006, Ronsseray et al., 2007, Kavi and Birchler, 2007, Glaser et al., 2007, Bhadra et al., 2007, Kruger, 2007, Zaratiegui, 2007, Lei and Corces, 2006, Vagin et al., 2006, Vagin et al., 2006, Megosh et al., 2006, Shigenobu et al., 2006, Christensen and Cook, 2006.8.30, Wu and Silverman, 2007, Desset et al., 2008, Parrott et al., 2011, Lau et al., 2009, Shpiz et al., 2007, Kalmykova et al., 2005, Pek and Kai, 2011, Haynes et al., 2006, Kavi et al., 2006, Brennecke et al., 2007, Payne and Braun, 2006, Kalmykova et al., 2008, Chau et al., 2008, Ronsseray et al., 2008, Kavi and Birchler, 2008, Gonzalez-Reyes, 2003, Simmons et al., 2007, Brower-Toland et al., 2007, Georlette et al., 2007, Josse et al., 2007, Xing et al., 2007, Yang et al., 2007, Berdnik et al., 2008, McGraw et al., 2008, Blumenstiel et al., 2008, Xie et al., 2005, Chau et al., 2009, Kirino et al., 2009, Szakmary et al., 2009, Malone et al., 2009, Shpiz et al., 2009, Habayeb et al., 2009, Chambeyron et al., 2008, Lei and Corces, 2006, Cross and Simmons, 2008, Robine et al., 2009, Venken et al., 2009, Saito et al., 2009, Lu and Clark, 2010, Fernández-Ayala et al., 2010, Moshkovich and Lei, 2010, Simmons et al., 2010, Olivieri et al., 2010, Li and Lu, 2009, Haase et al., 2010, Nagao et al., 2010, Janic et al., 2010, Jepson and Reenan, 2009, Gangaraju et al., 2011, Poulton et al., 2011, Ni et al., 2011, Pelisson et al., 2007, Saito et al., 2010, Handler et al., 2011, Liu et al., 2010, Stefanov et al., 2010, Rouget et al., 2010, Zamparini et al., 2011, Thomsen et al., 2010)
Name Synonym	P-element induced wimpy testis (Lin and Spradling, 1997) piwi (Kavi et al., 2006, Josse et al., 2007, Xing et al., 2007, Cross and Simmons, 2008, Saito et al., 2009) Piwi (Carmi, 2006) PIWI (Seydoux and Braun, 2006, Ronsseray et al., 2007)
Secondary FlyBase IDs
References ( 214 )
Generate a list of	All references relating to this gene ( 214 )
List References by type	Research paper  ( 98 ) Supplementary material  ( 5 ) Review  ( 57 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 34 ) FlyBase analysis  ( 3 ) Conference report  ( 4 ) Curated genome annotation  ( 1 ) Computer file  ( 3 )
Recent research papers ( 24 )
	Gangaraju et al., 2011, Nat. Genet. 43(2): 153--158 Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation. [FBrf0212873] Handler et al., 2011, EMBO J. 30(19): 3977--3993 A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. [FBrf0216344] Mendez et al., 2011, Chembiochem 12(7): 1084--1096 The HP1a Disordered C Terminus and Chromo Shadow Domain Cooperate to Select Target Peptide Partners. [FBrf0213514] Moshkovich et al., 2011, Genes Dev. 25(16): 1686--1701 RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. [FBrf0214686] Ni et al., 2011, Nat. Methods 8(5): 405--407 A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581] Parrott et al., 2011, PLoS ONE 6(9): e25087 Nucleoporin98-96 Function Is Required for Transit Amplification Divisions in the Germ Line of Drosophila melanogaster. [FBrf0216255] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Poulton et al., 2011, Development 138(9): 1737--1745 The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. [FBrf0213494] Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711 Mechanism of the piRNA-mediated silencing of Drosophila telomeric retrotransposons. [FBrf0216525] Zamparini et al., 2011, Development 138(18): 4039--4050 Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. [FBrf0214783] Fernández-Ayala et al., 2010, PLoS ONE 5(1): e8549 Gene expression in a Drosophila model of mitochondrial disease. [FBrf0209705] Haase et al., 2010, Genes Dev. 24(22): 2499--2504 Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila. [FBrf0212277] Janic et al., 2010, Science 330(6012): 1824--1827 Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila. [FBrf0212713] Kirino et al., 2010, RNA 16(1): 70--78 Arginine methylation of Aubergine mediates Tudor binding and germ plasm localization. [FBrf0209562] Liu et al., 2010, Genes Dev. 24(17): 1876--1881 Structural basis for methylarginine-dependent recognition of Aubergine by Tudor. [FBrf0211676] Lu and Clark, 2010, Genome Res. 20(2): 212--227 Population dynamics of PIWI-interacting RNAs (piRNAs) and their targets in Drosophila. [FBrf0209834] Moshkovich and Lei, 2010, PLoS Genet. 6(3): e1000880 HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila. [FBrf0210291] Nagao et al., 2010, RNA 16(12): 2503--2515 Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis. [FBrf0212366] Olivieri et al., 2010, EMBO J. 29(19): 3301--3317 An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila. [FBrf0212004] Rouget et al., 2010, Nature 467(7319): 1128--1132 Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. [FBrf0212183] Saito et al., 2010, Genes Dev. 24(22): 2493--2498 Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. [FBrf0212297] Simmons et al., 2010, Genet. Res. (Camb.) 92(4): 261--272 Maternal impairment of transposon regulation in Drosophila melanogaster by mutations in the genes aubergine, piwi and Suppressor of variegation 205. [FBrf0212081] Stefanov et al., 2010, Mol. Genet. Genomics 284(5): 373--381 Amplification of "defective" retrotransposon gtwin in D. melanogaster strain carrying large complex chromosomal aberration. [FBrf0212070] Thomsen et al., 2010, Genome Biol. 11(9): R93 Genome-wide analysis of mRNA decay patterns during early Drosophila development. [FBrf0212195] Show all research papers ...
Recent reviews ( 1 )
	Arkov and Ramos, 2010, Trends Cell Biol. 20(8): 482--490 Building RNA-protein granules: insight from the germline. [FBrf0212430] Show all reviews ...