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General Information
Symbol
Dmel\bowl
Species
D. melanogaster
Name
brother of odd with entrails limited
Annotation Symbol
CG10021
Feature Type
FlyBase ID
FBgn0004893
Gene Model Status
Stock Availability
Gene Summary
brother of odd with entrails limited (bowl) encodes a member of the Odd-skipped (Odd) family of zinc finger proteins involved in developmental patterning and cell fate specification. Its localization in the nucleus is regulated by the nuclear protein encoded by lin and the the product of drm with opposing roles. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
Also Known As

bowel, Su(tor)2-1

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-11
RefSeq locus
NT_033779 REGION:3771700..3784121
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with FLYBASE:odd; FB:FBgn0002985
inferred from biological aspect of ancestor with PANTHER:PTN000367524
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000367524
(assigned by GO_Central )
Biological Process (13 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002306374
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002306374
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:P23803
(assigned by UniProt )
inferred from sequence or structural similarity with UniProtKB:P23803
(assigned by UniProt )
inferred from sequence or structural similarity with UniProtKB:P23803
(assigned by UniProt )
inferred from sequence or structural similarity with UniProtKB:P23803
(assigned by UniProt )
inferred from biological aspect of ancestor with PANTHER:PTN000367524
(assigned by GO_Central )
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000367524
(assigned by GO_Central )
located_in nucleus
inferred from sequence or structural similarity with FLYBASE:odd; FB:FBgn0002985
Protein Family (UniProt)
-
Summaries
Gene Snapshot
brother of odd with entrails limited (bowl) encodes a member of the Odd-skipped (Odd) family of zinc finger proteins involved in developmental patterning and cell fate specification. Its localization in the nucleus is regulated by the nuclear protein encoded by lin and the the product of drm with opposing roles. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -
Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys2His2 zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).
Protein Function (UniProtKB)
Putative transcription factor. Required for leg joint formation, acting downstream of Notch to pattern the leg tarsal segments. Functions in the terminal pathway during embryogenesis, acting downstream of tll in the posterior of the embryo. Acts in a hierarchy downstream of drm and lin during foregut and hindgut patterning and morphogenesis. Involved in cell rearrangement during elongation of the embryonic hindgut. Regulates expression of hindgut patterning genes to establish the small intestine region of the embryonic hindgut. Required in the foregut for spatially localized gene expression and morphogenesis of the proventriculus.
(UniProt, Q9VQU9)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\bowl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VQU9)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0077493
3485
744
FBtr0077490
3513
744
FBtr0077491
3424
744
FBtr0077492
3630
744
FBtr0307027
3507
744
FBtr0307028
3394
744
FBtr0307029
3435
744
Additional Transcript Data and Comments
Reported size (kB)

3.4 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
FBpp0077182
79.8
744
7.20
FBpp0077179
79.8
744
7.20
FBpp0077180
79.8
744
7.20
FBpp0077181
79.8
744
7.20
FBpp0297870
79.8
744
7.20
FBpp0297871
79.8
744
7.20
FBpp0297872
79.8
744
7.20
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)

744 (aa); 80 (kD predicted)

Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bowl using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
anterior endoderm anlage

Comment: anlage in statu nascendi

posterior ectoderm anlage

Comment: anlage in statu nascendi

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states >= 2 hr AEL

Additional Descriptive Data

bowl is expressed in the antennal disc during larval stages. In L1, it is expressed in many cells of the disc. In late L2, early L3, it is expressed in the ventral portion of the disc but excluded from the appendage region. The region extends posteriorly to the ventral limit between the antennal and eye portions of the disc and includes the prospective maxillary palp and ventral head regions. In late L3, bowl expression resolves into two rings in the antennal region and a stripe in the maxillary palp region of the disc. The pattern is coincident with that of Ecol\lacZodd-rK111.

Transcript is observed in a segmentally repeated pattern in late third instar leg discs. Expression is observed in each presumptive leg segment including the tarsi. A broad stripe of expression is observed in each presumptive segment manifest as concentric rings. Expression is maintained in the everting pupal leg.

The anterior domain of bowl expression falls in two bands, one forms an anterior cap, the other forms a band between approximately 15 and 20% egg length. The two anterior domains are connected by solid ventral expression.

bowl transcripts accumulate in three domains in the cellular blastoderm embryo. The domains are a strong posterior cap from 0-11% egg length, a weak and nonuniform anterior domain from 84-100% egg length, and a broad transverse stripe (~6 cells wide) that lies just anterior to the presumptive cephalic furrow. At early gastrulation, expression is observed in seven pair-rule stripes that quickly evolve into a segment polarity pattern.

bowl transcripts are expressed in embryos from 2-4hrs on with a peak in 8-12hr embryos. bowl transcripts are detected in three distinct domains at the cellular blastoderm stage by in situ hybridization. They are found at the anterior and posterior poles and in a transverse stripe, approximately 6 cells wide, centered at 67% egg length at the dorsal midline and at 76% egg length at the ventral midline. During gastrulation and germ band extension, the posterior domain cells follow the amnioproctodeal invagination and are expressed in the posterior midgut and hindgut. The anterior domain cells invaginate with the anterior midgut. Some of the cells in the transverse stripe become part of the cephalic furrow. Later, cells in the invaginating stomodeum and cells that will give rise to the clypeolabrum express bowl. By germband retraction, expression in the hindgut and posterior midgut decreases while expression in the anterior midgut and foregut persists.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

bowl protein is localized to proximal half of the true leg segments of leg discs; localization is distal to that of Dl and nub.

bowl is expressed in the antennal disc during larval stages. In L1, it is expressed in many cells of the disc. In late L2, early L3, it is expressed in the ventral portion of the disc but excluded from the appendage region. The region extends posteriorly to the ventral limit between the antennal and eye portions of the disc and includes the prospective maxillary palp and ventral head regions. In late L3, bowl expression resolves into two rings in the antennal region and a stripe in the maxillary palp region of the disc.

bowl protein accumumates in peripodial epithelial cells in the wing disc from the second larval instar. At early to mid third instar, bowl continues to be expressed in the peripodial epithelium, and at the lateral margins of the presumptive notum. bowl protein is absent from the disc epithelium proper in the wing disc. Late in larval development, bowl is downregulated in the medial region of the peripodial epithelium, and absent from its posterior margin. In everting discs in early pupae, bowl protein is detected at high levels in the ventro-anterior hinge, and at the lateral margins of the presumptive notum

Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Reporter: P{lacW}k08617
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\bowl in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 21 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of bowl
Transgenic constructs containing regulatory region of bowl
Aberrations (Deficiencies and Duplications) ( 9 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
adult cuticle & leg | somatic clone
peripodial epithelium & dorsal mesothoracic disc | somatic clone | first instar larval stage
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
No
4 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
Yes
4 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (2)
5 of 13
Yes
No
3 of 13
No
No
Xenopus tropicalis (Western clawed frog) (8)
5 of 12
Yes
Yes
4 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (12)
6 of 15
Yes
Yes
4 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
6 of 15
Yes
No
5 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (3)
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (2)
1 of 12
Yes
No
1 of 12
Yes
No
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (6)
5 of 10
3 of 10
2 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-11
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    24C3-24C4
    Limits computationally determined from genome sequence between P{EP}EP2595EP2595 and P{lacW}edk01102
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    24C-24C
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (18)
    Genomic Clones (6)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (113)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: bowl CG10021

      Source for database merge of

      Source for merge of: bowl Su(tor)2-1

      Additional comments
      Other Comments

      Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.

      lin and bowl act as a binary switch to specify disc proper and peripodial epithelium fates respectively during early development of the wing primordium.

      bowl mutants show defects in leg morphogenesis; clones result in a failure of joint formation from the distal tibia to tarsal segment 5, while more proximal clones cause melanotic protrusions from the leg cuticle.

      bowl is required for patterning of the hindgut.

      cad acts in hindgut development through fog, fkh and wg, but does not play a role in activating tll, hkb, byn and bowl which are also required for proper hindgut development.

      odd, sob and bowl encode closely related zinc finger proteins. The distinct expression of bowl, less tightly linked to odd than sob, and analysis of bowl mutations (FBrf0089187) indicates a more divergent developmental role.

      Molecular and genetic analysis of bowl reveals the functions of bowl during embryogenesis are distinct from odd despite the structural similarities between the two genes.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 63 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      modMine - A data warehouse for the modENCODE project
      Linkouts
      ApoDroso - Functional genomic database for photoreceptor development, survival and function
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (11)
      Reported As
      Symbol Synonym
      org2
      Secondary FlyBase IDs
      • FBgn0022034
      • FBgn0014041
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      Study result (0)
      Result
      Result Type
      Title
      References (130)