leo, 14-3-3, leonardo, 549, par-5
a 14-3-3zeta isoform - a phosphoserine/threonine interacting protein - modifies ras pathway signaling and facilitating olfactory learning - 14-3-3 proteins regulate Tctp-Rheb interaction for organ growth
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Homodimer; homodimerization is not essential for modulating the activity of Slo (PubMed:12529354). Interacts with phosphorylated Slob; the interaction with Slob mediates an indirect interaction with Slo (PubMed:10230800). Interacts with phosphorylated yki (PubMed:18256197, PubMed:19900439). Interacts with hemo; this represses 14-3-3zeta activity which prevents the 14-3-3zeta-mediated activation of phosphoinositide 3-kinase Pi3K68D. This, in turn, inhibits the Pi3K68D-mediated conversion of phosphatidylinositol to phosphatidylinositol-3-phosphate and prevents progression of early endosomes through the maturation process which regulates subsequent steps of phagocytic processing (PubMed:27015288). Interacts with REPTOR (when phosphorylated), this interaction may assist the cytoplasmic retention of REPTOR (PubMed:25920570).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\14-3-3ζ using the Feature Mapper tool.
The 2.9kb 14-3-3ζ transcripts aredetected mainly in RNA from mid to late embryos and in adult head but notbody RNA. In situ hybridization in embryos shows that transcripts areabundant along the ventral nerve cord and less prominant elsewhere. In theadult head, transcripts are detected in regions of the optic lobe, laminaand retina. The probe used for in situ hybridization detects all three14-3-3ζ transcripts.
14-3-3ζ protein is moderately expressed in the mushroom body alpha', beta', alpha, beta and gamma lobes, in the spur and in the calyx.
14-3-3ζ protein is first detected in the nervous system in 16-18hr embryos. It is observed in cell bodies, axons and synapses, including the neuromuscular junction. Over the next few hours it is partitioned to the neuromuscular junction and lost from the motor axons. By 20 hours, it is mainly in the synaptic boutons and is barely detectable in axons. In larvae, 14-3-3ζ protein is highly enriched in neuromuscular junction synaptic boutons. It is found at lower levels in surrounding tissues including the motor axons and muscle. It is expressed in both type I and type II neuromuscular junctions. It was found to be enriched in presynaptic boutons.
14-3-3ζ protein is expressed in most if not all cells of the eye imaginal disc. It is enriched in the region posterior to the morphogenetic furrow.
GBrowse - Visual display of RNA-Seq signalsView Dmel\14-3-3ζ in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Both isoforms of the 14-3-3ζ gene are required acutely (as opposed to developmentally) for normal learning and memory.
14-3-3ζ is required for normal chromosome separation during syncytial mitoses in the embryo.
14-3-3ζ is strongly and specifically expressed in the presynaptic boutons of the neuro muscular junction.
In mutants the basic processes of synaptogenesis and excitation-secretion coupling are not perturbed, but properties of synaptic modulation such as transmission augmentation, high frequency transmission fidelity and post-tetanic potentiation (PTP) are strongly impaired.
14-3-3ζ may function in the activity-dependent regulation of synaptic vesicle dynamics to control the pool of releaseable transmitter vesicles at presynaptic fusion sites.
14-3-3ζ has a biological role in mushroom body-mediated learning and memory processes.
Complementation group identified in an EMS and DEB screen to isolate deficiencies that uncover Jra.
The alternate 5' end of Egfr reported by Schejter et al. (Cell 46: 1091--1101) is a cloning artefact and is actually from D14-3-3 of Swanson and Ganguly (Gene 113: 183--190).
D14-3-3 has been characterized: gene expression is developmentally regulated and predominantly expressed in the neural tissues of the fly.
Identified as a cDNA clone that is expressed exclusively or predominantly in the adult visual system.
Developmental expression pattern of the cDNA clone is examined.
Identified as a cDNA clone that is expressed at a low frequency in the body but abundantly in the head of the adult.