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General Information
Symbol
Dmel\14-3-3ζ
Species
D. melanogaster
Name
14-3-3ζ
Annotation Symbol
CG17870
Feature Type
FlyBase ID
FBgn0004907
Gene Model Status
Stock Availability
Gene Snapshot
14-3-3ζ (14-3-3ζ) encodes three acidic protein isoforms due to alternative splicing. The isoforms homo- and heterodimerize with each other and other members of the family. It functions in multiple signaling pathways, most prominently in the Ras/MAPK cascade. It is involved in epithelial cell polarity, development of the eye, embryogenesis and in adult associative learning. [Date last reviewed: 2019-03-07]
Also Known As
leo, 14-3-3, leonardo, 549, 14-3-3 zeta
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:10,099,677..10,109,687 [+]
Recombination map
2-62
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the 14-3-3 family. (P29310)
Summaries
Gene Group (FlyBase)
14-3-3 PROTEINS -
The 14-3-3 proteins are a family of highly conserved acidic 30 kDa homo/heterodimeric adapter proteins that bind and regulate protein activity. (Adapted from FBrf0201747, FBrf0194430 and PMID:24564655).
Pathway (FlyBase)
Positive Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Positive regulators of the pathway, enhance the cytosolic retention of yki, negatively regulating tissue growth (Adapted from FBrf0224870).
Positive Regulators of Torso Signaling Pathway -
Positive regulators of Torso signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl).
Protein Function (UniProtKB)
Required in Raf-dependent cell proliferation and photoreceptor differentiation during eye development (PubMed:9159395). Acts upstream of Raf and downstream of Ras, and is essential for viability (PubMed:9159395). Acts as a negative regulator of the slo calcium channel via its interaction with slo-binding protein slob (PubMed:10230800). Inhibits yki activity by restricting its nuclear localization (PubMed:19900439). Binds to and promotes the activity of phosphoinositide 3-kinase Pi3K68D which converts phosphatidylinositol to phosphatidylinositol-3-phosphate and promotes maturation of early endosomes (PubMed:27015288).
(UniProt, P29310)
Summary (Interactive Fly)
a 14-3-3zeta isoform - a phosphoserine/threonine interacting protein - modifies ras pathway signaling and facilitating olfactory learning - 14-3-3 proteins regulate Tctp-Rheb interaction for organ growth
Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\14-3-3ζ or the JBrowse view of Dmel\14-3-3ζ for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088413
1753
248
FBtr0088412
3078
248
FBtr0088419
2966
248
FBtr0088414
2966
248
FBtr0088415
3078
248
FBtr0088416
1753
248
FBtr0088417
3046
248
FBtr0088418
2966
248
FBtr0100182
974
248
FBtr0100183
1753
248
FBtr0332916
2924
248
FBtr0332917
1760
248
Additional Transcript Data and Comments
Reported size (kB)
2.9, 1.9, 1.0 (northern blot)
2.9, 1.0 (unknown); 1.9 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087501
28.3
248
4.56
FBpp0087500
28.3
248
4.56
FBpp0089338
28.3
248
4.74
FBpp0087502
28.2
248
4.50
FBpp0087503
28.2
248
4.50
FBpp0087504
28.3
248
4.74
FBpp0087505
28.3
248
4.56
FBpp0089337
28.3
248
4.56
FBpp0099539
28.3
248
4.56
FBpp0099540
28.2
248
4.50
FBpp0305136
28.2
248
4.50
FBpp0305137
28.2
248
4.50
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

248 aa isoforms: 14-3-3ζ-PC, 14-3-3ζ-PF
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Homodimer; homodimerization is not essential for modulating the activity of Slo (PubMed:12529354). Interacts with phosphorylated Slob; the interaction with Slob mediates an indirect interaction with Slo (PubMed:10230800). Interacts with phosphorylated yki (PubMed:18256197, PubMed:19900439). Interacts with hemo; this represses 14-3-3zeta activity which prevents the 14-3-3zeta-mediated activation of phosphoinositide 3-kinase Pi3K68D. This, in turn, inhibits the Pi3K68D-mediated conversion of phosphatidylinositol to phosphatidylinositol-3-phosphate and prevents progression of early endosomes through the maturation process which regulates subsequent steps of phagocytic processing (PubMed:27015288). Interacts with REPTOR (when phosphorylated), this interaction may assist the cytoplasmic retention of REPTOR (PubMed:25920570).
(UniProt, P29310)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\14-3-3ζ using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (24 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:Slob; FB:FBgn0264087
inferred from physical interaction with UniProtKB:Q45VV3
(assigned by UniProt )
inferred from physical interaction with UniProtKB:A0A0B4K753
(assigned by UniProt )
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000308
(assigned by InterPro )
Biological Process (13 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
non-traceable author statement
non-traceable author statement
Cellular Component (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
non-traceable author statement
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
The 2.9kb 14-3-3ζ transcripts aredetected mainly in RNA from mid to late embryos and in adult head but notbody RNA. In situ hybridization in embryos shows that transcripts areabundant along the ventral nerve cord and less prominant elsewhere. In theadult head, transcripts are detected in regions of the optic lobe, laminaand retina. The probe used for in situ hybridization detects all three14-3-3ζ transcripts.
The 1.0kb 14-3-3ζ transcript is mostabundant in early embryos. It is enriched in RNA from female vs. male andin RNA from ovary vs. body minus ovary. It is therefore assumed to bematernally inherited.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
cell body

Comment: reference states 16-18 hr AEL

mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Expression of 14-3-3ζ is within the presumptive mushroom body neuroblasts.
14-3-3ζ protein is moderately expressed in the mushroom body alpha', beta', alpha, beta and gamma lobes, in the spur and in the calyx.
14-3-3ζ protein is first detected in the nervous system in 16-18hr embryos. It is observed in cell bodies, axons and synapses, including the neuromuscular junction. Over the next few hours it is partitioned to the neuromuscular junction and lost from the motor axons. By 20 hours, it is mainly in the synaptic boutons and is barely detectable in axons. In larvae, 14-3-3ζ protein is highly enriched in neuromuscular junction synaptic boutons. It is found at lower levels in surrounding tissues including the motor axons and muscle. It is expressed in both type I and type II neuromuscular junctions. It was found to be enriched in presynaptic boutons.
14-3-3ζ protein is expressed in most if not all cells of the eye imaginal disc. It is enriched in the region posterior to the morphogenetic furrow.
14-3-3ζ protein is apically distributed in developing photoreceptor cells.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\14-3-3ζ in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 32 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 26 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of 14-3-3ζ
Transgenic constructs containing regulatory region of 14-3-3ζ
Deletions and Duplications ( 13 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
 
13 of 15
Yes
Yes
7 of 15
No
Yes
7 of 15
No
Yes
 
7 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
12 of 15
No
Yes
7 of 15
No
Yes
7 of 15
No
Yes
7 of 15
No
Yes
5 of 15
No
No
4 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (8)
13 of 13
Yes
Yes
12 of 13
No
Yes
7 of 13
No
Yes
7 of 13
No
Yes
5 of 13
No
Yes
5 of 13
No
Yes
5 of 13
No
Yes
4 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
11 of 12
Yes
Yes
9 of 12
No
Yes
6 of 12
No
Yes
5 of 12
No
Yes
5 of 12
No
Yes
2 of 12
No
No
Danio rerio (Zebrafish) (11)
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
9 of 15
No
Yes
9 of 15
No
Yes
8 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
7 of 15
No
Yes
5 of 15
No
No
5 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
13 of 15
Yes
Yes
12 of 15
No
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (14)
4 of 9
Yes
No
4 of 9
Yes
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
3 of 9
No
No
2 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
9 of 15
Yes
No
8 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
6 of 12
Yes
No
6 of 12
Yes
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190CIK )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150A0S )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0B92 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0B6P )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0VKY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (1)
7 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 5 )
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homodimer; homodimerization is not essential for modulating the activity of Slo (PubMed:12529354). Interacts with phosphorylated Slob; the interaction with Slob mediates an indirect interaction with Slo (PubMed:10230800). Interacts with phosphorylated yki (PubMed:18256197, PubMed:19900439). Interacts with hemo; this represses 14-3-3zeta activity which prevents the 14-3-3zeta-mediated activation of phosphoinositide 3-kinase Pi3K68D. This, in turn, inhibits the Pi3K68D-mediated conversion of phosphatidylinositol to phosphatidylinositol-3-phosphate and prevents progression of early endosomes through the maturation process which regulates subsequent steps of phagocytic processing (PubMed:27015288). Interacts with REPTOR (when phosphorylated), this interaction may assist the cytoplasmic retention of REPTOR (PubMed:25920570).
    (UniProt, P29310 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    Positive Regulators of Hippo Signaling Pathway -
    The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Positive regulators of the pathway, enhance the cytosolic retention of yki, negatively regulating tissue growth (Adapted from FBrf0224870).
    Positive Regulators of Torso Signaling Pathway -
    Positive regulators of Torso signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl).
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-62
    Cytogenetic map
    Sequence location
    2R:10,099,677..10,109,687 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    46E6-46E8
    Limits computationally determined from genome sequence between P{lacW}Adamk13906&P{EP}Pka-R2EP2162 and P{PZ}14-3-3ζ07103
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    46E4-46E8
    (determined by in situ hybridisation)
    46E-46F
    (determined by in situ hybridisation)
    Complementation data from unspecified deficiency chromosomes.
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (19)
    Genomic Clones (27)
    cDNA Clones (581)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Source for merge of: 14-3-3ζ l(2)46Ee
      Source for merge of: 14-3-3ζ l(2)46CFe
      Source for merge of: 14-3-3ζ BEST:GH05075
      Additional comments
      Other Comments
      Both isoforms of the 14-3-3ζ gene are required acutely (as opposed to developmentally) for normal learning and memory.
      14-3-3ζ is required for normal chromosome separation during syncytial mitoses in the embryo.
      14-3-3ζ can interact with and modulate slo via Slob. The binding between 14-3-3ζ and Slob is regulated by calcium/calmodulin-dependent kinase II phosphorylation.
      14-3-3ζ is strongly and specifically expressed in the presynaptic boutons of the neuro muscular junction.
      In mutants the basic processes of synaptogenesis and excitation-secretion coupling are not perturbed, but properties of synaptic modulation such as transmission augmentation, high frequency transmission fidelity and post-tetanic potentiation (PTP) are strongly impaired.
      14-3-3ζ may function in the activity-dependent regulation of synaptic vesicle dynamics to control the pool of releaseable transmitter vesicles at presynaptic fusion sites.
      Genetic studies indicate that 14-3-3ζ acts downstream of Ras85D and upstream of phl in the developing eye disc.
      14-3-3ζ has a biological role in mushroom body-mediated learning and memory processes.
      Complementation group identified in an EMS and DEB screen to isolate deficiencies that uncover Jra.
      The alternate 5' end of Egfr reported by Schejter et al. (Cell 46: 1091--1101) is a cloning artefact and is actually from D14-3-3 of Swanson and Ganguly (Gene 113: 183--190).
      D14-3-3 has been characterized: gene expression is developmentally regulated and predominantly expressed in the neural tissues of the fly.
      Identified as a cDNA clone that is expressed exclusively or predominantly in the adult visual system.
      Developmental expression pattern of the cDNA clone is examined.
      Identified as a cDNA clone that is expressed at a low frequency in the body but abundantly in the head of the adult.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 137 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      modMine - A data warehouse for the modENCODE project
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (38)
      Reported As
      Symbol Synonym
      14-3-3-Leo
      14-3-3-zeta
      14-3-3ZETA
      4-3-3 zeta
      BEST:GH05075
      D14-3-3
      d14-3-3zeta
      l(2)07103
      l(2)46CFe
      l(2)46Ee
      Secondary FlyBase IDs
      • FBgn0010635
      • FBgn0019723
      • FBgn0023038
      • FBgn0046306
      • FBgn0064146
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (257)