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Gene Dmel\TfIIB

General Information
Symbol	Dmel\TfIIB	Species	D. melanogaster
Name	Transcription factor IIB	Annotation symbol	CG5193
Feature type	protein_coding_gene	FlyBase ID	FBgn0004915
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	dTFIIB, dmTFIIB
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	31E2-31E2	Sequence location	2L:10,431,338..10,433,116 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Transcription factor IIB is referred to in FlyBase by the symbol Dmel\TfIIB (CG5193, FBgn0004915). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: transcription factor binding. There is experimental evidence that it is involved in the biological process: transcriptional start site selection at RNA polymerase II promoter; regulation of DNA binding; regulation of transcription from RNA polymerase II promoter. 37 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Cyclin-like; Transcription factor TFIIB; Transcription factor TFIIB, conserved site; Transcription factor TFIIB, cyclin-like domain; Zinc finger, TFIIB-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1634172_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of TfIIB. Gene sequence location is 2L:10431338..10433116.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 31E2-31E2   Limits computationally determined from genome sequence between P{lacW}KdelRk00311 and P{lacW}l(2)k09116k09116&P{lacW}Fatpk10307
Experimentally Determined Cytological Location
Cytogenetic map Notes References 31F-31F   (determined by in situ hybridisation)   (Yamashita et al., 1992) 31F-31F   (determined by in situ hybridisation)   (Wampler and Kadonaga, 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\TfIIB for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) TfIIB-RA FBtr0080025  NM_057540   1410   315 TfIIB-RB FBtr0333489  NM_001273420   1604   315
Additional Transcript Data & Comments
Reported size (kB)	1.6, 1.3 (northern blot) (Yamashita et al., 1992) 1.5, 1.3 (northern blot) (Wampler and Kadonaga, 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein TfIIB-PA   FBpp0079615   34.4   315   8.40     NP_476888   AAF52951 TfIIB-PB   FBpp0305673   34.4   315   8.40     NP_001260349   AGB92884
Additional Polypeptide Data & Comments
Reported size (kDa)	315 (aa); 34 (kD predicted) (Yamashita et al., 1992, Wampler and Kadonaga, 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor TFIIB (IPR000812) Zinc finger, TFIIB-type (IPR013137) Transcription factor TFIIB, cyclin-like domain (IPR013150) Cyclin-like (IPR013763) Transcription factor TFIIB, conserved site (IPR023486)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141956   AA246683   AA263552   AA439549   AA541079   AA696704   AE014134 AAF52951   AC007451   AE014134 AGB92884   AI534957   AI546494   BF491801   BF497778   BF501368   BF501589   BI234090   BI372116   BI581127   BI607719   BP556428   BP556429   BT011459   BT015269 AAT94498   BX062484   CA806575   CO153906   CO180818   CO270212   CO274064   CO279776   CO286481   CO288149   CO289192   CO289550   CO293998   CO294099   CO296745   CO299249   CO300406   CO302007   CO307162   CO307603   CO312851   CO312912   CO312938   CO321872   CO333123   EC063887   EC066942   EC067698   EC067903   EC072037   EC074001   EC081528   EC081652   EC082403   EC087233   EC088970   EC089576   EC195282   EC196933   EC199942   EC200413   EC201397   EC206001   EC208346   EC219249   EC225507   EC225510   EC226399   EC228542   EC233714   EC239532   EC250603   EC263717   ES695559   ES695580   ES695593   ES696360   ES696364   ES696384   ES696385   ES696390   ES696393   ES696401   ES696414   ES696418   ES696419   ES696440   ES696451   EV600712   GH755403   GH756926   GH760437   GH785685   GH792952   GH793008   GH794339   GH795437   GH796025   GH801702   GH807060   GH807837   GH809603   GH815770   GH815879   GH827907   GH836449   GH839760   GH845313   GH848249   GH858723   GH869549   GH876192   GH899584   GH899623   GH912544   GH917591   GH926777   GH929909   GH936701   GH937628   M88164 AAA28930   M91081 AAA28929   U02879 AAA68626   U35148 AAA79093
UniProtKB/Swiss-Prot	P29052
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Wampler and Kadonaga, 1992)
Additional Descriptive Data
	TfIIB transcripts are expressed in embryos. (Wampler and Kadonaga, 1992) The 1.3kb TfIIB transcript is a minor transcript expressed in embryos. (Wampler and Kadonaga, 1992)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Wampler and Kadonaga, 1992)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    706.575 Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary    674.8 Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (706.575) Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary  (674.8) Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    706.575 Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary    674.8 Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    706.575 Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary    674.8 Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    706.575 Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary    674.8 Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  706.575 Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary  674.8 Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    706.575 Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary    674.8 Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    706.575 Larval Midgut    239 Larval Hindgut    281.9 Larval Malpighian Tubules    297.5 Larval Fat Body    302.1 Larval Salivary Gland    460.2 Larval Trachea    407.25 Larval Carcass    364.45 Adult Head    314.5 Adult Eye    245.2 Adult Brain    383.3 Adult Thoracic-Abdominal Ganglion    368 Adult Crop    383.9 Adult Midgut    263.9 Adult Hindgut    231.7 Adult Malpighian Tubules    494.8 Adult Fat Body    301.5 Adult Salivary Gland    320.7 Adult Heart    291.925 Adult VirginFemale Spermatheca    355.5 Adult InseminatedFemale Spermatheca    428.1 Adult Ovary    674.8 Adult Testis    356.1 Adult Male Accessory Gland    256.4 Adult Carcass    269.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    314.5   NA  Eye    245.2   NA  Brain    383.3   706.575  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    368   NA  Crop    383.9   239  Midgut    263.9   281.9  Hindgut    231.7   297.5  Malpighian Tubules    494.8   302.1  Fat Body    301.5   460.2  Salivary Gland    320.7   NA  Heart    291.925   407.25  Trachea    NA   NA  VirginFemale Spermatheca    355.5   NA  InseminatedFemale Spermatheca    428.1   NA  Ovary    674.8   NA  Testis    356.1   NA  Male Accessory Gland    256.4   364.45  Carcass    269.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    82 central nervous system, larvae L3    36 central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering    28 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female    55 ovary, mated 4-day female    44 testis, mated 4-day male    83 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (82) central nervous system, larvae L3  (36) central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering  (28) carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female  (55) ovary, mated 4-day female  (44) testis, mated 4-day male  (83) accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    82 central nervous system, larvae L3    36 central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering    28 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female    55 ovary, mated 4-day female    44 testis, mated 4-day male    83 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    82 central nervous system, larvae L3    36 central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering    28 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female    55 ovary, mated 4-day female    44 testis, mated 4-day male    83 accessory gland, mated 4-day male    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    82 central nervous system, larvae L3    36 central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering    28 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female    55 ovary, mated 4-day female    44 testis, mated 4-day male    83 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (82) central nervous system, larvae L3  (36) central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering  28 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female  (55) ovary, mated 4-day female  (44) testis, mated 4-day male  (83) accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    82 central nervous system, larvae L3    36 central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering    28 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female    55 ovary, mated 4-day female    44 testis, mated 4-day male    83 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    82 central nervous system, larvae L3    36 central nervous system, pupae P8    22 head, virgin 1-day female    12 head, virgin 4-day female    12 head, virgin 20-day female    13 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    11 head, mated 1-day male    15 head, mated 4-day male    18 head, mated 20-day male    22 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    19 digestive system, 1-day adult    15 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    5 fat body, white prepupae    20 fat body, pupae P8    20 carcass, larvae L3 wandering    28 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    11 ovary, virgin 4-day female    55 ovary, mated 4-day female    44 testis, mated 4-day male    83 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    125 embryo 04-06hr    100 embryo 06-08hr    91 embryo 08-10hr    93 embryo 10-12hr    110 embryo 12-14hr    66 embryo 14-16hr    42 embryo 16-18hr    39 embryo 18-20hr    31 embryo 20-22hr    47 embryo 22-24hr    42 larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    40 white prepupae new    42 white prepupae 12hr    40 white prepupae 24hr    48 pupae 2d postWPP    40 pupae 3d postWPP    28 pupae 4d postWPP    17 adult male 01day    27 adult male 05day    36 adult male 30day    28 adult female 01day    24 adult female 05day    41 adult female 30day    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (73) embryo 02-04hr  (125) embryo 04-06hr  (100) embryo 06-08hr  (91) embryo 08-10hr  (93) embryo 10-12hr  (110) embryo 12-14hr  (66) embryo 14-16hr  (42) embryo 16-18hr  (39) embryo 18-20hr  (31) embryo 20-22hr  (47) embryo 22-24hr  (42) larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (40) white prepupae new  (42) white prepupae 12hr  (40) white prepupae 24hr  (48) pupae 2d postWPP  (40) pupae 3d postWPP  (28) pupae 4d postWPP    17 adult male 01day  (27) adult male 05day  (36) adult male 30day  (28) adult female 01day    24 adult female 05day  (41) adult female 30day  (45) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr  (125) embryo 04-06hr    100 embryo 06-08hr    91 embryo 08-10hr    93 embryo 10-12hr  (110) embryo 12-14hr    66 embryo 14-16hr    42 embryo 16-18hr    39 embryo 18-20hr    31 embryo 20-22hr    47 embryo 22-24hr    42 larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    40 white prepupae new    42 white prepupae 12hr    40 white prepupae 24hr    48 pupae 2d postWPP    40 pupae 3d postWPP    28 pupae 4d postWPP    17 adult male 01day    27 adult male 05day    36 adult male 30day    28 adult female 01day    24 adult female 05day    41 adult female 30day    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    125 embryo 04-06hr    100 embryo 06-08hr    91 embryo 08-10hr    93 embryo 10-12hr    110 embryo 12-14hr    66 embryo 14-16hr    42 embryo 16-18hr    39 embryo 18-20hr    31 embryo 20-22hr    47 embryo 22-24hr    42 larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    40 white prepupae new    42 white prepupae 12hr    40 white prepupae 24hr    48 pupae 2d postWPP    40 pupae 3d postWPP    28 pupae 4d postWPP    17 adult male 01day    27 adult male 05day    36 adult male 30day    28 adult female 01day    24 adult female 05day    41 adult female 30day    45 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    125 embryo 04-06hr    100 embryo 06-08hr    91 embryo 08-10hr    93 embryo 10-12hr    110 embryo 12-14hr    66 embryo 14-16hr    42 embryo 16-18hr    39 embryo 18-20hr    31 embryo 20-22hr    47 embryo 22-24hr    42 larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    40 white prepupae new    42 white prepupae 12hr    40 white prepupae 24hr    48 pupae 2d postWPP    40 pupae 3d postWPP    28 pupae 4d postWPP    17 adult male 01day    27 adult male 05day    36 adult male 30day    28 adult female 01day    24 adult female 05day    41 adult female 30day    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (73) embryo 02-04hr  (125) embryo 04-06hr  (100) embryo 06-08hr  (91) embryo 08-10hr  (93) embryo 10-12hr  (110) embryo 12-14hr  (66) embryo 14-16hr  (42) embryo 16-18hr  (39) embryo 18-20hr  31 embryo 20-22hr  (47) embryo 22-24hr  (42) larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (40) white prepupae new  (42) white prepupae 12hr  (40) white prepupae 24hr  (48) pupae 2d postWPP  (40) pupae 3d postWPP  28 pupae 4d postWPP    17 adult male 01day  27 adult male 05day  (36) adult male 30day  28 adult female 01day    24 adult female 05day  (41) adult female 30day  (45) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr  125 embryo 04-06hr    100 embryo 06-08hr    91 embryo 08-10hr    93 embryo 10-12hr  110 embryo 12-14hr    66 embryo 14-16hr    42 embryo 16-18hr    39 embryo 18-20hr    31 embryo 20-22hr    47 embryo 22-24hr    42 larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    40 white prepupae new    42 white prepupae 12hr    40 white prepupae 24hr    48 pupae 2d postWPP    40 pupae 3d postWPP    28 pupae 4d postWPP    17 adult male 01day    27 adult male 05day    36 adult male 30day    28 adult female 01day    24 adult female 05day    41 adult female 30day    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    125 embryo 04-06hr    100 embryo 06-08hr    91 embryo 08-10hr    93 embryo 10-12hr    110 embryo 12-14hr    66 embryo 14-16hr    42 embryo 16-18hr    39 embryo 18-20hr    31 embryo 20-22hr    47 embryo 22-24hr    42 larva L1    20 larva L2    20 larva L3 12hr old    18 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    40 white prepupae new    42 white prepupae 12hr    40 white prepupae 24hr    48 pupae 2d postWPP    40 pupae 3d postWPP    28 pupae 4d postWPP    17 adult male 01day    27 adult male 05day    36 adult male 30day    28 adult female 01day    24 adult female 05day    41 adult female 30day    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    82 Schneider line 2 Sg4    63 embryonic 1182-4H    30 embryonic GM2    46 embryonic Kc167    45 embryonic S1    64 embryonic S3    54 leg disc CME L1    44 wing disc CME-W2    46 wing disc ML-DmD8    45 wing disc ML-DmD9    45 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    85 wing disc ML-DmD32    38 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    62 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    60 tumorous blood cells mbn2    53 ovary fGS/OSS    44 ovary OSC    38 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (82) Schneider line 2 Sg4  (63) embryonic 1182-4H  (30) embryonic GM2  (46) embryonic Kc167  (45) embryonic S1  (64) embryonic S3  (54) leg disc CME L1  (44) wing disc CME-W2  (46) wing disc ML-DmD8  (45) wing disc ML-DmD9  (45) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  (85) wing disc ML-DmD32  (38) haltere disc ML-DmD17-c3  (60) eye-antennal disc ML-DmD11  (46) antennal disc ML-DmD20-c5  (62) mixed discs ML-DmD4-c1  (45) CNS ML-DmBG1-c1  (43) CNS ML-DmBG2-c2  (60) tumorous blood cells mbn2  (53) ovary fGS/OSS  (44) ovary OSC  (38) ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    82 Schneider line 2 Sg4    63 embryonic 1182-4H    30 embryonic GM2    46 embryonic Kc167    45 embryonic S1    64 embryonic S3    54 leg disc CME L1    44 wing disc CME-W2    46 wing disc ML-DmD8    45 wing disc ML-DmD9    45 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    85 wing disc ML-DmD32    38 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    62 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    60 tumorous blood cells mbn2    53 ovary fGS/OSS    44 ovary OSC    38 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    82 Schneider line 2 Sg4    63 embryonic 1182-4H    30 embryonic GM2    46 embryonic Kc167    45 embryonic S1    64 embryonic S3    54 leg disc CME L1    44 wing disc CME-W2    46 wing disc ML-DmD8    45 wing disc ML-DmD9    45 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    85 wing disc ML-DmD32    38 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    62 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    60 tumorous blood cells mbn2    53 ovary fGS/OSS    44 ovary OSC    38 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    82 Schneider line 2 Sg4    63 embryonic 1182-4H    30 embryonic GM2    46 embryonic Kc167    45 embryonic S1    64 embryonic S3    54 leg disc CME L1    44 wing disc CME-W2    46 wing disc ML-DmD8    45 wing disc ML-DmD9    45 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    85 wing disc ML-DmD32    38 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    62 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    60 tumorous blood cells mbn2    53 ovary fGS/OSS    44 ovary OSC    38 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (82) Schneider line 2 Sg4  (63) embryonic 1182-4H  30 embryonic GM2  (46) embryonic Kc167  (45) embryonic S1  (64) embryonic S3  (54) leg disc CME L1  (44) wing disc CME-W2  (46) wing disc ML-DmD8  (45) wing disc ML-DmD9  (45) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  (85) wing disc ML-DmD32  (38) haltere disc ML-DmD17-c3  (60) eye-antennal disc ML-DmD11  (46) antennal disc ML-DmD20-c5  (62) mixed discs ML-DmD4-c1  (45) CNS ML-DmBG1-c1  (43) CNS ML-DmBG2-c2  (60) tumorous blood cells mbn2  (53) ovary fGS/OSS  (44) ovary OSC  (38) ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    82 Schneider line 2 Sg4    63 embryonic 1182-4H    30 embryonic GM2    46 embryonic Kc167    45 embryonic S1    64 embryonic S3    54 leg disc CME L1    44 wing disc CME-W2    46 wing disc ML-DmD8    45 wing disc ML-DmD9    45 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    85 wing disc ML-DmD32    38 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    62 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    60 tumorous blood cells mbn2    53 ovary fGS/OSS    44 ovary OSC    38 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    82 Schneider line 2 Sg4    63 embryonic 1182-4H    30 embryonic GM2    46 embryonic Kc167    45 embryonic S1    64 embryonic S3    54 leg disc CME L1    44 wing disc CME-W2    46 wing disc ML-DmD8    45 wing disc ML-DmD9    45 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    85 wing disc ML-DmD32    38 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    62 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    60 tumorous blood cells mbn2    53 ovary fGS/OSS    44 ovary OSC    38 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult  (32) Cadmium 50 mM 6 hrs, larvae L3  26 Cadmium 50 mM 12 hrs, larvae L3  (35) Cadmium 50 mM 48 hrs, 4-day adult  26 Cadmium 100 mM 48 hrs, 4-day adult  (37) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult  (30) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult  (33) Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult  32 Cadmium 50 mM 6 hrs, larvae L3  26 Cadmium 50 mM 12 hrs, larvae L3  (35) Cadmium 50 mM 48 hrs, 4-day adult  26 Cadmium 100 mM 48 hrs, 4-day adult  (37) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult  30 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult  (33) Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    13 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    24 Ethanol 10% 3 hrs, larvae L3    23 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • RE29729 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004915 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5193-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for TfIIB Stages(s) 1-3 Stages(s) 4-6 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of TfIIB Class Mutagen Stocks Known lesion TfIIBDG14311 P-element activity 2 --
Alleles Carried on Transgenic Constructs ( 36 )
For All Alleles Carried on Transgenic Constructs Show
Allele of TfIIB Class Mutagen Stocks Known lesion TfIIBKK102283 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TfIIB1-320.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes TfIIBAct5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TfIIBC33S.Act5C.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes TfIIBC33S.ΔC202.Act5C.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes TfIIBGD4061 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes TfIIBH17S.Act5C.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes TfIIBH17S.ΔC202.Act5C.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes TfIIBNIG.5193R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes TfIIBΔ121-157.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ121-157ΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ171-201.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ202-225.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ224-244.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ42-49.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ42-49.ΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ48-60.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ48-60ΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ48-98.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ48-98.ΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ63-74.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ63-88.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ63-88ΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ75-100.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ75-88.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ97-120.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔ97-120ΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔC170.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TfIIBΔC229.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔC278.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔC310.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔN121.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔN23.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔN23ΔC202.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TfIIBΔN47.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4061} NA P{KK102283} NA P{NIG.5193R} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{wHy}TfIIBDG14311 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
transcription factor binding inferred from physical interaction with Trf (Hansen et al., 1997)
Biological Process
CV term Evidence References
regulation of DNA binding inferred from direct assay (Hansen et al., 1997) regulation of transcription from RNA polymerase II promoter inferred from direct assay (Wampler and Kadonaga, 1992) transcriptional start site selection at RNA polymerase II promoter inferred from direct assay (Wampler and Kadonaga, 1992)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Wampler and Kadonaga, 1992)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
TBP-class protein binding inferred from electronic annotation with InterPro:IPR013150 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR000812, InterPro:IPR013137 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
regulation of transcription from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 1992.12.1) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity with human GTF2B AND inferred from sequence or structural similarity with Saccharomyces SUA7 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1992.12.1) transcription factor complex inferred from sequence or structural similarity with human GTF2B AND inferred from sequence or structural similarity with Saccharomyces SUA7 (Aoyagi and Wassarman, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
TfIIB - Trf2 pull down, western blot (Rabenstein et al., 1999)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions TfIIB allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60511 DroID - A comprehensive database of gene and protein interactions. • FBgn0004915 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34430 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P29052
Orthologs
OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6W6PG4 Dipteran inclusive ortholog search EOG6WWRTB Insect inclusive ortholog search EOG680GSC Arthropod inclusive ortholog search EOG63TXB7 Metazoa inclusive ortholog search EOG6CGC35
Orthologs in Drosophila Species (EOG6W6PG4)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\TfIIB     Drosophila simulans   Dsim\GD23702 Y   Drosophila sechellia   Dsec\GM18405 Y   Drosophila erecta   Dere\GG10133 Y   Drosophila yakuba   Dyak\GE18945 Y   Drosophila ananassae   Dana\GF15790 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA27746 Y   Drosophila persimilis   Dper\GL19146 Y   Drosophila willistoni   Dwil\GK14874 Y   Drosophila virilis   Dvir\TfIIB Y   Drosophila mojavensis   Dmoj\GI20422 Y   Drosophila grimshawi   Dgri\GH13607 Y
Orthologs in non-Drosophila Dipterans (EOG6WWRTB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005368   Anopheles gambiae Malaria mosquito  Agam\AGAP009558   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006843
Orthologs in non-Dipteran Insects (EOG680GSC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\TfIIB   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006176   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17155   Atta cephalotes Leafcutter ant  Acep\ACEP12185   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15662   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21884   Linepithema humile Argentine ant  Lhum\LH14538   Pogonomyrmex barbatus Red harvester ant  Pbar\PB22589   Solenopsis invicta Red fire ant  Sinv\SINV23964   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000165   Bombyx mori Silkmoth  Bmor\BGIBMGA006014   Pediculus humanus Human body louse  Phum\PHUM626400   Tribolium castaneum Red flour beetle  Tcas\TC010900
Orthologs in non-Insect Arthropods (EOG63TXB7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_325996   Daphnia pulex Water flea  Dpux\COI   Ixodes scapularis Deer tick  Isca\ISCW023433
Orthologs in non-Arthropod Metazoa (EOG6CGC35)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ttb-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013844gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014172gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005661gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027839gn   Danio rerio Zebrafish  Drer\gtf2b   Xenopus tropicalis Western clawed frog  Xtro\gtf2b   Gallus gallus Domestic chicken  Ggal\GTF2B   Mus musculus House mouse  Mmus\Gtf2b   Rattus norvegicus Norway rat  Rnor\Gtf2b   Homo sapiens Human  Hsap\GTF2B
Human Orthologs (1)
Gene OMIM HGNC Hsap\GTF2B
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23702 Drosophila sechellia Dsec\GM18405 Drosophila erecta Dere\GG10133 Drosophila yakuba Dyak\GE18945 Drosophila ananassae Dana\GF15790 Drosophila pseudoobscura pseudoobscura Dpse\GA27746 Drosophila persimilis Dper\GL19146 Drosophila willistoni Dwil\GK14874 Drosophila virilis Dvir\TfIIB Drosophila mojavensis Dmoj\GI20422 Drosophila grimshawi Dgri\GH13607
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	20415 y1 w67c23; P{wHy}TfIIBDG14311/SM6a
Kyoto	114592 yd2 w1118 P{ey-FLP.N}2; P{wHy}TfIIBDG14311 P{neoFRT}40A/CyO y+
VDRC	v106688 P{KK102283}VIE-260B
Genomic Clones ( 2 )
	BACR34C10 BACR48K04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 100 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD24035 RE29729
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT12067 AT16663 AT16961 AT28716 GM08210 LD05596 LD06694 LD14147 LD20868 LD48080 RE57533 RE71816 RH70589 SD01254
Other clones	12087 38796 53856 55105 60528 75168 75389 81491 105169 105196 148082 148852 164467 174254 183087 193874 273590 280855 288060 CK01.18D.H5 CK01.50A.D2 CK01.85D.D8 CK01.90C.E5 CK01.91A.D5 CK01.96C.E10 CK01.108C.F5 CK01.112B.G12 CK00063 Dmel_neb_016034_0943_0460 EC12704 EK062934 EK067848 EK070419 EK071315 EK097923 EK113019 EK136007 EK174913 EK178128 EK182859 EK186614 EK205344 EK206010 EK215030 EK230238 EK230537 EK230701 EK253763 EK255052 EK305812 EN03054 ESG014a.E21_B11 FGM303D12 RP003011529 RP003014318 RP003042988 RP003053492 RP003071942 RP003108911 RP003166688 RP003168950 RP003194213 RP003221089 RP003256750 RP003259639 RP003261415 RP003330555 RP003344712 RP003353960 RP003392334 RP003404704 RP003456371 RP003469378 RP003524038 RP003527274 RP003531224 RP003542116 RP003558722 RP003563639 RP003566689 RP003568505 RP003610894 RP003640781 RP003654496
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004915 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34430
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Antibodies are used to locate the TfIIB protein to polytene chromosomes. (Morris et al., 1997) Tbp and TfIIB genes are sequenced and characterised revealing common features in the promoter regions. (Lira-De Vito et al., 1995) Kr monomer (the activator) interacts with TfIIB, Kr dimers do not (FBrf0082559). Kr dimers engage in protein-protein interaction with TfIIEβ (FBrf0082559). Kr monomer-dimer transition alters Krs ability to interact with the general transcription machinery (FBrf0082559). (Roberts and Green, 1995) Activity of the glutamine rich ftz activation domain is blocked by truncated TfIIB derivatives in Schneider L2 cells suggesting the block is mediated by interactions with TfIIB. (Colgan et al., 1993) Isolated from a cDNA library using the human TFIIB gene as a probe. (Wampler and Kadonaga, 1992) TfIIB has been isolated, sequenced and characterised in vitro. (Wampler and Kadonaga, 1992) TfIIB has been cloned and sequenced. (Yamashita et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057540 NM_001273420 RefSeq (Proteins) • NP_476888 NP_001260349 DDBJ / EMBL / GenBank • AAA28929 AAA28930 AAA68626 AAA79093 AAF52951 AAT94498 AC007451 AGB92884 BI234090 BT015269 M88164 M91081 U02879 U35148 Entrez Gene - A searchable database of RefSeq genes. • 34430 UniProtKB/Swiss-Prot • P29052
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor TFIIB (IPR000812) Zinc finger, TFIIB-type (IPR013137) Transcription factor TFIIB, cyclin-like domain (IPR013150) Cyclin-like (IPR013763) Transcription factor TFIIB, conserved site (IPR023486) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE29729
Linkouts
	BioGRID - A database of protein and genetic interactions • 60511 DroID - A comprehensive database of gene and protein interactions. • FBgn0004915 DRSC - Results from RNAi screens. • FBgn0004915 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004915 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5193-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004915 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34430 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34430 modMine - Data generated by the modENCODE project. • FBgn0004915 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P29052
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG5193 (Aoyagi and Wassarman, 2000, Ardehali et al., 2009, Keleman et al., 2009.8.5) dmTFIIB (Lebedeva, 2005, Lebedeva et al., 2005) dTFIIB (Li and Manley, 1999, Li and Manley, 1998, Morris et al., 1997, Frank et al., 1995) dTfIIB (Wampler and Kadonaga, 1992) TfIIB (Turner et al., 2008, Andersen et al., 2010, Japanese National Institute of Genetics, 2012.5.21) TFIIB (Lebedeva, 2005, Lebedeva et al., 2005, Ma, 2005, Fu et al., 2004, Arnosti, 2002, Gu et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001, Park et al., 2001, Baek, 1995.8.30, Pham et al., 1999, Pazin et al., 1998, Sandaltzopoulos and Becker, 1998, Mason and Lis, 1997, Hansen et al., 1997, Gupta et al., 1996, ten Harmsel and Biggin, 1995, Austin and Biggin, 1995, George et al., 1995, Colgan et al., 1995, Lira-De Vito et al., 1995, Wampler et al., 1990, Kim and Lis, 2005, Fu and Ma, 2005, Ardehali et al., 2009, Hsu et al., 2008, Santoso and Kadonaga, 2006, Lebedeva et al., 2007, Tokusumi et al., 2012, Lam et al., 2012) Tfiib (Joshi and Nguyen, 1995) TfllB (Pruteanu-Malinici et al., 2011)
Name Synonym	Transcription factor IIB
Secondary FlyBase IDs
	FBgn0023206
References ( 77 )
Generate a list of	All references relating to this gene ( 77 )
List References by type	Research paper  ( 47 ) Supplementary material  ( 6 ) Review  ( 5 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 3 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Lam et al., 2012, PLoS Genet. 8(6): e1002736 The NSL Complex Regulates Housekeeping Genes in Drosophila. [FBrf0218697] Tokusumi et al., 2012, PLoS ONE 7(7): e41604 Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland. [FBrf0219204] Pruteanu-Malinici et al., 2011, PLoS Comput. Biol. 7(7): e1002098 Automatic Annotation of Spatial Expression Patterns via Sparse Bayesian Factor Models. [FBrf0214618] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...