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General Information
Symbol
Dmel\phm
Species
D. melanogaster
Name
phantom
Annotation Symbol
CG6578
Feature Type
FlyBase ID
FBgn0004959
Gene Model Status
Stock Availability
Gene Snapshot
phantom (phm) encodes a cytochrome P450 involved in ecdysteroid biosynthesis. It is an endoplasmic reticulum protein that catalyzes the addition of a hydroxyl group to the 25 carbon of the cholesterol side chain. phm mutants fail to undergo head involution, dorsal closure and cuticle secretion. [Date last reviewed: 2019-03-14]
Also Known As

Cyp306a1, exo, Cyp306a1-Dm

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:18,685,766..18,688,433 [+]
Recombination map

1-60

RefSeq locus
NC_004354 REGION:18685766..18688433
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000670347
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN001210788
(assigned by GO_Central )
Biological Process (8 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000670347
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000670347
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000670347
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000670347
(assigned by GO_Central )
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000670347
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000670347
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the cytochrome P450 family. (Q9VWR5)
Summaries
Gene Group (FlyBase)
HALLOWEEN CYTOCHROMES P450 -
The Halloween genes are members of the cytochrome P450 superfamily of enzymes involved the ecdysteroid biosynthetic pathway identified from embryonic lethal mutants. (Adapted from FBrf0204727 and FBrf0201926).
Protein Function (UniProtKB)
Involved in the metabolism of insect hormones; responsible for ecdysteroid C25-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
(UniProt, Q9VWR5)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
exo: exocephalon
Sex-linked recessive lethal; variably differentiated cuticle; complete failure of head involution with well developed head structures on exterior of the embryo. Many embryos slightly twisted with defective ventral denticle patterns.
phm: phantom
Male lethal. Cuticle of lethal embryos not well differentiated and contracted posteriorly.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\phm for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0074603
1997
574
FBtr0342846
2203
574
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0074374
65.2
574
6.11
FBpp0309669
65.2
574
6.11
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

574 aa isoforms: phm-PA, phm-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\phm using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
dorsal ectoderm anlage

Comment: anlage in statu nascendi

mesoderm anlage

Comment: anlage in statu nascendi

ventral ectoderm anlage

Comment: anlage in statu nascendi

antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\phm in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of phm
Transgenic constructs containing regulatory region of phm
Deletions and Duplications ( 11 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (21)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
2 of 15
Yes
No
2 of 15
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No
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No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (51)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
Yes
3 of 15
Yes
No
2 of 15
No
No
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No
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No
Rattus norvegicus (Norway rat) (44)
2 of 13
Yes
No
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No
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No
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No
Xenopus tropicalis (Western clawed frog) (13)
1 of 12
Yes
No
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No
1 of 12
Yes
No
1 of 12
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No
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No
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No
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No
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No
1 of 12
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Yes
1 of 12
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No
1 of 12
Yes
Yes
Danio rerio (Zebrafish) (49)
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
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No
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Yes
No
Caenorhabditis elegans (Nematode, roundworm) (40)
4 of 15
Yes
Yes
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
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Arabidopsis thaliana (thale-cress) (149)
3 of 9
Yes
No
3 of 9
Yes
No
2 of 9
No
No
2 of 9
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No
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