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Gene Dmel\U2af50

General Information
Symbol	Dmel\U2af50	Species	D. melanogaster
Name	U2 small nuclear riboprotein auxiliary factor 50	Annotation symbol	CG9998
Feature type	protein_coding_gene	FlyBase ID	FBgn0005411
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	U2AF, dU2AF50, l(1)9-21, dU2AF50, l(1)14Ca, l(1)14Cc
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	14B17-14B18	Sequence location	X:16,250,729..16,253,072 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene U2 small nuclear riboprotein auxiliary factor 50 is referred to in FlyBase by the symbol Dmel\U2af50 (CG9998, FBgn0005411). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: poly-pyrimidine tract binding; protein binding; RNA binding. There is experimental evidence that it is involved in the biological process: nuclear mRNA splicing, via spliceosome; nuclear export; mitotic spindle organization; RNA splicing; regulation of alternative nuclear mRNA splicing, via spliceosome; neurogenesis. 33 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain; U2 snRNP auxilliary factor, large subunit, splicing factor. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval trachea, adult female reproductive system, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult testis, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1633213_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of U2af50. Gene sequence location is X:16250729..16253072.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation. Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	Sequence features HFA20297 BKN22886
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 14B17-14B18   Limits computationally determined from genome sequence between P{EP}Cyp1EP1073&P{EP}Dsp1EP355 and P{EP}EP1493EP1493
Experimentally Determined Cytological Location
Cytogenetic map Notes References 14C1-14C2     (Kanaar, 1993.12.6)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\U2af50 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) U2af50-RA FBtr0074234  NM_057543   1527   416 U2af50-RB FBtr0307176     1344   427 U2af50-RC FBtr0307177     1371   360 U2af50-RD FBtr0307178     1417   416
Additional Transcript Data & Comments
Reported size (kB)	1.6 (northern blot) (Kanaar et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein U2af50-PA   FBpp0074013   46.7   416   7.56     NP_476891   AAF48596 U2af50-PB   FBpp0298005   48.1   427   7.25       U2af50-PC   FBpp0298006   39.5   360   4.89       U2af50-PD   FBpp0298007   46.7   416   7.56
Additional Polypeptide Data & Comments
Reported size (kDa)	416 (aa); 47 (kD predicted) (Kanaar et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) U2 snRNP auxilliary factor, large subunit, splicing factor (IPR006529) Nucleotide-binding, alpha-beta plait (IPR012677)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142048   AA142049   AA201787   AA202269   AA246335   AA247007   AA264361   AA439178   AA538909   AA539311   AA694970   AA696265   AA735570   AA735637   AA802460   AA803533   AA942449   AA942536   AA949918   AE014298 AAF48596   AC010039   AC010846   AI063129   AI108636   AI257675   AI405043   AI455206   AI456595   AI456709   AI456777   AI512032   AI516128   AI520091   AI533649   AW941532   AY069320 AAL39465   BF491268   BF492448   BF494884   BF494893   BF496059   BF496075   BF496827   BF503033   BF506073   BG634780   BI163905   BI164563   BI170015   BI170200   BI172909   BI212143   BI212167   BI213920   BI214960   BI215183   BI216493   BI229635   BI229874   BI230601   BI230912   BI232413   BI236424   BI237092   BI237098   BI238456   BI239676   BI240510   BI362179   BI362188   BI362722   BI362765   BI362866   BI362977   BI366097   BI366956   BI368758   BI368998   BI370707   BI372823   BI372857   BI374008   BI375703   BI375778   BI375860   BI482202   BI482559   BI482676   BI483325   BI483332   BI483707   BI485077   BI485079   BI485419   BI486329   BI486907   BI571980   BI572000   BI577645   BI577798   BI579181   BI579963   BI580352   BI580568   BI589106   BI611040   BI612690   BI615774   BI618229   BI619251   BI623354   BI633267   BI640388   BP546187   CA803556   CK605144   CK605145   CK605257   CK605258   CO302115   CO302469   CO303190   CO311629   CO316545   CO317472   CO323798   DQ443413   DQ443414   EC074265   EC077983   EC089542   EC195570   EC202305   EC209043   EC223292   EC223716   EC235088   EC248925   EV579801   EV580302   EV587707   GH758087   GH784831   GH789986   GH790528   GH804577   GH814690   GH821360   GH843838   GH846125   GH853851   GH854945   GH860388   GH868019   GH877594   GH887270   GH903985   GH905840   GH909816   GH913107   GH916757   GH922497   GH927969   GH937757   L23404
UniProtKB/Swiss-Prot	Q24562
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Kanaar et al., 1993)
Additional Descriptive Data
	U2af50 transcripts are abundant in RNA from 0-12hr embryos and Kc cells and are present throughout development. (Kanaar et al., 1993)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0005411    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0005411 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0005411 expression level Developmental Stage   Expression Level embryo 00-02hr    9767 embryo 02-04hr    17088 embryo 04-06hr    19311 embryo 06-08hr    14792 embryo 08-10hr    14130 embryo 10-12hr    15107 embryo 12-14hr    7818 embryo 14-16hr    6057 embryo 16-18hr    4812 embryo 18-20hr    3890 embryo 20-22hr    3865 embryo 22-24hr    3918 larva L1    3418 larva L2    2928 larva L3 12hr old    2280 larva L3 puffstage 1-2    2912 larva L3 puffstage 3-6    3550 larva L3 puffstage 7-9    4147 white prepupae new    4118 white prepupae 12hr    4976 white prepupae 24hr    6137 pupae 2d postWPP    4677 pupae 3d postWPP    2769 pupae 4d postWPP    2212 adult male 01day    2400 adult male 05day    2734 adult male 30day    2570 adult female 01day    3679 adult female 05day    5290 adult female 30day    5565 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9767) embryo 02-04hr  (17088) embryo 04-06hr  (19311) embryo 06-08hr  (14792) embryo 08-10hr  (14130) embryo 10-12hr  (15107) embryo 12-14hr  (7818) embryo 14-16hr  (6057) embryo 16-18hr  (4812) embryo 18-20hr  (3890) embryo 20-22hr  (3865) embryo 22-24hr  (3918) larva L1  (3418) larva L2  (2928) larva L3 12hr old  (2280) larva L3 puffstage 1-2  (2912) larva L3 puffstage 3-6  (3550) larva L3 puffstage 7-9  (4147) white prepupae new  (4118) white prepupae 12hr  (4976) white prepupae 24hr  (6137) pupae 2d postWPP  (4677) pupae 3d postWPP  (2769) pupae 4d postWPP  (2212) adult male 01day  (2400) adult male 05day  (2734) adult male 30day  (2570) adult female 01day  (3679) adult female 05day  (5290) adult female 30day  (5565) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9767) embryo 02-04hr  (17088) embryo 04-06hr  (19311) embryo 06-08hr  (14792) embryo 08-10hr  (14130) embryo 10-12hr  (15107) embryo 12-14hr  (7818) embryo 14-16hr  (6057) embryo 16-18hr  (4812) embryo 18-20hr  (3890) embryo 20-22hr  (3865) embryo 22-24hr  (3918) larva L1  (3418) larva L2  (2928) larva L3 12hr old  (2280) larva L3 puffstage 1-2  (2912) larva L3 puffstage 3-6  (3550) larva L3 puffstage 7-9  (4147) white prepupae new  (4118) white prepupae 12hr  (4976) white prepupae 24hr  (6137) pupae 2d postWPP  (4677) pupae 3d postWPP  (2769) pupae 4d postWPP  (2212) adult male 01day  (2400) adult male 05day  (2734) adult male 30day  (2570) adult female 01day  (3679) adult female 05day  (5290) adult female 30day  (5565) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9767 embryo 02-04hr  (17088) embryo 04-06hr  (19311) embryo 06-08hr  (14792) embryo 08-10hr  (14130) embryo 10-12hr  (15107) embryo 12-14hr    7818 embryo 14-16hr    6057 embryo 16-18hr    4812 embryo 18-20hr    3890 embryo 20-22hr    3865 embryo 22-24hr    3918 larva L1    3418 larva L2    2928 larva L3 12hr old    2280 larva L3 puffstage 1-2    2912 larva L3 puffstage 3-6    3550 larva L3 puffstage 7-9    4147 white prepupae new    4118 white prepupae 12hr    4976 white prepupae 24hr    6137 pupae 2d postWPP    4677 pupae 3d postWPP    2769 pupae 4d postWPP    2212 adult male 01day    2400 adult male 05day    2734 adult male 30day    2570 adult female 01day    3679 adult female 05day    5290 adult female 30day    5565 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9767 embryo 02-04hr    17088 embryo 04-06hr    19311 embryo 06-08hr    14792 embryo 08-10hr    14130 embryo 10-12hr    15107 embryo 12-14hr    7818 embryo 14-16hr    6057 embryo 16-18hr    4812 embryo 18-20hr    3890 embryo 20-22hr    3865 embryo 22-24hr    3918 larva L1    3418 larva L2    2928 larva L3 12hr old    2280 larva L3 puffstage 1-2    2912 larva L3 puffstage 3-6    3550 larva L3 puffstage 7-9    4147 white prepupae new    4118 white prepupae 12hr    4976 white prepupae 24hr    6137 pupae 2d postWPP    4677 pupae 3d postWPP    2769 pupae 4d postWPP    2212 adult male 01day    2400 adult male 05day    2734 adult male 30day    2570 adult female 01day    3679 adult female 05day    5290 adult female 30day    5565 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0005411 expression level Developmental Stage   Expression Level embryo 00-02hr    9767 embryo 02-04hr    17088 embryo 04-06hr    19311 embryo 06-08hr    14792 embryo 08-10hr    14130 embryo 10-12hr    15107 embryo 12-14hr    7818 embryo 14-16hr    6057 embryo 16-18hr    4812 embryo 18-20hr    3890 embryo 20-22hr    3865 embryo 22-24hr    3918 larva L1    3418 larva L2    2928 larva L3 12hr old    2280 larva L3 puffstage 1-2    2912 larva L3 puffstage 3-6    3550 larva L3 puffstage 7-9    4147 white prepupae new    4118 white prepupae 12hr    4976 white prepupae 24hr    6137 pupae 2d postWPP    4677 pupae 3d postWPP    2769 pupae 4d postWPP    2212 adult male 01day    2400 adult male 05day    2734 adult male 30day    2570 adult female 01day    3679 adult female 05day    5290 adult female 30day    5565 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9767) embryo 02-04hr  (17088) embryo 04-06hr  (19311) embryo 06-08hr  (14792) embryo 08-10hr  (14130) embryo 10-12hr  (15107) embryo 12-14hr  (7818) embryo 14-16hr  (6057) embryo 16-18hr  (4812) embryo 18-20hr  (3890) embryo 20-22hr  (3865) embryo 22-24hr  (3918) larva L1  (3418) larva L2  (2928) larva L3 12hr old  (2280) larva L3 puffstage 1-2  (2912) larva L3 puffstage 3-6  (3550) larva L3 puffstage 7-9  (4147) white prepupae new  (4118) white prepupae 12hr  (4976) white prepupae 24hr  (6137) pupae 2d postWPP  (4677) pupae 3d postWPP  (2769) pupae 4d postWPP  (2212) adult male 01day  (2400) adult male 05day  (2734) adult male 30day  (2570) adult female 01day  (3679) adult female 05day  (5290) adult female 30day  (5565) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9767) embryo 02-04hr  (17088) embryo 04-06hr  (19311) embryo 06-08hr  (14792) embryo 08-10hr  (14130) embryo 10-12hr  (15107) embryo 12-14hr  (7818) embryo 14-16hr  (6057) embryo 16-18hr  (4812) embryo 18-20hr  (3890) embryo 20-22hr  (3865) embryo 22-24hr  (3918) larva L1  (3418) larva L2  (2928) larva L3 12hr old  2280 larva L3 puffstage 1-2  (2912) larva L3 puffstage 3-6  (3550) larva L3 puffstage 7-9  (4147) white prepupae new  (4118) white prepupae 12hr  (4976) white prepupae 24hr  (6137) pupae 2d postWPP  (4677) pupae 3d postWPP  (2769) pupae 4d postWPP  2212 adult male 01day  2400 adult male 05day  (2734) adult male 30day  (2570) adult female 01day  (3679) adult female 05day  (5290) adult female 30day  (5565) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9767 embryo 02-04hr  17088 embryo 04-06hr  19311 embryo 06-08hr  14792 embryo 08-10hr  14130 embryo 10-12hr  15107 embryo 12-14hr    7818 embryo 14-16hr    6057 embryo 16-18hr    4812 embryo 18-20hr    3890 embryo 20-22hr    3865 embryo 22-24hr    3918 larva L1    3418 larva L2    2928 larva L3 12hr old    2280 larva L3 puffstage 1-2    2912 larva L3 puffstage 3-6    3550 larva L3 puffstage 7-9    4147 white prepupae new    4118 white prepupae 12hr    4976 white prepupae 24hr    6137 pupae 2d postWPP    4677 pupae 3d postWPP    2769 pupae 4d postWPP    2212 adult male 01day    2400 adult male 05day    2734 adult male 30day    2570 adult female 01day    3679 adult female 05day    5290 adult female 30day    5565 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9767 embryo 02-04hr    17088 embryo 04-06hr    19311 embryo 06-08hr    14792 embryo 08-10hr    14130 embryo 10-12hr    15107 embryo 12-14hr    7818 embryo 14-16hr    6057 embryo 16-18hr    4812 embryo 18-20hr    3890 embryo 20-22hr    3865 embryo 22-24hr    3918 larva L1    3418 larva L2    2928 larva L3 12hr old    2280 larva L3 puffstage 1-2    2912 larva L3 puffstage 3-6    3550 larva L3 puffstage 7-9    4147 white prepupae new    4118 white prepupae 12hr    4976 white prepupae 24hr    6137 pupae 2d postWPP    4677 pupae 3d postWPP    2769 pupae 4d postWPP    2212 adult male 01day    2400 adult male 05day    2734 adult male 30day    2570 adult female 01day    3679 adult female 05day    5290 adult female 30day    5565 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0005411    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0005411 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval trachea, adult female reproductive system, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult testis, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0005411 expression level Tissue   Expression Level Larval Central Nervous System    747.425 Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea    507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop    525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca    580.8 Adult InseminatedFemale Spermatheca    532.9 Adult Ovary    633.9 Adult Testis    332.5 Adult Male Accessory Gland    566.1 Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (747.425) Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea  507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop  525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca  (580.8) Adult InseminatedFemale Spermatheca  532.9 Adult Ovary  (633.9) Adult Testis    332.5 Adult Male Accessory Gland  (566.1) Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    747.425 Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea    507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop    525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca    580.8 Adult InseminatedFemale Spermatheca    532.9 Adult Ovary    633.9 Adult Testis    332.5 Adult Male Accessory Gland    566.1 Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    747.425 Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea    507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop    525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca    580.8 Adult InseminatedFemale Spermatheca    532.9 Adult Ovary    633.9 Adult Testis    332.5 Adult Male Accessory Gland    566.1 Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0005411 expression level Tissue   Expression Level Larval Central Nervous System    747.425 Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea    507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop    525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca    580.8 Adult InseminatedFemale Spermatheca    532.9 Adult Ovary    633.9 Adult Testis    332.5 Adult Male Accessory Gland    566.1 Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  747.425 Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea  507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop  525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca  580.8 Adult InseminatedFemale Spermatheca  532.9 Adult Ovary  633.9 Adult Testis    332.5 Adult Male Accessory Gland  566.1 Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    747.425 Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea    507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop    525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca    580.8 Adult InseminatedFemale Spermatheca    532.9 Adult Ovary    633.9 Adult Testis    332.5 Adult Male Accessory Gland    566.1 Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    747.425 Larval Midgut    284.8 Larval Hindgut    351.3 Larval Malpighian Tubules    408.9 Larval Fat Body    381.6 Larval Salivary Gland    465.3 Larval Trachea    507.55 Larval Carcass    234.425 Adult Head    308.6 Adult Eye    414.125 Adult Brain    407.2 Adult Thoracic-Abdominal Ganglion    367.5 Adult Crop    525.5 Adult Midgut    301.3 Adult Hindgut    462.8 Adult Malpighian Tubules    397.6 Adult Fat Body    298 Adult Salivary Gland    413.3 Adult Heart    274.775 Adult VirginFemale Spermatheca    580.8 Adult InseminatedFemale Spermatheca    532.9 Adult Ovary    633.9 Adult Testis    332.5 Adult Male Accessory Gland    566.1 Adult Carcass    311.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    308.6   NA  Eye    414.125   NA  Brain    407.2   747.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    367.5   NA  Crop    525.5   284.8  Midgut    301.3   351.3  Hindgut    462.8   408.9  Malpighian Tubules    397.6   381.6  Fat Body    298   465.3  Salivary Gland    413.3   NA  Heart    274.775   507.55  Trachea    NA   NA  VirginFemale Spermatheca    580.8   NA  InseminatedFemale Spermatheca    532.9   NA  Ovary    633.9   NA  Testis    332.5   NA  Male Accessory Gland    566.1   234.425  Carcass    311.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005411 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9998-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 U2af50GD13809 lethal | heat sensitive U2af50XR15 lethal | larval stage | recessive U2af504a3-1 U2af509-21 U2af50XR15 U2af50XR18 lethal | pupal stage, with Scer\GAL4pnr-MD237 U2af50GD13809 lethal | recessive U2af509-21 U2af50XR15 U2af50XR26 Other Phenotypes Allele neuroanatomy defective | larval stage, with Scer\GAL41407 U2af50GD13809 wild-type U2af50+t10 U2af50+tB12 U2af50+tS12 U2af50RS.7.final U2af50RS.7 U2af50T:Ivir\HA1 U2af50ΔRS.final U2af50ΔRS.true U2af50ΔRS Phenotype manifest in Allele embryonic/larval neuroblast | larval stage, with Scer\GAL41407 U2af50GD13809 ganglion mother cell | larval stage, with Scer\GAL41407 U2af50GD13809
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of U2af50 Class Mutagen Stocks Known lesion U2af50GG01531 1 -- U2af504a3-1 X ray 0 -- U2af506b4 X ray 0 -- U2af509-21 spontaneous 0 -- U2af50CPTI002092 piggyBac transposase 0 Yes U2af50XR15 X ray 0 -- U2af50XR18 X ray 0 -- U2af50XR26 X ray 0 --
Alleles Carried on Transgenic Constructs ( 25 )
For All Alleles Carried on Transgenic Constructs Show
Allele of U2af50 Class Mutagen Stocks Known lesion U2af50GD13809 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes U2af50JF02693 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes U2af50KK102790 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes U2af50+mBa in vitro construct - minigene 0 Yes U2af50+t10 in vitro construct - genomic fragment 0 Yes U2af50+tB12 in vitro construct - genomic fragment 0 Yes U2af50+tS12 in vitro construct - genomic fragment 0 Yes U2af50D150A.E151A in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes U2af50D204N.ts in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes U2af50hs.P in vitro construct - regulatory fusion 0 Yes U2af50mut.1.T:Ivir\HA1 in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes U2af50mut.2.T:Ivir\HA1 in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes U2af50mut.3.T:Ivir\HA1 in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes U2af50mut.4.T:Ivir\HA1 in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes U2af50RS.7.extreme in vitro construct 0 Yes U2af50RS.7.final in vitro construct 0 Yes U2af50RS.7 in vitro construct 0 Yes U2af50S284Y.ts in vitro construct - site directed mutagenesis 0 Yes U2af50T160I in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes U2af50T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - genomic fragment 0 Yes U2af50ΔBst in vitro construct 0 Yes U2af50ΔRS.extreme in vitro construct - deletion 0 Yes U2af50ΔRS.final in vitro construct - deletion 0 Yes U2af50ΔRS.true in vitro construct - deletion 0 Yes U2af50ΔRS in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)4b18 (Stanewsky et al., 1993) Df(1)6b4 (Stanewsky et al., 1993) Df(1)l32 (Lindsley, 1993, Banga et al., 1995) Df(1)r75c (Jones and Rubin, 1990) Df(1)XR26 (Rudner et al., 1998, Stanewsky et al., 1993)
Duplicated in	Dp(1;2)r+75c (Schalet, 1986, Banga et al., 1995) Dp(1;4)80f18c (Banga et al., 1995) Dp(1;4)81l8g (Banga et al., 1995) Dp(1;4)81l12h (Banga et al., 1995)
Not disrupted in	Df(1)r-D1 (Schalet, 1986, Lindsley, 1993, Banga et al., 1995) T(1;4)9e2-10 (Stanewsky et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13809} NA P{KK102790} NA P{TRiP.JF02693} NA heat-shock construct P{U2af50hs.P} NA characterization construct P{9-21/dU2AF50genomic} NA P{211S12} NA P{235B12} NA P{HA-U2af50.mut1} NA P{HA-U2af50.mut2} NA P{HA-U2af50.mut3} NA P{HA-U2af50.mut4} NA P{HA-U2af50} NA P{tra2::U2af50C.T:FLAG} NA P{tra2::U2af50C.T:lacZ} NA P{tra2::U2af50N.T:FLAG} NA P{U2af50.+mBa} NA P{U2af50.D150A.E151A} NA P{U2af50.D204N.ts} NA P{U2af50.S284Y.ts} NA P{U2af50.T160I} NA P{U2af50.ΔRS.extreme} NA P{U2af50.ΔRS.final} NA P{U2af50.ΔRS.true} NA P{U2af50.ΔRS} NA P{U2af50(RS)7.extreme} NA P{U2af50(RS)7.final} NA P{U2af50(RS)7} NA P{U2af50ΔBst} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{Mae-UAS.6.11}U2af50GG01531 NA miscellaneous insertions PBac{681.P.FSVS-1}U2af50CPTI002092 NA
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
poly-pyrimidine tract binding inferred from direct assay (Rudner et al., 1998) protein binding inferred from physical interaction with U2af38 (Taliaferro et al., 2011) RNA binding inferred from mutant phenotype (Taliaferro et al., 2011)
Biological Process
CV term Evidence References
mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) nuclear export inferred from mutant phenotype (Blanchette et al., 2004) nuclear mRNA splicing, via spliceosome inferred from mutant phenotype (Rudner et al., 1998) regulation of alternative nuclear mRNA splicing, via spliceosome inferred from mutant phenotype (Park et al., 2004) RNA splicing inferred from mutant phenotype (Taliaferro et al., 2011)
Cellular Component
CV term Evidence References
precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-) poly-pyrimidine tract binding non-traceable author statement (Swiss-Prot Project Members, 1998.12.15) protein heterodimerization activity non-traceable author statement (Swiss-Prot Project Members, 1998.12.15)
Biological Process
CV term Evidence References
nuclear mRNA splicing, via spliceosome inferred by curator from GO:0071011 (Herold et al., 2009) inferred from sequence or structural similarity with NCBI:NP_009210 AND inferred from sequence or structural similarity with Saccharomyces MUD2 (Mount and Salz, 2000) non-traceable author statement (Kanaar, 1993.12.6, Swiss-Prot Project Members, 1998.12.15) spliceosome assembly non-traceable author statement (Kanaar, 1993.12.6)
Cellular Component
CV term Evidence References
nucleus inferred by curator from GO:0000381 (Park et al., 2004) non-traceable author statement (Swiss-Prot Project Members, 1998.12.15) spliceosomal complex inferred from sequence or structural similarity with NCBI:NP_009210 AND inferred from sequence or structural similarity with Saccharomyces MUD2 (Mount and Salz, 2000) non-traceable author statement (Kanaar, 1993.12.6) U2 snRNP traceable author statement (Rudner et al., 1998)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions U2af50 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58942 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005411 DroID - A comprehensive database of gene and protein interactions. • FBgn0005411 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32602
Orthologs
Genome-wide drosophilid orthologs	Dana\GF19596 Dere\GG19328 Dgri\GH12033 Dmoj\GI14308 Dper\GL15893 Dpse\GA22177 Dsec\GM13406 Dsim\GD24494 Dvir\GJ19364 Dwil\GK10063 Dyak\GE15975
Curated drosophilid orthologs	Dpse\GA22177
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL002818-PA Aedes aegypti (mosquito) AGAP002908-PA Anopheles gambiae (mosquito) XP_623055.1 Apis mellifera (honey bee) XP_001951521.1 Acyrthosiphon pisum (pea aphid) At1g60900.1 Arabidopsis thaliana (wall cress) 21058 Batrachochytrium dendrobatidis (chytrid fungus) 249005 Branchiostoma floridae (lancelet) XP_001893425.1 Brugia malayi (roundworm nematode) BGIBMGA007082-PA Bombyx mori (silk worm) ENSBTAP00000003664 Bos taurus (bovine) CN26797 Caenorhabditis brenneri (small nematode) CBP18334 Caenorhabditis briggsae (nematode) CE27339 Caenorhabditis elegans (worm) ENSCAFP00000003724 Canis familiaris (dog) Chro.10345 Cryptosporidium hominis (obligate parasite) ENSCINP00000007092 Ciona intestinalis (sea squirt) JA14936 Caenorhabditis japonica (nematode) cn06349 Cryptococcus neoformans (yeast-like fungus) cgd1_3080 Cryptosporidium parvum (protozoan parasite) CPIJ012850 Culex pipens (mosquito) ENSCPOP00000001429 Cavia porcellus (guinea pig) 111628 Chlamidomonas reinhardtii (green alga) RP04366 Caenorhabditis remanei (nematode) ENSCSAVP00000011610 Ciona savignyi (sedentary tunicate) 155651 Capitella spl (polychaete worm) DDB0233353 Dictyostelium discoideum (amoeba) 232197 Daphnia pulex (daphnia) ENSDARP00000002857 Danio rerio (zebra fish) ENSECAP00000008276 Equus caballus (horse) XP_653619 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000014064 Gymnopilus aculeatus (mushroom) 185543 Hypsipyla robusta (cedar shoot borer) ENSP00000307863 Homo sapiens (human) ISCW002946-PA Ixodes scapularis (deer tick) 190527 Lottia gigantea (owl limpet) LmjF03.0520 Leishmania major (virus) 1352 Monocosta brevicollis (choanoflagellate) ENSMMUP00000006397 Macaca mulatta (rhesus monkey) ENSMUSP00000005041 Mus musculus (house mouse) 228171 Nematostella vectensis (starlet anemone) XP_001604333.1 Nasonia vitripennis (wasp) ENSORLP00000014464 Oryzias latipes (japanese killifish) LOC_Os11g45590.1 Oryza sativa (asian rice) 16445 Ostreococcus tauri (green alga) PF07_0066 Plasmodium falciparum (protozoal parasite) PHUM002336-PA Pediculus humanus (human body louse) PP27287 Pristionchus pacificus (nematode) 130002 Physcomitrella patens (moss) ENSPPYP00000011687 Pongo pygmaeus (orangutan) 76050 Phytophthora ramorum (sudden oak death pathogen) 134114 Phytophthora sojae (root rot pathogen) 424261 Populus trichocarpa (black cottonwood) ENSPTRP00000019813 Pan troglodytes (chimpanzee) ENSRNOP00000048275 Rattus norvegicus (norway rat) 4982776 Sorghum bicolor (sorghum) Smp_019690 Schistosoma mansoni (trematode parasite) SPBC146.07 Schizosaccharomyces pombe (fission yeast) XP_792919.2 Strongylocentrotus purpuratus (purple sea urchin) 21652 Trichoplax adhaerens (metazoa) TA17245 Theileria annulata (apicomplexan parasite) XP_974465.1 Tribolium castaneum (red flour beetle) Tc00.1047053510265.40 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053510311.50 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000014196 Tetraodon nigroviridis (green spotted pufferfish) TP04_0035 Theileria parva (parasitic protozoan) 263000 Thalassiosira pseudonana (marine diatom) ENSTRUP00000016891 Takifugu rubripes (pufferfish Takifugu) 3722.m00010 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000041471 Xenopus tropicalis (frog) YALI0E09889g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11909 LH14548 ISCW002946 FBgn0233531 FBgn0212078 FBgn0206507 FBgn0195829 FBgn0168337 JGI_V11_232197 FBgn0082156 FBgn0153497 FBgn0137061 FBgn0119512 FBgn0111532 FBgn0096603 CPIJ012850 CPIJ011203 BGIBMGA007082 ACYPI010039 ACYPI006614 ACYPI001129 GB12876 AGAP002908 AAEL002818 PB22679 PHUM460480 GLEAN_05736
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	27542 y1 v1; P{TRiP.JF02693}attP2 14619 y1 w67c23 P{Mae-UAS.6.11}U2af50GG01531
VDRC	v107723 P{KK102790}VIE-260B v24176 w1118; P{GD13809}v24176 v24177 w1118; P{GD13809}v24177
Genomic Clones ( 2 )
	BACR13G13 BACR47D09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 153 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD03714
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03652 AT03664 AT08446 AT09768 AT09788 AT10819 AT18931 AT27969 AT29390 AT31116 GH02626 GH07842 GH24951 GM01869 GM05401 GM05592 GM13419 LD02525 LD05178 LD06109 LD08049 LD13665 LD18565 LD19005 LD21540 LD21657 LD21744 LD26678 LD26807 LD28482 LD36663 LD36823 LD36904 LD40052 LD41885 LD43658 LP06105 RE03433 RE04263 RE11350 RE11580 RE14314 RE15611 RE16178 RE17167 RE17192 RE19567 RE21128 RE21523 RE23639 RE27457 RE27748 RE28475 RE32477 RE33252 RE33260 RE34928 RE36239 RE37362 RE46674 RE46686 RE47369 RE47420 RE47543 RE47676 RE51551 RE52611 RE54786 RE55070 RE57430 RE60006 RE60046 RE61509 RE63567 RE63651 RE63755 RE64860 RE65263 RE65393 RE66201 RE66209 RE66631 RE68350 RE68352 RE68738 RE69748 RE70390 RE71356 RE71545 RE73169 RE74073 RE74535 RE74779 RH07311 RH07335 RH17131 RH30785 RH41631 RH45529 RH48829 RH50245 RH55492 SD05548 SD23227 SD27312
Other clones	425 58911 59720 63611 65101 102516 120680 161501 163759 CK00184 Dmel_dig_018111_0651_3110 Dmel_dig_018643_0581_3072 Dmel_neb_007395_0170_3668 EK183152 EK184224 EK191520 EK225615 EK240463 EK265233 EK268314 ESG011c.E21_D01 FGH103G04 RP003026359 RP003052308 RP003061026 RP003069843 RP003110850 RP003137175 RP003154810 RP003163627 RP003206517 RP003236626 RP003239278 RP003251588 RP003280076 RP003324995 RP003358888 RP003388532 RP003421060 RP003483411 RP003486853 RP003527940 RP003559133 RP003578585 RP003622965 UT01712 UT01792
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0005411 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32602
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in a long metaphase spindle with misaligned chromosomes when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) Nuclear export of large intronless mRNA. (Blanchette et al., 2004) Studies in vivo reveal that the RS domain of U2af50 is completely dispensable: the domain is not required for U2 snRNP recruitment during spliceosome assembly in vivo. (Rudner et al., 1998) E.coli copurification assay is used to map the domain on U2af50 that interacts with U2af38: a 28 amino acid fragment is necessary and sufficient for the interaction. Interaction studies in vivo indicate that the U2af50/U2af38 heterodimer is essential for viability in vivo. (Rudner et al., 1998) The presence of one RS domain of U2AF, on either the large subunit (encoded by U2af50) or the small subunit (encoded by U2af38), promotes high-affinity pyrimidine tract RNA binding activity. (Rudner et al., 1998) Germline transformation of a wild type copy of U2af50 is capable of rescuing a lethal mutation, establishing that U2af50 is essential for viability. (Kanaar et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057543 RefSeq (Proteins) • NP_476891 DDBJ / EMBL / GenBank • AA201787 AAA03548 AAF48596 AAL39465 AC010039 AC010846 AW941532 AY069320 L23404 Entrez Gene - A searchable database of RefSeq genes. • 32602 UniProtKB/Swiss-Prot • Q24562
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) U2 snRNP auxilliary factor, large subunit, splicing factor (IPR006529) Nucleotide-binding, alpha-beta plait (IPR012677)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58942 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005411 DroID - A comprehensive database of gene and protein interactions. • FBgn0005411 DRSC - Results from RNAi screens. • FBgn0005411 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005411 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9998-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0005411 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32602 InParanoid A subset of ortholog calls from InParanoid. • AAEL002818-PA Aedes aegypti (mosquito) AGAP002908-PA Anopheles gambiae (mosquito) XP_623055.1 Apis mellifera (honey bee) XP_001951521.1 Acyrthosiphon pisum (pea aphid) At1g60900.1 Arabidopsis thaliana (wall cress) 21058 Batrachochytrium dendrobatidis (chytrid fungus) 249005 Branchiostoma floridae (lancelet) XP_001893425.1 Brugia malayi (roundworm nematode) BGIBMGA007082-PA Bombyx mori (silk worm) ENSBTAP00000003664 Bos taurus (bovine) CN26797 Caenorhabditis brenneri (small nematode) CBP18334 Caenorhabditis briggsae (nematode) CE27339 Caenorhabditis elegans (worm) ENSCAFP00000003724 Canis familiaris (dog) Chro.10345 Cryptosporidium hominis (obligate parasite) ENSCINP00000007092 Ciona intestinalis (sea squirt) JA14936 Caenorhabditis japonica (nematode) cn06349 Cryptococcus neoformans (yeast-like fungus) cgd1_3080 Cryptosporidium parvum (protozoan parasite) CPIJ012850 Culex pipens (mosquito) ENSCPOP00000001429 Cavia porcellus (guinea pig) 111628 Chlamidomonas reinhardtii (green alga) RP04366 Caenorhabditis remanei (nematode) ENSCSAVP00000011610 Ciona savignyi (sedentary tunicate) 155651 Capitella spl (polychaete worm) DDB0233353 Dictyostelium discoideum (amoeba) 232197 Daphnia pulex (daphnia) ENSDARP00000002857 Danio rerio (zebra fish) ENSECAP00000008276 Equus caballus (horse) XP_653619 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000014064 Gymnopilus aculeatus (mushroom) 185543 Hypsipyla robusta (cedar shoot borer) ENSP00000307863 Homo sapiens (human) ISCW002946-PA Ixodes scapularis (deer tick) 190527 Lottia gigantea (owl limpet) LmjF03.0520 Leishmania major (virus) 1352 Monocosta brevicollis (choanoflagellate) ENSMMUP00000006397 Macaca mulatta (rhesus monkey) ENSMUSP00000005041 Mus musculus (house mouse) 228171 Nematostella vectensis (starlet anemone) XP_001604333.1 Nasonia vitripennis (wasp) ENSORLP00000014464 Oryzias latipes (japanese killifish) LOC_Os11g45590.1 Oryza sativa (asian rice) 16445 Ostreococcus tauri (green alga) PF07_0066 Plasmodium falciparum (protozoal parasite) PHUM002336-PA Pediculus humanus (human body louse) PP27287 Pristionchus pacificus (nematode) 130002 Physcomitrella patens (moss) ENSPPYP00000011687 Pongo pygmaeus (orangutan) 76050 Phytophthora ramorum (sudden oak death pathogen) 134114 Phytophthora sojae (root rot pathogen) 424261 Populus trichocarpa (black cottonwood) ENSPTRP00000019813 Pan troglodytes (chimpanzee) ENSRNOP00000048275 Rattus norvegicus (norway rat) 4982776 Sorghum bicolor (sorghum) Smp_019690 Schistosoma mansoni (trematode parasite) SPBC146.07 Schizosaccharomyces pombe (fission yeast) XP_792919.2 Strongylocentrotus purpuratus (purple sea urchin) 21652 Trichoplax adhaerens (metazoa) TA17245 Theileria annulata (apicomplexan parasite) XP_974465.1 Tribolium castaneum (red flour beetle) Tc00.1047053510265.40 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053510311.50 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000014196 Tetraodon nigroviridis (green spotted pufferfish) TP04_0035 Theileria parva (parasitic protozoan) 263000 Thalassiosira pseudonana (marine diatom) ENSTRUP00000016891 Takifugu rubripes (pufferfish Takifugu) 3722.m00010 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000041471 Xenopus tropicalis (frog) YALI0E09889g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/u2af1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32602 modMine - Data generated by the modENCODE project. • FBgn0005411 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11909 LH14548 ISCW002946 FBgn0233531 FBgn0212078 FBgn0206507 FBgn0195829 FBgn0168337 JGI_V11_232197 FBgn0082156 FBgn0153497 FBgn0137061 FBgn0119512 FBgn0111532 FBgn0096603 CPIJ012850 CPIJ011203 BGIBMGA007082 ACYPI010039 ACYPI006614 ACYPI001129 GB12876 AGAP002908 AAEL002818 PB22679 PHUM460480 GLEAN_05736
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	9-21 (Jones and Rubin, 1990) CG9998 (Mount and Salz, 2000, Goshima et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Herold et al., 2009, McIntyre et al., 2006, Taliaferro et al., 2011) DmU2AF50 (Mazroui et al., 1999) dU2AF50 (Graveley and Celotto, 2003, Tacke and Manley, 1999) DU2AF50 (Page-McCaw et al., 1999) dU2AF50 (Hieronymus and Silver, 2004, Dauwalder and Mattox, 1998, Rudner et al., 1998, Rudner et al., 1998, Kennedy and Berget, 1997, Kanaar et al., 1993, Taliaferro et al., 2011) dUTAf50 (Blanchette et al., 2004) l(1)9-21 (Banga, 1996.5.2, Banga et al., 1995, Lindsley, 1993, Stanewsky et al., 1993, ) l(1)14Ca (Banga, 1996.5.2, Banga et al., 1995) l(1)14Cc (Lindsley, 1993, Stanewsky et al., 1993, ) U2AF (Smith and Valcarcel, 2000, Nagengast et al., 2004, Nagengast and Salz, 2004, Maniatis and Tasic, 2002, Graveley, 2002, Kennedy et al., 1998, Rudner et al., 1998) U2af (Rio, 2002) U2AF50 (Park et al., 2004, Kanaar, 1993.12.6, Penn et al., 2008) U2af50 (Legube et al., 2006, Blanco et al., 2010, Goshima et al., 2007, Lecuyer et al., 2007, Somma et al., 2008, Herold et al., 2009, McIntyre et al., 2006, Neumüller et al., 2011) U2AF-50 (Boucher et al., 2001)
Name Synonym	U2 small nuclear riboprotein auxiliary factor 50
Secondary FlyBase IDs
References ( 63 )
Generate a list of	All references relating to this gene ( 63 )
List References by type	Research paper  ( 32 ) Supplementary material  ( 5 ) Review  ( 6 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 3 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 3 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Taliaferro et al., 2011, Genes Dev. 25(6): 608--620 Evolution of a tissue-specific splicing network. [FBrf0213285] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...