dU2AF50, U2AF, l(1)9-21, dU2AF50, l(1)14Cc
splicing factor required for the ATP-dependent association of U2 snRNP with pre-mRNA branchpoints - forms a heterodimer with the small splice factor U2af38 - U2af50 interacts with the intronic 3' polypyrimidine tract - the small subunit functions in recognition of the 3' AG dinucleotide
Gene model reviewed during 5.50
Gene model reviewed during 5.55
1.6 (northern blot)
416 (aa); 47 (kD predicted)
Forms a heterodimer with the U2AF small subunit.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\U2af50 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\U2af50 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in a long metaphase spindle with misaligned chromosomes when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Nuclear export of large intronless mRNA.
Studies in vivo reveal that the RS domain of U2af50 is completely dispensable: the domain is not required for U2 snRNP recruitment during spliceosome assembly in vivo.
E.coli copurification assay is used to map the domain on U2af50 that interacts with U2af38 : a 28 amino acid fragment is necessary and sufficient for the interaction. Interaction studies in vivo indicate that the U2af50/U2af38 heterodimer is essential for viability in vivo.