FB2020_06, released Dec 22, 2020

Gene: Dmel\Mbs

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General Information
Symbol
Dmel\Mbs
Species
D. melanogaster
Name
Myosin binding subunit
Annotation Symbol
CG32156
Feature Type
protein_coding_gene
FlyBase ID
FBgn0005536
Gene Model Status
Current
Stock Availability
22 publicly available
Gene Snapshot
Myosin binding subunit (Mbs) encodes a regulatory subunit of myosin phosphatase. It interacts with the catalytic subunit of the myosin phosphatase encoded by flw. It is involved in cell movement in various biological processes and in the arrest of constriction of contractile rings and ring canals during oogenesis. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

l(3)72Dd, DMBS, DMYPT, Myosin phosphatase, l(3)03802

Key Links
Genomic Location
Cytogenetic map
72D1-72D1
Sequence location
3L:16,051,959..16,082,026 [+]
Recombination map

3-44

RefSeq locus
NT_037436 REGION:16051959..16082026
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables enzyme inhibitor activity
inferred from biological aspect of ancestor with PANTHER:PTN001193544
(assigned by GO_Central )
(Gaudet et al., 2011)
enables phosphatase regulator activity
inferred from biological aspect of ancestor with PANTHER:PTN001193544
(assigned by GO_Central )
(Gaudet et al., 2011)
enables protein kinase binding
inferred from electronic annotation with InterPro:IPR031775
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (8 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
involved_in dorsal closure
inferred from mutant phenotype
(Tan et al., 2003, Mizuno et al., 2002)
involved_in epidermis development
inferred from mutant phenotype
(Ninov et al., 2007)
involved_in germline ring canal formation
inferred from mutant phenotype
(Ong et al., 2010)
involved_in imaginal disc morphogenesis
inferred from mutant phenotype
(Mitonaka et al., 2007)
involved_in imaginal disc-derived wing hair organization
inferred from genetic interaction with FLYBASE:dsh; FB:FBgn0000499
(Lee and Treisman, 2004)
involved_in neuron projection morphogenesis
inferred from mutant phenotype
(Petsakou et al., 2015)
involved_in regulation of compound eye photoreceptor development
inferred from mutant phenotype
(Lee and Treisman, 2004)
involved_in regulation of locomotor rhythm
inferred from mutant phenotype
(Petsakou et al., 2015)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
colocalizes_with apical cortex
inferred from direct assay
(Vasquez et al., 2014)
located_in apical cortex
inferred from direct assay
(Sen et al., 2012)
colocalizes_with apical junction complex
inferred from direct assay
(Mitonaka et al., 2007)
located_in apical part of cell
inferred from direct assay
(Sen et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN001193544
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Summary (Interactive Fly)

regulatory subunit of myosin phosphatase flapwing - involved in dorsal closure - involved in cell movement and in the arrest of constriction of contractile rings and ring canals during oogenesis.

Gene Model and Products
Number of Transcripts
18
Number of Unique Polypeptides
18

Please see the JBrowse view of Dmel\Mbs for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model includes transcripts encoding non-overlapping portions of the full CDS.

Gene model reviewed during 6.28

Sequence Ontology: Class of Gene
gene_with_edited_transcript
(Rodriguez et al., 2012)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mbs-RE
FBtr0075441
NM_140573
1906
377
Mbs-RF
FBtr0112850
NM_001104143
2404
367
Mbs-RG
FBtr0112851
NM_001104144
4062
795
Mbs-RH
FBtr0112852
NM_001104145
4445
925
Mbs-RI
FBtr0301472
NM_168646
5102
1144
Mbs-RJ
FBtr0301473
NM_168648
4955
1101
Mbs-RK
FBtr0301575
NM_168647
4257
860
Mbs-RL
FBtr0308212
NM_001259854
4778
1230
Mbs-RM
FBtr0308213
NM_001259855
5430
1243
Mbs-RN
FBtr0308214
NM_001259856
5686
1245
Mbs-RO
FBtr0308215
NM_001259857
5520
1273
Mbs-RP
FBtr0308216
NM_001259858
4003
341
Mbs-RQ
FBtr0333616
NM_001274990
4482
1111
Mbs-RR
FBtr0475266
NM_001382055
2869
522
Mbs-RS
FBtr0475267
NM_001382056
2371
532
Mbs-RT
FBtr0475268
NM_001382057
2782
493
Mbs-RU
FBtr0475269
NM_001382058
2317
338
Mbs-RV
FBtr0475270
NM_001382059
4395
1082
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Mbs-PE
FBpp0075199
40.3
377
10.55
NP_648830
AAF49546
Mbs-PF
FBpp0111763
39.3
367
10.35
NP_001097613
ABW08546
Mbs-PG
FBpp0111764
87.3
795
6.39
NP_001097614
ABW08547
Mbs-PH
FBpp0111765
101.2
925
7.05
NP_001097615
ABW08548
Mbs-PI
FBpp0290687
123.6
1144
5.54
NP_730099
AAN11758
Mbs-PJ
FBpp0290688
119.2
1101
5.41
NP_730101
AAN11759
Mbs-PK
FBpp0290790
93.7
860
5.16
NP_730100
AAF49547
Mbs-PL
FBpp0300532
133.0
1230
5.97
NP_001246783
AFH04454
Mbs-PM
FBpp0300533
133.8
1243
6.12
NP_001246784
AFH04455
Mbs-PN
FBpp0300534
134.0
1245
6.12
NP_001246785
AFH04456
Mbs-PO
FBpp0300535
137.4
1273
6.13
NP_001246786
AFH04457
Mbs-PP
FBpp0300536
37.8
341
5.62
NP_001246787
AFH04458
Mbs-PQ
FBpp0305792
120.2
1111
5.61
NP_001261919
AGB94612
Mbs-PR
FBpp0423332
55.3
522
10.27
QJC18622
Mbs-PS
FBpp0423333
56.3
532
10.43
QJC18623
Mbs-PT
FBpp0423334
51.8
493
10.31
QJC18624
Mbs-PU
FBpp0423335
35.7
338
10.40
QJC18625
Mbs-PV
FBpp0423336
116.7
1082
5.59
QJC18626
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mbs using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis & adult stage | female
female germline cyst | subset
(Yamamoto et al., 2013)
germarium | restricted
(Yamamoto et al., 2013)
Additional Descriptive Data

Mbs and flw are enriched in the female germ cells undergoing incomplete cytokinesis in a cyst stage-dependent manner, but not in the germ cells undergoing complete cytokinesis or those that have ceased dividing.

(Yamamoto et al., 2013)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with apical cortex
inferred from direct assay
(Vasquez et al., 2014)
located_in apical cortex
inferred from direct assay
(Sen et al., 2012)
colocalizes_with apical junction complex
inferred from direct assay
(Mitonaka et al., 2007)
located_in apical part of cell
inferred from direct assay
(Sen et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mbs in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal (with Df(3L)st-f13)
lethal (with Df(3L)th102)
lethal | germline clone | maternal effect
lethal | larval stage | recessive
lethal | pupal stage | recessive
lethal | recessive
lethal - all die before end of embryonic stage
lethal - all die before end of embryonic stage (with MbsT541)
lethal - all die before end of embryonic stage (with MbsT666)
lethal - all die before end of embryonic stage (with MbsT791)
lethal - all die before end of embryonic stage | maternal effect, with Mbs3/Df(3L)th117, Scer\GAL4- :
lethal - all die before end of embryonic stage | maternal effect (with Df(3L)th117), with MbsEP3727, Scer\GAL4- :
lethal - all die before end of larval stage (with Df(3L)th117)
lethal - all die before end of larval stage | recessive
lethal - all die before end of pupal stage, with Scer\GAL4da.G32
lethal - all die before end of pupal stage | recessive
viable, with Scer\GAL4- :
Sterility
Allele
female semi-sterile, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
female sterile
female sterile, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
fertile, with Mbs03802
fertile, with Mbshs.PT
Other Phenotypes
Allele
axis specification defective | embryonic stage 15, with Scer\GAL4how-24B
axis specification defective | embryonic stage 16, with Scer\GAL4how-24B
axis specification defective | embryonic stage 17, with Scer\GAL4how-24B
cell polarity defective | embryonic stage, with Scer\GAL4VP16.mat.αTub67C
cell polarity defective | recessive | somatic clone
cell shape defective | third instar larval stage (with Df(3L)Exel6127)
circadian behavior defective, with Scer\GAL4P0.5.Pdf
developmental rate defective (with Mbs3)
developmental rate defective (with MbsP2r31)
neuroanatomy defective, with Scer\GAL4P0.5.Pdf
neuroanatomy defective | somatic clone | third instar larval stage
some die during embryonic stage
some die during embryonic stage (with MbsT541)
some die during embryonic stage (with MbsT666)
some die during embryonic stage (with MbsT791)
some die during embryonic stage | maternal effect, with Mbs3/Df(3L)th117, Scer\GAL4- :
some die during embryonic stage | maternal effect (with Df(3L)th117), with MbsEP3727, Scer\GAL4- :
some die during larval stage (with Df(3L)th117)
some die during larval stage | recessive
some die during pupal stage, with Scer\GAL4da.G32
some die during pupal stage | recessive
visible, with Scer\GAL4- :
visible, with Scer\GAL4GMR.PF
Phenotype manifest in
Allele
adult abdomen, with Scer\GAL4NP0056
axon | somatic clone | third instar larval stage
centriole | germline clone
dorsal hair, with Mbs3/Df(3L)th117, Scer\GAL4- :
dorsal hair, with MbsEP3727/Df(3L)th117, Scer\GAL4- :
dorsal hair (with Df(3L)th117), with Mbs3, Scer\GAL4- :
dorsal hair (with Df(3L)th117), with MbsEP3727, Scer\GAL4- :
egg | germline clone
embryo | gastrula stage, with Scer\GAL4VP16.mat.αTub67C
embryo | maternal effect, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
embryonic/first instar larval cuticle
embryonic/first instar larval cuticle (with Df(3L)th102)
embryonic/first instar larval cuticle | dorsal, with Mbs3/Df(3L)th117, Scer\GAL4- :
embryonic/first instar larval cuticle | dorsal, with MbsEP3727/Df(3L)th117, Scer\GAL4- :
embryonic/first instar larval cuticle | dorsal (with Df(3L)th117), with Mbs3, Scer\GAL4- :
embryonic/first instar larval cuticle | dorsal (with Df(3L)th117), with MbsEP3727, Scer\GAL4- :
embryonic/larval anterior midgut | embryonic stage 15, with Scer\GAL4how-24B
embryonic/larval anterior midgut | embryonic stage 16, with Scer\GAL4how-24B
embryonic/larval anterior midgut | embryonic stage 17, with Scer\GAL4how-24B
embryonic/larval epidermis, with Scer\GAL4NP0056
embryonic leading edge cell
embryonic leading edge cell, with Mbs3/Df(3L)th117, Scer\GAL4- :
embryonic leading edge cell, with MbsEP3727/Df(3L)th117, Scer\GAL4- :
embryonic leading edge cell (with Df(3L)th117), with Mbs3, Scer\GAL4- :
embryonic leading edge cell (with Df(3L)th117), with MbsEP3727, Scer\GAL4- :
embryonic proventriculus | embryonic stage 15, with Scer\GAL4how-24B
embryonic proventriculus | embryonic stage 16, with Scer\GAL4how-24B
embryonic proventriculus | embryonic stage 17, with Scer\GAL4how-24B
eye, with Scer\GAL4GMR.PF
eye disc | somatic clone
germline cyst
imaginal disc | third instar larval stage (with Mbs3)
imaginal disc | third instar larval stage (with MbsP2r31)
larval neuroblast | third instar larval stage (with Df(3L)Exel6127)
morphogenetic furrow | somatic clone
nurse cell | germline clone
nurse cell ring canal | germline clone
oocyte | somatic clone
ovariole | germline clone
ring canal
ring canal, with Mbs03802
ring canal, with Mbshs.PT
ring canal (with Mbs03802)
ring canal (with MbsT541)
ring canal (with MbsT666)
ring canal | germline clone
s-LNv Pdf neuron, with Scer\GAL4P0.5.Pdf
wing, with Scer\GAL4- :
wing disc | somatic clone | third instar larval stage
wing disc | third instar larval stage (with Mbs3)
wing disc | third instar larval stage (with MbsP2r31)
wing hair | somatic clone
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PPP1R12B
13 of 15
Yes
Yes
1  
Hsap\PPP1R12A
10 of 15
No
Yes
1  
Hsap\PPP1R12C
5 of 15
No
Yes
Hsap\NRARP
1 of 15
No
Yes
Hsap\PPP1R16A
1 of 15
No
No
Hsap\PPP1R16B
1 of 15
No
No
Hsap\PPP1R27
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Ppp1r12a
11 of 15
Yes
Yes
Mmus\Ppp1r12b
11 of 15
Yes
Yes
Mmus\Ppp1r12c
5 of 15
No
Yes
Mmus\Nrarp
1 of 15
No
Yes
Mmus\Ppp1r16a
1 of 15
No
No
Mmus\Ppp1r16b
1 of 15
No
No
Mmus\Ppp1r27
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (8)
Rnor\Ppp1r12a
8 of 13
Yes
Yes
Rnor\Ppp1r12b
7 of 13
No
Yes
Rnor\Ppp1r12c
3 of 13
No
Yes
Rnor\Myo16
1 of 13
No
No
Rnor\Nrarp
1 of 13
No
Yes
Rnor\Ppp1r16a
1 of 13
No
No
Rnor\Ppp1r16b
1 of 13
No
No
Rnor\Ppp1r27
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (8)
Xtro\ppp1r12a
5 of 12
Yes
Yes
Xtro\ppp1r12c
5 of 12
Yes
Yes
Xtro\ppp1r12b
2 of 12
No
Yes
Xtro\LOC100487727
1 of 12
No
Yes
Xtro\nrarp
1 of 12
No
Yes
Xtro\ppp1r16a
1 of 12
No
No
Xtro\ppp1r16b
1 of 12
No
No
Xtro\ppp1r27
1 of 12
No
Yes
Danio rerio (Zebrafish) (9)
Drer\ppp1r12a
11 of 15
Yes
Yes
Drer\ppp1r12c
6 of 15
No
Yes
Drer\zmp:0000001167
4 of 15
No
Yes
Drer\LOC101885790
2 of 15
No
Yes
Drer\nrarpa
1 of 15
No
Yes
Drer\nrarpb
1 of 15
No
Yes
Drer\ppp1r16a
1 of 15
No
No
Drer\ppp1r16b
1 of 15
No
No
Drer\ppp1r27a
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\mel-11
11 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\AVO2
1 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919027L )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00011089
Drosophila simulans
Dsim\GD14592
Drosophila sechellia
Dsec\GM25580
Drosophila erecta
Dere\GG15946
Drosophila yakuba
Dyak\GE28499
Drosophila yakuba
Dyak\GE27692
Drosophila ananassae
Dana\GF23761
Drosophila pseudoobscura pseudoobscura
Dpse\GA16721
Drosophila persimilis
Dper\GL25084
Drosophila willistoni
Dwil\GK24127
Drosophila virilis
Dvir\GJ13261
Drosophila mojavensis
Dmoj\GI12323
Drosophila grimshawi
Dgri\GH16060
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501L9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17019927
Glossina morsitans
Tsetse fly
Gmor\GMOY004250
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_06737
Mayetiola destructor
Hessian fly
Mdes\Mdes001344
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012753
Anopheles gambiae
Malaria mosquito
Agam\GPROR49
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ006926
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01DO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA002008
Danaus plexippus
Monarch butterfly
Dple\DPOGS203899-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL013867
Apis florea
Little honeybee
Aflo\2521931892
Apis mellifera
Western honey bee
Amel\GB41075
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP16164
Bombus terrestris
Buff-tailed bumblebee
Bter\15209636
Linepithema humile
Argentine ant
Lhum\LH22983
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:003043
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG021010
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10269
Tribolium castaneum
Red flour beetle
Tcas\TC003209
Pediculus humanus
Human body louse
Phum\PHUM561400
Rhodnius prolixus
Kissing bug
Rpro\RPRC004776
Cimex lectularius
Bed bug
Clec\CLEC001169
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007693
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03203
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01BQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR011038
Ixodes scapularis
Black-legged tick
Isca\ISCW021906
Stegodyphus mimosarum
African social velvet spider
Smim\KFM78194.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur07g07080
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_234539
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02PF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_005112gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ppp1rs12B
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
MYPT-75D
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Mbs03802
    ameliorates  autosomal recessive nonsyndromic deafness 2
    modeled by ck07130, ck13
    (Todi et al., 2008)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PPP1R12B; protein phosphatase 1 regulatory subunit 12B
    13 of 15
    603768
         
        PPP1R12A; protein phosphatase 1 regulatory subunit 12A
        10 of 15
        602021
           
          PPP1R12C; protein phosphatase 1 regulatory subunit 12C
          5 of 15
          613245
              Functional Complementation Data
              Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
              Interactions
              Summary of Physical Interactions
              esyN Network Diagram
              Show neighbor-neighbor interactions:
              Select Layout:
              Legend:
              Protein
              RNA
              Selected Interactor(s)
              Interactions Browser
              View in Interactions Browser
              Please see the Physical Interaction reports below for full details
              protein-protein
              Physical Interaction
              Assay
              References
              Mbs - Dsor1
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Friedman et al., 2011)
              Mbs - Egfr
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Friedman et al., 2011)
              Mbs - Jheh3
              experimental knowledge based
              (Guruharsha et al., 2011)
              Mbs - Patj
              anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot, anti tag coimmunoprecipitation
              (Sen et al., 2012)
              Mbs - Pp1-87B
              anti tag coimmunoprecipitation, anti tag western blot
              (Vereshchagina et al., 2004)
              Mbs - baz
              anti tag coimmunoprecipitation, western blot
              (Sen et al., 2015)
              Mbs - crb
              anti tag coimmunoprecipitation, western blot
              (Sen et al., 2015)
              Mbs - csw
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Friedman et al., 2011)
              Mbs - dos
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Friedman et al., 2011)
              Mbs - flw
              anti tag coimmunoprecipitation, anti tag western blot
              (Vereshchagina et al., 2004)
              Mbs - par-1
              anti tag coimmunoprecipitation, western blot, enzymatic study
              (Majumder et al., 2012)
              Mbs - rho
              enzymatic study, autoradiography
              (Mizuno et al., 2002)
              Mbs - rictor
              anti tag coimmunoprecipitation, Identification by mass spectrometry
              (Vinayagam et al., 2016)
              Mbs - slmb
              experimental knowledge based
              (Guruharsha et al., 2011)
              Mbs - sturkopf
              experimental knowledge based
              (Guruharsha et al., 2011)
              Summary of Genetic Interactions
              esyN Network Diagram
              esyN Network Key:
              Suppression
              Enhancement
              View in Interactions Browser
              Please look at the allele data for full details of the genetic interactions
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              Mbs
              suppressible
              Rok
              (Lee and Treisman, 2004)
              Mbs
              suppressible
              sqh
              (Lee and Treisman, 2004)
              Mbs
              enhanceable
              sqh
              (Lee and Treisman, 2004)
              Mbs
              suppressible
              zip
              (Lee and Treisman, 2004, Mizuno et al., 2002)
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              Dad
              suppressible
              Mbs
              (Widmann and Dahmann, 2009)
              dsh
              suppressible
              Mbs
              (Lee and Treisman, 2004)
              eys|prom
              enhanceable
              Mbs
              (Nie et al., 2014)
              flw
              enhanceable
              Mbs
              (Yamamoto et al., 2013)
              flw
              suppressible
              Mbs
              (Vereshchagina et al., 2004)
              kay
              suppressible
              Mbs
              (Franciscovich et al., 2008)
              Patj
              suppressible
              Mbs
              (Sen et al., 2012)
              pbl
              enhanceable
              Mbs
              (Gregory et al., 2007)
              pbl
              suppressible
              Mbs
              (Gregory et al., 2007)
              Rac1
              enhanceable
              Mbs
              (Tan et al., 2003)
              Rho1
              suppressible
              Mbs
              (Petsakou et al., 2015)
              Rho1
              enhanceable
              Mbs
              (Tan et al., 2003)
              Rho1|zip
              suppressible
              Mbs
              (Mizuno et al., 2002)
              RhoGEF2|zip
              suppressible
              Mbs
              (Mizuno et al., 2002)
              Rok
              suppressible
              Mbs
              (Lee and Treisman, 2004)
              Rok|zip
              suppressible
              Mbs
              (Mizuno et al., 2002)
              zip
              suppressible
              Mbs
              (Mizuno et al., 2002)
              External Data
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Pathways
              Signaling Pathways (FlyBase)
              Metabolic Pathways
              External Data
              Genomic Location and Detailed Mapping Data
              Chromosome (arm)
              3L
              Recombination map

              3-44

              Cytogenetic map
              72D1-72D1
              Sequence location
              3L:16,051,959..16,082,026 [+]
              FlyBase Computed Cytological Location
              Cytogenetic map
              Evidence for location
              72D1-72D1
              Limits computationally determined from genome sequence between P{lacW}thj5C8&P{PZ}Mbs03802 and P{lacW}SsRβs1939
              Experimentally Determined Cytological Location
              Cytogenetic map
              Notes
              References
              72D1-72D2
              (determined by in situ hybridisation)
              (Spradling et al., 1999, Perrimon et al., 1996)
              72C-72C
              (determined by in situ hybridisation)
              (Szeged Stock Center, 1998-, Deak et al., 1997)
              72D1-72D2
              (BDGP Project Members, 1994-1999)
              Experimentally Determined Recombination Data
              Location

              3-44

              (FlyBase, 2016)

              3-38.2

              (Comeron et al., 2012)
              Left of (cM)
              Right of (cM)
              Notes
              Stocks and Reagents
              Stocks (22)
              Genomic Clones (29)
              cDNA Clones (105)
               

              Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

              cDNA clones, fully sequenced
              BDGP DGC clones
              Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               

              polyclonal

              (Ong et al., 2010, Mitonaka et al., 2007, Mizuno et al., 2002)
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of

              Source for identity of: Mbs l(3)72Dd

              (Mizuno et al., 2002)
              Source for database merge of

              Source for merge of: l(3)S057306b l(3)S005331b

              (Szeged Stock Center, 1998-)

              Source for merge of: l(3)S095304 Mbs l(3)S057306b

              (Lee and Treisman, 2004)

              Source for merge of: Mbs CG5891

              (FlyBase, 1996-)
              Additional comments

              Annotations CG5600, CG5891 merged as CG32156 in release 3 of the genome annotation.

              (FlyBase Genome Annotators, 2002-2003)

              Source for merge of Mbs CG5891 was sequence comparison ( date:020814 ).

              (FlyBase, 1996-)
              Other Comments

              The 'l(3)72Dd' (Mbs) complementation group comprises 11 EMS-induced mutant alleles.

              (Cooper and Kennison, 2011)

              Mbs is enriched in cells undergoing incomplete cytokinesis.

              (Ong et al., 2010)

              Photoreceptor neurons require Mbs to reduce myosin activity and thus prevent photoreceptors from dropping out of the eye disc epithelium and moving toward and through the optic stalk. Mbs reduces myosin activity by dephosphorylating sqh.

              (Lee and Treisman, 2004)

              Mutations in Mbs cause embryos to develop a dorsal-open phenotype.

              (Tan et al., 2002)

              The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. Mbs is required for germ cell viability or early oogenesis.

              (Perrimon et al., 1996)
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 127 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DPiM - Drosophila Protein interaction map
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              FlyMine - An integrated database for Drosophila genomics
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Synonyms and Secondary IDs (30)
              Reported As
              Symbol Synonym
              0573/06
              (Deak et al., 1997)
              0953/04
              (Deak et al., 1997)
              CG32156
              (Meadows, 2017.1.11, Chen et al., 2012, Rees et al., 2011, Ni et al., 2010.12.1, Zhang et al., 2010, Fujikawa et al., 2009, Keleman et al., 2009.8.5, Berger et al., 2008, Carrera et al., 2008, Laviolette et al., 2005)
              CG5600
              (Montana and Littleton, 2006)
              CG5891
              (Stathopoulos et al., 2002)
              DMBS
              (Ferreira et al., 2014, Todi et al., 2008, Mitonaka et al., 2007, Song et al., 2007, Vereshchagina et al., 2004, Jacinto et al., 2002, Mizuno et al., 2002)
              DMYPT
              (Yamamoto et al., 2013, Ong et al., 2010, Ong and Tan, 2008, Balasubramanian et al., 2004, Tan et al., 2003, Tan et al., 2002)
              DMbs
              (Todi et al., 2005)
              MBS
              (Qin et al., 2018, Flores-Benitez and Knust, 2016, Mason et al., 2016, Munjal et al., 2015, Sen et al., 2015, Vasquez et al., 2014, Yashiro et al., 2014, Piekny et al., 2005)
              Mbs
              (Feuillette et al., 2020, Hoshika et al., 2020, Pulido Companys et al., 2020, Letizia et al., 2019, Tetley et al., 2019, Zeledon et al., 2019, Sharma et al., 2018, Solis et al., 2017, Clandinin and Owens, 2016-, Duque and Gorfinkiel, 2016, Jurado et al., 2016, Valencia-Expósito et al., 2016, Petsakou et al., 2015, Ashwal-Fluss et al., 2014, Nie et al., 2014, Sopko et al., 2014, Kwon et al., 2013, Gurudatta et al., 2012, Paik et al., 2012, Rodriguez et al., 2012, Ruggiero et al., 2012, Abruzzi et al., 2011, Cooper and Kennison, 2011, Friedman et al., 2011, Rees et al., 2011, Sun et al., 2011, Okumura et al., 2010, Brody, 2009.03, Christensen et al., 2009.5.6, Fujikawa et al., 2009, Widmann and Dahmann, 2009, Berger et al., 2008, Carrera et al., 2008, Christensen et al., 2008.4.15, Franciscovich et al., 2008, Baker et al., 2007, Buszczak et al., 2007, Gregory et al., 2007, Kirchner et al., 2007, Ninov et al., 2007, Hickson et al., 2006, Muraro and Moffat, 2006, Laviolette et al., 2005)
              Mypt
              l(3)03802
              (Perrimon et al., 1996)
              l(3)72Dd
              (Cooper and Kennison, 2011, Mitonaka et al., 2007, Song et al., 2007, Lee and Treisman, 2004, Spradling et al., 1999, Kennison, 1995.6.21, BDGP Project Members, 1994-1999)
              l(3)S005331b
              l(3)S057306
              l(3)S057306b
              l(3)S095304
              l(3)j3B4
              l(3)j7A1
              mbs
              (Pinheiro et al., 2017, Tsankova et al., 2017, Sen et al., 2012, Widmann and Dahmann, 2009, Corrigall et al., 2007)
              Name Synonyms
              MLC-phosphatase
              (Petit et al., 2002)
              Myosin binding subunit
              (Duque and Gorfinkiel, 2016, Sen et al., 2015, Mizuno et al., 2002)
              Myosin phosphatase
              (Martin, 2020, Tan et al., 2002)
              Myosin phosphatase-binding subunit
              (Corrigall et al., 2007)
              lethal (3) S057306b
              lethal (3) S095304
              lethal(3)72Dd
              myosin binding subunit
              (Foronda et al., 2012, Ruggiero et al., 2012, Shalaby et al., 2009, Song et al., 2007)
              myosin phosphatase target subunit
              (Ong et al., 2010)
              myosin-binding subunit
              (Mason et al., 2016)
              Secondary FlyBase IDs
              • FBgn0010817
              • FBgn0011346
              • FBgn0011399
              • FBgn0028052
              • FBgn0028127
              • FBgn0036561
              • FBgn0036562
              • FBgn0052156
              • FBgn0061116
              • FBgn0062264
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (145)