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Gene Dmel\Rab3

General Information
Symbol	Dmel\Rab3	Species	D. melanogaster
Name	Rab3	Annotation symbol	CG7576
Feature type	protein_coding_gene	FlyBase ID	FBgn0005586
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	DRab3, dm-Rab3, DmRab3
Genomic Location
Chromosome (arm)	2R	Recombination map	2-43--48
Cytogenetic map	47B7-47B7	Sequence location	2R:6,608,840..6,616,708 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Rab3 is referred to in FlyBase by the symbol Dmel\Rab3 (CG7576, FBgn0005586). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: GTPase activity. There is experimental evidence that it is involved in the biological process: maintenance of presynaptic active zone structure. 14 alleles are reported. The phenotypes of these alleles are annotated with: t-bar; neuromuscular junction. It has one annotated transcript and one annotated polypeptide. Protein features are: Small GTP-binding protein domain; Small GTPase superfamily; Small GTPase superfamily, Rab type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye. Comments on Affy2 ProbeSet: ProbeSet 1625838_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rab3. Gene sequence location is 2R:6608840..6616708.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 47B7-47B7   Limits computationally determined from genome sequence between P{PZ}lola00349&P{EP}lolaEP952 and P{lacW}l(2)k00909k00909&P{EP}EP2619EP2619
Experimentally Determined Cytological Location
Cytogenetic map Notes References 47B-47B   (determined by in situ hybridisation)   (Johnston et al., 1991) 47B-47B   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location	2-43--48 (Graf et al., 2009)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rab3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rab3-RA FBtr0088257  NM_078963   3757   220
Additional Transcript Data & Comments
Reported size (kB)	3.8 (northern blot) (DiAntonio et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rab3-PA   FBpp0087352   24.9   220   4.79     NP_523687   AAF58762
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Small GTPase superfamily (IPR001806) Small GTPase superfamily, Rab type (IPR003579) Small GTP-binding protein domain (IPR005225) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA391702   AA567466   AE013599 AAF58762   AB112932 BAD07037   AI107269   AI135638   AI257478   AI516938   AW944055   AY060449 AAL25488   BI166223   BI167411   BI168209   BI168560   BI214961   BI215490   BI238724   BI356298   BI357368   BI372665   BI481683   BI616438   BI626028   CK659252   CO188866   CO265652   CO265999   CO268980   CO273646   CO279927   CO280122   CO281177   CO282310   CO283413   CO296495   CO296705   CO310577   CO313920   CO316778   CO317365   CO317482   CO318976   CO319564   CO320390   CO322434   CO323732   CZ469537   EC052726   EC052751   EC053220   EC053961   EC054722   EC056113   EC063065   EC065352   EC066513   EC071141   EC073474   EC075869   EC078467   EC080742   EC083721   EC088221   EC201770   EC211248   EC212019   EC213071   EC214121   EC215810   EC215811   EC219961   EC221348   EC222136   EC227382   EC232524   EC233364   EC233576   EC238646   EC244138   EC248006   GH747893   GH747971   GH748331   GH751719   GH751865   GH753393   GH755342   GH755803   GH756286   GH756804   GH758605   GH759647   GH760988   GH772513   GH773332   GH776215   GH776483   GH777725   GH778659   GH780373   GH780690   GH782282   GH783440   GH786110   GH787703   GH788470   GH788532   GH789715   GH790474   GH790519   GH793039   GH797896   GH799259   GH800210   GH800546   GH800820   GH803487   GH805280   GH806158   GH806334   GH810326   GH811860   GH813428   GH814762   GH815724   GH817876   GH822748   GH823294   GH825351   GH825458   GH825735   GH826191   GH826727   GH826739   GH826765   GH827551   GH829781   GH829906   GH830149   GH830536   GH832487   GH834959   GH835617   GH836851   GH837145   GH837395   GH837513   GH837701   GH837872   GH838927   GH839275   GH841087   GH841838   GH842874   GH844070   GH844443   GH844626   GH844809   GH845180   GH846178   GH846343   GH847381   GH847917   GH848096   GH848221   GH848307   GH849498   GH849849   GH850076   GH851023   GH851114   GH851866   GH852241   GH855152   GH856472   GH856774   GH857784   GH858809   GH859769   GH859986   GH860979   GH864266   GH865039   GH865232   GH866648   GH868002   GH868207   GH871221   GH871749   GH875620   GH878317   GH878710   GH878827   GH881069   GH881114   GH881362   GH882821   GH884917   GH884932   GH886477   GH888085   GH888594   GH889867   GH893232   GH894126   GH894198   GH894540   GH895632   GH897090   GH897199   GH900023   GH900051   GH903355   GH905461   GH905628   GH907402   GH908196   GH908700   GH911327   GH914255   GH914257   GH914411   GH914669   GH915435   GH915733   GH916216   GH916409   GH916867   GH916990   GH917652   GH918594   GH919665   GH920622   GH921325   GH921611   GH922844   GH922853   GH923041   GH924125   GH924377   GH925055   GH925978   GH926437   GH926991   GH927173   GH927554   GH928112   GH929723   GH931206   GH931715   GH932058   GH932402   GH932873   GH932991   GH933666   GH935662   GH936522   GH936941   GH937425   GH938978   GH939088   GH939373   GH939937   GH940105   GH941021   GH941098   M64621 AAA28843   Z50312
UniProtKB/Swiss-Prot	P25228
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13 -- 16   dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic antennal sense organ   (Fisher et al., 2012) embryonic brain   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic labial sensory complex   (Fisher et al., 2012) embryonic maxillary sensory complex   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012) ventral midline   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (DiAntonio et al., 1993) embryonic stage 13 -- 17   presumptive embryonic/larval central nervous system   (DiAntonio et al., 1993) antenno-maxillary complex   (DiAntonio et al., 1993) adult stage   cell body of adult brain   (DiAntonio et al., 1993)
Additional Descriptive Data
	Rab3 transcripts are first detected in 12-15hr embryos on northern blots and are detected at all subsequent stages tested. The are enriched in adult head relative to adult body. Transcripts are detected by in situ hybridization in mid-late embryos in the CNS and in the antenno-maxillary complex. In sections of adult brain, transcripts are detected in the cell bodies of the central nuclei but not in the cortex of the visual system. (DiAntonio et al., 1993)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   synapse   • presynaptic active zone (Graf et al., 2009)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
presynaptic active zone inferred from direct assay (Graf et al., 2009) colocalizes_with presynaptic active zone inferred from direct assay (Petersen and Stowers, 2011) synapse inferred from direct assay (Chan et al., 2011) synaptic vesicle inferred from direct assay (Chan et al., 2011) colocalizes_with synaptic vesicle inferred from direct assay (Petersen and Stowers, 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1043.075 Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head    510.7 Adult Eye    403.95 Adult Brain    1540.9 Adult Thoracic-Abdominal Ganglion    1287.6 Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1043.075) Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head  510.7 Adult Eye    403.95 Adult Brain  (1540.9) Adult Thoracic-Abdominal Ganglion  (1287.6) Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1043.075 Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head    510.7 Adult Eye    403.95 Adult Brain  (1540.9) Adult Thoracic-Abdominal Ganglion  (1287.6) Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1043.075 Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head    510.7 Adult Eye    403.95 Adult Brain    1540.9 Adult Thoracic-Abdominal Ganglion    1287.6 Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1043.075 Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head    510.7 Adult Eye    403.95 Adult Brain    1540.9 Adult Thoracic-Abdominal Ganglion    1287.6 Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1043.075) Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head  510.7 Adult Eye    403.95 Adult Brain  (1540.9) Adult Thoracic-Abdominal Ganglion  (1287.6) Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1043.075 Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head    510.7 Adult Eye    403.95 Adult Brain  1540.9 Adult Thoracic-Abdominal Ganglion  1287.6 Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1043.075 Larval Midgut    12.6 Larval Hindgut    6.5 Larval Malpighian Tubules    1.3 Larval Fat Body    5.2 Larval Salivary Gland    5.2 Larval Trachea    14.125 Larval Carcass    9.575 Adult Head    510.7 Adult Eye    403.95 Adult Brain    1540.9 Adult Thoracic-Abdominal Ganglion    1287.6 Adult Crop    4.6 Adult Midgut    14.2 Adult Hindgut    6.7 Adult Malpighian Tubules    4.4 Adult Fat Body    12.1 Adult Salivary Gland    7.3 Adult Heart    8.3 Adult VirginFemale Spermatheca    9.6 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    1.2 Adult Testis    1.9 Adult Male Accessory Gland    3.4 Adult Carcass    27.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    510.7   NA  Eye    403.95   NA  Brain    1540.9   1043.075  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1287.6   NA  Crop    4.6   12.6  Midgut    14.2   6.5  Hindgut    6.7   1.3  Malpighian Tubules    4.4   5.2  Fat Body    12.1   5.2  Salivary Gland    7.3   NA  Heart    8.3   14.125  Trachea    NA   NA  VirginFemale Spermatheca    9.6   NA  InseminatedFemale Spermatheca    5.7   NA  Ovary    1.2   NA  Testis    1.9   NA  Male Accessory Gland    3.4   9.575  Carcass    27.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    73 central nervous system, pupae P8    220 head, virgin 1-day female    138 head, virgin 4-day female    120 head, virgin 20-day female    59 head, mated 1-day female    141 head, mated 4-day female    93 head, mated 20-day female    52 head, mated 1-day male    222 head, mated 4-day male    194 head, mated 20-day male    161 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3  (73) central nervous system, pupae P8  (220) head, virgin 1-day female  (138) head, virgin 4-day female  (120) head, virgin 20-day female  (59) head, mated 1-day female  (141) head, mated 4-day female  (93) head, mated 20-day female  (52) head, mated 1-day male  (222) head, mated 4-day male  (194) head, mated 20-day male  (161) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    73 central nervous system, pupae P8  (220) head, virgin 1-day female  (138) head, virgin 4-day female  (120) head, virgin 20-day female    59 head, mated 1-day female  (141) head, mated 4-day female    93 head, mated 20-day female    52 head, mated 1-day male  (222) head, mated 4-day male  (194) head, mated 20-day male  (161) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    73 central nervous system, pupae P8    220 head, virgin 1-day female    138 head, virgin 4-day female    120 head, virgin 20-day female    59 head, mated 1-day female    141 head, mated 4-day female    93 head, mated 20-day female    52 head, mated 1-day male    222 head, mated 4-day male    194 head, mated 20-day male    161 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    73 central nervous system, pupae P8    220 head, virgin 1-day female    138 head, virgin 4-day female    120 head, virgin 20-day female    59 head, mated 1-day female    141 head, mated 4-day female    93 head, mated 20-day female    52 head, mated 1-day male    222 head, mated 4-day male    194 head, mated 20-day male    161 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3  (73) central nervous system, pupae P8  (220) head, virgin 1-day female  (138) head, virgin 4-day female  (120) head, virgin 20-day female  (59) head, mated 1-day female  (141) head, mated 4-day female  (93) head, mated 20-day female  (52) head, mated 1-day male  (222) head, mated 4-day male  (194) head, mated 20-day male  (161) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    73 central nervous system, pupae P8  (220) head, virgin 1-day female  138 head, virgin 4-day female  120 head, virgin 20-day female    59 head, mated 1-day female  141 head, mated 4-day female    93 head, mated 20-day female    52 head, mated 1-day male  (222) head, mated 4-day male  (194) head, mated 20-day male  (161) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    73 central nervous system, pupae P8    220 head, virgin 1-day female    138 head, virgin 4-day female    120 head, virgin 20-day female    59 head, mated 1-day female    141 head, mated 4-day female    93 head, mated 20-day female    52 head, mated 1-day male    222 head, mated 4-day male    194 head, mated 20-day male    161 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    25 carcass, larvae L3 wandering    9 carcass, 1-day adult    11 carcass, 4-day adult    10 carcass, 20-day adult    8 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr    39 embryo 18-20hr    37 embryo 20-22hr    36 embryo 22-24hr    50 larva L1    35 larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP    27 pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr  (39) embryo 18-20hr  (37) embryo 20-22hr  (36) embryo 22-24hr  (50) larva L1  (35) larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP  (27) pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr    39 embryo 18-20hr    37 embryo 20-22hr    36 embryo 22-24hr    50 larva L1    35 larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP    27 pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr    39 embryo 18-20hr    37 embryo 20-22hr    36 embryo 22-24hr    50 larva L1    35 larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP    27 pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr    39 embryo 18-20hr    37 embryo 20-22hr    36 embryo 22-24hr    50 larva L1    35 larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP    27 pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr  (39) embryo 18-20hr  (37) embryo 20-22hr  (36) embryo 22-24hr  (50) larva L1  (35) larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP  27 pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr    39 embryo 18-20hr    37 embryo 20-22hr    36 embryo 22-24hr    50 larva L1    35 larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP    27 pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    5 embryo 12-14hr    12 embryo 14-16hr    20 embryo 16-18hr    39 embryo 18-20hr    37 embryo 20-22hr    36 embryo 22-24hr    50 larva L1    35 larva L2    12 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    23 pupae 3d postWPP    27 pupae 4d postWPP    19 adult male 01day    16 adult male 05day    15 adult male 30day    15 adult female 01day    7 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    0 embryonic S3    9 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    3 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult  (36) Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult  (36) Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    18 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_14 mE1_20_mRNA_expression_cluster_14
External Data & Images
Linkouts	BDGP expression data • LP05860 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005586 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7576-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rab3 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Rab3rup Sterility Allele fertile Rab3rup Other Phenotypes Allele neuroanatomy defective (with Df(2R)ED2076) Rab3rup neurophysiology defective (with Df(2R)ED2076) Rab3rup Phenotype manifest in Allele neuromuscular junction (with Df(2R)ED2076) Rab3rup t-bar (with Df(2R)ED2076) Rab3rup
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Rab3 Class Mutagen Stocks Known lesion Rab3c04828 piggyBac transposase 1 -- Rab3rup 0 Yes
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rab3 Class Mutagen Stocks Known lesion Rab3Q80L.Scer\UAS.P\T.T:Avic\GFP-YFP in vitro construct - amino acid replacement in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes Rab3Scer\UAS.P\T.T:Avic\GFP-YFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes Rab3HMS01131 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rab3JF02020 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rab3KK108633 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rab3Ncra\QUAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Rab3Ncra\QUAS.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Rab3T35N.Scer\UAS.P\T.T:Avic\GFP-YFP in vitro construct - amino acid replacement in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Rab3GD10603 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Rab3N134I.Scer\UAS in vitro construct - site directed mutagenesis 0 Yes Rab3NIG.7576R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Rab3Scer\UAS.cGa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)ED2076 (Graf et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10603} NA P{KK108633} NA P{NIG.7576R} NA P{TRiP.HMS01131} NA P{TRiP.JF02020} NA P{UASp-YFP.Rab3.Q80L} NA P{UASp-YFP.Rab3.T35N} NA P{UASp-YFP.Rab3} NA P{UAS-Rab3.G} NA P{UAS-Rab3.N134I} NA GAL4 construct PBac{I-SceI(FRT.Rab3-GAL4.ATG(loxP.3xP3-RFP))} No characterization construct P{QUAS-Rab3.eGFP} NA P{QUAS-Rab3.mCherry} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Rab3c04828 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
maintenance of presynaptic active zone structure inferred from mutant phenotype (Graf et al., 2009)
Cellular Component
CV term Evidence References
presynaptic active zone inferred from direct assay (Graf et al., 2009) colocalizes_with presynaptic active zone inferred from direct assay (Petersen and Stowers, 2011) synapse inferred from direct assay (Chan et al., 2011) synaptic vesicle inferred from direct assay (Chan et al., 2011) colocalizes_with synaptic vesicle inferred from direct assay (Petersen and Stowers, 2011)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
GTPase activity inferred from sequence or structural similarity (Kohno et al., 1994) inferred from sequence or structural similarity with mouse Rab3a (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1992.5.1) GTP binding inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003579, InterPro:IPR005225 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
neurotransmitter secretion non-traceable author statement (Lloyd et al., 2000) protein transport inferred from electronic annotation with InterPro:IPR003579 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1992.5.1) small GTPase mediated signal transduction inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003579 (FlyBase Curators et al., 2004-) vesicle-mediated transport non-traceable author statement (Lloyd et al., 2000)
Cellular Component
CV term Evidence References
synaptic vesicle non-traceable author statement (Lloyd et al., 2000, Swiss-Prot Project Members, 1992.5.1) vesicle inferred from sequence or structural similarity with Rab11 (Curators, 2008--)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Rab3 - CG7787 experimental knowledge based (Guruharsha et al., 2011) Rab3 - Cbs anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rab3 - Gdi experimental knowledge based (Guruharsha et al., 2011) Rab3 - Rab35 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rab3 allele Gene References unspecified ned (Burgess et al., 1997)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61936 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005586 DroID - A comprehensive database of gene and protein interactions. • FBgn0005586 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36127
Orthologs
OrthoDB Orthologs (113) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG63FHH6 Dipteran inclusive ortholog search EOG6DZ24B Insect inclusive ortholog search EOG68W9Z6 Arthropod inclusive ortholog search EOG6J6Q71 Metazoa inclusive ortholog search EOG66XB3W
Orthologs in Drosophila Species (EOG63FHH6)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Rab3     Drosophila simulans   Dsim\GD25963 Y   Drosophila sechellia   Dsec\GM20500 Y   Drosophila erecta   Dere\GG22724 Y   Drosophila yakuba   Dyak\GE13081 Y   Drosophila ananassae   Dana\GF11093 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20450 Y   Drosophila persimilis   Dper\GL17187 Y   Drosophila willistoni   Dwil\GK19661 Y   Drosophila virilis   Dvir\GJ20130 Y   Drosophila mojavensis   Dmoj\GI20173 Y   Drosophila grimshawi   Dgri\GH24197 Y
Orthologs in non-Drosophila Dipterans (EOG6DZ24B)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006267   Anopheles gambiae Malaria mosquito  Agam\AGAP005255   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010256
Orthologs in non-Dipteran Insects (EOG68W9Z6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB55960 Y Apis mellifera Western honey bee  Amel\Ame.1740 Y Apis mellifera Western honey bee  Amel\GB52842 Y Apis mellifera Western honey bee  Amel\Rab10 Y Apis mellifera Western honey bee  Amel\Ame.3590 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011245 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005975 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG020155 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001698 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013952 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18187 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH21022 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10637 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14030 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11347 Y Atta cephalotes Leafcutter ant  Acep\ACEP16947 Y Atta cephalotes Leafcutter ant  Acep\ACEP19570 Y Atta cephalotes Leafcutter ant  Acep\ACEP13274 Y Atta cephalotes Leafcutter ant  Acep\ACEP10256 Y Atta cephalotes Leafcutter ant  Acep\ACEP16079 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO16737 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17648 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17855 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO21657 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO20753 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19470 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18252 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17491 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20213 Y Linepithema humile Argentine ant  Lhum\LH12803 Y Linepithema humile Argentine ant  Lhum\LH20433 Y Linepithema humile Argentine ant  Lhum\LH20039 Y Linepithema humile Argentine ant  Lhum\LH19264 Y Linepithema humile Argentine ant  Lhum\LH15342 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB16634 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB12411 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB18086 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17524 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB22967 Y Solenopsis invicta Red fire ant  Sinv\SINV10886 Y Solenopsis invicta Red fire ant  Sinv\SINV24119 Y Solenopsis invicta Red fire ant  Sinv\SINV13339 Y Solenopsis invicta Red fire ant  Sinv\SINV19525 Y Solenopsis invicta Red fire ant  Sinv\SINV17605 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI000150 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001895 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001670 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002039 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI086839 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008058 Y Bombyx mori Silkmoth  Bmor\rabB Y Bombyx mori Silkmoth  Bmor\BGIBMGA004846 Y Bombyx mori Silkmoth  Bmor\BGIBMGA004666 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000295 Y Bombyx mori Silkmoth  Bmor\BGIBMGA002449 Y Pediculus humanus Human body louse  Phum\PHUM042170 Y Pediculus humanus Human body louse  Phum\PHUM046450 Y Pediculus humanus Human body louse  Phum\PHUM450640 Y Tribolium castaneum Red flour beetle  Tcas\TC001149 Y Tribolium castaneum Red flour beetle  Tcas\TC002919 Y Tribolium castaneum Red flour beetle  Tcas\TC014518 Y Tribolium castaneum Red flour beetle  Tcas\TC010990 Y Tribolium castaneum Red flour beetle  Tcas\TC009965 Y
Orthologs in non-Insect Arthropods (EOG6J6Q71)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\CYP2C31 Y Daphnia pulex Water flea  Dpux\JGI_V11_223052 Y Daphnia pulex Water flea  Dpux\JGI_V11_304534 Y Daphnia pulex Water flea  Dpux\JGI_V11_301441 Y Daphnia pulex Water flea  Dpux\JGI_V11_106641 Y Ixodes scapularis Deer tick  Isca\ISCW012427 Y Ixodes scapularis Deer tick  Isca\ISCW003929 Y Ixodes scapularis Deer tick  Isca\ISCW018125 Y Ixodes scapularis Deer tick  Isca\ISCW024874 Y Ixodes scapularis Deer tick  Isca\ISCW012955 Y
Orthologs in non-Arthropod Metazoa (EOG66XB3W)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rab-3   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000811gn   Danio rerio Zebrafish  Drer\rab3c   Danio rerio Zebrafish  Drer\rab3b   Danio rerio Zebrafish  Drer\rab3db   Danio rerio Zebrafish  Drer\rab3ab   Danio rerio Zebrafish  Drer\rab3aa   Danio rerio Zebrafish  Drer\rab3da   Xenopus tropicalis Western clawed frog  Xtro\rab3b   Xenopus tropicalis Western clawed frog  Xtro\rab3a   Xenopus tropicalis Western clawed frog  Xtro\rab3c   Xenopus tropicalis Western clawed frog  Xtro\rab3d   Gallus gallus Domestic chicken  Ggal\RAB3B   Gallus gallus Domestic chicken  Ggal\RAB3C   Mus musculus House mouse  Mmus\Rab3b   Mus musculus House mouse  Mmus\Rab3c   Mus musculus House mouse  Mmus\Rab3a   Rattus norvegicus Norway rat  Rnor\Rab3d   Rattus norvegicus Norway rat  Rnor\Rab3b   Rattus norvegicus Norway rat  Rnor\Rab3c   Rattus norvegicus Norway rat  Rnor\Rab3a   Homo sapiens Human  Hsap\RAB3D   Homo sapiens Human  Hsap\RAB3A   Homo sapiens Human  Hsap\RAB3B   Homo sapiens Human  Hsap\RAB3C
Human Orthologs (4)
Gene OMIM HGNC Hsap\RAB3D     Hsap\RAB3A     Hsap\RAB3B     Hsap\RAB3C
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25963 Drosophila sechellia Dsec\GM20500 Drosophila erecta Dere\GG22724 Drosophila yakuba Dyak\GE13081 Drosophila ananassae Dana\GF11093 Drosophila pseudoobscura pseudoobscura Dpse\GA20450 Drosophila persimilis Dper\GL17187 Drosophila willistoni Dwil\GK19661 Drosophila virilis Dvir\GJ20130 Drosophila mojavensis Dmoj\GI20173 Drosophila grimshawi Dgri\GH24197
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	31691 y1 v1; P{TRiP.JF02020}attP2 9764 y1 w*; P{UASp-YFP.Rab3.Q80L}11 9766 y1 w*; P{UASp-YFP.Rab3.T35N}04L 9762 y1 w*; P{UASp-YFP.Rab3}lolal02 34655 y1 sc* v1; P{TRiP.HMS01131}attP2 9765 y1 w*; P{UASp-YFP.Rab3.Q80L}wmd29a 9763 y1 w*; P{UASp-YFP.Rab3}4EHP05b/TM3, Sb1 36352 y1 w*; P{QUAS-Rab3.eGFP}attP40; Dr1/TM6C, Sb1 36353 y1 w*; P{QUAS-Rab3.mCherry}attP40/CyO, P{Wee-P.ph0}BaccWee-P20; Dr1/TM6C, Sb1
Harvard	- PBac{PB}Rab3c04828
VDRC	v100787 P{KK108633}VIE-260B
Genomic Clones ( 1 )
	BACR08L17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 259 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP05860
Other clones	DPiM_LP05860
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH06611 GH13464 GH27544 HL01307 LD11649 LP16880 RE06284 RE07792 RE08796 RE09344 RE21129 RE22005 RE35244 RE42409 RE43835 RE59682 RE64276 RH46388 RH66552
Other clones	11515 28097 28479 31008 32909 36445 38807 43431 53422 62303 64321 80770 81283 83344 99414 108336 108522 161544 163921 174691 174706 CK01.52A.C6 CK01.121B.G3 CK01.122A.A4 EC052726 EC052751 EC053220 EC053961 EC054722 EC056113 EK042243 EK093234 EK111726 EK136412 EK143310 EK144650 EK152651 EK156130 EK160207 EK163062 EK188126 EK191262 EK213826 EK214664 EK222042 EK234312 EK265836 EK267820 EK268336 EK275657 EK279163 EK284605 RP003004701 RP003008072 RP003008529 RP003018004 RP003020321 RP003021455 RP003022480 RP003027506 RP003029381 RP003067416 RP003068753 RP003069419 RP003082445 RP003084965 RP003086853 RP003100866 RP003101057 RP003101545 RP003114044 RP003121590 RP003124053 RP003129368 RP003130344 RP003132018 RP003136341 RP003137182 RP003141576 RP003141630 RP003147075 RP003152155 RP003152524 RP003155820 RP003162917 RP003163450 RP003176979 RP003183124 RP003184567 RP003188486 RP003191293 RP003192698 RP003196212 RP003203973 RP003216047 RP003220004 RP003220053 RP003225776 RP003226233 RP003226478 RP003229020 RP003229591 RP003234295 RP003238655 RP003239472 RP003241273 RP003241626 RP003242663 RP003242874 RP003250367 RP003252550 RP003253845 RP003259259 RP003263473 RP003266551 RP003267645 RP003268928 RP003276578 RP003277072 RP003279397 RP003281916 RP003290447 RP003292929 RP003295178 RP003296043 RP003299165 RP003300447 RP003301360 RP003307747 RP003308299 RP003311004 RP003314685 RP003314786 RP003322434 RP003323112 RP003328275 RP003328390 RP003330101 RP003330327 RP003336370 RP003340161 RP003344870 RP003346317 RP003347119 RP003351178 RP003352827 RP003356687 RP003373354 RP003379346 RP003383524 RP003388033 RP003388928 RP003389085 RP003392981 RP003401308 RP003406435 RP003416082 RP003417625 RP003420561 RP003422357 RP003423058 RP003425194 RP003438613 RP003441378 RP003448821 RP003452697 RP003453570 RP003455641 RP003460569 RP003463017 RP003472313 RP003473758 RP003474892 RP003475848 RP003477646 RP003477823 RP003481874 RP003482178 RP003489239 RP003489435 RP003491880 RP003492823 RP003493256 RP003494624 RP003497293 RP003497616 RP003504784 RP003516888 RP003518405 RP003520049 RP003523775 RP003532666 RP003532946 RP003538944 RP003544376 RP003549566 RP003556208 RP003560228 RP003564737 RP003570777 RP003575009 RP003581292 RP003586489 RP003589103 RP003593914 RP003594017 RP003595944 RP003596957 RP003601595 RP003604133 RP003605712 RP003613123 RP003614369 RP003618085 RP003626209 RP003626323 RP003629983 RP003630052 RP003630102 RP003633889 RP003636907 RP003637702 RP003637878 RP003639387 RP003640646 RP003641436 RP003641662 RP003643604 RP003659503 RP003661498 RP003664460 RP003665398 RP003667427 RP003667689 RP003668069 RP003670099 RP003672294 RP003679441
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0005586 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36127
Antibody Information
	polyclonal (Graf et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Rab3 is not required for the rapid induction or the sustained expression of synaptic homeostasis. (Müller et al., 2011) Rab3 apears necessary for normal morphological synapse development but opposes the expression of homeostatic plasticity. (Müller et al., 2011) dsRNA has been made from templates generated with primers directed against this gene. (Kouranti et al., 2006) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Evolutionary conservation of both structure and localisation suggests that n-syb, Rab3 and syt have an important function in the synaptic vesicle. (DiAntonio et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078963 RefSeq (Proteins) • NP_523687 DDBJ / EMBL / GenBank • AAA28843 AAF58762 AAL25488 AB112932 AI257478 AW944055 AY060449 BAD07037 CZ469537 M64621 Z50312 Entrez Gene - A searchable database of RefSeq genes. • 36127 UniProtKB/Swiss-Prot • P25228
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Small GTPase superfamily (IPR001806) Small GTPase superfamily, Rab type (IPR003579) Small GTP-binding protein domain (IPR005225) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LP05860
Linkouts
	BioGRID - A database of protein and genetic interactions • 61936 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005586 DroID - A comprehensive database of gene and protein interactions. • FBgn0005586 DRSC - Results from RNAi screens. • FBgn0005586 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005586 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7576-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0005586 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36127 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36127 modMine - Data generated by the modENCODE project. • FBgn0005586
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	AAF58762 (Armstrong, 2000) CG7576 (Zhang et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2008, Ni et al., 2010.12.1, Colicelli, 2004) dm-Rab3 (Fukuda et al., 2004, Fukuda, 2004) DmRab3 (Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001) DRab3 (Fabian-Fine et al., 2003) drab3 (Lloyd et al., 2000, Burgess and Schwarz, 1993, DiAntonio et al., 1993) Drab3 (Satoh et al., 1997, Parfitt et al., 1995, ) DRAB3 (Kohno et al., 1994) P25228 (Rojas et al., 2012) rab3 (Chae et al., 1999, Tchurikov et al., 1998, Burgess et al., 1995, Johnston et al., 1991, Fukuda, 2003.6.21, Chan et al., 2011, Graf et al., 2009, Jin et al., 2012) Rab3 (Kouranti et al., 2006, Fukuda, 2004, Cheng et al., 2011, Zhang et al., 2007, Zhang et al., 2007, Bakal et al., 2007, Thomas et al., 2009, Nechipurenko and Broihier, 2012, Monnier et al., 2002, Graf et al., 2012, Müller et al., 2011, Wu et al., 2005, Peled and Isacoff, 2011, Petersen and Stowers, 2011, Japanese National Institute of Genetics, 2012.5.21, Nfonsam et al., 2012) Rab3A (Littleton et al., 1998)
Name Synonym	Rab3   Rab-protein 3
Secondary FlyBase IDs
References ( 68 )
Generate a list of	All references relating to this gene ( 68 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 10 ) Review  ( 4 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 5 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Letter  ( 1 )
Recent research papers ( 10 )
	Graf et al., 2012, J. Neurosci. 32(47): 16586--16596 RIM Promotes Calcium Channel Accumulation at Active Zones of the Drosophila Neuromuscular Junction. [FBrf0220028] Jin et al., 2012, PLoS ONE 7(7): e40912 Similarities of Drosophila rab GTPases Based on Expression Profiling: Completion and Analysis of the rab-Gal4 Kit. [FBrf0219021] Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] Nfonsam et al., 2012, PLoS ONE 7(8): e44583 Analysis of the Transcriptomes Downstream of Eyeless and the Hedgehog, Decapentaplegic and Notch Signaling Pathways in Drosophila melanogaster. [FBrf0219414] Chan et al., 2011, Curr. Biol. 21(20): 1704--1715 Systematic discovery of rab GTPases with synaptic functions in Drosophila. [FBrf0216517] Cheng et al., 2011, J. Cell Biol. 194(6): 921--935 S6 kinase localizes to the presynaptic active zone and functions with PDK1 to control synapse development. [FBrf0216911] Müller et al., 2011, Neuron 69(4): 749--762 Rab3-GAP Controls the Progression of Synaptic Homeostasis at a Late Stage of Vesicle Release. [FBrf0213055] Peled and Isacoff, 2011, Nat. Neurosci. 14(4): 519--526 Optical quantal analysis of synaptic transmission in wild-type and rab3-mutant Drosophila motor axons. [FBrf0213336] Petersen and Stowers, 2011, PLoS ONE 6(9): e24531 A Gateway MultiSite Recombination Cloning Toolkit. [FBrf0215584] Uytterhoeven et al., 2011, Cell 145(1): 117--132 Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins. [FBrf0213384] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...