The gene trp-like is referred to in FlyBase by the symbol Dmel\trpl (CG18345, FBgn0005614). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; calmodulin binding; protein heterodimerization activity; calcium channel activity; light-activated voltage-gated calcium channel activity. There is experimental evidence that it is involved in the biological process: sensory perception of sound; calcium-mediated signaling; calcium ion transmembrane transport; phototransduction, visible light; body fluid secretion; calcium ion transport; cellular response to anoxia. 22 alleles are reported. The phenotypes of these alleles are annotated with: photoreceptor; photoreceptor cell; Malpighian tubule; eye photoreceptor cell. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Ankyrin repeat; Ankyrin repeat-containing domain; Ion transport domain; Transient receptor ion channel domain; Transient receptor potential channel; Transient receptor potential channel, canonical. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye. Comments on Affy2 ProbeSet: ProbeSet 1633649_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of trpl. Gene sequence location is 2R:5641202..5653023.
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Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye.
[download data (TSV)]
Guide to FlyAtlas expression level colors
No expression (0 - 9.999)
Low expression (10 - 99.999)
Moderate expression (100 - 499.999)
High level expression (500 - 999.999)
Very high expression (>999.999)
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
2511.2
Adult Eye
6845.775
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
(2511.2)
Adult Eye
(6845.775)
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
(2511.2)
Adult Eye
(6845.775)
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
2511.2
Adult Eye
6845.775
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
2511.2
Adult Eye
6845.775
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
(2511.2)
Adult Eye
(6845.775)
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
(2511.2)
Adult Eye
(6845.775)
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
8.275
Larval Midgut
7.7
Larval Hindgut
6.1
Larval Malpighian Tubules
8.4
Larval Fat Body
16.2
Larval Salivary Gland
6.3
Larval Trachea
219.825
Larval Carcass
16.075
Adult Head
2511.2
Adult Eye
6845.775
Adult Brain
73.7
Adult Thoracic-Abdominal Ganglion
11.6
Adult Crop
11.4
Adult Midgut
19.1
Adult Hindgut
12
Adult Malpighian Tubules
6.7
Adult Fat Body
12.4
Adult Salivary Gland
21.5
Adult Heart
13.55
Adult VirginFemale Spermatheca
9.5
Adult InseminatedFemale Spermatheca
11.9
Adult Ovary
8.4
Adult Testis
13.5
Adult Male Accessory Gland
35.3
Adult Carcass
16.2
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed in adult male stages.
[download data (TSV)]
Please Note FlyBase no
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may not be complete
cDNA Clones ( 133 )
Please Note
This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see GBrowse for alignment of the cDNAs and ESTs
to the gene model.
Both the N-terminal and C-terminal regions of trpl are necessary for the specific light-dependent translocation that is typical of the trpl ion channel.
trpl protein is translocated in a light-dependent manner between the rhabdomere photoreceptor membrane and an intracellular storage channel. The level of trpl protein in the rhabdomere is high in the dark and low in the light.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.
The trp and trpl channels are specific targets of metabolic stress; anoxia opens trp and trpl channels in the dark. Mitochondrial uncouplers and depletion of ATP activate the trp and trpl channels in the dark in situ.
The fact that the ability of non-inaD bound trp to contribute to photoreceptor responses depends on the presence of trpl suggests that there may be interactions between trpl and the non-inaD-bound trp protein.
The biophysical properties of trpl channels expressed in a stably transfected cell line are compared to those of trp. Results show that, for a wide range of properties, the native and expressed channels are functionally equivalent lending strong support to the proposal that channels encoded by trpl can completely account for the component of the light-sensitive conductance remaining in the trp mutant.
trpl expression is associated with the appearance of an outwardly rectifying, Ca2+ permeable, nonselective cation current that is constitutively active under nonstimulating conditions, giving rise to large increases in basal Ca2+.
Wild-type light activated conductance is composed of two separate channels, with the properties of trp- and trpl-encoded channels as determined in the respective mutants.
Coexpression of trp and trpl leads to a store-operated, outwardly rectifying current distinct from that owing to either trp or trpl alone. trp and trpl proteins interact directly, indicating the trp-trpl-dependent current is mediated by heteromultimeric association between the two subunits.
trpl activity is masked by functional trp channels and together trp and trpl are essential components of the light-activated conductance. trp and trpl need not form heteromeric channels because trpl mutants only contain trp protein and trp mutants only have trpl protein. Since trp and trpl mutants respond to light activation then each channel on its own must be capable of sensing the intracellular messenger that gates the light activated conductance. Activation of one does not require prior activation of the other.
Analysis of trp-trpl fusion proteins suggests that the carboxy terminal domain of trpl plays an important role in determining constitutive activity of the protein, and the carboxy terminal domain of trp contains structural features necessary for activation by thapsigargin.
trpl expressed in Sf9 cells can be activated by hormonal factors after coinjection with recombinant viruses coding for various heptahelical, G-protein-coupled receptors.
Mutants in Drosophila TRPC Channels Reduce Olfactory Sensitivity to Carbon Dioxide. [FBrf0220002]
Hardie and Franze, 2012, Science 338(6104): 260--263
Photomechanical responses in Drosophila photoreceptors. [FBrf0219673]
Lev et al., 2012, J. Biol. Chem. 287(2): 1436--1447
Signal-dependent Hydrolysis of Phosphatidylinositol 4,5-Bisphosphate without Activation of Phospholipase C: IMPLICATIONS ON GATING OF DROSOPHILA TRPL (TRANSIENT RECEPTOR POTENTIAL-LIKE) CHANNEL. [FBrf0217165]
Lev et al., 2012, Channels 6(2): 86--93
The activity of the TRP-like channel depends on its expression system. [FBrf0218631]
Lieu et al., 2012, PLoS ONE 7(2): e31622
Mechanisms Underlying Stage-1 TRPL Channel Translocation in Drosophila Photoreceptors. [FBrf0217631]
Senthilan et al., 2012, Cell 150(5): 1042--1054
Drosophila auditory organ genes and genetic hearing defects. [FBrf0219321]
Oberegelsbacher et al., 2011, Europ. J. Cell Biol. 90(8): 620--630
The Drosophila TRPL ion channel shares a Rab-dependent translocation pathway with rhodopsin. [FBrf0213847]
Petersen and Stowers, 2011, PLoS ONE 6(9): e24531
A Gateway MultiSite Recombination Cloning Toolkit. [FBrf0215584]
Richter et al., 2011, J. Biol. Chem. 286(39): 34234--34243
Translocation of the Drosophila Transient Receptor Potential-like (TRPL) Channel Requires Both the N- and C-terminal Regions Together with Sustained Ca2+ Entry. [FBrf0216251]
Rosenbaum et al., 2011, Neuron 72(4): 602--615
XPORT-Dependent Transport of TRP and Rhodopsin. [FBrf0216705]
Seong et al., 2011, Biogerontology 12(2): 93--107
Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity. [FBrf0213307]
Vasiliauskas et al., 2011, Nature 479(7371): 108--112
Feedback from rhodopsin controls rhodopsin exclusion in Drosophila photoreceptors. [FBrf0216554]
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