• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\fln

General Information
Symbol	Dmel\fln	Species	D. melanogaster
Name	flightin	Annotation symbol	CG7445
Feature type	protein_coding_gene	FlyBase ID	FBgn0005633
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	mp27
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	76D8-76D8	Sequence location	3L:19,982,651..19,984,279 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene flightin is referred to in FlyBase by the symbol Dmel\fln (CG7445, FBgn0005633). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: adult somatic muscle development; skeletal muscle myosin thick filament assembly; striated muscle myosin thick filament assembly. 5 alleles are reported. The phenotypes of these alleles are annotated with: dorsal medial muscle; wing; indirect flight muscle. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of no expression detected. Peak expression observed during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637335_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of fln. Gene sequence location is 3L:19982651..19984279.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features BKN30457 HFA11346 References Schönbauer et al., 2011, Nature 479(7373): 406--409
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 76D8-76D8   Limits computationally determined from genome sequence between P{lacW}l(3)L1243L1243&P{lacW}Mi-2j3D4 and P{PZ}eRF100103
Experimentally Determined Cytological Location
Cytogenetic map Notes References 76D-76D   (determined by in situ hybridisation)   (Vigoreaux et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes	Maps 23.1 map units from e. (Reedy et al., 2000)
Gene Model & Products
Please see the GBrowse view of Dmel\fln for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) fln-RA FBtr0074910  NM_079450   987   182
Additional Transcript Data & Comments
Reported size (kB)	1.0 (northern blot) (Vigoreaux et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein fln-PA   FBpp0074679   20.7   182   5.12     NP_524174   AAF49084
Additional Polypeptide Data & Comments
Reported size (kDa)	182 (aa); 20 (kD) (Vigoreaux et al., 1993)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF49084   AI513729   AW941029   AY060802 AAL28350   CO272966   CO284752   CO314738   CO340301   EC075241   EC209301   EC237444   EC255826   EC256185   EV581118   EV585150   EV587224   Z18858 CAA79309
UniProtKB/Swiss-Prot	P35554
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference pupal stage     (Vigoreaux et al., 1993) in situ Stage Tissue/Position (including subcellular localization) Reference adult stage   indirect flight muscle   (Vigoreaux et al., 1993)
Additional Descriptive Data
	fln transcripts are detected in late pupae and adults. They localize exclusively to indirect flight muscles in adult flies. (Vigoreaux et al., 1993)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference adult stage   indirect flight muscle   (Vigoreaux et al., 1993) enzyme assay or biochemical detection Stage Tissue/Position (including subcellular localization) Reference pupal stage     (Vigoreaux et al., 1993) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	fln protein localizes specifically to the A band of myofibrils. (Vigoreaux et al., 1993)
Marker for
Subcellular Localization
CV Term	A band (Vigoreaux et al., 1993)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0005633    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0005633 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of no expression detected. Peak expression observed during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0005633 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP    9461 pupae 3d postWPP    145503 pupae 4d postWPP    167083 adult male 01day    72642 adult male 05day    10142 adult male 30day    1460 adult female 01day    62227 adult female 05day    2528 adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr  (110) embryo 22-24hr  (110) larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new  (708) white prepupae 12hr  (925) white prepupae 24hr    12 pupae 2d postWPP  (9461) pupae 3d postWPP  (145503) pupae 4d postWPP  (167083) adult male 01day  (72642) adult male 05day  (10142) adult male 30day  (1460) adult female 01day  (62227) adult female 05day  (2528) adult female 30day  (560) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP  (9461) pupae 3d postWPP  (145503) pupae 4d postWPP  (167083) adult male 01day  (72642) adult male 05day  (10142) adult male 30day  1460 adult female 01day  (62227) adult female 05day  (2528) adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP    9461 pupae 3d postWPP  (145503) pupae 4d postWPP  (167083) adult male 01day  (72642) adult male 05day  10142 adult male 30day    1460 adult female 01day  (62227) adult female 05day    2528 adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP    9461 pupae 3d postWPP    145503 pupae 4d postWPP    167083 adult male 01day    72642 adult male 05day    10142 adult male 30day    1460 adult female 01day    62227 adult female 05day    2528 adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0005633 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP    9461 pupae 3d postWPP    145503 pupae 4d postWPP    167083 adult male 01day    72642 adult male 05day    10142 adult male 30day    1460 adult female 01day    62227 adult female 05day    2528 adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr  110 embryo 22-24hr  110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new  (708) white prepupae 12hr  (925) white prepupae 24hr    12 pupae 2d postWPP  (9461) pupae 3d postWPP  (145503) pupae 4d postWPP  (167083) adult male 01day  (72642) adult male 05day  (10142) adult male 30day  (1460) adult female 01day  (62227) adult female 05day  (2528) adult female 30day  (560) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP  (9461) pupae 3d postWPP  (145503) pupae 4d postWPP  (167083) adult male 01day  (72642) adult male 05day  (10142) adult male 30day  1460 adult female 01day  (62227) adult female 05day  (2528) adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP    9461 pupae 3d postWPP  (145503) pupae 4d postWPP  (167083) adult male 01day  (72642) adult male 05day  10142 adult male 30day    1460 adult female 01day  (62227) adult female 05day    2528 adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    3 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    8 embryo 12-14hr    10 embryo 14-16hr    28 embryo 16-18hr    55 embryo 18-20hr    63 embryo 20-22hr    110 embryo 22-24hr    110 larva L1    75 larva L2    29 larva L3 12hr old    21 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    20 white prepupae new    708 white prepupae 12hr    925 white prepupae 24hr    12 pupae 2d postWPP    9461 pupae 3d postWPP    145503 pupae 4d postWPP    167083 adult male 01day    72642 adult male 05day    10142 adult male 30day    1460 adult female 01day    62227 adult female 05day    2528 adult female 30day    560 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0005633    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0005633 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0005633 expression level Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass    2113.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass  (2113.5) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass  (2113.5) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass    2113.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0005633 expression level Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass    2113.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass  (2113.5) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass  (2113.5) Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    7.625 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.5 Larval Fat Body    3.5 Larval Salivary Gland    2.4 Larval Trachea    2.975 Larval Carcass    7.725 Adult Head no informative data Adult Eye    2.875 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    27.4 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut    6.1 Adult Malpighian Tubules    5.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2.2 Adult Testis    3 Adult Male Accessory Gland    9.8 Adult Carcass    2113.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    2.875   NA  Brain    no informative data   7.625  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    27.4   NA  Crop    no informative data   no informative data  Midgut    5.6   no informative data  Hindgut    6.1   7.5  Malpighian Tubules    5.6   3.5  Fat Body    no informative data   2.4  Salivary Gland    no informative data   NA  Heart    no informative data   2.975  Trachea    NA   NA  VirginFemale Spermatheca    8.5   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    2.2   NA  Testis    3   NA  Male Accessory Gland    9.8   7.725  Carcass    2113.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_00
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005633 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7445-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable fln0 viable, with Scer\GAL4pnr-MD237 flnGD10601 Sterility Allele fertile fln0 Other Phenotypes Allele flight defective, with Scer\GAL4Mef2.PR flnGD10601 flightless fln0 flightless (with Df(3L)fln1) fln0 flightless | recessive fln0 locomotor behavior defective, with Scer\GAL4Mef2.PR flnGD10601 visible fln0 Phenotype manifest in Allele dorsal medial muscle fln0 dorsal medial muscle & myofibril fln0 dorsal medial muscle & sarcomere fln0 dorsal medial muscle & striated muscle thick filament fln0 dorsal medial muscle & striated muscle thin filament fln0 indirect flight muscle fln0 indirect flight muscle, with Scer\GAL4Mef2.PR flnGD10601 indirect flight muscle & muscle tendon junction fln0 indirect flight muscle & myofibril fln0 indirect flight muscle & sarcomere fln0 indirect flight muscle & striated muscle thick filament fln0 indirect flight muscle & striated muscle thin filament fln0 indirect flight muscle & Z disc fln0 wing fln0
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of fln Class Mutagen Stocks Known lesion fln0 ethyl methanesulfonate 1 Yes flnMB03038 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of fln Class Mutagen Stocks Known lesion flnGD10601 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes flnKK110457 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes flnAct88F.PB in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)fln1 (Vigoreaux et al., 1998)
Not disrupted in	Df(3L)in61 (Hernandez et al., 1996) Df(3L)kto2 (Hernandez et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10601} NA P{KK110457} NA characterization construct P{Act88F-fln.B} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}CG7646BG02179 NA insertion of enhancer trap binary system Mi{ET1}flnMB03038 No
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
adult somatic muscle development inferred from mutant phenotype (Schnorrer et al., 2010) skeletal muscle myosin thick filament assembly inferred from mutant phenotype (Reedy et al., 2000) striated muscle myosin thick filament assembly inferred from direct assay (Contompasis et al., 2010)
Cellular Component
CV term Evidence References
A band inferred from direct assay (Vigoreaux et al., 1993) striated muscle myosin thick filament inferred from direct assay (Vigoreaux, 1994, Reedy et al., 2000)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
contractile fiber non-traceable author statement (Swiss-Prot Project Members, 1994.6.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions fln allele Gene References fln0 Act88F (Reedy et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65452 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005633 DroID - A comprehensive database of gene and protein interactions. • FBgn0005633 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40185
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10833 Dere\GG13353 Dgri\GH14726 Dmoj\GI13378 Dper\GL25050 Dpse\GA22938 Dsec\GM14859 Dsim\GD12234 Dvir\GJ11502 Dwil\GK18981 Dyak\fln
Curated drosophilid orthologs	Dyak\fln
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004249-PA Aedes aegypti (mosquito) AGAP007249-PA Anopheles gambiae (mosquito) XP_001121224.1 Apis mellifera (honey bee) XP_001944120.1 Acyrthosiphon pisum (pea aphid) BGIBMGA000738-PA Bombyx mori (silk worm) CPIJ008221 Culex pipens (mosquito) 227136 Daphnia pulex (daphnia) XP_001607812.1 Nasonia vitripennis (wasp) PHUM010784-PA Pediculus humanus (human body louse) XP_969293.1 Tribolium castaneum (red flour beetle) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13031 NV10917 LH11529 FBgn0067972 FBgn0220979 FBgn0198760 FBgn0183970 FBgn0169780 JGI_V11_227136 FBgn0244340 FBgn0162637 FBgn0136135 FBgn0122202 FBgn0105625 FBgn0087873 CPIJ008221 BGIBMGA000738 ACYPI002594 AGAP007249 AAEL004249 PHUM538600
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	6655 w1; fln0 e1 23601 w1118; Mi{ET1}flnMB03038/TM3, Sb1 Ser1
VDRC	v101988 P{KK110457}VIE-260B v46153 w1118; P{GD10601}v46153
Genomic Clones ( 2 )
	BACR10L01 BACR13N10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 14 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH26786
Other clones	DPiM_GH26786
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	9970 73866 118070 214281.39 214481.53 Dmel_dig_012253_0654_3287 Dmel_dig_017668_0614_3577 Dmel_neb_013299_0192_0535 EK109010 EK166710 EK237645 EP10361
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0005633 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40185
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	fln has an essential role in defining the structural and mechanical properties of indirect flight muscle thick filaments. The absence of fln results in unstable thick filaments of abnormal length. (Contompasis et al., 2010) fln is required for the establishment of normal thick filament length in the indirect flight muscle during late pupal development and for thick filament stability in the adult after initiation of contractile activity. (Reedy et al., 2000) fln is a structural component of the thick filaments whose regulated phosphorylation is dependent upon the presence of fully assembled thin filaments or the activity of thin filament protein. (Vigoreaux, 1994) Flightin exists in 11 isoelectric variants, of both phosphorlated and non-phosphorylated classes. There is a dramatic increase in flightin phosphorylation on adult eclosion. (Vigoreaux and Perry, 1994) Flightin is a protein of the A band of the sarcomere of the indirect flight muscles. Exists in various, probably post-translationally modified, isoforms (kD 27-30; pI 4.6-6.0). Isoform pattern changes after emergence. Probably interacts with myosin, as the 12 N-terminal amino acids are similar to those of actin, which also interacts with myosin. (Vigoreaux et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079450 RefSeq (Proteins) • NP_524174 DDBJ / EMBL / GenBank • AAF49084 AAL28350 AI513729 AW941029 AY060802 CAA79309 Z18858 Entrez Gene - A searchable database of RefSeq genes. • 40185 UniProtKB/Swiss-Prot • P35554
Other Crossreferences
Linkouts
	BioGRID - A database of protein and genetic interactions • 65452 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005633 DroID - A comprehensive database of gene and protein interactions. • FBgn0005633 DRSC - Results from RNAi screens. • FBgn0005633 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005633 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7445-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0005633 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40185 InParanoid A subset of ortholog calls from InParanoid. • AAEL004249-PA Aedes aegypti (mosquito) AGAP007249-PA Anopheles gambiae (mosquito) XP_001121224.1 Apis mellifera (honey bee) XP_001944120.1 Acyrthosiphon pisum (pea aphid) BGIBMGA000738-PA Bombyx mori (silk worm) CPIJ008221 Culex pipens (mosquito) 227136 Daphnia pulex (daphnia) XP_001607812.1 Nasonia vitripennis (wasp) PHUM010784-PA Pediculus humanus (human body louse) XP_969293.1 Tribolium castaneum (red flour beetle) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40185 modMine - Data generated by the modENCODE project. • FBgn0005633 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13031 NV10917 LH11529 FBgn0067972 FBgn0220979 FBgn0198760 FBgn0183970 FBgn0169780 JGI_V11_227136 FBgn0244340 FBgn0162637 FBgn0136135 FBgn0122202 FBgn0105625 FBgn0087873 CPIJ008221 BGIBMGA000738 ACYPI002594 AGAP007249 AAEL004249 PHUM538600
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG7445 (Lebo et al., 2009, Keleman et al., 2009.8.5, Cammarato et al., 2011) flightin (Contompasis et al., 2010) fln (Reiter et al., 2007, Montana and Littleton, 2006, Maines et al., 2007, Domazet-Loso and Tautz, 2003, Dietzl et al., 2007, Henkin et al., 2004, Barton et al., 2005, Carpenter et al., 2009, Schnorrer et al., 2010, Contompasis et al., 2010, Cherbas et al., 2011) Fln (Qiu et al., 2005, Clayton et al., 1998, McGraw et al., 2008) Ftn   mp27 (Sparrow et al., 1995, Vigoreaux et al., 1991, )
Name Synonym	flightin (Pardue, 1992.12.1, Qiu et al., 2005, Clayton et al., 1998, Henkin et al., 2004, McGraw et al., 2008, Lebo et al., 2009, Barton et al., 2005, Schnorrer et al., 2010, Contompasis et al., 2010, Manh et al., 2005, Cammarato et al., 2011) Flightin
Secondary FlyBase IDs
	FBgn0010036
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 3 ) Review  ( 3 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 12 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 4 )
	Cherbas et al., 2011, Genome Res. 21(2): 301--314 The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077] Schönbauer et al., 2011, Nature 479(7373): 406--409 Spalt mediates an evolutionarily conserved switch to fibrillar muscle fate in insects. [FBrf0216735] Contompasis et al., 2010, J. Mol. Biol. 395(2): 340--348 Flightin is necessary for length determination, structural integrity, and large bending stiffness of insect flight muscle thick filaments. [FBrf0209850] Schnorrer et al., 2010, Nature 464(7286): 287--291 Systematic genetic analysis of muscle morphogenesis and function in Drosophila. [FBrf0210226] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...