FlyBase Gene Report: Dmel\slbo

posterior spiracle primordium

(Montell et al., 1992)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

Comment: reported as posterior spiracle specific anlage

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval posterior spiracle

(Fisher et al., 2012)

adult stage & oogenesis

(Montell et al., 1992)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis | female

ovary

(Rorth and Montell, 1992)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

adult stage & oogenesis stage S10

(Borghese et al., 2006)

Additional Descriptive Data

slbo is enriched in border follicle cells relative to follicle cells.

(Borghese et al., 2006)

Expression of slbo is seen in border follicle cells prior to, during and after their migration and in centripetally migrating follicle cells at stage S10.

(Montell et al., 1992)

Northern blot analysis shows that slbo transcripts are most abundant in embryos but are also found in second instar larvae and in ovaries. Primer extension analysis shows that the transcripts are first detected in 8 hr embryos and peak at 12-16 hr.

(Rorth and Montell, 1992)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic/larval anterior spiracle

(Montell et al., 1992)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13

Comment: 9-10 hr AEL

(Rorth and Montell, 1992)

embryonic stage 15

fused midgut primordium

(Rorth and Montell, 1992)

embryonic proventriculus

(Rorth and Montell, 1992)

embryonic foregut

(Rorth and Montell, 1992)

embryonic hindgut

(Rorth and Montell, 1992)

presumptive embryonic salivary gland

(Rorth and Montell, 1992)

epidermis

(Rorth and Montell, 1992)

oogenesis stage S8 & adult stage | female

(Szafranski and Goode, 2004)

adult stage oogenesis stage S9 -- S10

(Montell et al., 1992)

oogenesis stage S8 -- S10

(Cobreros et al., 2008)

oogenesis stage S10A

embryonic/larval posterior spiracle

(Levine et al., 2010)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 17

embryonic hindgut

(Rorth and Montell, 1992)

• nucleus

(Rorth and Montell, 1992)

embryonic stage 15 -- 16

embryonic midgut constriction

• nucleus

(Rorth and Montell, 1992)

embryonic stage 15 -- 17

presumptive embryonic salivary gland

• nucleus

(Rorth and Montell, 1992)

embryonic proventriculus

• nucleus

(Rorth and Montell, 1992)

embryonic foregut

(Rorth and Montell, 1992)

embryonic stage 17

embryonic epidermis | anterior | posterior

(Rorth and Montell, 1992)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 16

organism

Comment: reference states 12-16 hr AEL

(Rorth and Montell, 1992)

Additional Descriptive Data

Transient slbo expression is observed in the centripetally migrating follicle cells at oogenesis stage S10A and is undetectable by stage 10B. Expression in leading edge follicle cells is not observed with the antisera.

(Levine et al., 2010)

Expression of slbo is first detected in border follicle cells in early stage S9 just prior to their migration and in centripetally migrating follicle cells at stage S10 prior to their migration. No staining is visible after stage S10. Staining of border and centripetal follicle cells was greatly reduced or absent in slbo mutants.

(Montell et al., 1992)

Western blots show a peak of slbo protein expression in 12-18hr embryos. Immunolocalization studies show the earliest detectable expression is in the posterior spiracles of 9-10hr embryos. After 12hrs, a rapidly changing pattern is seen. Expression is seen first in the proventriculus, salivary glands and midgut followed by the foregut, hindgut and the epidermis. Epidermal staining is initially fairly ubiquitous but becomes restricted to the ends of the embryo and a few epidermal cells in the intermediate segments.

(Rorth and Montell, 1992)

Marker for

(Gunawan et al., 2013, Masoner et al., 2013, Cobreros et al., 2008, Szafranski and Goode, 2004)

(Masoner et al., 2013)

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Rorth and Montell, 1992)

Expression Deduced from Reporters

Reporter: P{GAL4-slbo.2.6}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

(Rorth et al., 1998)

(Rorth et al., 1998)

adult stage | female oogenesis stage S9 -- S10

(Starz-Gaiano et al., 2008)

adult stage & oogenesis stage S10

(Starz-Gaiano et al., 2008)

Reporter: P{PZ}slbo⁰¹³¹⁰

Stage

Tissue/Position (including subcellular localization)

Reference

(Rorth et al., 1998)

follicle cell

(Rorth et al., 1998)

oogenesis stage S8 -- S10 & adult stage | female

(Gunawan et al., 2013)

oogenesis stage S9 -- S10 & adult stage | female

(Wang et al., 2007)

oogenesis stage S9 & adult stage | female

(Lucas et al., 2013)

oogenesis stage S9 -- S14

(Levine et al., 2010)

oogenesis stage S10A

(Levine et al., 2010)

stretch follicle cell

(Levine et al., 2010)

oogenesis stage S11

wandering third instar larval stage

Comment: leading edge

(Levine et al., 2010)

Reporter: P{ST.lexA::HG}SX-1

Stage

Tissue/Position (including subcellular localization)

Reference

eye disc

(Bloomington Drosophila Stock Center, 2019)

larval central nervous system

(Bloomington Drosophila Stock Center, 2019)

larval ventral nerve cord

(Bloomington Drosophila Stock Center, 2019)

larval enteroendocrine cell

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval garland cell

(Bloomington Drosophila Stock Center, 2019)

larval pericardial cell

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval salivary gland

(Bloomington Drosophila Stock Center, 2019)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\slbo in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\slbo

View exonic expression by tissue for Dmel\slbo

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0005638

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37889/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0005638

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0005638

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0005638

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4354

Flygut - An atlas of the Drosophila adult midgut

FBgn0005638

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\slbo

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 62 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of slbo

Transgenic constructs containing regulatory region of slbo

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(2R)BSC780

(Christensen et al., 2009.5.6)

Df(2R)Exel6082

(Ryder, 2004.4.26)

Df(2R)Exel6281

(Ryder, 2004.4.26)

Df(2R)Px2

(Montell et al., 1992, Rorth and Montell, 1992)

In(2LR)Px⁴

(Montell et al., 1992)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | male limited, with Scer\GAL4^Bx-MS1096

slbo^{UAS.ORF.GW.Tag:HA}

lethal | male limited | cold sensitive, with Scer\GAL4^Bx-MS1096

slbo^{UAS.ORF.GW.Tag:HA}

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

slbo^GD4488

lethal - all die during embryonic stage | recessive

Sterility

Allele

female fertile

female sterile

slbo^e7b

female sterile | recessive

semi-sterile

slbo⁰¹³¹⁰

Other Phenotypes

Allele

abnormal cell migration | adult stage, with Scer\GAL4^slbo.2.6

slbo^UAS.cRa

abnormal cell migration | adult stage (with slbo^e7b)

slbo^LY6

abnormal cell migration | adult stage (with slbo^LY6)

slbo^e7b

abnormal developmental rate

slbo⁰¹³¹⁰

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.long

slbo^UAS.cRa

decreased size | adult stage, with Scer\GAL4^GMR.long

slbo^UAS.cRa

some die during embryonic stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

slbo^GD4488

visible, with Scer\GAL4^vg.PM

slbo^UAS.cRa

visible | adult stage, with Scer\GAL4^GMR.long

slbo^UAS.cRa

visible | female limited, with Scer\GAL4^Bx-MS1096

slbo^{UAS.ORF.GW.Tag:HA}

visible | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

slbo^{UAS.ORF.GW.Tag:HA}

wild-type

Phenotype manifest in

Allele

border follicle cell | somatic clone

border follicle cell, with Scer\GAL4^slbo.2.6

slbo^UAS.cRa

border follicle cell (with slbo⁰¹³¹⁰)

border follicle cell (with slbo^e7b)

border follicle cell (with slbo^LY6)

slbo^e7b

border follicle cell (with slbo^ry8ex2d)

slbo⁰¹³¹⁰

border follicle cell | oogenesis stage S10, with Scer\GAL4^slbo.2.6

slbo^UAS.cRa

border follicle cell | oogenesis stage S10 (with slbo^e7b)

slbo^LY6

border follicle cell | oogenesis stage S10 (with slbo^LY6)

slbo^e7b

egg chamber | oogenesis stage S10, with Scer\GAL4^slbo.2.6

slbo^UAS.cRa

egg chamber | oogenesis stage S10 (with slbo^e7b)

slbo^LY6

egg chamber | oogenesis stage S10 (with slbo^LY6)

slbo^e7b

eye, with Scer\GAL4^GMR.long

slbo^UAS.cRa

eye photoreceptor cell | adult stage, with Scer\GAL4^GMR.long

slbo^UAS.cRa

lens, with Scer\GAL4^LL54

slbo^{UAS.ORF.GW.Tag:HA}

lens | somatic clone

slbo^ry8ex22

polar follicle cell

slbo^unspecified

rhabdomere of eye photoreceptor cell | adult stage, with Scer\GAL4^GMR.long

slbo^UAS.cRa

wing, with Scer\GAL4^vg.PM

slbo^UAS.cRa

wing | female limited, with Scer\GAL4^Bx-MS1096

slbo^{UAS.ORF.GW.Tag:HA}

wing | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

slbo^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (20)

Hsap\CEBPA

8 of 14

Yes

Hsap\CEBPB

8 of 14

Yes

Hsap\CEBPD

8 of 14

Yes

Hsap\CEBPE

8 of 14

Yes

Hsap\CEBPG

5 of 14

Hsap\DBP

2 of 14

Hsap\DDIT3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Cebpa

6 of 14

Yes

Rnor\Cebpd

6 of 14

Yes

Rnor\Cebpe

6 of 14

Yes

Rnor\Cebpb

5 of 14

Yes

Rnor\Cebpg

5 of 14

Rnor\Ddit3

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (14)

Mmus\Cebpa

6 of 14

Yes

Mmus\Cebpb

6 of 14

Yes

Mmus\Cebpe

6 of 14

Yes

Mmus\Cebpd

5 of 14

Yes

Mmus\Cebpg

5 of 14

Mmus\Ddit3

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\cebpa

5 of 13

Yes

Xtro\cebpe

4 of 13

Yes

Xtro\cebpb

3 of 13

Yes

Xtro\atf4

1 of 13

Xtro\batf

1 of 13

Xtro\cebpd

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tef

1 of 13

Xtro\thibz

1 of 13

Danio rerio (Zebrafish) (23)

Drer\cebpa

6 of 14

Yes

Drer\cebpb

6 of 14

Yes

Drer\cebpd

5 of 14

Yes

Drer\cebp1

4 of 14

Yes

4 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\cebp-2

3 of 14

Yes

Cele\atf-2

2 of 14

Cele\atf-8

2 of 14

Cele\cebp-1

2 of 14

Yes

Cele\ces-2

2 of 14

Cele\zip-4

1 of 14

Yes

Cele\zip-7

1 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP011096

11 of 12

Yes

3 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (5)

Atha\bZIP16

1 of 13

Yes

Atha\bZIP19

1 of 13

Yes

Atha\bZIP23

1 of 13

Yes

Atha\BZIP24

1 of 13

Yes

Atha\HYH

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\atf1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:slbo. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

Irbp18

3 of 13

CG7786

2 of 13

Pdp1

2 of 13

vri

2 of 13

1 of 13

Mabi

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CEBPA; CCAAT enhancer binding protein alpha

model of acute myeloid leukemia

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CEBPA; CCAAT enhancer binding protein alpha

8 of 14

116897

LEUKEMIA, ACUTE MYELOID; AML

acute myeloid leukemia

CEBPB; CCAAT enhancer binding protein beta

8 of 14

189965

CEBPD; CCAAT enhancer binding protein delta

8 of 14

116898

CEBPE; CCAAT enhancer binding protein epsilon

8 of 14

600749

CEBPG; CCAAT enhancer binding protein gamma

5 of 14

138972

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

fluorescent resonance energy transfer

(Reinke et al., 2013)

slbo - CG7786

fluorescent resonance energy transfer

(Reinke et al., 2013)

slbo - Crg-1

(Shokri et al., 2019)

slbo - Irbp18

(Shokri et al., 2019)

slbo - Lim3

bimolecular fluorescence complementation, fluorescence microscopy

(Shokri et al., 2019)

slbo - Ubx

(Bischof et al., 2018)

slbo - abd-A

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

slbo - crc

fluorescent resonance energy transfer

(Reinke et al., 2013)

slbo - toy

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Shokri et al., 2019)

slbo - trbl

(Fischer et al., 2017, Masoner et al., 2013, Rorth et al., 2000)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

Abl

(Rorth et al., 1998)

enhanceable

aop

(Schober et al., 2005)

suppressible

AP-2α

(Rorth et al., 1998)

suppressible

apt

(Starz-Gaiano et al., 2008)

enhanceable

Blimp-1

(Wang et al., 2022)

enhanceable

btl

(Murphy et al., 1995)

suppressible

btl

(Murphy et al., 1995)

suppressible

EP2585

(Rorth et al., 1998)

suppressible

Hsp27

(Rorth et al., 1998)

suppressible

jing

(Liu and Montell, 2001)

suppressible

mbc

(Duchek et al., 2001)

suppressible

(Rorth, 1994)

suppressible

(Rorth, 1994)

suppressible

(Beccari et al., 2002)

enhanceable

(Gunawan et al., 2013)

suppressible

trbl

(Masoner et al., 2013, Rorth et al., 2000)

suppressible

Usp47

(Rorth et al., 2000)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

apt

suppressible

(Starz-Gaiano et al., 2008)

jar

suppressible

(Geisbrecht and Montell, 2002)

peb

suppressible

(Wilk et al., 2004)

Ras85D

suppressible

(Lee and Montell, 1997)

suppressible