C/EBP, DmC/EBP, slobo
basic leucine zipper - CCAAT/enhancer-binding protein homolog - targets and is essential for terminal differentiation and migration of the anterior follicle cells known as border cells
Gene model reviewed during 5.49
Ubiquitination/deubiquitination regulates border cell migration. Ubiquitination is stimulated by trbl, which leads to proteasomal degradation and inhibits border cell migration. Deubiquitination by Usp47, leads to its stabilization and promotes border cell migration.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\slbo using the Feature Mapper tool.
Expression of slbo is seen in border follicle cells prior to, during and after their migration and in centripetally migrating follicle cells at stage S10.
Transient slbo expression is observed in the centripetally migrating follicle cells at oogenesis stage S10A and is undetectable by stage 10B. Expression in leading edge follicle cells is not observed with the antisera.
Expression of slbo is first detected in border follicle cells in early stage S9 just prior to their migration and in centripetally migrating follicle cells at stage S10 prior to their migration. No staining is visible after stage S10. Staining of border and centripetal follicle cells was greatly reduced or absent in slbo mutants.
Western blots show a peak of slbo protein expression in 12-18hr embryos. Immunolocalization studies show the earliest detectable expression is in the posterior spiracles of 9-10hr embryos. After 12hrs, a rapidly changing pattern is seen. Expression is seen first in the proventriculus, salivary glands and midgut followed by the foregut, hindgut and the epidermis. Epidermal staining is initially fairly ubiquitous but becomes restricted to the ends of the embryo and a few epidermal cells in the intermediate segments.
GBrowse - Visual display of RNA-Seq signalsView Dmel\slbo in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: slbo CG4354
Site directed mutagenesis, protein binding and germline transformation experiments identify and characterise the activity of a simple mini-enhancer from the fat body enhancer (FBE) region consisting of a single binding site (dsxA) for the dsx protein and two others for other regulatory proteins (slbo and ref1). One copy of this enhancer is sufficient to direct the sex and fat body specificities of Yp1 transcription.
A transgenic rescue assay is used to determine which molecular functions of slbo protein are required for it to fulfill its essential role during development.
slbo alleles fully complement bs, l(2)60Ca, l(2)60Cb, l(2)60Cc and l(2)60Cd.
Cloning, expression and sequence analysis of slbo reveals the protein encoded is homologous to the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. Hypomorphic alleles cause the delay of onset of the migration of the border cells (a subset of follicle cells) through the developing egg chamber.
DNA binding, dimerization, and expression studies reveal that the slbo product bears functional as well as structural similarity to mammalian C/EBP.