FlyBase Gene Report: Dmel\Eip63E

General Information

Symbol

Dmel\Eip63E

Species

D. melanogaster

Name

Ecdysone-induced protein 63E

Annotation Symbol

CG10579

Feature Type

protein_coding_gene

FlyBase ID

FBgn0005640

Gene Model Status

Current

Stock Availability

21 publicly available

Gene Snapshot

Ecdysone-induced protein 63E (Eip63E) encodes a cyclin-dependent kinase that interacts with the product of CycY. It contributes to embryogenesis, larval development and metamorphosis. [Date last reviewed: 2019-08-01]

Other Summaries

Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

L63, PFTAIRE, cdc2-63E

Key Links

Genomic Location

Cytogenetic map

63E3-63E5

Sequence location

3L:3,515,751..3,610,521 [+]

Recombination map

3-8

RefSeq locus

NT_037436 REGION:3515751..3610521

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables cyclin binding

inferred from mutant phenotype

(Stowers et al., 2000)

enables cyclin-dependent protein serine/threonine kinase activity

inferred from direct assay

(Davidson et al., 2009)

inferred from mutant phenotype

(Stowers et al., 2000)

enables protein binding

inferred from physical interaction with FLYBASE:Pif2; FB:FBgn0046873

(Rascle et al., 2003)

inferred from physical interaction with FLYBASE:Pif1B; FB:FBgn0046874

(Rascle et al., 2003)

enables protein kinase activity

inferred from direct assay

(Rascle et al., 2003)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables cyclin binding

inferred from biological aspect of ancestor with PANTHER:PTN001172735

(assigned by GO_Central )

(Gaudet et al., 2011)

enables cyclin-dependent protein serine/threonine kinase activity

inferred from sequence or structural similarity with SGD:S000000364

(Stowers et al., 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

enables heme binding

inferred from electronic annotation with InterPro:IPR018506

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables protein serine/threonine kinase activity

inferred from sequence or structural similarity with UniProtKB:Q00536

(Sauer et al., 1996)

Biological Process (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in developmental growth

inferred from mutant phenotype

(Liu and Finley, 2010)

involved_in embryonic development via the syncytial blastoderm

inferred from mutant phenotype

(Stowers et al., 2000)

involved_in instar larval development

inferred from mutant phenotype

(Stowers et al., 2000)

involved_in metamorphosis

inferred from mutant phenotype

(Stowers et al., 2000)

involved_in morphogenesis of an epithelium

inferred from mutant phenotype

(Stowers et al., 2000)

involved_in positive regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Davidson et al., 2009)

involved_in protein phosphorylation

inferred from mutant phenotype

(Sopko et al., 2014)

inferred from direct assay

(Rascle et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein phosphorylation

inferred from sequence or structural similarity with SGD:S000000364

(Stowers et al., 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of transcription involved in G1/S transition of mitotic cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN002373304

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in plasma membrane

inferred from high throughput direct assay

(Lye et al., 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002373304

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN001172735

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES

Pathway (FlyBase)

Negative Regulators of Hippo Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES -: Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose activity depends on a regulatory cyclin subunit. (Adapted from PMID:25180339).

Pathway (FlyBase)

Negative Regulators of Hippo Signaling Pathway -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Summary (Interactive Fly)

cyclin dependent kinase that plays a role in cell differentiation rather than in cell division

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Eip63E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884.

gene_with_stop_codon_read_through ; SO:0000697

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Eip63E-RA

FBtr0073133

NM_168034

2700

509

Eip63E-RB

FBtr0073134

NM_168035

2919

509

Eip63E-RC

FBtr0073135

NM_168036

2891

509

Eip63E-RD

FBtr0073131

NM_079180

3575

522

Eip63E-RE

FBtr0073132

NM_168033

2981

522

Eip63E-RF

FBtr0110920

NM_001043116

3536

509

Eip63E-RG

FBtr0110921

NM_001043120

2958

522

Eip63E-RH

FBtr0110922

NM_001043117

2625

501

Eip63E-RI

FBtr0110923

NM_001043119

2739

522

Eip63E-RJ

FBtr0110924

NM_001043121

4769

509

Eip63E-RK

FBtr0110925

NM_001043118

2930

522

Eip63E-RL

FBtr0301948

NM_001169869

3311

522

Eip63E-RM

FBtr0302598

NM_001202113

4914

509

Eip63E-RN

FBtr0330068

NM_001274444

3575

538

Additional Transcript Data and Comments

Reported size (kB)

3.4 (northern blot)

(Sauer et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Eip63E-PA

FBpp0072992

58.5

509

8.88

NP_728889

AAG22238

Eip63E-PB

FBpp0072993

58.5

509

8.88

NP_728890

AAF47781

Eip63E-PC

FBpp0072994

58.5

509

8.88

NP_728891

AAG22239

Eip63E-PD

FBpp0072990

59.8

522

8.87

NP_523904

AAN11568

Eip63E-PE

FBpp0072991

59.8

522

8.87

NP_728888

AAN11569

Eip63E-PF

FBpp0110220

58.3

509

8.88

NP_001036581

ABI31232

Eip63E-PG

FBpp0110221

59.9

522

8.87

NP_001036585

ABI31236

Eip63E-PH

FBpp0110222

57.7

501

8.88

NP_001036582

ABI31233

Eip63E-PI

FBpp0110223

59.9

522

8.87

NP_001036584

ABI31235

Eip63E-PJ

FBpp0110224

58.5

509

8.88

NP_001036586

ABI31237

Eip63E-PK

FBpp0110225

59.9

522

8.87

NP_001036583

ABI31234

Eip63E-PL

FBpp0291160

59.9

522

8.87

NP_001163340

ACZ94612

Eip63E-PM

FBpp0291754

58.3

509

8.88

NP_001189042

ADV37479

Eip63E-PN

FBpp0303101

61.6

538

9.03

NP_001261373

AGB94068

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

509 aa isoforms: Eip63E-PF, Eip63E-PM

522 aa isoforms: Eip63E-PG, Eip63E-PI, Eip63E-PK, Eip63E-PL

509 aa isoforms: Eip63E-PA, Eip63E-PB, Eip63E-PC, Eip63E-PJ

522 aa isoforms: Eip63E-PD, Eip63E-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

509 (aa)

(Sauer et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eip63E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

posterior midgut primordium

(Sauer et al., 1996)

anterior midgut primordium

(Sauer et al., 1996)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system

(Sauer et al., 1996)

glial cell

(Sauer et al., 1996)

tracheal precursor cell

(Sauer et al., 1996)

myoblast

(Sauer et al., 1996)

embryonic/larval glial cell

(Sauer et al., 1996)

embryonic stage -- adult stage

(Sauer et al., 1996)

Additional Descriptive Data

Eip63E transcripts are detected at all stages of development on northern blots. They are found to be ubiquitous in early embryos by in situ hybridization. They are also present at a low level throughout the embryo at later stages of embryogenesis. Transient strong signals were observed in a number of tissues. These include the anterior and posterior midguts (in cells rearranging cell-cell contacts), tracheal precursors (during invagination), myoblasts (during fusion), and discrete cells of the CNS (which may be migrating glia).

(Sauer et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in plasma membrane

inferred from high throughput direct assay

(Lye et al., 2014)

Expression Deduced from Reporters

Reporter: PBac{681.P.FSVS-1}Eip63E^CPTI001272

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

Comment: not in mushroom body and central complex

(Knowles-Barley, 2011.8.24)

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Eip63E in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Eip63E

View exonic expression by tissue for Dmel\Eip63E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0005640

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0005640

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10038-RB;CG10579-RD

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10579

Flygut - An atlas of the Drosophila adult midgut

FBgn0005640

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Eip63E

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 44 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Eip63E

Transgenic constructs containing regulatory region of Eip63E

Deletions and Duplications ( 7 )

Disrupted in

Df(3L)294

(Munroe et al., 1998.5.12)

Df(3L)663

(Munroe et al., 1998.5.12)

Df(3L)E1

(Munroe et al., 1998.5.12)

Not disrupted in

Df(3L)1-11

(Munroe et al., 1998.5.12)

Df(3L)GN34

(Munroe et al., 1998.5.12)

Df(3L)GN50

(Munroe et al., 1998.5.12)

Df(3L)HR232

(Munroe et al., 1998.5.12)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal (with Eip63E⁸¹)

Eip63E^GN50

lethal (with Eip63E^GN50)

Eip63E⁸¹

lethal | maternal effect (with Eip63E^E1)

Eip63E^GN50

lethal | maternal effect (with Eip63E^GN50)

Eip63E^E1

lethal - all die before end of larval stage | recessive

Eip63E⁸¹

lethal - all die before end of P-stage (with Eip63E⁸¹)

Eip63E^GN50

lethal - all die before end of P-stage (with Eip63E^GN50)

Eip63E⁸¹

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Eip63E^GD16928

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Eip63E^GD16928

viable, with Scer\GAL4^tin.CΔ4

Eip63E^GD16928

viable (with Df(3L)1-11)

Eip63E^GN50

Sterility

Allele

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

fertile

Other Phenotypes

Allele

developmental rate defective (with Eip63E⁸¹)

Eip63E^GN50

developmental rate defective (with Eip63E^GN50)

Eip63E⁸¹

flight defective, with Scer\GAL4^Mef2.PR

Eip63E^GD16928

majority die during larval stage (with Eip63E⁸¹)

Eip63E^GN50

majority die during larval stage (with Eip63E^GN50)

Eip63E⁸¹

small body (with Eip63E^E1)

Eip63E^GN50

small body (with Eip63E^GN50)

Eip63E^E1

small body | pupal stage (with Eip63E⁸¹)

Eip63E^GN50

small body | pupal stage (with Eip63E^GN50)

Eip63E⁸¹

some die during larval stage | recessive

Eip63E⁸¹

some die during P-stage (with Eip63E⁸¹)

Eip63E^GN50

some die during P-stage (with Eip63E^GN50)

Eip63E⁸¹

some die during pupal stage, with Scer\GAL4^pnr-MD237

Eip63E^GD16928

some die during pupal stage (with Eip63E⁸¹)

Eip63E^GN50

some die during pupal stage (with Eip63E^GN50)

Eip63E⁸¹

visible, with Scer\GAL4^pnr-MD237

Eip63E^GD16928

wild-type

Eip63E^B1.hs

Phenotype manifest in

Allele

head | pupal stage (with Eip63E⁸¹)

Eip63E^GN50

head | pupal stage (with Eip63E^GN50)

Eip63E⁸¹

leg (with Df(3L)663)

Eip63E⁸¹

leg | pupal stage (with Eip63E⁸¹)

Eip63E^GN50

leg | pupal stage (with Eip63E^GN50)

Eip63E⁸¹

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Eip63E^GD16928

myofibril, with Scer\GAL4^Mef2.PR

Eip63E^GD16928

polytene chromosome

pupa (with Eip63E⁸¹)

Eip63E^GN50

pupa (with Eip63E^GN50)

Eip63E⁸¹

sarcomere, with Scer\GAL4^Mef2.PR

Eip63E^GD16928

Z disc, with Scer\GAL4^Mef2.PR

Eip63E^GD16928

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CDK14

14 of 15

Yes

Hsap\CDK15

11 of 15

Yes

Hsap\CDK17

5 of 15

Yes

Hsap\CDK2

4 of 15

Hsap\CDK16

4 of 15

Yes

Hsap\CDK18

4 of 15

Yes

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (15)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cdk14

13 of 15

Yes

Mmus\Cdk15

10 of 15

Yes

Mmus\Cdk17

5 of 15

Yes

Mmus\Cdk2

4 of 15

Mmus\Cdk16

4 of 15

Yes

Mmus\Cdk18

4 of 15

Yes

3 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (12)

Rnor\Cdk14

9 of 13

Yes

Rnor\Cdk17

5 of 13

Yes

Rnor\Cdk18

5 of 13

Yes

Rnor\Cdk2

4 of 13

Rnor\Cdk16

4 of 13

Yes

Rnor\Cdk1

3 of 13

Rnor\Cdk15

3 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (12)

Xtro\cdk14

13 of 12

Yes

Xtro\cdk15

7 of 12

Yes

Xtro\cdk17

5 of 12

Yes

Xtro\cdk2

4 of 12

Xtro\cdk18

4 of 12

Yes

Xtro\cdk1

3 of 12

Xtro\cdk16

3 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (13)

Drer\cdk15

11 of 15

Yes

Drer\cdk14

10 of 15

Yes

Drer\cdk17

5 of 15

Yes

Drer\cdk2

4 of 15

Drer\cdk16

4 of 15

Yes

Drer\si:dkey-166c18.1

4 of 15

Yes

Drer\cdk1

3 of 15

Drer\LOC100536316

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\ZC123.4

9 of 15

Yes

Cele\pct-1

7 of 15

Yes

3 of 15

2 of 15

2 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (6)

Atha\CDC2

1 of 9

Yes

Atha\CDKB1;1

1 of 9

Yes

Atha\CDKB1;2

1 of 9

Yes

Atha\CDKB2;1

1 of 9

Yes

Atha\CDKB2;2

1 of 9

Yes

Atha\MPK15

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\PHO85

5 of 15

Yes

Scer\CDC28

2 of 15

Scer\CAK1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\pef1

3 of 12

Yes

Spom\cdc2

1 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905UN )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10409

Drosophila persimilis

Dper\GL15117

Drosophila willistoni

Dwil\GK20517

Drosophila virilis

Dvir\GJ12403

Drosophila mojavensis

Dmoj\GI12497

Drosophila grimshawi

Dgri\GH16418

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505MO )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17007266

Glossina morsitans

Tsetse fly

Gmor\GMOY003057

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_01181

Mayetiola destructor

Hessian fly

Mdes\Mdes002710

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL015000

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL012915

Anopheles darlingi

American malaria mosquito

Adar\ADAC004645

Anopheles gambiae

Malaria mosquito

Agam\AGAP005729

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008438

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06DD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA011854

Danaus plexippus

Monarch butterfly

Dple\DPOGS201944-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002219

Apis florea

Little honeybee

Aflo\2963320724

Apis mellifera

Western honey bee

Amel\GB44292

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP14680

Bombus terrestris

Buff-tailed bumblebee

Bter\15209785

Linepithema humile

Argentine ant

Lhum\LH10792

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000d9b

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG024622

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_09865

Tribolium castaneum

Red flour beetle

Tcas\TC002523

Pediculus humanus

Human body louse

Phum\PHUM321170

Rhodnius prolixus

Kissing bug

Rpro\RPRC002851

Cimex lectularius

Bed bug

Clec\CLEC004419

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004749

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_12838

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04PW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008911

Strigamia maritima

European centipede

Smar\SMAR008466

Ixodes scapularis

Black-legged tick

Isca\ISCW024673

Ixodes scapularis

Black-legged tick

Isca\ISCW001031

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56853.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56761.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur38g00630

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_299555

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_54893

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0CH0 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pctaire

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001227gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pftaire-1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk2_1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000006693

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005978

Ciona intestinalis

Vase tunicate

Cint\LOC100182544

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009174

Gallus gallus

Domestic chicken

Ggal\CDK17

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027196

Gallus gallus

Domestic chicken

Ggal\CDK14

Gallus gallus

Domestic chicken

Ggal\CDK15

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028442

Gallus gallus

Domestic chicken

Ggal\CDC2

Gallus gallus

Domestic chicken

Ggal\CDK3

Gallus gallus

Domestic chicken

Ggal\CDK18

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (14)

Gene Symbol

Score

Source

Alignment

5 of 10

5 of 10

4 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CDK14; cyclin dependent kinase 14

14 of 15

610679

CDK15; cyclin dependent kinase 15

11 of 15

616147

CDK17; cyclin dependent kinase 17

5 of 15

603440

CDK2; cyclin dependent kinase 2

4 of 15

116953

CDK16; cyclin dependent kinase 16

4 of 15

311550

CDK18; cyclin dependent kinase 18

4 of 15

169190

CDK1; cyclin dependent kinase 1

3 of 15

116940

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Eip63E - CycC

two hybrid

(Liu and Finley, 2010)

Eip63E - CycY

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Liu and Finley, 2010)

Eip63E - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63926

DroID - A comprehensive database of gene and protein interactions.

FBgn0005640

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38433

MIST (protein-protein) - An integrated Molecular Interaction Database

Eip63E

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Hippo Signaling Pathway -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-8

Cytogenetic map

63E3-63E5

Sequence location

3L:3,515,751..3,610,521 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63E3-63E5

Limits computationally determined from genome sequence between P{PZ}kst⁰¹³¹⁸ and P{PZ}Sc2⁰⁵⁶³⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

63E-63E

(Lehner, 1996.7.23)

63E-63E

(determined by in situ hybridisation)

(Sauer et al., 1996)

Experimentally Determined Recombination Data

Location

3-8

(FlyBase, 2016)

3-7.1

(Comeron et al., 2012)

(Lehner, 1996.7.23)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Bloomington

17151

w¹¹¹⁸; P{EP}Eip63E^EP3656

28901

y¹ v¹; P{TRiP.HM05111}attP2

4513

w^*; Eip63E⁸¹/TM6B, Tb¹

37797

y¹ w^*; Mi{MIC}Eip63E^MI03882

52057

y¹ w^*; Mi{MIC}Eip63E^MI08829

16218

y¹ w¹¹¹⁸; PBac{5HPw⁺}Eip63E^B417

16281

y¹ w¹¹¹⁸; PBac{3HPy⁺}Eip63E^C107

Kyoto

115141

w¹¹¹⁸; PBac{681.P.FSVS-1}Eip63E^CPTI001272

VDRC

v106824

P{KK108615}VIE-260B

v47860

w¹¹¹⁸; P{GD16928}v47860

v50599

w¹¹¹⁸; P{GD16928}v50599/TM3

v342011

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01362}attP40/CyO-GFP

More stocks available...

Genomic Clones (74)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (318)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

GH02721

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0005640

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0005640

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38433

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Eip63E CG10579

(Li, 1999.11)

Source for database merge of

Source for merge of: Eip63E Pftaire

(Munroe et al., 1998.5.12)

Additional comments

Other Comments

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a decrease in mitotic index is seen.

(Bettencourt-Dias et al., 2004)

Eip63E is required not only for metamorphosis, but also maternally and for embryonic and larval development.

(Stowers et al., 2000)

Characterisation of Eip63E.

(Sauer et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 123 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38433

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0005640

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0005640

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10579

Flygut - An atlas of the Drosophila adult midgut

FBgn0005640

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38433

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10579

modMine - A data warehouse for the modENCODE project

FBgn0005640

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q7KM03

Linkouts

BioGRID - A database of protein and genetic interactions.

63926

DroID - A comprehensive database of gene and protein interactions.

FBgn0005640

DRSC - Results frm RNAi screens

FBgn0005640

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0005640

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10038-RB;CG10579-RD

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0005640

FlyMine - An integrated database for Drosophila genomics

FBgn0005640

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38433

MIST (protein-protein) - An integrated Molecular Interaction Database

Eip63E

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG10579

(Ashraf et al., 2020, Neuert et al., 2017, Hosono et al., 2015, Christis and Munro, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Morrison et al., 2000)

EIP63E

(Ritter and Beckstead, 2010)

L63

(Deng and Kerppola, 2013, Davidson et al., 2009, Rascle et al., 2003, Stowers et al., 2000, Rascle and Hogness, 1999, Munroe et al., 1998.5.12)

PFTAIRE

(Bjorklund et al., 2006)

Pftaire

(Xie et al., 2015)

cdc2-63E

(Richardson, 2005.2.11, Brumby et al., 2004)

Name Synonyms

Ecdysone-induced protein 63E

(Hueber et al., 2007, Burnette et al., 2005)

PFTAIRE

(Morrison et al., 2000, Lehner, 1996.7.23, Sauer et al., 1996)

Pftaire

(Liu and Kipreos, 2000)

Secondary FlyBase IDs

FBgn0016695
FBgn0024942

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (116)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections