FlyBase Gene Report: Dmel\Pabp2

General Information

Symbol

Dmel\Pabp2

Species

D. melanogaster

Name

Pabp2

Annotation Symbol

CG2163

Feature Type

protein_coding_gene

FlyBase ID

FBgn0005648

Gene Model Status

Current

Stock Availability

16 publicly available

Gene Snapshot

Pabp2 (Pabp2) encodes a nuclear poly(A) binding protein that is involved in both the cleavage and polyadenylation steps of the nuclear cleavage/polyadenylation reaction. Cytoplasmic protein encoded by Pabp2 acts to shorten the poly(A) tails of specific mRNAs in oocytes and syncytial blastoderm embryos. [Date last reviewed: 2018-09-13]

Other Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

PABPN1, rox2

Key Links

Genomic Location

Cytogenetic map

44B3-44B3

Sequence location

2R:8,131,431..8,134,374 [+]

Recombination map

2-58

RefSeq locus

NT_033778 REGION:8131431..8134374

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables poly(A) binding

inferred from direct assay

(assigned by UniProt )

(Benoit et al., 1999)

enables protein binding

inferred from physical interaction with UniProtKB:Q9VSK8

(assigned by UniProt )

(Hurt et al., 2009)

enables RNA binding

inferred from direct assay

(assigned by UniProt )

(Benoit et al., 1999)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

enables poly(A) binding

inferred from sequence or structural similarity with SGD:S000005075

(Mount and Salz, 2000)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in mRNA polyadenylation

inferred from mutant phenotype

(Benoit et al., 2005)

inferred from direct assay

(assigned by UniProt )

(Benoit et al., 1999)

involved_in nuclear-transcribed mRNA poly(A) tail shortening

inferred from mutant phenotype

(Benoit et al., 2005)

involved_in positive regulation of protein glycosylation

inferred from mutant phenotype

(assigned by UniProt )

(Yamamoto-Hino et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mRNA splicing, via spliceosome

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Benoit et al., 1999)

inferred from direct assay

(Benoit et al., 2005)

located_in nucleus

inferred from direct assay

(Chartier et al., 2006, Benoit et al., 2005)

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(assigned by UniProt )

(Benoit et al., 1999)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (PubMed:10481015). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Binds to poly(A) and to poly(G) with high affinity (PubMed:10481015). May protect the poly(A) tail from degradation (By similarity). Plays a role in the positive regulation of alpha-1,3 fucosylation, possibly by cooperating with swm which regulates nuclear export of fucosyltransferase FucTA (PubMed:21203496).

(UniProt, Q7KNF2)

Summary (Interactive Fly)

poly(A) binding protein - functions in nuclear polyadenylation - cytoplasmic PABP2 acts to shorten the poly(A) tails of specific mRNAs

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pabp2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pabp2-RA

FBtr0088785

NM_057554

1869

224

Pabp2-RB

FBtr0088786

NM_165589

1378

224

Additional Transcript Data and Comments

Reported size (kB)

1.7, 1.3, 1.0 (northern blot)

(Benoit et al., 1999)

1.757 (longest cDNA)

(Brand et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Pabp2-PA

FBpp0087863

25.0

224

4.88

NP_476902

AAF59127

Pabp2-PB

FBpp0087864

25.0

224

4.88

NP_724648

AAM68852

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

224 aa isoforms: Pabp2-PA, Pabp2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

224 (aa); 33 (kD observed)

(Benoit et al., 1999)

224 (aa); 25 (kD predicted)

(Brand et al., 1995)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with ZC3H3.

(UniProt, Q7KNF2)

Domain

The RRM domain is essential for specific adenine bases recognition in the poly(A) tail but not sufficient for poly(A) binding.

(UniProt, Q7KNF2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pabp2 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

ovary

(Benoit et al., 1999)

embryonic stage -- adult stage

(Benoit et al., 1999)

larval stage & pupal stage & adult stage

(Benoit et al., 1999)

larval stage

(Benoit et al., 1999)

pupal stage

(Benoit et al., 1999)

embryonic stage 9 -- 16

organism

(Benoit et al., 1999)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female & oogenesis

female germline stem cell

(Benoit et al., 1999)

follicle cell

(Benoit et al., 1999)

female germline stem cell

• nucleus

(Benoit et al., 1999)

follicle cell

• nucleus

(Benoit et al., 1999)

embryonic stage

(Benoit et al., 1999)

larval stage

(Benoit et al., 1999)

embryonic stage 1 -- 2

organism

• cytoplasm

(Benoit et al., 1999)

embryonic stage 3 -- 4

organism

• cytoplasm & nucleus

(Benoit et al., 1999)

embryonic stage 5

cell

• nucleus

(Benoit et al., 1999)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Benoit et al., 1999)

inferred from direct assay

(Benoit et al., 2005)

located_in nucleus

inferred from direct assay

(Chartier et al., 2006, Benoit et al., 2005)

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(assigned by UniProt )

(Benoit et al., 1999)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

Reporter: P{PTT-GC}Pabp2^CC00380

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

female germline cell

(Neumüller et al., 2012)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pabp2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pabp2

View exonic expression by tissue for Dmel\Pabp2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0005648

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0005648

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2163-RB;CG2163-RB;CG2163-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2163

Flygut - An atlas of the Drosophila adult midgut

FBgn0005648

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pabp2

Transgenic constructs containing regulatory region of Pabp2

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation

Gene disrupted in

Df(2R)BSC266

(Christensen et al., 2008.12.28)

Df(2R)Exel6056

(Kahsai et al., 2016, Ryder, 2004.4.26)

Df(2R)Exel7095

(Ryder, 2004.4.26)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Pabp2^GD9743

lethal (with Df(2R)Exel6056)

Pabp2⁵⁵

lethal | female limited, with Scer\GAL4^dome-PG14

Pabp2^HMS00553

lethal | first instar larval stage (with Df(2R)CA53)

Pabp2^EP2264

lethal | first instar larval stage | recessive

lethal | recessive

Pabp2⁰¹

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

Pabp2^GD9743

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Pabp2^GD9743

partially lethal - majority die | embryonic stage | germline clone | maternal effect

Pabp2^EP2264

partially lethal - majority die | embryonic stage | maternal effect

Pabp2⁶

partially lethal - majority die | late embryonic stage (with Df(2R)CA53)

Pabp2^EP2264

partially lethal - majority die | late embryonic stage | recessive

Pabp2⁵⁵

partially lethal - majority die | recessive

Pabp2⁶

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pabp2^GD9743

viable, with Scer\GAL4^tin.CΔ4

Pabp2^GD9743

Sterility

Allele

female semi-sterile, with Scer\GAL4^VP16.otu

Pabp2^HMS00553

female semi-sterile | recessive

Pabp2⁶

Other Phenotypes

Allele

abnormal locomotor behavior | larval stage

Pabp2⁵⁵

abnormal mitotic cell cycle | embryonic stage | germline clone | maternal effect

Pabp2⁶

abnormal mitotic cell cycle | embryonic stage | maternal effect

Pabp2⁶

abnormal neuroanatomy | larval stage

Pabp2^GD9743

neoplasia | adult stage | male, with Scer\GAL4^VP16.bam

some die during pupal stage, with Scer\GAL4^pnr-MD237

Pabp2^GD9743

visible, with Scer\GAL4^nub-AC-62

Pabp2^HMS00553

Phenotype manifest in

Allele

egg chamber | oogenesis stage S8

Pabp2⁶

egg chorion

Pabp2⁶

egg chorion | germline clone

Pabp2^EP2264

embryo | germline clone | maternal effect

Pabp2^EP2264

embryo | maternal effect

Pabp2⁶

female germline cell, with Avic\GFP^{dsRNA.shRNA.UAS.V22.1}, Scer\GAL4^VP16.nos.UTR

Pabp2^CC00380

ganglion mother cell | larval stage

Pabp2^GD9743

larval neuroblast | larval stage

Pabp2^GD9743

sarcomere, with Scer\GAL4^Mef2.PR

Pabp2^GD9743

spermatogonium, with Scer\GAL4^VP16.bam

testis, with Scer\GAL4^VP16.bam

wing vein, with Scer\GAL4^nub-AC-62

Pabp2^HMS00553

Z disc, with Scer\GAL4^Mef2.PR

Pabp2^GD9743

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PABPN1

14 of 15

Yes

Hsap\PABPN1L

6 of 15

Yes

Hsap\BCL2L2-PABPN1

3 of 15

Yes

Hsap\RNPS1

1 of 15

Hsap\RORB

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Pabpn1

13 of 15

Yes

Mmus\Pabpn1l

4 of 15

Yes

Mmus\Bcl2l2

2 of 15

Yes

Mmus\Rnps1

1 of 15

Rattus norvegicus (Norway rat) (3)

Rnor\Pabpn1

12 of 13

Yes

Rnor\Pabpn1l

6 of 13

Yes

Rnor\Rnps1

1 of 13

Xenopus tropicalis (Western clawed frog) (3)

Xtro\pabpn1

11 of 12

Yes

Xtro\pabpn1l

7 of 12

Yes

Xtro\rnps1

1 of 12

Danio rerio (Zebrafish) (3)

Drer\pabpn1

13 of 15

Yes

Drer\pabpn1l

7 of 15

Yes

Drer\rnps1

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\pabp-2

14 of 15

Yes

Cele\EEED8.4

5 of 15

Yes

Cele\EEED8.12

5 of 15

Yes

Cele\sgnh-1

5 of 15

Yes

Cele\C38C6.5

1 of 15

Yes

Cele\rnp-5

1 of 15

Cele\T08B6.1

1 of 15

Yes

Cele\T08B6.9

1 of 15

Yes

Cele\Y51H4A.22

1 of 15

Yes

Cele\Y51H4A.23

1 of 15

Yes

Cele\ZK1225.4

1 of 15

Yes

Cele\ZK1225.5

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (5)

Atha\AT5G65260

9 of 9

Yes

Atha\AT5G10350

8 of 9

Yes

Atha\PABN1

8 of 9

Yes

Atha\AT1G72800

1 of 9

Yes

Atha\AT5G03480

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\SGN1

11 of 15

Yes

Scer\TIF3

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\pab2

10 of 12

Yes

Spom\SPBC365.04c

1 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190GWN )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15278

Drosophila persimilis

Dper\GL17107

Drosophila willistoni

Dwil\GK23192

Drosophila virilis

Dvir\GJ22134

Drosophila mojavensis

Dmoj\GI19259

Drosophila grimshawi

Dgri\GH21908

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150CKP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17023862

Glossina morsitans

Tsetse fly

Gmor\GMOY000891

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_12087

Mayetiola destructor

Hessian fly

Mdes\Mdes004695

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL015143

Anopheles darlingi

American malaria mosquito

Adar\ADAC001476

Anopheles gambiae

Malaria mosquito

Agam\AGAP005117

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004581

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0FQT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001950

Danaus plexippus

Monarch butterfly

Dple\DPOGS214678-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007685

Apis florea

Little honeybee

Aflo\3026265012

Apis mellifera

Western honey bee

Amel\Pabp2

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13694

Bombus terrestris

Buff-tailed bumblebee

Bter\15209535

Linepithema humile

Argentine ant

Lhum\LH13886

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001232

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001103

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08772

Tribolium castaneum

Red flour beetle

Tcas\TC009984

Pediculus humanus

Human body louse

Phum\PHUM037550

Rhodnius prolixus

Kissing bug

Rpro\pAbp2

Cimex lectularius

Bed bug

Clec\CLEC003680

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000613

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08112

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0FQT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008743

Strigamia maritima

European centipede

Smar\SMAR000472

Strigamia maritima

European centipede

Smar\SMAR012132

Strigamia maritima

European centipede

Smar\SMAR007358

Strigamia maritima

European centipede

Smar\SMAR012149

Strigamia maritima

European centipede

Smar\SMAR011337

Stegodyphus mimosarum

African social velvet spider

Smim\KFM75551.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78317.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur28g02200

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_206317

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0PAK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pabpn1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000012468

Gallus gallus

Domestic chicken

Ggal\PABPN1L

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

oculopharyngeal muscular dystrophy

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PABPN1; poly(A) binding protein nuclear 1

model of oculopharyngeal muscular dystrophy

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Pabp2^EP2264

ameliorates autosomal recessive non-syndromic intellectual disability

modeled by Nab2^ex3

(Pak et al., 2011)

Pabp2⁵⁵

exacerbates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.YFP.cEa

(Chou et al., 2015)

modeled by Hsap\TARDBP^{A315T.UAS.YFP}

(Chou et al., 2015)

modeled by Hsap\TARDBP^{D169G.UAS.YFP}

(Chou et al., 2015)

modeled by Hsap\TARDBP^{G298S.UAS.YFP}

(Chou et al., 2015)

ameliorates oculopharyngeal muscular dystrophy

modeled by Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

exacerbates autosomal recessive non-syndromic intellectual disability

modeled by Nab2^ex3

(Pak et al., 2011)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PABPN1; poly(A) binding protein nuclear 1

14 of 15

602279

OCULOPHARYNGEAL MUSCULAR DYSTROPHY; OPMD

oculopharyngeal muscular dystrophy

PABPN1L; PABPN1 like, cytoplasmic

6 of 15

BCL2L2-PABPN1; BCL2L2-PABPN1 readthrough

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pabp2 - ZC3H3

anti tag coimmunoprecipitation, anti tag western blot, tandem affinity purification, Identification by mass spectrometry

(Hurt et al., 2009)

Pabp2 - d

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Pabp2 - orb2

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Khan et al., 2015, White-Grindley et al., 2014)

RNA-protein

Physical Interaction

Assay

References

Pabp2 - tut

rna three hybrid

(Shan et al., 2017)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

dom

enhanceable

Pabp2

(Kwon et al., 2013)

mam

enhanceable

Pabp2

(Kwon et al., 2013)

suppressible

(Pak et al., 2011)

enhanceable

(Pak et al., 2011)

suppressible

(Benoit et al., 2005)

Rbf

enhanceable

Pabp2

(Kwon et al., 2013)

External Data

Subunit Structure (UniProtKB)

Interacts with ZC3H3.

(UniProt, Q7KNF2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61644

DroID - A comprehensive database of gene and protein interactions.

FBgn0005648

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35788

MIST (genetic) - An integrated Molecular Interaction Database

Pabp2

MIST (protein-protein) - An integrated Molecular Interaction Database

Pabp2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

mRNA surveillance pathway

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-58

Cytogenetic map

44B3-44B3

Sequence location

2R:8,131,431..8,134,374 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

44B3-44B3

Limits computationally determined from genome sequence between P{lacW}Vps28^k16503 and P{EP}kermit^EP493

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

44B-44B

(Bourbon, 1998.12.23)

Experimentally Determined Recombination Data

Location

2-58

(FlyBase, 2016)

2-57.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Bloomington

38391

w^*; Pabp2⁶/CyO

38390

Pabp2⁵⁵/CyO, P{ActGFP}JMR1

13212

y¹; P{SUPor-P}Pabp2^KG02359/CyO; ry⁵⁰⁶

9838

y¹ w^*; P{UAST-YFP.RabX1.T19N}Pabp2⁰¹/SM5

77016

y¹ sc^* v¹ sev²¹; P{TKO.GS00790}attP40

78640

y¹ sc^* v¹ sev²¹; P{TOE.GS01882}attP40

FlyORF

F001584

M{UAS-Pabp2.ORF}ZH-86Fb

F000829

M{UAS-Pabp2.ORF.3xHA}ZH-86Fb

Kyoto

110958

w^* P{PTT-GA}Pabp2^ZCL3178

VDRC

v106466

P{KK102711}VIE-260B

v33499

w¹¹¹⁸; P{GD9743}v33499

v318148

PBac{fTRG00565.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (98)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE69521

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0005648

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0005648

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35788

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Na et al., 2016)

polyclonal

(Benoit et al., 1999)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Rox2 CG2163

(Heiman, 1999.11)

Source for database merge of

Additional comments

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Pabp2 stimulates poly(A) polymerase and is able to control poly(A) tail length in vitro.

(Benoit et al., 1999)

Pabp2 is identified as an RRM-type RNA-binding protein that may control important aspects of development.

(Brand et al., 1995)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35788

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q7KNF2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E2QC83

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0005648

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0005648

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2163

Flygut - An atlas of the Drosophila adult midgut

FBgn0005648

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35788

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009984

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2163

modMine - A data warehouse for the modENCODE project

FBgn0005648

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

13212-Pabp2[KG02359]

BioGRID - A database of protein and genetic interactions.

61644

DPiM - Drosophila Protein interaction map

FBgn0005648

DroID - A comprehensive database of gene and protein interactions.

FBgn0005648

DRSC - Results frm RNAi screens

FBgn0005648

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0005648

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2163-RB;CG2163-RB;CG2163-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0005648

FlyMine - An integrated database for Drosophila genomics

FBgn0005648

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35788

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

mRNA surveillance pathway

MIST (genetic) - An integrated Molecular Interaction Database

Pabp2

MIST (protein-protein) - An integrated Molecular Interaction Database

Pabp2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG2163

(Na et al., 2016, Horan et al., 2015, Hosono et al., 2015, Fukuoh et al., 2014, White-Grindley et al., 2014, Schertel et al., 2013, Ellis and Carney, 2010, Ni et al., 2010.12.1, Yamamoto-Hino et al., 2010, Herold et al., 2009, Keleman et al., 2009.8.5, Behm-Ansmant et al., 2007, Quinones-Coello, 2007, Mount and Salz, 2000)

PABP2

(Potikanond et al., 2018, Chou et al., 2015, Bradley et al., 2012, Chartier et al., 2006)

PABPN1

(Singer and Gall, 2011, Behm-Ansmant et al., 2007)

Pabp2

(Meltzer et al., 2019, Podolsky et al., 2018, Shan et al., 2017, Transgenic RNAi Project members, 2017-, Na et al., 2016, Rathore et al., 2016, Sarov et al., 2016, Sysoev et al., 2016, Chartier et al., 2015, Horan et al., 2015, Khan et al., 2015, White-Grindley et al., 2014, Kwon et al., 2013, Kwon et al., 2013, Schertel et al., 2013, Zhan et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Neumüller et al., 2012, Lindquist et al., 2011, Neumüller et al., 2011, Pak et al., 2011, Waldholm et al., 2011, Ellis and Carney, 2010, Yamamoto-Hino et al., 2010, Clements et al., 2009, Herold et al., 2009, Hurt et al., 2009, Christensen et al., 2008.12.28, Farny et al., 2008, McDermott and Kliman, 2008, Buszczak et al., 2007, Quinones-Coello, 2007, Stuart et al., 2007, Zhang et al., 2007, Larsen et al., 2006, Liebl et al., 2006, Benoit et al., 2005, Staudt et al., 2005)

ROX2

(Brand et al., 1995)

Rox2

dpabII

(Benoit et al., 1999)

pAbp2

(Lasko, 2000)

pabp2

(Lefebvre et al., 2016, Czech et al., 2013, Kwon et al., 2013, Singer and Gall, 2011, Sofola et al., 2007, Clyne et al., 2003)

rox2

(Temme et al., 2009, Brand, 1994)

Name Synonyms

Pabp2

nuclear poly(A)-binding protein

(Temme et al., 2009)

poly-A binding protein 2

(Zhan et al., 2013)

polyA-binding protein II

(Benoit et al., 1999)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (108)

The following external sites may use different assemblies or annotations than FlyBase.

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